Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,699,862 T→A 100% L119* (TTG→TAG)  ydgK → DUF2569 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,699,8620TA99.5% 665.4 / ‑4.6 206L119* (TTG→TAG) ydgKDUF2569 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (111/94);  total (111/95)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

TACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTT  >  CP009273/1699717‑1700006
                                                                                                                                                 |                                                                                                                                                
tACACACTGTGGTTGACCCTCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGc                                                                                                                                                >  2:1933320/1‑148 (MQ=255)
tACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGc                                                                                                                                                >  2:1933586/1‑148 (MQ=255)
  cacacTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTg                                                                                                                                              <  2:782540/148‑1 (MQ=255)
          ggTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACttt                                                                                                                                      >  2:1872654/1‑148 (MQ=255)
            ttGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgt                                                                                                                                    <  1:1016171/148‑1 (MQ=255)
               aCCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTgg                                                                                                                                 <  2:3109410/148‑1 (MQ=255)
               aCCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTgg                                                                                                                                 >  2:1001634/1‑148 (MQ=255)
                ccATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGc                                                                                                                                >  2:476989/1‑148 (MQ=255)
                 cATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCa                                                                                                                               >  2:2770287/1‑148 (MQ=255)
                   tCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAAc                                                                                                                             <  1:3395545/148‑1 (MQ=255)
                      ctttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGc                                                                                                                          >  2:2067547/1‑148 (MQ=255)
                        ttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAt                                                                                                                        >  1:1062987/1‑148 (MQ=255)
                        ttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAt                                                                                                                        >  1:2814679/1‑148 (MQ=255)
                          ctttAAACGTCGGCGTTGTGTTCCTAAACCCTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAtag                                                                                                                      >  1:1279365/1‑146 (MQ=255)
                          ctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTg                                                                                                                      <  1:1861423/148‑1 (MQ=255)
                          ctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTg                                                                                                                      <  1:2657725/148‑1 (MQ=255)
                          ctttAAACGTCGGCGTTGCGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTg                                                                                                                      <  1:902359/148‑1 (MQ=255)
                           tttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGa                                                                                                                     >  2:56763/1‑148 (MQ=255)
                            ttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTTGCAACCGCATTGAt                                                                                                                    >  1:2104100/1‑148 (MQ=255)
                            ttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGAt                                                                                                                    <  2:2211857/148‑1 (MQ=255)
                            ttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGAt                                                                                                                    <  2:1664910/148‑1 (MQ=255)
                                  gTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTccc                                                                                                              <  1:1934448/148‑1 (MQ=255)
                                  gTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTccc                                                                                                              <  1:3486317/148‑1 (MQ=255)
                                    cGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCt                                                                                                            <  1:3496756/148‑1 (MQ=255)
                                        gTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAttt                                                                                                        <  1:2272626/148‑1 (MQ=255)
                                          tgtgTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCa                                                                                                      >  1:1197411/1‑148 (MQ=255)
                                          tgtgTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCa                                                                                                      >  1:464001/1‑148 (MQ=255)
                                               tCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGc                                                                                                 >  2:1410810/1‑148 (MQ=255)
                                               tCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGc                                                                                                 >  1:1121986/1‑148 (MQ=255)
                                                ccTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCt                                                                                                <  1:3190753/148‑1 (MQ=255)
                                                ccTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCt                                                                                                >  2:108122/1‑148 (MQ=255)
                                                 cTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtc                                                                                               <  1:1794734/148‑1 (MQ=255)
                                                  tAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcg                                                                                              >  1:1248176/1‑148 (MQ=255)
                                                   aaaCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgt                                                                                             >  2:1134599/1‑148 (MQ=255)
                                                   aaaCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgt                                                                                             <  1:2351684/148‑1 (MQ=255)
                                                     acacTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgtcg                                                                                           >  1:1729829/1‑148 (MQ=255)
                                                       acTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTcgcg                                                                                         >  1:982526/1‑148 (MQ=255)
                                                        cTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCgg                                                                                        >  2:1306630/1‑148 (MQ=255)
                                                         tataTTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCggg                                                                                       >  1:735205/1‑148 (MQ=255)
                                                         tataTTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCACCTATTTCAAACGCTCGTCGCggg                                                                                       >  2:3292824/1‑148 (MQ=255)
                                                            attatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTaa                                                                                    >  2:2219664/1‑148 (MQ=255)
                                                             ttatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTaaa                                                                                   <  2:4897/148‑1 (MQ=255)
                                                              tatCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTaaaa                                                                                  >  1:389201/1‑148 (MQ=255)
                                                                   ggCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGAc                                                                             >  1:2507961/1‑148 (MQ=255)
                                                                     ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGTTGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGt                                                                           <  1:86194/148‑1 (MQ=255)
                                                                     ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGt                                                                           <  1:2923269/148‑1 (MQ=255)
                                                                     ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGt                                                                           <  2:457561/148‑1 (MQ=255)
                                                                        ctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtg                                                                        <  1:3365306/148‑1 (MQ=255)
                                                                           aTTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGa                                                                     <  1:3357954/148‑1 (MQ=255)
                                                                           aTTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGa                                                                     <  1:583790/148‑1 (MQ=255)
                                                                             ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                   >  2:748689/1‑148 (MQ=255)
                                                                             ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                   >  2:1977146/1‑148 (MQ=255)
                                                                             ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                   >  1:2107047/1‑148 (MQ=255)
                                                                             ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                   >  2:2689232/1‑148 (MQ=255)
                                                                                  ttttGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATGGATCGTCCCCTATTTAAAACGCCCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGta                                                              <  1:1620010/146‑1 (MQ=255)
                                                                                  tATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGta                                                              >  1:3248697/1‑148 (MQ=255)
                                                                                  tATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGta                                                              >  1:3362317/1‑148 (MQ=255)
                                                                                  tATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGta                                                              >  1:2576293/1‑148 (MQ=255)
                                                                                  tATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGta                                                              >  2:1059925/1‑148 (MQ=255)
                                                                                   aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaa                                                             >  1:3377351/1‑148 (MQ=255)
                                                                                   aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaa                                                             >  2:3403004/1‑148 (MQ=255)
                                                                                    ttGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                            >  1:2144479/1‑148 (MQ=255)
                                                                                    ttGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                            >  1:3311594/1‑148 (MQ=255)
                                                                                    ttGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaat                                                            >  1:1654742/1‑148 (MQ=255)
                                                                                           cAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTa                                                     >  1:2396878/1‑148 (MQ=255)
                                                                                              tAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAg                                                  <  2:3231522/148‑1 (MQ=255)
                                                                                               aaaaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGt                                                 <  1:1933576/148‑1 (MQ=255)
                                                                                                aaaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGtt                                                >  1:87661/1‑148 (MQ=255)
                                                                                                 aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTa                                               <  1:1471584/148‑1 (MQ=255)
                                                                                                 aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTa                                               <  1:1067266/148‑1 (MQ=255)
                                                                                                  aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTaa                                              <  2:354577/148‑1 (MQ=255)
                                                                                                  aGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTaa                                              >  2:1799241/1‑148 (MQ=255)
                                                                                                    cATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAcc                                            <  2:2910766/148‑1 (MQ=255)
                                                                                                           tttttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCCTTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTc                                     >  1:2668390/1‑148 (MQ=255)
                                                                                                           tttttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTc                                     >  1:2109885/1‑148 (MQ=255)
                                                                                                                  cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGct                              <  2:2434582/148‑1 (MQ=255)
                                                                                                                  cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGct                              >  2:3098228/1‑148 (MQ=255)
                                                                                                                  cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGct                              <  2:1581769/148‑1 (MQ=255)
                                                                                                                   ccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctc                             >  1:2695717/1‑148 (MQ=255)
                                                                                                                      tGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTgg                          <  2:2378956/148‑1 (MQ=255)
                                                                                                                      tGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTGTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTgg                          <  2:1006303/148‑1 (MQ=255)
                                                                                                                       ggAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGa                         <  2:770738/148‑1 (MQ=255)
                                                                                                                           gATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                     <  2:2303428/148‑1 (MQ=255)
                                                                                                                            aTGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAAc                    >  2:719102/1‑148 (MQ=255)
                                                                                                                              ggCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACgg                  <  1:1915137/148‑1 (MQ=255)
                                                                                                                               gCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                 >  1:537255/1‑148 (MQ=255)
                                                                                                                               gCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                 >  2:2157/1‑148 (MQ=255)
                                                                                                                               gCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                 >  1:3474778/1‑148 (MQ=255)
                                                                                                                                cATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGat                <  1:383837/148‑1 (MQ=255)
                                                                                                                                 aTAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGata               >  1:2980298/1‑148 (MQ=255)
                                                                                                                                   aGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataat             <  1:1386060/148‑1 (MQ=255)
                                                                                                                                    gCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataata            <  1:2448420/148‑1 (MQ=255)
                                                                                                                                     ccGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAg           <  2:2814679/148‑1 (MQ=255)
                                                                                                                                     ccGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAg           >  2:1182971/1‑148 (MQ=255)
                                                                                                                                     ccGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAg           <  1:768557/148‑1 (MQ=255)
                                                                                                                                       gTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggc         <  1:476989/148‑1 (MQ=255)
                                                                                                                                         tCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcgg       <  1:3142893/148‑1 (MQ=255)
                                                                                                                                           gCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCt     >  2:2464169/1‑148 (MQ=255)
                                                                                                                                              aGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCtttt  <  1:271928/148‑1 (MQ=255)
                                                                                                                                              aGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCtttt  >  2:951023/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTT  >  CP009273/1699717‑1700006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTT  >  CP009273/1699714‑1700008
                                                                                                                                                    |                                                                                                                                                  
TATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAG                                                                                                                                                   <  SRR19973065.900698/150‑1 (MQ=60)
              GTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTT                                                                                                                                     <  SRR19973065.1071067/150‑1 (MQ=60)
              GTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTT                                                                                                                                     <  SRR19973065.3167694/150‑1 (MQ=60)
               TTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTG                                                                                                                                    <  SRR19973065.1022902/150‑1 (MQ=60)
                 GACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGC                                                                                                                                  >  SRR19973065.1477229/1‑150 (MQ=60)
                    CATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAAC                                                                                                                               >  SRR19973065.2182871/1‑150 (MQ=60)
                     ATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACC                                                                                                                              >  SRR19973065.877020/1‑150 (MQ=60)
                     ATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACC                                                                                                                              >  SRR19973065.1176369/1‑150 (MQ=60)
                      TCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCG                                                                                                                             <  SRR19973065.3416221/150‑1 (MQ=60)
                      TCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCG                                                                                                                             <  SRR19973065.3416307/150‑1 (MQ=60)
                         CTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAT                                                                                                                          >  SRR19973065.474548/1‑150 (MQ=60)
                         CTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAT                                                                                                                          >  SRR19973065.2832075/1‑150 (MQ=60)
                         CTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAT                                                                                                                          >  SRR19973065.1070031/1‑150 (MQ=60)
                          TTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATT                                                                                                                         <  SRR19973065.1616346/150‑1 (MQ=60)
                           TTCTTTAAACGTCGGCGTTGTGTTCCTAAACCCTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATAG                                                                                                                        >  SRR19973065.1287810/1‑150 (MQ=60)
                           TTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTG                                                                                                                        <  SRR19973065.2962814/150‑1 (MQ=60)
                           TTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTG                                                                                                                        >  SRR19973065.3421568/1‑150 (MQ=60)
                            TCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCCTTGA                                                                                                                       >  SRR19973065.2396042/1‑150 (MQ=60)
                             CTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTTGCAACCGCATTGAT                                                                                                                      >  SRR19973065.2117624/1‑150 (MQ=60)
                             CTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGAT                                                                                                                      <  SRR19973065.2674152/150‑1 (MQ=60)
                             CTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGAT                                                                                                                      >  SRR19973065.2117674/1‑150 (MQ=60)
                             CTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGAT                                                                                                                      <  SRR19973065.1873677/150‑1 (MQ=60)
                             CTTTAAACGTCGGCGTTGCGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGAT                                                                                                                      <  SRR19973065.908158/150‑1 (MQ=60)
                                 AAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTC                                                                                                                  >  SRR19973065.2698813/1‑150 (MQ=60)
                                     GTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCT                                                                                                              <  SRR19973065.3507520/150‑1 (MQ=60)
                                     GTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCT                                                                                                              <  SRR19973065.1947112/150‑1 (MQ=60)
                                       CGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAT                                                                                                            <  SRR19973065.3518005/150‑1 (MQ=60)
                                        GGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATT                                                                                                           <  SRR19973065.3515516/150‑1 (MQ=60)
                                           GTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTTGCAACCGCATTGATCGTCCCCTATTTCA                                                                                                        >  SRR19973065.1250385/1‑150 (MQ=60)
                                           GTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCA                                                                                                        >  SRR19973065.1205046/1‑150 (MQ=60)
                                           GTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCA                                                                                                        >  SRR19973065.467205/1‑150 (MQ=60)
                                           GTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCA                                                                                                        <  SRR19973065.2286974/150‑1 (MQ=60)
                                           GTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCA                                                                                                        >  SRR19973065.467019/1‑150 (MQ=60)
                                               TGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTAGCTTTTTAACCCGTGGAAGATGGCATATCCGTTCGCCAGTAGATGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACG                                                                                                    >  SRR19973065.1676/1‑150 (MQ=60)
                                                GTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGC                                                                                                   >  SRR19973065.1129296/1‑150 (MQ=60)
                                                 TTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCTCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCACCTATTTAAAACGCT                                                                                                  >  SRR19973065.3250942/1‑150 (MQ=60)
                                                   CCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCG                                                                                                <  SRR19973065.3209999/150‑1 (MQ=60)
                                                   CCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCCTTGATCGTCCCCTATTTCAAACGCTCG                                                                                                >  SRR19973065.1256398/1‑150 (MQ=60)
                                                   CCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCG                                                                                                >  SRR19973065.2086109/1‑150 (MQ=60)
                                                    CTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGT                                                                                               <  SRR19973065.1806513/150‑1 (MQ=60)
                                                      AAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCG                                                                                             >  SRR19973065.1741288/1‑150 (MQ=60)
                                                      AAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCG                                                                                             <  SRR19973065.2366323/150‑1 (MQ=60)
                                                        ACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCG                                                                                           >  SRR19973065.2754242/1‑150 (MQ=60)
                                                        ACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCG                                                                                           >  SRR19973065.989048/1‑150 (MQ=60)
                                                          ACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGG                                                                                         >  SRR19973065.740119/1‑150 (MQ=60)
                                                            TATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTA                                                                                       <  SRR19973065.1259446/150‑1 (MQ=60)
                                                             ATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAA                                                                                      >  SRR19973065.3352094/1‑150 (MQ=60)
                                                               ATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAA                                                                                    >  SRR19973065.391912/1‑150 (MQ=60)
                                                               ATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAA                                                                                    >  SRR19973065.391917/1‑150 (MQ=60)
                                                                    CTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGAC                                                                               >  SRR19973065.2523720/1‑150 (MQ=60)
                                                                    CTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGAC                                                                               >  SRR19973065.2075483/1‑150 (MQ=60)
                                                                     TGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACG                                                                              >  SRR19973065.1874274/1‑150 (MQ=60)
                                                                        CTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGTTGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTT                                                                           <  SRR19973065.87032/150‑1 (MQ=60)
                                                                        CTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTT                                                                           <  SRR19973065.2941031/150‑1 (MQ=60)
                                                                          GCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGT                                                                         <  SRR19973065.3026972/150‑1 (MQ=60)
                                                                           CTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTG                                                                        <  SRR19973065.3385884/150‑1 (MQ=60)
                                                                           CTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTG                                                                        <  SRR19973065.2627666/150‑1 (MQ=60)
                                                                              ATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAT                                                                     <  SRR19973065.3378482/150‑1 (MQ=60)
                                                                              ATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAT                                                                     >  SRR19973065.2120594/1‑150 (MQ=60)
                                                                              ATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAT                                                                     <  SRR19973065.587424/150‑1 (MQ=60)
                                                                              ATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAT                                                                     >  SRR19973065.2120634/1‑150 (MQ=60)
                                                                              ATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAT                                                                     <  SRR19973065.2467566/150‑1 (MQ=60)
                                                                              ATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGAATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAT                                                                     <  SRR19973065.3381467/150‑1 (MQ=60)
                                                                                TTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCC                                                                   <  SRR19973065.707810/150‑1 (MQ=60)
                                                                                   CGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTA                                                                >  SRR19973065.3268322/1‑150 (MQ=60)
                                                                                   CGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTA                                                                >  SRR19973065.3268908/1‑150 (MQ=60)
                                                                                   CGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTA                                                                >  SRR19973065.481846/1‑150 (MQ=60)
                                                                                   CGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTA                                                                >  SRR19973065.3382874/1‑150 (MQ=60)
                                                                                   CGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTA                                                                >  SRR19973065.3382927/1‑150 (MQ=60)
                                                                                   CGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTA                                                                >  SRR19973065.2592361/1‑150 (MQ=60)
                                                                                    GTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAA                                                               >  SRR19973065.3398003/1‑150 (MQ=60)
                                                                                     TTTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGAATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATGGATCGTCCCCTATTTAAAACGCCCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAAT                                                              <  SRR19973065.1630873/150‑1 (MQ=60)
                                                                                     TATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAAT                                                              >  SRR19973065.1665835/1‑150 (MQ=60)
                                                                                     TATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAAT                                                              >  SRR19973065.3331778/1‑150 (MQ=60)
                                                                                     TATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAAT                                                              >  SRR19973065.2158271/1‑150 (MQ=60)
                                                                                       TTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAA                                                            <  SRR19973065.38202/150‑1 (MQ=60)
                                                                                           TGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTT                                                        <  SRR19973065.481704/150‑1 (MQ=60)
                                                                                            GGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTA                                                       >  SRR19973065.2411951/1‑150 (MQ=60)
                                                                                            GGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTA                                                       >  SRR19973065.2411974/1‑150 (MQ=60)
                                                                                                 TAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTCCTTTGTTGGCAACCGCATTGATCGTCACCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTT                                                  >  SRR19973065.88641/1‑150 (MQ=60)
                                                                                                 TAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTT                                                  >  SRR19973065.781086/1‑150 (MQ=60)
                                                                                                 TAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTT                                                  >  SRR19973065.88509/1‑150 (MQ=60)
                                                                                                  AAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATCGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTA                                                 <  SRR19973065.1945974/150‑1 (MQ=60)
                                                                                                  AAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTA                                                 <  SRR19973065.1946232/150‑1 (MQ=60)
                                                                                                  AAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTA                                                 <  SRR19973065.972714/150‑1 (MQ=60)
                                                                                                  AAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTA                                                 <  SRR19973065.1946242/150‑1 (MQ=60)
                                                                                                  AAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTA                                                 <  SRR19973065.2734116/150‑1 (MQ=60)
                                                                                                   AAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAA                                                <  SRR19973065.1869726/150‑1 (MQ=60)
                                                                                                    AAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAC                                               <  SRR19973065.1481011/150‑1 (MQ=60)
                                                                                                    AAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAC                                               <  SRR19973065.1074332/150‑1 (MQ=60)
                                                                                                    AAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAC                                               <  SRR19973065.2563813/150‑1 (MQ=60)
                                                                                                      GCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCT                                             >  SRR19973065.1317861/1‑150 (MQ=60)
                                                                                                            GCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCCTTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTC                                       >  SRR19973065.2684914/1‑150 (MQ=60)
                                                                                                            GCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTC                                       >  SRR19973065.2123458/1‑150 (MQ=60)
                                                                                                                    ACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTC                               >  SRR19973065.2712457/1‑150 (MQ=60)
                                                                                                                         TGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAT                          >  SRR19973065.1084589/1‑150 (MQ=60)
                                                                                                                         TGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAT                          <  SRR19973065.1645601/150‑1 (MQ=60)
                                                                                                                         TGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAT                          <  SRR19973065.3394987/150‑1 (MQ=60)
                                                                                                                          GGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATT                         >  SRR19973065.284921/1‑150 (MQ=60)
                                                                                                                              GATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACG                     <  SRR19973065.2683894/150‑1 (MQ=60)
                                                                                                                               ATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGG                    >  SRR19973065.3504311/1‑150 (MQ=60)
                                                                                                                               ATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGG                    >  SRR19973065.1757754/1‑150 (MQ=60)
                                                                                                                                TGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGA                   >  SRR19973065.540753/1‑150 (MQ=60)
                                                                                                                                TGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGA                   >  SRR19973065.1025305/1‑150 (MQ=60)
                                                                                                                                TGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGA                   >  SRR19973065.3495917/1‑150 (MQ=60)
                                                                                                                                 GGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGAT                  <  SRR19973065.1927703/150‑1 (MQ=60)
                                                                                                                                 GGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGAT                  <  SRR19973065.57368/150‑1 (MQ=60)
                                                                                                                                  GCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATA                 >  SRR19973065.2998528/1‑150 (MQ=60)
                                                                                                                                   CATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAA                <  SRR19973065.386518/150‑1 (MQ=60)
                                                                                                                                     TAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATA              <  SRR19973065.2341843/150‑1 (MQ=60)
                                                                                                                                      AGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAG             <  SRR19973065.1395169/150‑1 (MQ=60)
                                                                                                                                       GCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGG            <  SRR19973065.2463848/150‑1 (MQ=60)
                                                                                                                                        CCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGC           <  SRR19973065.3147225/150‑1 (MQ=60)
                                                                                                                                        CCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGC           <  SRR19973065.772354/150‑1 (MQ=60)
                                                                                                                                        CCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGC           <  SRR19973065.773665/150‑1 (MQ=60)
                                                                                                                                          GTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGG         <  SRR19973065.480245/150‑1 (MQ=60)
                                                                                                                                            TCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCT       <  SRR19973065.3161989/150‑1 (MQ=60)
                                                                                                                                               CCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTT    >  SRR19973065.21547/1‑150 (MQ=60)
                                                                                                                                                 AGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTT  <  SRR19973065.273763/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
TATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTT  >  CP009273/1699714‑1700008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑