Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 426,157 G→T 100% E33* (GAG→TAG)  yajD → HNH nuclease family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273426,1570GT93.3% 468.4 / ‑0.1 197E33* (GAG→TAG) yajDHNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base G (20/0);  new base T (79/98);  total (99/98)
Fisher's exact test for biased strand distribution p-value = 6.57e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT  >  CP009273/426013‑426300
                                                                                                                                                |                                                                                                                                               
aTCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgt                                                                                                                                              <  1:2513216/148‑1 (MQ=255)
aTCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgt                                                                                                                                              <  1:547849/148‑1 (MQ=255)
  cTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAgttt                                                                                                                                            >  1:2767930/1‑148 (MQ=255)
      ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgttt                                                                                                                                        <  2:812406/148‑1 (MQ=255)
       gAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTa                                                                                                                                       <  1:3498440/148‑1 (MQ=255)
       gAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTa                                                                                                                                       <  2:1550924/148‑1 (MQ=255)
        aaaCTGCGCGGAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTAt                                                                                                                                      <  2:3042534/148‑1 (MQ=255)
          aCTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTc                                                                                                                                    >  2:1667098/1‑148 (MQ=255)
           cTGCGCGTAACACTACATTTCATCAAGGTAACAATTCATGGATATCATCCACAAAAAATATGCGCGGTTACAAAGCGGCTATAGCGAAAAAGAATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTATTTTATTcc                                                                                                                                   >  2:1822899/1‑148 (MQ=255)
            tGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCa                                                                                                                                  <  2:1051028/148‑1 (MQ=255)
            tGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCa                                                                                                                                  <  2:1095007/148‑1 (MQ=255)
                 gTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTg                                                                                                                             <  2:783195/148‑1 (MQ=255)
                 gTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTg                                                                                                                             <  2:21952/148‑1 (MQ=255)
                 gTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTg                                                                                                                             <  2:161200/148‑1 (MQ=255)
                  tAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTTTTACAACCTGc                                                                                                                            >  1:379923/1‑148 (MQ=255)
                   aaCCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCg                                                                                                                           <  2:3019054/148‑1 (MQ=255)
                   aaCCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCg                                                                                                                           <  2:2328070/148‑1 (MQ=255)
                     cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTg                                                                                                                         >  1:1331883/1‑148 (MQ=255)
                     cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTg                                                                                                                         >  1:1834220/1‑148 (MQ=255)
                        tACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGGTTGTTTTTTCCAACCTGCGTGTAc                                                                                                                      >  1:3431082/1‑148 (MQ=255)
                        tACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCGGTTGCCGCGAGTTTGGTTATTGCAACCTGCGTGAAc                                                                                                                      >  1:1552803/1‑148 (MQ=255)
                         aCATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACt                                                                                                                     <  1:496941/148‑1 (MQ=255)
                          cATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACtt                                                                                                                    >  2:1037486/1‑148 (MQ=255)
                              tCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCg                                                                                                                >  2:1596814/1‑148 (MQ=255)
                              tCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCg                                                                                                                <  2:2438382/148‑1 (MQ=255)
                                aTCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGtt                                                                                                              >  2:2783899/1‑148 (MQ=255)
                                 tCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTc                                                                                                             >  2:965160/1‑148 (MQ=255)
                                  ccAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTca                                                                                                            >  2:119502/1‑148 (MQ=255)
                                      gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTGCAACCTGCGTGAACTTACCGTTaaccac                                                                                                        >  1:686068/1‑148 (MQ=255)
                                      gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTcaccac                                                                                                        >  2:724645/1‑148 (MQ=255)
                                      gTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTcaccac                                                                                                        >  2:3198017/1‑148 (MQ=255)
                                        aaCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAt                                                                                                      <  1:1034724/148‑1 (MQ=255)
                                          cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTg                                                                                                    <  2:2420456/148‑1 (MQ=255)
                                          cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTg                                                                                                    <  2:2522719/148‑1 (MQ=255)
                                          cTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGGTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTg                                                                                                    >  2:441604/1‑148 (MQ=255)
                                           ttttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCAGCGTGAACGTACCGTTCACCACAATGa                                                                                                   >  1:807250/1‑148 (MQ=255)
                                            tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                  <  1:3373246/148‑1 (MQ=255)
                                            tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                  <  1:2704082/148‑1 (MQ=255)
                                                aTGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACg                                                                                              <  2:2639509/148‑1 (MQ=255)
                                                aTGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACg                                                                                              >  2:1108702/1‑148 (MQ=255)
                                                  ggCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAAACTGCGTGAACTTACCGTTAACCACCATGATCACGAc                                                                                            >  1:191330/1‑148 (MQ=255)
                                                    cTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTCTCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTCGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCa                                                                                          >  2:653421/1‑148 (MQ=255)
                                                     tATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCAt                                                                                         <  1:1595755/148‑1 (MQ=255)
                                                        catcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATAcc                                                                                      >  1:854293/1‑148 (MQ=255)
                                                           cccAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTTTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTAACCACATTGATCACGACCATACCAAt                                                                                   >  2:886861/1‑148 (MQ=255)
                                                           cccAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACAATTATAACGACCAAACCAAt                                                                                   >  1:625009/1‑148 (MQ=255)
                                                               aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTTGTTTGTTTATTACAACCTGCGTGAACTTACCGGTCACCACATTGATCACGCCCATTCCAATAAcc                                                                               >  1:181564/1‑148 (MQ=255)
                                                               aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAAcc                                                                               <  1:2833501/148‑1 (MQ=255)
                                                               aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAAcc                                                                               <  1:2063346/148‑1 (MQ=255)
                                                                 aaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCg                                                                             >  2:3435934/1‑148 (MQ=255)
                                                                  aaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCgg                                                                            >  1:3123618/1‑148 (MQ=255)
                                                                       tGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGAt                                                                       <  2:2912641/148‑1 (MQ=255)
                                                                        gcgcGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCAGACCAATAACCCGGAAGATg                                                                      <  2:2723663/148‑1 (MQ=255)
                                                                         cgcgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATgg                                                                     <  1:95513/148‑1 (MQ=255)
                                                                          gcgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGc                                                                    <  2:1296827/148‑1 (MQ=255)
                                                                              ttAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTGGCGCGAGGTTTTTTATTACAAACATCGTGAACTTAACCTTAAACACAATGATCACCACCATACCAAAAAACCCGGACATGGCAGTa                                                                >  1:597191/1‑148 (MQ=255)
                                                                              ttAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTa                                                                <  1:2159398/148‑1 (MQ=255)
                                                                               tAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAACTTACCGTTCACCACAATGATACCGACCATACCAAAAACCCGGAAGATGGCAGTaa                                                               >  1:1310670/1‑148 (MQ=255)
                                                                                 gAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACt                                                             >  2:134219/1‑148 (MQ=255)
                                                                                  aaaGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTg                                                            <  2:550937/148‑1 (MQ=255)
                                                                                    aGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTggg                                                          <  2:655943/148‑1 (MQ=255)
                                                                                        gCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCCACCTGCGTGAACTTACCGTTCACCACATTTATCACGACCAAAACAATAAACCGGAAGAAGGCAGTAACTGGGAAtt                                                      >  1:1658231/1‑148 (MQ=255)
                                                                                           aTCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAttgtt                                                   <  2:2049043/148‑1 (MQ=255)
                                                                                           aTCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAttgtt                                                   <  1:15224/148‑1 (MQ=255)
                                                                                            tCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAttgttg                                                  >  1:1164079/1‑148 (MQ=255)
                                                                                               cgAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTgtgt                                               <  1:2889311/148‑1 (MQ=255)
                                                                                                 aaaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtct                                             <  1:2548615/148‑1 (MQ=255)
                                                                                                 aaaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCGGCGTGAACTTACCGTTCACCACAATGATCACGACCATACCAAAAACCCGGCAGATGGCCGTAACAGGGCATTGTTGTGtct                                             >  1:169940/1‑148 (MQ=255)
                                                                                                 aaaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtct                                             >  1:1944483/1‑148 (MQ=255)
                                                                                                  aaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtctc                                            <  1:716205/148‑1 (MQ=255)
                                                                                                  aaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAAAGGGGAATTGTTGTGtgtc                                            >  2:711111/1‑148 (MQ=255)
                                                                                                  aaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAACACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtctc                                            <  1:2941036/148‑1 (MQ=255)
                                                                                                  aaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTAACGTTAACCACATTTATCACGACCATACCAATAACCCGGAAGATTGCCGTAACTGGGGATTGTTGTGtgtc                                            >  1:3283232/1‑148 (MQ=255)
                                                                                                   aaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTctct                                           >  2:1153930/1‑148 (MQ=255)
                                                                                                     aGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTTTTCCCGCTAGTTTTTTTATTACAACCTGCCTGAACATTACGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACAGGGAATTGTTTTGTCTCTAt                                         >  1:2913737/1‑148 (MQ=255)
                                                                                                     aGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCAAGACCATACCAATAAACCGGAAGATGGCAGGAACTGGGGATTTTTGTGTGTCTAt                                         >  1:2131521/1‑148 (MQ=255)
                                                                                                        aTTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGc                                      >  2:3441633/1‑148 (MQ=255)
                                                                                                        aTTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGc                                      >  2:575181/1‑148 (MQ=255)
                                                                                                         ttAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGcc                                     >  2:879658/1‑148 (MQ=255)
                                                                                                               aTCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATc                               <  2:2691613/148‑1 (MQ=255)
                                                                                                               aTCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATc                               <  2:854272/148‑1 (MQ=255)
                                                                                                                 cTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTTATAACGACCATAACAATAAACCCGAACATGGCAGTAACTGGGAATTGTTTTGTCTCTATTTGCACGATCAt                             >  1:2792581/1‑148 (MQ=255)
                                                                                                                 cTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGACGATGGCCGTAACTGGGAATTGTTGTGTCTGTATTTCCACGATCAt                             >  1:1122295/1‑148 (MQ=255)
                                                                                                                   aTCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGa                           <  1:1434212/148‑1 (MQ=255)
                                                                                                                   aTCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGa                           >  2:1065941/1‑148 (MQ=255)
                                                                                                                   aTCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGa                           <  1:1106046/148‑1 (MQ=255)
                                                                                                                   aTCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGa                           <  1:2425927/148‑1 (MQ=255)
                                                                                                                   aTCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAAACAGCGTGAACATACCGTTCACCACATTGATCACGAACATAACAATAACCCGGAACATGGCAGTAACAGGGAATTGTTGTGTCTCAATTGCCAAGATCAtta                           >  1:2332779/1‑146 (MQ=255)
                                                                                                                     ccGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGc                         <  2:3468805/148‑1 (MQ=255)
                                                                                                                     ccGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGc                         <  1:3456518/148‑1 (MQ=255)
                                                                                                                      cGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCa                        <  2:2273040/148‑1 (MQ=255)
                                                                                                                        tGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCAtt                      <  1:1621280/148‑1 (MQ=255)
                                                                                                                        tGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAAATTACCCTTCACCACATTTATAACGACCAAAACAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTTTTTGTCTATTTCCACCATCATGAGCAtt                      >  1:176059/1‑148 (MQ=255)
                                                                                                                         gggTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTc                     >  1:3166172/1‑148 (MQ=255)
                                                                                                                          ggTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCg                    >  2:432250/1‑148 (MQ=255)
                                                                                                                               gCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAata               <  2:3356538/148‑1 (MQ=255)
                                                                                                                                   tCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATAcc           >  2:3299984/1‑148 (MQ=255)
                                                                                                                                      cTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGaa        <  2:2595240/148‑1 (MQ=255)
                                                                                                                                           cccGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGa   >  2:2833458/1‑148 (MQ=255)
                                                                                                                                            ccGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAt  >  1:2898921/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT  >  CP009273/426013‑426300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTAT  >  CP009273/426013‑426306
                                                                                                                                                |                                                                                                                                                     
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTT                                                                                                                                                  <  SRR19973065.2529008/150‑1 (MQ=60)
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTT                                                                                                                                                  <  SRR19973065.551379/150‑1 (MQ=60)
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTT                                                                                                                                                  >  SRR19973065.2785129/1‑150 (MQ=60)
  CTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGT                                                                                                                                                >  SRR19973065.2740013/1‑150 (MQ=60)
       GAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATT                                                                                                                                           <  SRR19973065.3519693/150‑1 (MQ=60)
       GAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATT                                                                                                                                           <  SRR19973065.3519702/150‑1 (MQ=60)
       GAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATT                                                                                                                                           <  SRR19973065.3519695/150‑1 (MQ=60)
          ACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCA                                                                                                                                        >  SRR19973065.2069913/1‑150 (MQ=60)
                CGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTTTTACAACCTGC                                                                                                                                  >  SRR19973065.382583/1‑150 (MQ=60)
                   AACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTG                                                                                                                               >  SRR19973065.1340693/1‑150 (MQ=60)
                   AACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTG                                                                                                                               >  SRR19973065.1846316/1‑150 (MQ=60)
                      CCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAAC                                                                                                                            >  SRR19973065.3452919/1‑150 (MQ=60)
                      CCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGGTTGTTTTTTCCAACCTGCGTGTAC                                                                                                                            >  SRR19973065.3452015/1‑150 (MQ=60)
                      CCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCGGTTGCCGCGAGTTTGGTTATTGCAACCTGCGTGAAC                                                                                                                            >  SRR19973065.1563099/1‑150 (MQ=60)
                         ACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTA                                                                                                                         <  SRR19973065.500274/150‑1 (MQ=60)
                             TTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGGTTGTTTATTCCAACCTGCGTGAACGTACCGT                                                                                                                     >  SRR19973065.52185/1‑150 (MQ=60)
                             TTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCAATAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCACGCGAGTTGGTTTATTCaaaccacagtgaactaaccga                                                                                                                     >  SRR19973065.1842263/1‑129 (MQ=60)
                                    AGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTGCAACCTGCGTGAACTTACCGTTAACCAC                                                                                                              >  SRR19973065.690624/1‑150 (MQ=60)
                                    AGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTACCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAACGTTCACCAC                                                                                                              >  SRR19973065.691321/1‑150 (MQ=60)
                                        AACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTG                                                                                                          <  SRR19973065.1041573/150‑1 (MQ=60)
                                         ACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCAGCGTGAACGTACCGTTCACCACAATGA                                                                                                         >  SRR19973065.812581/1‑150 (MQ=60)
                                            TTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCA                                                                                                      <  SRR19973065.3393194/150‑1 (MQ=60)
                                            TTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCA                                                                                                      <  SRR19973065.2720889/150‑1 (MQ=60)
                                            TTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCA                                                                                                      <  SRR19973065.3393872/150‑1 (MQ=60)
                                                ATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCCTTCACCACATTGATCACGAC                                                                                                  >  SRR19973065.192808/1‑150 (MQ=60)
                                                ATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAAACTGCGTGAACTTACCGTTAACCACCATGATCACGAC                                                                                                  >  SRR19973065.192827/1‑150 (MQ=60)
                                                 TGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACC                                                                                                 >  SRR19973065.2352243/1‑150 (MQ=60)
                                                     TATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTTAACTTACCGTTCACCACATTGATCACGACCATAC                                                                                             <  SRR19973065.1554081/150‑1 (MQ=60)
                                                     TATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATAC                                                                                             <  SRR19973065.1606413/150‑1 (MQ=60)
                                                     TATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATAC                                                                                             <  SRR19973065.1606388/150‑1 (MQ=60)
                                                      ATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTTATCACGACCATAAC                                                                                            >  SRR19973065.859797/1‑150 (MQ=60)
                                                      ATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACATACCGTTCACCACATTGATCACGACCAaaac                                                                                            >  SRR19973065.859819/1‑146 (MQ=60)
                                                      ATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTACCGCTAGTTTTTTTATTCCAACCAGCGTGAACTTACCGTTCACCACAATGATCACGACCAAACC                                                                                            >  SRR19973065.859815/1‑150 (MQ=60)
                                                         ATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACAATTATAACGACCAAACCAAT                                                                                         >  SRR19973065.629060/1‑150 (MQ=60)
                                                          TCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATA                                                                                        <  SRR19973065.1951533/150‑1 (MQ=60)
                                                            CCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGGGAACTTACCGTTCACCACAATGATCACGACCATACCAATAAC                                                                                      >  SRR19973065.2954706/1‑150 (MQ=60)
                                                             CAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTTGTTTGTTTATTACAACCTGCGTGAACTTACCGGTCACCACATTGATCACGCCCATTCCAATAACC                                                                                     >  SRR19973065.183001/1‑150 (MQ=60)
                                                               AAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCG                                                                                   <  SRR19973065.2076502/150‑1 (MQ=60)
                                                               AAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCG                                                                                   <  SRR19973065.2850960/150‑1 (MQ=60)
                                                                AAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGG                                                                                  >  SRR19973065.3142640/1‑150 (MQ=60)
                                                                         CGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCA                                                                         <  SRR19973065.96408/150‑1 (MQ=60)
                                                                             GTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAACTTACCGTTCACCACAATGATACCGACCATACCAAAAACCCGGAAGATGGCAGTAA                                                                     >  SRR19973065.1319324/1‑150 (MQ=60)
                                                                              TTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAAC                                                                    <  SRR19973065.2173279/150‑1 (MQ=60)
                                                                                  AAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGG                                                                <  SRR19973065.2801171/150‑1 (MQ=60)
                                                                                      CGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATT                                                            <  SRR19973065.3124213/150‑1 (MQ=60)
                                                                                      CGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCCACCTGCGTGAACTTACCGTTCACCACATTTATCACGACCAAAACAATAAACCGGAAGAAGGCAGTAACTGGGAATT                                                            >  SRR19973065.1669339/1‑150 (MQ=60)
                                                                                         CTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTT                                                         <  SRR19973065.1167300/150‑1 (MQ=60)
                                                                                          TATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTG                                                        >  SRR19973065.1171520/1‑150 (MQ=60)
                                                                                          TATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCCCTCGGTTGTTTTTTACAAACAGCGTGGACGTACCGTTCACCACAGTTATCACGACCAAAACAATAACCCCGAACATGGCAGTtacagggactttttt                                                        >  SRR19973065.2088374/1‑135 (MQ=49)
                                                                                           ATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGT                                                       <  SRR19973065.15452/150‑1 (MQ=60)
                                                                                           ATCGAGAAAAAGCTTTAAAAATCTATCCGTGGGTCTGCGGTCTCTGTTCCCGCTAGTTTTTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGT                                                       <  SRR19973065.16639/150‑1 (MQ=60)
                                                                                            TCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTG                                                      <  SRR19973065.1320451/150‑1 (MQ=60)
                                                                                            TCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTG                                                      <  SRR19973065.2915253/150‑1 (MQ=60)
                                                                                               CGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCT                                                   <  SRR19973065.2511711/150‑1 (MQ=60)
                                                                                               CGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCT                                                   <  SRR19973065.2906971/150‑1 (MQ=60)
                                                                                               CGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCGGCGTGAACTTACCGTTCACCACAATGATCACGACCATACCAAAAACCCGGCAGATGGCCGTAACAGGGCATTGTTGTGTCT                                                   >  SRR19973065.171310/1‑150 (MQ=60)
                                                                                               CGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCT                                                   >  SRR19973065.1957192/1‑150 (MQ=60)
                                                                                                GAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTC                                                  <  SRR19973065.3283073/150‑1 (MQ=60)
                                                                                                GAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTAACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTGTC                                                  >  SRR19973065.2547222/1‑150 (MQ=60)
                                                                                                GAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTAACGTTAACCACATTTATCACGACCATACCAATAACCCGGAAGATTGCCGTAACTGGGGATTGTTGTGTGTC                                                  >  SRR19973065.3303193/1‑150 (MQ=60)
                                                                                                 AAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCT                                                 <  SRR19973065.2564571/150‑1 (MQ=60)
                                                                                                 AAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCT                                                 <  SRR19973065.2566497/150‑1 (MQ=60)
                                                                                                  AAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTA                                                <  SRR19973065.720994/150‑1 (MQ=60)
                                                                                                  AAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGAACACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTA                                                <  SRR19973065.2958890/150‑1 (MQ=60)
                                                                                                   AAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTTTTCCCGCTAGTTTTTTTATTACAACCTGCCTGAACATTACGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACAGGGAATTGTTTTGTCTCTAT                                               >  SRR19973065.2931465/1‑150 (MQ=60)
                                                                                                   AAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACCTTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTAT                                               >  SRR19973065.2929620/1‑150 (MQ=60)
                                                                                                   AAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCAAGACCATACCAATAAACCGGAAGATGGCAGGAACTGGGGATTTTTGTGTGTCTAT                                               >  SRR19973065.2145236/1‑150 (MQ=60)
                                                                                                   AAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTACAACCAGCGTGAACATAACGTTCACCCCAATTATCACGACCATACCAATAAACCGGAAGATGGCACTAACTGGGAATTGTTTTGTCTCTAT                                               >  SRR19973065.2145353/1‑150 (MQ=60)
                                                                                                       CATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTtttttttgcaaccagcgggtacataccggtcaccacaatgataacgacccaaacaaaaaacccgaagatggccgttaatgggtatttttttgtgtgtattgac                                           >  SRR19973065.337870/1‑47 (MQ=60)
                                                                                                       CATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATTATAACCACCATACCAAAAACCCCGAAGATGGCAGTTACTGGGGAGTGTTTTGTATCTATTGGC                                           >  SRR19973065.459318/1‑150 (MQ=60)
                                                                                                         TTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCAC                                         <  SRR19973065.931653/150‑1 (MQ=60)
                                                                                                           AAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGA                                       >  SRR19973065.1768119/1‑150 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCTGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAT                                   >  SRR19973065.2300833/1‑150 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAT                                   <  SRR19973065.1484067/150‑1 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAT                                   >  SRR19973065.2890368/1‑150 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAT                                   <  SRR19973065.677403/150‑1 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTTATAACGACCATAACAATAAACCCGAACATGGCAGTAACTGGGAATTGTTTTGTCTCTATTTGCACGATCAT                                   >  SRR19973065.2809891/1‑150 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGACGATGGCCGTAACTGGGAATTGTTGTGTCTGTATTTCCACGATCAT                                   >  SRR19973065.1129607/1‑150 (MQ=60)
                                                                                                                TCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATG                                  <  SRR19973065.99244/150‑1 (MQ=60)
                                                                                                                 CTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGA                                 <  SRR19973065.1115960/150‑1 (MQ=60)
                                                                                                                 CTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAAACAGCGTGAACATACCGTTCACCACATTGATCACGAACATAACAATAACCCGGAACATGGCAGTAACAGGGAATTGTTGTGTCTCAATTGCCAAGATCATTA                                 >  SRR19973065.2347358/1‑150 (MQ=60)
                                                                                                                 CTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAAACCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGA                                 >  SRR19973065.569242/1‑150 (MQ=60)
                                                                                                                   ATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGC                               <  SRR19973065.1113295/150‑1 (MQ=60)
                                                                                                                   ATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGC                               <  SRR19973065.1443528/150‑1 (MQ=60)
                                                                                                                   ATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGC                               <  SRR19973065.2441235/150‑1 (MQ=60)
                                                                                                                   ATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCCCTATTGCCACGATCATGAGC                               <  SRR19973065.620410/150‑1 (MQ=60)
                                                                                                                     CCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCAT                             <  SRR19973065.3477584/150‑1 (MQ=60)
                                                                                                                      CGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAAATTACCCTTCACCACATTTATAACGACCAAAACAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTTTTTGTCTATTTCCACCATCATGAGCATT                            >  SRR19973065.177455/1‑150 (MQ=60)
                                                                                                                      CGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTACAAACAGCGTGAACATACCGTTCACCACAATTATCACGACCATACCAAAAACCCGGAAGATGGCCGTAACGGGGAATTGTTGTGTCTCTATTTCCACGATCATGAGCATT                            >  SRR19973065.177477/1‑150 (MQ=60)
                                                                                                                       GTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTC                           >  SRR19973065.3185351/1‑150 (MQ=60)
                                                                                                                        TGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCG                          <  SRR19973065.1632145/150‑1 (MQ=60)
                                                                                                                                 GGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACC                 <  SRR19973065.1607480/150‑1 (MQ=60)
                                                                                                                                 GGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACC                 <  SRR19973065.1348726/150‑1 (MQ=60)
                                                                                                                                          TCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT        >  SRR19973065.2916610/1‑150 (MQ=60)
                                                                                                                                          TCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT        <  SRR19973065.3432070/150‑1 (MQ=60)
                                                                                                                                          TCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT        >  SRR19973065.2840462/1‑150 (MQ=60)
                                                                                                                                           CCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATC       <  SRR19973065.1678253/150‑1 (MQ=60)
                                                                                                                                           CCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATC       <  SRR19973065.122409/150‑1 (MQ=60)
                                                                                                                                           CCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATC       <  SRR19973065.729495/150‑1 (MQ=60)
                                                                                                                                             CGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAG     >  SRR19973065.321895/1‑150 (MQ=60)
                                                                                                                                               CTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTA   >  SRR19973065.3286850/1‑150 (MQ=60)
                                                                                                                                                TAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTAT  >  SRR19973065.2649750/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGTAT  >  CP009273/426013‑426306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑