Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,084,798 C→A 100% L102L (CTC→CTA)  rsmE → 16S rRNA m(3)U1498 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,084,7980CA97.0% 699.4 / 8.9 230L102L (CTC→CTA) rsmE16S rRNA m(3)U1498 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (110/113);  total (113/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 230 total aligned reads displayed.

TTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATC  >  CP009273/3084653‑3084942
                                                                                                                                                 |                                                                                                                                                
ttACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTTGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAgg                                                                                                                                                <  2:81670/148‑1 (MQ=255)
ttACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAgg                                                                                                                                                <  2:3115991/148‑1 (MQ=255)
ttACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAgg                                                                                                                                                <  1:391856/148‑1 (MQ=255)
  aCCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGgtg                                                                                                                                              >  2:1231643/1‑148 (MQ=255)
     aGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTaa                                                                                                                                           >  1:179883/1‑148 (MQ=255)
          cAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTc                                                                                                                                      <  1:1311675/148‑1 (MQ=255)
          cAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTc                                                                                                                                      >  1:2226337/1‑148 (MQ=255)
           accAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCa                                                                                                                                     >  2:712063/3‑148 (MQ=255)
           aGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCa                                                                                                                                     <  2:799930/148‑1 (MQ=255)
            gCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAt                                                                                                                                    >  1:1912924/1‑148 (MQ=255)
              aaaaaaaGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTa                                                                                                                                  <  1:435103/148‑1 (MQ=255)
              aaaaaaaGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTa                                                                                                                                  <  1:2230248/148‑1 (MQ=255)
                aaaaaGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACg                                                                                                                                >  2:649125/1‑148 (MQ=255)
                    aGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCAc                                                                                                                            <  2:2932832/148‑1 (MQ=255)
                      cGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACtt                                                                                                                          >  2:240622/1‑148 (MQ=255)
                         ggAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACttttt                                                                                                                       >  2:427595/1‑148 (MQ=255)
                          gAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACtttttt                                                                                                                      >  2:3445151/1‑148 (MQ=255)
                          gAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACtttttt                                                                                                                      <  1:3526923/148‑1 (MQ=255)
                          gAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACtttttt                                                                                                                      >  1:3042343/1‑148 (MQ=255)
                          gAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACtttttt                                                                                                                      >  1:1548010/1‑148 (MQ=255)
                             gTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTg                                                                                                                   <  1:912364/148‑1 (MQ=255)
                               gAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAg                                                                                                                 >  2:1916421/1‑148 (MQ=255)
                                 aGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAgcg                                                                                                               <  2:1835666/148‑1 (MQ=255)
                                  ggTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAgcgc                                                                                                              >  1:3525151/1‑148 (MQ=255)
                                  ggTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAgcgc                                                                                                              >  1:2729957/1‑148 (MQ=255)
                                    tGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTg                                                                                                            <  1:2920171/148‑1 (MQ=255)
                                      cTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTgcg                                                                                                          >  1:3278232/1‑148 (MQ=255)
                                        ggAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTgcggc                                                                                                        >  2:1197263/1‑148 (MQ=255)
                                         gAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTgcggcg                                                                                                       >  1:3334537/1‑148 (MQ=255)
                                         gAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTgcggcg                                                                                                       <  1:2558614/148‑1 (MQ=255)
                                         gAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTgcggcg                                                                                                       <  1:2209606/148‑1 (MQ=255)
                                               cAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAAc                                                                                                 >  1:2380362/1‑148 (MQ=255)
                                                aGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACt                                                                                                <  2:36931/148‑1 (MQ=255)
                                                  aTCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTgg                                                                                              <  2:2764905/148‑1 (MQ=255)
                                                   tCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGa                                                                                             <  2:2525264/148‑1 (MQ=255)
                                                   tCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGa                                                                                             <  2:2619168/148‑1 (MQ=255)
                                                     gacgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATa                                                                                           <  2:2387501/148‑1 (MQ=255)
                                                       cgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGt                                                                                         <  1:2341538/148‑1 (MQ=255)
                                                       cgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGt                                                                                         <  1:3463387/148‑1 (MQ=255)
                                                       cgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGt                                                                                         <  1:1698102/148‑1 (MQ=255)
                                                       cgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGt                                                                                         <  2:3051826/148‑1 (MQ=255)
                                                        gaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTg                                                                                        <  1:2421777/148‑1 (MQ=255)
                                                        gaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTg                                                                                        <  1:2421130/148‑1 (MQ=255)
                                                         aTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGa                                                                                       <  1:689148/148‑1 (MQ=255)
                                                                 tctcCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGa                                                                               >  1:1635891/1‑148 (MQ=255)
                                                                 tctcCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGa                                                                               >  2:1808222/1‑148 (MQ=255)
                                                                 tctcCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGa                                                                               >  2:3110465/1‑148 (MQ=255)
                                                                  ctcCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGaa                                                                              <  2:1640964/148‑1 (MQ=255)
                                                                       cTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACaaga                                                                         <  2:2348645/148‑1 (MQ=255)
                                                                         gCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACaagaag                                                                       <  2:3342089/148‑1 (MQ=255)
                                                                             atTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTc                                                                   >  1:3412724/1‑148 (MQ=255)
                                                                             atTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTc                                                                   <  1:1395066/148‑1 (MQ=255)
                                                                               tCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTcag                                                                 >  1:2598709/1‑148 (MQ=255)
                                                                                   cTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGt                                                             <  2:1029244/148‑1 (MQ=255)
                                                                                     cGGTCAGTTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAAAAAGAAGCTTCAGCAGTgg                                                           <  2:403266/148‑1 (MQ=255)
                                                                                     cGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTgg                                                           >  1:556327/1‑148 (MQ=255)
                                                                                     cGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTgg                                                           <  2:2197185/148‑1 (MQ=255)
                                                                                     cGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTgg                                                           >  1:1855237/1‑148 (MQ=255)
                                                                                        tCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCag                                                        >  2:1410690/1‑148 (MQ=255)
                                                                                          aGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCagaa                                                      <  1:2934795/148‑1 (MQ=255)
                                                                                           ggTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCagaag                                                     >  1:1350458/1‑148 (MQ=255)
                                                                                             tGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGAt                                                   >  2:2179507/1‑148 (MQ=255)
                                                                                               aTGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTg                                                 >  2:423052/1‑148 (MQ=255)
                                                                                                tGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGc                                                >  2:875170/1‑148 (MQ=255)
                                                                                                 gTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCa                                               <  2:3517227/148‑1 (MQ=255)
                                                                                                   cgcgTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAAt                                             >  2:352582/1‑148 (MQ=255)
                                                                                                   cgcgTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAAt                                             <  1:1418879/148‑1 (MQ=255)
                                                                                                         gtgAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATtgctgc                                       <  2:1593389/148‑1 (MQ=255)
                                                                                                            aaaaaaTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCtg                                    >  2:1355262/1‑148 (MQ=255)
                                                                                                              aaaaTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCtgtg                                  >  1:3108472/1‑148 (MQ=255)
                                                                                                              aaaaTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCtgtg                                  <  1:1090286/148‑1 (MQ=255)
                                                                                                                aaTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGACGATTGCAAGTGCTGCCTGTGAg                                >  1:2739890/1‑148 (MQ=255)
                                                                                                                 aTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGc                               <  1:1937203/148‑1 (MQ=255)
                                                                                                                 aTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGc                               >  2:2881915/1‑148 (MQ=255)
                                                                                                                  tGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCa                              >  1:1154944/1‑148 (MQ=255)
                                                                                                                  tGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCa                              <  2:3195508/148‑1 (MQ=255)
                                                                                                                  tGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCa                              <  1:2808146/148‑1 (MQ=255)
                                                                                                                      aTTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAgtgt                          <  2:2226337/148‑1 (MQ=255)
                                                                                                                       tttACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCACTTGCTGCCTGTGAGCAGtgtg                         >  2:907378/1‑148 (MQ=255)
                                                                                                                       tttACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGtgtg                         >  1:2023887/1‑148 (MQ=255)
                                                                                                                       ttgaCTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGtgtg                         <  1:2626488/145‑1 (MQ=255)
                                                                                                                         tACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGt                       <  2:2792993/148‑1 (MQ=255)
                                                                                                                           cTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCg                     >  1:3443547/1‑148 (MQ=255)
                                                                                                                            tATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGt                    >  2:588543/1‑148 (MQ=255)
                                                                                                                               ccAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAAc                 >  1:705769/1‑148 (MQ=255)
                                                                                                                                 aGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCg               <  1:844238/148‑1 (MQ=255)
                                                                                                                                  gAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCgg              >  2:158886/1‑148 (MQ=255)
                                                                                                                                     atcgatcgAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTg           <  1:531339/148‑1 (MQ=255)
                                                                                                                                      tcgatcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGc          >  1:1475100/1‑148 (MQ=255)
                                                                                                                                      tcgatcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGc          <  2:55386/148‑1 (MQ=255)
                                                                                                                                       cgatcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGcc         <  2:1532084/148‑1 (MQ=255)
                                                                                                                                         atcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCgg       >  2:502239/1‑148 (MQ=255)
                                                                                                                                          tcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGa      >  2:1692638/1‑148 (MQ=255)
                                                                                                                                          tcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGa      >  1:2514568/1‑148 (MQ=255)
                                                                                                                                          tcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGa      >  2:1089532/1‑148 (MQ=255)
                                                                                                                                           cgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGaa     <  1:3360477/148‑1 (MQ=255)
                                                                                                                                           cgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGaa     <  1:3445151/148‑1 (MQ=255)
                                                                                                                                           cgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGaa     <  1:2175506/148‑1 (MQ=255)
                                                                                                                                           cgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGaa     <  2:130177/148‑1 (MQ=255)
                                                                                                                                             aaCTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAAt   <  1:757579/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATC  >  CP009273/3084653‑3084942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCG  >  CP009273/3084650‑3084944
                                                                                                                                                    |                                                                                                                                                  
AAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAG                                                                                                                                                   <  SRR19973066.2793052/150‑1 (MQ=60)
   TTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTG                                                                                                                                                <  SRR19973066.394945/150‑1 (MQ=60)
     ACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTA                                                                                                                                              >  SRR19973066.553303/1‑150 (MQ=60)
      CCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAA                                                                                                                                             >  SRR19973066.181459/1‑150 (MQ=60)
          CGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCT                                                                                                                                         <  SRR19973066.511164/150‑1 (MQ=60)
           GCCAGCAAAAAAAGCGTGGAATTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCAGCATATACACCTCGGTCAGGAGAATTCGCGTGGTGAAAAAATGGAATTTACTATCAACAAATCGATCGAACTAGGTGTAAGCCTC                                                                                                                                        >  SRR19973066.563724/1‑150 (MQ=60)
           GCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTC                                                                                                                                        >  SRR19973066.2241724/1‑150 (MQ=60)
             CAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAT                                                                                                                                      <  SRR19973066.3107357/150‑1 (MQ=60)
             CAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAT                                                                                                                                      >  SRR19973066.1926548/1‑150 (MQ=60)
             CAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAT                                                                                                                                      <  SRR19973066.1321194/150‑1 (MQ=60)
             CAGCAAAAAAAGCGTGGAAGTGAAGGTGCCGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAT                                                                                                                                      <  SRR19973066.1321257/150‑1 (MQ=60)
              AGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATT                                                                                                                                     >  SRR19973066.835966/1‑150 (MQ=60)
               GCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTA                                                                                                                                    <  SRR19973066.2382943/150‑1 (MQ=60)
               GCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTA                                                                                                                                    <  SRR19973066.599084/150‑1 (MQ=60)
                 AAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACG                                                                                                                                  <  SRR19973066.438472/150‑1 (MQ=60)
                 AAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACG                                                                                                                                  <  SRR19973066.165585/150‑1 (MQ=60)
                 AAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACG                                                                                                                                  <  SRR19973066.2245650/150‑1 (MQ=60)
                   AAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCC                                                                                                                                >  SRR19973066.1510154/1‑150 (MQ=60)
                      AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                             <  SRR19973066.3469954/150‑1 (MQ=60)
                        GCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTT                                                                                                                           <  SRR19973066.1563075/150‑1 (MQ=60)
                         CGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTT                                                                                                                          >  SRR19973066.2585216/1‑150 (MQ=60)
                           TGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTT                                                                                                                        >  SRR19973066.1559097/1‑150 (MQ=60)
                           TGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTT                                                                                                                        >  SRR19973066.3062673/1‑150 (MQ=60)
                            GGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTC                                                                                                                       >  SRR19973066.1859097/1‑150 (MQ=60)
                             GAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCT                                                                                                                      <  SRR19973066.3550390/150‑1 (MQ=60)
                             GAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCT                                                                                                                      <  SRR19973066.3240764/150‑1 (MQ=60)
                             GAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCT                                                                                                                      <  SRR19973066.2532135/150‑1 (MQ=60)
                              AAGTGAAGGTGCTGGAAGGCCAGATCGACGATCTCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTG                                                                                                                     >  SRR19973066.3328427/1‑150 (MQ=60)
                              AAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTG                                                                                                                     <  SRR19973066.2245247/150‑1 (MQ=60)
                                GTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAG                                                                                                                   <  SRR19973066.405730/150‑1 (MQ=60)
                                GTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAG                                                                                                                   <  SRR19973066.918851/150‑1 (MQ=60)
                                  GAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCG                                                                                                                 >  SRR19973066.1797850/1‑150 (MQ=60)
                                   AAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGC                                                                                                                >  SRR19973066.2748315/1‑150 (MQ=60)
                                   AAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGC                                                                                                                >  SRR19973066.3548613/1‑150 (MQ=60)
                                       TGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCG                                                                                                            <  SRR19973066.2939638/150‑1 (MQ=60)
                                       TGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCG                                                                                                            >  SRR19973066.3299760/1‑150 (MQ=60)
                                          TGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCG                                                                                                         >  SRR19973066.3356805/1‑150 (MQ=60)
                                          TGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCG                                                                                                         >  SRR19973066.3356717/1‑150 (MQ=60)
                                            GAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTT                                                                                                       >  SRR19973066.403856/1‑150 (MQ=60)
                                            GAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTT                                                                                                       <  SRR19973066.2224911/150‑1 (MQ=60)
                                            GAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTT                                                                                                       <  SRR19973066.2575982/150‑1 (MQ=60)
                                            GAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTT                                                                                                       >  SRR19973066.3549945/1‑150 (MQ=60)
                                                GCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAAC                                                                                                   >  SRR19973066.2396418/1‑150 (MQ=60)
                                                    GATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGA                                                                                               >  SRR19973066.131456/1‑150 (MQ=60)
                                                      TCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATA                                                                                             <  SRR19973066.1780055/150‑1 (MQ=60)
                                                          CGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGA                                                                                         <  SRR19973066.2357612/150‑1 (MQ=60)
                                                          CGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGA                                                                                         <  SRR19973066.3486540/150‑1 (MQ=60)
                                                          CGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGA                                                                                         <  SRR19973066.1710443/150‑1 (MQ=60)
                                                          CGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGA                                                                                         <  SRR19973066.2357480/150‑1 (MQ=60)
                                                           GATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGNA                                                                                        <  SRR19973066.2437563/150‑1 (MQ=60)
                                                           GATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAA                                                                                        <  SRR19973066.2438210/150‑1 (MQ=60)
                                                           GATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAA                                                                                        <  SRR19973066.2438214/150‑1 (MQ=60)
                                                            ATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAAC                                                                                       <  SRR19973066.2869483/150‑1 (MQ=60)
                                                            ATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAAC                                                                                       <  SRR19973066.694220/150‑1 (MQ=60)
                                                                CGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCT                                                                                   >  SRR19973066.2952278/1‑150 (MQ=60)
                                                                  AATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGA                                                                                 >  SRR19973066.1647785/1‑150 (MQ=60)
                                                                       CCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAG                                                                            <  SRR19973066.835218/150‑1 (MQ=60)
                                                                        CGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGA                                                                           <  SRR19973066.2676001/150‑1 (MQ=60)
                                                                              ATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTC                                                                     <  SRR19973066.1506961/150‑1 (MQ=60)
                                                                              ATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTC                                                                     >  SRR19973066.3435547/1‑150 (MQ=60)
                                                                              ATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTC                                                                     <  SRR19973066.3530682/150‑1 (MQ=60)
                                                                              ATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTC                                                                     >  SRR19973066.3435541/1‑150 (MQ=60)
                                                                                ATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAG                                                                   <  SRR19973066.1405233/150‑1 (MQ=60)
                                                                                ATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAG                                                                   >  SRR19973066.2616298/1‑150 (MQ=60)
                                                                                ATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAG                                                                   <  SRR19973066.1405120/150‑1 (MQ=60)
                                                                                    ACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGT                                                               >  SRR19973066.1365537/1‑150 (MQ=60)
                                                                                      CTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGG                                                             >  SRR19973066.1868520/1‑150 (MQ=60)
                                                                                      CTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGG                                                             >  SRR19973066.1868511/1‑150 (MQ=60)
                                                                                      CTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGG                                                             >  SRR19973066.560315/1‑150 (MQ=60)
                                                                                         GGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAG                                                          >  SRR19973066.2237014/1‑150 (MQ=60)
                                                                                         GGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAG                                                          >  SRR19973066.2234264/1‑150 (MQ=60)
                                                                                            CAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAG                                                       >  SRR19973066.1360453/1‑150 (MQ=60)
                                                                                            CAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAG                                                       >  SRR19973066.1360245/1‑150 (MQ=60)
                                                                                             AGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGA                                                      >  SRR19973066.348858/1‑150 (MQ=60)
                                                                                             AGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGA                                                      <  SRR19973066.2954317/150‑1 (MQ=60)
                                                                                              GGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGAT                                                     <  SRR19973066.2673911/150‑1 (MQ=60)
                                                                                              GGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGAT                                                     <  SRR19973066.2568805/150‑1 (MQ=60)
                                                                                               GTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATT                                                    <  SRR19973066.1151955/150‑1 (MQ=60)
                                                                                                      CGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTG                                             <  SRR19973066.1429041/150‑1 (MQ=60)
                                                                                                      CGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTG                                             >  SRR19973066.2075970/1‑150 (MQ=60)
                                                                                                      CGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTG                                             <  SRR19973066.3583297/150‑1 (MQ=60)
                                                                                                      CGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTG                                             >  SRR19973066.262492/1‑150 (MQ=60)
                                                                                                             TGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTG                                      <  SRR19973066.3424304/150‑1 (MQ=60)
                                                                                                               ANAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTG                                    >  SRR19973066.3129235/1‑150 (MQ=60)
                                                                                                                AAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGA                                   >  SRR19973066.735530/1‑150 (MQ=60)
                                                                                                                 AAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGACGATTGCAAGTGCTGCCTGTGAG                                  >  SRR19973066.2758319/1‑150 (MQ=60)
                                                                                                                 AAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAG                                  <  SRR19973066.1098232/150‑1 (MQ=60)
                                                                                                                 AAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAG                                  >  SRR19973066.707073/1‑150 (MQ=60)
                                                                                                                 AAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAG                                  >  SRR19973066.2507638/1‑150 (MQ=60)
                                                                                                                 AAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAG                                  >  SRR19973066.2758126/1‑150 (MQ=60)
                                                                                                                   AATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCA                                >  SRR19973066.1163159/1‑150 (MQ=60)
                                                                                                                    ATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAG                               <  SRR19973066.1950911/150‑1 (MQ=60)
                                                                                                                    ATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAG                               <  SRR19973066.1950949/150‑1 (MQ=60)
                                                                                                                    ATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAG                               <  SRR19973066.1950950/150‑1 (MQ=60)
                                                                                                                     TGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGT                              <  SRR19973066.2827148/150‑1 (MQ=60)
                                                                                                                     TGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGT                              <  SRR19973066.2827040/150‑1 (MQ=60)
                                                                                                                      GGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTG                             <  SRR19973066.1475584/150‑1 (MQ=60)
                                                                                                                      GGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTG                             <  SRR19973066.242506/150‑1 (MQ=60)
                                                                                                                        AATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTG                           >  SRR19973066.2038032/1‑150 (MQ=60)
                                                                                                                        AATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTG                           >  SRR19973066.2038037/1‑150 (MQ=60)
                                                                                                                          TTGACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGT                         <  SRR19973066.2644193/150‑1 (MQ=60)
                                                                                                                            TACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCG                       >  SRR19973066.3466586/1‑150 (MQ=60)
                                                                                                                            TACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCG                       >  SRR19973066.3467446/1‑150 (MQ=60)
                                                                                                                                ATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAAC                   >  SRR19973066.710987/1‑150 (MQ=60)
                                                                                                                                    AGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGG               >  SRR19973066.2788462/1‑150 (MQ=60)
                                                                                                                                    AGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGG               <  SRR19973066.341444/150‑1 (MQ=60)
                                                                                                                                    AGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGG               <  SRR19973066.850367/150‑1 (MQ=60)
                                                                                                                                       AATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGC            >  SRR19973066.2299154/1‑150 (MQ=60)
                                                                                                                                       AATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGC            >  SRR19973066.1485488/1‑150 (MQ=60)
                                                                                                                                        ATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCC           <  SRR19973066.535221/150‑1 (MQ=60)
                                                                                                                                        ATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCC           <  SRR19973066.3045359/150‑1 (MQ=60)
                                                                                                                                           GATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGA        >  SRR19973066.900983/1‑150 (MQ=60)
                                                                                                                                           GATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGA        >  SRR19973066.2531677/1‑150 (MQ=60)
                                                                                                                                              CGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAAT     <  SRR19973066.2190615/150‑1 (MQ=60)
                                                                                                                                              CGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAAT     <  SRR19973066.3166176/150‑1 (MQ=60)
                                                                                                                                              CGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAAT     <  SRR19973066.3468194/150‑1 (MQ=60)
                                                                                                                                              CGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAAT     <  SRR19973066.2190730/150‑1 (MQ=60)
                                                                                                                                              CGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAAT     <  SRR19973066.3382884/150‑1 (MQ=60)
                                                                                                                                                AACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACANGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCC   >  SRR19973066.1821507/1‑150 (MQ=60)
                                                                                                                                                AACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCC   <  SRR19973066.763191/150‑1 (MQ=60)
                                                                                                                                                 ACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCG  >  SRR19973066.811806/1‑150 (MQ=60)
                                                                                                                                                 ACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCG  >  SRR19973066.2562768/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
AAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCG  >  CP009273/3084650‑3084944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑