Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,694,843 G→T 100% I82I (ATC→ATA)  pdxJ ← pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,694,8430GT98.9% 595.4 / ‑1.9 183I82I (ATC→ATA) pdxJpyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (119/62);  total (120/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 183 total aligned reads displayed.

AAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTC  >  CP009273/2694698‑2694986
                                                                                                                                                 |                                                                                                                                               
aaaCCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTAt                                                                                                                                               <  1:1224384/148‑1 (MQ=255)
aaaCCTGAATCCCGGAATCTGCAAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGACTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTAt                                                                                                                                               >  2:3462776/1‑148 (MQ=255)
   ccTGAATCCCGGCATCTGCAAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGc                                                                                                                                            >  2:1422228/1‑148 (MQ=255)
    cTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGcc                                                                                                                                           <  1:27483/148‑1 (MQ=255)
      gAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAg                                                                                                                                         >  1:1498566/1‑148 (MQ=255)
      gAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCGTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAg                                                                                                                                         >  1:1498620/1‑148 (MQ=255)
       aaTCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGc                                                                                                                                        <  1:2458975/148‑1 (MQ=255)
       aaTCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGc                                                                                                                                        <  1:3490384/148‑1 (MQ=255)
       aaTCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGc                                                                                                                                        <  1:2459118/148‑1 (MQ=255)
         tCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAt                                                                                                                                      >  2:115983/1‑148 (MQ=255)
           ccGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAtct                                                                                                                                    <  1:458308/148‑1 (MQ=255)
             ggCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATctct                                                                                                                                  <  1:56560/148‑1 (MQ=255)
                 tCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCgg                                                                                                                              <  2:2301396/148‑1 (MQ=255)
                  cTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGt                                                                                                                             >  2:2307128/1‑148 (MQ=255)
                  cTGCCAGACGGTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGt                                                                                                                             >  2:2407079/1‑148 (MQ=255)
                         aCGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGcc                                                                                                                      <  1:661034/148‑1 (MQ=255)
                           gTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAt                                                                                                                    >  2:3159845/1‑148 (MQ=255)
                           gTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAt                                                                                                                    <  2:2190001/148‑1 (MQ=255)
                            tttGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAtc                                                                                                                   >  2:1571250/1‑148 (MQ=255)
                              tGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAtctc                                                                                                                 <  1:1873787/148‑1 (MQ=255)
                                     aTCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTc                                                                                                          <  1:183200/148‑1 (MQ=255)
                                         cgcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGc                                                                                                      >  2:2247927/1‑148 (MQ=255)
                                          gcATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCg                                                                                                     >  2:2168917/1‑148 (MQ=255)
                                           cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCgg                                                                                                    >  1:2964934/1‑148 (MQ=255)
                                           cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCgg                                                                                                    <  1:3257989/148‑1 (MQ=255)
                                           cATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCgg                                                                                                    >  1:457140/1‑148 (MQ=255)
                                              tttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTa                                                                                                 >  1:1049666/1‑148 (MQ=255)
                                              tttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTa                                                                                                 >  1:2320050/1‑148 (MQ=255)
                                              tttGTCACGCTGCACTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTa                                                                                                 >  1:2953428/1‑148 (MQ=255)
                                               ttGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACGTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTAt                                                                                                <  1:2255162/148‑1 (MQ=255)
                                                      gCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGt                                                                                         <  2:2378997/148‑1 (MQ=255)
                                                       cTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCg                                                                                                     <  1:1055891/135‑1 (MQ=255)
                                                       cTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTc                                                                                        <  2:207166/148‑1 (MQ=255)
                                                            cTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACg                                                                                   <  1:1006989/148‑1 (MQ=255)
                                                             tGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGc                                                                                  <  1:2170135/148‑1 (MQ=255)
                                                              gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCa                                                                                 >  1:553339/1‑148 (MQ=255)
                                                               cGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAg                                                                                >  1:974901/1‑148 (MQ=255)
                                                                  cATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGAt                                                                             <  1:1431139/148‑1 (MQ=255)
                                                                      cAGGCCGCCTTCGGTTGTTACTTCATGACGCTTTTCCGGTACCAGGCAGAAAAAATGTGGCTTCGTCTCAACGGCTATAGCCAGCATCTCTACGGACACCGCCATCACCAGATACATGCGGGTATCCAGCGTCTGACGCAGGATgagc                                                                         >  1:1918776/1‑148 (MQ=255)
                                                                       aGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCa                                                                        >  2:191106/1‑148 (MQ=255)
                                                                       aGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCa                                                                        >  2:130755/1‑148 (MQ=255)
                                                                        ggCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCAc                                                                       >  2:628263/1‑148 (MQ=255)
                                                                         gccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACg                                                                      <  2:112824/148‑1 (MQ=255)
                                                                         gccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACg                                                                      >  2:1043119/1‑148 (MQ=255)
                                                                         gccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACg                                                                      >  1:965685/1‑148 (MQ=255)
                                                                          ccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGt                                                                     <  1:1301625/148‑1 (MQ=255)
                                                                            gccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcg                                                                   <  1:3238562/148‑1 (MQ=255)
                                                                             ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                                                  >  2:1590720/1‑148 (MQ=255)
                                                                               ttCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCgg                                                                >  2:1406638/1‑148 (MQ=255)
                                                                                tCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                                               >  2:250875/1‑148 (MQ=255)
                                                                                tCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                                               >  2:314890/1‑148 (MQ=255)
                                                                                tCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGt                                                               >  1:1954332/1‑148 (MQ=255)
                                                                                 cGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTc                                                              <  2:1337228/148‑1 (MQ=255)
                                                                                  ggTTGTTACTTCCTGACGCTTTTCCGGGACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCa                                                             <  1:2504382/148‑1 (MQ=255)
                                                                                   gttgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAg                                                            >  1:2696143/1‑148 (MQ=255)
                                                                                      gttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTaa                                                         <  1:592796/148‑1 (MQ=255)
                                                                                       ttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAt                                                        >  1:119667/1‑148 (MQ=255)
                                                                                        tACTTCCTGACGCTTTTCCGGGACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtg                                                       <  1:713577/148‑1 (MQ=255)
                                                                                           ttCCTGACTCTTTTCCGGTACCATGCAGAAAAAATGTCGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCTGTCACCGCCATCTCCAGATTCTTGCGTGTATCCAGCGTCTGACGAAGGATGCGCAAGTCGCTGTCAGTAATGTGa                                                    <  2:1818016/148‑1 (MQ=255)
                                                                                           ttCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGa                                                    >  1:1495891/1‑148 (MQ=255)
                                                                                                  cGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGAt                                             <  2:408209/148‑1 (MQ=255)
                                                                                                  cGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGAt                                             <  1:2171304/148‑1 (MQ=255)
                                                                                                      tttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTc                                         >  1:1518338/1‑148 (MQ=255)
                                                                                                      tttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTc                                         >  1:1520345/1‑148 (MQ=255)
                                                                                                         ccGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACg                                      >  1:1390538/1‑148 (MQ=255)
                                                                                                            gTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTaa                                   >  1:3139803/1‑148 (MQ=255)
                                                                                                              aCCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCAc                                       <  2:953679/142‑1 (MQ=255)
                                                                                                              aCCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCAc                                       >  1:953679/1‑142 (MQ=255)
                                                                                                               ccAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATg                                <  1:1422228/148‑1 (MQ=255)
                                                                                                                cAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGc                               >  2:1648339/1‑148 (MQ=255)
                                                                                                                cAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGc                               >  2:2974097/1‑148 (MQ=255)
                                                                                                                      gcaAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTa                         <  2:764959/148‑1 (MQ=255)
                                                                                                                       caAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTaa                        <  1:2245713/148‑1 (MQ=255)
                                                                                                                       caAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTaa                        >  1:3004196/1‑148 (MQ=255)
                                                                                                                        aaaaaTGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAAt                       >  2:1768206/1‑148 (MQ=255)
                                                                                                                         aaaaTGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATg                      >  2:1745328/1‑148 (MQ=255)
                                                                                                                          aaaTGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGc                     >  1:3427633/1‑148 (MQ=255)
                                                                                                                          aaaTGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGc                     >  1:1265978/1‑148 (MQ=255)
                                                                                                                             tgtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGt                  >  2:629722/1‑148 (MQ=255)
                                                                                                                              gtgGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTc                 >  1:2193573/1‑148 (MQ=255)
                                                                                                                                ggCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCg               >  2:1323538/1‑148 (MQ=255)
                                                                                                                                  cTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt             >  1:2036506/1‑148 (MQ=255)
                                                                                                                                  cTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt             >  1:1707/1‑148 (MQ=255)
                                                                                                                                  cTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt             >  1:263505/1‑148 (MQ=255)
                                                                                                                                  cTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCt             >  2:2860901/1‑148 (MQ=255)
                                                                                                                                    tCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTcc           >  2:137551/1‑148 (MQ=255)
                                                                                                                                       tctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTACTGCCGTCCGCTCCCGc        >  2:2734020/1‑148 (MQ=255)
                                                                                                                                       tctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGc        >  1:3200504/1‑148 (MQ=255)
                                                                                                                                       tctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGc        >  2:2733271/1‑148 (MQ=255)
                                                                                                                                       tctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGc        >  2:580814/1‑148 (MQ=255)
                                                                                                                                       tctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAAAGCCGTCCGCTCCCGc        >  2:580864/1‑148 (MQ=255)
                                                                                                                                        ctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGcc       >  2:2970248/1‑148 (MQ=255)
                                                                                                                                        ctcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGcc       <  2:553339/148‑1 (MQ=255)
                                                                                                                                         tcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCt      >  2:3352316/1‑148 (MQ=255)
                                                                                                                                         tcAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCt      <  1:1839432/148‑1 (MQ=255)
                                                                                                                                          cAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTg     >  2:3046048/1‑148 (MQ=255)
                                                                                                                                            aCGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCt   >  1:292932/1‑148 (MQ=255)
                                                                                                                                            aCGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCt   >  1:292652/1‑148 (MQ=255)
                                                                                                                                             cGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTc  >  1:1131828/1‑148 (MQ=255)
                                                                                                                                             cGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTc  >  2:101453/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTC  >  CP009273/2694698‑2694986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAT  >  CP009273/2694694‑2694992
                                                                                                                                                     |                                                                                                                                                     
AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCT                                                                                                                                                       >  SRR19973065.82417/1‑150 (MQ=60)
AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCT                                                                                                                                                       >  SRR19973065.82397/1‑150 (MQ=60)
AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCT                                                                                                                                                       >  SRR19973065.3047763/1‑150 (MQ=60)
AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCT                                                                                                                                                       >  SRR19973065.1283819/1‑150 (MQ=60)
 GAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTA                                                                                                                                                      <  SRR19973065.3121781/150‑1 (MQ=60)
 GAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTA                                                                                                                                                      <  SRR19973065.1740359/150‑1 (MQ=60)
 GAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTA                                                                                                                                                      <  SRR19973065.3377213/150‑1 (MQ=60)
 GAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTA                                                                                                                                                      <  SRR19973065.1367948/150‑1 (MQ=60)
 GAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTA                                                                                                                                                      <  SRR19973065.691426/150‑1 (MQ=60)
   GAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATC                                                                                                                                                    <  SRR19973065.651713/150‑1 (MQ=60)
    AAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCG                                                                                                                                                   <  SRR19973065.1232367/150‑1 (MQ=60)
    AAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCG                                                                                                                                                   <  SRR19973065.1963774/150‑1 (MQ=60)
        CTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAG                                                                                                                                               <  SRR19973065.27843/150‑1 (MQ=60)
        CTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAG                                                                                                                                               >  SRR19973065.1508261/1‑150 (MQ=60)
        CTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAG                                                                                                                                               >  SRR19973065.1508222/1‑150 (MQ=60)
        CTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGGACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAG                                                                                                                                               <  SRR19973065.26827/150‑1 (MQ=60)
        CTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCGTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAG                                                                                                                                               >  SRR19973065.1508277/1‑150 (MQ=60)
         TGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGC                                                                                                                                              >  SRR19973065.2179669/1‑150 (MQ=60)
           AATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAT                                                                                                                                            <  SRR19973065.2474472/150‑1 (MQ=60)
           AATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAT                                                                                                                                            >  SRR19973065.1334166/1‑150 (MQ=60)
           AATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAT                                                                                                                                            <  SRR19973065.2474616/150‑1 (MQ=60)
           AATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAT                                                                                                                                            <  SRR19973065.3511608/150‑1 (MQ=60)
           AATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCAT                                                                                                                                            <  SRR19973065.2474260/150‑1 (MQ=60)
               CCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCT                                                                                                                                        <  SRR19973065.461468/150‑1 (MQ=60)
               CCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCT                                                                                                                                        <  SRR19973065.461469/150‑1 (MQ=60)
                 GGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTC                                                                                                                                      <  SRR19973065.57165/150‑1 (MQ=60)
                          CAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGC                                                                                                                             >  SRR19973065.1834733/1‑150 (MQ=60)
                             ACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCAT                                                                                                                          <  SRR19973065.665410/150‑1 (MQ=60)
                              CGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATC                                                                                                                         <  SRR19973065.681082/150‑1 (MQ=60)
                                  TGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCA                                                                                                                     <  SRR19973065.1886124/150‑1 (MQ=60)
                                        CATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCA                                                                                                               >  SRR19973065.3443361/1‑150 (MQ=60)
                                         ATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCAT                                                                                                              <  SRR19973065.2510707/150‑1 (MQ=60)
                                         ATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCAT                                                                                                              <  SRR19973065.184647/150‑1 (MQ=60)
                                             CGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGG                                                                                                          >  SRR19973065.460298/1‑150 (MQ=60)
                                             CGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGG                                                                                                          >  SRR19973065.2983054/1‑150 (MQ=60)
                                              tcttccgatctCGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGG                                                                                                         <  SRR19973065.1062895/139‑1 (MQ=60)
                                               CATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGT                                                                                                        <  SRR19973065.3277711/150‑1 (MQ=60)
                                                ATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTA                                                                                                       >  SRR19973065.2971431/1‑150 (MQ=60)
                                                ATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTA                                                                                                       >  SRR19973065.2334590/1‑150 (MQ=60)
                                                ATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTA                                                                                                       >  SRR19973065.1056617/1‑150 (MQ=60)
                                                ATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTA                                                                                                       >  SRR19973065.1056728/1‑150 (MQ=60)
                                                ATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTA                                                                                                       >  SRR19973065.1207723/1‑150 (MQ=60)
                                                ATTTTGTCACGCTGCACTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTA                                                                                                       >  SRR19973065.2971437/1‑150 (MQ=60)
                                                   TTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACGTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCC                                                                                                    <  SRR19973065.366746/150‑1 (MQ=60)
                                                   TTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACGTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCC                                                                                                    <  SRR19973065.2269444/150‑1 (MQ=60)
                                                    TGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCA                                                                                                   <  SRR19973065.1522384/150‑1 (MQ=60)
                                                               CCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGC                                                                                        >  SRR19973065.2261796/1‑150 (MQ=60)
                                                                CTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCA                                                                                       <  SRR19973065.1013673/150‑1 (MQ=60)
                                                                CTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCA                                                                                       >  SRR19973065.556885/1‑150 (MQ=60)
                                                                 TGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAG                                                                                      >  SRR19973065.981376/1‑150 (MQ=60)
                                                                 TGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAG                                                                                      >  SRR19973065.981362/1‑150 (MQ=60)
                                                                 TGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAG                                                                                      <  SRR19973065.2184067/150‑1 (MQ=60)
                                                                    GACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGAT                                                                                   <  SRR19973065.584044/150‑1 (MQ=60)
                                                                      CATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGC                                                                                 <  SRR19973065.1440445/150‑1 (MQ=60)
                                                                       ATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCCTCTCCAGATTCAAGCGGGTATCCAGCGTCTGACGCAGGATGCG                                                                                >  SRR19973065.3113654/1‑150 (MQ=60)
                                                                        TCCAGGCCGCCTTCGGTTGTTACTTCATGACGCTTTTCCGGTACCAGGCAGAAAAAATGTGGCTTCGTCTCAACGGCTATAGCCAGCATCTCTACGGACACCGCCATCACCAGATACATGCGGGTATCCAGCGTCTGACGCAGGATGAGC                                                                               >  SRR19973065.1931361/1‑150 (MQ=60)
                                                                         CCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCA                                                                              >  SRR19973065.2250174/1‑150 (MQ=60)
                                                                           AGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACG                                                                            >  SRR19973065.972093/1‑150 (MQ=60)
                                                                           AGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACG                                                                            >  SRR19973065.972047/1‑150 (MQ=60)
                                                                              CCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCG                                                                         <  SRR19973065.1310199/150‑1 (MQ=60)
                                                                              CCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTATCAACGGCTATCGCCGGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCG                                                                         <  SRR19973065.3274165/150‑1 (MQ=60)
                                                                                GCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCCACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCG                                                                       >  SRR19973065.2982028/1‑150 (MQ=60)
                                                                                GCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCG                                                                       <  SRR19973065.3258065/150‑1 (MQ=60)
                                                                                  CTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGT                                                                     >  SRR19973065.1967103/1‑150 (MQ=60)
                                                                                     CGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAG                                                                  >  SRR19973065.2712890/1‑150 (MQ=60)
                                                                                      GGTTGTTACTTCCTGACGCTTTTCCGGGACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGT                                                                 <  SRR19973065.2520174/150‑1 (MQ=60)
                                                                                      GGTTGTTACTTCCTGACGCTTTTCCGGGACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGT                                                                 <  SRR19973065.2520123/150‑1 (MQ=60)
                                                                                         TGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAT                                                              >  SRR19973065.120721/1‑150 (MQ=60)
                                                                                         TGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAT                                                              >  SRR19973065.1906917/1‑150 (MQ=60)
                                                                                          GTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATG                                                             <  SRR19973065.596459/150‑1 (MQ=60)
                                                                                          GTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATG                                                             <  SRR19973065.658317/150‑1 (MQ=60)
                                                                                            TACTTCCTGACGCTTTTCCGGGACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTG                                                           <  SRR19973065.718352/150‑1 (MQ=60)
                                                                                             ACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGA                                                          >  SRR19973065.1505511/1‑150 (MQ=60)
                                                                                                      CGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCT                                                 <  SRR19973065.2185238/150‑1 (MQ=60)
                                                                                                        CTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTC                                               >  SRR19973065.1528305/1‑150 (MQ=60)
                                                                                                        CTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTC                                               >  SRR19973065.1528269/1‑150 (MQ=60)
                                                                                                        CTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTC                                               >  SRR19973065.1530305/1‑150 (MQ=60)
                                                                                                          TTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCAC                                             <  SRR19973065.1463757/150‑1 (MQ=60)
                                                                                                           TTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACG                                            >  SRR19973065.1399670/1‑150 (MQ=60)
                                                                                                              CGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAA                                         >  SRR19973065.3158891/1‑150 (MQ=60)
                                                                                                              CGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAA                                         >  SRR19973065.3158989/1‑150 (MQ=60)
                                                                                                                GTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAGAT                                       >  SRR19973065.959983/1‑150 (MQ=60)
                                                                                                                   CCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCA                                    <  SRR19973065.2797043/150‑1 (MQ=60)
                                                                                                                   CCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCA                                    <  SRR19973065.1431503/150‑1 (MQ=60)
                                                                                                                   CCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCA                                    >  SRR19973065.1418066/1‑150 (MQ=60)
                                                                                                                     AGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACG                                  <  SRR19973065.1641821/150‑1 (MQ=60)
                                                                                                                     AGGCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACG                                  >  SRR19973065.2730384/1‑150 (MQ=60)
                                                                                                                       GCAGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGT                                <  SRR19973065.2290097/150‑1 (MQ=60)
                                                                                                                         AGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAA                              >  SRR19973065.1285188/1‑150 (MQ=60)
                                                                                                                         AGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAA                              >  SRR19973065.3022603/1‑150 (MQ=60)
                                                                                                                         AGCAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAA                              >  SRR19973065.3021737/1‑150 (MQ=60)
                                                                                                                           CAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTACTG                            >  SRR19973065.2021173/1‑150 (MQ=60)
                                                                                                                           CAAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATG                            <  SRR19973065.2259968/150‑1 (MQ=60)
                                                                                                                            AAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGC                           >  SRR19973065.3448567/1‑150 (MQ=60)
                                                                                                                            AAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGC                           >  SRR19973065.3448551/1‑150 (MQ=60)
                                                                                                                            AAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGC                           <  SRR19973065.117004/150‑1 (MQ=60)
                                                                                                                            AAAAATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGC                           >  SRR19973065.1274321/1‑150 (MQ=60)
                                                                                                                                ATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGCTCTTCACGTAAATGCACGGTAATGCCGTC                       >  SRR19973065.2207124/1‑150 (MQ=60)
                                                                                                                                ATGTGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTC                       >  SRR19973065.2207632/1‑150 (MQ=60)
                                                                                                                                   TGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGC                    >  SRR19973065.1433206/1‑150 (MQ=60)
                                                                                                                                   TGGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGC                    >  SRR19973065.565540/1‑150 (MQ=60)
                                                                                                                                    GGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCT                   >  SRR19973065.2049557/1‑150 (MQ=60)
                                                                                                                                    GGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCT                   >  SRR19973065.265312/1‑150 (MQ=60)
                                                                                                                                    GGCTTCGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCT                   >  SRR19973065.1726/1‑150 (MQ=60)
                                                                                                                                         CGTCTCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGC              >  SRR19973065.3219777/1‑150 (MQ=60)
                                                                                                                                             TCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGC          <  SRR19973065.1851592/150‑1 (MQ=60)
                                                                                                                                             TCAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGC          <  SRR19973065.1851562/150‑1 (MQ=60)
                                                                                                                                              CAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCT         >  SRR19973065.294826/1‑150 (MQ=60)
                                                                                                                                              CAACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCT         >  SRR19973065.294546/1‑150 (MQ=60)
                                                                                                                                               AACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTC        >  SRR19973065.1139175/1‑150 (MQ=60)
                                                                                                                                               AACGGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTC        >  SRR19973065.1139171/1‑150 (MQ=60)
                                                                                                                                                  GGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGC     >  SRR19973065.3367879/1‑150 (MQ=60)
                                                                                                                                                  GGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGC     >  SRR19973065.3367854/1‑150 (MQ=60)
                                                                                                                                                  GGCTATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGC     >  SRR19973065.75652/1‑150 (MQ=60)
                                                                                                                                                     TATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAT  >  SRR19973065.2366306/1‑150 (MQ=60)
                                                                                                                                                     TATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAT  >  SRR19973065.2366321/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGAGAAACCTGAATCCCGGCATCTGCCAGACGTTTGCAGGCATCGCGCATTTTGTCACGCTGCCCTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAT  >  CP009273/2694694‑2694992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑