Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,736,714 C→A 100% R325S (CGC→AGC)  yfiN → putative membrane‑anchored diguanylate cyclase with an N‑terminal periplasmic domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,736,7140CA97.6% 700.9 / 4.3 220R325S (CGC→AGC) yfiNputative membrane‑anchored diguanylate cyclase with an N‑terminal periplasmic domain
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (110/104);  total (115/105)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

CCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTG  >  CP009273/2736569‑2736861
                                                                                                                                                 |                                                                                                                                                   
ccGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGc                                                                                                                                                   <  2:3382799/148‑1 (MQ=255)
 cGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGcc                                                                                                                                                  >  2:2478380/1‑148 (MQ=255)
  gAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCt                                                                                                                                                 >  2:1107242/1‑148 (MQ=255)
  gAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCt                                                                                                                                                 >  2:3022621/1‑148 (MQ=255)
  gAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCGTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCt                                                                                                                                                 >  2:2857960/1‑148 (MQ=255)
   aaaaaCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTg                                                                                                                                                <  2:2854138/148‑1 (MQ=255)
     aaaCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTggg                                                                                                                                              <  1:2485773/148‑1 (MQ=255)
        cGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGggcgg                                                                                                                                           <  2:2440675/148‑1 (MQ=255)
            ggCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGAt                                                                                                                                       <  2:151247/148‑1 (MQ=255)
               gTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGaa                                                                                                                                    <  2:171460/148‑1 (MQ=255)
                ttACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAAt                                                                                                                                   >  2:85194/1‑148 (MQ=255)
                ttACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAt                                                                                                                                   <  1:1061366/148‑1 (MQ=255)
                ttACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAt                                                                                                                                   <  2:1016499/148‑1 (MQ=255)
                ttACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAt                                                                                                                                   <  2:415082/148‑1 (MQ=255)
                 tACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGACtt                                                                                                                                  >  2:2037978/1‑148 (MQ=255)
                 tACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAtt                                                                                                                                  >  1:3407514/1‑148 (MQ=255)
                 tACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAtt                                                                                                                                  >  1:1175709/1‑148 (MQ=255)
                 tACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAtt                                                                                                                                  >  1:3282730/1‑148 (MQ=255)
                  aCTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTc                                                                                                                                 >  1:668442/1‑148 (MQ=255)
                  aCTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTc                                                                                                                                 >  1:1181036/1‑148 (MQ=255)
                   cTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCg                                                                                                                                <  1:449160/148‑1 (MQ=255)
                     aTTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCt                                                                                                                              >  1:1857102/1‑148 (MQ=255)
                     aTTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCt                                                                                                                              >  1:1856457/1‑148 (MQ=255)
                      tttCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTa                                                                                                                             <  2:582873/148‑1 (MQ=255)
                      tttCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTa                                                                                                                             >  1:2827624/1‑148 (MQ=255)
                       ttCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTAt                                                                                                                            >  1:393394/1‑148 (MQ=255)
                       ttCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTAt                                                                                                                            >  2:1628550/1‑148 (MQ=255)
                        tCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATg                                                                                                                           <  2:1549365/148‑1 (MQ=255)
                         cTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATgg                                                                                                                          >  2:3135652/1‑148 (MQ=255)
                         cTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATgg                                                                                                                          >  2:495546/1‑148 (MQ=255)
                           tGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTg                                                                                                                        <  2:1265296/148‑1 (MQ=255)
                             aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                      >  1:3368055/1‑148 (MQ=255)
                              tGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGctc                                                                                                                     >  1:2564737/1‑148 (MQ=255)
                               ggCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGctct                                                                                                                    >  1:596083/1‑148 (MQ=255)
                               ggCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGctct                                                                                                                    >  1:596097/1‑148 (MQ=255)
                                     aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatgatg                                                                                                              >  2:2494189/1‑148 (MQ=255)
                                      aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGt                                                                                                             >  2:3233256/1‑148 (MQ=255)
                                       tttCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTa                                                                                                            <  2:144650/148‑1 (MQ=255)
                                       tttCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTa                                                                                                            >  2:3093561/1‑148 (MQ=255)
                                           aaaTACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGt                                                                                                        >  1:1092000/1‑148 (MQ=255)
                                           aaaTACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGt                                                                                                        <  2:1637964/148‑1 (MQ=255)
                                             aTACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCg                                                                                                      <  2:750633/148‑1 (MQ=255)
                                               aCATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGa                                                                                                    >  1:3280596/1‑148 (MQ=255)
                                               aCATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGa                                                                                                    <  2:236485/148‑1 (MQ=255)
                                                 aTCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAt                                                                                                  <  1:658528/148‑1 (MQ=255)
                                                 aTCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAt                                                                                                  >  1:2892195/1‑148 (MQ=255)
                                                    aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTg                                                                                               >  1:158247/1‑148 (MQ=255)
                                                     aTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGa                                                                                              >  2:2312861/1‑148 (MQ=255)
                                                      tGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGaa                                                                                             <  1:2847619/148‑1 (MQ=255)
                                                       gATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAg                                                                                            <  1:2569673/148‑1 (MQ=255)
                                                         tACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTCCAGTCGGAATCTGAAGTg                                                                                          >  2:1255587/1‑148 (MQ=255)
                                                         tACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTg                                                                                          >  2:1255695/1‑148 (MQ=255)
                                                            cTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgcag                                                                                       <  1:3154042/148‑1 (MQ=255)
                                                              ggggTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgcagca                                                                                     >  2:2180787/1‑148 (MQ=255)
                                                               gggTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGcagcag                                                                                    >  2:2576499/1‑148 (MQ=255)
                                                                 gTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGat                                                                                  <  2:2945886/148‑1 (MQ=255)
                                                                 gTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGat                                                                                  <  1:3477587/148‑1 (MQ=255)
                                                                     tGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGt                                                                              >  2:2918292/1‑148 (MQ=255)
                                                                      gCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGtt                                                                             <  1:1240794/148‑1 (MQ=255)
                                                                           gggCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCa                                                                        <  1:3364124/148‑1 (MQ=255)
                                                                            ggCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCAc                                                                       <  2:307631/148‑1 (MQ=255)
                                                                              cGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTg                                                                     <  2:808590/148‑1 (MQ=255)
                                                                                 tAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGaca                                                                  <  1:3231465/148‑1 (MQ=255)
                                                                                   gagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGAcaca                                                                >  2:1772030/1‑148 (MQ=255)
                                                                                   gagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGAcaca                                                                >  1:2157887/1‑148 (MQ=255)
                                                                                       cTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATc                                                            <  2:106216/148‑1 (MQ=255)
                                                                                         tgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCtt                                                          >  1:2312220/1‑148 (MQ=255)
                                                                                         tgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCtt                                                          <  1:3135652/148‑1 (MQ=255)
                                                                                            ttgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTaa                                                       <  1:1537897/148‑1 (MQ=255)
                                                                                             tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAt                                                      <  1:822045/148‑1 (MQ=255)
                                                                                             tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAt                                                      >  1:3040146/1‑148 (MQ=255)
                                                                                                 aTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTcc                                                  <  2:1675031/148‑1 (MQ=255)
                                                                                                 aTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTcc                                                  >  2:2653970/1‑148 (MQ=255)
                                                                                                   cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCg                                                >  1:2772512/1‑148 (MQ=255)
                                                                                                      aaaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGttt                                             <  1:1918280/148‑1 (MQ=255)
                                                                                                      aaaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGttt                                             <  1:1918644/148‑1 (MQ=255)
                                                                                                         aCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAt                                          >  2:200893/1‑148 (MQ=255)
                                                                                                         aCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAt                                          >  1:1889268/1‑148 (MQ=255)
                                                                                                          cGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAATTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATc                                         >  2:3440168/1‑148 (MQ=255)
                                                                                                          cGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATc                                         <  1:2786460/148‑1 (MQ=255)
                                                                                                          cGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATc                                         <  1:3023249/148‑1 (MQ=255)
                                                                                                             ttAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTc                                      >  2:634825/1‑148 (MQ=255)
                                                                                                              tAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCa                                     >  2:2256480/1‑148 (MQ=255)
                                                                                                               aGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCAt                                    <  2:451479/148‑1 (MQ=255)
                                                                                                                    aaTTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATgg                               >  2:1399670/1‑148 (MQ=255)
                                                                                                                       ttGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCa                            >  2:1152919/1‑148 (MQ=255)
                                                                                                                       ttGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCa                            >  1:101164/1‑148 (MQ=255)
                                                                                                                        tGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCAt                           <  1:2857960/148‑1 (MQ=255)
                                                                                                                            gggCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGa                       <  1:2520364/148‑1 (MQ=255)
                                                                                                                             ggCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGac                      >  2:915676/1‑148 (MQ=255)
                                                                                                                             ggCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGac                      >  2:316703/1‑148 (MQ=255)
                                                                                                                             ggCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGac                      >  2:311200/1‑148 (MQ=255)
                                                                                                                                    aCATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATg               <  2:2845074/148‑1 (MQ=255)
                                                                                                                                    aCATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATg               <  1:3481013/148‑1 (MQ=255)
                                                                                                                                     cATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGa              >  2:3404564/1‑148 (MQ=255)
                                                                                                                                     cATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGa              <  2:1857102/148‑1 (MQ=255)
                                                                                                                                     cATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGa              <  2:1856457/148‑1 (MQ=255)
                                                                                                                                       tAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACa            >  1:2956984/1‑148 (MQ=255)
                                                                                                                                        aaaGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAt           >  1:2063583/1‑148 (MQ=255)
                                                                                                                                         aaGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAtt          <  2:3282730/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTG  >  CP009273/2736569‑2736861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGGT  >  CP009273/2736566‑2736863
                                                                                                                                                    |                                                                                                                                                     
TGCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAG                                                                                                                                                      <  SRR19973065.3463231/150‑1 (MQ=60)
        AAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCG                                                                                                                                              <  SRR19973065.2501410/150‑1 (MQ=60)
        AAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCG                                                                                                                                              <  SRR19973065.2616354/150‑1 (MQ=60)
           CGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCG                                                                                                                                           <  SRR19973065.3142436/150‑1 (MQ=60)
               GGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGA                                                                                                                                       <  SRR19973065.3277074/150‑1 (MQ=60)
               GGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGA                                                                                                                                       <  SRR19973065.1230482/150‑1 (MQ=60)
                  GTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATT                                                                                                                                    >  SRR19973065.1680092/1‑150 (MQ=60)
                  GTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATT                                                                                                                                    >  SRR19973065.3302686/1‑150 (MQ=60)
                  GTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATT                                                                                                                                    >  SRR19973065.3428332/1‑150 (MQ=60)
                  GTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATT                                                                                                                                    >  SRR19973065.1183185/1‑150 (MQ=60)
                   TTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTC                                                                                                                                   >  SRR19973065.1188500/1‑150 (MQ=60)
                   TTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTC                                                                                                                                   <  SRR19973065.1068403/150‑1 (MQ=60)
                   TTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTC                                                                                                                                   >  SRR19973065.672895/1‑150 (MQ=60)
                   TTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTC                                                                                                                                   >  SRR19973065.1188520/1‑150 (MQ=60)
                     ACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGC                                                                                                                                 >  SRR19973065.756642/1‑150 (MQ=60)
                      CTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCT                                                                                                                                >  SRR19973065.1869318/1‑150 (MQ=60)
                      CTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCT                                                                                                                                >  SRR19973065.2542710/1‑150 (MQ=60)
                      CTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCT                                                                                                                                >  SRR19973065.1869258/1‑150 (MQ=60)
                      CTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCT                                                                                                                                >  SRR19973065.1868673/1‑150 (MQ=60)
                      CTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCT                                                                                                                                <  SRR19973065.452277/150‑1 (MQ=60)
                       TATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTA                                                                                                                               >  SRR19973065.2845216/1‑150 (MQ=60)
                       TATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTA                                                                                                                               >  SRR19973065.2845074/1‑150 (MQ=60)
                        ATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTAT                                                                                                                              <  SRR19973065.3301578/150‑1 (MQ=60)
                        ATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTAT                                                                                                                              >  SRR19973065.396141/1‑150 (MQ=60)
                         TTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATG                                                                                                                             >  SRR19973065.3146365/1‑150 (MQ=60)
                              TGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCT                                                                                                                        >  SRR19973065.3388649/1‑150 (MQ=60)
                               GATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTC                                                                                                                       >  SRR19973065.2580761/1‑150 (MQ=60)
                                ATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATTCGACGGGCGATAGAGTCTTGATTGAAATAGAAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAATCGCTATGGTGCTCT                                                                                                                      >  SRR19973065.599608/1‑150 (MQ=60)
                                ATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCT                                                                                                                      >  SRR19973065.599784/1‑150 (MQ=60)
                                ATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCT                                                                                                                      >  SRR19973065.599793/1‑150 (MQ=60)
                                ATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCT                                                                                                                      >  SRR19973065.599779/1‑150 (MQ=60)
                                      ATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATG                                                                                                                <  SRR19973065.356560/150‑1 (MQ=60)
                                      ATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATG                                                                                                                >  SRR19973065.2528314/1‑150 (MQ=60)
                                      ATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATG                                                                                                                >  SRR19973065.1028249/1‑150 (MQ=60)
                                        AATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTA                                                                                                              <  SRR19973065.84925/150‑1 (MQ=60)
                                            TCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGT                                                                                                          >  SRR19973065.1099130/1‑150 (MQ=60)
                                            TCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGT                                                                                                          >  SRR19973065.1099193/1‑150 (MQ=60)
                                                ATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGA                                                                                                      >  SRR19973065.3300536/1‑150 (MQ=60)
                                                  ACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTGTTGATTGGAAGGGCCAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAT                                                                                                    >  SRR19973065.3370835/1‑150 (MQ=60)
                                                  ACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAT                                                                                                    >  SRR19973065.2909863/1‑150 (MQ=60)
                                                  ACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAT                                                                                                    >  SRR19973065.2909993/1‑150 (MQ=60)
                                                   CATCAATGTTACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGTTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATC                                                                                                   <  SRR19973065.192222/150‑1 (MQ=60)
                                                    ATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCT                                                                                                  >  SRR19973065.218408/1‑150 (MQ=60)
                                                    ATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCT                                                                                                  <  SRR19973065.665464/150‑1 (MQ=60)
                                                    ATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCT                                                                                                  <  SRR19973065.662880/150‑1 (MQ=60)
                                                    ATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCT                                                                                                  >  SRR19973065.2609674/1‑150 (MQ=60)
                                                     TCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTG                                                                                                 >  SRR19973065.159556/1‑150 (MQ=60)
                                                        ATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAG                                                                                              >  SRR19973065.1237265/1‑150 (MQ=60)
                                                         TGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGT                                                                                             >  SRR19973065.156599/1‑150 (MQ=60)
                                                         TGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGT                                                                                             <  SRR19973065.2865140/150‑1 (MQ=60)
                                                          GATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTG                                                                                            <  SRR19973065.2585715/150‑1 (MQ=60)
                                                               CTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCA                                                                                       <  SRR19973065.3173174/150‑1 (MQ=60)
                                                               CTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCA                                                                                       >  SRR19973065.2585481/1‑150 (MQ=60)
                                                                    GTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATAT                                                                                  <  SRR19973065.2297773/150‑1 (MQ=60)
                                                                    GTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATAT                                                                                  <  SRR19973065.3498747/150‑1 (MQ=60)
                                                                    GTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATAT                                                                                  <  SRR19973065.3499420/150‑1 (MQ=60)
                                                                    GTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATAT                                                                                  >  SRR19973065.2807839/1‑150 (MQ=60)
                                                                    GTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATAT                                                                                  <  SRR19973065.1298444/150‑1 (MQ=60)
                                                                     TCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATG                                                                                 <  SRR19973065.621278/150‑1 (MQ=60)
                                                                         GCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCA                                                                             <  SRR19973065.1248951/150‑1 (MQ=60)
                                                                         GCGACGGGAGATAGAGTGTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCA                                                                             <  SRR19973065.341254/150‑1 (MQ=60)
                                                                          CGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTCTGGTGCTCTATGATGTACAGTCGGAATCTGAATTGAAGCAGATATGTTCAT                                                                            >  SRR19973065.3526735/1‑150 (MQ=60)
                                                                              GGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACT                                                                        <  SRR19973065.3041144/150‑1 (MQ=60)
                                                                              GGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACT                                                                        <  SRR19973065.3384691/150‑1 (MQ=60)
                                                                              GGGCGATAGAGTCGTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACT                                                                        <  SRR19973065.3384701/150‑1 (MQ=60)
                                                                                    TAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACA                                                                  <  SRR19973065.3250885/150‑1 (MQ=60)
                                                                                    TAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACA                                                                  >  SRR19973065.2171761/1‑150 (MQ=60)
                                                                                       AGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAAT                                                               >  SRR19973065.3203319/1‑150 (MQ=60)
                                                                                          CTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTT                                                            >  SRR19973065.2326732/1‑150 (MQ=60)
                                                                                           TTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTT                                                           >  SRR19973065.34803/1‑150 (MQ=60)
                                                                                            TGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTA                                                          <  SRR19973065.3154727/150‑1 (MQ=60)
                                                                                              ATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGACTCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAT                                                        >  SRR19973065.3058754/1‑150 (MQ=60)
                                                                                              ATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAT                                                        >  SRR19973065.3058760/1‑150 (MQ=60)
                                                                                               TTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATC                                                       <  SRR19973065.1548044/150‑1 (MQ=60)
                                                                                                TGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCT                                                      <  SRR19973065.827440/150‑1 (MQ=60)
                                                                                                  AAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCC                                                    >  SRR19973065.2107732/1‑150 (MQ=60)
                                                                                                    ATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCG                                                  >  SRR19973065.2789730/1‑150 (MQ=60)
                                                                                                         AAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGA                                             <  SRR19973065.1930882/150‑1 (MQ=60)
                                                                                                         AAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGA                                             <  SRR19973065.1931229/150‑1 (MQ=60)
                                                                                                         AAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGA                                             <  SRR19973065.1930863/150‑1 (MQ=60)
                                                                                                          AAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAT                                            >  SRR19973065.2873466/1‑150 (MQ=60)
                                                                                                          AAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAT                                            >  SRR19973065.1901691/1‑150 (MQ=60)
                                                                                                             CGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTT                                         <  SRR19973065.2803739/150‑1 (MQ=60)
                                                                                                             CGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCNT                                         <  SRR19973065.3041777/150‑1 (MQ=60)
                                                                                                                  AGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAA                                    <  SRR19973065.1975805/150‑1 (MQ=60)
                                                                                                                        ATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCA                              >  SRR19973065.102094/1‑150 (MQ=60)
                                                                                                                           TGGCGGGCTGCGACATAAAGCATACCGCCTGGCCGGCGATGAATTCGCTATGGTGCTCTATTATGTACAGTCGGAAACTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCACGATTGATCTTCATAATGGAAATCA                           >  SRR19973065.40145/1‑150 (MQ=60)
                                                                                                                           TGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCA                           <  SRR19973065.1114496/150‑1 (MQ=60)
                                                                                                                           TGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCA                           <  SRR19973065.2875464/150‑1 (MQ=60)
                                                                                                                           TGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCA                           <  SRR19973065.2875516/150‑1 (MQ=60)
                                                                                                                               GGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACC                       <  SRR19973065.2536188/150‑1 (MQ=60)
                                                                                                                                  CTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACC                    <  SRR19973065.2408008/150‑1 (MQ=60)
                                                                                                                                   TGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCA                   >  SRR19973065.2881291/1‑150 (MQ=60)
                                                                                                                                    GCGACATAAAGAATACCGCATGGGCGGCGATGAATTCGCTATGGTGCTCTATGTTGTACAGTCGTAATCTTAAGAGCAGCAGATATGTTCAGCACTGACACAAAACTTTAATCTCCCGGTTGATCTTAATAAAGGACATCAGACCACCAG                  >  SRR19973065.3309590/1‑150 (MQ=60)
                                                                                                                                       ACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTGGGGTTTGATCTTCATAATGGCCATCAGACCACCATGAC               <  SRR19973065.2795700/150‑1 (MQ=60)
                                                                                                                                       ACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGAC               <  SRR19973065.3502195/150‑1 (MQ=60)
                                                                                                                                        CATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACA              >  SRR19973065.402047/1‑150 (MQ=60)
                                                                                                                                        CATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACA              >  SRR19973065.2975030/1‑150 (MQ=60)
                                                                                                                                        CATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACA              <  SRR19973065.3155258/150‑1 (MQ=60)
                                                                                                                                         ATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAT             >  SRR19973065.1894782/1‑150 (MQ=60)
                                                                                                                                         ATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAT             >  SRR19973065.2076740/1‑150 (MQ=60)
                                                                                                                                         ATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACAT             >  SRR19973065.1894774/1‑150 (MQ=60)
                                                                                                                                                ATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCAT      >  SRR19973065.1185708/1‑150 (MQ=60)
                                                                                                                                                ATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCAT      >  SRR19973065.387033/1‑150 (MQ=60)
                                                                                                                                                 TACAGCCTGGGCGGCTATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT     >  SRR19973065.1705903/1‑150 (MQ=60)
                                                                                                                                                  ACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTG    >  SRR19973065.616116/1‑150 (MQ=60)
                                                                                                                                                    AGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACCTTAAGCATTGGT  >  SRR19973065.1841279/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TGCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGGT  >  CP009273/2736566‑2736863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑