Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,456,705 C→A 100% S72Y (TCC→TAC)  yfdF → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,456,7050CA97.2% 882.9 / 13.8 282S72Y (TCC→TAC) yfdFuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (135/139);  total (139/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 282 total aligned reads displayed.

GATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAA  >  CP009273/2456560‑2456849
                                                                                                                                                 |                                                                                                                                                
gatgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACt                                                                                                                                                <  2:1433940/148‑1 (MQ=255)
gatgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACt                                                                                                                                                <  2:1077642/148‑1 (MQ=255)
   gaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAg                                                                                                                                             >  2:2743094/1‑148 (MQ=255)
   gaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAg                                                                                                                                             <  2:154961/148‑1 (MQ=255)
    aaGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGt                                                                                                                                            <  1:1604051/148‑1 (MQ=255)
      gTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCTGTTa                                                                                                                                          >  2:1833337/1‑148 (MQ=255)
        tAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACt                                                                                                                                        >  1:465610/1‑148 (MQ=255)
         aaTGTATTATTACAAGAGTTCAAAAACCTTTTTAATGTTAAGGAAGTAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACtt                                                                                                                                       <  1:95079/148‑1 (MQ=255)
           tGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTtat                                                                                                                                     <  1:3308340/148‑1 (MQ=255)
             gATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAACAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGAGCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatat                                                                                                                                   >  2:1853167/1‑148 (MQ=255)
               ttAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattg                                                                                                                                 >  2:2717836/1‑148 (MQ=255)
               ttAGAACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattg                                                                                                                                 <  1:2554374/148‑1 (MQ=255)
                tAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattga                                                                                                                                >  1:1259492/1‑148 (MQ=255)
                tAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattga                                                                                                                                >  1:3036170/1‑148 (MQ=255)
                 aGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgat                                                                                                                               <  2:73266/148‑1 (MQ=255)
                   tACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatat                                                                                                                             >  1:874885/1‑148 (MQ=255)
                     cAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatattg                                                                                                                           <  1:2858026/148‑1 (MQ=255)
                        gagTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACt                                                                                                                        >  2:3025932/1‑148 (MQ=255)
                        gagTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACt                                                                                                                        >  1:1056184/1‑148 (MQ=255)
                         agTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTc                                                                                                                       <  2:288081/148‑1 (MQ=255)
                           ttCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCa                                                                                                                     >  1:1674534/1‑148 (MQ=255)
                           ttCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCa                                                                                                                     <  2:2643353/148‑1 (MQ=255)
                            tCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCaa                                                                                                                    <  1:2345737/148‑1 (MQ=255)
                               aaaaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGa                                                                                                                 >  1:88622/1‑148 (MQ=255)
                                aaaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGAt                                                                                                                >  1:2233269/1‑148 (MQ=255)
                                    cTTTTTAATGGTAAGGAAGGAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAg                                                                                                            <  1:2010203/148‑1 (MQ=255)
                                      ttttAATTGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGAc                                                                                                          <  2:2036282/148‑1 (MQ=255)
                                      ttttAATGGTAAGGAATGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGAc                                                                                                          <  2:144683/148‑1 (MQ=255)
                                      ttttAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGAc                                                                                                          <  2:2473372/148‑1 (MQ=255)
                                       tttAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGAcc                                                                                                         <  1:2980659/148‑1 (MQ=255)
                                           aTGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCatat                                                                                                     >  2:1315359/1‑148 (MQ=255)
                                             ggTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTa                                                                                                   >  1:3087159/1‑148 (MQ=255)
                                              gTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTAc                                                                                                  >  2:1382251/1‑148 (MQ=255)
                                                aaggaatgAATAAGCACCTGTATTAAACATATACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTa                                                                                                <  1:1646243/148‑1 (MQ=255)
                                                   gaaggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTg                                                                                             >  1:27179/1‑148 (MQ=255)
                                                   gaaggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTg                                                                                             >  1:300253/1‑148 (MQ=255)
                                                   gaaggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTg                                                                                             <  2:3455455/148‑1 (MQ=255)
                                                    aaggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTgg                                                                                            <  1:1007594/148‑1 (MQ=255)
                                                     aggaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGt                                                                                           <  1:130595/148‑1 (MQ=255)
                                                       gaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAt                                                                                         <  1:1576427/148‑1 (MQ=255)
                                                       gaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAt                                                                                         <  2:2392651/148‑1 (MQ=255)
                                                       gaaTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAt                                                                                         >  1:2271274/1‑148 (MQ=255)
                                                       gaaTAAGCACCTGTATTAAACATCTACCTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAt                                                                                         >  2:515363/1‑148 (MQ=255)
                                                          tAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGa                                                                                      >  1:850775/1‑148 (MQ=255)
                                                                ccTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCa                                                                                >  1:2404753/1‑148 (MQ=255)
                                                                 cTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCaa                                                                               <  2:1021424/148‑1 (MQ=255)
                                                                 cTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCaa                                                                               <  1:2803290/148‑1 (MQ=255)
                                                                   gTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGa                                                                             >  2:2357495/1‑148 (MQ=255)
                                                                     aTTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAc                                                                           >  1:2803117/1‑148 (MQ=255)
                                                                     aTTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAc                                                                           <  2:3430328/148‑1 (MQ=255)
                                                                        aaaCATCTACTTGAGCTTATAAAAAACGCCAGACGAGTAAACCACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCaa                                                                        <  2:2168726/148‑1 (MQ=255)
                                                                             tCTAATTGAGATTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTAATTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGaa                                                                   <  1:3362063/148‑1 (MQ=255)
                                                                              cTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAAt                                                                  <  2:3319475/148‑1 (MQ=255)
                                                                                      gCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCg                                                          <  2:3285721/148‑1 (MQ=255)
                                                                                        ttATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGaa                                                        <  1:1461527/148‑1 (MQ=255)
                                                                                          ataAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACt                                                      >  2:2985713/1‑148 (MQ=255)
                                                                                          ataAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACt                                                      >  2:382331/1‑148 (MQ=255)
                                                                                          ataAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTAGTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACt                                                      <  1:2171121/148‑1 (MQ=255)
                                                                                           taAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACta                                                     <  1:1137510/148‑1 (MQ=255)
                                                                                              aaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATaa                                                  <  2:482706/148‑1 (MQ=255)
                                                                                              aaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATaa                                                  <  1:1871232/148‑1 (MQ=255)
                                                                                                 aCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGa                                               >  2:1937272/1‑148 (MQ=255)
                                                                                                    ccATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACaa                                            >  2:1103668/1‑148 (MQ=255)
                                                                                                    ccATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTAACTTATATTGATATTGACTCCAATTATACAGACCATATTACTATTGGTATCTACAACCAAGAACCAATAGAATTACCTGCTAACTATAAAGACaa                                            >  1:3232715/1‑148 (MQ=255)
                                                                                                      aTACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAg                                          >  1:731173/1‑148 (MQ=255)
                                                                                                       tACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGa                                         <  2:3466012/148‑1 (MQ=255)
                                                                                                       tACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGa                                         <  1:2912653/148‑1 (MQ=255)
                                                                                                        aCGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGaa                                        >  1:801108/1‑148 (MQ=255)
                                                                                                         cGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAAc                                       >  2:2843466/1‑148 (MQ=255)
                                                                                                         cGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAAc                                       >  2:3221078/1‑148 (MQ=255)
                                                                                                          gAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACt                                      >  1:1931601/1‑148 (MQ=255)
                                                                                                             tAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGt                                   <  1:1090413/148‑1 (MQ=255)
                                                                                                              aaaCGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTc                                  >  1:2472908/1‑148 (MQ=255)
                                                                                                                 cgacgaTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGt                               >  2:1204000/1‑148 (MQ=255)
                                                                                                                 cgacgaTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGt                               >  1:1724929/1‑148 (MQ=255)
                                                                                                                   acgaTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTAc                             >  2:2276876/1‑148 (MQ=255)
                                                                                                                      aTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACt                                      >  1:2382252/1‑136 (MQ=255)
                                                                                                                       tCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATc                         >  2:1525188/1‑148 (MQ=255)
                                                                                                                       tCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATc                         >  1:1973563/1‑148 (MQ=255)
                                                                                                                         cTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCAt                       <  1:3470530/148‑1 (MQ=255)
                                                                                                                         cTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCAt                       <  2:1259492/148‑1 (MQ=255)
                                                                                                                         cTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCAt                       <  2:3036170/148‑1 (MQ=255)
                                                                                                                          ttATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCAtt                      <  1:1220827/148‑1 (MQ=255)
                                                                                                                           tataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTa                     >  2:1762729/1‑148 (MQ=255)
                                                                                                                            ataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTaa                    <  2:1056184/148‑1 (MQ=255)
                                                                                                                              aGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATg                  >  2:644285/1‑148 (MQ=255)
                                                                                                                              aGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATg                  >  2:926064/1‑148 (MQ=255)
                                                                                                                                ttttATTATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAAACAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGac                <  2:3094504/147‑1 (MQ=255)
                                                                                                                                  ttaatattaataATTACTCAGTTACTTATATTGATTTTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGacaa              <  2:2614997/148‑1 (MQ=255)
                                                                                                                                  ttaatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGacaa              <  1:515363/148‑1 (MQ=255)
                                                                                                                                   taatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGacaac             <  1:2128449/148‑1 (MQ=255)
                                                                                                                                     atattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAt           >  1:3571/1‑148 (MQ=255)
                                                                                                                                      tattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATa          <  1:813919/148‑1 (MQ=255)
                                                                                                                                       attaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAg         >  1:554794/1‑148 (MQ=255)
                                                                                                                                        ttaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTCTCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGt        <  1:3154758/148‑1 (MQ=255)
                                                                                                                                        ttaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGt        <  1:587313/148‑1 (MQ=255)
                                                                                                                                         taataaTTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGtt       >  2:848431/1‑148 (MQ=255)
                                                                                                                                            taatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgag    >  2:2191546/1‑148 (MQ=255)
                                                                                                                                             aatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgaga   <  2:2161565/148‑1 (MQ=255)
                                                                                                                                              atTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGagaa  >  1:3122915/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAA  >  CP009273/2456560‑2456849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGACT  >  CP009273/2456556‑2456853
                                                                                                                                                     |                                                                                                                                                    
CAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTGATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTA                                                                                                                                                      >  SRR19973066.3466621/1‑150 (MQ=60)
 AATTATGAAGTTAATGTATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCC                                                                                                                                                     <  SRR19973066.826834/150‑1 (MQ=60)
 AATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTAC                                                                                                                                                     >  SRR19973066.3505757/1‑150 (MQ=60)
  ATGATGAATTTAATGTATTAGTACAAGAGTTCAAAAACATTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACT                                                                                                                                                    <  SRR19973066.737208/150‑1 (MQ=60)
  ATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACT                                                                                                                                                    >  SRR19973066.2632668/1‑150 (MQ=60)
   ttattattttaatgttttattacaagatttaaaaaacttttttattgtttaggaagtaataagcacatttattaaaaattttgttttGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTC                                                                                                                                                   <  SRR19973066.3068725/63‑1 (MQ=60)
      TGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGT                                                                                                                                                <  SRR19973066.850893/150‑1 (MQ=60)
       GAAGTTAATGTATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTT                                                                                                                                               <  SRR19973066.3159111/150‑1 (MQ=60)
       GAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTT                                                                                                                                               <  SRR19973066.3156566/150‑1 (MQ=60)
       GAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTT                                                                                                                                               >  SRR19973066.980639/1‑150 (MQ=60)
        AAGTTAATGTATTAGTACAAGAGTTCAAAAACCTTTTTATTGTTAAGGAAGGAATACGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTA                                                                                                                                              <  SRR19973066.1615700/150‑1 (MQ=60)
        AAGTTAATGTATTAGTACAAGAGTTAAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTA                                                                                                                                              <  SRR19973066.1615696/150‑1 (MQ=60)
        AAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTA                                                                                                                                              >  SRR19973066.392224/1‑150 (MQ=60)
          GTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACT                                                                                                                                            >  SRR19973066.469159/1‑150 (MQ=60)
            TAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTA                                                                                                                                          >  SRR19973066.2563197/1‑150 (MQ=60)
             AATGTATTATTACAAGAGTTCAAAAACCTTTTTAATGTTAAGGAAGTAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTAT                                                                                                                                         <  SRR19973066.96101/150‑1 (MQ=60)
              ATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATA                                                                                                                                        <  SRR19973066.2392228/150‑1 (MQ=60)
              ATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATA                                                                                                                                        >  SRR19973066.3307360/1‑150 (MQ=60)
               TGGATTATTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATAT                                                                                                                                       <  SRR19973066.1696438/150‑1 (MQ=60)
               TGGATTATTACAAGAGTTAAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATAT                                                                                                                                       <  SRR19973066.3330260/150‑1 (MQ=60)
                GGTTTAGTAAAAGATTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATT                                                                                                                                      <  SRR19973066.401013/150‑1 (MQ=60)
                 GATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTG                                                                                                                                     >  SRR19973066.486330/1‑150 (MQ=60)
                 GATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTG                                                                                                                                     >  SRR19973066.2907076/1‑150 (MQ=60)
                  ATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGA                                                                                                                                    >  SRR19973066.3056461/1‑150 (MQ=60)
                  ATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGA                                                                                                                                    >  SRR19973066.3056256/1‑150 (MQ=60)
                  ATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGA                                                                                                                                    >  SRR19973066.1268575/1‑150 (MQ=60)
                  ATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGA                                                                                                                                    >  SRR19973066.1268518/1‑150 (MQ=60)
                   TTAGAACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGAT                                                                                                                                   <  SRR19973066.2571721/150‑1 (MQ=60)
                    TAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATA                                                                                                                                  <  SRR19973066.1119696/150‑1 (MQ=60)
                     AGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATAT                                                                                                                                 >  SRR19973066.881136/1‑150 (MQ=60)
                     AGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATAT                                                                                                                                 >  SRR19973066.881083/1‑150 (MQ=60)
                     AGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATAT                                                                                                                                 >  SRR19973066.881048/1‑150 (MQ=60)
                         CAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGAC                                                                                                                             <  SRR19973066.2877221/150‑1 (MQ=60)
                          AAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACT                                                                                                                            >  SRR19973066.1063927/1‑150 (MQ=60)
                          AAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACT                                                                                                                            >  SRR19973066.1063945/1‑150 (MQ=60)
                            GAGTAAAAAAACATTTTTAATGGTAAGGAAGGAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCC                                                                                                                          <  SRR19973066.1906990/150‑1 (MQ=60)
                             AGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCA                                                                                                                         >  SRR19973066.1686737/1‑150 (MQ=60)
                               TACAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTCCTTATATTGATATTGACTCCAAT                                                                                                                       <  SRR19973066.1628906/150‑1 (MQ=60)
                                TCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATG                                                                                                                      <  SRR19973066.2361693/150‑1 (MQ=60)
                                 CAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGA                                                                                                                     >  SRR19973066.89586/1‑150 (MQ=60)
                                 CAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGA                                                                                                                     <  SRR19973066.2997529/150‑1 (MQ=60)
                                  AAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGAT                                                                                                                    >  SRR19973066.2248683/1‑150 (MQ=60)
                                  AAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTAATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGAT                                                                                                                    >  SRR19973066.2248899/1‑150 (MQ=60)
                                    AAACCTTTTTATTGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGATTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATAC                                                                                                                  <  SRR19973066.525481/150‑1 (MQ=60)
                                    AAACCTTTTTAATGGTAAGGAAGTAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATAC                                                                                                                  <  SRR19973066.210433/150‑1 (MQ=60)
                                    AAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATAC                                                                                                                  <  SRR19973066.526290/150‑1 (MQ=60)
                                    AAACATTTTTAATGTTAAGCAAGTAATAACAACCTTTATTAAACATCTACTTTAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATAC                                                                                                                  <  SRR19973066.1522250/150‑1 (MQ=60)
                                    AAACATTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATAC                                                                                                                  <  SRR19973066.2023558/150‑1 (MQ=60)
                                        CTTTTTAATGGTAAGGAAGGAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGAC                                                                                                              <  SRR19973066.2024293/150‑1 (MQ=60)
                                         TTTTTAATGTTAAGGAATTAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACC                                                                                                             <  SRR19973066.3013024/150‑1 (MQ=60)
                                           TTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCAT                                                                                                           <  SRR19973066.3000341/150‑1 (MQ=60)
                                            TTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATTATACAGACCATA                                                                                                          >  SRR19973066.1947592/1‑150 (MQ=60)
                                            TTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATA                                                                                                          >  SRR19973066.800838/1‑150 (MQ=60)
                                            TTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATA                                                                                                          >  SRR19973066.800860/1‑150 (MQ=60)
                                             TAATGTTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATAT                                                                                                         <  SRR19973066.2852290/150‑1 (MQ=60)
                                             TAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGATTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATAT                                                                                                         <  SRR19973066.2847715/150‑1 (MQ=60)
                                              AATGGTAAGGAAGTAATAAGCACCTTTATTAAACATCTACTTGAGATTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATT                                                                                                        <  SRR19973066.2738841/150‑1 (MQ=60)
                                               ATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTA                                                                                                       >  SRR19973066.3107786/1‑150 (MQ=60)
                                                    AAGGAATGAATAAGCACCTGTATTAAACATATACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATT                                                                                                  <  SRR19973066.1658231/150‑1 (MQ=60)
                                                    AAGGAAGTAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATT                                                                                                  <  SRR19973066.2443453/150‑1 (MQ=60)
                                                    AAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATT                                                                                                  >  SRR19973066.2561086/1‑150 (MQ=60)
                                                     AGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTG                                                                                                 >  SRR19973066.27430/1‑150 (MQ=60)
                                                     AGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTG                                                                                                 >  SRR19973066.27566/1‑150 (MQ=60)
                                                     AGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTG                                                                                                 >  SRR19973066.302368/1‑150 (MQ=60)
                                                       GAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGT                                                                                               >  SRR19973066.3039869/1‑150 (MQ=60)
                                                       GAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGT                                                                                               >  SRR19973066.892325/1‑150 (MQ=60)
                                                        AAGTAATAAGCACCTGTATTAAAATTCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTA                                                                                              <  SRR19973066.1015013/150‑1 (MQ=60)
                                                         AGTAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAT                                                                                             <  SRR19973066.131890/150‑1 (MQ=60)
                                                         AGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAT                                                                                             >  SRR19973066.2405002/1‑150 (MQ=60)
                                                         AGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAT                                                                                             <  SRR19973066.131877/150‑1 (MQ=60)
                                                         AGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAT                                                                                             >  SRR19973066.2286887/1‑150 (MQ=60)
                                                         AGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTAT                                                                                             >  SRR19973066.2286866/1‑150 (MQ=60)
                                                          GTAATAAGCACCTTTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATC                                                                                            <  SRR19973066.1270189/150‑1 (MQ=60)
                                                           GAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCG                                                                                           <  SRR19973066.1587793/150‑1 (MQ=60)
                                                            AATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGA                                                                                          >  SRR19973066.856929/1‑150 (MQ=60)
                                                             ATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGAC                                                                                         >  SRR19973066.289069/1‑150 (MQ=60)
                                                                  CACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCA                                                                                    >  SRR19973066.2421011/1‑150 (MQ=60)
                                                                  CACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCA                                                                                    >  SRR19973066.3246017/1‑150 (MQ=60)
                                                                   acctttataaaaaatctaatttagaTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAA                                                                                   <  SRR19973066.1248188/125‑1 (MQ=60)
                                                                   ACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAA                                                                                   <  SRR19973066.706543/150‑1 (MQ=60)
                                                                     CTGTATTAAACATCTACTTGAGATTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGA                                                                                 <  SRR19973066.2822156/150‑1 (MQ=60)
                                                                      TGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAA                                                                                >  SRR19973066.845286/1‑150 (MQ=60)
                                                                       GTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAC                                                                               >  SRR19973066.2821349/1‑150 (MQ=60)
                                                                       GTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAC                                                                               >  SRR19973066.2821983/1‑150 (MQ=60)
                                                                       GTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAC                                                                               >  SRR19973066.2822050/1‑150 (MQ=60)
                                                                       GTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAAC                                                                               >  SRR19973066.1598948/1‑150 (MQ=60)
                                                                         ATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCA                                                                             <  SRR19973066.122033/150‑1 (MQ=60)
                                                                               CATCTACTAGAGCTTATAAAAAACGCAATACGAGTAAACGACGATCCTAATAGATTTAAAATTAAAAATTACTCAGTTACTTATATTGATATTGACTCAAATGAAACAGACCATATTACTATAGGTATCTACAACAAACAACCAATAGAA                                                                       >  SRR19973066.3115172/1‑150 (MQ=60)
                                                                                 TCTAATTGAGATTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTAATTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATT                                                                     <  SRR19973066.3384478/150‑1 (MQ=60)
                                                                                  CTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTA                                                                    >  SRR19973066.3489178/1‑150 (MQ=60)
                                                                                       TGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGC                                                               <  SRR19973066.1064841/150‑1 (MQ=60)
                                                                                        GAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCG                                                              <  SRR19973066.3254811/150‑1 (MQ=60)
                                                                                           CTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAAC                                                           >  SRR19973066.3004616/1‑150 (MQ=60)
                                                                                            TTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACT                                                          <  SRR19973066.3253460/150‑1 (MQ=60)
                                                                                            TTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACT                                                          <  SRR19973066.1471865/150‑1 (MQ=60)
                                                                                             TATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTA                                                         >  SRR19973066.2176583/1‑150 (MQ=60)
                                                                                              ATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTAGTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTAT                                                        <  SRR19973066.2186197/150‑1 (MQ=60)
                                                                                               TAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATA                                                       <  SRR19973066.1145653/150‑1 (MQ=60)
                                                                                                  AAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAG                                                    <  SRR19973066.1884594/150‑1 (MQ=60)
                                                                                                     ACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACA                                                 >  SRR19973066.2983012/1‑150 (MQ=60)
                                                                                                     ACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACA                                                 >  SRR19973066.1195051/1‑150 (MQ=60)
                                                                                                      CGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAA                                                >  SRR19973066.1600612/1‑150 (MQ=60)
                                                                                                      CGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTAACTTATATTGATATTGACTCCAATTATACAGACCATATTACTATTGGTATCTACAACCAAGAACCAATAGAATTACCTGCTAACTATAAAGACAA                                                >  SRR19973066.3254066/1‑150 (MQ=60)
                                                                                                       GCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAA                                               >  SRR19973066.3112432/1‑150 (MQ=60)
                                                                                                        CCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAG                                              >  SRR19973066.736594/1‑150 (MQ=60)
                                                                                                        CCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAG                                              >  SRR19973066.735703/1‑150 (MQ=60)
                                                                                                          ATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAA                                            >  SRR19973066.806998/1‑150 (MQ=60)
                                                                                                          ATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAA                                            >  SRR19973066.1790172/1‑150 (MQ=60)
                                                                                                          ATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAA                                            >  SRR19973066.807287/1‑150 (MQ=60)
                                                                                                          ATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAA                                            >  SRR19973066.807297/1‑150 (MQ=60)
                                                                                                           TACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAAC                                           <  SRR19973066.2932089/150‑1 (MQ=60)
                                                                                                            ACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACT                                          >  SRR19973066.1945315/1‑150 (MQ=60)
                                                                                                            ACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACT                                          <  SRR19973066.1093327/150‑1 (MQ=60)
                                                                                                             CGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTC                                         >  SRR19973066.3567512/1‑150 (MQ=60)
                                                                                                              GAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCG                                        <  SRR19973066.907408/150‑1 (MQ=60)
                                                                                                                GTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTC                                      >  SRR19973066.2489771/1‑150 (MQ=60)
                                                                                                                GTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTC                                      >  SRR19973066.1667120/1‑150 (MQ=60)
                                                                                                                GTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTC                                      >  SRR19973066.2489755/1‑150 (MQ=60)
                                                                                                                GTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTC                                      >  SRR19973066.1663295/1‑150 (MQ=60)
                                                                                                                 TAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCC                                     <  SRR19973066.1098359/150‑1 (MQ=60)
                                                                                                                   AACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGT                                   >  SRR19973066.1737409/1‑150 (MQ=60)
                                                                                                                      GACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACT                                <  SRR19973066.2306330/150‑1 (MQ=60)
                                                                                                                        CGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGagatcggaag                              >  SRR19973066.2398317/1‑140 (MQ=60)
                                                                                                                        CGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTAT                              >  SRR19973066.2320581/1‑150 (MQ=60)
                                                                                                                        CGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTAT                              <  SRR19973066.3373040/150‑1 (MQ=60)
                                                                                                                        CGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTAT                              >  SRR19973066.1996541/1‑150 (MQ=60)
                                                                                                                        CGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTAT                              >  SRR19973066.572280/1‑150 (MQ=60)
                                                                                                                         GATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATC                             >  SRR19973066.1987511/1‑150 (MQ=60)
                                                                                                                             CTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTA                         >  SRR19973066.3569426/1‑150 (MQ=60)
                                                                                                                             CTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTA                         <  SRR19973066.3493716/150‑1 (MQ=60)
                                                                                                                              TTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAA                        <  SRR19973066.1229449/150‑1 (MQ=60)
                                                                                                                                   GATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACA                   >  SRR19973066.1753589/1‑150 (MQ=60)
                                                                                                                                     TTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAAC                 >  SRR19973066.3399937/1‑150 (MQ=60)
                                                                                                                                      TTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACA                <  SRR19973066.990220/150‑1 (MQ=60)
                                                                                                                                      TTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACA                <  SRR19973066.1502766/150‑1 (MQ=60)
                                                                                                                                      TTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACA                <  SRR19973066.519165/150‑1 (MQ=60)
                                                                                                                                       TAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAT               >  SRR19973066.3625/1‑150 (MQ=60)
                                                                                                                                       TAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAT               >  SRR19973066.91634/1‑150 (MQ=60)
                                                                                                                                       TAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAT               >  SRR19973066.3120/1‑150 (MQ=60)
                                                                                                                                       TAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACAT               <  SRR19973066.2143196/150‑1 (MQ=60)
                                                                                                                                        AATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATA              <  SRR19973066.2217711/150‑1 (MQ=60)
                                                                                                                                        AATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATA              >  SRR19973066.133748/1‑150 (MQ=60)
                                                                                                                                        AATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATA              <  SRR19973066.2217596/150‑1 (MQ=60)
                                                                                                                                         ATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAG             >  SRR19973066.558773/1‑150 (MQ=60)
                                                                                                                                          TATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGT            <  SRR19973066.819886/150‑1 (MQ=60)
                                                                                                                                           ATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTT           <  SRR19973066.1908346/150‑1 (MQ=60)
                                                                                                                                            TTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTCTCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTG          <  SRR19973066.3175790/150‑1 (MQ=60)
                                                                                                                                            TTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTG          <  SRR19973066.591409/150‑1 (MQ=60)
                                                                                                                                            TTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTG          <  SRR19973066.2398207/150‑1 (MQ=60)
                                                                                                                                               ATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGA       >  SRR19973066.1108864/1‑150 (MQ=60)
                                                                                                                                                TAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAA      >  SRR19973066.3143773/1‑150 (MQ=60)
                                                                                                                                                TAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAA      >  SRR19973066.4862/1‑150 (MQ=60)
                                                                                                                                                 AATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAG     <  SRR19973066.1987371/150‑1 (MQ=60)
                                                                                                                                                 AATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAG     <  SRR19973066.2154463/150‑1 (MQ=60)
                                                                                                                                                   TTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGAC   <  SRR19973066.2389014/150‑1 (MQ=60)
                                                                                                                                                   TTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGAC   <  SRR19973066.2389027/150‑1 (MQ=60)
                                                                                                                                                    TACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGACT  >  SRR19973066.1884317/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CAATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGACT  >  CP009273/2456556‑2456853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑