Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 639,231 G→A 89.8% L65F (CTT→TTT)  rnk ← regulator of nucleoside diphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273639,2310GA89.8% 799.4 / 64.3 255L65F (CTT→TTT) rnkregulator of nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (14/12);  new base A (109/120);  total (123/132)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGA  >  CP009273/639084‑639377
                                                                                                                                                   |                                                                                                                                                  
cgccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGAt                                                                                                                                                  >  2:3915415/1‑150 (MQ=255)
cgccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                  <  1:1368450/150‑1 (MQ=255)
cgccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                  >  2:1289815/1‑150 (MQ=255)
cgccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                  <  2:2289378/150‑1 (MQ=255)
cgccCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAt                                                                                                                                                  >  2:4200298/1‑150 (MQ=255)
    cGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCg                                                                                                                                              <  2:172595/150‑1 (MQ=255)
     ggAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCgg                                                                                                                                             >  1:3362218/1‑150 (MQ=255)
     ggAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCgg                                                                                                                                             >  1:592094/1‑150 (MQ=255)
     ggAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCgg                                                                                                                                             >  2:4117281/1‑150 (MQ=255)
       aaGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                           >  2:814243/1‑150 (MQ=255)
       aaGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                           >  1:2000306/1‑150 (MQ=255)
       aaGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                           >  2:2987694/1‑150 (MQ=255)
       aaGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                           >  2:3304012/1‑150 (MQ=255)
        aGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAt                                                                                                                                          <  1:3608486/150‑1 (MQ=255)
        aGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAt                                                                                                                                          >  2:3416945/1‑150 (MQ=255)
          ttCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                        <  1:100830/150‑1 (MQ=255)
          ttCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                        <  2:3526584/150‑1 (MQ=255)
          ttCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                        <  2:1373004/150‑1 (MQ=255)
           tCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTa                                                                                                                                       >  2:3605082/1‑150 (MQ=255)
                gTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCg                                                                                                                                  <  2:1332001/150‑1 (MQ=255)
                 tGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCgg                                                                                                                                 <  1:2168823/150‑1 (MQ=255)
                      ggAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGtt                                                                                                                            <  2:2509782/150‑1 (MQ=255)
                       gAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTc                                                                                                                           <  1:3607907/150‑1 (MQ=255)
                         aTCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAt                                                                                                                         <  1:3809043/150‑1 (MQ=255)
                              cAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTca                                                                                                                    >  2:441960/1‑150 (MQ=255)
                               aaCGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTcac                                                                                                                   >  1:3044484/1‑150 (MQ=255)
                               aaCGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTcac                                                                                                                   >  2:4032418/1‑150 (MQ=255)
                                 cgcgCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTcacca                                                                                                                 <  1:3638099/150‑1 (MQ=255)
                                   cgcAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcg                                                                                                               <  1:1543813/150‑1 (MQ=255)
                                      aGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcg                                                                                                            >  2:2600724/1‑150 (MQ=255)
                                       gCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcgt                                                                                                           >  2:1824998/1‑150 (MQ=255)
                                         cccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCgtgt                                                                                                         >  2:2027105/1‑150 (MQ=255)
                                           cagcagTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGACTTTAACCCGGCTGTTCATTGTCACCACGTCGTGTgg                                                                                                       >  2:1422051/1‑150 (MQ=255)
                                               agTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtc                                                                                                   <  2:1080161/150‑1 (MQ=255)
                                               agTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtc                                                                                                   >  2:146169/1‑150 (MQ=255)
                                                gTGCGGCACCGACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGAGCGCACCCGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtct                                                                                                  <  2:4128137/150‑1 (MQ=255)
                                                  gCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATctct                                                                                                <  1:346161/150‑1 (MQ=255)
                                                  gCGGCACCGACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtctcc                                                                                                <  1:2777232/150‑2 (MQ=255)
                                                    ggCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttc                                                                                              >  1:2378518/1‑150 (MQ=255)
                                                    ggCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttc                                                                                              <  1:2841049/150‑1 (MQ=255)
                                                    ggCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttc                                                                                              <  1:751286/150‑1 (MQ=255)
                                                     gCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtcttct                                                                                             <  2:1132349/150‑1 (MQ=255)
                                                        ccTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGc                                                                                          >  2:970756/1‑150 (MQ=255)
                                                        ccTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGc                                                                                          <  1:2620242/150‑1 (MQ=255)
                                                          tACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGa                                                                                        <  1:891252/150‑1 (MQ=255)
                                                           aCCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGaa                                                                                       <  1:1815151/150‑1 (MQ=255)
                                                                aGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAt                                                                                  <  1:2292227/150‑1 (MQ=255)
                                                                   cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTg                                                                               <  1:1058283/150‑1 (MQ=255)
                                                                   cATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTg                                                                               <  1:4306768/150‑1 (MQ=255)
                                                                     tAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCCTCTCTTCTGGCGAACACATTTggg                                                                             >  2:2272993/1‑150 (MQ=255)
                                                                         ggAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGcgcg                                                                         >  2:4307279/1‑150 (MQ=255)
                                                                           aaaGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGAt                                                                       >  2:649986/1‑150 (MQ=255)
                                                                           aaaGCTGAGTATTGCTATCGGTCATTTATGCCGGATACACCAGCGTGCGCACACGCACTACGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAAATCTTCTGGCGAACACAATTGGGCCCGaa                                                                       >  1:514861/1‑149 (MQ=255)
                                                                              gCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCa                                                                    <  2:739638/150‑1 (MQ=255)
                                                                               cTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCaa                                                                   >  2:600261/1‑150 (MQ=255)
                                                                                tGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAAc                                                                  <  2:2421590/150‑1 (MQ=255)
                                                                                 gAGTATTTCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACAAGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCTAACACATTTGGGCGCGATCCAAct                                                                 <  2:2605869/150‑1 (MQ=255)
                                                                                  aGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAActc                                                                >  2:841851/1‑150 (MQ=255)
                                                                                  aGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAActc                                                                >  2:2430639/1‑150 (MQ=255)
                                                                                   gTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAActct                                                               >  2:2762825/1‑150 (MQ=255)
                                                                                    tATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTg                                                              >  1:3176521/1‑150 (MQ=255)
                                                                                    tATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTg                                                              <  2:3103272/150‑1 (MQ=255)
                                                                                        gCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGtt                                                          >  2:2010151/1‑150 (MQ=255)
                                                                                         cTATCGGTCATTTTTGCCGGATACACCCGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGttt                                                         <  2:1831627/150‑1 (MQ=255)
                                                                                           aTCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGACCACATTTGGGCGCGATCCAACTCTGCGTTTaa                                                       >  1:2304305/1‑150 (MQ=255)
                                                                                             cGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAcg                                                     >  2:2084088/1‑150 (MQ=255)
                                                                                                 cATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTc                                                 <  2:1937750/150‑1 (MQ=255)
                                                                                                  aTTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCg                                                >  2:1678240/1‑150 (MQ=255)
                                                                                                    ttttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGc                                              <  2:1460836/150‑1 (MQ=255)
                                                                                                    ttttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGc                                              <  2:2813584/150‑1 (MQ=255)
                                                                                                     tttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCg                                             <  2:2457499/150‑1 (MQ=255)
                                                                                                      ttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGa                                            >  1:2991139/1‑150 (MQ=255)
                                                                                                      ttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGa                                            >  1:3724202/1‑150 (MQ=255)
                                                                                                          cGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTgg                                        >  2:3817909/1‑150 (MQ=255)
                                                                                                           ggATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTGTAACGCGTCGGCGATTGGc                                       >  1:3338179/1‑150 (MQ=255)
                                                                                                            gATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCa                                      <  2:409153/150‑1 (MQ=255)
                                                                                                             aTACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCaa                                     >  1:2366396/1‑150 (MQ=255)
                                                                                                              tACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCaaa                                    >  1:764761/1‑150 (MQ=255)
                                                                                                                cacCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAAcc                                  <  1:1978865/150‑1 (MQ=255)
                                                                                                                 acCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCa                                 >  1:315523/1‑150 (MQ=255)
                                                                                                                  ccAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAg                                <  1:3897775/150‑1 (MQ=255)
                                                                                                                    aGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTTGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCa                              >  1:904062/1‑150 (MQ=255)
                                                                                                                       gTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAg                           <  1:4250066/150‑1 (MQ=255)
                                                                                                                        tGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAgg                          <  2:528935/150‑1 (MQ=255)
                                                                                                                          cgcCCACGCACTTCGCCATCGCTAAAAGTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAGTGGCAAACCAGCCTAGGCg                        >  2:867869/1‑150 (MQ=255)
                                                                                                                          cgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCg                        >  2:3938578/1‑150 (MQ=255)
                                                                                                                            cacaCGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCggg                      <  1:1917096/150‑1 (MQ=255)
                                                                                                                             acacGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGt                     <  1:935174/150‑1 (MQ=255)
                                                                                                                              cacGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGtt                    <  1:4181296/150‑1 (MQ=255)
                                                                                                                              cacGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGtt                    <  1:4200298/150‑1 (MQ=255)
                                                                                                                              cacGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGtt                    <  1:1289815/150‑1 (MQ=255)
                                                                                                                                cGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCACACCAGCATAGGCGGGTTGc                  >  1:1599359/1‑150 (MQ=255)
                                                                                                                                 gCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCt                 >  1:2676161/1‑150 (MQ=255)
                                                                                                                                   aCTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTcc               <  1:2893948/150‑1 (MQ=255)
                                                                                                                                        gCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCcagcag          >  2:270872/1‑150 (MQ=255)
                                                                                                                                         ccATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGa         >  2:2146106/1‑150 (MQ=255)
                                                                                                                                             cGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAatat     >  1:689517/1‑150 (MQ=255)
                                                                                                                                              gCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATc    >  2:424543/1‑150 (MQ=255)
                                                                                                                                               cTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCg   <  2:287517/150‑1 (MQ=255)
                                                                                                                                                tAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCCGAATATCGa  >  2:3067526/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGA  >  CP009273/639084‑639377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGAT  >  CP009273/639082‑639378
                                                                                                                                                     |                                                                                                                                                   
GCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAA                                                                                                                                                     <  SRR19973079.2328582/150‑1 (MQ=60)
  CGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAAT                                                                                                                                                   <  SRR19973079.1376044/150‑1 (MQ=60)
       GGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGG                                                                                                                                              >  SRR19973079.3379517/1‑150 (MQ=60)
       GGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGG                                                                                                                                              >  SRR19973079.595557/1‑150 (MQ=60)
       GGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGG                                                                                                                                              <  SRR19973079.2388558/150‑1 (MQ=60)
       GGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGG                                                                                                                                              >  SRR19973079.3379527/1‑150 (MQ=60)
         AAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAA                                                                                                                                            >  SRR19973079.2010896/1‑150 (MQ=60)
          AGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAT                                                                                                                                           <  SRR19973079.1488569/150‑1 (MQ=60)
          AGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAT                                                                                                                                           <  SRR19973079.3626784/150‑1 (MQ=60)
           GGTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATT                                                                                                                                          <  SRR19973079.3091546/150‑1 (MQ=60)
            TTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTT                                                                                                                                         <  SRR19973079.101729/150‑1 (MQ=60)
            TTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTT                                                                                                                                         <  SRR19973079.1935730/150‑1 (MQ=60)
             TCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTA                                                                                                                                        <  SRR19973079.201184/150‑1 (MQ=60)
             TCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTA                                                                                                                                        <  SRR19973079.2722612/150‑1 (MQ=60)
               CCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAC                                                                                                                                      <  SRR19973079.3512350/150‑1 (MQ=60)
               CCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAC                                                                                                                                      <  SRR19973079.3425034/150‑1 (MQ=60)
                 AGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCC                                                                                                                                    >  SRR19973079.3839882/1‑150 (MQ=60)
                   TGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGG                                                                                                                                  <  SRR19973079.2179876/150‑1 (MQ=60)
                   TGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGG                                                                                                                                  <  SRR19973079.2179901/150‑1 (MQ=60)
                         GAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTC                                                                                                                            <  SRR19973079.3626213/150‑1 (MQ=60)
                         GAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTC                                                                                                                            <  SRR19973079.3626205/150‑1 (MQ=60)
                          AATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCA                                                                                                                           >  SRR19973079.287430/1‑150 (MQ=60)
                          AATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCA                                                                                                                           >  SRR19973079.288329/1‑150 (MQ=60)
                           ATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAT                                                                                                                          <  SRR19973079.3828537/150‑1 (MQ=60)
                           ATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAT                                                                                                                          <  SRR19973079.3746059/150‑1 (MQ=60)
                             CGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTG                                                                                                                        <  SRR19973079.2473037/150‑1 (MQ=60)
                             CGCCAACGCGCAGCCCCAGCAGTGCAGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTG                                                                                                                        <  SRR19973079.3374731/150‑1 (MQ=60)
                               CCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTC                                                                                                                      <  SRR19973079.4334321/150‑1 (MQ=60)
                                 AACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCAC                                                                                                                    >  SRR19973079.3060248/1‑150 (MQ=60)
                                   CGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCA                                                                                                                  <  SRR19973079.3656271/150‑1 (MQ=60)
                                   CGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCA                                                                                                                  <  SRR19973079.3656544/150‑1 (MQ=60)
                                     CGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACG                                                                                                                <  SRR19973079.2282668/150‑1 (MQ=60)
                                     CGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACG                                                                                                                <  SRR19973079.1552220/150‑1 (MQ=60)
                                     CGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACG                                                                                                                <  SRR19973079.2329066/150‑1 (MQ=60)
                                     CGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACG                                                                                                                <  SRR19973079.2329068/150‑1 (MQ=60)
                                     CGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACG                                                                                                                <  SRR19973079.1552328/150‑1 (MQ=60)
                                     CGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACG                                                                                                                <  SRR19973079.1552336/150‑1 (MQ=60)
                                       CAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTC                                                                                                              <  SRR19973079.1123123/150‑1 (MQ=60)
                                          CCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTG                                                                                                           <  SRR19973079.3626522/150‑1 (MQ=60)
                                                   TGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTC                                                                                                  <  SRR19973079.166640/150‑1 (MQ=60)
                                                    GCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCT                                                                                                 <  SRR19973079.348057/150‑1 (MQ=60)
                                                    GCGGCACCGACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCC                                                                                                 <  SRR19973079.2791502/150‑1 (MQ=60)
                                                     TGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTT                                                                                                >  SRR19973079.2669859/1‑150 (MQ=60)
                                                      GGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTC                                                                                               >  SRR19973079.2390832/1‑150 (MQ=60)
                                                      GGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTC                                                                                               <  SRR19973079.755395/150‑1 (MQ=60)
                                                      GGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTC                                                                                               <  SRR19973079.2855530/150‑1 (MQ=60)
                                                        CACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTG                                                                                             >  SRR19973079.2028301/1‑150 (MQ=60)
                                                        CACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTG                                                                                             >  SRR19973079.2950209/1‑150 (MQ=60)
                                                          CCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGC                                                                                           <  SRR19973079.2633760/150‑1 (MQ=60)
                                                           CTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCG                                                                                          <  SRR19973079.3106625/150‑1 (MQ=60)
                                                            TACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGA                                                                                         >  SRR19973079.660931/1‑150 (MQ=60)
                                                            TACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGA                                                                                         <  SRR19973079.896421/150‑1 (MQ=60)
                                                            TACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGA                                                                                         <  SRR19973079.896891/150‑1 (MQ=60)
                                                             ACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAA                                                                                        <  SRR19973079.1824612/150‑1 (MQ=60)
                                                             ACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAA                                                                                        <  SRR19973079.1827386/150‑1 (MQ=60)
                                                                GGAGCCATAACGGAAAGCTGAGTAGTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACAC                                                                                     <  SRR19973079.3796957/150‑1 (MQ=60)
                                                                  AGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAT                                                                                   <  SRR19973079.2304122/150‑1 (MQ=60)
                                                                  AGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAT                                                                                   <  SRR19973079.2304115/150‑1 (MQ=60)
                                                                   GCCATAACGGAAAGCTGAGTATTGCTATAGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATT                                                                                  <  SRR19973079.1104028/150‑1 (MQ=60)
                                                                    CCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTT                                                                                 <  SRR19973079.4262706/150‑1 (MQ=60)
                                                                    CCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTT                                                                                 <  SRR19973079.4262646/150‑1 (MQ=60)
                                                                     CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                <  SRR19973079.4328463/150‑1 (MQ=60)
                                                                     CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                <  SRR19973079.1064080/150‑1 (MQ=60)
                                                                     CATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTG                                                                                <  SRR19973079.1063959/150‑1 (MQ=60)
                                                                         ACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCG                                                                            <  SRR19973079.978258/150‑1 (MQ=60)
                                                                          CGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGC                                                                           >  SRR19973079.2691725/1‑150 (MQ=60)
                                                                           GGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCG                                                                          >  SRR19973079.927758/1‑150 (MQ=60)
                                                                             AAAGCTGAGTATTGCTATCGGTCATTTATGCCGGATACACCAGCGTGCGCACACGCACTACGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAAATCTTCTGGCGAACACAATTGGGCccgaa                                                                        >  SRR19973079.517968/1‑145 (MQ=60)
                                                                              AAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATC                                                                       >  SRR19973079.1660407/1‑150 (MQ=60)
                                                                                 CTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAA                                                                    >  SRR19973079.536293/1‑150 (MQ=60)
                                                                                      TATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTG                                                               >  SRR19973079.3192776/1‑150 (MQ=60)
                                                                                      TATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTG                                                               >  SRR19973079.3192762/1‑150 (MQ=60)
                                                                                             ATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGACCACATTTGGGCGCGATCCAACTCTGCGTTTAA                                                        >  SRR19973079.2316265/1‑150 (MQ=60)
                                                                                              TCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAC                                                       <  SRR19973079.1929408/150‑1 (MQ=60)
                                                                                                    ATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCG                                                 <  SRR19973079.727575/150‑1 (MQ=60)
                                                                                                        TTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGA                                             <  SRR19973079.319934/150‑1 (MQ=60)
                                                                                                        TTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGA                                             >  SRR19973079.3743270/1‑150 (MQ=60)
                                                                                                        TTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGA                                             >  SRR19973079.3704601/1‑150 (MQ=60)
                                                                                                        TTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGA                                             <  SRR19973079.319921/150‑1 (MQ=60)
                                                                                                        TTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGGTTAACGCGTCGGCGA                                             >  SRR19973079.3006571/1‑150 (MQ=60)
                                                                                                        TTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGATTAACGCGTCGGCGA                                             >  SRR19973079.4008317/1‑150 (MQ=60)
                                                                                                         TGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAT                                            >  SRR19973079.447032/1‑150 (MQ=60)
                                                                                                             GGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTGTAACGCGTCGGCGATTGGC                                        >  SRR19973079.3355295/1‑150 (MQ=60)
                                                                                                               ATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAA                                      >  SRR19973079.2378667/1‑150 (MQ=60)
                                                                                                                TACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAA                                     >  SRR19973079.768974/1‑150 (MQ=60)
                                                                                                                TACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAA                                     <  SRR19973079.283419/150‑1 (MQ=60)
                                                                                                                TACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAA                                     >  SRR19973079.769004/1‑150 (MQ=60)
                                                                                                                TACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAA                                     >  SRR19973079.4150402/1‑150 (MQ=60)
                                                                                                                TACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGGCGGCGATTGGCAAA                                     >  SRR19973079.770743/1‑150 (MQ=60)
                                                                                                                  CACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACC                                   <  SRR19973079.1989368/150‑1 (MQ=60)
                                                                                                                   ACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCA                                  >  SRR19973079.3047878/1‑150 (MQ=60)
                                                                                                                   ACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCA                                  >  SRR19973079.317337/1‑150 (MQ=60)
                                                                                                                    CCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAG                                 <  SRR19973079.2188871/150‑1 (MQ=60)
                                                                                                                    CCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAG                                 <  SRR19973079.3917554/150‑1 (MQ=60)
                                                                                                                     CAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGC                                >  SRR19973079.67496/1‑150 (MQ=60)
                                                                                                                      AGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTTGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCA                               >  SRR19973079.909370/1‑150 (MQ=60)
                                                                                                                      AGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTTGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCA                               >  SRR19973079.909297/1‑150 (MQ=60)
                                                                                                                        CGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATACAACTCTGCGTTTAACGCGTCGGCGATTGTCAAACCAGCATA                             >  SRR19973079.1312685/1‑150 (MQ=60)
                                                                                                                         GTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAG                            <  SRR19973079.4271629/150‑1 (MQ=60)
                                                                                                                         GTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAG                            <  SRR19973079.4271571/150‑1 (MQ=60)
                                                                                                                             GCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGG                        <  SRR19973079.3677955/150‑1 (MQ=60)
                                                                                                                              CACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGG                       <  SRR19973079.1927332/150‑1 (MQ=60)
                                                                                                                               ACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGT                      <  SRR19973079.940557/150‑1 (MQ=60)
                                                                                                                                CACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTT                     <  SRR19973079.1297150/150‑1 (MQ=60)
                                                                                                                                CACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTT                     >  SRR19973079.2513289/1‑150 (MQ=60)
                                                                                                                                CACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTT                     <  SRR19973079.4202486/150‑1 (MQ=60)
                                                                                                                                CACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTT                     <  SRR19973079.4221571/150‑1 (MQ=60)
                                                                                                                                CACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTT                     <  SRR19973079.4221581/150‑1 (MQ=60)
                                                                                                                                  CGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCACACCAGCATAGGCGGGTTGC                   >  SRR19973079.1608146/1‑150 (MQ=60)
                                                                                                                                   GCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCT                  >  SRR19973079.2690035/1‑150 (MQ=60)
                                                                                                                                     ACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCC                <  SRR19973079.2908553/150‑1 (MQ=60)
                                                                                                                                           CCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGA          >  SRR19973079.2932169/1‑150 (MQ=60)
                                                                                                                                            CATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAA         >  SRR19973079.816211/1‑150 (MQ=60)
                                                                                                                                               CGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATAT      >  SRR19973079.693279/1‑150 (MQ=60)
                                                                                                                                               CGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATAT      >  SRR19973079.693349/1‑150 (MQ=60)
                                                                                                                                                   AAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGAT  <  SRR19973079.244073/150‑1 (MQ=60)
                                                                                                                                                   AAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGAT  >  SRR19973079.1307060/1‑150 (MQ=60)
                                                                                                                                                   AAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGAT  >  SRR19973079.501077/1‑150 (MQ=60)
                                                                                                                                                   AAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGAT  >  SRR19973079.3152014/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATATCGAT  >  CP009273/639082‑639378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑