Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,253,174 T→A 94.1% intergenic (‑399/+24) psuK ← / ← fruA pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,253,1740TA94.1% 788.4 / 28.3 251intergenic (‑399/+24)psuK/fruApseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base T (8/7);  new base A (126/110);  total (134/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

AATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCA  >  CP009273/2253029‑2253318
                                                                                                                                                 |                                                                                                                                                
aataatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTc                                                                                                                                                <  2:2391997/148‑1 (MQ=255)
 ataatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGATTGAAGTTTCTACTGAAAGATAAAACCAATTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCt                                                                                                                                               <  1:2700784/148‑1 (MQ=255)
         cccTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTa                                                                                                                                       <  1:2025749/148‑1 (MQ=255)
         cccTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTa                                                                                                                                       <  1:487942/148‑1 (MQ=255)
         cccTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTa                                                                                                                                       <  1:487687/148‑1 (MQ=255)
           cTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAac                                                                                                                                     >  1:1070612/1‑148 (MQ=255)
            ttGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAaca                                                                                                                                    >  2:2102068/1‑148 (MQ=255)
                ggAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACAcc                                                                                                                                <  2:2217894/148‑1 (MQ=255)
                ggAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACAcc                                                                                                                                >  1:3494326/1‑148 (MQ=255)
                ggAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACAcc                                                                                                                                <  1:310472/148‑1 (MQ=255)
                 gAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCt                                                                                                                               >  1:2225285/1‑148 (MQ=255)
                    aTAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCtttt                                                                                                                            <  1:359641/148‑1 (MQ=255)
                         cTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTAc                                                                                                                       >  1:1051202/1‑148 (MQ=255)
                         cTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTAc                                                                                                                       >  1:1139276/1‑148 (MQ=255)
                          tGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACg                                                                                                                      <  1:1251284/148‑1 (MQ=255)
                          tGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACg                                                                                                                      <  1:2068775/148‑1 (MQ=255)
                           gAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACgc                                                                                                                     >  2:907368/1‑148 (MQ=255)
                             gtgtTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACgctg                                                                                                                   <  1:307638/148‑1 (MQ=255)
                               gtTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACgctgct                                                                                                                 <  2:2173456/148‑1 (MQ=255)
                                tttGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCtt                                                                                                                <  1:995628/148‑1 (MQ=255)
                                    atgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGc                                                                                                            <  2:1386919/148‑1 (MQ=255)
                                    atgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGc                                                                                                            <  2:1559002/148‑1 (MQ=255)
                                     tgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCt                                                                                                           <  2:2342190/148‑1 (MQ=255)
                                      gaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTa                                                                                                          <  1:1936355/148‑1 (MQ=255)
                                      gaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTAATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTa                                                                                                          >  2:3071704/1‑148 (MQ=255)
                                         cGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTg                                                                                                       >  2:2639581/1‑148 (MQ=255)
                                            ttGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGt                                                                                                    >  2:3237966/1‑148 (MQ=255)
                                              gAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTcc                                                                                                  <  1:31952/148‑1 (MQ=255)
                                               aaGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCa                                                                                                 >  2:1935326/1‑148 (MQ=255)
                                               aaGTTTCTACTCCCAGATACAACCATTTAAATTCATAAAATTACATTCACCAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTCTTACGCTGCTTTCGCTACTGCGTCCa                                                                                                 <  2:258770/148‑1 (MQ=255)
                                                aGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCAc                                                                                                <  1:3334763/148‑1 (MQ=255)
                                                        cTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGAc                                                                                        >  1:144439/1‑148 (MQ=255)
                                                         tGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACg                                                                                       >  2:1540958/1‑148 (MQ=255)
                                                         tGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACg                                                                                       >  2:1338147/1‑148 (MQ=255)
                                                          gAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGt                                                                                      >  2:1863766/1‑148 (MQ=255)
                                                           aaaGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGtt                                                                                     >  2:489635/1‑148 (MQ=255)
                                                            aaGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGttt                                                                                    >  2:1677784/1‑148 (MQ=255)
                                                              gATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCa                                                                                  >  1:3187306/1‑148 (MQ=255)
                                                              gATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCa                                                                                  >  1:1284623/1‑148 (MQ=255)
                                                               aTAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAg                                                                                 >  2:1111215/1‑148 (MQ=255)
                                                               aTAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAg                                                                                 >  2:2861545/1‑148 (MQ=255)
                                                                tAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAgg                                                                                >  2:682363/1‑148 (MQ=255)
                                                                 aaaaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGa                                                                               >  2:2832697/1‑148 (MQ=255)
                                                                  aaaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGGTTCAGGaa                                                                              >  2:518951/1‑148 (MQ=255)
                                                                   aaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAg                                                                             >  2:714235/1‑148 (MQ=255)
                                                                    aCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAgg                                                                            >  1:1707560/1‑148 (MQ=255)
                                                                       aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCAt                                                                         <  1:1165757/148‑1 (MQ=255)
                                                                       aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCAt                                                                         <  1:2987828/148‑1 (MQ=255)
                                                                       aTTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCAt                                                                         <  2:2480162/148‑1 (MQ=255)
                                                                         ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAg                                                                       >  1:1165904/1‑148 (MQ=255)
                                                                         ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAg                                                                       >  1:2616352/1‑148 (MQ=255)
                                                                             aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                                   >  2:2934973/1‑148 (MQ=255)
                                                                             aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                                   >  2:2937234/1‑148 (MQ=255)
                                                                             aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                                   >  2:2304667/1‑148 (MQ=255)
                                                                             aTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                                   >  1:1000610/1‑148 (MQ=255)
                                                                                 aTAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAcc                                                               <  2:1982734/148‑1 (MQ=255)
                                                                                   aaaaTTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCg                                                             >  2:2766272/1‑148 (MQ=255)
                                                                                   aaaaTTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCg                                                             >  2:842334/1‑148 (MQ=255)
                                                                                    aaaTTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGc                                                            <  2:2756216/148‑1 (MQ=255)
                                                                                      aTTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGcca                                                          >  1:527460/1‑148 (MQ=255)
                                                                                       ttACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccac                                                         >  1:2818268/1‑148 (MQ=255)
                                                                                       ttACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccac                                                         <  2:1074060/148‑1 (MQ=255)
                                                                                        tACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccacc                                                        >  1:2402964/1‑148 (MQ=255)
                                                                                        tACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGccacc                                                        >  1:2045328/1‑148 (MQ=255)
                                                                                          cATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAg                                                      >  2:579962/1‑148 (MQ=255)
                                                                                              cacaAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTa                                                  >  2:1332619/1‑148 (MQ=255)
                                                                                                  aaCCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCgg                                              >  2:2446429/1‑148 (MQ=255)
                                                                                                    ccGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCa                                            >  1:2533966/1‑148 (MQ=255)
                                                                                                      ggTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaat                                          <  1:867840/148‑1 (MQ=255)
                                                                                                             ctctTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCt                                   <  1:293451/148‑1 (MQ=255)
                                                                                                               ctTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTAc                                 >  2:2090280/1‑148 (MQ=255)
                                                                                                                 ttGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCCCCAGCGTACCGGCAATAATTGCTACCa                               >  2:1574096/1‑148 (MQ=255)
                                                                                                                 ttGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCa                               <  2:237192/148‑1 (MQ=255)
                                                                                                                        catcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAcc                        >  1:3294005/1‑148 (MQ=255)
                                                                                                                        catcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAcc                        >  1:973904/1‑148 (MQ=255)
                                                                                                                         atcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAccc                       >  2:1206292/1‑148 (MQ=255)
                                                                                                                          tcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                      <  2:788493/148‑1 (MQ=255)
                                                                                                                          tcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                      <  2:1871836/148‑1 (MQ=255)
                                                                                                                          tcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                      >  2:3083100/1‑148 (MQ=255)
                                                                                                                          tcaAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                      <  1:2575799/148‑1 (MQ=255)
                                                                                                                           caAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCaa                     >  2:1888900/1‑148 (MQ=255)
                                                                                                                             aaTGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATa                   <  1:1677784/148‑1 (MQ=255)
                                                                                                                              aTGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAc                  <  1:2118666/148‑1 (MQ=255)
                                                                                                                               tGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAcc                 <  1:823336/148‑1 (MQ=255)
                                                                                                                                gTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCg                >  1:2374793/1‑148 (MQ=255)
                                                                                                                                gTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTCCTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAAAAATTGCTCCCAGGTCACCCCATACCg                >  2:3181582/1‑148 (MQ=255)
                                                                                                                                 ttACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCgg               <  2:2044817/148‑1 (MQ=255)
                                                                                                                                 ttACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCgg               <  2:438932/148‑1 (MQ=255)
                                                                                                                                   acaggacaggAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCg             >  2:174611/1‑148 (MQ=255)
                                                                                                                                   acaggacaggAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCg             >  1:1554899/1‑148 (MQ=255)
                                                                                                                                     aggacaggaAATATCTGCCCTGTAACACACCTTGTATGACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTa           <  2:1941854/148‑1 (MQ=255)
                                                                                                                                       gacaggaAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAt         <  1:659553/148‑1 (MQ=255)
                                                                                                                                       gacaggaAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAt         >  1:644965/1‑148 (MQ=255)
                                                                                                                                        acaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATa        <  1:2829020/148‑1 (MQ=255)
                                                                                                                                         cgggaAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAg       >  2:1930911/3‑148 (MQ=255)
                                                                                                                                         caggaAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAg       >  1:2191712/1‑148 (MQ=255)
                                                                                                                                           ggaAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAgcg     >  2:3390800/1‑148 (MQ=255)
                                                                                                                                            gaAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAgcgc    <  2:2997871/148‑1 (MQ=255)
                                                                                                                                            gaAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAgcgc    >  1:58702/1‑148 (MQ=255)
                                                                                                                                              aaTATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCa  <  1:1664840/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCA  >  CP009273/2253029‑2253318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGAT  >  CP009273/2253025‑2253323
                                                                                                                                                     |                                                                                                                                                     
AGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATA                                                                                                                                                       >  SRR19973065.238896/1‑150 (MQ=60)
AGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATA                                                                                                                                                       >  SRR19973065.237994/1‑150 (MQ=60)
 GAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATAT                                                                                                                                                      >  SRR19973065.2099725/1‑150 (MQ=60)
 GAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATAT                                                                                                                                                      <  SRR19973065.2138022/150‑1 (MQ=60)
     ATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGATTGAAGTTTCTACTGAAAGATAAAACCAATTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGC                                                                                                                                                  <  SRR19973065.2717564/150‑1 (MQ=60)
      TAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCC                                                                                                                                                 <  SRR19973065.1069878/150‑1 (MQ=60)
        ATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCT                                                                                                                                               <  SRR19973065.1467822/150‑1 (MQ=60)
             CCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAAC                                                                                                                                          <  SRR19973065.2038770/150‑1 (MQ=60)
             CCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAAC                                                                                                                                          <  SRR19973065.490985/150‑1 (MQ=60)
             CCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAAC                                                                                                                                          <  SRR19973065.491177/150‑1 (MQ=60)
             CCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAAC                                                                                                                                          >  SRR19973065.1077697/1‑150 (MQ=60)
             CCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAAC                                                                                                                                          <  SRR19973065.491241/150‑1 (MQ=60)
             CCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAAC                                                                                                                                          <  SRR19973065.491245/150‑1 (MQ=60)
             CCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAAC                                                                                                                                          >  SRR19973065.3016215/1‑150 (MQ=60)
             CCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAAC                                                                                                                                          >  SRR19973065.3016235/1‑150 (MQ=60)
                 TGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACAC                                                                                                                                      <  SRR19973065.1383126/150‑1 (MQ=60)
                  GAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACC                                                                                                                                     >  SRR19973065.3515564/1‑150 (MQ=60)
                  GAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACC                                                                                                                                     >  SRR19973065.562672/1‑150 (MQ=60)
                   AGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCT                                                                                                                                    >  SRR19973065.2239467/1‑150 (MQ=60)
                   AGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCT                                                                                                                                    <  SRR19973065.1711544/150‑1 (MQ=60)
                    GGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTT                                                                                                                                   <  SRR19973065.312489/150‑1 (MQ=60)
                    GGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTT                                                                                                                                   <  SRR19973065.312555/150‑1 (MQ=60)
                    GGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTT                                                                                                                                   <  SRR19973065.312559/150‑1 (MQ=60)
                     GAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTT                                                                                                                                  <  SRR19973065.2073691/150‑1 (MQ=60)
                        ATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTAT                                                                                                                               <  SRR19973065.362169/150‑1 (MQ=60)
                           ACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTAC                                                                                                                            >  SRR19973065.1055869/1‑150 (MQ=60)
                           ACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTAC                                                                                                                            >  SRR19973065.1058165/1‑150 (MQ=60)
                           ACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTAC                                                                                                                            >  SRR19973065.1146649/1‑150 (MQ=60)
                           ACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTAC                                                                                                                            <  SRR19973065.927736/150‑1 (MQ=60)
                            CCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACG                                                                                                                           <  SRR19973065.3504425/150‑1 (MQ=60)
                            CCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACG                                                                                                                           <  SRR19973065.627555/150‑1 (MQ=60)
                            CCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACG                                                                                                                           <  SRR19973065.627571/150‑1 (MQ=60)
                             CTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGC                                                                                                                          >  SRR19973065.1154054/1‑150 (MQ=60)
                             CTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGC                                                                                                                          >  SRR19973065.728059/1‑150 (MQ=60)
                              TGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCT                                                                                                                         <  SRR19973065.2081964/150‑1 (MQ=60)
                              TGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCT                                                                                                                         <  SRR19973065.2081955/150‑1 (MQ=60)
                              TGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCT                                                                                                                         <  SRR19973065.1259522/150‑1 (MQ=60)
                                 GTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCT                                                                                                                      <  SRR19973065.309690/150‑1 (MQ=60)
                                    atctATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTC                                                                                                                   <  SRR19973065.247215/146‑1 (MQ=60)
                                    TTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTC                                                                                                                   <  SRR19973065.376637/150‑1 (MQ=60)
                                    TTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTC                                                                                                                   <  SRR19973065.1002244/150‑1 (MQ=60)
                                      TGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGC                                                                                                                 >  SRR19973065.998093/1‑150 (MQ=60)
                                        ATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTA                                                                                                               >  SRR19973065.2251583/1‑150 (MQ=60)
                                          GAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACT                                                                                                             <  SRR19973065.1949059/150‑1 (MQ=60)
                                          GAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACT                                                                                                             <  SRR19973065.1949031/150‑1 (MQ=60)
                                          GAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACT                                                                                                             <  SRR19973065.163723/150‑1 (MQ=60)
                                               TTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTC                                                                                                        >  SRR19973065.1670011/1‑150 (MQ=60)
                                                  GAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCAC                                                                                                     <  SRR19973065.32351/150‑1 (MQ=60)
                                                  GAAGTTTCTACTGAAAGATAAAACCATATAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCAC                                                                                                     <  SRR19973065.32355/150‑1 (MQ=60)
                                                    AGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTT                                                                                                   <  SRR19973065.3355122/150‑1 (MQ=60)
                                                          TACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGAC                                                                                             >  SRR19973065.145659/1‑150 (MQ=60)
                                                              GAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTT                                                                                         >  SRR19973065.2633671/1‑150 (MQ=60)
                                                              GAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTT                                                                                         >  SRR19973065.867161/1‑150 (MQ=60)
                                                               AAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTC                                                                                        >  SRR19973065.2349665/1‑150 (MQ=60)
                                                                AAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCA                                                                                       >  SRR19973065.3207738/1‑150 (MQ=60)
                                                                AAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCA                                                                                       >  SRR19973065.3206541/1‑150 (MQ=60)
                                                                AAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCA                                                                                       >  SRR19973065.1293097/1‑150 (MQ=60)
                                                                AAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCA                                                                                       >  SRR19973065.1289567/1‑150 (MQ=60)
                                                                      AAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGG                                                                                 >  SRR19973065.1718921/1‑150 (MQ=60)
                                                                         CCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCAT                                                                              >  SRR19973065.1685573/1‑150 (MQ=60)
                                                                           ATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATGTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAG                                                                            <  SRR19973065.1173241/150‑1 (MQ=60)
                                                                           ATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAG                                                                            >  SRR19973065.1173348/1‑150 (MQ=60)
                                                                           ATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAG                                                                            <  SRR19973065.1173201/150‑1 (MQ=60)
                                                                           ATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAG                                                                            >  SRR19973065.1293282/1‑150 (MQ=60)
                                                                           ATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAG                                                                            <  SRR19973065.3006114/150‑1 (MQ=60)
                                                                           ATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAG                                                                            >  SRR19973065.2632562/1‑150 (MQ=60)
                                                                               AAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCA                                                                        >  SRR19973065.2771670/1‑150 (MQ=60)
                                                                               AAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCA                                                                        >  SRR19973065.1007257/1‑150 (MQ=60)
                                                                                  TTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAC                                                                     >  SRR19973065.1150611/1‑150 (MQ=60)
                                                                                   TCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACC                                                                    <  SRR19973065.1086814/150‑1 (MQ=60)
                                                                                        AAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCA                                                               >  SRR19973065.530912/1‑150 (MQ=60)
                                                                                        AAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCA                                                               >  SRR19973065.530919/1‑150 (MQ=60)
                                                                                         AATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCAC                                                              >  SRR19973065.2835685/1‑150 (MQ=60)
                                                                                         AATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCAC                                                              >  SRR19973065.2835680/1‑150 (MQ=60)
                                                                                          ATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACC                                                             >  SRR19973065.2418073/1‑150 (MQ=60)
                                                                                          ATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACC                                                             >  SRR19973065.2058412/1‑150 (MQ=60)
                                                                                          ATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACC                                                             >  SRR19973065.2057768/1‑150 (MQ=60)
                                                                                                      AACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCA                                                 <  SRR19973065.1917353/150‑1 (MQ=60)
                                                                                                      AACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCA                                                 >  SRR19973065.2549868/1‑150 (MQ=60)
                                                                                                          GGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAA                                             <  SRR19973065.873404/150‑1 (MQ=60)
                                                                                                          GGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAA                                             <  SRR19973065.873421/150‑1 (MQ=60)
                                                                                                             CATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTG                                          <  SRR19973065.2000182/150‑1 (MQ=60)
                                                                                                             CATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTG                                          <  SRR19973065.2406590/150‑1 (MQ=60)
                                                                                                                TCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTA                                       <  SRR19973065.1069214/150‑1 (MQ=60)
                                                                                                                 CTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTAC                                      <  SRR19973065.295345/150‑1 (MQ=60)
                                                                                                                   CTTTGCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCA                                    >  SRR19973065.2066652/1‑150 (MQ=60)
                                                                                                                       GCGCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTA                                <  SRR19973065.217506/150‑1 (MQ=60)
                                                                                                                         GCTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAAC                              >  SRR19973065.2594449/1‑150 (MQ=60)
                                                                                                                          CTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACC                             >  SRR19973065.3314065/1‑150 (MQ=60)
                                                                                                                          CTCATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACC                             >  SRR19973065.980373/1‑150 (MQ=60)
                                                                                                                             ATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTTGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTCACCCAA                          >  SRR19973065.1224597/1‑150 (MQ=60)
                                                                                                                             ATCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTTGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAA                          >  SRR19973065.2493912/1‑150 (MQ=60)
                                                                                                                              TCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACTAGCGTACCGGCAATAATTGCTACCAGGTAACCCAAT                         <  SRR19973065.2628309/150‑1 (MQ=60)
                                                                                                                              TCAAATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAAT                         <  SRR19973065.2591863/150‑1 (MQ=60)
                                                                                                                                 AATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACC                      <  SRR19973065.1689002/150‑1 (MQ=60)
                                                                                                                                  ATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCG                     >  SRR19973065.2389616/1‑150 (MQ=60)
                                                                                                                                  ATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCG                     <  SRR19973065.2132299/150‑1 (MQ=60)
                                                                                                                                  ATGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCG                     <  SRR19973065.353414/150‑1 (MQ=60)
                                                                                                                                   TGTTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGG                    <  SRR19973065.828738/150‑1 (MQ=60)
                                                                                                                                     TTACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCG                  >  SRR19973065.1565207/1‑150 (MQ=60)
                                                                                                                                      TACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGT                 <  SRR19973065.3257464/150‑1 (MQ=60)
                                                                                                                                       ACAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTA                >  SRR19973065.289811/1‑150 (MQ=60)
                                                                                                                                       ACAGGACAGGAAATATCTGCCCTGTAACAAACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTAACGGCAATAATTGCTACCAGGTAACCCAATAacggagga                >  SRR19973065.2755468/1‑142 (MQ=60)
                                                                                                                                        CAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAA               <  SRR19973065.2955051/150‑1 (MQ=60)
                                                                                                                                        CAGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAA               <  SRR19973065.3193488/150‑1 (MQ=60)
                                                                                                                                         AGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAT              >  SRR19973065.1202791/1‑150 (MQ=60)
                                                                                                                                         AGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAT              >  SRR19973065.2136264/1‑150 (MQ=60)
                                                                                                                                         AGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAT              >  SRR19973065.3347855/1‑150 (MQ=60)
                                                                                                                                         AGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAT              >  SRR19973065.649203/1‑150 (MQ=60)
                                                                                                                                         AGGACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAT              >  SRR19973065.170924/1‑150 (MQ=60)
                                                                                                                                           GACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAG            <  SRR19973065.663621/150‑1 (MQ=60)
                                                                                                                                           GACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAG            >  SRR19973065.2205785/1‑150 (MQ=60)
                                                                                                                                           GACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAG            >  SRR19973065.2205764/1‑150 (MQ=60)
                                                                                                                                           GACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAG            <  SRR19973065.663913/150‑1 (MQ=60)
                                                                                                                                            ACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGC           <  SRR19973065.2846483/150‑1 (MQ=60)
                                                                                                                                            ACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGC           <  SRR19973065.2846476/150‑1 (MQ=60)
                                                                                                                                            ACAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGC           <  SRR19973065.363105/150‑1 (MQ=60)
                                                                                                                                             CAGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCG          >  SRR19973065.2581844/1‑150 (MQ=60)
                                                                                                                                              AGGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGC         >  SRR19973065.59328/1‑150 (MQ=60)
                                                                                                                                               GGAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCC        >  SRR19973065.810312/1‑150 (MQ=60)
                                                                                                                                                GAAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCA       <  SRR19973065.3483627/150‑1 (MQ=60)
                                                                                                                                                 AAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAG      >  SRR19973065.2239529/1‑150 (MQ=60)
                                                                                                                                                 AAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAG      >  SRR19973065.273252/1‑150 (MQ=60)
                                                                                                                                                 AAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAG      >  SRR19973065.2209812/1‑150 (MQ=60)
                                                                                                                                                 AAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAG      >  SRR19973065.3530908/1‑150 (MQ=60)
                                                                                                                                                 AAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAG      >  SRR19973065.2770859/1‑150 (MQ=60)
                                                                                                                                                 AAATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAG      >  SRR19973065.3530921/1‑150 (MQ=60)
                                                                                                                                                  AATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGG     <  SRR19973065.1675986/150‑1 (MQ=60)
                                                                                                                                                   ATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGG    <  SRR19973065.1947594/150‑1 (MQ=60)
                                                                                                                                                   ATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGG    <  SRR19973065.1947993/150‑1 (MQ=60)
                                                                                                                                                   ATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGG    >  SRR19973065.2951878/1‑150 (MQ=60)
                                                                                                                                                   ATATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGG    >  SRR19973065.2951795/1‑150 (MQ=60)
                                                                                                                                                     ATCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGAT  <  SRR19973065.417668/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCCAGGGAT  >  CP009273/2253025‑2253323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑