Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F394 I0 R1	1342	215.0	7134094	96.2%	6862998	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,712,562	C→A	63.2%	D31Y (GAT→TAT)	mliC ←	inhibitor of c‑type lysozyme, membrane‑bound; putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,712,562	0	C	A	63.2%	120.4 / 297.1	231	D31Y (GAT→TAT)	mliC	inhibitor of c‑type lysozyme, membrane‑bound; putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (45/40); new base A (78/68); total (123/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 233 total aligned reads displayed.

ATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGT  >  CP009273/1712417‑1712707

agtCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCgg                                                                                                                                                 <  2:1404015/146‑1 (MQ=255)

aTTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAgg                                                                                                                                                 >  2:2413828/1‑148 (MQ=255)

 ttCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGt                                                                                                                                                <  1:3359206/148‑1 (MQ=255)

 ttCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGt                                                                                                                                                >  2:1377302/1‑148 (MQ=255)

    cGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTg                                                                                                                                             >  1:3461349/1‑148 (MQ=255)

          tgtAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATgcg                                                                                                                                       >  1:2667076/1‑148 (MQ=255)

               cgcgcgCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCaa                                                                                                                                  >  1:2568336/1‑148 (MQ=255)

                       agagGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGa                                                                                                                          >  1:102055/1‑148 (MQ=255)

                       agagGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGa                                                                                                                          <  1:482794/148‑1 (MQ=255)

                       agagGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGa                                                                                                                          <  2:2964808/148‑1 (MQ=255)

                        gagGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGAt                                                                                                                         <  2:1963091/148‑1 (MQ=255)

                            cTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTaaa                                                                                                                     >  1:1606011/1‑148 (MQ=255)

                             tGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAg                                                                                                                    <  2:769918/148‑1 (MQ=255)

                              gAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAgg                                                                                                                   >  1:2602454/1‑148 (MQ=255)

                                aaTGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGgcg                                                                                                                 <  1:1338683/148‑1 (MQ=255)

                                   gcacTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTa                                                                                                              >  2:3159374/4‑148 (MQ=255)

                                   gCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTa                                                                                                              <  2:2171847/148‑1 (MQ=255)

                                              gaTGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGgagag                                                                                                   >  2:1193080/1‑148 (MQ=255)

                                                 gCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAg                                                                                                >  2:2434521/1‑148 (MQ=255)

                                                 gCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAg                                                                                                <  1:647115/148‑1 (MQ=255)

                                                   agtagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGa                                                                                              <  1:1001447/148‑1 (MQ=255)

                                                     tagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAATGAcc                                                                                            >  2:3240383/1‑148 (MQ=255)

                                                     tagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGAcc                                                                                            >  1:524283/1‑148 (MQ=255)

                                                     tagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGAcc                                                                                            >  1:3115476/1‑148 (MQ=255)

                                                      agtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCg                                                                                           <  2:102055/148‑1 (MQ=255)

                                                      agtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCg                                                                                           >  1:2283037/1‑148 (MQ=255)

                                                       gtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCgg                                                                                          >  2:3520180/1‑148 (MQ=255)

                                                       gtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCgg                                                                                          <  1:2582336/148‑1 (MQ=255)

                                                         tGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCa                                                                                        >  2:796017/1‑148 (MQ=255)

                                                           aTTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCaaa                                                                                      <  1:1850510/148‑1 (MQ=255)

                                                           aTTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCaaa                                                                                      <  2:3461349/148‑1 (MQ=255)

                                                           aTTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCaaa                                                                                      >  1:1292044/1‑148 (MQ=255)

                                                             tATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAAt                                                                                    >  1:2022624/1‑148 (MQ=255)

                                                                cGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCACAATGAt                                                                                 >  1:1201537/1‑148 (MQ=255)

                                                                cGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGAt                                                                                 <  1:1788505/148‑1 (MQ=255)

                                                                 gTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATa                                                                                <  1:560988/148‑1 (MQ=255)

                                                                 gTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATa                                                                                <  1:276486/148‑1 (MQ=255)

                                                                  tAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATaa                                                                               >  2:2571812/1‑148 (MQ=255)

                                                                  tAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATaa                                                                               <  2:877454/148‑1 (MQ=255)

                                                                  tAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATaa                                                                               >  1:1056584/1‑148 (MQ=255)

                                                                  tAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATaa                                                                               >  1:139882/1‑148 (MQ=255)

                                                                   aaaCAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAAt                                                                              >  1:3367218/1‑148 (MQ=255)

                                                                    aaCAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAAtt                                                                             <  2:3379432/148‑1 (MQ=255)

                                                                    aaCAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAAtt                                                                             >  1:3053110/1‑148 (MQ=255)

                                                                      cAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTaa                                                                           >  1:555200/1‑148 (MQ=255)

                                                                      cAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTaa                                                                           <  2:2495474/148‑1 (MQ=255)

                                                                      cAAAACTGACCTCCTGGCGCGGATTATTCAGTGTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTaa                                                                           >  2:2628720/1‑148 (MQ=255)

                                                                       aaaaCTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCCACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGATTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATGAAc                                                                          >  1:3540121/1‑148 (MQ=255)

                                                                         aaCTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAg                                                                        >  1:1079622/1‑148 (MQ=255)

                                                                         aaCTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAg                                                                        >  1:2140581/1‑148 (MQ=255)

                                                                          aCTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGt                                                                       >  2:3289908/1‑148 (MQ=255)

                                                                          aCTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGt                                                                       <  1:1539203/148‑1 (MQ=255)

                                                                           cTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGtt                                                                      >  1:1245142/1‑148 (MQ=255)

                                                                             gACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGtttt                                                                    >  2:1268319/1‑148 (MQ=255)

                                                                              aCCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGttttt                                                                   >  1:1564721/1‑148 (MQ=255)

                                                                              aCCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGttttt                                                                   <  2:1898375/148‑1 (MQ=255)

                                                                                ctcctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTc                                                                 <  2:1228866/148‑1 (MQ=255)

                                                                                  cctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCAt                                                               >  2:595493/1‑148 (MQ=255)

                                                                                  cctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCAt                                                               <  1:2023287/148‑1 (MQ=255)

                                                                                  cctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCAt                                                               >  2:2262192/1‑148 (MQ=255)

                                                                                   ctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATa                                                              <  1:386378/148‑1 (MQ=255)

                                                                                   ctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATa                                                              <  2:404821/148‑1 (MQ=255)

                                                                                    tGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAg                                                             >  2:1815171/1‑148 (MQ=255)

                                                                                      gcgcGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTc                                                           >  2:728189/1‑148 (MQ=255)

                                                                                      gcgcGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTc                                                           >  2:1309221/1‑148 (MQ=255)

                                                                                           gATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATc                                                      <  1:85503/148‑1 (MQ=255)

                                                                                            attattCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATcc                                                     <  2:1725442/148‑1 (MQ=255)

                                                                                               attCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGa                                                  <  2:3177276/148‑1 (MQ=255)

                                                                                               attCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGa                                                  <  2:1056637/148‑1 (MQ=255)

                                                                                               attCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGa                                                  <  2:1568234/148‑1 (MQ=255)

                                                                                                      ttGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACttt                                           >  2:1071688/1‑148 (MQ=255)

                                                                                                        gACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTcc                                         <  2:2039707/148‑1 (MQ=255)

                                                                                                        gACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTcc                                         <  1:216282/148‑1 (MQ=255)

                                                                                                        gACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTcc                                         >  2:629311/1‑148 (MQ=255)

                                                                                                          ccGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTg                                       <  1:2622107/148‑1 (MQ=255)

                                                                                                             tCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTc                                    <  2:524283/148‑1 (MQ=255)

                                                                                                             tCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTc                                    <  2:3115476/148‑1 (MQ=255)

                                                                                                                aCGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTgg                                 >  1:561788/1‑148 (MQ=255)

                                                                                                                   gTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAgg                              >  2:3133888/1‑148 (MQ=255)

                                                                                                                    tttttCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAggg                             >  1:2351096/1‑148 (MQ=255)

                                                                                                                     ttttCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGc                            >  2:2608156/1‑148 (MQ=255)

                                                                                                                     ttttCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGc                            >  1:2770756/1‑148 (MQ=255)

                                                                                                                     ttttCATCACACTGGTATCCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGc                            >  1:552416/1‑148 (MQ=255)

                                                                                                                      tttCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCa                           <  2:2946738/148‑1 (MQ=255)

                                                                                                                       ttCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCaa                          <  2:1739274/148‑1 (MQ=255)

                                                                                                                        tcatcaCACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAAt                         <  2:1623810/148‑1 (MQ=255)

                                                                                                                         catcaCACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATa                        >  1:1059515/1‑148 (MQ=255)

                                                                                                                          atcaCACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAAACAGCTGATTAAATGCGCTACAGCAGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTATCAGAGTAATATCCCGAAGAATTACCAGGTATGGAGGGCAATAc                       >  1:716471/1‑148 (MQ=255)

                                                                                                                           tcaCACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACg                      >  2:188738/1‑148 (MQ=255)

                                                                                                                           tcaCACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACg                      >  2:1827843/1‑148 (MQ=255)

                                                                                                                            cacaCTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGc                     >  1:3211322/1‑148 (MQ=255)

                                                                                                                                 tGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccctcc                <  1:2425354/148‑1 (MQ=255)

                                                                                                                                 tGGTAGTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccctcc                <  2:1759660/148‑1 (MQ=255)

                                                                                                                                  ggTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccctccc               <  2:2786395/148‑1 (MQ=255)

                                                                                                                                     aTTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTaa            <  1:2128610/148‑1 (MQ=255)

                                                                                                                                       tCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACg          <  1:3159374/148‑1 (MQ=255)

                                                                                                                                           gCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTcc      >  2:1489364/1‑148 (MQ=255)

                                                                                                                                            cGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCa     <  2:1844901/148‑1 (MQ=255)

                                                                                                                                              tataGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAg   >  2:657752/1‑148 (MQ=255)

                                                                                                                                               aTCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAgt  <  1:1676326/148‑1 (MQ=255)

ATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGT  >  CP009273/1712417‑1712707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGT  >  CP009273/1712415‑1712709

AGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGG                                                                                                                                                   >  SRR19973066.3198422/1‑150 (MQ=60)

  GTTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTT                                                                                                                                                 <  SRR19973066.2588338/150‑1 (MQ=60)

   TTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTT                                                                                                                                                <  SRR19973066.3381606/150‑1 (MQ=60)

    TCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTG                                                                                                                                               >  SRR19973066.3484490/1‑150 (MQ=60)

    TCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTG                                                                                                                                               <  SRR19973066.492350/150‑1 (MQ=60)

          AGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCG                                                                                                                                         >  SRR19973066.424857/1‑150 (MQ=60)

          AGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCG                                                                                                                                         >  SRR19973066.2684920/1‑150 (MQ=60)

             GTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTA                                                                                                                                      <  SRR19973066.1078280/150‑1 (MQ=60)

               AACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAA                                                                                                                                    >  SRR19973066.1911918/1‑150 (MQ=60)

               AACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAA                                                                                                                                    >  SRR19973066.2585763/1‑150 (MQ=60)

                  GCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCA                                                                                                                                 <  SRR19973066.2789091/150‑1 (MQ=60)

                       CCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGA                                                                                                                            >  SRR19973066.103138/1‑150 (MQ=60)

                         AGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATT                                                                                                                          <  SRR19973066.486418/150‑1 (MQ=60)

                            GGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTACA                                                                                                                       >  SRR19973066.1075214/1‑150 (MQ=60)

                            GGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAA                                                                                                                       >  SRR19973066.1617663/1‑150 (MQ=60)

                            GGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAA                                                                                                                       >  SRR19973066.1617856/1‑150 (MQ=60)

                              CTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGG                                                                                                                     >  SRR19973066.2620058/1‑150 (MQ=60)

                                  AATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCT                                                                                                                 <  SRR19973066.1348398/150‑1 (MQ=60)

                                   ATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTA                                                                                                                <  SRR19973066.380484/150‑1 (MQ=60)

                                                  TGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGG                                                                                                 <  SRR19973066.2875532/150‑1 (MQ=60)

                                                   GCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGA                                                                                                <  SRR19973066.651807/150‑1 (MQ=60)

                                                    CAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGAC                                                                                               >  SRR19973066.3385574/1‑150 (MQ=60)

                                                    CAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGAC                                                                                               <  SRR19973066.2430179/150‑1 (MQ=60)

                                                    CAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGAC                                                                                               <  SRR19973066.1483399/150‑1 (MQ=60)

                                                     AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                              >  SRR19973066.902038/1‑150 (MQ=60)

                                                     AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                              <  SRR19973066.1008829/150‑1 (MQ=60)

                                                     AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                              >  SRR19973066.3136282/1‑150 (MQ=60)

                                                     AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                              >  SRR19973066.528128/1‑150 (MQ=60)

                                                     AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                              >  SRR19973066.529150/1‑150 (MQ=60)

                                                     AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                              <  SRR19973066.1009135/150‑1 (MQ=60)

                                                      GTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCG                                                                                             >  SRR19973066.2298716/1‑150 (MQ=60)

                                                      GTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCG                                                                                             >  SRR19973066.3145664/1‑150 (MQ=60)

                                                      GTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGAATAGGTTTGCAAGCTCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCG                                                                                             >  SRR19973066.1567778/1‑150 (MQ=60)

                                                         GTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCA                                                                                          <  SRR19973066.2599840/150‑1 (MQ=60)

                                                          TTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAA                                                                                         >  SRR19973066.650010/1‑150 (MQ=60)

                                                           TGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAA                                                                                        >  SRR19973066.1301397/1‑150 (MQ=60)

                                                           TGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAA                                                                                        >  SRR19973066.1301324/1‑150 (MQ=60)

                                                             ATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAAT                                                                                      <  SRR19973066.1863754/150‑1 (MQ=60)

                                                             ATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAAT                                                                                      >  SRR19973066.780548/1‑150 (MQ=60)

                                                             ATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAAT                                                                                      >  SRR19973066.2036770/1‑150 (MQ=60)

                                                             ATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAAT                                                                                      <  SRR19973066.285313/150‑1 (MQ=60)

                                                               TATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGA                                                                                    >  SRR19973066.1281601/1‑150 (MQ=60)

                                                                ATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCACAATGAT                                                                                   >  SRR19973066.1209904/1‑150 (MQ=60)

                                                                  CGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAA                                                                                 <  SRR19973066.1801252/150‑1 (MQ=60)

                                                                  CGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAA                                                                                 <  SRR19973066.694309/150‑1 (MQ=60)

                                                                  CGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAA                                                                                 >  SRR19973066.1064402/1‑150 (MQ=60)

                                                                  CGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAA                                                                                 >  SRR19973066.1064349/1‑150 (MQ=60)

                                                                  CGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAA                                                                                 >  SRR19973066.141231/1‑150 (MQ=60)

                                                                  CGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAA                                                                                 >  SRR19973066.1579533/1‑150 (MQ=60)

                                                                   GTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAAT                                                                                <  SRR19973066.564992/150‑1 (MQ=60)

                                                                   GTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAAT                                                                                >  SRR19973066.3389669/1‑150 (MQ=60)

                                                                   GTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAAT                                                                                <  SRR19973066.278510/150‑1 (MQ=60)

                                                                    TAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGACGGACCGGCAAAATGATAATT                                                                               >  SRR19973066.3073531/1‑150 (MQ=60)

                                                                      AACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAA                                                                             >  SRR19973066.559181/1‑150 (MQ=60)

                                                                      AACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAA                                                                             >  SRR19973066.559163/1‑150 (MQ=60)

                                                                      AACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAA                                                                             >  SRR19973066.559160/1‑150 (MQ=60)

                                                                       ACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCCACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGATTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATGAAC                                                                            >  SRR19973066.3563643/1‑150 (MQ=60)

                                                                       ACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAAC                                                                            <  SRR19973066.2596525/150‑1 (MQ=60)

                                                                         AAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAG                                                                          >  SRR19973066.1087511/1‑150 (MQ=60)

                                                                         AAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAG                                                                          >  SRR19973066.3428972/1‑150 (MQ=60)

                                                                         AAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAG                                                                          >  SRR19973066.2155436/1‑150 (MQ=60)

                                                                          AAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGT                                                                         <  SRR19973066.2604392/150‑1 (MQ=60)

                                                                           AACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGNTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTT                                                                        <  SRR19973066.1820895/150‑1 (MQ=60)

                                                                           AACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTT                                                                        >  SRR19973066.1254064/1‑150 (MQ=60)

                                                                           AACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTT                                                                        >  SRR19973066.1254052/1‑150 (MQ=60)

                                                                            ACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTT                                                                       <  SRR19973066.2292738/150‑1 (MQ=60)

                                                                            ACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTT                                                                       <  SRR19973066.1550198/150‑1 (MQ=60)

                                                                             CTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTT                                                                      <  SRR19973066.3087257/150‑1 (MQ=60)

                                                                             CTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTT                                                                      >  SRR19973066.1197152/1‑150 (MQ=60)

                                                                              TGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTT                                                                     >  SRR19973066.1575992/1‑150 (MQ=60)

                                                                              TGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTT                                                                     >  SRR19973066.2148187/1‑150 (MQ=60)

                                                                              TGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTT                                                                     >  SRR19973066.2148547/1‑150 (MQ=60)

                                                                              TGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTT                                                                     <  SRR19973066.2528607/150‑1 (MQ=60)

                                                                              TGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTT                                                                     >  SRR19973066.1751823/1‑150 (MQ=60)

                                                                                    CCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAG                                                               <  SRR19973066.2037025/150‑1 (MQ=60)

                                                                                    CCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAG                                                               <  SRR19973066.2037435/150‑1 (MQ=60)

                                                                                     CTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGT                                                              <  SRR19973066.389438/150‑1 (MQ=60)

                                                                                     CTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGT                                                              <  SRR19973066.2640517/150‑1 (MQ=60)

                                                                                         CGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTAGTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATA                                                          <  SRR19973066.3417904/150‑1 (MQ=60)

                                                                                           CGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATC                                                        >  SRR19973066.1053255/1‑150 (MQ=60)

                                                                                           CGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATC                                                        >  SRR19973066.2740815/1‑150 (MQ=60)

                                                                                            GGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCC                                                       <  SRR19973066.2013237/150‑1 (MQ=60)

                                                                                             GATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCC                                                      <  SRR19973066.86448/150‑1 (MQ=60)

                                                                                                     AGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTGTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTT                                              <  SRR19973066.3539469/150‑1 (MQ=60)

                                                                                                          GACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTG                                         <  SRR19973066.218059/150‑1 (MQ=60)

                                                                                                          GACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTG                                         <  SRR19973066.216973/150‑1 (MQ=60)

                                                                                                          GACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTG                                         <  SRR19973066.1450340/150‑1 (MQ=60)

                                                                                                            CCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGT                                       <  SRR19973066.2639822/150‑1 (MQ=60)

                                                                                                            CCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGT                                       <  SRR19973066.2639796/150‑1 (MQ=60)

                                                                                                                CAACGGTTTTTCATCACACTGGTATTCCAGCTTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGG                                   >  SRR19973066.464921/1‑150 (MQ=60)

                                                                                                                CAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGG                                   >  SRR19973066.564824/1‑150 (MQ=60)

                                                                                                                CAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGG                                   >  SRR19973066.565737/1‑150 (MQ=60)

                                                                                                                CAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGG                                   >  SRR19973066.565796/1‑150 (MQ=60)

                                                                                                                    GGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGG                               >  SRR19973066.2366985/1‑150 (MQ=60)

                                                                                                                    GGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGG                               >  SRR19973066.2367067/1‑150 (MQ=60)

                                                                                                                     GTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGC                              >  SRR19973066.2789413/1‑150 (MQ=60)

                                                                                                                     GTTTTTCATCACACTGGTATCCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGC                              >  SRR19973066.556383/1‑150 (MQ=60)

                                                                                                                         TTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATA                          >  SRR19973066.1067295/1‑150 (MQ=60)

                                                                                                                         TTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATA                          >  SRR19973066.1408314/1‑150 (MQ=60)

                                                                                                                          TCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAAACAGCTGATTAAATGCGCTACAGCAGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTATCAGAGTAATATCCCGAAGAATTACCAGGTATGGAGGGCAATAC                         >  SRR19973066.721774/1‑150 (MQ=60)

                                                                                                                            ATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGC                       >  SRR19973066.3232255/1‑150 (MQ=60)

                                                                                                                            ATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGC                       >  SRR19973066.3263781/1‑150 (MQ=60)

                                                                                                                            ATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGC                       >  SRR19973066.3232603/1‑150 (MQ=60)

                                                                                                                            ATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGC                       >  SRR19973066.2325719/1‑150 (MQ=60)

                                                                                                                              CACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCC                     >  SRR19973066.1321167/1‑150 (MQ=60)

                                                                                                                                   TGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCT                <  SRR19973066.637842/150‑1 (MQ=60)

                                                                                                                                   TGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCT                <  SRR19973066.2441826/150‑1 (MQ=60)

                                                                                                                                      TATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAAC             >  SRR19973066.262535/1‑150 (MQ=60)

                                                                                                                                       ATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACG            <  SRR19973066.2143357/150‑1 (MQ=60)

                                                                                                                                         TCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTT          <  SRR19973066.3180427/150‑1 (MQ=60)

                                                                                                                                            AGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCA       >  SRR19973066.1634078/1‑150 (MQ=60)

                                                                                                                                             GCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAA      <  SRR19973066.2077573/150‑1 (MQ=60)

                                                                                                                                                 ATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGT  <  SRR19973066.1688536/150‑1 (MQ=60)

AGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGT  >  CP009273/1712415‑1712709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`