Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,084,798 C→A 100% L102L (CTC→CTA)  rsmE → 16S rRNA m(3)U1498 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,084,7980CA98.3% 717.5 / 3.0 230L102L (CTC→CTA) rsmE16S rRNA m(3)U1498 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (116/110);  total (119/111)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

AATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCC  >  CP009273/3084651‑3084943
                                                                                                                                                   |                                                                                                                                                 
  ttACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGgt                                                                                                                                                >  1:1673263/1‑149 (MQ=255)
       aGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAg                                                                                                                                           >  1:415131/1‑149 (MQ=255)
        gcgcCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGc                                                                                                                                          <  2:3087932/149‑1 (MQ=255)
        gcgcCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGc                                                                                                                                          >  1:2205573/1‑149 (MQ=255)
          gcCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCt                                                                                                                                        <  2:1631292/149‑1 (MQ=255)
            cAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCa                                                                                                                                      <  1:3532932/149‑1 (MQ=255)
             aGCAAACAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAt                                                                                                                                     <  2:2805740/149‑1 (MQ=255)
             aGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAt                                                                                                                                     <  1:3017056/149‑1 (MQ=255)
             aGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAt                                                                                                                                     <  2:1742150/149‑1 (MQ=255)
              gCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAtt                                                                                                                                    <  1:1453193/149‑1 (MQ=255)
                aaaaaaaGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTAc                                                                                                                                  <  2:2238343/149‑1 (MQ=255)
                    aaaGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCa                                                                                                                              >  2:851662/1‑149 (MQ=255)
                    aaaGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGACTCATTACGCCa                                                                                                                              >  2:2911998/1‑149 (MQ=255)
                     aaGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCAc                                                                                                                             <  1:1915181/149‑1 (MQ=255)
                     aaGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCAc                                                                                                                             <  1:474934/149‑1 (MQ=255)
                      aGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACt                                                                                                                            >  1:3866246/1‑149 (MQ=255)
                      aGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACt                                                                                                                            >  1:2326931/1‑149 (MQ=255)
                       gCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACtt                                                                                                                           <  2:3873237/149‑1 (MQ=255)
                       gCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACtt                                                                                                                           <  2:3602293/149‑1 (MQ=255)
                        cGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACttt                                                                                                                          >  2:3575554/1‑149 (MQ=255)
                               gTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGa                                                                                                                   <  1:2408718/149‑1 (MQ=255)
                               gTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGa                                                                                                                   <  1:549622/149‑1 (MQ=255)
                                   aGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAgcgc                                                                                                               <  2:2588519/149‑1 (MQ=255)
                                   aGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAgcgc                                                                                                               <  1:730724/149‑1 (MQ=255)
                                   aGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAgcgc                                                                                                               >  2:1770754/1‑149 (MQ=255)
                                            aaGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGtt                                                                                                      <  2:3229082/149‑1 (MQ=255)
                                             aGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTa                                                                                                     >  2:3697006/1‑149 (MQ=255)
                                              ggCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTaa                                                                                                    >  1:311685/1‑149 (MQ=255)
                                              ggCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTaa                                                                                                    >  1:3428514/1‑149 (MQ=255)
                                               gCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTaaa                                                                                                   >  2:14319/1‑149 (MQ=255)
                                                 cAGATAGACGATCGCGAATCTACGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGACATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACt                                                                                                 >  2:715134/1‑149 (MQ=255)
                                                      cgacgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATa                                                                                            <  2:810793/149‑1 (MQ=255)
                                                       gacgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAg                                                                                           <  1:3795160/149‑1 (MQ=255)
                                                        acgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGt                                                                                          >  1:3039004/1‑149 (MQ=255)
                                                        acgaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGt                                                                                          >  1:2450729/1‑149 (MQ=255)
                                                          gaTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGa                                                                                        <  2:3919556/149‑1 (MQ=255)
                                                           aTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTTATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGaa                                                                                       <  2:1148745/149‑1 (MQ=255)
                                                           aTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGaa                                                                                       <  2:3203322/149‑1 (MQ=255)
                                                           aTCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGaa                                                                                       <  1:2619838/149‑1 (MQ=255)
                                                                gAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCt                                                                                  >  1:1501231/1‑149 (MQ=255)
                                                                 aaTCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTg                                                                                 <  1:723567/149‑1 (MQ=255)
                                                                 aaTCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTg                                                                                 >  2:2860941/1‑149 (MQ=255)
                                                                  aTCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGa                                                                                >  2:2835768/1‑149 (MQ=255)
                                                                   tctcCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGaa                                                                               <  1:2178955/149‑1 (MQ=255)
                                                                    ctcCGCTGCATAGTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATGTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAAc                                                                              <  2:2492704/149‑1 (MQ=255)
                                                                      ccGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACaa                                                                            <  2:2046811/149‑1 (MQ=255)
                                                                        gCTGCATATTCACCTCGGTGAGGTGATGTCGCGTGGTGAAAAAATGGTTTTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAAATGGATAGTGAACGTCTGAACaaga                                                                          <  2:557305/149‑1 (MQ=255)
                                                                        gCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACaaga                                                                          >  1:1231803/1‑149 (MQ=255)
                                                                          tGCATAGTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACaagaag                                                                        <  2:763007/149‑1 (MQ=255)
                                                                           gCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGc                                                                       <  1:1062460/149‑1 (MQ=255)
                                                                             atatTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCtt                                                                     >  1:1376423/1‑149 (MQ=255)
                                                                             atatTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCtt                                                                     <  1:2930529/149‑1 (MQ=255)
                                                                               atTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTca                                                                   <  2:894840/149‑1 (MQ=255)
                                                                                ttCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTcag                                                                  >  2:3660203/1‑149 (MQ=255)
                                                                                  cACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTcagca                                                                <  2:2746720/149‑1 (MQ=255)
                                                                                     cTCGGTCAGGTGATGTCGCGTGGTTAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTg                                                             <  1:2588107/149‑1 (MQ=255)
                                                                                       cGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGc                                                           <  2:3729066/149‑1 (MQ=255)
                                                                                        ggTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCa                                                          <  2:2700042/149‑1 (MQ=255)
                                                                                        ggTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCa                                                          <  2:2109740/149‑1 (MQ=255)
                                                                                            aGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCagaag                                                      >  1:833752/1‑149 (MQ=255)
                                                                                              gTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGAt                                                    >  2:3812336/1‑149 (MQ=255)
                                                                                              gTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGAt                                                    <  2:1828163/149‑1 (MQ=255)
                                                                                              gTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTGTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGAt                                                    <  2:42946/149‑1 (MQ=255)
                                                                                                gATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTg                                                  >  1:1595537/1‑149 (MQ=255)
                                                                                                   gTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCaa                                               <  1:1944577/149‑1 (MQ=255)
                                                                                                      gcgTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTTCAATtg                                            <  2:555919/149‑1 (MQ=255)
                                                                                                        gtggtgAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATtgct                                          <  1:2740553/149‑1 (MQ=255)
                                                                                                           gtgAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTATTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGcc                                       >  1:3411983/1‑149 (MQ=255)
                                                                                                              aaaaaaTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCtgt                                    >  2:594929/1‑149 (MQ=255)
                                                                                                               aaaaaTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCtgtg                                   <  1:1770754/149‑1 (MQ=255)
                                                                                                                aaaaTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGa                                  <  2:2871119/149‑1 (MQ=255)
                                                                                                                   aTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCa                               >  1:3627081/1‑149 (MQ=255)
                                                                                                                   aTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCa                               >  1:1581479/1‑149 (MQ=255)
                                                                                                                   aTGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCa                               >  1:891742/1‑149 (MQ=255)
                                                                                                                    tGGAATTTACTATCCAGACATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAg                              <  2:3351047/149‑1 (MQ=255)
                                                                                                                    tGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAg                              <  2:1300130/149‑1 (MQ=255)
                                                                                                                     ggAATTTACTATCCAGACATCGATCGAACGAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAgt                             <  1:2814475/149‑1 (MQ=255)
                                                                                                                     ggAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCCgt                             >  2:1277009/1‑149 (MQ=255)
                                                                                                                     ggAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAgt                             >  2:1094315/1‑149 (MQ=255)
                                                                                                                        aTTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGtgtg                          >  1:265608/1‑149 (MQ=255)
                                                                                                                         tttACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTgg                         <  2:2553823/149‑1 (MQ=255)
                                                                                                                         tttACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTgg                         <  1:1024766/149‑1 (MQ=255)
                                                                                                                              tATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTa                    <  2:1468552/149‑1 (MQ=255)
                                                                                                                              tATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTa                    <  1:636671/149‑1 (MQ=255)
                                                                                                                                tCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAAc                  >  1:1906265/1‑149 (MQ=255)
                                                                                                                                   aGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCgg               >  1:3324881/1‑149 (MQ=255)
                                                                                                                                    gAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCggg              <  1:2225869/149‑1 (MQ=255)
                                                                                                                                       atcgatcgAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGc           <  2:1456201/149‑1 (MQ=255)
                                                                                                                                       atcgatcgAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGc           <  2:924162/149‑1 (MQ=255)
                                                                                                                                        tcgatcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGcc          <  2:1776593/149‑1 (MQ=255)
                                                                                                                                        tcgatagaACTAGGTGTAAGCCTCATTACGCCACTATTTTCTGAGCGCTGCGGCGATAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGAAGAAGATTGCAATTTCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGcc          >  2:3383307/1‑149 (MQ=255)
                                                                                                                                          gatcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCgg        >  1:3891475/1‑149 (MQ=255)
                                                                                                                                           atcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACACGAAGCTTCAGCAGTGGCAGAAGATTGCACTTGCTGCCTGTGAGCCGTCTGGTCGTAACCGGGTGCCGGa       >  2:2091283/1‑149 (MQ=255)
                                                                                                                                           atcgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGa       >  2:3335604/1‑149 (MQ=255)
                                                                                                                                             cgaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCGGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGaaa     <  2:738487/149‑1 (MQ=255)
                                                                                                                                              gaACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAAt    >  1:12111/1‑149 (MQ=255)
                                                                                                                                               aaCTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATc   <  1:2928833/149‑1 (MQ=255)
                                                                                                                                               aaCTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATc   <  2:3018297/149‑1 (MQ=255)
                                                                                                                                                aCTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATcc  >  2:4007226/1‑149 (MQ=255)
                                                                                                                                                aCTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATcc  >  1:903066/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCC  >  CP009273/3084651‑3084943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCGTC  >  CP009273/3084649‑3084946
                                                                                                                                                     |                                                                                                                                                    
GAAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTA                                                                                                                                                      <  SRR19973068.1539835/150‑1 (MQ=60)
GAAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTA                                                                                                                                                      <  SRR19973068.1539875/150‑1 (MQ=60)
 AAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGACTTTACTATCCAGAAATCGATCGAACTAG                                                                                                                                                     >  SRR19973068.1627764/1‑150 (MQ=60)
   ATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGT                                                                                                                                                   >  SRR19973068.1684055/1‑150 (MQ=60)
        CAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAG                                                                                                                                              >  SRR19973068.418044/1‑150 (MQ=60)
         AGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGC                                                                                                                                             >  SRR19973068.2219993/1‑150 (MQ=60)
         AGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGC                                                                                                                                             >  SRR19973068.2219991/1‑150 (MQ=60)
           CGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGTCCAGTTCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTAGCGTGGTGAAAAAATGGAATTTACTATCCAGTAATCGATCGAACTAGGTGTAAGCCT                                                                                                                                           <  SRR19973068.302534/150‑1 (MQ=60)
              CAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAT                                                                                                                                        >  SRR19973068.3554171/1‑150 (MQ=60)
              CAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAT                                                                                                                                        <  SRR19973068.3554423/150‑1 (MQ=60)
              CAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAT                                                                                                                                        <  SRR19973068.3554713/150‑1 (MQ=60)
               AGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATT                                                                                                                                       <  SRR19973068.243588/150‑1 (MQ=60)
               AGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATT                                                                                                                                       <  SRR19973068.3035638/150‑1 (MQ=60)
               AGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATT                                                                                                                                       <  SRR19973068.3035784/150‑1 (MQ=60)
                GCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTA                                                                                                                                      <  SRR19973068.1462732/150‑1 (MQ=60)
                    ANAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCC                                                                                                                                  >  SRR19973068.3129048/1‑150 (MQ=60)
                    AAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCC                                                                                                                                  <  SRR19973068.921053/150‑1 (MQ=60)
                      ANAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCAC                                                                                                                                >  SRR19973068.2889003/1‑150 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                               <  SRR19973068.477151/150‑1 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                               >  SRR19973068.3889635/1‑150 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                               <  SRR19973068.1927908/150‑1 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                               <  SRR19973068.1927883/150‑1 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                               <  SRR19973068.478288/150‑1 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                               >  SRR19973068.2341916/1‑150 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                               <  SRR19973068.562351/150‑1 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACT                                                                                                                               >  SRR19973068.3889592/1‑150 (MQ=60)
                       AAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCAGTACGCCACT                                                                                                                               >  SRR19973068.3891766/1‑150 (MQ=60)
                              GAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCT                                                                                                                        >  SRR19973068.778492/1‑150 (MQ=60)
                               AAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTG                                                                                                                       >  SRR19973068.1386657/1‑150 (MQ=60)
                                 GTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAG                                                                                                                     <  SRR19973068.553414/150‑1 (MQ=60)
                                 GTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAG                                                                                                                     <  SRR19973068.2424000/150‑1 (MQ=60)
                                    AAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGC                                                                                                                  <  SRR19973068.2345614/150‑1 (MQ=60)
                                    AAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGC                                                                                                                  >  SRR19973068.2605000/1‑150 (MQ=60)
                                     AGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCT                                                                                                                 <  SRR19973068.2287904/150‑1 (MQ=60)
                                     AGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCT                                                                                                                 <  SRR19973068.735553/150‑1 (MQ=60)
                                     AGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCT                                                                                                                 <  SRR19973068.735520/150‑1 (MQ=60)
                                           TGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCG                                                                                                           >  SRR19973068.302520/1‑150 (MQ=60)
                                            GGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGT                                                                                                          >  SRR19973068.1069548/1‑150 (MQ=60)
                                               AGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAA                                                                                                       >  SRR19973068.3449172/1‑150 (MQ=60)
                                               AGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAA                                                                                                       >  SRR19973068.313777/1‑150 (MQ=60)
                                               AGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAA                                                                                                       >  SRR19973068.313734/1‑150 (MQ=60)
                                                  CCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACT                                                                                                    <  SRR19973068.1377146/150‑1 (MQ=60)
                                                         GACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGT                                                                                             <  SRR19973068.3817899/150‑1 (MQ=60)
                                                         GACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGT                                                                                             >  SRR19973068.2466360/1‑150 (MQ=60)
                                                         GACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGT                                                                                             >  SRR19973068.3057654/1‑150 (MQ=60)
                                                             ATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAAC                                                                                         <  SRR19973068.1690220/150‑1 (MQ=60)
                                                             ATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAAC                                                                                         <  SRR19973068.2636329/150‑1 (MQ=60)
                                                                 CGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCT                                                                                     >  SRR19973068.1511106/1‑150 (MQ=60)
                                                                 CGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCT                                                                                     >  SRR19973068.1511128/1‑150 (MQ=60)
                                                                   AATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGA                                                                                   <  SRR19973068.728307/150‑1 (MQ=60)
                                                                   AATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGA                                                                                   <  SRR19973068.2803172/150‑1 (MQ=60)
                                                                   AATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGA                                                                                   <  SRR19973068.2803301/150‑1 (MQ=60)
                                                                     TCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAAC                                                                                 <  SRR19973068.2193217/150‑1 (MQ=60)
                                                                         CGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGA                                                                             >  SRR19973068.1239987/1‑150 (MQ=60)
                                                                             GCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCT                                                                         <  SRR19973068.1069407/150‑1 (MQ=60)
                                                                             GCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACGAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCT                                                                         <  SRR19973068.1787789/150‑1 (MQ=60)
                                                                              CATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTT                                                                        >  SRR19973068.1385197/1‑150 (MQ=60)
                                                                               ATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTC                                                                       <  SRR19973068.2948733/150‑1 (MQ=60)
                                                                                TATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCA                                                                      >  SRR19973068.3555902/1‑150 (MQ=60)
                                                                                    CACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGTTTTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAG                                                                  >  SRR19973068.150182/1‑150 (MQ=60)
                                                                                    CACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAG                                                                  >  SRR19973068.152638/1‑150 (MQ=60)
                                                                                       CTGGGTCAGGTGTTGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGG                                                               <  SRR19973068.2604346/150‑1 (MQ=60)
                                                                                       CTCGGTCAGGTGATGTCGCGTGGTTAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGG                                                               <  SRR19973068.2604455/150‑1 (MQ=60)
                                                                                             CAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAG                                                         >  SRR19973068.839415/1‑150 (MQ=60)
                                                                                             CAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAG                                                         >  SRR19973068.2234712/1‑150 (MQ=60)
                                                                                                 TGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTG                                                     >  SRR19973068.1605933/1‑150 (MQ=60)
                                                                                                 TGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTG                                                     >  SRR19973068.1605997/1‑150 (MQ=60)
                                                                                                     GTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAAT                                                 <  SRR19973068.1957498/150‑1 (MQ=60)
                                                                                                     GTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAAT                                                 <  SRR19973068.1957499/150‑1 (MQ=60)
                                                                                                          GTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTG                                            <  SRR19973068.2757433/150‑1 (MQ=60)
                                                                                                            GGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCC                                          >  SRR19973068.3432596/1‑150 (MQ=60)
                                                                                                            GGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTATTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCC                                          >  SRR19973068.3432592/1‑150 (MQ=60)
                                                                                                              TGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTG                                        >  SRR19973068.133973/1‑150 (MQ=60)
                                                                                                              TGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTG                                        >  SRR19973068.3692981/1‑150 (MQ=60)
                                                                                                                 AAAAATGGAATTTACTATCCAGAAATCGATCGCACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGA                                     <  SRR19973068.1782230/150‑1 (MQ=60)
                                                                                                                 AAAAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGA                                     <  SRR19973068.782454/150‑1 (MQ=60)
                                                                                                                   AAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGC                                   >  SRR19973068.4062961/1‑150 (MQ=60)
                                                                                                                   AAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGC                                   >  SRR19973068.2014015/1‑150 (MQ=60)
                                                                                                                   AAATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGC                                   >  SRR19973068.2512160/1‑150 (MQ=60)
                                                                                                                    AATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCA                                  >  SRR19973068.897769/1‑150 (MQ=60)
                                                                                                                    AATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCA                                  >  SRR19973068.3649079/1‑150 (MQ=60)
                                                                                                                    AATGGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCA                                  >  SRR19973068.1591872/1‑150 (MQ=60)
                                                                                                                       GGAATTTACTATCCAGACATCGATCGAACGAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTG                               <  SRR19973068.2831979/150‑1 (MQ=60)
                                                                                                                       GGAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTG                               <  SRR19973068.3156937/150‑1 (MQ=60)
                                                                                                                        GAATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGT                              >  SRR19973068.1969834/1‑150 (MQ=60)
                                                                                                                         AATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTG                             >  SRR19973068.267104/1‑150 (MQ=60)
                                                                                                                         AATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTG                             >  SRR19973068.267486/1‑150 (MQ=60)
                                                                                                                         AATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTG                             >  SRR19973068.2317658/1‑150 (MQ=60)
                                                                                                                          ATTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGG                            >  SRR19973068.3430460/1‑150 (MQ=60)
                                                                                                                           TTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGT                           <  SRR19973068.1032130/150‑1 (MQ=60)
                                                                                                                           TTTACTATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGT                           <  SRR19973068.1031394/150‑1 (MQ=60)
                                                                                                                                TATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAA                      >  SRR19973068.90139/1‑150 (MQ=60)
                                                                                                                                TATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAA                      >  SRR19973068.90089/1‑150 (MQ=60)
                                                                                                                                TATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAA                      <  SRR19973068.640862/150‑1 (MQ=60)
                                                                                                                                 ATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAAC                     >  SRR19973068.688329/1‑150 (MQ=60)
                                                                                                                                 ATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAAC                     >  SRR19973068.1233136/1‑150 (MQ=60)
                                                                                                                                 ATCCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAAC                     >  SRR19973068.1918921/1‑150 (MQ=60)
                                                                                                                                   CCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCG                   >  SRR19973068.1736897/1‑150 (MQ=60)
                                                                                                                                   CCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCG                   >  SRR19973068.382203/1‑150 (MQ=60)
                                                                                                                                   CCAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCG                   >  SRR19973068.918077/1‑150 (MQ=60)
                                                                                                                                    CAGAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGG                  >  SRR19973068.3345184/1‑150 (MQ=60)
                                                                                                                                      GAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGT                <  SRR19973068.2240403/150‑1 (MQ=60)
                                                                                                                                      GAAATCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGATGCCTGTGAGCAGTGTGGTCGTAACCGGGT                <  SRR19973068.526398/150‑1 (MQ=60)
                                                                                                                                          TCGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCG            >  SRR19973068.2690899/1‑150 (MQ=60)
                                                                                                                                           CGATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGG           >  SRR19973068.3915008/1‑150 (MQ=60)
                                                                                                                                            GATCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGGGAGCAGTGTGGTCGTAACCGGGTGCCGGA          >  SRR19973068.1683631/1‑150 (MQ=60)
                                                                                                                                              TCGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAA        <  SRR19973068.52498/150‑1 (MQ=60)
                                                                                                                                               CGAACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAAT       >  SRR19973068.12299/1‑150 (MQ=60)
                                                                                                                                                 AACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCC     <  SRR19973068.2947040/150‑1 (MQ=60)
                                                                                                                                                 AACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCC     >  SRR19973068.909163/1‑150 (MQ=60)
                                                                                                                                                 AACTAGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCC     <  SRR19973068.2947029/150‑1 (MQ=60)
                                                                                                                                                    TAGGTGTAAGCCTCATTACGCCACTTTTTTCTCAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCGTC  >  SRR19973068.2147595/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GAAATTACCAGCGCCAGCAAAAAAAGCGTGGAAGTGAAGGTGCTGGAAGGCCAGATCGACGATCGCGAATCTCCGCTGCATATTCACCTCGGTCAGGTGATGTCGCGTGGTGAAAAAATGGAATTTACTATCCAGAAATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCGTC  >  CP009273/3084649‑3084946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑