Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 639,231 G→A 100% L65F (CTT→TTT)  rnk ← regulator of nucleoside diphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273639,2310GA99.0% 658.5 / ‑2.3 203L65F (CTT→TTT) rnkregulator of nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (79/122);  total (80/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 203 total aligned reads displayed.

CCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATC  >  CP009273/639087‑639375
                                                                                                                                                |                                                                                                                                                
ccGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAtt                                                                                                                                               >  2:2094325/1‑148 (MQ=255)
 cGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTg                                                                                                                                              <  1:3313691/148‑1 (MQ=255)
 cGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTg                                                                                                                                              <  2:1069576/148‑1 (MQ=255)
  ggAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGc                                                                                                                                             <  2:2592821/148‑1 (MQ=255)
   gAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCg                                                                                                                                            >  1:2950041/1‑148 (MQ=255)
     aGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGa                                                                                                                                          <  1:2056340/148‑1 (MQ=255)
     aGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACAACAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGa                                                                                                                                          <  1:257198/148‑1 (MQ=255)
      gTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                         <  1:2627438/148‑1 (MQ=255)
      gTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                         >  1:3042536/1‑148 (MQ=255)
         cccAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                      <  2:1260434/148‑1 (MQ=255)
            aGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAc                                                                                                                                   >  2:623535/1‑148 (MQ=255)
            aGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAc                                                                                                                                   >  1:1370964/1‑148 (MQ=255)
             gTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGTAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAcc                                                                                                                                  >  1:1420618/1‑148 (MQ=255)
              tGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAccc                                                                                                                                 <  1:2162830/148‑1 (MQ=255)
              tGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAccc                                                                                                                                 >  1:2393852/1‑148 (MQ=255)
              tGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAccc                                                                                                                                 >  1:1711784/1‑148 (MQ=255)
                aaTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCgg                                                                                                                               <  1:63852/148‑1 (MQ=255)
                aaTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCgg                                                                                                                               <  1:360364/148‑1 (MQ=255)
                aaTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCgg                                                                                                                               <  2:2357478/148‑1 (MQ=255)
                aaTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACAGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCgg                                                                                                                               <  1:1135989/148‑1 (MQ=255)
                 aTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGc                                                                                                                              >  2:2019805/1‑148 (MQ=255)
                   ggAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTg                                                                                                                            <  1:951413/148‑1 (MQ=255)
                    gAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGt                                                                                                                           <  2:1998269/148‑1 (MQ=255)
                     aaTCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGtt                                                                                                                          >  1:3174553/1‑148 (MQ=255)
                     aaTCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGtt                                                                                                                          >  1:1739663/1‑148 (MQ=255)
                       tCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCa                                                                                                                        <  2:2777000/148‑1 (MQ=255)
                        cGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAt                                                                                                                       <  1:1636957/148‑1 (MQ=255)
                             aCGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTca                                                                                                                  >  2:1585519/1‑148 (MQ=255)
                                   aGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgt                                                                                                            <  2:650177/148‑1 (MQ=255)
                                     ccccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcg                                                                                                          >  1:1625903/1‑148 (MQ=255)
                                       ccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCATCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCgtg                                                                                                        <  2:1759562/148‑1 (MQ=255)
                                       ccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCgtg                                                                                                        <  2:3028941/148‑1 (MQ=255)
                                       ccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCgtg                                                                                                        <  1:245599/148‑1 (MQ=255)
                                        cagcagTGCGGCACCAACCGGAGCCATAACGGAAAGCTGAGAATTGCTATCGGTCATTTTTTCCGGATACACCAGCGTGCGCACACGCACTTCGCCAACGCTAAAATTGCGGAATTTAACACGGCTGTTCATTGTCACCACGTCgtgt                                                                                                       >  1:2971262/1‑148 (MQ=255)
                                             gTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAt                                                                                                  <  2:568271/148‑1 (MQ=255)
                                              tGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtc                                                                                                 <  2:906490/148‑1 (MQ=255)
                                              tGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtc                                                                                                 <  1:749358/148‑1 (MQ=255)
                                               gCGGCACCTACCGGAGCCATAACGGAAATCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtct                                                                                                <  2:1646390/148‑1 (MQ=255)
                                               gCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtct                                                                                                <  1:893511/148‑1 (MQ=255)
                                                 ggCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATctct                                                                                              <  2:1868185/148‑1 (MQ=255)
                                                 ggCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATctct                                                                                              <  2:1938555/148‑1 (MQ=255)
                                                 ggCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATctct                                                                                              <  1:2748686/148‑1 (MQ=255)
                                                  gCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtctt                                                                                             <  1:1835909/148‑1 (MQ=255)
                                                    aCCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttct                                                                                           <  1:561641/148‑1 (MQ=255)
                                                      cTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTgg                                                                                         >  2:3457389/1‑148 (MQ=255)
                                                       tACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGc                                                                                        >  2:3184797/1‑148 (MQ=255)
                                                       tACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGc                                                                                        >  2:953147/1‑148 (MQ=255)
                                                        aCCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCg                                                                                       >  1:1966704/1‑148 (MQ=255)
                                                        aCCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCg                                                                                       >  2:3487214/1‑148 (MQ=255)
                                                          cGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGaa                                                                                     >  1:850358/1‑148 (MQ=255)
                                                            gAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAaca                                                                                   <  1:937537/148‑1 (MQ=255)
                                                               ccATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAt                                                                                >  2:2203393/1‑148 (MQ=255)
                                                                cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAtt                                                                               <  1:819107/148‑1 (MQ=255)
                                                                cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAtt                                                                               <  1:465976/148‑1 (MQ=255)
                                                                cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAtt                                                                               <  2:3154842/148‑1 (MQ=255)
                                                                cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAtt                                                                               <  1:2907456/148‑1 (MQ=255)
                                                                cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCATGTGGCATCTCTTCTGGCGAACACAtt                                                                               <  1:824157/148‑1 (MQ=255)
                                                                cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCATGTGGCATCTCTTCTGGCGAACACAtt                                                                               <  1:824128/148‑1 (MQ=255)
                                                                  tAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTg                                                                             >  1:2153191/1‑148 (MQ=255)
                                                                    aCGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTggg                                                                           >  2:2568524/1‑148 (MQ=255)
                                                                      ggAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGgcg                                                                         <  1:2919520/148‑1 (MQ=255)
                                                                       gAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGgcgc                                                                        >  2:365109/1‑148 (MQ=255)
                                                                           gCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATc                                                                    <  1:1767155/148‑1 (MQ=255)
                                                                                 tATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAActc                                                              <  2:1012983/148‑1 (MQ=255)
                                                                                 tATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAActc                                                              >  2:2809076/1‑148 (MQ=255)
                                                                                     gCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCg                                                          <  2:2393852/148‑1 (MQ=255)
                                                                                      cTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGt                                                         <  2:2628728/148‑1 (MQ=255)
                                                                                        aTCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGttt                                                       >  1:2695363/1‑148 (MQ=255)
                                                                                            gTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGTGCGATCCAACTCTGCGTTTAAcg                                                   >  2:2275719/1‑148 (MQ=255)
                                                                                            gTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAcg                                                   <  1:2848915/148‑1 (MQ=255)
                                                                                             tCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAcgc                                                  >  1:2555459/1‑148 (MQ=255)
                                                                                             tCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAcgc                                                  >  2:664356/1‑148 (MQ=255)
                                                                                               aTTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGt                                                >  2:1965348/1‑148 (MQ=255)
                                                                                               aTTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGt                                                <  2:787401/148‑1 (MQ=255)
                                                                                               aTTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGt                                                >  2:1965698/1‑148 (MQ=255)
                                                                                                    tGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCg                                           <  1:3457389/148‑1 (MQ=255)
                                                                                                        ggATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTg                                       >  2:2938812/1‑148 (MQ=255)
                                                                                                         gATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTgg                                      >  1:3551176/1‑148 (MQ=255)
                                                                                                            acacCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAGTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCaa                                   >  2:1933654/1‑148 (MQ=255)
                                                                                                              acCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAAc                                 <  1:199788/148‑1 (MQ=255)
                                                                                                                cAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCa                               >  2:645782/1‑148 (MQ=255)
                                                                                                                 aGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAg                              <  1:1279231/148‑1 (MQ=255)
                                                                                                                  gCGTTCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGc                             <  2:3124722/148‑1 (MQ=255)
                                                                                                                  gCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGc                             <  2:2709168/148‑1 (MQ=255)
                                                                                                                  gCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGc                             <  1:833429/148‑1 (MQ=255)
                                                                                                                      gcgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAg                         >  1:850135/1‑148 (MQ=255)
                                                                                                                         cacaCGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCg                      <  2:572311/148‑1 (MQ=255)
                                                                                                                           cacGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCggg                    <  2:2153191/148‑1 (MQ=255)
                                                                                                                           cacGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCggg                    <  2:1739049/148‑1 (MQ=255)
                                                                                                                            acGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGt                   <  2:2863557/148‑1 (MQ=255)
                                                                                                                              gCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTg                 <  2:2971262/148‑1 (MQ=255)
                                                                                                                              gCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTg                 <  1:3141432/148‑1 (MQ=255)
                                                                                                                                aCTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCt               <  2:3173840/148‑1 (MQ=255)
                                                                                                                                 cTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACTCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTc              >  1:91478/1‑148 (MQ=255)
                                                                                                                                 cTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTc              >  2:2476268/1‑148 (MQ=255)
                                                                                                                                        aTCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGa       <  2:2360243/148‑1 (MQ=255)
                                                                                                                                        aTCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGa       >  1:713992/1‑148 (MQ=255)
                                                                                                                                          cGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAat     >  1:3358729/1‑148 (MQ=255)
                                                                                                                                           gCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAata    <  2:1370964/148‑1 (MQ=255)
                                                                                                                                            cTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAatat   <  1:3090341/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATC  >  CP009273/639087‑639375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATG  >  CP009273/639084‑639379
                                                                                                                                                   |                                                                                                                                                    
CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAT                                                                                                                                                    <  SRR19973066.1082552/150‑1 (MQ=60)
CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAT                                                                                                                                                    <  SRR19973066.1620175/150‑1 (MQ=60)
 GCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATT                                                                                                                                                   <  SRR19973066.2876880/150‑1 (MQ=60)
    CGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCG                                                                                                                                                >  SRR19973066.2969615/1‑150 (MQ=60)
    CGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCG                                                                                                                                                >  SRR19973066.1365999/1‑150 (MQ=60)
    CGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCG                                                                                                                                                >  SRR19973066.2969798/1‑150 (MQ=60)
    CGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCG                                                                                                                                                <  SRR19973066.3335687/150‑1 (MQ=60)
       AAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAA                                                                                                                                             >  SRR19973066.3062866/1‑150 (MQ=60)
        AGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAT                                                                                                                                            >  SRR19973066.679138/1‑150 (MQ=60)
        AGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAT                                                                                                                                            <  SRR19973066.2070610/150‑1 (MQ=60)
        AGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACAACAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAT                                                                                                                                            <  SRR19973066.259145/150‑1 (MQ=60)
         GTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATT                                                                                                                                           <  SRR19973066.885864/150‑1 (MQ=60)
         GTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATT                                                                                                                                           <  SRR19973066.2645146/150‑1 (MQ=60)
             CCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAC                                                                                                                                       >  SRR19973066.1380883/1‑150 (MQ=60)
             CCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAC                                                                                                                                       <  SRR19973066.285471/150‑1 (MQ=60)
              CAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGTAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACC                                                                                                                                      >  SRR19973066.1430785/1‑150 (MQ=60)
               AGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCC                                                                                                                                     >  SRR19973066.1724200/1‑150 (MQ=60)
               AGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCC                                                                                                                                     >  SRR19973066.2409973/1‑150 (MQ=60)
                 TGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGG                                                                                                                                   <  SRR19973066.2177856/150‑1 (MQ=60)
                   AATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCT                                                                                                                                 <  SRR19973066.363177/150‑1 (MQ=60)
                   AATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCT                                                                                                                                 <  SRR19973066.64633/150‑1 (MQ=60)
                   AATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACAGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCT                                                                                                                                 <  SRR19973066.1144128/150‑1 (MQ=60)
                      GGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTT                                                                                                                              <  SRR19973066.958388/150‑1 (MQ=60)
                      GGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTT                                                                                                                              >  SRR19973066.3194974/1‑150 (MQ=60)
                      GGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTT                                                                                                                              >  SRR19973066.1751598/1‑150 (MQ=60)
                      GGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTT                                                                                                                              >  SRR19973066.3195689/1‑150 (MQ=60)
                      GGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTT                                                                                                                              >  SRR19973066.1752213/1‑150 (MQ=60)
                      GGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTT                                                                                                                              <  SRR19973066.958368/150‑1 (MQ=60)
                         ATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAT                                                                                                                           <  SRR19973066.2583663/150‑1 (MQ=60)
                           CGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTG                                                                                                                         <  SRR19973066.1648611/150‑1 (MQ=60)
                           CGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTG                                                                                                                         <  SRR19973066.1648856/150‑1 (MQ=60)
                            GCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGT                                                                                                                        >  SRR19973066.210647/1‑150 (MQ=60)
                                      AGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCG                                                                                                              >  SRR19973066.1637689/1‑150 (MQ=60)
                                      AGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCG                                                                                                              <  SRR19973066.933944/150‑1 (MQ=60)
                                       GCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGT                                                                                                             <  SRR19973066.1761129/150‑1 (MQ=60)
                                        CCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTG                                                                                                            >  SRR19973066.531334/1‑150 (MQ=60)
                                        CCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTG                                                                                                            <  SRR19973066.313000/150‑1 (MQ=60)
                                         CCCAGCAGTGCGGCACCAACCGGAGCCATAACGGAAAGCTGAGAATTGCTATCGGTCATTTTTTCCGGATACACCAGCGTGCGCACACGCACTTCGCCAACGCTAAAATTGCGGAATTTAACACGGCTGTTCATTGTCACCACGTCGTGT                                                                                                           >  SRR19973066.2990902/1‑150 (MQ=60)
                                          CCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTG                                                                                                          <  SRR19973066.247505/150‑1 (MQ=60)
                                          CCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTG                                                                                                          <  SRR19973066.247354/150‑1 (MQ=60)
                                            AGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGC                                                                                                        >  SRR19973066.1429326/1‑150 (MQ=60)
                                                 TGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTC                                                                                                   <  SRR19973066.3490/150‑1 (MQ=60)
                                                 TGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTC                                                                                                   <  SRR19973066.754917/150‑1 (MQ=60)
                                                 TGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTC                                                                                                   <  SRR19973066.2056582/150‑1 (MQ=60)
                                                  GCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCT                                                                                                  <  SRR19973066.900374/150‑1 (MQ=60)
                                                  GCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCT                                                                                                  <  SRR19973066.899832/150‑1 (MQ=60)
                                                    GGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTC                                                                                                <  SRR19973066.2767184/150‑1 (MQ=60)
                                                     GCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCT                                                                                               <  SRR19973066.1849023/150‑1 (MQ=60)
                                                       ACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGG                                                                                             <  SRR19973066.565648/150‑1 (MQ=60)
                                                        CCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGC                                                                                            <  SRR19973066.2400508/150‑1 (MQ=60)
                                                         CTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCG                                                                                           >  SRR19973066.1980600/1‑150 (MQ=60)
                                                         CTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCG                                                                                           >  SRR19973066.3343313/1‑150 (MQ=60)
                                                         CTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCG                                                                                           >  SRR19973066.793204/1‑150 (MQ=60)
                                                           ACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAA                                                                                         >  SRR19973066.1228703/1‑150 (MQ=60)
                                                           ACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAA                                                                                         >  SRR19973066.856509/1‑150 (MQ=60)
                                                           ACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAA                                                                                         >  SRR19973066.856507/1‑150 (MQ=60)
                                                           ACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAA                                                                                         >  SRR19973066.3017754/1‑150 (MQ=60)
                                                            CCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAAC                                                                                        <  SRR19973066.1531867/150‑1 (MQ=60)
                                                               GAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACA                                                                                     <  SRR19973066.60654/150‑1 (MQ=60)
                                                               GAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACA                                                                                     <  SRR19973066.944357/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.825096/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.469525/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 >  SRR19973066.2168210/1‑150 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 >  SRR19973066.2168140/1‑150 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.825132/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.2875171/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.2926867/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.2926878/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCATGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.830143/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCATGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.830172/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCATGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.830423/150‑1 (MQ=60)
                                                                   CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCATGTGGCATCTCTTCTGGCGAACACATTTG                                                                                 <  SRR19973066.830148/150‑1 (MQ=60)
                                                                     TAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGG                                                                               >  SRR19973066.854747/1‑150 (MQ=60)
                                                                       ACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCG                                                                             <  SRR19973066.951092/150‑1 (MQ=60)
                                                                         GGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCG                                                                           <  SRR19973066.2938985/150‑1 (MQ=60)
                                                                         GGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCG                                                                           <  SRR19973066.2938987/150‑1 (MQ=60)
                                                                              GCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCA                                                                      <  SRR19973066.3100896/150‑1 (MQ=60)
                                                                              GCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCA                                                                      <  SRR19973066.1779824/150‑1 (MQ=60)
                                                                                         CTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTT                                                           <  SRR19973066.2633937/150‑1 (MQ=60)
                                                                                         CTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTT                                                           >  SRR19973066.2713376/1‑150 (MQ=60)
                                                                                              GGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGC                                                      >  SRR19973066.2572812/1‑150 (MQ=60)
                                                                                               GTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCG                                                     <  SRR19973066.2868059/150‑1 (MQ=60)
                                                                                                  ATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCG                                                  <  SRR19973066.731429/150‑1 (MQ=60)
                                                                                                  ATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCG                                                  <  SRR19973066.2700633/150‑1 (MQ=60)
                                                                                                    TTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGC                                                <  SRR19973066.447624/150‑1 (MQ=60)
                                                                                                       TGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAT                                             <  SRR19973066.3480507/150‑1 (MQ=60)
                                                                                                       TGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAT                                             <  SRR19973066.3480504/150‑1 (MQ=60)
                                                                                                         CCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTG                                           <  SRR19973066.1892254/150‑1 (MQ=60)
                                                                                                          CGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGG                                          >  SRR19973066.3575020/1‑150 (MQ=60)
                                                                                                          CGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGG                                          >  SRR19973066.3574742/1‑150 (MQ=60)
                                                                                                                 ACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCA                                   <  SRR19973066.201485/150‑1 (MQ=60)
                                                                                                                 ACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCA                                   <  SRR19973066.201488/150‑1 (MQ=60)
                                                                                                                    AGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCA                                <  SRR19973066.1288471/150‑1 (MQ=60)
                                                                                                                     GCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCAT                               <  SRR19973066.839506/150‑1 (MQ=60)
                                                                                                                       GTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAG                             >  SRR19973066.856286/1‑150 (MQ=60)
                                                                                                                             ACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGT                       <  SRR19973066.3323944/150‑1 (MQ=60)
                                                                                                                                 GCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCT                   <  SRR19973066.3162392/150‑1 (MQ=60)
                                                                                                                                  CACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACTCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTC                  >  SRR19973066.92470/1‑150 (MQ=60)
                                                                                                                                    CTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCA                >  SRR19973066.2738760/1‑150 (MQ=60)
                                                                                                                                        GCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAG            >  SRR19973066.1658479/1‑150 (MQ=60)
                                                                                                                                         CCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGA           >  SRR19973066.719281/1‑150 (MQ=60)
                                                                                                                                          CATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAA          >  SRR19973066.2475284/1‑150 (MQ=60)
                                                                                                                                           ATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAAT         >  SRR19973066.3381126/1‑150 (MQ=60)
                                                                                                                                            TCGCTAAGATTGCGGAATTTACCCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCGTCTGGCGAACACATTGGGGCGCGATCCCACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATA        <  SRR19973066.349916/150‑1 (MQ=60)
                                                                                                                                              GCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATC      >  SRR19973066.1802506/1‑150 (MQ=60)
                                                                                                                                               CTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCG     <  SRR19973066.3110984/150‑1 (MQ=60)
                                                                                                                                                  AAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATG  >  SRR19973066.3272904/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATG  >  CP009273/639084‑639379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑