Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 503,944 G→T E91* (GAA→TAA)  ybaQ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273503,9440GT95.4% 622.3 / ‑2.6 216E91* (GAA→TAA) ybaQputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (107/99);  minor base A (10/0);  total (117/99)
Fisher's exact test for biased strand distribution p-value = 2.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

GTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  >  CP009273/503798‑504090
                                                                                                                                                  |                                                                                                                                                  
 tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAAtt                                                                                                                                                <  2:72392/150‑1 (MQ=255)
 tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAAtt                                                                                                                                                <  2:2786093/150‑1 (MQ=255)
  gCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTg                                                                                                                                               >  2:2065436/1‑150 (MQ=255)
  gCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTGTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACACCATGCGCACCCAGGGATAATTg                                                                                                                                               >  2:3550567/1‑150 (MQ=255)
   cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTgg                                                                                                                                              >  2:1957870/1‑150 (MQ=255)
   cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTgg                                                                                                                                              >  1:2508328/1‑150 (MQ=255)
   cACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTgg                                                                                                                                              <  2:2192083/150‑1 (MQ=255)
      tGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGAc                                                                                                                                           >  1:1511577/1‑150 (MQ=255)
        aTCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACgg                                                                                                                                         <  2:2723919/150‑1 (MQ=255)
           aaTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAAGCGGCGGTTGATCTTTGGGAAATTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGAtt                                                                                                                                      >  1:1133349/1‑150 (MQ=255)
            aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTg                                                                                                                                     >  2:3241425/1‑150 (MQ=255)
            aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTg                                                                                                                                     <  1:3457908/150‑1 (MQ=255)
            aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTg                                                                                                                                     >  1:594953/1‑150 (MQ=255)
            aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGATTg                                                                                                                                     >  1:3045208/1‑150 (MQ=255)
                cAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTACAAGCGGCGGTTGATAATTGGGAAATTGAAAAAAACATGCGCACCCAGGAATAATTGGGAAGGATTTAAAc                                                                                                                                 >  1:1020660/1‑150 (MQ=255)
                 aaTCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACa                                                                                                                                <  2:3209685/150‑1 (MQ=255)
                   tcttAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGt                                                                                                                              <  2:2014861/147‑1 (MQ=255)
                      tAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGc                                                                                                                           >  2:1722476/1‑150 (MQ=255)
                      tAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATAATTTGCTAAAACACCAAGAAGCAGTTAATCATTATGAAATTTAAAACAACAAGCGCAACCCAGAATAATTTGTACGAAATTAAACAGTGGc                                                                                                                           >  1:3587644/1‑150 (MQ=255)
                       aaaCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACAACCAAGCGGCGGTTGATATTTTGGAAATTTAAAACAACAAGCGCACACAGGAATAATTTGGACGGATTGAAACAATAGCt                                                                                                                          >  1:2632323/1‑150 (MQ=255)
                       aaaCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCt                                                                                                                          <  1:3191035/150‑1 (MQ=255)
                           tCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAat                                                                                                                      >  1:1204747/1‑150 (MQ=255)
                                actGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAATTGCTGAATATTTg                                                                                                                 >  1:3635899/1‑150 (MQ=255)
                                  tGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGc                                                                                                               <  1:1610813/150‑1 (MQ=255)
                                  tGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGc                                                                                                               <  2:1897143/150‑1 (MQ=255)
                                     gaTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACAACATGAGGCGGTTGTTCTTTTGGAAGTTGAAAACAAAATGCGCACCCAGGAAAAAATGGGAAGGATTGAAACCGTGGCTGAATATTTGGCACg                                                                                                            >  1:866362/1‑150 (MQ=255)
                                          cATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTg                                                                                                       <  2:282463/150‑1 (MQ=255)
                                          cATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAAACTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTg                                                                                                       >  2:3346114/1‑150 (MQ=255)
                                           aTTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCATTTGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACGCCGTGa                                                                                                      >  1:3105882/1‑150 (MQ=255)
                                             ttCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAag                                                                                                    >  2:3616442/1‑150 (MQ=255)
                                                gTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGTTCTTTTTGAATTTGAAAAAAACCAGCGCACCCAAGAAAAATTGGGACGGATTGAAACAATTGCTGAATATTTTGCACGCCCTGAagata                                                                                                 >  1:2614488/1‑148 (MQ=255)
                                                  cTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTTATCTTTGGGAAATTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGt                                                                                               >  1:1702988/1‑150 (MQ=255)
                                                  cTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGt                                                                                               <  2:1377743/150‑1 (MQ=255)
                                                       gAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaaa                                                                                          >  2:1813904/1‑150 (MQ=255)
                                                         aaGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaaaaa                                                                                        <  2:2911992/150‑1 (MQ=255)
                                                          aGTTTTTGATACCACAGTCGATTTTTGGGTAAACCTCCAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATGCGCACCCACGAATAATTGGGACGGCTTGAAACAGAGGCGGAATATTTGGCACGCCGTGAAGAGCGTGCaaaaaa                                                                                       >  2:983100/1‑150 (MQ=255)
                                                            tttttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAgg                                                                                     <  1:2951743/150‑1 (MQ=255)
                                                             ttttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGt                                                                                    <  2:800849/150‑1 (MQ=255)
                                                              tttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTc                                                                                   <  1:2533340/150‑1 (MQ=255)
                                                                tGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTTAAAACAACATGCGCACCCAGGAAAAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACACCATGAAGAGTGTGCAAAAAATGTcgc                                                                                 >  1:2793092/1‑150 (MQ=255)
                                                                 gATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGATcgcg                                                                                <  2:952818/150‑1 (MQ=255)
                                                                 gATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTcgcg                                                                                >  2:671994/1‑150 (MQ=255)
                                                                   tACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTa                                                                              >  2:1562529/1‑150 (MQ=255)
                                                                   tACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTa                                                                              <  2:2840651/150‑1 (MQ=255)
                                                                    aCCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGGaa                                                                             >  2:3661110/1‑150 (MQ=255)
                                                                         aGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCa                                                                        >  1:3160584/1‑150 (MQ=255)
                                                                          gTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaa                                                                       <  1:3579709/150‑1 (MQ=255)
                                                                          gTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaa                                                                       >  1:184768/1‑150 (MQ=255)
                                                                           tCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaa                                                                      <  1:2755229/150‑1 (MQ=255)
                                                                            cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaaa                                                                     >  2:3216505/1‑150 (MQ=255)
                                                                             gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                    >  1:2215376/1‑150 (MQ=255)
                                                                             gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                    <  1:126525/150‑1 (MQ=255)
                                                                                  ttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGc                                                               >  2:2425821/1‑150 (MQ=255)
                                                                                   tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCTTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCt                                                              >  1:2706661/1‑150 (MQ=255)
                                                                                   tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCt                                                              >  1:2270138/1‑150 (MQ=255)
                                                                                     gCTAAACCTCCAGGCGGCTGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                            <  2:2067672/150‑1 (MQ=255)
                                                                                     gCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                            <  2:1133349/150‑1 (MQ=255)
                                                                                          aCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtctc                                                       <  2:2639972/150‑1 (MQ=255)
                                                                                          aCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtctc                                                       <  2:330392/150‑1 (MQ=255)
                                                                                            cTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGt                                                     >  2:1031555/1‑150 (MQ=255)
                                                                                             tCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAATCAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTa                                                    >  2:1023641/1‑150 (MQ=255)
                                                                                                aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                 >  2:2569452/1‑150 (MQ=255)
                                                                                                aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                 >  1:3098190/1‑150 (MQ=255)
                                                                                                aGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                                 >  2:1344610/1‑150 (MQ=255)
                                                                                                  gcggcgGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCg                                               <  1:2082513/150‑1 (MQ=255)
                                                                                                   cggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCgg                                              >  1:465418/1‑150 (MQ=255)
                                                                                                    ggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGa                                             >  2:529671/1‑150 (MQ=255)
                                                                                                    ggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGa                                             >  2:1193910/1‑150 (MQ=255)
                                                                                                       ggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                          >  1:2103500/1‑150 (MQ=255)
                                                                                                       ggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                          <  2:2603943/150‑1 (MQ=255)
                                                                                                          tGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGc                                       >  1:1752851/1‑150 (MQ=255)
                                                                                                                ttGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAc                                 <  1:2006060/150‑1 (MQ=255)
                                                                                                                ttGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAc                                 <  2:2618529/150‑1 (MQ=255)
                                                                                                                ttGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAc                                 <  1:2796328/150‑1 (MQ=255)
                                                                                                                ttGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAc                                 >  2:86763/1‑150 (MQ=255)
                                                                                                                 tGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcg                                >  1:1434432/1‑150 (MQ=255)
                                                                                                                 tGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcg                                <  1:3744346/150‑1 (MQ=255)
                                                                                                                   ggAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGTAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgcc                              <  2:3045208/150‑1 (MQ=255)
                                                                                                                         tGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatcc                        <  1:427159/150‑1 (MQ=255)
                                                                                                                         tGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatcc                        >  1:2345772/1‑150 (MQ=255)
                                                                                                                          gAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccg                       <  2:3587582/150‑1 (MQ=255)
                                                                                                                             aacaacCTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggca                    <  2:3635899/150‑1 (MQ=255)
                                                                                                                              acaacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                   >  1:390967/1‑150 (MQ=255)
                                                                                                                              acaacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                   >  1:391115/1‑150 (MQ=255)
                                                                                                                               caacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                  >  2:1239707/1‑150 (MQ=255)
                                                                                                                               caacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                  >  2:1878214/1‑150 (MQ=255)
                                                                                                                                aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc                 <  1:3303965/150‑1 (MQ=255)
                                                                                                                                aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc                 <  2:1749174/150‑1 (MQ=255)
                                                                                                                                aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc                 <  2:208403/150‑1 (MQ=255)
                                                                                                                                aacaTGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc                 <  2:547397/150‑1 (MQ=255)
                                                                                                                                    tGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGc             >  1:903380/1‑150 (MQ=255)
                                                                                                                                      cgcACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAc           >  1:1320118/1‑150 (MQ=255)
                                                                                                                                      cgcACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAc           >  1:1318815/1‑150 (MQ=255)
                                                                                                                                        cACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCa         >  2:3253967/1‑150 (MQ=255)
                                                                                                                                          cccAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAtt       <  2:3731010/150‑1 (MQ=255)
                                                                                                                                          cccAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAtt       >  1:2933792/1‑150 (MQ=255)
                                                                                                                                              ggAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCt   >  1:799049/1‑150 (MQ=255)
                                                                                                                                              ggAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCt   >  1:2323362/1‑150 (MQ=255)
                                                                                                                                              ggAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCt   >  2:3011598/1‑150 (MQ=255)
                                                                                                                                               gaataaTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTg  >  2:433713/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTG  >  CP009273/503798‑504090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCT  >  CP009273/503801‑504089
                                                                                                                                               |                                                                                                                                                 
CACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGG                                                                                                                                             >  SRR19973061.2514720/1‑150 (MQ=60)
CACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGG                                                                                                                                             >  SRR19973061.2515023/1‑150 (MQ=60)
 ACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGG                                                                                                                                            <  SRR19973061.2123630/150‑1 (MQ=60)
   TGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGAC                                                                                                                                          >  SRR19973061.1515361/1‑150 (MQ=60)
       CAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGAT                                                                                                                                      <  SRR19973061.1951440/150‑1 (MQ=60)
       CAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGAT                                                                                                                                      <  SRR19973061.2955698/150‑1 (MQ=60)
       CAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGAT                                                                                                                                      <  SRR19973061.1355395/150‑1 (MQ=60)
       CAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGAT                                                                                                                                      <  SRR19973061.281502/150‑1 (MQ=60)
        AATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAAGCGGCGGTTGATCTTTGGGAAATTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGATT                                                                                                                                     >  SRR19973061.1136239/1‑150 (MQ=60)
         ATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAAACTCCAGGCGGCGGTTGATATGTGGGAAGTTTAAAAAAAAATGCGCACCCAGGAAAAAATGGGACGGATTG                                                                                                                                    >  SRR19973061.596500/1‑150 (MQ=60)
         ATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTG                                                                                                                                    <  SRR19973061.3466774/150‑1 (MQ=60)
         ATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTG                                                                                                                                    >  SRR19973061.596503/1‑150 (MQ=60)
         ATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTG                                                                                                                                    <  SRR19973061.1874762/150‑1 (MQ=60)
         ATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGATTG                                                                                                                                    >  SRR19973061.3053080/1‑150 (MQ=60)
             CAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAATTGAAAACAACATTCGCACCCAAGAATAATTTGGACGGATTGAAAC                                                                                                                                >  SRR19973061.2638776/1‑150 (MQ=60)
             CAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATTCGCACCCAGGAAAAATTGGGACGGATTGAAAC                                                                                                                                >  SRR19973061.2637763/1‑150 (MQ=60)
             CAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTACAAGCGGCGGTTGATAATTGGGAAATTGAAAAAAACATGCGCACCCAGGAATAATTGGGAAGGATTTAAAC                                                                                                                                >  SRR19973061.1023259/1‑150 (MQ=60)
                   TAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCAAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGC                                                                                                                          >  SRR19973061.3596778/1‑150 (MQ=60)
                    AAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACAACCAAGCGGCGGTTGATATTTTGGAAATTTAAAACAACAAGCGCACACAGGAATAATTTGGACGGATTGAAACAATAGCT                                                                                                                         >  SRR19973061.2639087/1‑150 (MQ=60)
                    AAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCT                                                                                                                         <  SRR19973061.3199223/150‑1 (MQ=60)
                    AAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCT                                                                                                                         >  SRR19973061.1202484/1‑150 (MQ=60)
                       CTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAA                                                                                                                      >  SRR19973061.3740555/1‑150 (MQ=60)
                       CTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCAAAACCACCCGGCGGCGGTTTATCATTTGGAAATTGAAAACAAAAAGCGCACCCAGGAAAAATTTGGACGGATTGAAACAATGGCTGAA                                                                                                                      >  SRR19973061.332929/1‑150 (MQ=60)
                        TCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAAT                                                                                                                     >  SRR19973061.1207771/1‑150 (MQ=60)
                           CTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATT                                                                                                                  <  SRR19973061.2339366/150‑1 (MQ=60)
                             ACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGATATTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAATTGCTGAATATTTG                                                                                                                >  SRR19973061.3645192/1‑150 (MQ=60)
                               TGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGC                                                                                                              <  SRR19973061.1614826/150‑1 (MQ=60)
                                GAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCA                                                                                                             <  SRR19973061.209508/150‑1 (MQ=60)
                                  GATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACAACATGAGGCGGTTGTTCTTTTGGAAGTTGAAAACAAAATGCGCACCCAGGAAAAAATGGGAAGGATTGAAACCGTGGCTGAATATTTGGCACG                                                                                                           >  SRR19973061.868606/1‑150 (MQ=60)
                                     GGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCNG                                                                                                        <  SRR19973061.2971640/150‑1 (MQ=60)
                                       CATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGATAATTGGGAAGTTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGATTGAAACAATTGCTGAATATTTTGCACACCGTG                                                                                                      >  SRR19973061.464510/1‑150 (MQ=60)
                                        ATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGA                                                                                                     >  SRR19973061.3113871/1‑150 (MQ=60)
                                        ATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCATTTGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACGCCGTGA                                                                                                     >  SRR19973061.3113886/1‑150 (MQ=60)
                                        ATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCAAAAACACCAGGCGGCGGTTGATAATTGGGAAATTGAAAACAACAAGCGCACCCAAGAAAAATTTGGACGGATTGAAACAATTGCTGAAAATTTGGCACCCCATGA                                                                                                     >  SRR19973061.1619076/1‑150 (MQ=60)
                                             GTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGTTCTTTTTGAATTTGAAAAAAACCAGCGCACCCAAGAAAAATTGGGACGGATTGAAACAATTGCTGAATATTTTGCACGCCCTGAAGAta                                                                                                >  SRR19973061.2621200/1‑148 (MQ=60)
                                              TCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCG                                                                                               <  SRR19973061.3699564/150‑1 (MQ=60)
                                               CTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTTATCTTTGGGAAATTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAATGGCTGAATATTTTGCACGCCGTGAAGAGCGT                                                                                              >  SRR19973061.1707264/1‑150 (MQ=60)
                                                   CGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAA                                                                                          <  SRR19973061.2315473/150‑1 (MQ=60)
                                                     AAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGATATTTTGGAAGTTTAAAACAACATTCGCACCCAGGAATAATTGGGACGGATTGAAACAATGGCTGAATATTTTGCACGCCGTGAAGAGCGTGCAAAA                                                                                        >  SRR19973061.89489/1‑150 (MQ=60)
                                                        GTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAG                                                                                     <  SRR19973061.1726793/150‑1 (MQ=60)
                                                         TTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGG                                                                                    <  SRR19973061.2959354/150‑1 (MQ=60)
                                                           TTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTC                                                                                  <  SRR19973061.3150546/150‑1 (MQ=60)
                                                           TTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTC                                                                                  <  SRR19973061.2539792/150‑1 (MQ=60)
                                                           TTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTC                                                                                  <  SRR19973061.1244994/150‑1 (MQ=60)
                                                           TTTGATACCACAGTCGATTTTTGGCTAAAACTACATGCGGCGGTTGATCTTTGGGAAGTTTAAAACAACATTCGCACCCAGGAATAAATGGGACAGATTTAAACAATTGCTGAATATTTGtaacaccctgaaaatcatgcaaaaaaagtc                                                                                  >  SRR19973061.2689612/1‑120 (MQ=60)
                                                             TGATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGATCTTTTGGAAGTTGAAAACAACATGCGCACCCCGGAAAAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACGCCGTGAAGAGTGTGCAAAAAATGTCGC                                                                                >  SRR19973061.2799916/1‑150 (MQ=60)
                                                             TGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTTAAAACAACATGCGCACCCAGGAAAAATTGGGACGGATTGAAACAGTGGCTGAATATTTTGCACACCATGAAGAGTGTGCAAAAAATGTCGC                                                                                >  SRR19973061.2800268/1‑150 (MQ=60)
                                                             TGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTTAAAACAACATGCGCACCCAGGAAAAAATGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGTGTGCAAAAAATGTCGC                                                                                >  SRR19973061.2800881/1‑150 (MQ=60)
                                                              GATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCG                                                                               <  SRR19973061.3249765/150‑1 (MQ=60)
                                                               ATACCACAGTCGATTTTTTGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACAAGCGCACCCAGGAATAATTGGGACGGATTTAAACAATGGCTGAATATTTTGCACGCCGTGAATAGCGTGCAAAAAATGTCGCGT                                                                              >  SRR19973061.1519863/1‑150 (MQ=60)
                                                                      AGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCA                                                                       >  SRR19973061.3168704/1‑150 (MQ=60)
                                                                       GTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAA                                                                      >  SRR19973061.185329/1‑150 (MQ=60)
                                                                       GTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAA                                                                      <  SRR19973061.3588888/150‑1 (MQ=60)
                                                                        TCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAA                                                                     <  SRR19973061.2762324/150‑1 (MQ=60)
                                                                          GATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAC                                                                   <  SRR19973061.126931/150‑1 (MQ=60)
                                                                          GATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAC                                                                   >  SRR19973061.2221899/1‑150 (MQ=60)
                                                                          GATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAC                                                                   >  SRR19973061.2220940/1‑150 (MQ=60)
                                                                          GATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAC                                                                   <  SRR19973061.127603/150‑1 (MQ=60)
                                                                            TTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACAT                                                                 <  SRR19973061.310533/150‑1 (MQ=60)
                                                                              TTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCG                                                               <  SRR19973061.1826718/150‑1 (MQ=60)
                                                                                TGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCTTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCT                                                             >  SRR19973061.2713625/1‑150 (MQ=60)
                                                                                TGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCT                                                             >  SRR19973061.2712990/1‑150 (MQ=60)
                                                                                TGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCT                                                             <  SRR19973061.696384/150‑1 (MQ=60)
                                                                                TGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCT                                                             >  SRR19973061.2275861/1‑150 (MQ=60)
                                                                                   CTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCCGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCACAAAAGGTCGCGTAAGGCCAAAACATCGCTTCA                                                          >  SRR19973061.2478531/1‑150 (MQ=60)
                                                                                       ACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTC                                                      >  SRR19973061.3170253/1‑150 (MQ=60)
                                                                                             AGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGC                                                >  SRR19973061.3106172/1‑150 (MQ=60)
                                                                                               GCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCG                                              <  SRR19973061.2087737/150‑1 (MQ=60)
                                                                                                CGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGG                                             >  SRR19973061.2752658/1‑150 (MQ=60)
                                                                                                CGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGG                                             >  SRR19973061.466682/1‑150 (MQ=60)
                                                                                                   CGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATA                                          <  SRR19973061.387848/150‑1 (MQ=60)
                                                                                                   CGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATA                                          <  SRR19973061.2299333/150‑1 (MQ=60)
                                                                                                   CGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATA                                          >  SRR19973061.3235506/1‑150 (MQ=60)
                                                                                                    GGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGTACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAA                                         >  SRR19973061.1119753/1‑150 (MQ=60)
                                                                                                    GGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAA                                         >  SRR19973061.2108782/1‑150 (MQ=60)
                                                                                                      TTGATCTTGGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGG                                       <  SRR19973061.3629705/150‑1 (MQ=60)
                                                                                                       TGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGC                                      >  SRR19973061.1757249/1‑150 (MQ=60)
                                                                                                         ATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGT                                    >  SRR19973061.625724/1‑150 (MQ=60)
                                                                                                           CTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTC                                  <  SRR19973061.81811/150‑1 (MQ=60)
                                                                                                             TTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAC                                <  SRR19973061.2803514/150‑1 (MQ=60)
                                                                                                             TTGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAC                                <  SRR19973061.2011091/150‑1 (MQ=60)
                                                                                                              TGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACG                               >  SRR19973061.1438048/1‑150 (MQ=60)
                                                                                                              TGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACG                               >  SRR19973061.1438078/1‑150 (MQ=60)
                                                                                                              TGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACG                               <  SRR19973061.3753928/150‑1 (MQ=60)
                                                                                                              TGGGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACG                               >  SRR19973061.1438024/1‑150 (MQ=60)
                                                                                                                GGAAGTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCC                             >  SRR19973061.2614476/1‑150 (MQ=60)
                                                                                                                    GTTGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCAT                         <  SRR19973061.2011100/150‑1 (MQ=60)
                                                                                                                      TGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCC                       <  SRR19973061.428116/150‑1 (MQ=60)
                                                                                                                      TGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCC                       <  SRR19973061.428317/150‑1 (MQ=60)
                                                                                                                      TGAAAACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCC                       >  SRR19973061.2351729/1‑150 (MQ=60)
                                                                                                                           ACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAT                  >  SRR19973061.392036/1‑150 (MQ=60)
                                                                                                                           ACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAT                  >  SRR19973061.392043/1‑150 (MQ=60)
                                                                                                                           ACAACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAT                  >  SRR19973061.392184/1‑150 (MQ=60)
                                                                                                                             AACATGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCC                <  SRR19973061.3312480/150‑1 (MQ=60)
                                                                                                                                 TGCGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGC            >  SRR19973061.905701/1‑150 (MQ=60)
                                                                                                                                   CGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAC          >  SRR19973061.1323436/1‑150 (MQ=60)
                                                                                                                                   CGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAC          >  SRR19973061.1322815/1‑150 (MQ=60)
                                                                                                                                   CGCACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAC          >  SRR19973061.1322127/1‑150 (MQ=60)
                                                                                                                                       CCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATT      >  SRR19973061.2941338/1‑150 (MQ=60)
                                                                                                                                       CCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATT      >  SRR19973061.2941554/1‑150 (MQ=60)
                                                                                                                                           GGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCT  >  SRR19973061.2329258/1‑150 (MQ=60)
                                                                                                                                           GGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCT  >  SRR19973061.801143/1‑150 (MQ=60)
                                                                                                                                               |                                                                                                                                                 
CACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCT  >  CP009273/503801‑504089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑