Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,735,293 A→T 5.7% I20I (ATA→ATT)  yfiR → putative periplasmic inhibitor of YfiN activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,735,2930AT 5.7% 807.8 / 30.0 262I20I (ATA→ATT) yfiRputative periplasmic inhibitor of YfiN activity
Reads supporting (aligned to +/- strand):  ref base A (118/129);  new base T (7/8);  total (125/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 263 total aligned reads displayed.

GACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGCCCTACAAGAGAAT  >  CP009273/2735149‑2735440
                                                                                                                                                |                                                                                                                                                   
 aCGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTTAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCg                                                                                                                                                 <  1:1016456/148‑1 (MQ=255)
     tCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCa                                                                                                                                             <  1:452581/148‑1 (MQ=255)
      cGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAg                                                                                                                                            >  1:929201/1‑148 (MQ=255)
      cGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCACTGAATGCGTTTTTCTCACCGACTGGTCTTACTTCTGATTCTCCTGCTGACGGGAGCAGCAATACTCGCTCAg                                                                                                                                            >  1:2712216/1‑148 (MQ=255)
         aTGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAg                                                                                                                                         <  2:1234488/148‑1 (MQ=255)
           gAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGcc                                                                                                                                       <  2:2730271/148‑1 (MQ=255)
             aaaaaTGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCtt                                                                                                                                     <  2:2367292/148‑1 (MQ=255)
               aaaTGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCt                                                                                                                                   >  1:421792/1‑148 (MQ=255)
                  tGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGAc                                                                                                                                >  1:3212830/1‑148 (MQ=255)
                   gATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACg                                                                                                                               <  1:2341374/148‑1 (MQ=255)
                     tatTTTAATGATCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGtt                                                                                                                             <  1:3360093/148‑1 (MQ=255)
                        tttAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCg                                                                                                                          >  1:375088/1‑148 (MQ=255)
                          tAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGaa                                                                                                                        <  1:1661615/148‑1 (MQ=255)
                          tAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGaa                                                                                                                        >  1:2380864/1‑148 (MQ=255)
                           aaTGAGCATTCTGAAAAAATATCTATGCATTTCGAGCGAATATGGTGGGGATCACTGAATGCGTTTTTCTCACCGACAGTTATTACATCTGATTCTCCTGCTGAAGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGATGCGaaa                                                                                                                       >  2:3388524/1‑148 (MQ=255)
                            aTGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGaaaa                                                                                                                      >  2:1762414/1‑148 (MQ=255)
                            aTGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGaaaa                                                                                                                      <  1:2004370/148‑1 (MQ=255)
                            aTGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGaaaa                                                                                                                      <  2:1529440/148‑1 (MQ=255)
                              gAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAAc                                                                                                                    >  2:1369275/1‑148 (MQ=255)
                              gAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAAc                                                                                                                    <  2:2070116/148‑1 (MQ=255)
                                 cATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTg                                                                                                                 <  2:3063312/148‑1 (MQ=255)
                                    tCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGt                                                                                                              >  2:1924318/1‑148 (MQ=255)
                                     cTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTa                                                                                                             >  2:2104519/1‑148 (MQ=255)
                                       gAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTatg                                                                                                           <  2:2986678/148‑1 (MQ=255)
                                       gAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTatg                                                                                                           <  2:3376896/148‑1 (MQ=255)
                                       gAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTatg                                                                                                           >  1:349791/1‑148 (MQ=255)
                                           aaaTATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATgg                                                                                                       >  2:2153847/1‑148 (MQ=255)
                                            aaTATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGt                                                                                                      <  2:2507659/148‑1 (MQ=255)
                                            aaTATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGt                                                                                                      <  2:1980492/148‑1 (MQ=255)
                                              tatCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGttt                                                                                                    >  2:1414369/1‑148 (MQ=255)
                                               atCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTc                                                                                                   <  2:830689/148‑1 (MQ=255)
                                                      cATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTAtt                                                                                            >  1:3203242/1‑148 (MQ=255)
                                                        tttCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGt                                                                                          >  2:2258115/1‑148 (MQ=255)
                                                        tttCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGt                                                                                          >  2:2473873/1‑148 (MQ=255)
                                                         ttCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTc                                                                                         >  2:231016/1‑148 (MQ=255)
                                                          tCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCa                                                                                        <  2:2809351/148‑1 (MQ=255)
                                                             aGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGtt                                                                                     <  1:2474311/148‑1 (MQ=255)
                                                               cGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTac                                                                                   <  2:407500/148‑1 (MQ=255)
                                                               cGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTac                                                                                   >  1:3013780/1‑148 (MQ=255)
                                                               cGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTac                                                                                   >  1:1552928/1‑148 (MQ=255)
                                                                gAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTaca                                                                                  <  2:3279559/148‑1 (MQ=255)
                                                                 aaGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATTCTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTacac                                                                                 <  2:860414/148‑1 (MQ=255)
                                                                 aaGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTacac                                                                                 <  1:1547626/148‑1 (MQ=255)
                                                                   gATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACAccc                                                                               >  2:2785825/1‑148 (MQ=255)
                                                                         ggggATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGc                                                                         <  2:274485/148‑1 (MQ=255)
                                                                          gggATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGcc                                                                        <  2:3409570/148‑1 (MQ=255)
                                                                                 cTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTa                                                                 <  2:732665/148‑1 (MQ=255)
                                                                                 cTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTa                                                                 <  2:1872987/148‑1 (MQ=255)
                                                                                 cTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTa                                                                 <  1:183654/148‑1 (MQ=255)
                                                                                  tGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTAt                                                                <  2:855780/148‑1 (MQ=255)
                                                                                      tGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAgg                                                            <  2:2826185/148‑1 (MQ=255)
                                                                                      tGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAgg                                                            <  1:1378090/148‑1 (MQ=255)
                                                                                      tGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAgg                                                            <  2:255313/148‑1 (MQ=255)
                                                                                          tttttCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGcca                                                        <  1:3241437/148‑1 (MQ=255)
                                                                                           ttttCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATTCTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGccac                                                       >  1:29229/1‑148 (MQ=255)
                                                                                           ttttCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGccac                                                       >  1:3292594/1‑148 (MQ=255)
                                                                                              tctcACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATTCTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCaa                                                    <  2:1293846/148‑1 (MQ=255)
                                                                                              tctcACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCaa                                                    <  2:2914093/148‑1 (MQ=255)
                                                                                               ctcACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCaaa                                                   <  2:1195462/148‑1 (MQ=255)
                                                                                               ctcACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCaaa                                                   <  2:3116717/148‑1 (MQ=255)
                                                                                               ctcACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCaaa                                                   >  1:1153953/1‑148 (MQ=255)
                                                                                               ctcACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGCTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCaaa                                                   >  1:2931166/1‑148 (MQ=255)
                                                                                                   ccGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATTCTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTa                                               >  1:1625643/1‑148 (MQ=255)
                                                                                                   ccGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATTCTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTa                                               >  1:3143917/1‑148 (MQ=255)
                                                                                                   ccGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTa                                               <  2:480327/148‑1 (MQ=255)
                                                                                                    cGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATTCTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTAt                                              <  2:3186814/148‑1 (MQ=255)
                                                                                                    cGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTAt                                              >  1:238969/1‑148 (MQ=255)
                                                                                                    cGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTAt                                              >  1:2547908/1‑148 (MQ=255)
                                                                                                       cTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTa                                           >  2:653011/1‑148 (MQ=255)
                                                                                                       cTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTa                                           >  2:906959/1‑148 (MQ=255)
                                                                                                           tCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCtt                                       <  1:2159068/148‑1 (MQ=255)
                                                                                                            cTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCttt                                      <  1:2831163/148‑1 (MQ=255)
                                                                                                              tACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTc                                    <  2:1360929/148‑1 (MQ=255)
                                                                                                                cTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTTCACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCAt                                  <  1:751812/148‑1 (MQ=255)
                                                                                                                 ttCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATc                                 >  1:1069880/1‑148 (MQ=255)
                                                                                                                 ttCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATc                                 >  1:1740512/1‑148 (MQ=255)
                                                                                                                 ttCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATc                                 <  1:2431591/148‑1 (MQ=255)
                                                                                                                  tCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCt                                >  1:1524171/1‑148 (MQ=255)
                                                                                                                     gATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCa                             <  2:444525/148‑1 (MQ=255)
                                                                                                                     gATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCa                             <  1:25226/148‑1 (MQ=255)
                                                                                                                     gATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTGTCATCTTCa                             >  1:590758/1‑148 (MQ=255)
                                                                                                                      aTTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCAc                            >  1:3108461/1‑148 (MQ=255)
                                                                                                                        tctcCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGt                          >  2:1725993/1‑148 (MQ=255)
                                                                                                                          tcCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGttt                        <  1:3069909/148‑1 (MQ=255)
                                                                                                                          tcCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGttt                        <  2:1965523/148‑1 (MQ=255)
                                                                                                                           ccTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGtttt                       <  1:2815944/148‑1 (MQ=255)
                                                                                                                           ccTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGtttt                       >  1:1376842/1‑148 (MQ=255)
                                                                                                                              gctgACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGt                    <  2:3301327/148‑1 (MQ=255)
                                                                                                                               ctgACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTa                   >  1:1317653/1‑148 (MQ=255)
                                                                                                                                  aCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCg                >  1:997738/1‑148 (MQ=255)
                                                                                                                                  aCGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCg                >  1:3484691/1‑148 (MQ=255)
                                                                                                                                    gggAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGcc              <  2:1135878/148‑1 (MQ=255)
                                                                                                                                     ggAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGccc             >  2:1536979/1‑148 (MQ=255)
                                                                                                                                          aCCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGCCCTACaa        <  2:2593536/148‑1 (MQ=255)
                                                                                                                                           ccAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGCCCTACAag       >  1:868676/1‑148 (MQ=255)
                                                                                                                                             aaTACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGCCCTACAagag     <  2:37914/148‑1 (MQ=255)
                                                                                                                                             aaTACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGCCCTACAagag     <  2:608527/148‑1 (MQ=255)
                                                                                                                                              aTACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGCCCTACAAgaga    >  2:161595/1‑148 (MQ=255)
                                                                                                                                               tACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACTTGCGTATGATGGTTTCCGGTATTTTCAGATACACCCGCTGGCCTGCTCTATCAGTGCCACCAAAGTTAAGTATCTTTTCATCTTCAAGTTTTAGTACCGCCCTACAAGAGaa   >  1:1200883/1‑148 (MQ=255)
                                                                                                                                               tACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGCCCTACAAGAGaa   <  2:3013780/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GACGGTCGCATGAAAAAATGATATTTTAATGAGCATTCTGAAAAAATATCGATGCATTTCGAGCGAAGATGGTGGGGATCCCTGAATGCGTTTTTCTCACCGACTGTTCTTACTTCTGATTCTCCTGCTGACGGGAGCACCAATACTCGCTCAGGAGCCTTCTGACGTTGCGAAAAACGTGCGTATGATGGTTTCCGGTATTGTCAGTTACACCCGCTGGCCTGCGCTATCAGGGCCACCAAAGTTATGTATCTTTTCATCTTCACGTTTTAGTACCGCCCTACAAGAGAAT  >  CP009273/2735149‑2735440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A