Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,694,843 G→T 44.5% I82I (ATC→ATA)  pdxJ ← pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,694,8430GT44.5% 205.7 / inf 276I82I (ATC→ATA) pdxJpyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (85/68);  new base T (63/60);  total (148/128)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAT  >  CP009273/2694694‑2694992
                                                                                                                                                     |                                                                                                                                                     
agagAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCg                                                                                                                                                       >  1:1237181/1‑150 (MQ=255)
agagAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCg                                                                                                                                                       <  1:500972/150‑1 (MQ=255)
   gaAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATc                                                                                                                                                    >  1:2789484/1‑150 (MQ=255)
       ccTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCa                                                                                                                                                >  1:3856746/1‑150 (MQ=255)
         tGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAACTGTGGCTTCGTCTCAACGGCTATCGCCAGc                                                                                                                                              >  2:3026508/1‑150 (MQ=255)
            aTCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtc                                                                                                                                           <  1:3022165/150‑1 (MQ=255)
             tCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtct                                                                                                                                          <  1:1596020/150‑1 (MQ=255)
             tCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtct                                                                                                                                          <  2:2722345/150‑1 (MQ=255)
             tCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtct                                                                                                                                          <  1:2233043/150‑1 (MQ=255)
               ccGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATctct                                                                                                                                        <  2:2409240/150‑1 (MQ=255)
                 ggCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTc                                                                                                                                      >  1:835454/1‑150 (MQ=255)
                 ggCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTc                                                                                                                                      >  2:2851423/1‑150 (MQ=255)
                 ggCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTc                                                                                                                                      <  1:33946/150‑1 (MQ=255)
                  gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCg                                                                                                                                     <  2:3651398/150‑1 (MQ=255)
                  gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCg                                                                                                                                     <  1:4164803/150‑1 (MQ=255)
                  gCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCg                                                                                                                                     <  1:2496856/150‑1 (MQ=255)
                    aTCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGt                                                                                                                                   >  1:3227884/1‑150 (MQ=255)
                      cTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCa                                                                                                                                 <  2:2440706/150‑1 (MQ=255)
                           aGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGcc                                                                                                                            >  2:1135897/1‑150 (MQ=255)
                            gACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCa                                                                                                                           >  1:3139915/1‑150 (MQ=255)
                             aCGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAt                                                                                                                          >  2:1382892/1‑150 (MQ=255)
                               gTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAtct                                                                                                                        >  2:591438/1‑150 (MQ=255)
                                tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAtctc                                                                                                                       <  2:4305624/150‑1 (MQ=255)
                                   gCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAg                                                                                                                    <  1:3748728/150‑1 (MQ=255)
                                       gCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTc                                                                                                                >  1:63723/1‑150 (MQ=255)
                                        cATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCa                                                                                                               >  2:943661/1‑150 (MQ=255)
                                         aTCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCAt                                                                                                              >  2:3394359/1‑150 (MQ=255)
                                           cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATGTGTTCGGTCACCGCCATCTCCAGATTCATGc                                                                                                            <  2:3871446/150‑1 (MQ=255)
                                           cgcgCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGc                                                                                                            >  2:3606776/1‑150 (MQ=255)
                                            gcgcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCg                                                                                                           <  2:2670910/150‑1 (MQ=255)
                                             cgcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCgg                                                                                                          >  1:1992870/1‑150 (MQ=255)
                                               cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGt                                                                                                        >  1:2387666/1‑150 (MQ=255)
                                               cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGt                                                                                                        >  1:2858302/1‑150 (MQ=255)
                                               cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGt                                                                                                        >  2:2162969/1‑150 (MQ=255)
                                                 ttttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCTGTCACCGCCAACTCCAGATTCATGCGGGTAt                                                                                                      >  2:2259923/1‑150 (MQ=255)
                                                  tttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATc                                                                                                     <  2:1679514/150‑1 (MQ=255)
                                                     gTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAg                                                                                                  >  1:3979264/1‑150 (MQ=255)
                                                     gTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAg                                                                                                  >  2:4153216/1‑150 (MQ=255)
                                                       cACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCg                                                                                                <  1:1641519/150‑1 (MQ=255)
                                                        aCGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGt                                                                                               <  2:1048573/150‑1 (MQ=255)
                                                          gCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCt                                                                                             >  1:2538916/1‑150 (MQ=255)
                                                          gCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCt                                                                                             <  2:1434070/150‑1 (MQ=255)
                                                           cTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTg                                                                                            <  2:2370923/150‑1 (MQ=255)
                                                               ccTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGc                                                                                        >  1:3543033/1‑150 (MQ=255)
                                                                cTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCa                                                                                       <  2:3005904/150‑1 (MQ=255)
                                                                cTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCa                                                                                       >  1:3612430/1‑150 (MQ=255)
                                                                 tGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAg                                                                                      >  1:3721909/1‑150 (MQ=255)
                                                                  gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAgg                                                                                     <  2:1445071/150‑1 (MQ=255)
                                                                          cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCAc                                                                             <  1:1427022/150‑1 (MQ=255)
                                                                           aGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACg                                                                            <  1:3200436/150‑1 (MQ=255)
                                                                           aGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACg                                                                            <  2:737772/150‑1 (MQ=255)
                                                                               cgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                                                        >  2:3560294/1‑150 (MQ=255)
                                                                               cgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                                                        >  2:3786364/1‑150 (MQ=255)
                                                                               cgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                                                        >  2:1596389/1‑150 (MQ=255)
                                                                               cgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcga                                                                        <  1:3657812/150‑2 (MQ=255)
                                                                                gccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                                                       >  1:2996083/1‑150 (MQ=255)
                                                                                gccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                                                       >  1:4219815/1‑150 (MQ=255)
                                                                                gccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                                                       >  2:1964809/1‑150 (MQ=255)
                                                                                 ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCgg                                                                      >  2:2129659/1‑150 (MQ=255)
                                                                                     cGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAg                                                                  <  1:649649/150‑1 (MQ=255)
                                                                                     cGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAg                                                                  <  2:2150222/150‑1 (MQ=255)
                                                                                     cGGTTGTTACTTCCTGACGCTTTTCCGGGACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAg                                                                  <  1:1543284/150‑1 (MQ=255)
                                                                                       gttgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTa                                                                >  2:2850722/1‑150 (MQ=255)
                                                                                         tgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAt                                                              >  2:607075/1‑150 (MQ=255)
                                                                                          gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtg                                                             >  1:2748486/1‑150 (MQ=255)
                                                                                            tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtgtg                                                           >  2:3834117/1‑150 (MQ=255)
                                                                                             aCTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGa                                                          <  1:3477486/150‑1 (MQ=255)
                                                                                               ttCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACg                                                        >  2:1184196/1‑150 (MQ=255)
                                                                                                    gACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGAt                                                   >  1:2064504/1‑150 (MQ=255)
                                                                                                     aCGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                                                  >  1:439409/1‑150 (MQ=255)
                                                                                                      cGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCt                                                 >  2:1539094/1‑150 (MQ=255)
                                                                                                      cGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCt                                                 >  2:1539202/1‑150 (MQ=255)
                                                                                                         ttttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCa                                              >  1:4072619/1‑150 (MQ=255)
                                                                                                         ttttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCa                                              <  1:1324583/150‑1 (MQ=255)
                                                                                                         ttttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCa                                              >  1:973409/1‑150 (MQ=255)
                                                                                                             ccGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTa                                          <  1:1135574/150‑1 (MQ=255)
                                                                                                                gTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAAt                                       <  2:657608/150‑1 (MQ=255)
                                                                                                                   ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa                                    >  2:2125845/1‑150 (MQ=255)
                                                                                                                   ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGAACGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCa                                    >  1:3438208/1‑150 (MQ=255)
                                                                                                                        cagcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTa                               >  2:4258127/1‑150 (MQ=255)
                                                                                                                          gcaAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAAt                             <  2:396863/150‑1 (MQ=255)
                                                                                                                            aaaaaTGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGc                           >  2:446780/1‑150 (MQ=255)
                                                                                                                                aTGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTc                       <  2:3486913/150‑1 (MQ=255)
                                                                                                                                 tgtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTcc                      >  1:757918/1‑150 (MQ=255)
                                                                                                                                 tgtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTcc                      <  2:4056076/150‑1 (MQ=255)
                                                                                                                                  gtgGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCg                     <  2:3359314/150‑1 (MQ=255)
                                                                                                                                   tgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGc                    >  2:1912983/1‑150 (MQ=255)
                                                                                                                                   tgGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGc                    >  1:1032257/1‑150 (MQ=255)
                                                                                                                                    ggCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt                   >  2:321594/1‑150 (MQ=255)
                                                                                                                                    ggCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt                   >  1:4045302/1‑150 (MQ=255)
                                                                                                                                     gCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTc                  >  2:1851392/1‑150 (MQ=255)
                                                                                                                                      cTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTCATGCCGTCCGCTcc                 >  1:961825/1‑150 (MQ=255)
                                                                                                                                       ttCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTccc                >  1:2659238/1‑150 (MQ=255)
                                                                                                                                         cGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGc              >  2:2001427/1‑150 (MQ=255)
                                                                                                                                           tctcAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCt            <  2:1237181/150‑1 (MQ=255)
                                                                                                                                            ctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg           >  2:913750/1‑150 (MQ=255)
                                                                                                                                               aaCGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTACTGCCGTCCGCTCCCGCCTGCTc        >  2:2644157/1‑150 (MQ=255)
                                                                                                                                               aaCGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTc        >  2:2929583/1‑150 (MQ=255)
                                                                                                                                                aCGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCg       <  2:1210137/150‑1 (MQ=255)
                                                                                                                                                  ggCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGc     >  2:1801639/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAT  >  CP009273/2694694‑2694992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A