Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,173,669 G→T 100% A189A (GCG→GCT)  ygiB → DUF1190 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,173,6690GT99.4% 540.1 / ‑4.2 168A189A (GCG→GCT) ygiBDUF1190 family protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (84/83);  total (85/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 168 total aligned reads displayed.

TTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGAC  >  CP009273/3173524‑3173812
                                                                                                                                                 |                                                                                                                                               
ttGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTac                                                                                                                                               >  2:1605341/1‑148 (MQ=255)
ttGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTac                                                                                                                                               >  1:1492186/1‑148 (MQ=255)
  gCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTacca                                                                                                                                             >  1:3382896/1‑148 (MQ=255)
      agcagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACcaccac                                                                                                                                         >  1:2077110/1‑148 (MQ=255)
       gcagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCACGGGCACCAAAACCGGCTACCACCACt                                                                                                                                        >  2:2111750/1‑148 (MQ=255)
        cagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTa                                                                                                                                       >  2:1163388/1‑148 (MQ=255)
             gCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAACACCGGCTACCACCACTACCGtt                                                                                                                                  >  2:1864328/1‑148 (MQ=255)
              cTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTa                                                                                                                                 >  2:2356217/1‑148 (MQ=255)
                gTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAcc                                                                                                                               >  1:548147/1‑148 (MQ=255)
                gTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAcc                                                                                                                               <  2:2832204/148‑1 (MQ=255)
                  tctcCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCg                                                                                                                             <  1:1323776/148‑1 (MQ=255)
                     cctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTgg                                                                                                                          >  1:1138585/1‑148 (MQ=255)
                      ctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGc                                                                                                                         >  2:1891793/1‑148 (MQ=255)
                       tcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCg                                                                                                                        >  2:73500/1‑148 (MQ=255)
                       tcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCg                                                                                                                        >  2:2780357/1‑148 (MQ=255)
                        cGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCgg                                                                                                                       <  1:2873765/148‑1 (MQ=255)
                          aaaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGtt                                                                                                                     >  1:196978/1‑148 (MQ=255)
                           aaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGttt                                                                                                                    >  1:1423554/1‑148 (MQ=255)
                                ccagccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTg                                                                                                               >  1:805056/1‑148 (MQ=255)
                                         ccGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTg                                                                                                      <  2:2369228/148‑1 (MQ=255)
                                          cGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACGACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGc                                                                                                     <  1:2273626/148‑1 (MQ=255)
                                             cTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcac                                                                                                  >  2:3181082/1‑147 (MQ=255)
                                              ttACGGTAAATATACCGACGCGACGGGTAAACACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaa                                                                                                 <  1:20442/148‑1 (MQ=255)
                                              ttACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaa                                                                                                 <  1:21297/148‑1 (MQ=255)
                                              ttACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaa                                                                                                 >  2:2718831/1‑148 (MQ=255)
                                              ttACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaa                                                                                                 >  2:2719532/1‑148 (MQ=255)
                                              ttACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaa                                                                                                 <  1:3473960/148‑1 (MQ=255)
                                              ttACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaa                                                                                                 <  1:3473854/148‑1 (MQ=255)
                                               tACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaac                                                                                                >  1:1031529/1‑148 (MQ=255)
                                               tACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaac                                                                                                >  1:1987685/1‑148 (MQ=255)
                                                aCGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaaca                                                                                               <  1:964884/148‑1 (MQ=255)
                                                 cGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaacaa                                                                                              <  2:2449462/148‑1 (MQ=255)
                                                 cGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaacaa                                                                                              <  2:1311852/148‑1 (MQ=255)
                                                    tAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGc                                                                                           <  1:1278326/148‑1 (MQ=255)
                                                     aaaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                                                                          <  2:1444789/148‑1 (MQ=255)
                                                      aaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCAc                                                                                         <  1:601732/148‑1 (MQ=255)
                                                      aaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCAc                                                                                         <  2:2689381/148‑1 (MQ=255)
                                                        tataCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTa                                                                                       <  1:3369911/148‑1 (MQ=255)
                                                          taCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                     >  2:1335977/1‑148 (MQ=255)
                                                          taCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                     >  2:1528283/1‑148 (MQ=255)
                                                          taCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                     >  2:1528406/1‑148 (MQ=255)
                                                          taCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                     <  2:954495/148‑1 (MQ=255)
                                                                       ggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCaa                                                                        <  1:1050803/148‑1 (MQ=255)
                                                                       ggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCaa                                                                        <  1:705893/148‑1 (MQ=255)
                                                                         tAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAcc                                                                      >  1:1835099/1‑148 (MQ=255)
                                                                            aaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGt                                                                   >  1:317171/1‑148 (MQ=255)
                                                                               cTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAcc                                                                <  1:212489/148‑1 (MQ=255)
                                                                                 aTGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACctc                                                              <  1:142387/148‑1 (MQ=255)
                                                                                  tGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACctct                                                             <  2:687484/148‑1 (MQ=255)
                                                                                   ggCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtctt                                                            <  2:2970066/148‑1 (MQ=255)
                                                                                   ggCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtctt                                                            <  2:2969996/148‑1 (MQ=255)
                                                                                    gcgcAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtcttc                                                           <  2:344917/148‑1 (MQ=255)
                                                                                     cgcAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtcttct                                                          <  1:2292032/148‑1 (MQ=255)
                                                                                     cgcAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtcttct                                                          <  2:1472556/148‑1 (MQ=255)
                                                                                       cagcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCg                                                        <  1:3121359/148‑1 (MQ=255)
                                                                                       cagcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCg                                                        <  1:2709730/148‑1 (MQ=255)
                                                                                         gcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt                                                      <  2:3397974/148‑1 (MQ=255)
                                                                                         gcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt                                                      >  1:797434/1‑148 (MQ=255)
                                                                                          cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                     >  2:1059410/1‑148 (MQ=255)
                                                                                          cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                     >  2:2600974/1‑148 (MQ=255)
                                                                                          cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                     >  2:2600888/1‑148 (MQ=255)
                                                                                          cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                     >  2:2838951/1‑148 (MQ=255)
                                                                                           ccagccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCa                                                    <  1:1817529/148‑1 (MQ=255)
                                                                                             agccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAAt                                                  <  2:1423554/148‑1 (MQ=255)
                                                                                              gccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATg                                                 >  2:315847/1‑148 (MQ=255)
                                                                                               ccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCACCCGGTACCTCTTCTCGTTCAATgg                                                >  1:949608/1‑148 (MQ=255)
                                                                                                cagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATggg                                               >  1:3514998/1‑148 (MQ=255)
                                                                                                  ggCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTg                                             >  1:2520009/1‑148 (MQ=255)
                                                                                                    ccGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGc                                           <  2:10447/148‑1 (MQ=255)
                                                                                                       cACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGa                                        <  1:828509/148‑1 (MQ=255)
                                                                                                       cACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGa                                        <  1:828162/148‑1 (MQ=255)
                                                                                                         ccATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATa                                      >  1:727201/1‑148 (MQ=255)
                                                                                                          cATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAc                                     <  1:2354007/148‑1 (MQ=255)
                                                                                                          cATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAc                                     <  2:548381/148‑1 (MQ=255)
                                                                                                           aTGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAcc                                    >  2:827998/1‑148 (MQ=255)
                                                                                                           aTGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAcc                                    >  2:2966386/1‑148 (MQ=255)
                                                                                                            tGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCg                                   <  2:1950132/148‑1 (MQ=255)
                                                                                                             gACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCACTGGGTGGCTGATACCGa                                  >  2:45614/1‑148 (MQ=255)
                                                                                                              aCCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGAt                                 >  2:1926754/1‑148 (MQ=255)
                                                                                                               ccGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACTACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATg                                >  1:1609031/1‑148 (MQ=255)
                                                                                                                  tACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCACCCGGTACCGCTGCTCGTTCAATGGGGGGCTGAGCCCGAggggg                             >  1:3437724/1‑146 (MQ=255)
                                                                                                                   aCCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCACCCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGaaa                            >  1:3282233/1‑148 (MQ=255)
                                                                                                                     cGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAaga                          >  1:275202/1‑148 (MQ=255)
                                                                                                                         gACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCa                      >  2:328029/1‑148 (MQ=255)
                                                                                                                         gACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCa                      >  1:624815/1‑148 (MQ=255)
                                                                                                                              cAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCGCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTAtt                 >  1:886212/1‑148 (MQ=255)
                                                                                                                                aTGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAc               >  1:125860/1‑148 (MQ=255)
                                                                                                                                aTGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAc               <  1:2966386/148‑1 (MQ=255)
                                                                                                                                  ggCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCg             >  1:2149238/1‑148 (MQ=255)
                                                                                                                                   gCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGa            <  2:3551/148‑1 (MQ=255)
                                                                                                                                    cACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAg           <  1:2977757/148‑1 (MQ=255)
                                                                                                                                      ccAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAgcg         >  1:1634898/1‑148 (MQ=255)
                                                                                                                                       cAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAgcgc        <  1:785683/148‑1 (MQ=255)
                                                                                                                                        aaaaCCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGcc       >  2:1157367/1‑148 (MQ=255)
                                                                                                                                          aaCCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTCCCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCg     >  2:3082052/1‑148 (MQ=255)
                                                                                                                                            ccGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGa   <  2:3132558/148‑1 (MQ=255)
                                                                                                                                            ccGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGa   >  1:1596075/1‑148 (MQ=255)
                                                                                                                                            ccGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGa   <  2:2555148/148‑1 (MQ=255)
                                                                                                                                            ccGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGa   >  2:1776326/1‑148 (MQ=255)
                                                                                                                                             cGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGAc  <  2:1031529/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGAC  >  CP009273/3173524‑3173812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGT  >  CP009273/3173520‑3173818
                                                                                                                                                     |                                                                                                                                                     
GGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCT                                                                                                                                                       <  SRR19973066.1788740/150‑1 (MQ=60)
 GATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTA                                                                                                                                                      >  SRR19973066.2544951/1‑150 (MQ=60)
  ATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTAC                                                                                                                                                     >  SRR19973066.1502620/1‑150 (MQ=60)
    TTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCA                                                                                                                                                   >  SRR19973066.3405462/1‑150 (MQ=60)
    TTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCA                                                                                                                                                   >  SRR19973066.1628320/1‑150 (MQ=60)
        ACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCAC                                                                                                                                               >  SRR19973066.2091463/1‑150 (MQ=60)
               CCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTT                                                                                                                                        <  SRR19973066.1999674/150‑1 (MQ=60)
                  CTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACC                                                                                                                                     >  SRR19973066.552092/1‑150 (MQ=60)
                  CTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACC                                                                                                                                     >  SRR19973066.552328/1‑150 (MQ=60)
                      TCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTG                                                                                                                                 <  SRR19973066.3468748/150‑1 (MQ=60)
                      TCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTG                                                                                                                                 <  SRR19973066.1333372/150‑1 (MQ=60)
                      TCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTG                                                                                                                                 <  SRR19973066.1331841/150‑1 (MQ=60)
                       CTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGG                                                                                                                                <  SRR19973066.318942/150‑1 (MQ=60)
                       CTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGG                                                                                                                                >  SRR19973066.1146732/1‑150 (MQ=60)
                            CGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTT                                                                                                                           <  SRR19973066.2893031/150‑1 (MQ=60)
                            CGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTT                                                                                                                           >  SRR19973066.198665/1‑150 (MQ=60)
                             GAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTT                                                                                                                          >  SRR19973066.1433733/1‑150 (MQ=60)
                              AAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTT                                                                                                                         >  SRR19973066.3404895/1‑150 (MQ=60)
                               AAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTG                                                                                                                        >  SRR19973066.2640134/1‑150 (MQ=60)
                               AAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTG                                                                                                                        <  SRR19973066.3052581/150‑1 (MQ=60)
                               AAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTG                                                                                                                        >  SRR19973066.3538960/1‑150 (MQ=60)
                                  ACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGACGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTG                                                                                                                     >  SRR19973066.1563452/1‑150 (MQ=60)
                                  ACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTG                                                                                                                     >  SRR19973066.810896/1‑150 (MQ=60)
                                  ACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTG                                                                                                                     >  SRR19973066.810969/1‑150 (MQ=60)
                                   CCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGA                                                                                                                    <  SRR19973066.1656540/150‑1 (MQ=60)
                                   CCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGA                                                                                                                    <  SRR19973066.1141726/150‑1 (MQ=60)
                                    CCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAA                                                                                                                   >  SRR19973066.1950775/1‑150 (MQ=60)
                                              CGGCTTACGGTAAATATCCCGACGCGACGGGTAAAAACTCTGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACGACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCA                                                                                                         <  SRR19973066.2289252/150‑1 (MQ=60)
                                                 CTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAAC                                                                                                      >  SRR19973066.1039121/1‑150 (MQ=60)
                                                 CTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAAC                                                                                                      >  SRR19973066.2001693/1‑150 (MQ=60)
                                                  TTACGGTAAATATACCGACGCGACGGGTAAACACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACA                                                                                                     <  SRR19973066.20747/150‑1 (MQ=60)
                                                  TTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACA                                                                                                     <  SRR19973066.3497157/150‑1 (MQ=60)
                                                  TTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACA                                                                                                     <  SRR19973066.21614/150‑1 (MQ=60)
                                                  TTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACA                                                                                                     <  SRR19973066.3497050/150‑1 (MQ=60)
                                                  TTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACA                                                                                                     <  SRR19973066.3497153/150‑1 (MQ=60)
                                                    ACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTGCCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAA                                                                                                   <  SRR19973066.972015/150‑1 (MQ=60)
                                                    ACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAA                                                                                                   <  SRR19973066.971973/150‑1 (MQ=60)
                                                        TAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCAC                                                                                               <  SRR19973066.723860/150‑1 (MQ=60)
                                                        TAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCAC                                                                                               <  SRR19973066.1287558/150‑1 (MQ=60)
                                                          AATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTA                                                                                             <  SRR19973066.605869/150‑1 (MQ=60)
                                                            TATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATG                                                                                           >  SRR19973066.2075569/1‑150 (MQ=60)
                                                            TATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATG                                                                                           <  SRR19973066.3392389/150‑1 (MQ=60)
                                                               ACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAG                                                                                        <  SRR19973066.858390/150‑1 (MQ=60)
                                                                          GGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAC                                                                             <  SRR19973066.2847136/150‑1 (MQ=60)
                                                                           GGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACC                                                                            >  SRR19973066.1848206/1‑150 (MQ=60)
                                                                           GGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACC                                                                            <  SRR19973066.2232860/150‑1 (MQ=60)
                                                                           GGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACC                                                                            <  SRR19973066.1058529/150‑1 (MQ=60)
                                                                           GGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACC                                                                            <  SRR19973066.711111/150‑1 (MQ=60)
                                                                              AAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGT                                                                         >  SRR19973066.319546/1‑150 (MQ=60)
                                                                                   CTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTC                                                                    <  SRR19973066.214247/150‑1 (MQ=60)
                                                                                     ATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTT                                                                  <  SRR19973066.143758/150‑1 (MQ=60)
                                                                                         CGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCG                                                              <  SRR19973066.2307747/150‑1 (MQ=60)
                                                                                           CAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTT                                                            <  SRR19973066.3142202/150‑1 (MQ=60)
                                                                                           CAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTT                                                            >  SRR19973066.803286/1‑150 (MQ=60)
                                                                                           CAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTT                                                            <  SRR19973066.2727921/150‑1 (MQ=60)
                                                                                              CCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAA                                                         <  SRR19973066.2334316/150‑1 (MQ=60)
                                                                                               CCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAAT                                                        <  SRR19973066.1830478/150‑1 (MQ=60)
                                                                                                 AGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCACCCGGTACCTCTTCTCGTTCAATGG                                                      >  SRR19973066.956547/1‑150 (MQ=60)
                                                                                                  GCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGG                                                     >  SRR19973066.3538407/1‑150 (MQ=60)
                                                                                                    CAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTG                                                   >  SRR19973066.2537147/1‑150 (MQ=60)
                                                                                                     AGGCCGCACCATGACCGTACCGAAGACGGCAATTGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGAAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGG                                                  >  SRR19973066.658091/1‑150 (MQ=60)
                                                                                                     AGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGG                                                  <  SRR19973066.133834/150‑1 (MQ=60)
                                                                                                           CACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATA                                            >  SRR19973066.732589/1‑150 (MQ=60)
                                                                                                           CACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATA                                            <  SRR19973066.15048/150‑1 (MQ=60)
                                                                                                           CACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATA                                            <  SRR19973066.834204/150‑1 (MQ=60)
                                                                                                           CACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATA                                            <  SRR19973066.834313/150‑1 (MQ=60)
                                                                                                           CACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATA                                            <  SRR19973066.834555/150‑1 (MQ=60)
                                                                                                              CATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCG                                         <  SRR19973066.2369992/150‑1 (MQ=60)
                                                                                                                 GACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACTACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATG                                      >  SRR19973066.1620705/1‑150 (MQ=60)
                                                                                                                    CGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCACCCGGTACCGCTGCTCGTTCAATGGGGGGCTGAgcccgaggggg                                   >  SRR19973066.3460723/1‑139 (MQ=60)
                                                                                                                    CGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAA                                   >  SRR19973066.795550/1‑150 (MQ=60)
                                                                                                                    CGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAA                                   >  SRR19973066.2958007/1‑150 (MQ=60)
                                                                                                                     GTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCACCCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAA                                  >  SRR19973066.3303821/1‑150 (MQ=60)
                                                                                                                      TACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAG                                 <  SRR19973066.213727/150‑1 (MQ=60)
                                                                                                                       ACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGA                                >  SRR19973066.277222/1‑150 (MQ=60)
                                                                                                                           AAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCA                            >  SRR19973066.629274/1‑150 (MQ=60)
                                                                                                                               CGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTAT                        <  SRR19973066.3307273/150‑1 (MQ=60)
                                                                                                                                GGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATT                       >  SRR19973066.2454605/1‑150 (MQ=60)
                                                                                                                                GGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATT                       >  SRR19973066.892518/1‑150 (MQ=60)
                                                                                                                                GGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCGCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATT                       >  SRR19973066.892507/1‑150 (MQ=60)
                                                                                                                                  CAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAC                     >  SRR19973066.127025/1‑150 (MQ=60)
                                                                                                                                  CAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAC                     >  SRR19973066.127109/1‑150 (MQ=60)
                                                                                                                                    ATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCG                   <  SRR19973066.2986010/150‑1 (MQ=60)
                                                                                                                                    ATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCG                   >  SRR19973066.2162718/1‑150 (MQ=60)
                                                                                                                                    ATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCG                   >  SRR19973066.2164158/1‑150 (MQ=60)
                                                                                                                                        CACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCG               <  SRR19973066.2997434/150‑1 (MQ=60)
                                                                                                                                        CACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCG               >  SRR19973066.1646784/1‑150 (MQ=60)
                                                                                                                                           CAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCC            <  SRR19973066.791478/150‑1 (MQ=60)
                                                                                                                                              AACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGA         >  SRR19973066.1607634/1‑150 (MQ=60)
                                                                                                                                                     TACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTCCCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGT  >  SRR19973066.490492/1‑150 (MQ=60)
                                                                                                                                                     TACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGT  >  SRR19973066.955804/1‑150 (MQ=60)
                                                                                                                                                     TACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGT  >  SRR19973066.955977/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGT  >  CP009273/3173520‑3173818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑