Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 503,944 G→T 100% E91* (GAA→TAA)  ybaQ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273503,9440GT94.4% 400.2 / ‑0.8 163E91* (GAA→TAA) ybaQputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (2/5);  major base T (63/79);  minor base A (14/0);  total (79/84)
Fisher's exact test for biased strand distribution p-value = 2.64e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCC  >  CP009273/503799‑504088
                                                                                                                                                 |                                                                                                                                                
tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGgaataa                                                                                                                                                <  1:3177879/148‑1 (MQ=255)
tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGgaataa                                                                                                                                                <  1:562407/148‑1 (MQ=255)
  cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAACCTCCAGGCGGCGGTTGATATTTGGGTAGTTGTAAAAAACATTCGCACCCAGGAATAAtt                                                                                                                                              >  1:2372385/1‑148 (MQ=255)
  cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAAtt                                                                                                                                              <  1:3005233/148‑1 (MQ=255)
  cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAAtt                                                                                                                                              >  1:2372424/1‑148 (MQ=255)
    cTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGATGTTTTGGGAGTTGTAAAAAAAATGCGCAACCACGAAAAATTgg                                                                                                                                            >  2:1320104/1‑148 (MQ=255)
    cTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGGTGATCTTTGGGAAGTTGTAAAAAACATGCGCACCCAGGAATAATTgg                                                                                                                                            >  1:2013524/1‑148 (MQ=255)
    cTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACTACAGGCGGCGGTTGTTGTTTTGGTAGTTGTAAACAACATGCGCACCCAGGAAAAAtttg                                                                                                                                            >  1:880221/1‑146 (MQ=255)
    cTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTgg                                                                                                                                            <  2:2599228/148‑1 (MQ=255)
      gATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGa                                                                                                                                          <  1:1257405/148‑1 (MQ=255)
        tCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAACCTCCACGCGGCGGGTTTTATTTGGGAAGTTGTAAAAAAAAATCCCACCCACGCAAAATTGGGACg                                                                                                                                        >  2:1209373/1‑148 (MQ=255)
        tCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAAACTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAAAAACATGCGCACCCACGAATAATTGGGACg                                                                                                                                        >  2:2274411/1‑148 (MQ=255)
          aaTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGa                                                                                                                                      <  1:1349088/148‑1 (MQ=255)
            tAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGAtt                                                                                                                                    >  2:2325854/1‑148 (MQ=255)
            tAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCGAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGAtt                                                                                                                                    >  2:2808119/1‑148 (MQ=255)
                aaTCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAAACTCCAGGCGGCGGTTGATCTTTGGGAAGTTTTAAACAAAATGCGCACCCAGGAATAATTGGGGCGGGTTTaaa                                                                                                                                >  1:2606465/1‑148 (MQ=255)
                aaTCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAGTTTTGGCTAAACCTCCAGGCGGCGGTTGTTCTTTGGGAAGTTGAAAAAAACAAGCGCACCCAGGACAAATTGGGTCGGATTGaaa                                                                                                                                >  1:2605980/1‑148 (MQ=255)
                 aTCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGGTTGAAAc                                                                                                                               >  2:3262645/1‑148 (MQ=255)
                  tCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAAACACCAGGCGGCGGGTGTTGTTTTGGTAGTTGTAAAAAAAATGCGCACCCCCGAAAAAATTGGTCGGATTGtaaaa                                                                                                                              >  1:823438/1‑146 (MQ=255)
                  tCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAGTTTTGGGTAAAACACCACGCGGGGGGTGGTGTTTTGGTAGTTGTAAACAACATTCGCACCCAGGAATAATTGGGGCGGCTTGTAACa                                                                                                                              >  1:823486/1‑148 (MQ=255)
                     tAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTg                                                                                                                           <  2:54035/148‑1 (MQ=255)
                      aaaCTCACTCCTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTgg                                                                                                                          <  2:659717/148‑1 (MQ=255)
                       aaCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCGAAACCTCCAGGCGGCGGGTGTTATTTGGGAAGTTGTAAAAAACAAGCGCACCCACGCATAATTTGGAGCGATTTAAAAAGTGGc                                                                                                                         >  1:1880463/1‑148 (MQ=255)
                            actactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAat                                                                                                                    <  1:3065168/148‑1 (MQ=255)
                                 tGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTg                                                                                                               <  1:1645710/148‑1 (MQ=255)
                                  gagaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCCGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTgg                                                                                                              >  2:2312329/1‑148 (MQ=255)
                                  gagaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAGTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAAAAACATGCGCACCCAGGAATAATTGGGACGGATTGAAAAAGTGGCTGAATATTTgg                                                                                                              >  2:3269353/1‑148 (MQ=255)
                                   agaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGc                                                                                                             <  1:2015412/148‑1 (MQ=255)
                                   agaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGc                                                                                                             <  1:547918/148‑1 (MQ=255)
                                    gaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAACCACCAGGCGGCGGTTGTTCTTTGGGAAGTTGTAAACAACATGCGCACCCCGGACAAATTGGGGCGGATTGTAACAGTGGCTGAATAttttgga                                                                                                            >  1:3452409/1‑144 (MQ=255)
                                    gaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTTATATTTGGGAAGTTGAAAACAAAATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCa                                                                                                            >  2:240546/1‑148 (MQ=255)
                                    gaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCa                                                                                                            <  2:192882/148‑1 (MQ=255)
                                       ggCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTACAGGCGGCGGTTGATCTTTGGGAACTTGAAAACAACATGCGCACCCAGGAAAAATTGGGCCGGATTGAAACACTGGCTGAATATTTTGCACGc                                                                                                         >  2:524485/1‑148 (MQ=255)
                                           tttCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCCGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACGCCGTg                                                                                                     >  2:1607732/1‑148 (MQ=255)
                                             tCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTTATCTTTGGGCAGTTGAAAACAACATGCGCACCCAGGAAAAATTGGGCCGGATTGAAACAGTGGCTGAATATTTGGGACGCCGTGaa                                                                                                   >  2:763979/1‑148 (MQ=255)
                                                 cTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGc                                                                                               <  1:1229164/148‑1 (MQ=255)
                                                  tGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCg                                                                                              <  2:1650012/148‑1 (MQ=255)
                                                  tGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCg                                                                                              <  1:437602/148‑1 (MQ=255)
                                                  tGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCg                                                                                              <  1:3350835/148‑1 (MQ=255)
                                                   ggCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTTGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGt                                                                                             >  2:1889348/1‑148 (MQ=255)
                                                    gCGAAAGTTTTTGATACCACAGTCGATTTTTGGGGAAAACACCAGGCGGCGGTTGATGTTTTGGAAGTTGTAAACAACAATCGCACCCGGGAATAATTGGGGGGGTTTGTAACAGTGGCTGAATATTTTGCACGCCGTGAAGAGGGTg                                                                                            >  1:1749551/1‑148 (MQ=255)
                                                     cGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGc                                                                                           <  1:3373012/148‑1 (MQ=255)
                                                      caaaGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGGTGATCTTTGGGAAGTTTGAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGTAACACTGGCTGAATATTTTGGACGCCGTGCAGAGCGTGcc                                                                                          >  2:1564206/2‑147 (MQ=255)
                                                        aaGTTTTTGATACCACAGTCGATTTTTTGCTAAAACTCCAGGCGGCGGGTGTTATTTGGGAAGTTGTAAAAAACATGCGCACCCACGGATAATTTGGTCGGATTGAAAAACTGGCGGAATATTTTGCACGCCCTGAAGTGCGTGCcaa                                                                                        >  1:1469247/1‑148 (MQ=255)
                                                              ttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGt                                                                                  <  2:556718/148‑1 (MQ=255)
                                                                gATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTcg                                                                                <  2:1955704/148‑1 (MQ=255)
                                                                       caGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGATCTTTTGGAAGTTGAAAAAAAAAAGCGCACCCACGAAAAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAgg                                                                         >  2:34794/1‑148 (MQ=255)
                                                                        aGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGc                                                                        <  1:3286435/148‑1 (MQ=255)
                                                                         gTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGcc                                                                       >  2:540873/1‑148 (MQ=255)
                                                                          tCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGCATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGGGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCa                                                                      >  1:1862297/1‑148 (MQ=255)
                                                                            gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAAGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaa                                                                    <  2:1090829/148‑1 (MQ=255)
                                                                              tttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                  <  2:1603718/148‑1 (MQ=255)
                                                                               ttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                 <  1:3425968/148‑1 (MQ=255)
                                                                               ttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                 <  2:1504516/148‑1 (MQ=255)
                                                                                  tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCg                                                              <  1:3232604/148‑1 (MQ=255)
                                                                                  tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCg                                                              <  1:1799198/148‑1 (MQ=255)
                                                                                  tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCg                                                              <  2:652774/148‑1 (MQ=255)
                                                                                   ggCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGc                                                             <  1:2527461/148‑1 (MQ=255)
                                                                                    gCTAAACCTCCAGGCGGCGGTTGATCTTTTGGAAGTTGAAAACAACATGCGCACCCAGGAAAAATTTGGTCGGATTTAAACAGGGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAATGTCGCGTAAGGCCAAAACATCGCt                                                            >  1:2408915/1‑148 (MQ=255)
                                                                                      tAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                          <  2:2450498/148‑1 (MQ=255)
                                                                                         aCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACTGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtc                                                       >  1:2957064/1‑148 (MQ=255)
                                                                                           cTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtctc                                                     >  2:3275886/1‑148 (MQ=255)
                                                                                            tCCAGGCGGCGGTTGATCTTTGGGTAGTTGAAAACAACATGCGCACCCAGGTATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCg                                                    <  2:2480470/148‑1 (MQ=255)
                                                                                              cAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCAGCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTa                                                  <  1:2488328/148‑1 (MQ=255)
                                                                                              cAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTa                                                  <  1:2488339/148‑1 (MQ=255)
                                                                                                ggcggcGGTTGATCTTTGGGAAGTTGAAAAAAACATGCGCACCCAGGAATAATTGGGACGGATTGTAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAgg                                                >  1:3013788/1‑148 (MQ=255)
                                                                                                     cggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGACTAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGa                                           <  2:2492770/148‑1 (MQ=255)
                                                                                                     cggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGa                                           >  2:1458726/1‑148 (MQ=255)
                                                                                                       gTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATa                                         <  2:3110381/148‑1 (MQ=255)
                                                                                                        ttGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                        <  1:2867498/148‑1 (MQ=255)
                                                                                                        ttGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                        <  1:2867531/148‑1 (MQ=255)
                                                                                                        gtGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                        >  1:1640794/2‑148 (MQ=255)
                                                                                                         tGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAg                                       >  2:1769985/1‑148 (MQ=255)
                                                                                                         tGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAg                                       >  1:1836689/1‑148 (MQ=255)
                                                                                                            tCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCg                                    <  1:2846169/148‑1 (MQ=255)
                                                                                                            tCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCg                                    <  1:1567119/148‑1 (MQ=255)
                                                                                                            tCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCg                                    >  2:1032331/1‑148 (MQ=255)
                                                                                                             cTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGt                                   >  1:2455660/1‑148 (MQ=255)
                                                                                                                  ggAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcg                              >  2:1929035/1‑148 (MQ=255)
                                                                                                                     aGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgccg                           >  2:268556/1‑148 (MQ=255)
                                                                                                                      gTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgccgc                          >  2:877739/1‑148 (MQ=255)
                                                                                                                      gTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgccgc                          >  1:1171442/1‑148 (MQ=255)
                                                                                                                      gTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgccgc                          >  1:2762408/1‑148 (MQ=255)
                                                                                                                       ttGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgca                         <  1:2823379/148‑1 (MQ=255)
                                                                                                                        tGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcat                        <  1:155118/148‑1 (MQ=255)
                                                                                                                        tGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcat                        <  1:2325854/148‑1 (MQ=255)
                                                                                                                           aaaCAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccg                     <  1:2209806/148‑1 (MQ=255)
                                                                                                                               aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                 <  1:1261536/148‑1 (MQ=255)
                                                                                                                               aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGAAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCTTTCACGCCgcatccggcat                 <  1:1540837/148‑1 (MQ=255)
                                                                                                                                acaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                >  1:2352392/1‑148 (MQ=255)
                                                                                                                                acaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                >  1:1691026/1‑148 (MQ=255)
                                                                                                                                acaTGCGCACCCAGGAAGAAGTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                <  2:1862297/148‑1 (MQ=255)
                                                                                                                                    gcgcACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAg            <  2:1718568/148‑1 (MQ=255)
                                                                                                                                    gcgcACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAg            <  1:1586507/148‑1 (MQ=255)
                                                                                                                                    gcgcACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAg            <  1:1586526/148‑1 (MQ=255)
                                                                                                                                         cccAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCa       >  2:3096704/1‑148 (MQ=255)
                                                                                                                                           cAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAtt     >  1:683166/1‑148 (MQ=255)
                                                                                                                                             ggAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGc   <  2:2764780/148‑1 (MQ=255)
                                                                                                                                              gaataaTTGGTACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGcc  >  1:2903931/1‑148 (MQ=255)
                                                                                                                                              gaataaTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGcc  >  1:1111447/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCC  >  CP009273/503799‑504088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  >  CP009273/503796‑504090
                                                                                                                                                     |                                                                                                                                                  
CAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATA                                                                                                                                                   <  SRR19973066.126123/150‑1 (MQ=60)
   TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAACCTCCA‑GGCGGCGGTTGATATTTGGGTAGTTGTAAAAAACATTCGCACCCAGGAATAATT                                                                                                                                                >  SRR19973066.2388416/1‑150 (MQ=60)
   TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCA‑GGCGGCGGTTTTTCTTTTGGAAGTTGAAAAAAACAAGCGCACCCAGGAATAATT                                                                                                                                                >  SRR19973066.2388420/1‑150 (MQ=60)
   TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATT                                                                                                                                                <  SRR19973066.3199027/150‑1 (MQ=60)
   TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATT                                                                                                                                                >  SRR19973066.2388456/1‑150 (MQ=60)
   TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATT                                                                                                                                                <  SRR19973066.566406/150‑1 (MQ=60)
   TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATT                                                                                                                                                <  SRR19973066.566415/150‑1 (MQ=60)
    GCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTG                                                                                                                                               <  SRR19973066.3003381/150‑1 (MQ=60)
     CACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCA‑GGCGGCGGGTGATCTTTGGGAAGTTGTAAAAAACATGCGCACCCAGGAATAATTGG                                                                                                                                              >  SRR19973066.2027629/1‑150 (MQ=60)
     CACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACTACA‑GGCGGCGGTTGTTGTTTTGGTAGTTGTAAACAACATGCGCACCCAGGAAAAATTTG                                                                                                                                              >  SRR19973066.886484/1‑150 (MQ=60)
     CACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGG                                                                                                                                              <  SRR19973066.3025220/150‑1 (MQ=60)
         GATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACG                                                                                                                                          <  SRR19973066.1266470/150‑1 (MQ=60)
            CAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGAT                                                                                                                                       <  SRR19973066.250373/150‑1 (MQ=60)
             AATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATT                                                                                                                                      <  SRR19973066.596120/150‑1 (MQ=60)
             AATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATT                                                                                                                                      <  SRR19973066.1358868/150‑1 (MQ=60)
              ATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTG                                                                                                                                     <  SRR19973066.1257822/150‑1 (MQ=60)
                 ACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAAACTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTTTAAACAAAATGCGCACCCAGGAATAATTGGGGCGGGTTTAAA                                                                                                                                  >  SRR19973066.2624090/1‑150 (MQ=60)
                 ACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAGTTTTGGCTAAACCTCCA‑GGCGGCGGTTGTTCTTTGGGAAGTTGAAAAAAACAAGCGCACCCAGGACAAATTGGGTCGGATTGAAA                                                                                                                                  >  SRR19973066.2623603/1‑150 (MQ=60)
                   AATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAAACACCA‑GGCGGGGGTTGTTGTTTGGGGAGTTTAAAAAAACAAGCGCACCCAGGCATAATTGGGGCGGGTTGTAACA                                                                                                                                >  SRR19973066.829500/1‑150 (MQ=60)
                   AATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAGTTTTGGGTAAAACACCA‑CGCGGGGGGTGGTGTTTTGGTAGTTGTAAACAACATTCGCACCCAGGAATAATTGGGGCGGCTTGTAACA                                                                                                                                >  SRR19973066.829499/1‑150 (MQ=60)
                         AAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCT                                                                                                                          <  SRR19973066.1572644/150‑1 (MQ=60)
                         AAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCT                                                                                                                          <  SRR19973066.3350853/150‑1 (MQ=60)
                               ACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATAT                                                                                                                    <  SRR19973066.3085669/150‑1 (MQ=60)
                                  ACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTG                                                                                                                 <  SRR19973066.1637103/150‑1 (MQ=60)
                                   CTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGG                                                                                                                <  SRR19973066.1329674/150‑1 (MQ=60)
                                    TGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGC                                                                                                               <  SRR19973066.1657693/150‑1 (MQ=60)
                                     GAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAACCACCA‑GGCGGCGGTTGTTCTTTGGGAAGTTGTAAACAACATGCGCACCCCGGACAAATTGGGGCGGATTGTAACAGTGGCTGAATATTTtgga                                                                                                              >  SRR19973066.3475493/1‑146 (MQ=60)
                                      AGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCAC                                                                                                             <  SRR19973066.2029525/150‑1 (MQ=60)
                                      AGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCAC                                                                                                             <  SRR19973066.551861/150‑1 (MQ=60)
                                                  GTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAAACTCCACGGGGGCGG‑GGATCTGTTGGAAGTTGTAAACAAAATGCCCACCCACCTAGAATAGGGAGGGTTTGAAACAGTGGGGGAATATTAGGCAAGCCGTGAAGAGC                                                                                                 >  SRR19973066.206193/1‑150 (MQ=24)
                                                    CTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGT                                                                                               <  SRR19973066.1237890/150‑1 (MQ=60)
                                                     TGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAAACTCCC‑GGCGGCGGGTGGTAGTTGGGGAGTTGAAAAAAACATGCGCCCCCAGGAATGAATGGGGCGGCTTTAAACAGTGGCTGCATATTTTGCACGgcctgtagggcggg                                                                                              >  SRR19973066.1762022/1‑136 (MQ=27)
                                                     TGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGGAAAACACCA‑GGCGGCGGTTGATGTTTTGGAAGTTGTAAACAACAATCGCACCCGGGAATAATTGGGGGGGTTTGTAACAGTGGCTGAATATTTTGCACGCCGTGAAGAGGGTG                                                                                              >  SRR19973066.1762138/1‑150 (MQ=60)
                                                     TGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTG                                                                                              <  SRR19973066.3373163/150‑1 (MQ=60)
                                                     TGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTG                                                                                              <  SRR19973066.3373177/150‑1 (MQ=60)
                                                     TGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTG                                                                                              <  SRR19973066.440988/150‑1 (MQ=60)
                                                     TGGCGAAAGTTTTTGAGACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTG                                                                                              <  SRR19973066.2247545/150‑1 (MQ=60)
                                                      GGCGAAAGTTTTTGATACCACAGTCGAGTTTTTGCTAAAACTCCA‑GGCGGCGGGTGGTCTTTTGGTAGTTTAAAACAACAATCGCACCCCCGAAAAATTGGGTCGGGTTGAAAAAGTGGCGGAAAATTTTGGACGCCCTGAAGAGCGTGG                                                                                             >  SRR19973066.3205669/1‑150 (MQ=29)
                                                        CGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAA                                                                                           <  SRR19973066.3395514/150‑1 (MQ=60)
                                                         GAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCA‑GGCGGCGGGTGGTCTTTGGGAAGTTGAAAAAAAAAAGCGCACCCCGGAATAATTTGGTCGGGTTGAAAAAGTGGCTGAATATTTTGCACGCCCTGAAGAGCGTGCAAA                                                                                          >  SRR19973066.2364142/1‑150 (MQ=60)
                                                         GAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACTCCA‑GGCGGCGGGTGTTATTTGGGAAGTTGTAAAAAACATGCGCACCCACGGATAATTTGGTCGGATTGAAAAACTGGCGGAATATTTTGCACGCCCTGAAGTGCGTGCCAA                                                                                          >  SRR19973066.1479610/1‑150 (MQ=48)
                                                             GTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAG                                                                                      <  SRR19973066.2864429/150‑1 (MQ=60)
                                                              TTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGG                                                                                     <  SRR19973066.933931/150‑1 (MQ=60)
                                                                TTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTC                                                                                   <  SRR19973066.1595937/150‑1 (MQ=60)
                                                                           AGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGCATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGGGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCA                                                                        >  SRR19973066.1875608/1‑150 (MQ=60)
                                                                           AGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCA                                                                        >  SRR19973066.1876305/1‑150 (MQ=60)
                                                                           AGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCA                                                                        <  SRR19973066.3308086/150‑1 (MQ=60)
                                                                              CGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAA                                                                     >  SRR19973066.3059760/1‑150 (MQ=60)
                                                                                  TTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATC                                                                 <  SRR19973066.3448878/150‑1 (MQ=60)
                                                                                     TGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTTGGAAGTTGAAAACAACATGCGCACCCAGGAAAAATTTGGTCGGATTTAAACAGGGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAATGTCGCGTAAGGCCAAAACATCGCT                                                              >  SRR19973066.2425222/1‑150 (MQ=60)
                                                                                     TGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCT                                                              >  SRR19973066.2425204/1‑150 (MQ=60)
                                                                                     TGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCT                                                              <  SRR19973066.1811986/150‑1 (MQ=60)
                                                                                     TGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCT                                                              <  SRR19973066.3253954/150‑1 (MQ=60)
                                                                                      GGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTT                                                             <  SRR19973066.2544648/150‑1 (MQ=60)
                                                                                          AAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACTGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATC                                                         >  SRR19973066.2976661/1‑150 (MQ=60)
                                                                                                 CA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCAGCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGG                                                  <  SRR19973066.2505309/150‑1 (MQ=60)
                                                                                                 CA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGG                                                  <  SRR19973066.2505319/150‑1 (MQ=60)
                                                                                                 CA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGG                                                  <  SRR19973066.2505320/150‑1 (MQ=60)
                                                                                                 CA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAAAAACATGCGCACCCAGGAATAATTGGGACGGATTGTAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGG                                                  >  SRR19973066.3033870/1‑150 (MQ=60)
                                                                                                         CGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATA                                           <  SRR19973066.1462751/150‑1 (MQ=60)
                                                                                                          GGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAA                                          >  SRR19973066.1815283/1‑150 (MQ=60)
                                                                                                          GGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAA                                          >  SRR19973066.1653415/1‑150 (MQ=60)
                                                                                                          GGGTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAA                                          >  SRR19973066.1652728/1‑150 (MQ=60)
                                                                                                           GTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAG                                         >  SRR19973066.1849810/1‑150 (MQ=60)
                                                                                                            TTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGG                                        <  SRR19973066.2886734/150‑1 (MQ=60)
                                                                                                            TTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGG                                        <  SRR19973066.2886767/150‑1 (MQ=60)
                                                                                                               ATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGT                                     >  SRR19973066.2472394/1‑150 (MQ=60)
                                                                                                                TCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTT                                    <  SRR19973066.1578407/150‑1 (MQ=60)
                                                                                                                TCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTT                                    <  SRR19973066.2865672/150‑1 (MQ=60)
                                                                                                                TCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTT                                    <  SRR19973066.2865292/150‑1 (MQ=60)
                                                                                                                        AAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGC                            >  SRR19973066.2781005/1‑150 (MQ=60)
                                                                                                                        AAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGC                            >  SRR19973066.1179714/1‑150 (MQ=60)
                                                                                                                           TTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATC                         <  SRR19973066.2842352/150‑1 (MQ=60)
                                                                                                                            TGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCC                        <  SRR19973066.2341736/150‑1 (MQ=60)
                                                                                                                            TGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCC                        <  SRR19973066.1806271/150‑1 (MQ=60)
                                                                                                                            TGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCC                        <  SRR19973066.3500887/150‑1 (MQ=60)
                                                                                                                            TGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCC                        <  SRR19973066.156557/150‑1 (MQ=60)
                                                                                                                             GAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCG                       <  SRR19973066.2118287/150‑1 (MQ=60)
                                                                                                                             GAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCG                       <  SRR19973066.310680/150‑1 (MQ=60)
                                                                                                                               AAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGC                     <  SRR19973066.2531590/150‑1 (MQ=60)
                                                                                                                               AAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGC                     <  SRR19973066.2225114/150‑1 (MQ=60)
                                                                                                                                  CAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATC                  >  SRR19973066.2368369/1‑150 (MQ=60)
                                                                                                                                  CAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATC                  >  SRR19973066.1703327/1‑150 (MQ=60)
                                                                                                                                   AACCTGCGCACCCAGGAATACTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCC                 <  SRR19973066.1551909/150‑1 (MQ=60)
                                                                                                                                   AACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCC                 <  SRR19973066.1270641/150‑1 (MQ=60)
                                                                                                                                   AACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGAAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCTTTCACGCCGCATCCGGCATCC                 <  SRR19973066.1551850/150‑1 (MQ=60)
                                                                                                                                    CCATGCGCACCCAGGAAGCATTGGGACGGATTGAAACAGTGGCTGAATATTTGCCACGCCGTGAACAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCG                <  SRR19973066.2755602/150‑1 (MQ=60)
                                                                                                                                    ACATGCGCACCCAGGAATACTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCG                <  SRR19973066.1724372/150‑1 (MQ=60)
                                                                                                                                      ATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAG              <  SRR19973066.1902822/150‑1 (MQ=60)
                                                                                                                                        GCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCA            <  SRR19973066.1597985/150‑1 (MQ=60)
                                                                                                                                        GCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCA            <  SRR19973066.1598004/150‑1 (MQ=60)
                                                                                                                                            ACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAT        <  SRR19973066.119855/150‑1 (MQ=60)
                                                                                                                                             CCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATT       >  SRR19973066.688186/1‑150 (MQ=60)
                                                                                                                                                AGGAATAATTGGTACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCC    >  SRR19973066.2923330/1‑150 (MQ=60)
                                                                                                                                                AGGAATAATTGGTACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAAGCCGCATCAGGCATCCGAGCTCCATTGCC    >  SRR19973066.2923314/1‑150 (MQ=60)
                                                                                                                                                AGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCC    >  SRR19973066.1119481/1‑150 (MQ=60)
                                                                                                                                                 GGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCT   >  SRR19973066.1903105/1‑150 (MQ=60)
                                                                                                                                                  GAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  <  SRR19973066.3284095/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
CAGTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCA‑GGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  >  CP009273/503796‑504090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑