Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,173,669 G→T 100% A189A (GCG→GCT)  ygiB → DUF1190 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,173,6690GT100.0% 556.7 / NA 168A189A (GCG→GCT) ygiBDUF1190 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (77/91);  total (77/91)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 168 total aligned reads displayed.

CCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGAC  >  CP009273/3173535‑3173812
                                                                                                                                      |                                                                                                                                               
ccGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCg                                                                                                                                    >  1:2271353/1‑148 (MQ=255)
 cGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGt                                                                                                                                   <  2:20721/148‑1 (MQ=255)
    tGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAc                                                                                                                                >  2:1487548/1‑148 (MQ=255)
    tGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAc                                                                                                                                >  2:1487174/1‑148 (MQ=255)
      ttCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAccc                                                                                                                              <  2:730152/148‑1 (MQ=255)
      ttCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAccc                                                                                                                              >  2:3044262/1‑148 (MQ=255)
      ttCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAccc                                                                                                                              >  2:3042674/1‑148 (MQ=255)
      ttCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAccc                                                                                                                              >  2:125932/1‑148 (MQ=255)
       tctcCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCg                                                                                                                             <  1:2310110/148‑1 (MQ=255)
       tctcCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCg                                                                                                                             <  2:636254/148‑1 (MQ=255)
        ctcctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGt                                                                                                                            <  2:662590/148‑1 (MQ=255)
        ctcctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGt                                                                                                                            >  2:2958532/1‑148 (MQ=255)
              gAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGt                                                                                                                      <  2:2606749/148‑1 (MQ=255)
               aaaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGtt                                                                                                                     <  2:1117345/148‑1 (MQ=255)
                aaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGttt                                                                                                                    >  1:2361033/1‑148 (MQ=255)
                   aCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTgg                                                                                                                 <  1:3058184/148‑1 (MQ=255)
                   aCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTgg                                                                                                                 <  1:2846369/148‑1 (MQ=255)
                    cccAGCCAGTCCGGCTTACGGTAAATATCCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGt                                                                                                                <  1:1486165/148‑1 (MQ=255)
                    cccAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGt                                                                                                                >  1:21/1‑148 (MQ=255)
                    cccAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGt                                                                                                                <  1:703079/148‑1 (MQ=255)
                       agccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGaa                                                                                                             <  2:1777631/148‑1 (MQ=255)
                        gccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAAt                                                                                                            <  2:2086651/148‑1 (MQ=255)
                        gccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAAt                                                                                                            <  1:1268229/148‑1 (MQ=255)
                        gccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAAt                                                                                                            <  1:1589196/148‑1 (MQ=255)
                         ccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATc                                                                                                           >  2:2905234/1‑148 (MQ=255)
                          cagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCt                                                                                                          <  2:438237/148‑1 (MQ=255)
                           agTCGGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTg                                                                                                         <  1:723371/148‑1 (MQ=255)
                           agTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTg                                                                                                         <  1:2166174/148‑1 (MQ=255)
                           agTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTg                                                                                                         <  1:517081/148‑1 (MQ=255)
                             tCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGtt                                                                                                       >  1:3442424/1‑148 (MQ=255)
                             tCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGtt                                                                                                       >  2:3021178/1‑148 (MQ=255)
                               cGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGc                                                                                                     <  1:3318623/148‑1 (MQ=255)
                                 gCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCca                                                                                                   >  1:3324463/1‑148 (MQ=255)
                                 gCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCca                                                                                                   >  2:2781472/1‑148 (MQ=255)
                                  cTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaa                                                                                                  >  2:1044038/1‑148 (MQ=255)
                                  cTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaa                                                                                                  >  2:2055051/1‑148 (MQ=255)
                                     aCGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCCCCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaaca                                                                                               >  2:1127830/1‑148 (MQ=255)
                                        gTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCAAGCCAGGCCGCACCAAGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAg                                                                                            >  1:2453201/1‑148 (MQ=255)
                                         tAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGc                                                                                           <  2:867359/148‑1 (MQ=255)
                                         tAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGc                                                                                           >  2:3319855/1‑148 (MQ=255)
                                          aaaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                                                                          >  1:2331362/1‑148 (MQ=255)
                                          aaaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                                                                          <  1:1922773/148‑1 (MQ=255)
                                          aaaTATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                                                                          <  1:1508618/148‑1 (MQ=255)
                                             tataCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTa                                                                                       >  1:903416/1‑148 (MQ=255)
                                               taCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGACAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                     >  1:214413/1‑148 (MQ=255)
                                                         acgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTg                                                                           <  1:588689/148‑1 (MQ=255)
                                                          cgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGc                                                                          >  2:1569758/1‑148 (MQ=255)
                                                           gggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCa                                                                         <  1:136981/148‑1 (MQ=255)
                                                            ggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCaa                                                                        <  1:2714773/148‑1 (MQ=255)
                                                            ggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCaa                                                                        <  1:82008/148‑1 (MQ=255)
                                                            ggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCaa                                                                        <  1:819131/148‑1 (MQ=255)
                                                             gTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAc                                                                       <  2:2113651/148‑1 (MQ=255)
                                                             gTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAc                                                                       <  2:462865/148‑1 (MQ=255)
                                                                aaaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCgg                                                                    >  2:2110805/1‑148 (MQ=255)
                                                                aaaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCgg                                                                    >  2:1413671/1‑148 (MQ=255)
                                                                 aaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGt                                                                   <  1:2298669/148‑1 (MQ=255)
                                                                 aaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGt                                                                   <  1:2298729/148‑1 (MQ=255)
                                                                  aaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTa                                                                  >  2:2308871/1‑148 (MQ=255)
                                                                   aCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAc                                                                 <  2:2735635/148‑1 (MQ=255)
                                                                   aCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAc                                                                 >  1:3497897/1‑148 (MQ=255)
                                                                   aCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAc                                                                 <  2:1903382/148‑1 (MQ=255)
                                                                   aCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGCGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAc                                                                 <  2:2736850/148‑1 (MQ=255)
                                                                    cTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAcc                                                                >  2:3219246/1‑148 (MQ=255)
                                                                    cTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAcc                                                                >  2:2292590/1‑148 (MQ=255)
                                                                    cTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAcc                                                                >  2:961240/1‑148 (MQ=255)
                                                                      aTGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACctc                                                              <  1:199888/148‑1 (MQ=255)
                                                                           gcAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTtctc                                                         <  1:2515474/148‑1 (MQ=255)
                                                                               cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                     <  2:2453825/148‑1 (MQ=255)
                                                                               cccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                     >  2:1476167/1‑148 (MQ=255)
                                                                                ccagccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCa                                                    <  2:2553957/148‑1 (MQ=255)
                                                                                 cagccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCaa                                                   <  1:1047907/148‑1 (MQ=255)
                                                                                      agGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGt                                              >  1:1948181/1‑148 (MQ=255)
                                                                                           gCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTg                                         <  2:2115038/148‑1 (MQ=255)
                                                                                              ccATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATa                                      >  1:1705994/1‑148 (MQ=255)
                                                                                              ccATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATa                                      >  1:1706055/1‑148 (MQ=255)
                                                                                               cATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAc                                     <  2:1675591/148‑1 (MQ=255)
                                                                                                 tGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCg                                   >  2:1720595/1‑148 (MQ=255)
                                                                                                   aCCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGAt                                 >  2:3142730/1‑148 (MQ=255)
                                                                                                     cGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATgg                               <  1:3042674/148‑1 (MQ=255)
                                                                                                       tACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGaa                             <  2:404166/148‑1 (MQ=255)
                                                                                                        aCCGAAGACGGCCATGGCACCAAAACCGGCTCCCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAACGCACTATGCAGCGTAGTGCCACCGGTCCCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGaca                            >  1:517547/1‑146 (MQ=255)
                                                                                                           gAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAagag                         <  2:2161750/148‑1 (MQ=255)
                                                                                                              gACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCa                      >  2:3305536/1‑148 (MQ=255)
                                                                                                                 ggCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTa                   <  1:3021178/148‑1 (MQ=255)
                                                                                                                   cAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTAtt                 <  2:1223672/148‑1 (MQ=255)
                                                                                                                   cAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTAtt                 <  1:2781389/148‑1 (MQ=255)
                                                                                                                    aaTGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTa                <  2:30/148‑1 (MQ=255)
                                                                                                                    aaTGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTa                <  1:896320/148‑1 (MQ=255)
                                                                                                                      tGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAcc              >  1:72884/1‑148 (MQ=255)
                                                                                                                      tGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAcc              <  2:2020568/148‑1 (MQ=255)
                                                                                                                        gCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGa            >  1:3496968/1‑148 (MQ=255)
                                                                                                                           ccAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGAAACCGGTAACTCTTCTCGTTAAATGGGTGGCTGATACCGATGGAAAGAGTCAGGATTACCGAgcg         >  1:2182356/1‑148 (MQ=255)
                                                                                                                            cAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAgcgc        >  1:993075/1‑148 (MQ=255)
                                                                                                                            cAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAgcgc        >  1:220855/1‑148 (MQ=255)
                                                                                                                                aCCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAACGAGTCAGTATTACCGAGCGCCCgg    >  2:259626/1‑148 (MQ=255)
                                                                                                                                aCCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCgg    >  2:3196055/1‑148 (MQ=255)
                                                                                                                                aCCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCgg    >  1:894638/1‑148 (MQ=255)
                                                                                                                                 ccGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGa   >  1:389487/1‑148 (MQ=255)
                                                                                                                                 ccGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGa   >  2:2710139/1‑148 (MQ=255)
                                                                                                                                  cGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGAc  <  1:2056628/148‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                               
CCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGAC  >  CP009273/3173535‑3173812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCG  >  CP009273/3173520‑3173817
                                                                                                                                                     |                                                                                                                                                    
GGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCT                                                                                                                                                      <  SRR19973065.3010093/150‑1 (MQ=60)
GGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCT                                                                                                                                                      <  SRR19973065.2563747/150‑1 (MQ=60)
GGATTTGAACAGAAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCT                                                                                                                                                      <  SRR19973065.2417665/150‑1 (MQ=60)
             AGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCG                                                                                                                                         >  SRR19973065.2285698/1‑150 (MQ=60)
             AGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCG                                                                                                                                         >  SRR19973065.2285756/1‑150 (MQ=60)
                   TGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCC                                                                                                                                   >  SRR19973065.2569936/1‑150 (MQ=60)
                      TCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTG                                                                                                                                <  SRR19973065.2324620/150‑1 (MQ=60)
                             GAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTT                                                                                                                         >  SRR19973065.2318254/1‑150 (MQ=60)
                             GAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTT                                                                                                                         >  SRR19973065.1482939/1‑150 (MQ=60)
                             GAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTT                                                                                                                         >  SRR19973065.2375717/1‑150 (MQ=60)
                                 AACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGT                                                                                                                     >  SRR19973065.21/1‑150 (MQ=60)
                                  ACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTG                                                                                                                    <  SRR19973065.3076877/150‑1 (MQ=60)
                                  ACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTG                                                                                                                    <  SRR19973065.2863883/150‑1 (MQ=60)
                                   CCCAGCCAGTCCGGCTTACGGTAAATATCCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGA                                                                                                                   <  SRR19973065.1495650/150‑1 (MQ=60)
                                   CCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGA                                                                                                                   <  SRR19973065.707754/150‑1 (MQ=60)
                                       GCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCT                                                                                                               <  SRR19973065.1599803/150‑1 (MQ=60)
                                       GCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCT                                                                                                               <  SRR19973065.1276654/150‑1 (MQ=60)
                                       GCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCT                                                                                                               <  SRR19973065.1276596/150‑1 (MQ=60)
                                          AGTCGGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTT                                                                                                            <  SRR19973065.728216/150‑1 (MQ=60)
                                          AGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTT                                                                                                            <  SRR19973065.520499/150‑1 (MQ=60)
                                          AGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTT                                                                                                            <  SRR19973065.2448122/150‑1 (MQ=60)
                                          AGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTT                                                                                                            >  SRR19973065.3463415/1‑150 (MQ=60)
                                          AGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTT                                                                                                            >  SRR19973065.1001027/1‑150 (MQ=60)
                                          AGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTT                                                                                                            <  SRR19973065.2180092/150‑1 (MQ=60)
                                          AGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACGACCGTTACCCGTGGCGGTTTTGGTGAATCTGTT                                                                                                            <  SRR19973065.398225/150‑1 (MQ=60)
                                              CGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCA                                                                                                        <  SRR19973065.3338868/150‑1 (MQ=60)
                                              CGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCA                                                                                                        >  SRR19973065.3344751/1‑150 (MQ=60)
                                              CGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCA                                                                                                        >  SRR19973065.3344793/1‑150 (MQ=60)
                                                     CGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCAAGCCAGGCCGCACCAAGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAG                                                                                                 >  SRR19973065.2468660/1‑150 (MQ=60)
                                                       GTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCA                                                                                               >  SRR19973065.2345939/1‑150 (MQ=60)
                                                         AAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACT                                                                                             <  SRR19973065.1935381/150‑1 (MQ=60)
                                                         AAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACT                                                                                             <  SRR19973065.1936788/150‑1 (MQ=60)
                                                         AAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACT                                                                                             <  SRR19973065.1518429/150‑1 (MQ=60)
                                                          AATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTA                                                                                            >  SRR19973065.909230/1‑150 (MQ=60)
                                                            TATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGACAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATG                                                                                          >  SRR19973065.216031/1‑150 (MQ=60)
                                                                 CGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCG                                                                                     <  SRR19973065.52458/150‑1 (MQ=60)
                                                                  GACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGT                                                                                    >  SRR19973065.2244918/1‑150 (MQ=60)
                                                                    CGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAG                                                                                  <  SRR19973065.2865628/150‑1 (MQ=60)
                                                                     GCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGT                                                                                 <  SRR19973065.2074143/150‑1 (MQ=60)
                                                                      CGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTG                                                                                <  SRR19973065.928323/150‑1 (MQ=60)
                                                                        ACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCA                                                                              <  SRR19973065.592342/150‑1 (MQ=60)
                                                                          GGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAC                                                                            <  SRR19973065.138153/150‑1 (MQ=60)
                                                                           GGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACC                                                                           <  SRR19973065.824514/150‑1 (MQ=60)
                                                                           GGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACC                                                                           <  SRR19973065.82822/150‑1 (MQ=60)
                                                                           GGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACC                                                                           <  SRR19973065.82872/150‑1 (MQ=60)
                                                                           GGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACC                                                                           <  SRR19973065.2731660/150‑1 (MQ=60)
                                                                              AAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGT                                                                        <  SRR19973065.2180392/150‑1 (MQ=60)
                                                                                AAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAC                                                                      >  SRR19973065.3519149/1‑150 (MQ=60)
                                                                                AAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAC                                                                      <  SRR19973065.2313198/150‑1 (MQ=60)
                                                                                AAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAC                                                                      <  SRR19973065.2313138/150‑1 (MQ=60)
                                                                                AAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAC                                                                      >  SRR19973065.2175592/1‑150 (MQ=60)
                                                                                    TATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCT                                                                  >  SRR19973065.3493557/1‑150 (MQ=60)
                                                                                     ATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTT                                                                 <  SRR19973065.201434/150‑1 (MQ=60)
                                                                                          GCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGT                                                            <  SRR19973065.2531277/150‑1 (MQ=60)
                                                                                          GCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGT                                                            <  SRR19973065.2531135/150‑1 (MQ=60)
                                                                                           CAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTT                                                           <  SRR19973065.775094/150‑1 (MQ=60)
                                                                                            AGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTC                                                          >  SRR19973065.2340149/1‑150 (MQ=60)
                                                                                                CAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATG                                                      <  SRR19973065.1054839/150‑1 (MQ=60)
                                                                                                   CCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGT                                                   >  SRR19973065.1960916/1‑150 (MQ=60)
                                                                                                     AGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGG                                                 <  SRR19973065.617784/150‑1 (MQ=60)
                                                                                                           CACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATA                                           <  SRR19973065.1580197/150‑1 (MQ=60)
                                                                                                           CACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATA                                           >  SRR19973065.1717351/1‑150 (MQ=60)
                                                                                                           CACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATA                                           >  SRR19973065.1717412/1‑150 (MQ=60)
                                                                                                                  ACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGG                                    <  SRR19973065.1874205/150‑1 (MQ=60)
                                                                                                                    CGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAA                                  <  SRR19973065.3062892/150‑1 (MQ=60)
                                                                                                                    CGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAA                                  <  SRR19973065.3529614/150‑1 (MQ=60)
                                                                                                                    CGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAA                                  <  SRR19973065.3061295/150‑1 (MQ=60)
                                                                                                                     GTACCGAAGACGGCCATGGCACCAAAACCGGCTCCCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAACGCACTATGCAGCGTAGTGCCACCGGTCCCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGACA                                 >  SRR19973065.520966/1‑150 (MQ=60)
                                                                                                                      TACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAG                                <  SRR19973065.3181312/150‑1 (MQ=60)
                                                                                                                                GGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATT                      <  SRR19973065.3039689/150‑1 (MQ=60)
                                                                                                                                  CAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAC                    <  SRR19973065.2798646/150‑1 (MQ=60)
                                                                                                                                  CAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAC                    <  SRR19973065.2798730/150‑1 (MQ=60)
                                                                                                                                   AATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACC                   <  SRR19973065.902037/150‑1 (MQ=60)
                                                                                                                                   AATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACC                   >  SRR19973065.73459/1‑150 (MQ=60)
                                                                                                                                   AATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACC                   >  SRR19973065.73645/1‑150 (MQ=60)
                                                                                                                                     TGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGA                 >  SRR19973065.3518217/1‑150 (MQ=60)
                                                                                                                                        CACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGAAACCGGTAACTCTTCTCGTTAAATGGGTGGCTGATACCGATGGAAAGAGTCAGGATTACCGAGCG              >  SRR19973065.2196356/1‑150 (MQ=60)
                                                                                                                                         ACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGC             >  SRR19973065.999675/1‑150 (MQ=60)
                                                                                                                                         ACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGC             >  SRR19973065.222498/1‑150 (MQ=60)
                                                                                                                                             AAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGG         >  SRR19973065.900324/1‑150 (MQ=60)
                                                                                                                                             AAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCGTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCCGTCTTACCGAGCGCCCGG         >  SRR19973065.900417/1‑150 (MQ=60)
                                                                                                                                              AACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGA        >  SRR19973065.392209/1‑150 (MQ=60)
                                                                                                                                                CCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTAGGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACT      <  SRR19973065.1655772/150‑1 (MQ=60)
                                                                                                                                                 CGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTG     <  SRR19973065.2068174/150‑1 (MQ=60)
                                                                                                                                                 CGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTG     <  SRR19973065.2069758/150‑1 (MQ=60)
                                                                                                                                                   GCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGATGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGC   <  SRR19973065.3062031/150‑1 (MQ=60)
                                                                                                                                                    CTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCG  >  SRR19973065.2772140/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCG  >  CP009273/3173520‑3173817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑