Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,699,862 T→A 44.4% L119* (TTG→TAG)  ydgK → DUF2569 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,699,8620TA44.4% 122.9 / inf 257L119* (TTG→TAG) ydgKDUF2569 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (87/56);  new base A (65/49);  total (152/105)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

GTATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATT  >  CP009273/1699713‑1700011
                                                                                                                                                     |                                                                                                                                                     
gTATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGtt                                                                                                                                                       <  1:2755128/150‑1 (MQ=255)
  aTTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGc                                                                                                                                                     >  2:2055700/1‑150 (MQ=255)
  aTTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGc                                                                                                                                                     >  1:3049535/1‑150 (MQ=255)
    tACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAATCATTCGCTTTTTAAACCGTGGAAGATGGCATATCCGTTCGCCAGTTGCTg                                                                                                                                                   >  1:3321302/1‑150 (MQ=255)
    tACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTg                                                                                                                                                   <  1:1012938/150‑1 (MQ=255)
         acTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTAc                                                                                                                                              <  1:3756163/150‑1 (MQ=255)
          cTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACt                                                                                                                                             >  2:1073391/1‑150 (MQ=255)
            gtgGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACttt                                                                                                                                           <  1:179653/150‑1 (MQ=255)
                ttGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTttgttg                                                                                                                                       >  2:3553265/1‑150 (MQ=255)
                ttGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTttgttg                                                                                                                                       <  1:1482770/150‑1 (MQ=255)
                    ccATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCaa                                                                                                                                   >  1:351181/1‑150 (MQ=255)
                        cGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGc                                                                                                                               >  2:2180995/1‑150 (MQ=255)
                         gCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACAGCa                                                                                                                              >  2:2854994/1‑150 (MQ=255)
                           tttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATATGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGAATTCGCTTTTTAACCCGTGGAAGATGGCATAGACGTTCGCCAGTAGCTGTTTACTTTTTTTGCAACCGCAta                                                                                                                            >  1:3642843/1‑149 (MQ=255)
                            ttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTg                                                                                                                           >  1:4089069/1‑150 (MQ=255)
                              ctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGAt                                                                                                                         <  1:54500/150‑1 (MQ=255)
                                  aaaCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTc                                                                                                                     <  2:1782287/150‑1 (MQ=255)
                                   aaCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcc                                                                                                                    <  1:211261/150‑1 (MQ=255)
                                   aaCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcc                                                                                                                    >  2:2098170/1‑150 (MQ=255)
                                    aCGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTccc                                                                                                                   <  2:3005437/150‑1 (MQ=255)
                                     cGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcccc                                                                                                                  >  1:1557232/1‑150 (MQ=255)
                                      gTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCt                                                                                                                 <  1:3238056/150‑1 (MQ=255)
                                      gTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCt                                                                                                                 <  1:47801/150‑1 (MQ=255)
                                      gTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCt                                                                                                                 <  1:2926533/150‑1 (MQ=255)
                                        cGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAt                                                                                                               >  2:3790666/1‑150 (MQ=255)
                                         ggCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCACCCGCATTGATCGTCCCCTAtt                                                                                                              >  2:2961984/1‑150 (MQ=255)
                                         ggCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGAGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAtt                                                                                                              <  1:836193/150‑1 (MQ=255)
                                          gCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAttt                                                                                                             <  2:51662/150‑1 (MQ=255)
                                           cGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTc                                                                                                            <  2:1464017/150‑1 (MQ=255)
                                              tgtgTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCaaa                                                                                                         >  1:2125632/1‑150 (MQ=255)
                                               gtgtTCCTAAACACTATCTTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCAGTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCAAGTAGCTGATTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAAc                                                                                                        <  2:4325338/150‑1 (MQ=255)
                                               gtgtTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAAc                                                                                                        <  1:664816/150‑1 (MQ=255)
                                               gtgtTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAAc                                                                                                        >  1:717912/1‑150 (MQ=255)
                                                 gtTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGc                                                                                                      >  2:1862816/1‑150 (MQ=255)
                                                            cTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGt                                                                                           >  2:2815902/1‑150 (MQ=255)
                                                              atatTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCAGTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTaa                                                                                         >  2:3370179/1‑150 (MQ=255)
                                                                attatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTaaaa                                                                                       >  1:3652155/1‑150 (MQ=255)
                                                                 ttatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAg                                                                                      >  2:897234/1‑150 (MQ=255)
                                                                  tatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGc                                                                                     >  2:1646868/1‑150 (MQ=255)
                                                                  tatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGc                                                                                     >  2:1837426/1‑150 (MQ=255)
                                                                   atCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCg                                                                                    >  2:1222158/1‑150 (MQ=255)
                                                                   atCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCg                                                                                    >  2:3572701/1‑150 (MQ=255)
                                                                   atCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCg                                                                                    >  2:25355/1‑150 (MQ=255)
                                                                    tCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGa                                                                                   <  1:2782140/150‑1 (MQ=255)
                                                                      tGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACg                                                                                 >  1:1105511/1‑150 (MQ=255)
                                                                      tGGCTGCTTAGTCCCGTATTGCTGGAATTAAAAGAATTCGATTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCAAGTAGCTGTTTACTTTGTTGGCAACCGAATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACg                                                                                 <  1:2675863/150‑1 (MQ=255)
                                                                        gctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGtt                                                                               >  1:1932974/1‑150 (MQ=255)
                                                                        gcggctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCTTTCGCAAGTTGCTGTTTAATTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGtt                                                                               <  2:2409607/147‑1 (MQ=255)
                                                                           gctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtgt                                                                            >  2:621162/1‑150 (MQ=255)
                                                                              tATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGaa                                                                         >  2:3360668/1‑150 (MQ=255)
                                                                                 ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATcc                                                                      <  2:717912/150‑1 (MQ=255)
                                                                                 ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATcc                                                                      <  1:2354503/150‑1 (MQ=255)
                                                                                      tATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                                 >  2:3076442/1‑150 (MQ=255)
                                                                                       aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaata                                                                <  1:3864653/150‑1 (MQ=255)
                                                                                        ttGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaataa                                                               >  2:1327997/1‑150 (MQ=255)
                                                                                         tGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAAc                                                              <  1:4159713/150‑1 (MQ=255)
                                                                                         tGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAAc                                                              <  1:2661087/150‑1 (MQ=255)
                                                                                          gCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAAcc                                                             <  1:2499349/150‑1 (MQ=255)
                                                                                           cTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCt                                                            <  1:1348935/150‑1 (MQ=255)
                                                                                             ggCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTa                                                          >  2:2636135/1‑150 (MQ=255)
                                                                                                 gTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGt                                                      <  1:1628543/150‑1 (MQ=255)
                                                                                                   aaaaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTa                                                    >  2:345081/1‑150 (MQ=255)
                                                                                                   aaaaGCATTCGCTTTTTCACACGTGGAAGAAGGCATAGCCGTTAGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTAACAGTTa                                                    >  2:3641856/1‑150 (MQ=255)
                                                                                                    aaaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTaa                                                   >  1:4272963/1‑150 (MQ=255)
                                                                                                     atgCATTCGCTTTTTGACCCGTGGATGATGGCTTAGCAGTTCGCCTGTTGCTGTTTACTTTGTTGGCTACCGAATTGATCGTCAACTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                                  <  1:1731217/148‑1 (MQ=255)
                                                                                                      aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAcc                                                 >  1:360789/1‑150 (MQ=255)
                                                                                                      aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAcc                                                 <  2:2699195/150‑1 (MQ=255)
                                                                                                      aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAcc                                                 >  2:1516427/1‑150 (MQ=255)
                                                                                                      aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAcc                                                 >  2:3423975/1‑150 (MQ=255)
                                                                                                          ttCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgtt                                             <  2:4272963/150‑1 (MQ=255)
                                                                                                          ttCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgtt                                             <  1:343736/150‑1 (MQ=255)
                                                                                                           tCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgttg                                            >  2:312724/1‑150 (MQ=255)
                                                                                                            cGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgttgt                                           >  1:2024392/1‑150 (MQ=255)
                                                                                                              cTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGGTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCg                                         >  1:2709119/1‑150 (MQ=255)
                                                                                                              cTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCg                                         >  2:3997424/1‑150 (MQ=255)
                                                                                                              cTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCg                                         <  2:4315244/150‑1 (MQ=255)
                                                                                                                        cGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTgg                               <  1:1389767/150‑1 (MQ=255)
                                                                                                                        cGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTgg                               <  1:2025544/150‑1 (MQ=255)
                                                                                                                        cGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTgg                               >  2:2019700/1‑150 (MQ=255)
                                                                                                                        cGTGGAAGAGGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTgg                               <  2:2916471/150‑1 (MQ=255)
                                                                                                                            gAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTa                           <  1:2416780/150‑1 (MQ=255)
                                                                                                                             aaGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                          >  1:3798372/1‑150 (MQ=255)
                                                                                                                             aaGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                          >  1:3075758/1‑150 (MQ=255)
                                                                                                                              aGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAAc                         >  1:800170/1‑150 (MQ=255)
                                                                                                                               gATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACg                        <  2:4168399/150‑1 (MQ=255)
                                                                                                                                 tGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                      >  2:1006421/1‑150 (MQ=255)
                                                                                                                                 tGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                      >  2:3062788/1‑150 (MQ=255)
                                                                                                                                   gCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGata                    <  1:3685755/150‑1 (MQ=255)
                                                                                                                                    cATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataa                   >  1:3849392/1‑150 (MQ=255)
                                                                                                                                     aTAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataat                  >  1:3357207/1‑150 (MQ=255)
                                                                                                                                      tAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataata                 <  1:4341816/150‑1 (MQ=255)
                                                                                                                                      tAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataata                 <  1:3521268/150‑1 (MQ=255)
                                                                                                                                       aGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAg                >  1:4129735/1‑150 (MQ=255)
                                                                                                                                         ccGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGATCTGGATTAAAGGATAATAggc              >  2:3734356/1‑150 (MQ=255)
                                                                                                                                           gTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcgg            <  1:3301820/150‑1 (MQ=255)
                                                                                                                                               gCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCttt        <  2:1402146/150‑1 (MQ=255)
                                                                                                                                               gCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCttt        <  2:3445397/150‑1 (MQ=255)
                                                                                                                                                ccAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCtttt       >  2:3658968/1‑150 (MQ=255)
                                                                                                                                                 cAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCttttt      <  2:3669378/150‑1 (MQ=255)
                                                                                                                                                 cAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCttttt      >  1:629499/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTTATT  >  CP009273/1699713‑1700011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A