Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F394 I0 R1	1342	215.0	7134094	96.2%	6862998	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,222,656	A→C	30.0%	intergenic (+112/+261)	yohP → / ← dusC	uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,222,656	0	A	C	30.0%	62.2 / 5.4	69	intergenic (+112/+261)	yohP/dusC	uncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand): ref base A (3/28); new base C (4/9); total (13/56)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCC  >  CP009273/2222509‑2222802

cTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCa                                                                                                                                                      <  2:1645275/148‑1 (MQ=255)

    tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAgggg                                                                                                                                                  <  1:3072047/148‑1 (MQ=255)

     ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAggggg                                                                                                                                                 <  1:171843/148‑1 (MQ=255)

        gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggcgg                                                                                                                                              <  1:2127139/148‑1 (MQ=255)

         aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggcggc                                                                                                                                             <  2:796675/148‑1 (MQ=255)

           tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCa                                                                                                                                           <  1:1774754/148‑1 (MQ=255)

            ggCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCaa                                                                                                                                          <  1:143122/148‑1 (MQ=255)

            ggCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCaa                                                                                                                                          <  1:2558651/148‑1 (MQ=255)

             gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑Ggggggggggggggcg                                                                                                                                         >  1:376907/1‑143 (MQ=255)

             gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑Ggggggggggggggca                                                                                                                                         >  1:2173661/1‑143 (MQ=255)

             gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑Gggggggggggggccg                                                                                                                                         >  1:1305148/1‑143 (MQ=255)

             gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑Ggggggggggggcccg                                                                                                                                         >  2:1509586/1‑143 (MQ=255)

               gTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑Tcg                                                                                                                                      <  2:789400/148‑1 (MQ=255)

                tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCaacgc                                                                                                                                       >  2:3085761/1‑145 (MQ=255)

                tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGCGGGGGGGccccaccc                                                                                                                                      >  2:3085999/1‑142 (MQ=255)

                tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑Tcgc                                                                                                                                     <  1:638204/148‑1 (MQ=255)

                 aTTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGcccacgcc                                                                                                                                      >  2:1076352/1‑142 (MQ=255)

                   ttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCa                                                                                                                                           <  1:887022/140‑1 (MQ=255)

                   ttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCa                                                                                                                                           >  2:887022/1‑140 (MQ=255)

                         tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGcccc                                                                                                                            <  1:3156169/148‑1 (MQ=255)

                           atatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCTGGGGGGGGCC‑A‑GAGGCGCCGcccccc                                                                                                                          >  2:2616421/1‑147 (MQ=255)

                              ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGGCCA‑ACGCCCCCCCCCCCCGcc                                                                                                                       >  2:3510468/1‑147 (MQ=255)

                              ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCa                                                                                                                       <  2:96169/148‑1 (MQ=255)

                              ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCa                                                                                                                       <  2:1507388/148‑1 (MQ=255)

                                    aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGGGGGCGC‑CCCCCGCccccccccccccccccc                                                                                                                 >  1:769841/1‑147 (MQ=255)

                                       taattcatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGGCCA‑ACCCCCCCCCCCCCCGCAACCCCCGGc                                                                                                              >  2:586804/1‑148 (MQ=37)

                                       taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCGTGTTGCGCTCCTGTCATGTGCATTGCTTAATATAATCAGTGGCGAATGGCGCGC‑GCTGGGGGGGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGc                                                                                                              <  1:1762710/148‑1 (MQ=37)

                                       taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCCGGGGGGGCCCAA‑AACCCGCC‑CCCCCCCCAcccccccgc                                                                                                              >  2:586936/1‑144 (MQ=255)

                                       taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGGGGGCCA‑ACGCCGCCCCCCCCAGCACCCCCCGGc                                                                                                              >  2:2377490/1‑148 (MQ=37)

                                        aattaatGTCATCAGGTCAGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGct                                                                                                             <  2:447818/148‑1 (MQ=255)

                                          ttaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCCGGGGGGGCCCA‑CCGCCCCCCCCCCCCCCCCCCCCCGGccca                                                                                                           >  2:3194148/1‑145 (MQ=255)

                                           taatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCCA‑AC‑CCGCCCCCCCCCCCCCCCCCCGGcccgg                                                                                                          >  2:1181408/1‑144 (MQ=255)

                                                           gAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCA‑AAGCCCCCCCCCCCGGCACCCCCCGGCGCTGGCCGAACaaaaacccg                                                                                          >  2:2269311/1‑143 (MQ=25)

                                                            aaaaTAACGAGAATTTTTCTGTCTCTCATCGTGTTGCGGTCGTGTCTTGTGCTTTGCTTCATATAATCCGGGGCGCATGGAGCGC‑GCTGGGGGCGGCAAA‑GCGCCGCCGCCCCCTGCACCCCCGGGCTCGGG‑CGAACAAAATcgccgc                                                                                        <  2:2438892/148‑1 (MQ=18)

                                                                tAACGATAATATTTCTGTCTCTCAGCGTGTTGCGCTCCGTTCTTGTGCATTGCTTCATATAATCACGGGCGCATGGAGCGC‑GCTGGGGGCGGCAAA‑TCGCCGCCGCCCCCGGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTgcg                                                                                    <  1:2943635/148‑1 (MQ=18)

                                                                   ctagaTTATTTCAGTCTCTCATCGTTTTGCGCTCCTGTCATGTGAATTGCTTCATATAATCACGGGCGCATGGAGCGC‑GCTGGGGGCGGCCAA‑CCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCgg                                                                                 <  1:550048/146‑1 (MQ=18)

                                                                        ttatTTCAGTCTCTCATCGTGTTGCGCTCGTGACTTGTGCATTGCTACAAATACTCACTGGCGCACGGAGCGC‑GCCGGGGGCGGCAAA‑GCGCCGCCCCCCCGGGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGccc                                                                            <  2:1231759/147‑1 (MQ=17)

                                                                        atttttCGGTCTCTCATCCGGTGGCGCCCCTGTCTTGTGATTGGCTTCATATAACCCCGGGCGCCGGGCGCGC‑GCGGGGGGCGCCCAC‑GCGCCGCCGCCCCCTCCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGccc                                                                            <  1:2880536/145‑1 (MQ=12)

                                                                             tCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                       <  2:2498367/148‑1 (MQ=255)

                                                                                 tctctcTTCCTGTTGCGCTCCTGTCTTGTGCTTTGCTTAATATAATCACGGGCGCACGGCGCGC‑GCGGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                                   <  1:2297444/148‑1 (MQ=18)

                                                                                 tctctcATCCTGTGGCGCTCCTGTAATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                                   <  2:2157372/148‑1 (MQ=255)

                                                                                          ttGTTGCGCTCCTGTCTTTTGATTTTATTAATATAAACCCTGGCGAATGGAGCGC‑GCTGGGGGGGGCAAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                          <  1:1098902/147‑1 (MQ=14)

                                                                                          gtGTTGCGCTCGTGTATGGTGCTTTGCTTCATATACTCCGTGGCGCATGGAGCGC‑GCGGGGGGCGGCAAA‑TCGCCGCCGCCCCCGGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                          <  1:1101035/147‑1 (MQ=14)

                                                                                          cTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACGGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                          <  2:2976614/148‑1 (MQ=255)

                                                                                                   tCCTTTCATGTGATTTGTTTAATATAATCACGGGCCCATGGCGGGC‑GCCGGGGGCGCCAAT‑TCGCCGCCGCCCCCGGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                 <  1:1089472/148‑1 (MQ=14)

                                                                                                   tCCTGTCATGTGCATTGCTTCATATAATCCCGGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                 <  2:1127335/148‑1 (MQ=255)

                                                                                                   gccTGTCTTGTGATTTGCTACATATAATCCCGGGCGCACGGAGCGC‑GCGGGGGGCGCCCAA‑GCGCCGCCGCCCCGGGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                 <  1:3126102/147‑1 (MQ=14)

                                                                                                          aTGTGATTTGCTTCATATAATCACTGGCGCAAGGCGCGC‑GCCGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                          <  1:2061552/148‑1 (MQ=255)

                                                                                                            gtgCTTTGCTTCATATACCCCCGGGCGCACGGCGCGG‑GCGGGGGGCGCCCAA‑GCCCCCCCCCCCCCGCCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                        <  1:344157/148‑1 (MQ=11)

                                                                                                             tgattggCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                       <  2:913748/142‑1 (MQ=255)

                                                                                                             tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                       <  2:1752164/148‑1 (MQ=255)

                                                                                                             tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATAGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                       <  2:208604/148‑1 (MQ=255)

                                                                                                             tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATAGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                       <  2:208479/148‑1 (MQ=255)

                                                                                                             tgCATTGCTTCATATAAGCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATAGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                       <  2:1575436/148‑1 (MQ=255)

                                                                                                              gctttGTTTCATATAACCACGGGCGCAAGGCGCGC‑GCGGGGGGCGGCCAA‑GCGCCGCCGCCCCCGGCACCCCCGGGCTCGGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                      <  1:2539247/145‑1 (MQ=11)

                                                                                                               cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                     <  2:3033949/148‑1 (MQ=255)

                                                                                                               cATTGCTACATATAATCCCGGGCGCACGGAGCGC‑GCTGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGt                                     <  1:573539/148‑1 (MQ=14)

                                                                                                                 ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                   <  2:376907/148‑1 (MQ=255)

                                                                                                                 ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                   <  2:2173661/148‑1 (MQ=255)

                                                                                                                 ttGCTTAATAAAACCACGGGCGCACGGCGCGC‑GCTGGGGGCGGCCAA‑TCGCCGCCGCCCCCTCCACCCCCGGGCTCGGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                   <  1:2498014/148‑1 (MQ=11)

                                                                                                                       cATATACTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATccc                             <  2:3197813/148‑1 (MQ=25)

                                                                                                                             atccggggCGCAGGGCGCGG‑GC‑GGGGGGGGCCAACGCGCCGCGGCCCCGTCCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTaaa                       <  1:2984798/142‑1 (MQ=11)

                                                                                                                                tcTGGCGCAGGGTGGGC‑GCGGGGGGCGGCCAC‑GCGCCGCCGCCCCCGGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                    <  1:934588/147‑1 (MQ=11)

                                                                                                                                  gggCGCATGGCGCGC‑GCGGGGGGGGCCAAA‑GGGCCGCCCCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                  <  1:2992508/147‑1 (MQ=11)

                                                                                                                                    gcgcAAGGAGCGC‑GCCGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                <  2:3068007/148‑1 (MQ=17)

                                                                                                                                     cgcATGGAGCGC‑GCAGGGGGCGGCCAA‑GCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGccc               <  2:1231974/148‑1 (MQ=17)

                                                                                                                                     cgcAAGGAGCGC‑GCTGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGccc               <  2:1231828/148‑1 (MQ=17)

                                                                                                                                      gcAAGGAGCGC‑GCCGGGGGCGGCCAA‑GCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt              <  2:1559211/148‑1 (MQ=17)

                                                                                                                                      gcAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt              <  2:1559105/148‑1 (MQ=32)

                                                                                                                                         aGGCGCGC‑GCCGGGGGCGCCCAA‑GCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATAGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg           <  1:325848/148‑1 (MQ=11)

                                                                                                                                         aGGAGCGC‑GCTGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg           <  1:1477968/148‑1 (MQ=17)

                                                                                                                                          ggAGCGC‑GCTGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc          <  1:3526890/148‑1 (MQ=17)

                                                                                                                                                c‑gcCGGGGGGGGCCAA‑GCGCCGCCGCCCCCGCCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAt    <  1:1277387/148‑1 (MQ=11)

                                                                                                                                                   cAGGGGGCGGCCAC‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc  <  2:68837/148‑1 (MQ=17)

CTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAA‑TCGCCGCCGCCCCCTGCACCCCCGGGCTCTGG‑CGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCC  >  CP009273/2222509‑2222802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A