Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,818,738 C→A 47.7% D153Y (GAC→TAC)  ves ← cold‑ and stress‑inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,818,7380CA47.7% 108.1 / 225.6 180D153Y (GAC→TAC) vescold‑ and stress‑inducible protein
Reads supporting (aligned to +/- strand):  ref base C (53/41);  new base A (38/48);  total (91/89)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

AGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATC  >  CP009273/1818592‑1818882
                                                                                                                                                  |                                                                                                                                                
aGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCg                                                                                                                                                 >  1:2554542/1‑148 (MQ=255)
aGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCg                                                                                                                                                 >  1:2553761/1‑148 (MQ=255)
aGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCg                                                                                                                                                 <  1:85331/148‑1 (MQ=255)
 gCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAgg                                                                                                                                                >  1:3530294/1‑148 (MQ=255)
  ccGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGt                                                                                                                                               <  2:1008573/148‑1 (MQ=255)
  ccGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGt                                                                                                                                               <  2:2952340/148‑1 (MQ=255)
   cGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTa                                                                                                                                              <  1:158884/148‑1 (MQ=255)
      tgctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTg                                                                                                                                           <  2:1855141/148‑1 (MQ=255)
      tgctTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTg                                                                                                                                           <  1:1902395/148‑1 (MQ=255)
           tCCGGTAACTATGAAATTACT‑AACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAAt                                                                                                                                     <  1:1450453/148‑1 (MQ=255)
            ccGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAAt                                                                                                                                     <  2:709947/148‑1 (MQ=255)
              ggTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACATTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAAttt                                                                                                                                   <  1:3113239/148‑1 (MQ=255)
              ggTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAAttt                                                                                                                                   >  1:214314/1‑148 (MQ=255)
              ggTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAAttt                                                                                                                                   <  1:1231512/148‑1 (MQ=255)
               gTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTa                                                                                                                                  >  1:1838195/1‑148 (MQ=255)
                tAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTAt                                                                                                                                 >  2:2639453/1‑148 (MQ=255)
                tAACTATGAAAGTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTAt                                                                                                                                 <  2:2991069/148‑1 (MQ=255)
                 aaCTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATc                                                                                                                                >  1:378913/1‑148 (MQ=255)
                  aCTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCg                                                                                                                               <  2:3097574/148‑1 (MQ=255)
                   cTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGc                                                                                                                              >  2:2580828/1‑148 (MQ=255)
                     aTGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGccc                                                                                                                            >  1:914838/1‑148 (MQ=255)
                          aTTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTg                                                                                                                       >  1:2527840/1‑148 (MQ=255)
                                  aCTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGccc                                                                                                               <  2:496733/148‑1 (MQ=255)
                                    ttGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCg                                                                                                             <  1:3003395/148‑1 (MQ=255)
                                     tGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGt                                                                                                            >  1:2677464/1‑148 (MQ=255)
                                      gATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGtt                                                                                                           <  1:2256210/148‑1 (MQ=255)
                                         ccGGCGATTGACCAGCGAGCCAGTTGATTTCACTGAACATCAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAAt                                                                                                        <  1:2259848/148‑1 (MQ=255)
                                           ggCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATca                                                                                                      <  1:3062103/148‑1 (MQ=255)
                                            gCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATcac                                                                                                     >  2:3347680/1‑148 (MQ=255)
                                            gCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATcac                                                                                                     >  2:1967298/1‑148 (MQ=255)
                                             cgtttGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATcaca                                                                                                    <  1:2577262/144‑1 (MQ=255)
                                              gAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACaa                                                                                                   <  1:1419785/148‑1 (MQ=255)
                                               aaTGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACaaa                                                                                                  <  2:3202625/148‑1 (MQ=255)
                                               aaTGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACaaa                                                                                                  <  2:3172567/148‑1 (MQ=255)
                                                aTGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAac                                                                                                 <  1:3313487/148‑1 (MQ=255)
                                                 tGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAaca                                                                                                >  1:646775/1‑148 (MQ=255)
                                                  gACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAacac                                                                                               >  1:891711/1‑148 (MQ=255)
                                                   aCCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAAcacc                                                                                              <  1:1422440/148‑1 (MQ=255)
                                                     cAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAAcaccac                                                                                            <  1:2305712/148‑1 (MQ=255)
                                                       gCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCAccc                                                                                          >  1:648836/1‑148 (MQ=255)
                                                               gTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGt                                                                                  <  1:215908/148‑1 (MQ=255)
                                                                 tGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGa                                                                                >  2:1923107/1‑148 (MQ=255)
                                                                 tGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGa                                                                                >  2:3164155/1‑148 (MQ=255)
                                                                 tGATTTCAATGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGAACAGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTCATCACAAACACAAACCCCCCGCGTGa                                                                                >  2:3163798/1‑148 (MQ=255)
                                                                   aTTTCACTGAACAACAGTTTACCTTGCGGTTGAAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGc                                                                              <  1:17101/148‑1 (MQ=255)
                                                                    tttAACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGCGTGTGCCTGCCATCGAACCAGCAAGCGCCCTGGTCGGTAGTGAGCAATTTATAGACCAGTTGCCACGCCCCGTTAATCACAAACACCAACCCGCCGCGAGAGcc                                                                             >  2:3421557/1‑148 (MQ=255)
                                                                      tCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCaa                                                                           <  1:1778660/148‑1 (MQ=255)
                                                                         cTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAgg                                                                        >  2:1187281/1‑148 (MQ=255)
                                                                          tGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgt                                                                       >  2:1719781/1‑148 (MQ=255)
                                                                          tGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgt                                                                       >  2:1587301/1‑148 (MQ=255)
                                                                           gAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgta                                                                      >  1:336291/1‑148 (MQ=255)
                                                                           gAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGgta                                                                      >  1:224930/1‑148 (MQ=255)
                                                                              ccacaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGAGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTGCATCACAAACACCACCCCGCCGCGGGAGCCAAAGggagtc                                                                   >  2:266864/3‑147 (MQ=255)
                                                                               aacaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTaa                                                                  >  1:3383884/1‑148 (MQ=255)
                                                                               aacaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTaa                                                                  >  2:1634899/1‑148 (MQ=255)
                                                                               aacaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTaa                                                                  <  2:1327810/148‑1 (MQ=255)
                                                                                acaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTaaa                                                                 >  2:990609/1‑148 (MQ=255)
                                                                                acaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTaaa                                                                 <  2:571630/148‑1 (MQ=255)
                                                                                acaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTaaa                                                                 <  1:1021778/148‑1 (MQ=255)
                                                                                acaGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTaaa                                                                 <  2:2658425/148‑1 (MQ=255)
                                                                                    tttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgta                                                             <  1:2431294/148‑1 (MQ=255)
                                                                                    tttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgta                                                             >  2:3318075/1‑148 (MQ=255)
                                                                                     ttACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgtac                                                            >  1:3400522/1‑148 (MQ=255)
                                                                                      tACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgtacg                                                           <  2:173839/148‑1 (MQ=255)
                                                                                      tACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAacgtacg                                                           <  1:550779/148‑1 (MQ=255)
                                                                                         cTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTc                                                        >  2:1017869/1‑148 (MQ=255)
                                                                                          ttGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCg                                                       <  2:3530294/148‑1 (MQ=255)
                                                                                             cGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCa                                                    <  1:1249678/148‑1 (MQ=255)
                                                                                             cGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCa                                                    >  2:2504426/1‑148 (MQ=255)
                                                                                             cGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCa                                                    <  2:810530/148‑1 (MQ=255)
                                                                                                ttGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAAtt                                                 >  1:2003298/1‑148 (MQ=255)
                                                                                                   tAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTg                                              >  1:936723/1‑148 (MQ=255)
                                                                                                   tAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTg                                              >  1:2422337/1‑148 (MQ=255)
                                                                                                     aaaGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGAc                                            >  1:1712627/1‑148 (MQ=255)
                                                                                                     aaaGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGAc                                            <  2:3452428/148‑1 (MQ=255)
                                                                                                        gCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACttt                                         >  1:3341571/1‑148 (MQ=255)
                                                                                                         ccGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTc                                        >  1:2385583/1‑148 (MQ=255)
                                                                                                              gggTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCtt                                   <  2:2313809/148‑1 (MQ=255)
                                                                                                              gggTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCtt                                   >  2:985041/1‑148 (MQ=255)
                                                                                                                  gtgCCTGCCATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAg                               <  1:2771124/148‑1 (MQ=255)
                                                                                                                     cctgccATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACa                            <  1:2411256/148‑1 (MQ=255)
                                                                                                                      ctgccATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACAt                           <  1:2053227/148‑1 (MQ=255)
                                                                                                                      ctgccATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACAt                           <  2:930497/148‑1 (MQ=255)
                                                                                                                       tgccATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATc                          >  1:1530294/1‑148 (MQ=255)
                                                                                                                       tgccATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATc                          >  2:2214572/1‑148 (MQ=255)
                                                                                                                        gccATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATcc                         <  2:3368917/148‑1 (MQ=255)
                                                                                                                         ccATCGAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCa                        <  1:2419938/148‑1 (MQ=255)
                                                                                                                            tCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGc                     >  2:2242674/1‑148 (MQ=255)
                                                                                                                            tCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGc                     >  2:2013947/1‑148 (MQ=255)
                                                                                                                             cGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCg                    >  2:2757478/1‑148 (MQ=255)
                                                                                                                              gAACCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCgt                   <  1:2461476/148‑1 (MQ=255)
                                                                                                                                aCCAGCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCgtgt                 >  1:571600/1‑148 (MQ=255)
                                                                                                                                  gctcACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAGCACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCa               <  1:1723806/145‑1 (MQ=255)
                                                                                                                                    gCACGCGCCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCatg             >  2:994987/1‑148 (MQ=255)
                                                                                                                                      aCGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCatgat           <  2:1634569/148‑1 (MQ=255)
                                                                                                                                        gcgcCCTGGTAGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGt         <  2:2424783/148‑1 (MQ=255)
                                                                                                                                         cgcCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGtt        >  1:1952456/1‑148 (MQ=255)
                                                                                                                                              tGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCATAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAAt   >  1:2006516/1‑148 (MQ=255)
                                                                                                                                               ggTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATc  <  1:1282562/148‑1 (MQ=255)
                                                                                                                                               ggTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATc  <  1:3057180/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AGCCGCTGCTTTCCGGTAACTATGAAATTACTGAACTTGATCCGGCGAATGACCAGCGAGCCAGTTGATTTCACTGAACAACAGTTTACCTTGCGGTTGTAAAAGCCGCAGGGTGTGCCTGCCATCGAACCAGCACGCGCCCTGGTCGGTAGTGAGCAATTTATCGCCCAGTTGCCACGCCCCGTTAATCACAAACACCACCCCGCCGCGTGAGCCAAAGGTAGTAAACGTACGTTCGGCAATTCTGACTTTCGCCTTACAGACATCCAGGCGTGTCATGATGTTGAAATC  >  CP009273/1818592‑1818882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A