Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,249,474	C→A	93.7%	T179T (ACG→ACT)	yhaM ←	putative L‑serine dehydratase alpha chain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,249,474	0	C	A	93.7%	647.6 / 31.2	231	T179T (ACG→ACT)	yhaM	putative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand): ref base C (8/7); new base A (104/111); total (112/119)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

CAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCG  >  CP009273/3249329‑3249618

cAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGt                                                                                                                                                >  1:394429/1‑148 (MQ=255)

cAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGt                                                                                                                                                >  1:3465677/1‑148 (MQ=255)

cAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGt                                                                                                                                                <  1:640822/148‑1 (MQ=255)

  atatGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCa                                                                                                                                              >  2:2462606/1‑148 (MQ=255)

    atGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGc                                                                                                                                            >  2:2655112/1‑148 (MQ=255)

    atGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGc                                                                                                                                            >  2:3150475/1‑148 (MQ=255)

    atGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGc                                                                                                                                            <  1:1894028/148‑1 (MQ=255)

      gCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCgg                                                                                                                                          <  2:3466579/148‑1 (MQ=255)

      gCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCgg                                                                                                                                          >  2:1732639/1‑148 (MQ=255)

      gCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGAgg                                                                                                                                          <  1:2595656/148‑1 (MQ=255)

        aGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGgag                                                                                                                                        <  2:297543/148‑1 (MQ=255)

         gCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGgaga                                                                                                                                       <  1:1674434/148‑1 (MQ=255)

         gCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGgaga                                                                                                                                       >  2:2952519/1‑148 (MQ=255)

            cccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGActc                                                                                                                                    >  2:427913/1‑148 (MQ=255)

            cccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGActc                                                                                                                                    <  1:479310/148‑1 (MQ=255)

            cccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGActc                                                                                                                                    <  1:3184871/148‑1 (MQ=255)

             ccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGActct                                                                                                                                   <  2:1398207/148‑1 (MQ=255)

             ccACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGActct                                                                                                                                   <  1:2342286/148‑1 (MQ=255)

               aCTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTg                                                                                                                                 <  1:159793/148‑1 (MQ=255)

                cTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGc                                                                                                                                >  1:747318/1‑148 (MQ=255)

                  tACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTc                                                                                                                              >  1:3091832/1‑148 (MQ=255)

                    ccGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGc                                                                                                                            >  1:2515579/1‑148 (MQ=255)

                     cGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGcc                                                                                                                           <  1:100311/148‑1 (MQ=255)

                       cTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCct                                                                                                                         <  1:2302945/148‑1 (MQ=255)

                            aCCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGcc                                                                                                                    >  1:481127/1‑148 (MQ=255)

                            aCCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGcc                                                                                                                    >  1:96574/1‑148 (MQ=255)

                               ttCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCcaca                                                                                                                 >  2:724093/1‑148 (MQ=255)

                                tCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCcacac                                                                                                                <  2:1500153/148‑1 (MQ=255)

                                   tGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACg                                                                                                             >  2:1755234/1‑148 (MQ=255)

                                          aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                                                                                                      <  1:3291831/148‑1 (MQ=255)

                                          aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                                                                                                      <  2:1903891/148‑1 (MQ=255)

                                          aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                                                                                                      <  1:913865/148‑1 (MQ=255)

                                          aCGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCctgctg                                                                                                      >  2:901681/1‑148 (MQ=255)

                                               caATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaa                                                                                                 <  2:1773741/148‑1 (MQ=255)

                                                aaTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAATCACAGACAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaaa                                                                                                >  1:2814936/1‑148 (MQ=255)

                                                aaTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaaa                                                                                                >  2:514352/1‑148 (MQ=255)

                                                aaTTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTaaa                                                                                                >  1:1343608/1‑148 (MQ=255)

                                                   ttAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacac                                                                                             >  2:609874/1‑148 (MQ=255)

                                                      gCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccac                                                                                          <  1:1844777/148‑1 (MQ=255)

                                                       cTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccaca                                                                                         >  2:1484407/1‑148 (MQ=255)

                                                         tCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAacaccacacc                                                                                       >  2:2303543/1‑148 (MQ=255)

                                                             ggAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                                                   <  2:384272/148‑1 (MQ=255)

                                                              gAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt                                                                                  >  1:247281/1‑148 (MQ=255)

                                                               aaTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTg                                                                                 >  1:2670823/1‑148 (MQ=255)

                                                               aaTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCATGCTGGGTAAACACCACACCATCGTg                                                                                 <  2:757728/148‑1 (MQ=255)

                                                                aTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGc                                                                                >  1:2109415/1‑148 (MQ=255)

                                                                  cGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGt                                                                              >  2:2119149/1‑148 (MQ=255)

                                                                   gagaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc                                                                             <  1:2853207/148‑1 (MQ=255)

                                                                    agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct                                                                            >  1:3481181/1‑148 (MQ=255)

                                                                    agaATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtct                                                                            >  2:1017360/1‑148 (MQ=255)

                                                                      aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                                                          >  2:2165278/1‑148 (MQ=255)

                                                                      aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                                                          >  2:877416/1‑148 (MQ=255)

                                                                      aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                                                          <  1:2746013/148‑1 (MQ=255)

                                                                      aaTAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                                                          <  2:1382607/148‑1 (MQ=255)

                                                                        tAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGat                                                                        >  2:1647217/1‑148 (MQ=255)

                                                                        tAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGat                                                                        <  2:2801142/148‑1 (MQ=255)

                                                                        tAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGat                                                                        >  2:299667/1‑148 (MQ=255)

                                                                               gATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcaca                                                                 >  2:2566033/1‑148 (MQ=255)

                                                                               gATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcaca                                                                 <  1:1602615/148‑1 (MQ=255)

                                                                                aTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATCTGCACaa                                                                >  2:3443951/1‑148 (MQ=255)

                                                                                      gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTgg                                                          <  1:31663/148‑1 (MQ=255)

                                                                                        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                                                        <  1:2643095/148‑1 (MQ=255)

                                                                                          aCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATg                                                      >  1:2733518/1‑148 (MQ=255)

                                                                                              gACTACATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccg                                                  <  2:3104712/148‑1 (MQ=255)

                                                                                                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcga                                              >  2:1310936/1‑148 (MQ=255)

                                                                                                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcga                                              <  2:1438474/148‑1 (MQ=255)

                                                                                                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAGATGCACAATGTTGGTCTGCCCGCcga                                              >  2:3471904/1‑148 (MQ=255)

                                                                                                     ttGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga                                           <  1:1102737/148‑1 (MQ=255)

                                                                                                     ttGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga                                           <  1:1264708/148‑1 (MQ=255)

                                                                                                     ttGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga                                           >  2:3315407/1‑148 (MQ=255)

                                                                                                     ttGACGAACTTCAGGATCTCAGCCAGCGTCGTTCAGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCACTGCCACACACGCCTGCTGGGTAAACACCACACCAACGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga                                           >  1:3349810/1‑148 (MQ=255)

                                                                                                      tGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGAt                                          <  2:3203339/148‑1 (MQ=255)

                                                                                                        aCGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg                                        >  2:2919500/1‑148 (MQ=255)

                                                                                                        aCGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg                                        >  1:1688218/1‑148 (MQ=255)

                                                                                                        aCGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg                                        >  2:1084974/1‑148 (MQ=255)

                                                                                                        aCGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg                                        <  1:1049157/148‑1 (MQ=255)

                                                                                                           aaCTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGa                                     <  1:670924/148‑1 (MQ=255)

                                                                                                              ttCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacac                                  <  1:1212570/148‑1 (MQ=255)

                                                                                                                 aGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACg                               <  1:2952519/148‑1 (MQ=255)

                                                                                                                 aGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACg                               >  1:1790811/1‑148 (MQ=255)

                                                                                                                 aGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACg                               >  1:10589/1‑148 (MQ=255)

                                                                                                                  ggATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGc                              <  2:1143662/148‑1 (MQ=255)

                                                                                                                    aTCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGccc                            >  1:2477829/1‑148 (MQ=255)

                                                                                                                      ctcAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCAc                          <  1:514352/148‑1 (MQ=255)

                                                                                                                         agccagcGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTtc                       <  1:1501787/148‑1 (MQ=255)

                                                                                                                           ccagcGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTtctc                     >  1:3242887/1‑148 (MQ=255)

                                                                                                                           ccagcGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTtctc                     <  1:1609582/148‑1 (MQ=255)

                                                                                                                           ccagcGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTtctc                     <  1:1488931/148‑1 (MQ=255)

                                                                                                                                  cgtTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTc              <  2:2396592/148‑1 (MQ=255)

                                                                                                                                  cgtTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTc              >  1:306776/1‑148 (MQ=255)

                                                                                                                                   gtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTcc             <  1:3150475/148‑1 (MQ=255)

                                                                                                                                     tCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAg           <  1:3008556/148‑1 (MQ=255)

                                                                                                                                     tCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAg           >  1:1808105/1‑148 (MQ=255)

                                                                                                                                        ggAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACt        <  1:453800/148‑1 (MQ=255)

                                                                                                                                        ggAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACt        <  1:2874632/148‑1 (MQ=255)

                                                                                                                                           aaGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTg     <  1:2172039/148‑1 (MQ=255)

                                                                                                                                            aGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTgg    >  2:2711456/1‑148 (MQ=255)

                                                                                                                                            aGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTgg    <  1:1182012/148‑1 (MQ=255)

                                                                                                                                            aGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTgg    >  2:961969/1‑148 (MQ=255)

                                                                                                                                              cacaGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGgcg  >  2:399607/1‑148 (MQ=255)

CAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCG  >  CP009273/3249329‑3249618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTG  >  CP009273/3249326‑3249622

CGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCG                                                                                                                                                     <  SRR19973065.2027893/150‑1 (MQ=60)

CGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCG                                                                                                                                                     <  SRR19973065.2027846/150‑1 (MQ=60)

 GCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGT                                                                                                                                                    >  SRR19973065.397187/1‑150 (MQ=60)

 GCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGT                                                                                                                                                    >  SRR19973065.3486783/1‑150 (MQ=60)

 GCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGT                                                                                                                                                    >  SRR19973065.3486699/1‑150 (MQ=60)

 GCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGT                                                                                                                                                    >  SRR19973065.509264/1‑150 (MQ=60)

  CCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTC                                                                                                                                                   <  SRR19973065.1696396/150‑1 (MQ=60)

   CAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAANGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCA                                                                                                                                                  >  SRR19973065.1794022/1‑150 (MQ=60)

   CAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCA                                                                                                                                                  <  SRR19973065.3524722/150‑1 (MQ=60)

   CAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCA                                                                                                                                                  >  SRR19973065.2498736/1‑150 (MQ=60)

   CAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCA                                                                                                                                                  <  SRR19973065.645024/150‑1 (MQ=60)

      TATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCG                                                                                                                                               <  SRR19973065.1384775/150‑1 (MQ=60)

       ATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGG                                                                                                                                              <  SRR19973065.1906473/150‑1 (MQ=60)

       ATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGG                                                                                                                                              <  SRR19973065.1906701/150‑1 (MQ=60)

         GCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGAGGAG                                                                                                                                            <  SRR19973065.2611791/150‑1 (MQ=60)

            GCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACT                                                                                                                                         <  SRR19973065.1685654/150‑1 (MQ=60)

            GCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACT                                                                                                                                         <  SRR19973065.1685636/150‑1 (MQ=60)

               CCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTT                                                                                                                                      <  SRR19973065.3352688/150‑1 (MQ=60)

               CCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTT                                                                                                                                      <  SRR19973065.483292/150‑1 (MQ=60)

               CCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTT                                                                                                                                      <  SRR19973065.482577/150‑1 (MQ=60)

               CCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTT                                                                                                                                      <  SRR19973065.3204102/150‑1 (MQ=60)

                CCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTG                                                                                                                                     <  SRR19973065.2356186/150‑1 (MQ=60)

                CCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTG                                                                                                                                     <  SRR19973065.2356906/150‑1 (MQ=60)

                 CACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGC                                                                                                                                    >  SRR19973065.752316/1‑150 (MQ=60)

                  ACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCT                                                                                                                                   <  SRR19973065.161106/150‑1 (MQ=60)

                  ACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCT                                                                                                                                   <  SRR19973065.161112/150‑1 (MQ=60)

                   CTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTC                                                                                                                                  >  SRR19973065.3110712/1‑150 (MQ=60)

                    TTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAATCGGATCGCCGCAAACGGGACTTCATTGAAGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCG                                                                                                                                 >  SRR19973065.3195287/1‑150 (MQ=60)

                    TTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCG                                                                                                                                 >  SRR19973065.3416215/1‑150 (MQ=60)

                     TACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGC                                                                                                                                >  SRR19973065.2531383/1‑150 (MQ=60)

                        CGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCT                                                                                                                             >  SRR19973065.2582707/1‑150 (MQ=60)

                        CGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCT                                                                                                                             >  SRR19973065.2582563/1‑150 (MQ=60)

                        CGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCT                                                                                                                             <  SRR19973065.101230/150‑1 (MQ=60)

                          CTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCT                                                                                                                           <  SRR19973065.2317429/150‑1 (MQ=60)

                           TCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGACTCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGACAGCGTCGTTCTGGAAATCACAGTCAGCGGAGACTCTTGCTCGCCCTCTG                                                                                                                          >  SRR19973065.2887438/1‑150 (MQ=60)

                             AAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCC                                                                                                                        >  SRR19973065.97474/1‑150 (MQ=60)

                             AAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCC                                                                                                                        >  SRR19973065.484402/1‑150 (MQ=60)

                                       GCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCT                                                                                                              <  SRR19973065.398435/150‑1 (MQ=60)

                                             ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGG                                                                                                        <  SRR19973065.2953923/150‑1 (MQ=60)

                                             ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGG                                                                                                        <  SRR19973065.919836/150‑1 (MQ=60)

                                             ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGG                                                                                                        <  SRR19973065.919825/150‑1 (MQ=60)

                                             ACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGG                                                                                                        <  SRR19973065.3311869/150‑1 (MQ=60)

                                              CGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGT                                                                                                       >  SRR19973065.1151047/1‑150 (MQ=60)

                                                CACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAA                                                                                                     >  SRR19973065.1812526/1‑150 (MQ=60)

                                                 ACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAATCACAGACAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                                                                                                    >  SRR19973065.2832333/1‑150 (MQ=60)

                                                 ACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAA                                                                                                    >  SRR19973065.1352495/1‑150 (MQ=60)

                                                  CAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAAC                                                                                                   >  SRR19973065.514743/1‑150 (MQ=60)

                                                   AATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACA                                                                                                  >  SRR19973065.2523114/1‑150 (MQ=60)

                                                         GCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                                                                                            <  SRR19973065.3000699/150‑1 (MQ=60)

                                                         GCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                                                                                            <  SRR19973065.2707755/150‑1 (MQ=60)

                                                         GCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                                                                                            <  SRR19973065.1856940/150‑1 (MQ=60)

                                                         GCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACAC                                                                                            <  SRR19973065.1856941/150‑1 (MQ=60)

                                                              GCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCG                                                                                       >  SRR19973065.786371/1‑150 (MQ=60)

                                                               CGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGT                                                                                      >  SRR19973065.249027/1‑150 (MQ=60)

                                                                GGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG                                                                                     >  SRR19973065.2687369/1‑150 (MQ=60)

                                                                 GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC                                                                                    >  SRR19973065.2123004/1‑150 (MQ=60)

                                                                 GAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC                                                                                    >  SRR19973065.2122982/1‑150 (MQ=60)

                                                                     CGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCT                                                                                <  SRR19973065.2328761/150‑1 (MQ=60)

                                                                     CGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCT                                                                                >  SRR19973065.3502364/1‑150 (MQ=60)

                                                                      GAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCNC                                                                               <  SRR19973065.2870747/150‑1 (MQ=60)

                                                                       AGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG                                                                              >  SRR19973065.1071844/1‑150 (MQ=60)

                                                                         AATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAT                                                                            >  SRR19973065.1508153/1‑150 (MQ=60)

                                                                         AATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAT                                                                            <  SRR19973065.2763099/150‑1 (MQ=60)

                                                                               GCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCAC                                                                      <  SRR19973065.1060551/150‑1 (MQ=60)

                                                                               GCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCAC                                                                      <  SRR19973065.403253/150‑1 (MQ=60)

                                                                                CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACA                                                                     <  SRR19973065.2845862/150‑1 (MQ=60)

                                                                                CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACA                                                                     <  SRR19973065.2845804/150‑1 (MQ=60)

                                                                                 GGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAC                                                                    >  SRR19973065.1664777/1‑150 (MQ=60)

                                                                                  GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAT                                                                   <  SRR19973065.1613331/150‑1 (MQ=60)

                                                                                  GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAT                                                                   <  SRR19973065.1613237/150‑1 (MQ=60)

                                                                                   ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATG                                                                  >  SRR19973065.256183/1‑150 (MQ=60)

                                                                                   ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATG                                                                  >  SRR19973065.1153501/1‑150 (MQ=60)

                                                                                     CGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTT                                                                <  SRR19973065.61/150‑1 (MQ=60)

                                                                                       CCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGG                                                              <  SRR19973065.669625/150‑1 (MQ=60)

                                                                                         GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTA                                                            >  SRR19973065.2640183/1‑150 (MQ=60)

                                                                                         GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTA                                                            <  SRR19973065.32059/150‑1 (MQ=60)

                                                                                         GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTA                                                            <  SRR19973065.3225501/150‑1 (MQ=60)

                                                                                          CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAT                                                           <  SRR19973065.3180656/150‑1 (MQ=60)

                                                                                           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATG                                                          >  SRR19973065.1021707/1‑150 (MQ=60)

                                                                                           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATG                                                          >  SRR19973065.2750552/1‑150 (MQ=60)

                                                                                           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATG                                                          >  SRR19973065.2750522/1‑150 (MQ=60)

                                                                                           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATG                                                          >  SRR19973065.2750497/1‑150 (MQ=60)

                                                                                           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATG                                                          <  SRR19973065.2659404/150‑1 (MQ=60)

                                                                                           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTATTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATG                                                          <  SRR19973065.2659425/150‑1 (MQ=60)

                                                                                               GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCG                                                      >  SRR19973065.3112100/1‑150 (MQ=60)

                                                                                                  ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCG                                                   >  SRR19973065.772331/1‑150 (MQ=60)

                                                                                                      CATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCAGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCACTGCCACACACGCCTGCTGGGTAAACACCACACCAACGTGCGTCTCGATATGCACAATGTTGGTATTCCCGCCGACGA                                               >  SRR19973065.3370281/1‑150 (MQ=60)

                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                             <  SRR19973065.3030847/150‑1 (MQ=60)

                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                             <  SRR19973065.1273045/150‑1 (MQ=60)

                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                             <  SRR19973065.1109979/150‑1 (MQ=60)

                                                                                                        TTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG                                             <  SRR19973065.1273755/150‑1 (MQ=60)

                                                                                                         TGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGG                                            >  SRR19973065.1699493/1‑150 (MQ=60)

                                                                                                           ACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTG                                          <  SRR19973065.1056104/150‑1 (MQ=60)

                                                                                                             GAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGAC                                        <  SRR19973065.1514978/150‑1 (MQ=60)

                                                                                                             GAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGAC                                        >  SRR19973065.3087069/1‑150 (MQ=60)

                                                                                                             GAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGAC                                        <  SRR19973065.3266149/150‑1 (MQ=60)

                                                                                                             GAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGAC                                        >  SRR19973065.1288633/1‑150 (MQ=60)

                                                                                                              AACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACA                                       >  SRR19973065.123420/1‑150 (MQ=60)

                                                                                                              AACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACA                                       <  SRR19973065.675396/150‑1 (MQ=60)

                                                                                                                 TTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC                                    <  SRR19973065.1220404/150‑1 (MQ=60)

                                                                                                                 TTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC                                    <  SRR19973065.1220395/150‑1 (MQ=60)

                                                                                                                 TTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC                                    <  SRR19973065.3132540/150‑1 (MQ=60)

                                                                                                                  TCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACG                                   >  SRR19973065.1802552/1‑150 (MQ=60)

                                                                                                                  TCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACG                                   >  SRR19973065.10752/1‑150 (MQ=60)

                                                                                                                    AGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCC                                 <  SRR19973065.2970524/150‑1 (MQ=60)

                                                                                                                    AGGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCC                                 <  SRR19973065.2970510/150‑1 (MQ=60)

                                                                                                                     GGGTCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACATTGTTGGTATGCCCGCCGACGATGGTGCCACACGCCC                                >  SRR19973065.721194/1‑150 (MQ=60)

                                                                                                                     GGATCTCAGCCAGCGTCGTTCTGGAACGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC                                >  SRR19973065.2370889/1‑150 (MQ=60)

                                                                                                                     GGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC                                >  SRR19973065.2493429/1‑150 (MQ=60)

                                                                                                                     GGATCTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC                                >  SRR19973065.2493434/1‑150 (MQ=60)

                                                                                                                         CTCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTT                            <  SRR19973065.517754/150‑1 (MQ=60)

                                                                                                                          TCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTC                           >  SRR19973065.3229817/1‑150 (MQ=60)

                                                                                                                          TCAGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTC                           <  SRR19973065.2811281/150‑1 (MQ=60)

                                                                                                                            AGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTC                         >  SRR19973065.3262437/1‑150 (MQ=60)

                                                                                                                            AGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTC                         >  SRR19973065.3498215/1‑150 (MQ=60)

                                                                                                                            AGCCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTC                         <  SRR19973065.1511492/150‑1 (MQ=60)

                                                                                                                              CCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCAC                       <  SRR19973065.1620374/150‑1 (MQ=60)

                                                                                                                              CCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCAC                       <  SRR19973065.1620265/150‑1 (MQ=60)

                                                                                                                              CCAGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCAC                       <  SRR19973065.1498464/150‑1 (MQ=60)

                                                                                                                                AGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCG                     >  SRR19973065.2029405/1‑150 (MQ=60)

                                                                                                                                AGCGTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACCCCTGCTGGGTAAACACCACACCAACGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACATCTCACCG                     >  SRR19973065.1621976/1‑150 (MQ=60)

                                                                                                                                   GTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTC                  >  SRR19973065.993084/1‑150 (MQ=60)

                                                                                                                                   GTCGTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTC                  >  SRR19973065.308815/1‑150 (MQ=60)

                                                                                                                                      GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAG               <  SRR19973065.3169597/150‑1 (MQ=60)

                                                                                                                                      GTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAG               >  SRR19973065.1819996/1‑150 (MQ=60)

                                                                                                                                      GTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAG               >  SRR19973065.1820001/1‑150 (MQ=60)

                                                                                                                                      GTTCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAG               >  SRR19973065.1820007/1‑150 (MQ=60)

                                                                                                                                        TCTGGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGAC             <  SRR19973065.3026980/150‑1 (MQ=60)

                                                                                                                                           GGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTT          <  SRR19973065.457663/150‑1 (MQ=60)

                                                                                                                                           GGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTT          <  SRR19973065.456941/150‑1 (MQ=60)

                                                                                                                                           GGAAAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTT          <  SRR19973065.2892243/150‑1 (MQ=60)

                                                                                                                                              AAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGC       <  SRR19973065.2185977/150‑1 (MQ=60)

                                                                                                                                              AAGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGC       <  SRR19973065.2186183/150‑1 (MQ=60)

                                                                                                                                               AGCACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCG      <  SRR19973065.1189497/150‑1 (MQ=60)

                                                                                                                                                 CACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCG    >  SRR19973065.2018187/1‑150 (MQ=60)

                                                                                                                                                 CACAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCG    >  SRR19973065.232602/1‑150 (MQ=60)

                                                                                                                                                   CAGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTG  <  SRR19973065.3215784/150‑1 (MQ=60)

CGCCAATATGCAGCCCCCACTTACCGCTCAAACCTTCCTGCGATAACGCACAATTTAGCTTCGCGGAATCGAGAATAAAGCGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTG  >  CP009273/3249326‑3249622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`