Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,712,562 C→A 100% D31Y (GAT→TAT)  mliC ← inhibitor of c‑type lysozyme, membrane‑bound; putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,712,5620CA99.4% 539.4 / ‑4.2 165D31Y (GAT→TAT) mliCinhibitor of c‑type lysozyme, membrane‑bound; putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (68/96);  total (69/96)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

TTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAG  >  CP009273/1712418‑1712706
                                                                                                                                                |                                                                                                                                                
ttCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGt                                                                                                                                               <  1:1849924/148‑1 (MQ=255)
  ccGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGttt                                                                                                                                             <  2:1519279/148‑1 (MQ=255)
   cGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTg                                                                                                                                            <  1:629524/148‑1 (MQ=255)
      cAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCAt                                                                                                                                         >  2:756469/1‑148 (MQ=255)
      cAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCAt                                                                                                                                         >  2:865783/1‑148 (MQ=255)
      cAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCAt                                                                                                                                         <  1:2402700/148‑1 (MQ=255)
       aGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATg                                                                                                                                        <  1:244087/148‑1 (MQ=255)
         tgtAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATgcg                                                                                                                                      <  2:271030/148‑1 (MQ=255)
         tgtAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATgcg                                                                                                                                      >  1:2714995/1‑148 (MQ=255)
           tAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCt                                                                                                                                    >  1:2465910/1‑148 (MQ=255)
              cgcgcgCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCaa                                                                                                                                 <  2:2585146/148‑1 (MQ=255)
              cgcgcgCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGGATTCCAGCGTATAGGTTTGCATGCGCTCaa                                                                                                                                 >  1:1797673/1‑148 (MQ=255)
                  cgcCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAg                                                                                                                             >  2:3422473/1‑148 (MQ=255)
                      agagGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGa                                                                                                                         <  2:251608/148‑1 (MQ=255)
                        agGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGAtt                                                                                                                       >  1:2879797/1‑148 (MQ=255)
                         ggCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTa                                                                                                                      <  1:2836040/148‑1 (MQ=255)
                         ggCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTa                                                                                                                      <  1:3215331/148‑1 (MQ=255)
                          gCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTaa                                                                                                                     >  1:3016898/1‑148 (MQ=255)
                            tGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAg                                                                                                                   <  2:2368264/148‑1 (MQ=255)
                               aaTGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGgcg                                                                                                                >  1:2022413/1‑148 (MQ=255)
                                aTGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGgcgc                                                                                                               >  2:3314972/1‑148 (MQ=255)
                                aTGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGgcgc                                                                                                               <  2:858713/148‑1 (MQ=255)
                                  gCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTa                                                                                                             <  1:2343565/148‑1 (MQ=255)
                                  gCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTa                                                                                                             <  1:2591669/148‑1 (MQ=255)
                                  gCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTa                                                                                                             <  1:2595906/148‑1 (MQ=255)
                                  gCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTa                                                                                                             <  1:887862/148‑1 (MQ=255)
                                     cTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAg                                                                                                          <  2:2773373/148‑1 (MQ=255)
                                      tGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGc                                                                                                         >  1:1474973/1‑148 (MQ=255)
                                          tGAGATGCAGTAGTTGATTCTCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGga                                                                                                     <  1:975891/148‑1 (MQ=255)
                                           gagaTGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGgag                                                                                                    <  2:3019122/148‑1 (MQ=255)
                                            agaTGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGgaga                                                                                                   >  2:3332066/1‑148 (MQ=255)
                                              aTGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGgagaga                                                                                                 <  2:402452/148‑1 (MQ=255)
                                              aTGAAGTAGTTGAGTTTAGTAAACAAAACTGACCTACTGTCGAGTATTATTCAGTTTGACCGTCATCGGTTTTTCATCACACTTGTATTCCAGCGTTTAGGTTTGCATGCGCTCAACCATCTGATTAAAGGCGCTACAGCCGgagaga                                                                                                 <  2:2174294/148‑1 (MQ=255)
                                                gCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAg                                                                                               >  1:3231271/1‑148 (MQ=255)
                                                  agtagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAATGCGCTACAGCCGGAGAGAAGGa                                                                                             >  1:3355513/1‑148 (MQ=255)
                                                  agtagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGa                                                                                             <  1:3282925/148‑1 (MQ=255)
                                                  agtagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGa                                                                                             <  1:3282974/148‑1 (MQ=255)
                                                   gtagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGAc                                                                                            >  2:1872804/1‑148 (MQ=255)
                                                    tagtTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGAcc                                                                                           >  1:1979172/1‑148 (MQ=255)
                                                         gATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCaa                                                                                      >  1:278171/1‑148 (MQ=255)
                                                         gATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCaa                                                                                      >  1:278149/1‑148 (MQ=255)
                                                           ttATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGTCGGAGAGAAGGACCGGCaaaa                                                                                    <  1:3419404/148‑1 (MQ=255)
                                                           ttATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCaaaa                                                                                    >  2:642432/1‑148 (MQ=255)
                                                            tATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAAt                                                                                   >  2:906866/1‑148 (MQ=255)
                                                              tCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGa                                                                                 >  1:1230505/1‑148 (MQ=255)
                                                              tCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGa                                                                                 >  1:1231897/1‑148 (MQ=255)
                                                              tCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGa                                                                                 <  1:932972/148‑1 (MQ=255)
                                                              tCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGa                                                                                 >  1:2377797/1‑148 (MQ=255)
                                                                gTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATa                                                                               <  1:395550/148‑1 (MQ=255)
                                                                 tAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATaa                                                                              >  1:2181055/1‑148 (MQ=255)
                                                                 tAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATaa                                                                              <  1:2974449/148‑1 (MQ=255)
                                                                 tAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATaa                                                                              <  1:2550341/148‑1 (MQ=255)
                                                                    aCAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTa                                                                           >  1:842014/1‑148 (MQ=255)
                                                                    aCAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTa                                                                           <  2:2465375/148‑1 (MQ=255)
                                                                    aCAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTa                                                                           <  2:1738089/148‑1 (MQ=255)
                                                                          cTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGtt                                                                     <  1:1393013/148‑1 (MQ=255)
                                                                            gACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGtttt                                                                   >  2:2414287/1‑148 (MQ=255)
                                                                            gACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGtttt                                                                   <  2:2864724/148‑1 (MQ=255)
                                                                               ctcctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTTCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTc                                                                <  2:3234111/148‑1 (MQ=255)
                                                                               ctcctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTc                                                                <  1:1164205/148‑1 (MQ=255)
                                                                               ctcctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTc                                                                <  1:2134853/148‑1 (MQ=255)
                                                                               ctcctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTc                                                                >  2:2940666/1‑148 (MQ=255)
                                                                               ctcctGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTc                                                                >  2:2316902/1‑148 (MQ=255)
                                                                                   tGGCTCTGATTATTCATTTTGACCGTCAACGTTTTTTCATCACACTTGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAg                                                            <  1:2230711/148‑1 (MQ=255)
                                                                                   tGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAg                                                            >  2:2014717/1‑148 (MQ=255)
                                                                                    ggCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGt                                                           <  1:838279/148‑1 (MQ=255)
                                                                                     gcgcGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTc                                                          >  1:2505992/1‑148 (MQ=255)
                                                                                        cgGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCata                                                       >  2:3259284/1‑148 (MQ=255)
                                                                                         ggATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCatat                                                      <  2:2971332/148‑1 (MQ=255)
                                                                                           attattCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATcc                                                    <  1:3089153/148‑1 (MQ=255)
                                                                                            ttattCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATccc                                                   <  2:787492/148‑1 (MQ=255)
                                                                                             tattCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCg                                                  >  2:2214587/1‑148 (MQ=255)
                                                                                                 cAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGa                                              <  1:874232/148‑1 (MQ=255)
                                                                                                  aGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGAc                                             >  1:2066303/1‑148 (MQ=255)
                                                                                                      tGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTc                                         <  1:3051495/148‑1 (MQ=255)
                                                                                                         ccGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTg                                      <  2:2714995/148‑1 (MQ=255)
                                                                                                         ccGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTg                                      <  2:2715350/148‑1 (MQ=255)
                                                                                                            tCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTc                                   <  1:1099476/148‑1 (MQ=255)
                                                                                                            tCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTc                                   <  2:2504110/148‑1 (MQ=255)
                                                                                                            tCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTc                                   >  1:1386849/1‑148 (MQ=255)
                                                                                                             cAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCt                                  >  2:3408098/1‑148 (MQ=255)
                                                                                                                      ttCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCaa                         <  1:756469/148‑1 (MQ=255)
                                                                                                                       tcatcaCACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAAt                        <  2:2050987/148‑1 (MQ=255)
                                                                                                                           cacaCTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCCTAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGc                    >  2:2554599/1‑148 (MQ=255)
                                                                                                                           cacaCTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGc                    >  1:1881004/1‑148 (MQ=255)
                                                                                                                           cacaCTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGc                    >  2:2555006/1‑148 (MQ=255)
                                                                                                                             cacTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccc                  <  2:3242522/148‑1 (MQ=255)
                                                                                                                             cacTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccc                  <  2:3489902/148‑1 (MQ=255)
                                                                                                                                tGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccctcc               <  1:865783/148‑1 (MQ=255)
                                                                                                                                tGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccctcc               >  1:59643/1‑148 (MQ=255)
                                                                                                                                 ggTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccctccc              <  2:3124400/148‑1 (MQ=255)
                                                                                                                                  gTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGccctccct             >  1:487458/1‑148 (MQ=255)
                                                                                                                                   tATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTa            <  2:1230505/148‑1 (MQ=255)
                                                                                                                                   tATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTa            <  2:1231897/148‑1 (MQ=255)
                                                                                                                                       ccAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGt        <  2:619013/148‑1 (MQ=255)
                                                                                                                                       ccAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGt        <  2:2879797/148‑1 (MQ=255)
                                                                                                                                          gCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTcc     >  2:1324046/1‑148 (MQ=255)
                                                                                                                                            gTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCaa   <  1:1058436/148‑1 (MQ=255)
                                                                                                                                             tataGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAg  <  1:350059/148‑1 (MQ=255)
                                                                                                                                             tataGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAg  <  2:2013498/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAG  >  CP009273/1712418‑1712706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGTAA  >  CP009273/1712414‑1712711
                                                                                                                                                    |                                                                                                                                                     
TAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAG                                                                                                                                                      >  SRR19973065.2898555/1‑150 (MQ=60)
    TTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTT                                                                                                                                                  <  SRR19973065.1862116/150‑1 (MQ=60)
     TCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTG                                                                                                                                                 <  SRR19973065.1401904/150‑1 (MQ=60)
       CGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCA                                                                                                                                               <  SRR19973065.633622/150‑1 (MQ=60)
          CAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGC                                                                                                                                            <  SRR19973065.2417807/150‑1 (MQ=60)
           AGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCG                                                                                                                                           >  SRR19973065.2731885/1‑150 (MQ=60)
           AGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCG                                                                                                                                           <  SRR19973065.2137011/150‑1 (MQ=60)
           AGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCG                                                                                                                                           <  SRR19973065.245821/150‑1 (MQ=60)
           AGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCG                                                                                                                                           >  SRR19973065.2732243/1‑150 (MQ=60)
             TGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCT                                                                                                                                         >  SRR19973065.3342851/1‑150 (MQ=60)
             TGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCT                                                                                                                                         >  SRR19973065.2481451/1‑150 (MQ=60)
               TAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCA                                                                                                                                       >  SRR19973065.2439775/1‑150 (MQ=60)
                AACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGGATTCCAGCGTATAGGTTTGCATGCGCTCAA                                                                                                                                      >  SRR19973065.1809481/1‑150 (MQ=60)
                       GCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTG                                                                                                                               <  SRR19973065.2897036/150‑1 (MQ=60)
                          AGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATT                                                                                                                            >  SRR19973065.2897427/1‑150 (MQ=60)
                          AGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCACCTGGCGCGGATTATTCAGTTTGACCGTCAAAGGTTTTTAATCACACTGGTATTCCAGCGTATAGTTTTGCATGCGCTCAACCAGCTGATT                                                                                                                            >  SRR19973065.622993/1‑150 (MQ=60)
                            AGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAA                                                                                                                          >  SRR19973065.3035379/1‑150 (MQ=60)
                             GGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAA                                                                                                                         <  SRR19973065.2853503/150‑1 (MQ=60)
                             GGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAA                                                                                                                         <  SRR19973065.3234669/150‑1 (MQ=60)
                             GGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAA                                                                                                                         <  SRR19973065.3234682/150‑1 (MQ=60)
                                TGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGC                                                                                                                      <  SRR19973065.2812301/150‑1 (MQ=60)
                                TGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGC                                                                                                                      <  SRR19973065.560854/150‑1 (MQ=60)
                                 gctcttccgatcTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCG                                                                                                                     <  SRR19973065.2012079/138‑1 (MQ=60)
                                 GAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCG                                                                                                                     >  SRR19973065.2035421/1‑150 (MQ=60)
                                      GCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACA                                                                                                                <  SRR19973065.2612042/150‑1 (MQ=60)
                                      GCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACA                                                                                                                <  SRR19973065.2607792/150‑1 (MQ=60)
                                      GCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACA                                                                                                                <  SRR19973065.893500/150‑1 (MQ=60)
                                      GCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACA                                                                                                                <  SRR19973065.2358188/150‑1 (MQ=60)
                                        CCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGC                                                                                                              >  SRR19973065.815907/1‑150 (MQ=60)
                                        CCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGC                                                                                                              >  SRR19973065.1484409/1‑150 (MQ=60)
                                          TGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACAGTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAATGGCGCTACAGCCG                                                                                                            >  SRR19973065.3011934/1‑150 (MQ=60)
                                              TGAGATGCAGTAGTTGATTCTCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGA                                                                                                        <  SRR19973065.982375/150‑1 (MQ=60)
                                                  ATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAG                                                                                                    >  SRR19973065.3250688/1‑150 (MQ=60)
                                                    GCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAATGCGCTACAGCCGGAGAGAAGGA                                                                                                  >  SRR19973065.3376026/1‑150 (MQ=60)
                                                      AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                                <  SRR19973065.3302933/150‑1 (MQ=60)
                                                      AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                                <  SRR19973065.3302889/150‑1 (MQ=60)
                                                      AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                                <  SRR19973065.3302884/150‑1 (MQ=60)
                                                      AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                                <  SRR19973065.3180948/150‑1 (MQ=60)
                                                      AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                                >  SRR19973065.1990567/1‑150 (MQ=60)
                                                      AGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACC                                                                                                >  SRR19973065.1992044/1‑150 (MQ=60)
                                                       GTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCG                                                                                               <  SRR19973065.2178932/150‑1 (MQ=60)
                                                         AGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGC                                                                                             <  SRR19973065.886886/150‑1 (MQ=60)
                                                           TTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAA                                                                                           >  SRR19973065.280026/1‑150 (MQ=60)
                                                           TTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAA                                                                                           >  SRR19973065.280004/1‑150 (MQ=60)
                                                               TTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGTCGGAGAGAAGGACCGGCAAAATG                                                                                       <  SRR19973065.3440314/150‑1 (MQ=60)
                                                               TTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGTCGGAGAGAAGGACCGGCAAAATG                                                                                       <  SRR19973065.3440294/150‑1 (MQ=60)
                                                                TATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGA                                                                                      >  SRR19973065.1238556/1‑150 (MQ=60)
                                                                TATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGA                                                                                      >  SRR19973065.1239959/1‑150 (MQ=60)
                                                                TATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGA                                                                                      >  SRR19973065.2392659/1‑150 (MQ=60)
                                                                  TCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATA                                                                                    <  SRR19973065.939149/150‑1 (MQ=60)
                                                                  TCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATA                                                                                    <  SRR19973065.939130/150‑1 (MQ=60)
                                                                   CGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAA                                                                                   >  SRR19973065.2195045/1‑150 (MQ=60)
                                                                    GTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAAT                                                                                  >  SRR19973065.1343802/1‑150 (MQ=60)
                                                                    GTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAAT                                                                                  <  SRR19973065.398314/150‑1 (MQ=60)
                                                                     TAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATT                                                                                 <  SRR19973065.2567500/150‑1 (MQ=60)
                                                                     TAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATT                                                                                 <  SRR19973065.2992641/150‑1 (MQ=60)
                                                                     TAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATT                                                                                 <  SRR19973065.2566306/150‑1 (MQ=60)
                                                                      AAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTA                                                                                >  SRR19973065.847490/1‑150 (MQ=60)
                                                                              CTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTT                                                                        <  SRR19973065.1402157/150‑1 (MQ=60)
                                                                              CTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTT                                                                        <  SRR19973065.1763858/150‑1 (MQ=60)
                                                                                   CTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCAT                                                                   <  SRR19973065.367924/150‑1 (MQ=60)
                                                                                   CTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCAT                                                                   <  SRR19973065.2148592/150‑1 (MQ=60)
                                                                                   CTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCAT                                                                   <  SRR19973065.1171647/150‑1 (MQ=60)
                                                                                    TCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATA                                                                  <  SRR19973065.1172117/150‑1 (MQ=60)
                                                                                       TGGCTCTGATTATTCATTTTGACCGTCAACGTTTTTTCATCACACTTGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTC                                                               <  SRR19973065.2244908/150‑1 (MQ=60)
                                                                                       TGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTC                                                               >  SRR19973065.2521741/1‑150 (MQ=60)
                                                                                       TGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTC                                                               >  SRR19973065.2521832/1‑150 (MQ=60)
                                                                                        GGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCA                                                              <  SRR19973065.843991/150‑1 (MQ=60)
                                                                                        GGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCA                                                              <  SRR19973065.843741/150‑1 (MQ=60)
                                                                                           GCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATAT                                                           <  SRR19973065.1831735/150‑1 (MQ=60)
                                                                                               ATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCG                                                       <  SRR19973065.3108014/150‑1 (MQ=60)
                                                                                                    TCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGAC                                                  >  SRR19973065.2079470/1‑150 (MQ=60)
                                                                                                     CAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACT                                                 <  SRR19973065.879818/150‑1 (MQ=60)
                                                                                                     CAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACT                                                 <  SRR19973065.879098/150‑1 (MQ=60)
                                                                                                          TGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCT                                            <  SRR19973065.3070156/150‑1 (MQ=60)
                                                                                                              CGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTC                                        >  SRR19973065.1395961/1‑150 (MQ=60)
                                                                                                                TCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTG                                      <  SRR19973065.1131211/150‑1 (MQ=60)
                                                                                                                TCAACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTG                                      <  SRR19973065.1106705/150‑1 (MQ=60)
                                                                                                                  AACGGTTTTTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGA                                    <  SRR19973065.247999/150‑1 (MQ=60)
                                                                                                                          TTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATA                            <  SRR19973065.761511/150‑1 (MQ=60)
                                                                                                                          TTCATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATA                            >  SRR19973065.231973/1‑150 (MQ=60)
                                                                                                                            CATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACG                          <  SRR19973065.3059535/150‑1 (MQ=60)
                                                                                                                             ATCACACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGC                         >  SRR19973065.1893387/1‑150 (MQ=60)
                                                                                                                                 CACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTC                     >  SRR19973065.2362730/1‑150 (MQ=60)
                                                                                                                                  ACTGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCC                    >  SRR19973065.60278/1‑150 (MQ=60)
                                                                                                                                    TGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCT                  >  SRR19973065.490756/1‑150 (MQ=60)
                                                                                                                                    TGGTATTCCAGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCT                  <  SRR19973065.871344/150‑1 (MQ=60)
                                                                                                                                             AGCGTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCA         >  SRR19973065.2624285/1‑150 (MQ=60)
                                                                                                                                                GTATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGT      <  SRR19973065.1065454/150‑1 (MQ=60)
                                                                                                                                                 TATAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTG     <  SRR19973065.352509/150‑1 (MQ=60)
                                                                                                                                                   TAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGTA   <  SRR19973065.3443373/150‑1 (MQ=60)
                                                                                                                                                   TAGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGTA   <  SRR19973065.3443364/150‑1 (MQ=60)
                                                                                                                                                    AGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGTAA  >  SRR19973065.1897226/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TAGATTCCGTCAGTGTAACGCGCGCCAGAGGCTGAAATGCCCTGTTTGAGATGCAGTAGTTGATTATCGTAAACAAAACTGACCTCCTGGCGCGGATTATTCAGTTTGACCGTCAACGGTTTTTCATCACACTGGTATTCCAGCGTATCGGTTTGCATGCGCTCAACCAGCTGATTAAAGGCGCTACAGCCGGAGAGAAGGACCGGCAAAATGATAATTAACAGTTTTTTCATAGTCATATCCCGAAGACTTTCCTGGTCTGGAGGGCAATACGCCCTCCCTAACGTTCCAAGTGTAA  >  CP009273/1712414‑1712711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑