Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,736,714 C→A 87.4% R325S (CGC→AGC)  yfiN → putative membrane‑anchored diguanylate cyclase with an N‑terminal periplasmic domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,736,7140CA87.4% 518.2 / 73.9 215R325S (CGC→AGC) yfiNputative membrane‑anchored diguanylate cyclase with an N‑terminal periplasmic domain
Reads supporting (aligned to +/- strand):  ref base C (15/12);  new base A (95/93);  total (110/105)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 217 total aligned reads displayed.

CGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT  >  CP009273/2736570‑2736860
                                                                                                                                                |                                                                                                                                                  
      aCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATAAAAAAAAGATAACTGGGGTCATGCGACGGGCGATAGAGTCTTTATTGAAATCGCAAAACGGTTAGCTGAATTTTGCGGGCTGCGACATAAAGCAAAACGCCTGggcg                                                                                                                                           >  1:2702026/1‑148 (MQ=255)
           ggCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGAt                                                                                                                                      <  2:1153731/148‑1 (MQ=255)
            gCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATg                                                                                                                                     <  1:405509/148‑1 (MQ=255)
              gTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGaa                                                                                                                                   <  2:1824440/148‑1 (MQ=255)
                tACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAtt                                                                                                                                 >  1:1926429/1‑148 (MQ=255)
                tACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAtt                                                                                                                                 >  2:127187/1‑148 (MQ=255)
                 aCTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTc                                                                                                                                >  1:2464203/1‑148 (MQ=255)
                  cTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCg                                                                                                                               >  2:2819432/1‑148 (MQ=255)
                   tATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGc                                                                                                                              <  2:945740/148‑1 (MQ=255)
                    aTTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCt                                                                                                                             <  2:193597/148‑1 (MQ=255)
                        cTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATgg                                                                                                                         <  2:529897/148‑1 (MQ=255)
                        cTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATgg                                                                                                                         >  1:2494438/1‑148 (MQ=255)
                          tGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTg                                                                                                                       <  1:2337361/148‑1 (MQ=255)
                            aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                     <  2:2702026/148‑1 (MQ=255)
                              ggCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGctct                                                                                                                   >  2:1794241/1‑148 (MQ=255)
                              ggCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGctct                                                                                                                   >  1:2290973/1‑148 (MQ=255)
                                   tAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgat                                                                                                              <  2:3099058/148‑1 (MQ=255)
                                   tAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgat                                                                                                              >  2:1542812/1‑148 (MQ=255)
                                   tAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgat                                                                                                              <  1:393342/148‑1 (MQ=255)
                                   tAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgat                                                                                                              >  2:770186/1‑148 (MQ=255)
                                   tAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgat                                                                                                              <  1:1039115/148‑1 (MQ=255)
                                    aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgatg                                                                                                             >  2:179837/1‑148 (MQ=255)
                                    aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgatg                                                                                                             <  2:3148304/148‑1 (MQ=255)
                                     aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGt                                                                                                            >  1:169402/1‑148 (MQ=255)
                                     aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGt                                                                                                            <  2:2201149/148‑1 (MQ=255)
                                     aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGt                                                                                                            <  1:2741089/148‑1 (MQ=255)
                                      tttCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTa                                                                                                           <  1:2218069/148‑1 (MQ=255)
                                      tttCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTa                                                                                                           <  2:2385469/148‑1 (MQ=255)
                                             tACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCgg                                                                                                    >  2:2875119/1‑148 (MQ=255)
                                               cATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGaa                                                                                                  >  2:2268477/1‑148 (MQ=255)
                                                aTCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAt                                                                                                 <  2:420602/148‑1 (MQ=255)
                                                aTCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAt                                                                                                 <  1:710180/148‑1 (MQ=255)
                                                 tCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATc                                                                                                >  1:1552959/1‑148 (MQ=255)
                                                  cAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCt                                                                                               >  1:1108453/1‑148 (MQ=255)
                                                  cAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCt                                                                                               >  2:1334770/1‑148 (MQ=255)
                                                  cAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCt                                                                                               >  2:537894/1‑148 (MQ=255)
                                                   aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTg                                                                                              >  1:1534320/1‑148 (MQ=255)
                                                   aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTg                                                                                              <  2:1094373/148‑1 (MQ=255)
                                                    aTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGa                                                                                             <  1:92045/148‑1 (MQ=255)
                                                    aTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGa                                                                                             <  1:2963862/148‑1 (MQ=255)
                                                     tGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGaa                                                                                            >  1:304637/1‑148 (MQ=255)
                                                     tGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGaa                                                                                            <  2:1049992/148‑1 (MQ=255)
                                                       aTACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGt                                                                                          >  2:2332938/1‑148 (MQ=255)
                                                            tGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgcagc                                                                                     >  2:1455042/1‑148 (MQ=255)
                                                              gggTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGcagcag                                                                                   <  1:357869/148‑1 (MQ=255)
                                                                gTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGat                                                                                 <  1:3295205/148‑1 (MQ=255)
                                                                 tCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGata                                                                                <  1:2064744/148‑1 (MQ=255)
                                                                 tCATGCGACGGGCGATAGAGTCTTGATCGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGata                                                                                <  2:2516263/148‑1 (MQ=255)
                                                                               aTAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                  >  1:1002237/1‑148 (MQ=255)
                                                                               aTAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                  >  2:338827/1‑148 (MQ=255)
                                                                               aTAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGATCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                  >  2:2048587/1‑148 (MQ=255)
                                                                                 agagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGacac                                                                <  2:1152957/148‑1 (MQ=255)
                                                                                 agagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGacac                                                                >  2:246166/1‑148 (MQ=255)
                                                                                   agTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACaa                                                              <  1:2341971/148‑1 (MQ=255)
                                                                                   agTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGAGACaa                                                              >  2:3274331/1‑148 (MQ=255)
                                                                                   agTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACaa                                                              >  1:911420/1‑148 (MQ=255)
                                                                                    gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACaaa                                                             <  2:465348/148‑1 (MQ=255)
                                                                                      cTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATc                                                           >  2:677740/1‑148 (MQ=255)
                                                                                      cTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATc                                                           >  2:902587/1‑148 (MQ=255)
                                                                                      cTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATc                                                           <  1:1167972/148‑1 (MQ=255)
                                                                                      cTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATc                                                           >  1:7622/1‑148 (MQ=255)
                                                                                       ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCt                                                          <  1:575178/148‑1 (MQ=255)
                                                                                        tgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCtt                                                         >  2:694821/1‑148 (MQ=255)
                                                                                          attgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTa                                                       >  2:2311971/1‑148 (MQ=255)
                                                                                          attgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTa                                                       <  1:1276018/148‑1 (MQ=255)
                                                                                            tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGACTCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAt                                                     >  2:2148930/1‑148 (MQ=255)
                                                                                            tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAt                                                     <  2:754214/148‑1 (MQ=255)
                                                                                               aaTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAtctc                                                  <  1:560782/148‑1 (MQ=255)
                                                                                               aaTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAtctc                                                  <  1:1794241/148‑1 (MQ=255)
                                                                                                   gCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGt                                              <  1:179837/148‑1 (MQ=255)
                                                                                                   gCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGt                                              <  1:127187/148‑1 (MQ=255)
                                                                                                   gCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGt                                              <  1:1943963/148‑1 (MQ=255)
                                                                                                    cAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGtt                                             >  1:2956484/1‑148 (MQ=255)
                                                                                                     aaaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGttt                                            >  1:369178/1‑148 (MQ=255)
                                                                                                      aaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTg                                           >  2:424821/1‑148 (MQ=255)
                                                                                                      aaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTg                                           >  2:2141757/1‑148 (MQ=255)
                                                                                                      aaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTg                                           >  2:690517/1‑148 (MQ=255)
                                                                                                        aCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAt                                         <  1:1551414/148‑1 (MQ=255)
                                                                                                         cGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATc                                        >  2:2944298/1‑148 (MQ=255)
                                                                                                           gTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCtt                                      <  2:911983/148‑1 (MQ=255)
                                                                                                           gTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCtt                                      <  2:640895/148‑1 (MQ=255)
                                                                                                            ttAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTc                                     <  1:2374312/148‑1 (MQ=255)
                                                                                                            ttAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTc                                     <  2:2579754/148‑1 (MQ=255)
                                                                                                             tAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCa                                    >  1:1587104/1‑148 (MQ=255)
                                                                                                                   aaTTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATgg                              <  2:1224968/148‑1 (MQ=255)
                                                                                                                     tttGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGcc                            <  1:2149064/148‑1 (MQ=255)
                                                                                                                               tGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGaccac                  <  2:2409535/148‑1 (MQ=255)
                                                                                                                                 cGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGAccacca                >  2:57630/1‑148 (MQ=255)
                                                                                                                                   aCATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATg              <  2:2499935/148‑1 (MQ=255)
                                                                                                                                   aCATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATg              >  2:910663/1‑148 (MQ=255)
                                                                                                                                     aTAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGAc            >  2:60110/1‑148 (MQ=255)
                                                                                                                                       aaaGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAt          >  2:679389/1‑148 (MQ=255)
                                                                                                                                       aaaGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAt          >  1:1371783/1‑148 (MQ=255)
                                                                                                                                       aaaGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAt          >  1:2542327/1‑148 (MQ=255)
                                                                                                                                         aGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTa        >  2:354232/1‑148 (MQ=255)
                                                                                                                                         aGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTa        <  2:136979/148‑1 (MQ=255)
                                                                                                                                          gCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTaa       >  2:490338/1‑148 (MQ=255)
                                                                                                                                          gCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTaa       >  2:723292/1‑148 (MQ=255)
                                                                                                                                           cATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAg      >  2:1680103/1‑148 (MQ=255)
                                                                                                                                               ccGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCAtt  >  2:1193417/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT  >  CP009273/2736570‑2736860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGGT  >  CP009273/2736574‑2736863
                                                                                                                                            |                                                                                                                                                     
AAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATAAAAAAAAGATAACTGGGGTCATGCGACGGGCGATAGAGTCTTTATTGAAATCGCAAAACGGTTAGCTGAATTTTGCGGGCTGCGACATAAAGCAAAACGCCTGGGCG                                                                                                                                              >  SRR19973070.2719503/1‑150 (MQ=60)
        GCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAA                                                                                                                                      <  SRR19973070.408485/150‑1 (MQ=60)
         CGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAT                                                                                                                                     <  SRR19973070.3090321/150‑1 (MQ=60)
          GTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATT                                                                                                                                    >  SRR19973070.1939412/1‑150 (MQ=60)
           TTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTC                                                                                                                                   >  SRR19973070.2480286/1‑150 (MQ=60)
              CTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCT                                                                                                                                >  SRR19973070.382021/1‑150 (MQ=60)
               TATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTA                                                                                                                               >  SRR19973070.2996426/1‑150 (MQ=60)
                 TTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATG                                                                                                                             >  SRR19973070.2425416/1‑150 (MQ=60)
                  TTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGG                                                                                                                            >  SRR19973070.2510617/1‑150 (MQ=60)
                      TGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCT                                                                                                                        <  SRR19973070.2352938/150‑1 (MQ=60)
                      TGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCT                                                                                                                        <  SRR19973070.2352872/150‑1 (MQ=60)
                       GATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTC                                                                                                                       <  SRR19973070.2832675/150‑1 (MQ=60)
                        ATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCT                                                                                                                      >  SRR19973070.1173058/1‑150 (MQ=60)
                        ATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCT                                                                                                                      >  SRR19973070.2306204/1‑150 (MQ=60)
                               TAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGT                                                                                                               <  SRR19973070.1046454/150‑1 (MQ=60)
                               TAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGT                                                                                                               <  SRR19973070.1046322/150‑1 (MQ=60)
                               TAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGT                                                                                                               <  SRR19973070.396380/150‑1 (MQ=60)
                               TAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGT                                                                                                               <  SRR19973070.396228/150‑1 (MQ=60)
                               TAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGT                                                                                                               >  SRR19973070.170772/1‑150 (MQ=60)
                                 ATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTAC                                                                                                             <  SRR19973070.2758724/150‑1 (MQ=60)
                                  TTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACA                                                                                                            <  SRR19973070.2232641/150‑1 (MQ=60)
                                      AAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCG                                                                                                        >  SRR19973070.1542261/1‑150 (MQ=60)
                                        ATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGA                                                                                                      >  SRR19973070.52554/1‑150 (MQ=60)
                                           CATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATC                                                                                                   >  SRR19973070.1563881/1‑150 (MQ=60)
                                           CATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATC                                                                                                   >  SRR19973070.1563886/1‑150 (MQ=60)
                                            ATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCT                                                                                                  >  SRR19973070.1116043/1‑150 (MQ=60)
                                            ATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCT                                                                                                  <  SRR19973070.715243/150‑1 (MQ=60)
                                             TCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTG                                                                                                 >  SRR19973070.1545099/1‑150 (MQ=60)
                                               AATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAA                                                                                               >  SRR19973070.306790/1‑150 (MQ=60)
                                                ATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAG                                                                                              <  SRR19973070.2982055/150‑1 (MQ=60)
                                                ATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAG                                                                                              <  SRR19973070.2983602/150‑1 (MQ=60)
                                                ATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAG                                                                                              <  SRR19973070.92957/150‑1 (MQ=60)
                                                ATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAG                                                                                              <  SRR19973070.2982919/150‑1 (MQ=60)
                                                     ACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAG                                                                                         >  SRR19973070.937078/1‑150 (MQ=60)
                                                     ACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAG                                                                                         >  SRR19973070.937041/1‑150 (MQ=60)
                                                      CCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGC                                                                                        >  SRR19973070.2557990/1‑150 (MQ=60)
                                                      CCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGC                                                                                        >  SRR19973070.2167216/1‑150 (MQ=60)
                                                         GGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGA                                                                                     >  SRR19973070.348538/1‑150 (MQ=60)
                                                         GGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGA                                                                                     >  SRR19973070.365672/1‑150 (MQ=60)
                                                          GGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGAT                                                                                    <  SRR19973070.360482/150‑1 (MQ=60)
                                                           GGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATA                                                                                   <  SRR19973070.1074602/150‑1 (MQ=60)
                                                            GTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATAT                                                                                  <  SRR19973070.3316329/150‑1 (MQ=60)
                                                            GTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATAT                                                                                  <  SRR19973070.565071/150‑1 (MQ=60)
                                                             TCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATG                                                                                 <  SRR19973070.2077774/150‑1 (MQ=60)
                                                             TCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATG                                                                                 <  SRR19973070.2078671/150‑1 (MQ=60)
                                                              CATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGT                                                                                >  SRR19973070.2317790/1‑150 (MQ=60)
                                                              CATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGT                                                                                >  SRR19973070.2227435/1‑150 (MQ=60)
                                                              CATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGT                                                                                <  SRR19973070.1955035/150‑1 (MQ=60)
                                                                         CGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGAC                                                                     >  SRR19973070.1009378/1‑150 (MQ=60)
                                                                             AGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAA                                                                 >  SRR19973070.917901/1‑150 (MQ=60)
                                                                             AGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAA                                                                 >  SRR19973070.918472/1‑150 (MQ=60)
                                                                               AGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAAT                                                               <  SRR19973070.2357508/150‑1 (MQ=60)
                                                                                GTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATC                                                              >  SRR19973070.7731/1‑150 (MQ=60)
                                                                                 TCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCT                                                             <  SRR19973070.1553654/150‑1 (MQ=60)
                                                                                  CTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTT                                                            <  SRR19973070.1176149/150‑1 (MQ=60)
                                                                                  CTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTT                                                            >  SRR19973070.741951/1‑150 (MQ=60)
                                                                                   TTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTT                                                           <  SRR19973070.579113/150‑1 (MQ=60)
                                                                                    TGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTA                                                          <  SRR19973070.599716/150‑1 (MQ=60)
                                                                                     GATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAA                                                         >  SRR19973070.2246827/1‑150 (MQ=60)
                                                                                      ATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAT                                                        <  SRR19973070.1284940/150‑1 (MQ=60)
                                                                                           AATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCC                                                   <  SRR19973070.564485/150‑1 (MQ=60)
                                                                                           AATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCC                                                   <  SRR19973070.1806635/150‑1 (MQ=60)
                                                                                            ATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCG                                                  >  SRR19973070.2676820/1‑150 (MQ=60)
                                                                                              CGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTT                                                >  SRR19973070.2975509/1‑150 (MQ=60)
                                                                                               GCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTT                                               >  SRR19973070.371890/1‑150 (MQ=60)
                                                                                               GCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTT                                               <  SRR19973070.181260/150‑1 (MQ=60)
                                                                                               GCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTT                                               <  SRR19973070.181074/150‑1 (MQ=60)
                                                                                               GCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTT                                               <  SRR19973070.128305/150‑1 (MQ=60)
                                                                                               GCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTT                                               <  SRR19973070.128347/150‑1 (MQ=60)
                                                                                               GCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTT                                               <  SRR19973070.1957021/150‑1 (MQ=60)
                                                                                                  AAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAT                                            >  SRR19973070.1786214/1‑150 (MQ=60)
                                                                                                  AAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGAGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTCACACAAATCTTTAAACTCCCGTTTGAT                                            >  SRR19973070.1641898/1‑150 (MQ=60)
                                                                                                   AACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATC                                           <  SRR19973070.1832688/150‑1 (MQ=60)
                                                                                                    ACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCT                                          <  SRR19973070.1562323/150‑1 (MQ=60)
                                                                                                    ACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCT                                          <  SRR19973070.1197955/150‑1 (MQ=60)
                                                                                                    ACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCT                                          >  SRR19973070.2385023/1‑150 (MQ=60)
                                                                                                       GTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCA                                       >  SRR19973070.1598210/1‑150 (MQ=60)
                                                                                                       GTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCA                                       >  SRR19973070.1597671/1‑150 (MQ=60)
                                                                                                        TTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCAT                                      <  SRR19973070.2390009/150‑1 (MQ=60)
                                                                                                           GCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAAT                                   >  SRR19973070.1774004/1‑150 (MQ=60)
                                                                                                             TGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGG                                 <  SRR19973070.2723177/150‑1 (MQ=60)
                                                                                                                 TTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCAT                             <  SRR19973070.2163104/150‑1 (MQ=60)
                                                                                                                 TTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCAT                             <  SRR19973070.2163377/150‑1 (MQ=60)
                                                                                                                               ACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGAC               <  SRR19973070.248798/150‑1 (MQ=60)
                                                                                                                               ACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGAC               <  SRR19973070.248718/150‑1 (MQ=60)
                                                                                                                                CATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACA              >  SRR19973070.42393/1‑150 (MQ=60)
                                                                                                                                 ATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAT             >  SRR19973070.1381111/1‑150 (MQ=60)
                                                                                                                                 ATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAT             >  SRR19973070.2558952/1‑150 (MQ=60)
                                                                                                                                  TAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATT            <  SRR19973070.773112/150‑1 (MQ=60)
                                                                                                                                      GCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGC        <  SRR19973070.1365614/150‑1 (MQ=60)
                                                                                                                                      GCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGC        <  SRR19973070.1365743/150‑1 (MQ=60)
                                                                                                                                          ACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTG    >  SRR19973070.452026/1‑150 (MQ=60)
                                                                                                                                            AGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGGT  <  SRR19973070.1005655/150‑1 (MQ=60)
                                                                                                                                            AGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGGT  <  SRR19973070.1005948/150‑1 (MQ=60)
                                                                                                                                            |                                                                                                                                                     
AAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGGT  >  CP009273/2736574‑2736863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑