Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,736,714 C→A 100% R325S (CGC→AGC)  yfiN → putative membrane‑anchored diguanylate cyclase with an N‑terminal periplasmic domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,736,7140CA95.6% 687.0 / 19.1 227R325S (CGC→AGC) yfiNputative membrane‑anchored diguanylate cyclase with an N‑terminal periplasmic domain
Reads supporting (aligned to +/- strand):  ref base C (5/5);  new base A (125/92);  total (130/97)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 227 total aligned reads displayed.

CCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCA  >  CP009273/2736569‑2736858
                                                                                                                                                 |                                                                                                                                                
    aaaaCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTgg                                                                                                                                            >  2:2834784/1‑148 (MQ=255)
    aaaaCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTgg                                                                                                                                            <  1:232258/148‑1 (MQ=255)
       aCGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAATCATACAGCCAGggcg                                                                                                                                         >  2:923342/1‑148 (MQ=255)
          tCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGgcggcg                                                                                                                                      <  1:1569732/148‑1 (MQ=255)
              cGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGa                                                                                                                                  >  1:652056/1‑148 (MQ=255)
              cGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGa                                                                                                                                  >  1:3322064/1‑148 (MQ=255)
                ttACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAt                                                                                                                                >  1:3493861/1‑148 (MQ=255)
                  aCTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTc                                                                                                                              >  1:656971/1‑148 (MQ=255)
                   cTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCg                                                                                                                             <  2:132895/148‑1 (MQ=255)
                   cTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCg                                                                                                                             <  2:2561509/148‑1 (MQ=255)
                   cTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCg                                                                                                                             >  1:1661704/1‑148 (MQ=255)
                    tATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGc                                                                                                                            >  2:1826028/1‑148 (MQ=255)
                     aTTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCt                                                                                                                           <  1:3149455/148‑1 (MQ=255)
                     aTTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCt                                                                                                                           >  2:2232378/1‑148 (MQ=255)
                         cTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATgg                                                                                                                       <  2:1065874/148‑1 (MQ=255)
                         cTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATgg                                                                                                                       >  2:2621543/1‑148 (MQ=255)
                            gATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGc                                                                                                                    <  2:2907457/148‑1 (MQ=255)
                             aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                   <  2:2025789/148‑1 (MQ=255)
                             aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                   <  2:2027403/148‑1 (MQ=255)
                             aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                   <  2:125914/148‑1 (MQ=255)
                             aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                   <  2:1060490/148‑1 (MQ=255)
                             aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                   <  1:1284411/148‑1 (MQ=255)
                             aTGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGct                                                                                                                   >  2:2676950/1‑148 (MQ=255)
                              tGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGctc                                                                                                                  <  2:352236/148‑1 (MQ=255)
                              tGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGctc                                                                                                                  >  2:3127336/1‑148 (MQ=255)
                                gCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTa                                                                                                                <  1:546215/148‑1 (MQ=255)
                                  gATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatg                                                                                                              <  2:1535732/148‑1 (MQ=255)
                                  gATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTatg                                                                                                              >  2:1099817/1‑148 (MQ=255)
                                      aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGt                                                                                                          <  2:3355111/148‑1 (MQ=255)
                                      aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGt                                                                                                          >  2:2504928/1‑148 (MQ=255)
                                        ttCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTAc                                                                                                        >  2:262753/1‑148 (MQ=255)
                                              tACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCgg                                                                                                  >  1:1009910/1‑148 (MQ=255)
                                                 aTCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAAt                                                                                               <  1:1144126/148‑1 (MQ=255)
                                                  tCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATc                                                                                              >  1:1546887/1‑148 (MQ=255)
                                                  tCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGATGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATc                                                                                              >  2:1015186/1‑148 (MQ=255)
                                                   cAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAATCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCt                                                                                             <  2:3457110/148‑1 (MQ=255)
                                                    aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTg                                                                                            >  2:43135/1‑148 (MQ=255)
                                                       gATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAg                                                                                         >  2:3553022/1‑148 (MQ=255)
                                                        aTACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGt                                                                                        >  1:3283313/1‑148 (MQ=255)
                                                         tACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTg                                                                                       <  2:1342550/148‑1 (MQ=255)
                                                         tACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTg                                                                                       >  2:2196560/1‑148 (MQ=255)
                                                          aCCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgc                                                                                      >  2:975786/1‑148 (MQ=255)
                                                          aCCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgc                                                                                      >  1:2985023/1‑148 (MQ=255)
                                                            cTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgcag                                                                                    <  1:2626259/148‑1 (MQ=255)
                                                              ggggTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTgcagca                                                                                  >  2:2196882/1‑148 (MQ=255)
                                                               gggTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGcagcag                                                                                 <  1:2529639/148‑1 (MQ=255)
                                                                    aTGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATg                                                                            >  1:2953736/1‑148 (MQ=255)
                                                                    aTGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATg                                                                            <  1:2324311/148‑1 (MQ=255)
                                                                      gCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGtt                                                                          >  1:1876659/1‑148 (MQ=255)
                                                                         aCGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAg                                                                       <  2:652147/148‑1 (MQ=255)
                                                                            ggCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCAc                                                                    >  1:1346337/1‑148 (MQ=255)
                                                                              cGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTg                                                                  <  2:746322/148‑1 (MQ=255)
                                                                              cGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTg                                                                  <  2:98954/148‑1 (MQ=255)
                                                                                aTAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                >  2:1474604/1‑148 (MQ=255)
                                                                                aTAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                <  2:1442009/148‑1 (MQ=255)
                                                                                aTAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                >  2:3240256/1‑148 (MQ=255)
                                                                                aTAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGac                                                                <  2:3222883/148‑1 (MQ=255)
                                                                                 tAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGaca                                                               >  2:47534/1‑148 (MQ=255)
                                                                                  agagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGacac                                                              <  2:872407/148‑1 (MQ=255)
                                                                                      tCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAAt                                                          >  2:1149175/1‑148 (MQ=255)
                                                                                       cTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATc                                                         >  1:1220396/1‑148 (MQ=255)
                                                                                       cTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATc                                                         >  1:1974899/1‑148 (MQ=255)
                                                                                        ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCt                                                        <  1:3454345/148‑1 (MQ=255)
                                                                                         tgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCtt                                                       >  2:556315/1‑148 (MQ=255)
                                                                                           attgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTa                                                     >  2:227258/1‑148 (MQ=255)
                                                                                            ttgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTaa                                                    >  2:3534613/1‑148 (MQ=255)
                                                                                               aaaTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAtct                                                 <  1:1104328/148‑1 (MQ=255)
                                                                                               aaaTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAtct                                                 <  2:2998080/148‑1 (MQ=255)
                                                                                                aaTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAtctc                                                <  2:3446613/148‑1 (MQ=255)
                                                                                                 aTCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCAGCCAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTGTAAGCTcc                                               >  2:2764660/1‑148 (MQ=255)
                                                                                                  tCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTccc                                              >  2:535748/1‑148 (MQ=255)
                                                                                                  tCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTccc                                              >  2:2065348/1‑148 (MQ=255)
                                                                                                   cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCg                                             >  1:2070580/1‑148 (MQ=255)
                                                                                                      aaaaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGttt                                          >  1:3024354/1‑148 (MQ=255)
                                                                                                        aaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGa                                        >  1:2814210/1‑148 (MQ=255)
                                                                                                        aaCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGa                                        >  1:1854531/1‑148 (MQ=255)
                                                                                                         aCGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAt                                       >  1:486626/1‑148 (MQ=255)
                                                                                                          cGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATc                                      <  1:764392/148‑1 (MQ=255)
                                                                                                          cGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATc                                      <  2:3353351/148‑1 (MQ=255)
                                                                                                           ggTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCt                                     <  2:1569611/148‑1 (MQ=255)
                                                                                                            gTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCtt                                    >  1:3535229/1‑148 (MQ=255)
                                                                                                            gTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCtt                                    >  1:1693323/1‑148 (MQ=255)
                                                                                                             ttAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTc                                   <  2:441613/148‑1 (MQ=255)
                                                                                                             ttAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTc                                   >  2:1870418/1‑148 (MQ=255)
                                                                                                                   gAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATg                             <  2:180774/148‑1 (MQ=255)
                                                                                                                    aaTTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATgg                            <  1:203183/148‑1 (MQ=255)
                                                                                                                    aaTTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATgg                            <  1:2232378/148‑1 (MQ=255)
                                                                                                                      tttGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGcc                          >  1:731931/1‑148 (MQ=255)
                                                                                                                      tttGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGcc                          >  2:3322939/1‑148 (MQ=255)
                                                                                                                         ggCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATc                       <  2:2448741/148‑1 (MQ=255)
                                                                                                                              gCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGacc                  >  2:3418859/1‑148 (MQ=255)
                                                                                                                                    aCATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATg            >  2:2770224/1‑148 (MQ=255)
                                                                                                                                     cATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGa           >  2:848403/1‑148 (MQ=255)
                                                                                                                                      aTAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATATCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGAc          >  2:957129/1‑148 (MQ=255)
                                                                                                                                            cATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAg    >  1:1795662/1‑148 (MQ=255)
                                                                                                                                             aTACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGc   <  2:679751/148‑1 (MQ=255)
                                                                                                                                              tACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCa  <  1:923550/148‑1 (MQ=255)
                                                                                                                                              tACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCa  <  1:2676950/148‑1 (MQ=255)
                                                                                                                                              tACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCa  <  1:923146/148‑1 (MQ=255)
                                                                                                                                              tACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCa  <  2:1661704/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCA  >  CP009273/2736569‑2736858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGG  >  CP009273/2736569‑2736862
                                                                                                                                                 |                                                                                                                                                    
CCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAAAACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTTGCGGGCTGCGACATAAAGCATACCGCCT                                                                                                                                                  >  SRR19973067.182208/1‑150 (MQ=60)
    AAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGC                                                                                                                                              <  SRR19973067.233909/150‑1 (MQ=60)
    AAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGC                                                                                                                                              <  SRR19973067.233918/150‑1 (MQ=60)
        CGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCG                                                                                                                                          <  SRR19973067.3458652/150‑1 (MQ=60)
          TCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGAT                                                                                                                                        <  SRR19973067.1580471/150‑1 (MQ=60)
           CGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATG                                                                                                                                       >  SRR19973067.3135790/1‑150 (MQ=60)
            GGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGA                                                                                                                                      >  SRR19973067.656480/1‑150 (MQ=60)
            GGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAAAGGTTAGCTGAATTTGGCGGGCTGCGACATAAATCATACAGCCTGGGCGGCGATGA                                                                                                                                      >  SRR19973067.3343078/1‑150 (MQ=60)
            GGCGTTACTATTTCTTGATGGCGATAATCTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGA                                                                                                                                      >  SRR19973067.656935/1‑150 (MQ=60)
             GCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAA                                                                                                                                     <  SRR19973067.3043024/150‑1 (MQ=60)
              CGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAT                                                                                                                                    >  SRR19973067.3516028/1‑150 (MQ=60)
              CGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAAT                                                                                                                                    >  SRR19973067.3516021/1‑150 (MQ=60)
                TTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTC                                                                                                                                  >  SRR19973067.661436/1‑150 (MQ=60)
                 TACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCG                                                                                                                                 >  SRR19973067.1673167/1‑150 (MQ=60)
                     ATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTAT                                                                                                                             <  SRR19973067.3169433/150‑1 (MQ=60)
                       TTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGTGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGG                                                                                                                           >  SRR19973067.1266759/1‑150 (MQ=60)
                       TTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGTGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGG                                                                                                                           >  SRR19973067.1268109/1‑150 (MQ=60)
                             ATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCT                                                                                                                     >  SRR19973067.2297956/1‑150 (MQ=60)
                             ATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCT                                                                                                                     <  SRR19973067.1293163/150‑1 (MQ=60)
                                GCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATG                                                                                                                  <  SRR19973067.549827/150‑1 (MQ=60)
                                GCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATG                                                                                                                  <  SRR19973067.549877/150‑1 (MQ=60)
                                   ATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATG                                                                                                               >  SRR19973067.536298/1‑150 (MQ=60)
                                    TAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGT                                                                                                              >  SRR19973067.2892052/1‑150 (MQ=60)
                                            AATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGG                                                                                                      >  SRR19973067.1016847/1‑150 (MQ=60)
                                            AATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGG                                                                                                      >  SRR19973067.1017764/1‑150 (MQ=60)
                                             ATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGA                                                                                                     >  SRR19973067.1735971/1‑150 (MQ=60)
                                             ATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGA                                                                                                     >  SRR19973067.2973092/1‑150 (MQ=60)
                                              TACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAA                                                                                                    >  SRR19973067.1674321/1‑150 (MQ=60)
                                                CATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATC                                                                                                  >  SRR19973067.1557405/1‑150 (MQ=60)
                                                 ATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCT                                                                                                 <  SRR19973067.1151799/150‑1 (MQ=60)
                                                     ATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAG                                                                                             >  SRR19973067.1974855/1‑150 (MQ=60)
                                                      TGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGT                                                                                            >  SRR19973067.3304656/1‑150 (MQ=60)
                                                      TGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGT                                                                                            >  SRR19973067.3303911/1‑150 (MQ=60)
                                                        ATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGC                                                                                          >  SRR19973067.3003894/1‑150 (MQ=60)
                                                            CTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCA                                                                                      <  SRR19973067.2643229/150‑1 (MQ=60)
                                                            CTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCA                                                                                      <  SRR19973067.2643280/150‑1 (MQ=60)
                                                               GGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGAT                                                                                   <  SRR19973067.2546186/150‑1 (MQ=60)
                                                               GGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGAT                                                                                   <  SRR19973067.2546215/150‑1 (MQ=60)
                                                                  TCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATG                                                                                >  SRR19973067.2972415/1‑150 (MQ=60)
                                                                    ATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTT                                                                              >  SRR19973067.1889464/1‑150 (MQ=60)
                                                                    ATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTT                                                                              <  SRR19973067.2339581/150‑1 (MQ=60)
                                                                    ATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTT                                                                              >  SRR19973067.1575883/1‑150 (MQ=60)
                                                                     TGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTC                                                                             <  SRR19973067.1877365/150‑1 (MQ=60)
                                                                     TGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTC                                                                             <  SRR19973067.162052/150‑1 (MQ=60)
                                                                     TGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTC                                                                             <  SRR19973067.1877337/150‑1 (MQ=60)
                                                                          CGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCAC                                                                        >  SRR19973067.1355541/1‑150 (MQ=60)
                                                                          CGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCAC                                                                        >  SRR19973067.1355546/1‑150 (MQ=60)
                                                                           GGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACT                                                                       >  SRR19973067.746561/1‑150 (MQ=60)
                                                                                    AGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAAT                                                              >  SRR19973067.20740/1‑150 (MQ=60)
                                                                                     GTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATC                                                             >  SRR19973067.2183907/1‑150 (MQ=60)
                                                                                     GTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATC                                                             >  SRR19973067.1228512/1‑150 (MQ=60)
                                                                                     GTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATC                                                             >  SRR19973067.1988282/1‑150 (MQ=60)
                                                                                     GTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATC                                                             >  SRR19973067.928959/1‑150 (MQ=60)
                                                                                       CTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTT                                                           <  SRR19973067.988030/150‑1 (MQ=60)
                                                                                        TTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTT                                                          <  SRR19973067.3476319/150‑1 (MQ=60)
                                                                                           ATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAAT                                                       <  SRR19973067.1544995/150‑1 (MQ=60)
                                                                                             TGAAATCTCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACCGTCGGAATCTGAAGAGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCT                                                     >  SRR19973067.3251119/1‑150 (MQ=60)
                                                                                             TGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCT                                                     <  SRR19973067.458457/150‑1 (MQ=60)
                                                                                               AAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCC                                                   <  SRR19973067.1111832/150‑1 (MQ=60)
                                                                                               AAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCC                                                   <  SRR19973067.1112702/150‑1 (MQ=60)
                                                                                                 ATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCG                                                 >  SRR19973067.2084337/1‑150 (MQ=60)
                                                                                                 ATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCG                                                 >  SRR19973067.2083659/1‑150 (MQ=60)
                                                                                                 ATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCG                                                 <  SRR19973067.3502738/150‑1 (MQ=60)
                                                                                                    GCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTT                                              >  SRR19973067.3043609/1‑150 (MQ=60)
                                                                                                      AAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGA                                            >  SRR19973067.1867170/1‑150 (MQ=60)
                                                                                                      AAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGA                                            >  SRR19973067.2832348/1‑150 (MQ=60)
                                                                                                      AAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGA                                            >  SRR19973067.874291/1‑150 (MQ=60)
                                                                                                      AAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGA                                            >  SRR19973067.2832339/1‑150 (MQ=60)
                                                                                                       AAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAT                                           >  SRR19973067.489976/1‑150 (MQ=60)
                                                                                                       AAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGAT                                           <  SRR19973067.63614/150‑1 (MQ=60)
                                                                                                          CGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTT                                        >  SRR19973067.3557496/1‑150 (MQ=60)
                                                                                                          CGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTT                                        >  SRR19973067.3557572/1‑150 (MQ=60)
                                                                                                          CGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTT                                        <  SRR19973067.769702/150‑1 (MQ=60)
                                                                                                          CGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTT                                        >  SRR19973067.1704979/1‑150 (MQ=60)
                                                                                                                GCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAAT                                  <  SRR19973067.2011484/150‑1 (MQ=60)
                                                                                                                GCTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAAT                                  <  SRR19973067.2538191/150‑1 (MQ=60)
                                                                                                                 CTGAATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATG                                 >  SRR19973067.313918/1‑150 (MQ=60)
                                                                                                                   GAATTTGGCGGGCTGCGACATAAAGCATACAGACTGGGCGGCGATGAATTCGATATGGTGCTCTATGATTTACAGTCGGAATCTGAAGTGCAGCAGATAAGTTCAGCACTGAAACAAATCATTAATCTCCCGAATTATCTTCATAATGGA                               >  SRR19973067.2719295/1‑150 (MQ=60)
                                                                                                                    AATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCC                              >  SRR19973067.737014/1‑150 (MQ=60)
                                                                                                                    AATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCC                              <  SRR19973067.204727/150‑1 (MQ=60)
                                                                                                                    AATTTGGCGGGCTGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCC                              <  SRR19973067.2247228/150‑1 (MQ=60)
                                                                                                                                TGCGACATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCA                  >  SRR19973067.2661073/1‑150 (MQ=60)
                                                                                                                                     CATAAAGCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACA             >  SRR19973067.1202180/1‑150 (MQ=60)
                                                                                                                                        AAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTA          >  SRR19973067.1590317/1‑150 (MQ=60)
                                                                                                                                          AGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAG        >  SRR19973067.1807741/1‑150 (MQ=60)
                                                                                                                                           GCATACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGC       >  SRR19973067.667813/1‑150 (MQ=60)
                                                                                                                                              TACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT    <  SRR19973067.2694116/150‑1 (MQ=60)
                                                                                                                                              TACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT    <  SRR19973067.929353/150‑1 (MQ=60)
                                                                                                                                              TACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT    <  SRR19973067.929552/150‑1 (MQ=60)
                                                                                                                                              TACAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATT    <  SRR19973067.929763/150‑1 (MQ=60)
                                                                                                                                                CAGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGG  <  SRR19973067.43728/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
CCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAGTCGGAATCTGAAGTGCAGCAGATATGTTCAGCACTGACACAAATCTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACCACCATGACATTAAGCATTGG  >  CP009273/2736569‑2736862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑