Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 415,049 G→T M1M (ATG→ATT) † brnQ → branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273415,0490GT99.5% 568.8 / NA 181M1M (ATG→ATT) †brnQbranched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (79/101);  total (79/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

ACGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAT  >  CP009273/414902‑415195
                                                                                                                                                   |                                                                                                                                                  
 cgcgGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTAcc                                                                                                                                                 <  2:1498036/150‑1 (MQ=255)
    ggTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCAt                                                                                                                                              >  2:808103/1‑150 (MQ=255)
      tAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCa                                                                                                                                            <  2:3523050/150‑1 (MQ=255)
        aaTCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAAt                                                                                                                                          <  2:3429807/150‑1 (MQ=255)
         aTCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAAtt                                                                                                                                         >  1:3272365/1‑150 (MQ=255)
            gAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAg                                                                                                                                      <  2:657187/150‑1 (MQ=255)
            gAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAg                                                                                                                                      >  1:2164944/1‑150 (MQ=255)
             aaaaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGa                                                                                                                                     <  2:856462/150‑1 (MQ=255)
               aaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATc                                                                                                                                   <  2:9619/150‑1 (MQ=255)
               aaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATc                                                                                                                                   >  1:3404983/1‑150 (MQ=255)
               aaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATc                                                                                                                                   <  2:1249348/150‑1 (MQ=255)
               aaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATc                                                                                                                                   <  2:1571508/150‑1 (MQ=255)
               aaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATc                                                                                                                                   <  2:718544/150‑1 (MQ=255)
                 aCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATcgc                                                                                                                                 <  2:894506/150‑1 (MQ=255)
                 aCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATcgc                                                                                                                                 <  1:334720/150‑1 (MQ=255)
                   tATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATcgcgc                                                                                                                               >  1:3200494/1‑150 (MQ=255)
                    aTTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATcgcgcg                                                                                                                              >  1:203095/1‑150 (MQ=255)
                        ttcGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGatat                                                                                                                          <  1:2773034/150‑1 (MQ=255)
                            ccgcGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATatcatc                                                                                                                      <  1:2964832/150‑1 (MQ=255)
                              gcgcCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGc                                                                                                                    >  2:2050890/1‑150 (MQ=255)
                               cgcCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGct                                                                                                                   <  1:557117/150‑1 (MQ=255)
                               cgcCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGct                                                                                                                   >  1:3460580/1‑150 (MQ=255)
                                gcatGGTTGGGAGTATTTCCCGATAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGctc                                                                                                                  <  2:2290641/147‑1 (MQ=255)
                                gcCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGctc                                                                                                                  >  2:128543/1‑150 (MQ=255)
                                   tGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTgg                                                                                                               >  2:1167859/1‑150 (MQ=255)
                                      ttGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCt                                                                                                            <  2:839334/150‑1 (MQ=255)
                                       tGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCtt                                                                                                           >  2:3337793/1‑150 (MQ=255)
                                          gAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTAt                                                                                                        <  1:1965854/150‑1 (MQ=255)
                                          gAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTAt                                                                                                        <  1:1878851/150‑1 (MQ=255)
                                           aGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATg                                                                                                       <  2:3272365/150‑1 (MQ=255)
                                           aGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATg                                                                                                       <  1:942332/150‑1 (MQ=255)
                                             tATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGAc                                                                                                     >  2:3413488/1‑150 (MQ=255)
                                               tttCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACAt                                                                                                   >  1:2200649/1‑150 (MQ=255)
                                                   ccGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGc                                                                                               <  1:3711615/150‑1 (MQ=255)
                                                    cGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCg                                                                                              >  2:100425/1‑150 (MQ=255)
                                                      cTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCgtt                                                                                            <  1:1521067/150‑1 (MQ=255)
                                                        aaaaTTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCgttgt                                                                                          >  2:2779534/1‑150 (MQ=255)
                                                          aaTTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtc                                                                                        <  2:1465783/150‑1 (MQ=255)
                                                          aaTTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtc                                                                                        <  2:1252577/150‑1 (MQ=255)
                                                          aaTTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtc                                                                                        <  2:1873101/150‑1 (MQ=255)
                                                              gTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtcgtcg                                                                                    <  1:748938/150‑1 (MQ=255)
                                                              gTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtcgtcg                                                                                    <  2:1430705/150‑1 (MQ=255)
                                                               tttAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCgg                                                                                   >  1:2837105/1‑150 (MQ=255)
                                                                ttAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGgc                                                                                  <  1:416450/150‑1 (MQ=255)
                                                                    atatACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAg                                                                              >  2:2832641/1‑150 (MQ=255)
                                                                    atatACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAg                                                                              >  2:467246/1‑150 (MQ=255)
                                                                        aCCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTaa                                                                          >  1:2381764/1‑150 (MQ=255)
                                                                          cGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACa                                                                        <  2:2725494/150‑1 (MQ=255)
                                                                            cTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACatt                                                                      <  2:3317756/150‑1 (MQ=255)
                                                                            cTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACatt                                                                      <  1:3273088/150‑1 (MQ=255)
                                                                            cTGTATCATCCCCAGGGATTGACACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACatt                                                                      >  1:1512273/1‑150 (MQ=255)
                                                                              gTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACattat                                                                    >  2:3728593/1‑150 (MQ=255)
                                                                               tATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACattatt                                                                   <  1:1195606/150‑1 (MQ=255)
                                                                               tATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACattatt                                                                   <  2:801792/150‑1 (MQ=255)
                                                                                     ccccAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCt                                                             <  1:3377832/150‑1 (MQ=255)
                                                                                     ccccAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGAGATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCt                                                             <  2:2003764/150‑1 (MQ=255)
                                                                                      cccAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTc                                                            >  1:2677739/1‑150 (MQ=255)
                                                                                       ccAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTcc                                                           <  2:2198936/150‑1 (MQ=255)
                                                                                        cAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCa                                                          >  2:2194237/1‑150 (MQ=255)
                                                                                           ggATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCACTg                                                       <  2:2411206/150‑1 (MQ=255)
                                                                                           ggATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATg                                                       <  2:1750455/150‑1 (MQ=255)
                                                                                            gATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATgg                                                      >  1:318251/1‑150 (MQ=255)
                                                                                             aTTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGt                                                     <  2:3146710/150‑1 (MQ=255)
                                                                                             aTTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGt                                                     <  2:213424/150‑1 (MQ=255)
                                                                                              ttGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTc                                                    <  1:772910/150‑1 (MQ=255)
                                                                                                 gCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGc                                                 >  2:1771601/1‑150 (MQ=255)
                                                                                                  cacaAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCt                                                <  2:2959624/150‑1 (MQ=255)
                                                                                                     aaaaaTTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAc                                             <  1:3615977/150‑1 (MQ=255)
                                                                                                       aaaTTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAcag                                           <  2:3373469/150‑1 (MQ=255)
                                                                                                       aaaTTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAcag                                           <  2:3200720/150‑1 (MQ=255)
                                                                                                       aaaTTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAcag                                           <  2:3200291/150‑1 (MQ=255)
                                                                                                           ttAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAcaggcag                                       >  1:2815831/1‑150 (MQ=255)
                                                                                                             aaCGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACaggcaggc                                     <  2:2111438/150‑1 (MQ=255)
                                                                                                             aaCGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACaggcaggc                                     <  2:663058/150‑1 (MQ=255)
                                                                                                             aaCGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACaggcaggc                                     <  2:1153758/150‑1 (MQ=255)
                                                                                                               cGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGa                                   <  1:3202782/150‑1 (MQ=255)
                                                                                                               cGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGa                                   >  2:1234310/1‑150 (MQ=255)
                                                                                                                  tACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAaca                                >  1:1328208/1‑150 (MQ=255)
                                                                                                                   acaacaCCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAacac                               <  2:3241150/150‑1 (MQ=255)
                                                                                                                   acaacaCCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAacac                               <  2:3404983/150‑1 (MQ=255)
                                                                                                                   acaacaCCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAacac                               >  2:2626115/1‑150 (MQ=255)
                                                                                                                   acaacaCCACATCCACAGGCAGTATGATGTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAacac                               >  2:2626285/1‑150 (MQ=255)
                                                                                                                    caacaCCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACg                              >  1:1635048/1‑150 (MQ=255)
                                                                                                                     aacaCCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGt                             >  1:2337968/1‑150 (MQ=255)
                                                                                                                       caccacATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCt                           <  1:100425/150‑1 (MQ=255)
                                                                                                                               ccACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCg                   <  2:2976977/150‑1 (MQ=255)
                                                                                                                                  taGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCa                >  1:1826012/2‑150 (MQ=255)
                                                                                                                                    ggCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCAtt              >  2:2968238/1‑150 (MQ=255)
                                                                                                                                    ggCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCAtt              <  1:128543/150‑1 (MQ=255)
                                                                                                                                     gCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTc             <  1:3384578/150‑1 (MQ=255)
                                                                                                                                      cAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCg            >  2:185451/1‑150 (MQ=255)
                                                                                                                                       aGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCgg           >  1:3789892/1‑150 (MQ=255)
                                                                                                                                       aGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCgg           >  2:2330840/1‑150 (MQ=255)
                                                                                                                                       aGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTATATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCgg           >  2:3668924/1‑150 (MQ=255)
                                                                                                                                           tGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTc       >  1:3406963/1‑150 (MQ=255)
                                                                                                                                           tGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTc       <  1:952262/150‑1 (MQ=255)
                                                                                                                                             aTTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCAGTCGGCTTCCt     >  1:2988667/1‑150 (MQ=255)
                                                                                                                                                tATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAt  <  1:1459545/150‑1 (MQ=255)
                                                                                                                                                tATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAt  >  1:529047/1‑150 (MQ=255)
                                                                                                                                                tATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAt  <  1:2373848/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAT  >  CP009273/414902‑415195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTA  >  CP009273/414900‑415197
                                                                                                                                                     |                                                                                                                                                    
TAACGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATT                                                                                                                                                      >  SRR19973061.3684777/1‑150 (MQ=60)
          AATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAAT                                                                                                                                            >  SRR19973061.3389010/1‑150 (MQ=60)
           ATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATT                                                                                                                                           <  SRR19973061.1251899/150‑1 (MQ=60)
           ATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATT                                                                                                                                           >  SRR19973061.3279832/1‑150 (MQ=60)
           ATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATT                                                                                                                                           >  SRR19973061.3280795/1‑150 (MQ=60)
              GAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAG                                                                                                                                        >  SRR19973061.2170381/1‑150 (MQ=60)
                 AAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATC                                                                                                                                     >  SRR19973061.3413729/1‑150 (MQ=60)
                   ACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGC                                                                                                                                   <  SRR19973061.335599/150‑1 (MQ=60)
                    CTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCG                                                                                                                                  <  SRR19973061.3407117/150‑1 (MQ=60)
                     TATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGC                                                                                                                                 >  SRR19973061.3208944/1‑150 (MQ=60)
                     TATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGC                                                                                                                                 >  SRR19973061.3208513/1‑150 (MQ=60)
                     TATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGC                                                                                                                                 >  SRR19973061.3208716/1‑150 (MQ=60)
                      ATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCG                                                                                                                                >  SRR19973061.203700/1‑150 (MQ=60)
                        TCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGAT                                                                                                                              <  SRR19973061.3320007/150‑1 (MQ=60)
                          TTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGTTTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATAT                                                                                                                            <  SRR19973061.2801160/150‑1 (MQ=60)
                          TTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATAT                                                                                                                            <  SRR19973061.2780171/150‑1 (MQ=60)
                           TCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATC                                                                                                                           >  SRR19973061.2198853/1‑150 (MQ=60)
                              CCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATC                                                                                                                        <  SRR19973061.2972482/150‑1 (MQ=60)
                              CCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATC                                                                                                                        <  SRR19973061.1342390/150‑1 (MQ=60)
                               CGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCG                                                                                                                       <  SRR19973061.277083/150‑1 (MQ=60)
                                 CGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCT                                                                                                                     >  SRR19973061.25853/1‑150 (MQ=60)
                                 CGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCT                                                                                                                     <  SRR19973061.557368/150‑1 (MQ=60)
                                 CGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCT                                                                                                                     <  SRR19973061.558584/150‑1 (MQ=60)
                                 CGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCT                                                                                                                     >  SRR19973061.3469452/1‑150 (MQ=60)
                                         TGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTT                                                                                                             >  SRR19973061.2335991/1‑150 (MQ=60)
                                         TGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTT                                                                                                             >  SRR19973061.664764/1‑150 (MQ=60)
                                            GAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTAT                                                                                                          <  SRR19973061.1970770/150‑1 (MQ=60)
                                            GAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTAT                                                                                                          <  SRR19973061.1883530/150‑1 (MQ=60)
                                            GAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTAT                                                                                                          <  SRR19973061.1883523/150‑1 (MQ=60)
                                             AGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATG                                                                                                         <  SRR19973061.944723/150‑1 (MQ=60)
                                                 TTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACAT                                                                                                     >  SRR19973061.2206178/1‑150 (MQ=60)
                                                   TCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTT                                                                                                   <  SRR19973061.1814647/150‑1 (MQ=60)
                                                     CCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGC                                                                                                 <  SRR19973061.3721105/150‑1 (MQ=60)
                                                       GCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGT                                                                                               >  SRR19973061.1480002/1‑150 (MQ=60)
                                                        CTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTT                                                                                              <  SRR19973061.1524870/150‑1 (MQ=60)
                                                        CTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTT                                                                                              <  SRR19973061.1524865/150‑1 (MQ=60)
                                                                GTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCG                                                                                      <  SRR19973061.750906/150‑1 (MQ=60)
                                                                 TTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGG                                                                                     >  SRR19973061.934420/1‑150 (MQ=60)
                                                                 TTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGG                                                                                     >  SRR19973061.2844371/1‑150 (MQ=60)
                                                                  TTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGC                                                                                    <  SRR19973061.417583/150‑1 (MQ=60)
                                                                          ACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAA                                                                            >  SRR19973061.2387834/1‑150 (MQ=60)
                                                                             GCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACAT                                                                         >  SRR19973061.3429842/1‑150 (MQ=60)
                                                                              CTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATT                                                                        <  SRR19973061.2881596/150‑1 (MQ=60)
                                                                              CTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATT                                                                        <  SRR19973061.3281518/150‑1 (MQ=60)
                                                                              CTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATT                                                                        <  SRR19973061.1327200/150‑1 (MQ=60)
                                                                              CTGTATCATCCCCAGGGATTGACACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATT                                                                        >  SRR19973061.1516059/1‑150 (MQ=60)
                                                                                 TATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATT                                                                     <  SRR19973061.1198617/150‑1 (MQ=60)
                                                                                  ATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTT                                                                    <  SRR19973061.3167177/150‑1 (MQ=60)
                                                                                       CCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCT                                                               <  SRR19973061.3386514/150‑1 (MQ=60)
                                                                                        CCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTC                                                              >  SRR19973061.3797566/1‑150 (MQ=60)
                                                                                        CCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTC                                                              >  SRR19973061.2684631/1‑150 (MQ=60)
                                                                                              GATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGG                                                        >  SRR19973061.319080/1‑150 (MQ=60)
                                                                                                TTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTC                                                      <  SRR19973061.774854/150‑1 (MQ=60)
                                                                                                TTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTC                                                      <  SRR19973061.774947/150‑1 (MQ=60)
                                                                                                  GGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGG                                                    >  SRR19973061.1872836/1‑150 (MQ=60)
                                                                                                      CAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTTTTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTA                                                <  SRR19973061.2365861/150‑1 (MQ=60)
                                                                                                       AAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAC                                               <  SRR19973061.3625226/150‑1 (MQ=60)
                                                                                                          AATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGG                                            >  SRR19973061.3534079/1‑150 (MQ=60)
                                                                                                           ATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGC                                           >  SRR19973061.335712/1‑150 (MQ=60)
                                                                                                             TTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAG                                         >  SRR19973061.2823058/1‑150 (MQ=60)
                                                                                                             TTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATAACAACGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCTGCGCAGGTAACATTAATTTCCCTCCAATGGTCGGCTTACAGGCAG                                         >  SRR19973061.2823103/1‑150 (MQ=60)
                                                                                                              TAACGTTACAACACCACATCCACAGGCAGAATGATTTATTACCCATCAATTAAGATCGCGCGATAACATCGCTCTGGGCTTTATGACAATTGCGATGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGTCAGG                                        >  SRR19973061.371741/1‑150 (MQ=60)
                                                                                                                 CGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGA                                     <  SRR19973061.3211013/150‑1 (MQ=60)
                                                                                                                    TACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACA                                  >  SRR19973061.1331546/1‑150 (MQ=60)
                                                                                                                      CAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACG                                >  SRR19973061.1639142/1‑150 (MQ=60)
                                                                                                                       AACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGT                               >  SRR19973061.2343904/1‑150 (MQ=60)
                                                                                                                         CACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCT                             <  SRR19973061.100755/150‑1 (MQ=60)
                                                                                                                                    TAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCA                  >  SRR19973061.1830587/1‑150 (MQ=60)
                                                                                                                                     AGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCAT                 <  SRR19973061.2056051/150‑1 (MQ=60)
                                                                                                                                      GGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATT                <  SRR19973061.128959/150‑1 (MQ=60)
                                                                                                                                       GCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTC               <  SRR19973061.3393272/150‑1 (MQ=60)
                                                                                                                                         AGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGG             >  SRR19973061.3799549/1‑150 (MQ=60)
                                                                                                                                           TATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCT           <  SRR19973061.97171/150‑1 (MQ=60)
                                                                                                                                             TGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTC         >  SRR19973061.3415713/1‑150 (MQ=60)
                                                                                                                                             TGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTC         <  SRR19973061.954666/150‑1 (MQ=60)
                                                                                                                                               ATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCAGTCGGCTTCCT       >  SRR19973061.2996381/1‑150 (MQ=60)
                                                                                                                                                  TATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAT    >  SRR19973061.530446/1‑150 (MQ=60)
                                                                                                                                                  TATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAT    >  SRR19973061.530440/1‑150 (MQ=60)
                                                                                                                                                  TATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAT    <  SRR19973061.2379896/150‑1 (MQ=60)
                                                                                                                                                  TATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAT    <  SRR19973061.1463223/150‑1 (MQ=60)
                                                                                                                                                   ATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATT   >  SRR19973061.2560359/1‑150 (MQ=60)
                                                                                                                                                    TTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTA  >  SRR19973061.346486/1‑150 (MQ=60)
                                                                                                                                                    TTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTA  <  SRR19973061.2655291/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TAACGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTA  >  CP009273/414900‑415197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑