Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,253,174 T→A 38.7% intergenic (‑399/+24) psuK ← / ← fruA pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,253,1740TA38.7% 179.4 / 259.8 212intergenic (‑399/+24)psuK/fruApseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base T (70/60);  new base A (45/37);  total (115/97)
Fisher's exact test for biased strand distribution p-value = 8.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 216 total aligned reads displayed.

AATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGG  >  CP009273/2253029‑2253320
                                                                                                                                                 |                                                                                                                                                  
aataatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATc                                                                                                                                                  >  2:216068/1‑148 (MQ=255)
aataatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATc                                                                                                                                                  >  2:216146/1‑148 (MQ=255)
  taatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTg                                                                                                                                                >  1:1116551/1‑148 (MQ=255)
  taatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTg                                                                                                                                                >  2:1579115/1‑148 (MQ=255)
    atCTGCCCTTGAGTAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGcc                                                                                                                                              <  2:1929569/148‑1 (MQ=255)
    atCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGcc                                                                                                                                              <  2:2806884/148‑1 (MQ=255)
      cTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCt                                                                                                                                            >  1:3115860/1‑148 (MQ=255)
        gCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCGCAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGt                                                                                                                                          >  2:664958/1‑148 (MQ=255)
            ttGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAaca                                                                                                                                      >  1:3545281/1‑148 (MQ=255)
             tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAacac                                                                                                                                     >  2:2939348/1‑148 (MQ=255)
                 gAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCt                                                                                                                                 >  2:2578802/1‑148 (MQ=255)
                  aaaTAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCtt                                                                                                                                <  1:899023/148‑1 (MQ=255)
                    aTAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGAGAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCtttt                                                                                                                              <  2:3305482/148‑1 (MQ=255)
                      aaCCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTttat                                                                                                                            <  1:2612475/148‑1 (MQ=255)
                          tGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACg                                                                                                                        <  1:308640/148‑1 (MQ=255)
                             gtgtTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACgctg                                                                                                                     <  2:1115068/148‑1 (MQ=255)
                               gtTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACgctgct                                                                                                                   <  1:809504/148‑1 (MQ=255)
                               gtTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACgctgct                                                                                                                   <  1:2452463/148‑1 (MQ=255)
                               gtTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACgctgct                                                                                                                   <  1:1614034/148‑1 (MQ=255)
                                  tgatgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTc                                                                                                                <  1:3099148/148‑1 (MQ=255)
                                  tgatgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTAATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTc                                                                                                                >  2:2437535/1‑148 (MQ=255)
                                     tgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCt                                                                                                             >  2:2641000/1‑148 (MQ=255)
                                     tgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCt                                                                                                             >  2:851905/1‑148 (MQ=255)
                                     tgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCt                                                                                                             >  2:595323/1‑148 (MQ=255)
                                      gaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTa                                                                                                            >  2:1313597/1‑148 (MQ=255)
                                      gaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTa                                                                                                            >  2:1773940/1‑148 (MQ=255)
                                            ttGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTGTCGCTACTGCGt                                                                                                      >  2:363934/1‑148 (MQ=255)
                                             tGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTc                                                                                                     <  2:2533397/148‑1 (MQ=255)
                                                 gTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACt                                                                                                 >  2:1734675/1‑148 (MQ=255)
                                                 gTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACt                                                                                                 >  1:3115269/1‑148 (MQ=255)
                                                  tttCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACtt                                                                                                >  2:470505/1‑148 (MQ=255)
                                                      tACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCgg                                                                                            <  1:470505/148‑1 (MQ=255)
                                                              gATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCa                                                                                    <  1:1245536/148‑1 (MQ=255)
                                                              gATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCa                                                                                    >  1:1483064/1‑148 (MQ=255)
                                                               aTAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAg                                                                                   >  1:732151/1‑148 (MQ=255)
                                                               aTAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAg                                                                                   >  1:2728807/1‑148 (MQ=255)
                                                                 aaaaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGa                                                                                 >  1:2272693/1‑148 (MQ=255)
                                                                  aaaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGaa                                                                                <  1:679072/148‑1 (MQ=255)
                                                                   aaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg                                                                               >  2:1755407/1‑148 (MQ=255)
                                                                   aaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg                                                                               >  2:1756185/1‑148 (MQ=255)
                                                                      cATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCa                                                                            <  2:2915529/148‑1 (MQ=255)
                                                                      cATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCa                                                                            >  1:2024921/1‑148 (MQ=255)
                                                                      cATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCa                                                                            <  1:1674789/148‑1 (MQ=255)
                                                                        tttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATa                                                                          <  1:2596115/148‑1 (MQ=255)
                                                                        tttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATa                                                                          <  2:2285381/148‑1 (MQ=255)
                                                                         ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGACATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAg                                                                         >  2:1272102/1‑148 (MQ=255)
                                                                           aaaTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGc                                                                       <  2:3121288/148‑1 (MQ=255)
                                                                             aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                                     >  2:2244316/1‑148 (MQ=255)
                                                                             aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                                     >  1:1492751/1‑148 (MQ=255)
                                                                             aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                                     >  2:1191235/1‑148 (MQ=255)
                                                                              ttCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaa                                                                    >  2:2985145/1‑148 (MQ=255)
                                                                              ttCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGATTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaa                                                                    >  2:1970014/1‑148 (MQ=255)
                                                                                cATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAc                                                                  <  1:2836061/148‑1 (MQ=255)
                                                                                      aTTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGcca                                                            >  1:1383936/1‑148 (MQ=255)
                                                                                        tACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccacc                                                          >  1:2497214/1‑148 (MQ=255)
                                                                                        tACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccacc                                                          >  2:346073/1‑148 (MQ=255)
                                                                                        tACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccacc                                                          <  2:3505850/148‑1 (MQ=255)
                                                                                           aTTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTTCAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGc                                                       <  1:997442/148‑1 (MQ=255)
                                                                                           aTTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGc                                                       <  1:997362/148‑1 (MQ=255)
                                                                                           aTTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGc                                                       <  1:2641479/148‑1 (MQ=255)
                                                                                            ttCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCg                                                      >  1:545568/1‑148 (MQ=255)
                                                                                             tCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGt                                                     >  1:1165213/1‑148 (MQ=255)
                                                                                             tCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGt                                                     >  2:1682365/1‑148 (MQ=255)
                                                                                             tCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGt                                                     >  1:3360692/1‑148 (MQ=255)
                                                                                                caAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTAcc                                                  >  2:758620/1‑148 (MQ=255)
                                                                                                caAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTAcc                                                  >  2:1870295/1‑148 (MQ=255)
                                                                                                  aaCCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGACAAACCCGCCACCAGCGTACCgg                                                <  1:2802486/148‑1 (MQ=255)
                                                                                                   aCCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGc                                               >  1:1310224/1‑148 (MQ=255)
                                                                                                   aCCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGc                                               >  1:3072869/1‑148 (MQ=255)
                                                                                                      ggTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaat                                            >  1:2802546/1‑148 (MQ=255)
                                                                                                      ggTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaat                                            <  2:2386059/148‑1 (MQ=255)
                                                                                                        tCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGATGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaataa                                          >  2:2013979/1‑148 (MQ=255)
                                                                                                           ttCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTg                                       <  2:3450855/148‑1 (MQ=255)
                                                                                                           ttCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTg                                       <  1:2214799/148‑1 (MQ=255)
                                                                                                           ttCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTg                                       >  2:435188/1‑148 (MQ=255)
                                                                                                               ctTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTAc                                   <  1:293392/148‑1 (MQ=255)
                                                                                                               ctTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTAc                                   <  1:216068/148‑1 (MQ=255)
                                                                                                               ctTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTAc                                   <  1:216146/148‑1 (MQ=255)
                                                                                                                    cgcTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGt                              <  1:576386/148‑1 (MQ=255)
                                                                                                                      cTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTaa                            >  1:3380869/1‑148 (MQ=255)
                                                                                                                       tcatcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAc                           >  1:1690190/1‑148 (MQ=255)
                                                                                                                        catcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAcc                          >  1:3203237/1‑148 (MQ=255)
                                                                                                                         atcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAccc                         >  2:2743534/1‑148 (MQ=255)
                                                                                                                           caAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCCGCGTACCGGCAATCATTGCTACCAGGTAACCCaa                       >  2:2616895/1‑148 (MQ=255)
                                                                                                                           caAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCaa                       <  1:2329432/148‑1 (MQ=255)
                                                                                                                           caAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCaa                       <  1:1328111/148‑1 (MQ=255)
                                                                                                                              aTGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAc                    <  1:1708183/148‑1 (MQ=255)
                                                                                                                               tGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAcc                   >  2:2227782/1‑148 (MQ=255)
                                                                                                                                gTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCg                  >  2:2878096/1‑148 (MQ=255)
                                                                                                                                gTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCg                  <  1:2413119/148‑1 (MQ=255)
                                                                                                                                  tACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc                <  2:3240658/148‑1 (MQ=255)
                                                                                                                                   acaggacaggAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCg               <  2:934885/148‑1 (MQ=255)
                                                                                                                                       gacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAt           <  1:595323/148‑1 (MQ=255)
                                                                                                                                       gacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAt           <  1:851906/148‑1 (MQ=255)
                                                                                                                                        acaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATa          <  2:1442342/148‑1 (MQ=255)
                                                                                                                                           ggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAgcg       >  2:2349235/1‑148 (MQ=255)
                                                                                                                                           ggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAACGGCGTAATAgcg       >  2:2193656/1‑148 (MQ=255)
                                                                                                                                            gaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAgcgc      >  2:561086/1‑148 (MQ=255)
                                                                                                                                             aaaTTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGcc     <  1:1251161/148‑1 (MQ=255)
                                                                                                                                                tttCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAgg  >  2:2010745/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGG  >  CP009273/2253029‑2253320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A