Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,249,474 C→A 52.0% T179T (ACG→ACT)  yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,249,4740CA52.0% 61.6 / inf 215T179T (ACG→ACT) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base C (51/52);  new base A (64/48);  total (115/100)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 217 total aligned reads displayed.

CCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCG  >  CP009273/3249328‑3249620
                                                                                                                                                  |                                                                                                                                                  
 cAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGt                                                                                                                                                  <  2:2010931/148‑1 (MQ=255)
 cAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGt                                                                                                                                                  <  2:3468898/148‑1 (MQ=255)
 cAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGt                                                                                                                                                  <  2:1512857/148‑1 (MQ=255)
  aaTATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTc                                                                                                                                                 <  1:3329515/148‑1 (MQ=255)
  aaTATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTc                                                                                                                                                 <  1:1562736/148‑1 (MQ=255)
    tatGCAGCCCCCAATTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAATCGGATCGCCGCATACGGGACTTCATTGACTAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAg                                                                                                                                               <  1:745196/148‑1 (MQ=255)
      tGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCg                                                                                                                                             >  2:348356/1‑148 (MQ=255)
      tGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCg                                                                                                                                             >  2:348471/1‑148 (MQ=255)
           cccccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGAc                                                                                                                                        <  2:2128741/148‑1 (MQ=255)
               cACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCtt                                                                                                                                    >  1:2495393/1‑148 (MQ=255)
               cACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCtt                                                                                                                                    >  1:2358923/1‑148 (MQ=255)
                aCTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTg                                                                                                                                   <  2:117446/148‑1 (MQ=255)
                  ttACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCt                                                                                                                                 <  2:840070/148‑1 (MQ=255)
                   tACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTc                                                                                                                                >  2:3367107/1‑148 (MQ=255)
                    aCCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCg                                                                                                                               >  1:2520139/1‑148 (MQ=255)
                     ccGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGc                                                                                                                              >  2:1902651/1‑148 (MQ=255)
                     ccGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGc                                                                                                                              >  2:2287667/1‑148 (MQ=255)
                      cGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGcc                                                                                                                             <  2:3428343/148‑1 (MQ=255)
                       gCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGccc                                                                                                                            <  2:3087652/148‑1 (MQ=255)
                        cTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAATCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCct                                                                                                                           <  2:2023676/148‑1 (MQ=255)
                        cTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCct                                                                                                                           <  2:3070281/148‑1 (MQ=255)
                          cAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCctct                                                                                                                         >  1:20577/1‑148 (MQ=255)
                          cAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCctct                                                                                                                         >  1:2853801/1‑148 (MQ=255)
                              ccTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCca                                                                                                                     >  1:287541/1‑148 (MQ=255)
                              ccTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCca                                                                                                                     >  2:3179438/1‑148 (MQ=255)
                               cTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcac                                                                                                                    >  1:1991749/1‑148 (MQ=255)
                               cTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCcac                                                                                                                    >  2:2625160/1‑148 (MQ=255)
                                ttCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCcaca                                                                                                                   >  1:3002872/1‑148 (MQ=255)
                                     gCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGc                                                                                                              <  2:656685/148‑1 (MQ=255)
                                        aTAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctg                                                                                                           >  1:3387011/1‑148 (MQ=255)
                                          aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCTGATCGCCGAAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAATCACAGTCAGCGGAGACTCTTGCTAGCCCACTGCCACACACGCctgct                                                                                                         >  2:3500672/1‑148 (MQ=255)
                                          aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCGGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgct                                                                                                         <  2:876257/148‑1 (MQ=255)
                                          aaCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgct                                                                                                         <  2:882701/148‑1 (MQ=255)
                                           aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                                                                                                        <  2:3405639/148‑1 (MQ=255)
                                           aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                                                                                                        >  1:1646340/1‑148 (MQ=255)
                                            cGCACAATTTATCTTCGCGGAATCGAGAATAAAGCGGATCGACGCAAACGGGACTTCATTGACGAACTTAAGGATCTCAGCCAGCGTCGTTCTGGAAATCACATAAAGCGGAGACTAATGCTACCCCTCAGCCACAAACACcagctcg                                                                                                       >  1:2396335/1‑146 (MQ=255)
                                            cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTgg                                                                                                       >  1:3537485/1‑148 (MQ=255)
                                            cGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTgg                                                                                                       >  1:500605/1‑148 (MQ=255)
                                             gCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTggg                                                                                                      <  1:3319980/148‑1 (MQ=255)
                                                 aaTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaaa                                                                                                  <  2:429854/148‑1 (MQ=255)
                                                   tttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAaca                                                                                                >  2:1830099/1‑148 (MQ=255)
                                                   tttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAaca                                                                                                <  1:441801/148‑1 (MQ=255)
                                                    ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac                                                                                               >  1:2277327/1‑148 (MQ=255)
                                                    ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac                                                                                               <  2:3341276/148‑1 (MQ=255)
                                                    ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac                                                                                               <  2:2469231/148‑1 (MQ=255)
                                                     tAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacacc                                                                                              >  2:557033/1‑148 (MQ=255)
                                                       gCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCAGCCACACACGCCTGCTGGGTAAacaccac                                                                                            >  1:54367/1‑148 (MQ=255)
                                                          tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacacc                                                                                         >  2:107693/1‑148 (MQ=255)
                                                           cgcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAcaccacacca                                                                                        <  1:1108512/148‑1 (MQ=255)
                                                            gcgGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                                                       <  1:2429491/148‑1 (MQ=255)
                                                               gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt                                                                                    >  2:2783897/1‑148 (MQ=255)
                                                                   cGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGt                                                                                >  2:253919/1‑148 (MQ=255)
                                                                     agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct                                                                              <  2:1682869/148‑1 (MQ=255)
                                                                     agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct                                                                              <  2:3447249/148‑1 (MQ=255)
                                                                        aTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa                                                                           <  1:1910988/148‑1 (MQ=255)
                                                                         tAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGat                                                                          >  2:964169/1‑148 (MQ=255)
                                                                                gATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcaca                                                                   <  1:77467/148‑1 (MQ=255)
                                                                                 aTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACaa                                                                  <  1:2625160/148‑1 (MQ=255)
                                                                                         aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                                                          <  2:312728/148‑1 (MQ=255)
                                                                                         aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                                                          <  2:36481/148‑1 (MQ=255)
                                                                                           aCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATg                                                        >  1:224606/1‑148 (MQ=255)
                                                                                            cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGc                                                       >  1:1309643/1‑148 (MQ=255)
                                                                                            cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGc                                                       >  1:2545314/1‑148 (MQ=255)
                                                                                              ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGccc                                                     >  1:2732528/1‑148 (MQ=255)
                                                                                               gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccg                                                    >  2:2909257/1‑148 (MQ=255)
                                                                                                  ttCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccgccg                                                 <  2:181632/148‑1 (MQ=255)
                                                                                                  ttCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccgccg                                                 <  2:2461227/148‑1 (MQ=255)
                                                                                                     aTTGACGAACTTCAGGATCTCAGCCAGCGTAGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacg                                              >  2:270950/1‑148 (MQ=255)
                                                                                                      ttGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga                                             <  1:348396/148‑1 (MQ=255)
                                                                                                      ttGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga                                             <  1:348355/148‑1 (MQ=255)
                                                                                                        gACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATg                                           <  2:2782918/148‑1 (MQ=255)
                                                                                                          cGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGt                                         >  2:2980729/1‑148 (MQ=255)
                                                                                                            aaCTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGa                                       >  1:3205786/1‑148 (MQ=255)
                                                                                                                tCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGCCGATGGTGacaca                                   >  2:2168065/1‑148 (MQ=255)
                                                                                                                   ggATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGc                                >  2:3122847/1‑148 (MQ=255)
                                                                                                                    gATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGcc                               >  2:1182084/1‑148 (MQ=255)
                                                                                                                    gATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGcc                               >  1:1511426/1‑148 (MQ=255)
                                                                                                                        tcAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACt                           <  1:1138611/148‑1 (MQ=255)
                                                                                                                         cagccagcGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACtt                          >  2:237198/1‑148 (MQ=255)
                                                                                                                           gccagcGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTtct                        >  1:2927299/1‑148 (MQ=255)
                                                                                                                                cgtcgtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCg                   <  1:343994/148‑1 (MQ=255)
                                                                                                                                cgtcgtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCg                   <  1:1788436/148‑1 (MQ=255)
                                                                                                                                 gtcgtTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGt                  <  2:332443/148‑1 (MQ=255)
                                                                                                                                  tcgtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGtt                 >  2:2501039/1‑148 (MQ=255)
                                                                                                                                   cgtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTc                >  2:1444135/1‑148 (MQ=255)
                                                                                                                                   cgtTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTc                >  1:982100/1‑148 (MQ=255)
                                                                                                                                   cgtTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTc                >  2:1451561/1‑148 (MQ=255)
                                                                                                                                   cgtTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTc                >  2:3490024/1‑148 (MQ=255)
                                                                                                                                   cgtTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTc                >  2:2665489/1‑148 (MQ=255)
                                                                                                                                      tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAg             >  2:1652633/1‑148 (MQ=255)
                                                                                                                                       cTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGa            <  1:312683/148‑1 (MQ=255)
                                                                                                                                         ggAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACt          >  1:2125519/1‑148 (MQ=255)
                                                                                                                                         ggAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACt          <  2:282374/148‑1 (MQ=255)
                                                                                                                                          gAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACtt         >  2:1222557/1‑148 (MQ=255)
                                                                                                                                          gAAAGCACAGTCAGCGGAGACACTTGCTCGCCCTCTGCCACACACCCCAGCTGGGTAAACACCACACCAACGTGCGTCTCGATATGCACAAAGTTGGTATGCCCCCCCACCATGGTGACACACCCCCACAACTCACCGTTCCAGAcat         >  2:1222330/1‑146 (MQ=255)
                                                                                                                                           aaaGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACttt        <  2:774188/148‑1 (MQ=255)
                                                                                                                                             aGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTgg      >  2:2619154/1‑148 (MQ=255)
                                                                                                                                             aGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTgg      >  1:292818/1‑148 (MQ=255)
                                                                                                                                               cACCGTCAGCGGAGACTCTTGCTCGCCCTCGGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGgcg    <  1:1249287/148‑1 (MQ=255)
                                                                                                                                                 ccGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGcgcg  <  1:2069539/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCG  >  CP009273/3249328‑3249620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGA  >  CP009273/3249326‑3249623
                                                                                                                                                    |                                                                                                                                                     
CGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAG                                                                                                                                                      <  SRR19973066.2518672/150‑1 (MQ=60)
CGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAG                                                                                                                                                      <  SRR19973066.2518700/150‑1 (MQ=60)
    AATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAG                                                                                                                                                  <  SRR19973066.3351637/150‑1 (MQ=60)
    AATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAG                                                                                                                                                  <  SRR19973066.1573988/150‑1 (MQ=60)
      TATGCAGCCCCCAATTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAATCGGATCGCCGCATACGGGACTTCATTGACTAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCG                                                                                                                                                <  SRR19973066.750728/150‑1 (MQ=60)
            GCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACT                                                                                                                                          <  SRR19973066.2129694/150‑1 (MQ=60)
               CCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTT                                                                                                                                       >  SRR19973066.2375026/1‑150 (MQ=60)
               CCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTT                                                                                                                                       >  SRR19973066.2374918/1‑150 (MQ=60)
               CCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTT                                                                                                                                       >  SRR19973066.2512403/1‑150 (MQ=60)
                CCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTG                                                                                                                                      <  SRR19973066.518665/150‑1 (MQ=60)
                  ACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCT                                                                                                                                    <  SRR19973066.1614467/150‑1 (MQ=60)
                   CTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTC                                                                                                                                   <  SRR19973066.2949/150‑1 (MQ=60)
                   CTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTC                                                                                                                                   <  SRR19973066.4581/150‑1 (MQ=60)
                    TTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG                                                                                                                                  >  SRR19973066.2537277/1‑150 (MQ=60)
                    TTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCG                                                                                                                                  <  SRR19973066.1144659/150‑1 (MQ=60)
                      ACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCC                                                                                                                                >  SRR19973066.2425470/1‑150 (MQ=60)
                         GCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTC                                                                                                                             <  SRR19973066.2334436/150‑1 (MQ=60)
                          CTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCT                                                                                                                            >  SRR19973066.20934/1‑150 (MQ=60)
                          CTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCT                                                                                                                            >  SRR19973066.20884/1‑150 (MQ=60)
                          CTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCT                                                                                                                            >  SRR19973066.2874027/1‑150 (MQ=60)
                          CTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCT                                                                                                                            >  SRR19973066.2872979/1‑150 (MQ=60)
                          CTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCT                                                                                                                            >  SRR19973066.2874258/1‑150 (MQ=60)
                            CAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGC                                                                                                                          >  SRR19973066.2487326/1‑150 (MQ=60)
                              AACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                                                                                        >  SRR19973066.289609/1‑150 (MQ=60)
                              AACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCA                                                                                                                        >  SRR19973066.947531/1‑150 (MQ=60)
                               ACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCAC                                                                                                                       >  SRR19973066.2005766/1‑150 (MQ=60)
                                CCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACA                                                                                                                      >  SRR19973066.3022836/1‑150 (MQ=60)
                                CCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACA                                                                                                                      >  SRR19973066.3023098/1‑150 (MQ=60)
                                CCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACA                                                                                                                      >  SRR19973066.2968109/1‑150 (MQ=60)
                                        CGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTG                                                                                                              >  SRR19973066.1630625/1‑150 (MQ=60)
                                        CGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTG                                                                                                              >  SRR19973066.3409613/1‑150 (MQ=60)
                                           TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                                                                                           >  SRR19973066.1658329/1‑150 (MQ=60)
                                           TAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTG                                                                                                           >  SRR19973066.3355805/1‑150 (MQ=60)
                                            AACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                                                                                          >  SRR19973066.2511954/1‑150 (MQ=60)
                                            AACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                                                                                          >  SRR19973066.712339/1‑150 (MQ=60)
                                            AACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                                                                                          <  SRR19973066.2664221/150‑1 (MQ=60)
                                            AACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                                                                                          >  SRR19973066.3561000/1‑150 (MQ=60)
                                            AACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGG                                                                                                          >  SRR19973066.504328/1‑150 (MQ=60)
                                             ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGG                                                                                                         <  SRR19973066.1318779/150‑1 (MQ=60)
                                              CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTTGGT                                                                                                        >  SRR19973066.769556/1‑150 (MQ=60)
                                              CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGT                                                                                                        <  SRR19973066.407512/150‑1 (MQ=60)
                                               GCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTA                                                                                                       <  SRR19973066.3342030/150‑1 (MQ=60)
                                               GCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTA                                                                                                       <  SRR19973066.3341899/150‑1 (MQ=60)
                                                    ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACAC                                                                                                  >  SRR19973066.2292969/1‑150 (MQ=60)
                                                    ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACAC                                                                                                  >  SRR19973066.2853512/1‑150 (MQ=60)
                                                    ATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACAC                                                                                                  >  SRR19973066.2292645/1‑150 (MQ=60)
                                                     TTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACC                                                                                                 <  SRR19973066.445216/150‑1 (MQ=60)
                                                       TAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCAGCCACACACGCCTGCTGGGTAAACACCAC                                                                                               >  SRR19973066.55054/1‑150 (MQ=60)
                                                             CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                                                                                         <  SRR19973066.1116535/150‑1 (MQ=60)
                                                             CGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                                                                                         <  SRR19973066.2019872/150‑1 (MQ=60)
                                                              GCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                                                                                        <  SRR19973066.2446016/150‑1 (MQ=60)
                                                                  AATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCG                                                                                    <  SRR19973066.3465565/150‑1 (MQ=60)
                                                                          ATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA                                                                            <  SRR19973066.1924595/150‑1 (MQ=60)
                                                                              AGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCAGGGTAAAAACCACACCCACCTGCGTCTCGTTATGCA                                                                        >  SRR19973066.2867855/1‑150 (MQ=60)
                                                                                  GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAT                                                                    <  SRR19973066.78373/150‑1 (MQ=60)
                                                                                  GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAT                                                                    <  SRR19973066.962267/150‑1 (MQ=60)
                                                                                   ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATG                                                                   <  SRR19973066.2642860/150‑1 (MQ=60)
                                                                                   ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATG                                                                   <  SRR19973066.2641048/150‑1 (MQ=60)
                                                                                           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATG                                                           >  SRR19973066.226421/1‑150 (MQ=60)
                                                                                            AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGC                                                          >  SRR19973066.1319147/1‑150 (MQ=60)
                                                                                            AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGC                                                          >  SRR19973066.1319197/1‑150 (MQ=60)
                                                                                            AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGC                                                          >  SRR19973066.2562609/1‑150 (MQ=60)
                                                                                              CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCC                                                        >  SRR19973066.2750911/1‑150 (MQ=60)
                                                                                              CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCC                                                        >  SRR19973066.1660800/1‑150 (MQ=60)
                                                                                                       ATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGAT                                               >  SRR19973066.2513584/1‑150 (MQ=60)
                                                                                                       ATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTATGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGACCGCCGACGAT                                               >  SRR19973066.3544857/1‑150 (MQ=60)
                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                              <  SRR19973066.1843165/150‑1 (MQ=60)
                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                              <  SRR19973066.351188/150‑1 (MQ=60)
                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                              <  SRR19973066.351113/150‑1 (MQ=60)
                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                              <  SRR19973066.351072/150‑1 (MQ=60)
                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                              <  SRR19973066.351073/150‑1 (MQ=60)
                                                                                                            CGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGA                                          >  SRR19973066.3227055/1‑150 (MQ=60)
                                                                                                                    AGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCC                                  >  SRR19973066.1522008/1‑150 (MQ=60)
                                                                                                                          TCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTC                            <  SRR19973066.1146758/150‑1 (MQ=60)
                                                                                                                           CAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCT                           >  SRR19973066.2946795/1‑150 (MQ=60)
                                                                                                                                AGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCG                      >  SRR19973066.1956836/1‑150 (MQ=60)
                                                                                                                                  CGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTT                    <  SRR19973066.1801183/150‑1 (MQ=60)
                                                                                                                                  CGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTT                    <  SRR19973066.346658/150‑1 (MQ=60)
                                                                                                                                  CGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTT                    <  SRR19973066.344415/150‑1 (MQ=60)
                                                                                                                                  CGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTT                    >  SRR19973066.1566327/1‑150 (MQ=60)
                                                                                                                                  CGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTT                    >  SRR19973066.3346504/1‑150 (MQ=60)
                                                                                                                                   GTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTC                   >  SRR19973066.989345/1‑150 (MQ=60)
                                                                                                                                     CGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCA                 <  SRR19973066.2210022/150‑1 (MQ=60)
                                                                                                                                         CTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACT             >  SRR19973066.2140236/1‑150 (MQ=60)
                                                                                                                                         CTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACT             <  SRR19973066.314986/150‑1 (MQ=60)
                                                                                                                                            GAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTG          <  SRR19973066.3211321/150‑1 (MQ=60)
                                                                                                                                             AAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGG         >  SRR19973066.294911/1‑150 (MQ=60)
                                                                                                                                             AAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGG         >  SRR19973066.294909/1‑150 (MQ=60)
                                                                                                                                              AAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGC        <  SRR19973066.2544964/150‑1 (MQ=60)
                                                                                                                                                 CACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTCTGGCGCG     <  SRR19973066.1258225/150‑1 (MQ=60)
                                                                                                                                                 CACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCGGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCG     <  SRR19973066.3332837/150‑1 (MQ=60)
                                                                                                                                                 CACCGTCAGCGGAGACTCTTGCTCGCCCTCGGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCG     <  SRR19973066.1258255/150‑1 (MQ=60)
                                                                                                                                                   CCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTG   <  SRR19973066.2083866/150‑1 (MQ=60)
                                                                                                                                                   CCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTG   <  SRR19973066.2084335/150‑1 (MQ=60)
                                                                                                                                                    AGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGA  >  SRR19973066.773038/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGA  >  CP009273/3249326‑3249623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑