Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 415,049 G→T 24.4% M1M (ATG→ATT) † brnQ → branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273415,0490GT24.4% 613.8 / 140.6 269M1M (ATG→ATT) †brnQbranched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
Reads supporting (aligned to +/- strand):  ref base G (97/105);  new base T (28/39);  total (125/144)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

TAACGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTA  >  CP009273/414900‑415197
                                                                                                                                                     |                                                                                                                                                    
tAACGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATg                                                                                                                                                      <  2:4689762/150‑1 (MQ=255)
 aaCGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGa                                                                                                                                                     <  1:2991542/150‑1 (MQ=255)
      ggTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCAt                                                                                                                                                >  1:3916580/1‑150 (MQ=255)
       gTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATc                                                                                                                                               >  2:3087410/1‑150 (MQ=255)
        tataTCGAAAAACTTTTCGTCGCCGCGCCTGGTTGGGTGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGTTTGGCACAAAAATTTAACGTTACATCACCACATACACAGGCAGTATGTTTTATGACCCATCa                                                                                                                                              <  1:864696/147‑1 (MQ=255)
          aaTCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAAt                                                                                                                                            >  2:1707358/1‑150 (MQ=255)
          aaTCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAAt                                                                                                                                            >  2:2185347/1‑150 (MQ=255)
           aTCGAAAAACTATTCTTCGCCGCGCCTGGATGGGAGTATATCCCGCGAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACACAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAAtt                                                                                                                                           >  2:4943960/1‑150 (MQ=255)
               aaaaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTCTGATTTATTACCCATCAATTAAGa                                                                                                                                       >  2:3215819/1‑150 (MQ=255)
                 aaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATc                                                                                                                                     <  2:1423490/150‑1 (MQ=255)
                 aaaCTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATGTATGACCCATCAATTAAGATc                                                                                                                                     >  2:2824642/1‑150 (MQ=255)
                     tATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATcgcgc                                                                                                                                 >  1:4119657/1‑150 (MQ=255)
                     tATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATcgcgc                                                                                                                                 >  1:1456952/1‑150 (MQ=255)
                      aTTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATcgcgcg                                                                                                                                <  2:229074/150‑1 (MQ=255)
                            cgccgcGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATatca                                                                                                                          >  1:3716521/1‑150 (MQ=255)
                              ccgcGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATatcatc                                                                                                                        >  2:4557724/1‑150 (MQ=255)
                               cgcgCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCg                                                                                                                       >  2:2652271/1‑150 (MQ=255)
                                gcgcCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGc                                                                                                                      >  1:721604/1‑150 (MQ=255)
                                 cgcCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGct                                                                                                                     <  2:3483967/150‑1 (MQ=255)
                                 cgcCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCTTCAATTAAGATCGCGCGATATCATCGct                                                                                                                     >  2:3558860/1‑150 (MQ=255)
                                  gcCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCAACCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGAATGATATATGACCCATCAATTAAGAACGCGCGATATCATCGctc                                                                                                                    >  2:2146355/1‑150 (MQ=255)
                                   ccTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGctct                                                                                                                   <  1:1233273/150‑1 (MQ=255)
                                         tGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCtt                                                                                                             <  1:251887/150‑1 (MQ=255)
                                          gggAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCttt                                                                                                            <  2:3117597/150‑1 (MQ=255)
                                          gggAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCttt                                                                                                            <  1:985960/150‑1 (MQ=255)
                                          gggAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCttt                                                                                                            <  1:5194469/150‑1 (MQ=255)
                                            gAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTAt                                                                                                          <  2:1795469/150‑1 (MQ=255)
                                            gAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTAt                                                                                                          <  1:2696376/150‑1 (MQ=255)
                                               tATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGAc                                                                                                       >  2:1511909/1‑150 (MQ=255)
                                                 tttCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACAt                                                                                                     >  1:2245847/1‑150 (MQ=255)
                                                   tCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACAttt                                                                                                   <  2:1546060/150‑1 (MQ=255)
                                                   tCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAAATTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACAttt                                                                                                   <  2:1268936/150‑1 (MQ=255)
                                                       gCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCgt                                                                                               >  1:2357154/1‑150 (MQ=255)
                                                         tAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCgttg                                                                                             >  1:476685/1‑150 (MQ=255)
                                                         tAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTAACAACACCACAACCACAGGCAGTATGATTAATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCgttg                                                                                             >  2:3767497/1‑150 (MQ=255)
                                                            aaTTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtc                                                                                          >  2:2467697/1‑150 (MQ=255)
                                                            aaTTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtc                                                                                          <  2:2773782/150‑1 (MQ=255)
                                                             aTTGATTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACCTCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTAAGACATATGCGTGGTtcg                                                                                         >  2:34012/1‑150 (MQ=255)
                                                               tGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCTCATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtcgtc                                                                                       <  2:2379138/150‑1 (MQ=255)
                                                               tGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtcgtc                                                                                       >  1:5027289/1‑150 (MQ=255)
                                                               tGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtcgtc                                                                                       <  1:15873/150‑1 (MQ=255)
                                                                gTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTtcgtcg                                                                                      <  1:3404275/150‑1 (MQ=255)
                                                                     aaTATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCa                                                                                 <  2:413103/150‑1 (MQ=255)
                                                                      atatACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAg                                                                                <  2:4793211/150‑1 (MQ=255)
                                                                        ataCCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGt                                                                              >  2:1186364/1‑150 (MQ=255)
                                                                        ataCCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGt                                                                              >  1:1905434/1‑150 (MQ=255)
                                                                          aCCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTaa                                                                            >  2:780631/1‑150 (MQ=255)
                                                                          aCCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTaa                                                                            >  1:210922/1‑150 (MQ=255)
                                                                             gCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACat                                                                         <  1:5183033/150‑1 (MQ=255)
                                                                               tGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGAGTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACatta                                                                       >  1:4203179/1‑150 (MQ=255)
                                                                                      tcCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTccc                                                                <  2:1357853/150‑1 (MQ=255)
                                                                                      tcCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTccc                                                                >  2:528140/1‑150 (MQ=255)
                                                                                      tcCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTccc                                                                <  2:3345362/150‑1 (MQ=255)
                                                                                       ccccAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCt                                                               <  2:4119657/150‑1 (MQ=255)
                                                                                        cccAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTc                                                              <  1:1326959/150‑1 (MQ=255)
                                                                                         ccAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTcc                                                             <  2:4001798/150‑1 (MQ=255)
                                                                                          cAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCa                                                            >  1:2248637/1‑150 (MQ=255)
                                                                                          cAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCa                                                            <  2:574294/150‑1 (MQ=255)
                                                                                          cAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCa                                                            >  1:3472530/1‑150 (MQ=255)
                                                                                            gggATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAAt                                                          >  2:2654915/1‑150 (MQ=255)
                                                                                               aTTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGt                                                       >  2:3628061/1‑150 (MQ=255)
                                                                                               aTTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGt                                                       >  1:2881670/1‑150 (MQ=255)
                                                                                                 tGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCg                                                     <  1:3820038/150‑1 (MQ=255)
                                                                                                  ggCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCgg                                                    <  1:579470/150‑1 (MQ=255)
                                                                                                     acaAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCtt                                                 <  2:1077594/150‑1 (MQ=255)
                                                                                                      caAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTa                                                >  2:1024275/1‑150 (MQ=255)
                                                                                                       aaaaaTTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAc                                               <  1:3695126/150‑1 (MQ=255)
                                                                                                            tttAACGTTACCACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAcaggca                                          <  2:1665773/150‑1 (MQ=255)
                                                                                                              tAACGTTACAACACCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAcaggcagg                                        <  1:2798773/150‑1 (MQ=255)
                                                                                                              tAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTAcaggcagg                                        >  1:217415/1‑150 (MQ=255)
                                                                                                               aaCGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACaggcaggc                                       <  2:1958898/150‑1 (MQ=255)
                                                                                                               aaCGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACaggcaggc                                       >  2:5198199/1‑150 (MQ=255)
                                                                                                               aaCGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACaggcaggc                                       >  1:1743085/1‑150 (MQ=255)
                                                                                                                    tACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAaca                                  >  2:3077124/1‑150 (MQ=255)
                                                                                                                     acaacaCCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAacac                                 <  2:1609327/150‑1 (MQ=255)
                                                                                                                     acaacaCCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAacac                                 <  1:4415648/150‑1 (MQ=255)
                                                                                                                       aacaCCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTACCATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGt                               >  2:4441358/1‑150 (MQ=255)
                                                                                                                        acacCACATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTc                              <  2:2248582/150‑1 (MQ=255)
                                                                                                                          accacATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTg                            >  2:4850529/1‑150 (MQ=255)
                                                                                                                           ccacATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTgg                           <  1:4507556/150‑1 (MQ=255)
                                                                                                                           ccacATCCACAGGCAGTATGATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTgg                           <  1:295316/150‑1 (MQ=255)
                                                                                                                              caTCAACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATTACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACt                        >  1:3534570/1‑150 (MQ=255)
                                                                                                                               aTCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTg                       >  2:1134281/1‑150 (MQ=255)
                                                                                                                               aTCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTg                       >  2:3805417/1‑150 (MQ=255)
                                                                                                                                 ccACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCg                     <  1:528140/150‑1 (MQ=255)
                                                                                                                                 ccACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCg                     <  2:1006450/150‑1 (MQ=255)
                                                                                                                                      ggCAGTATGATTTATGACCCATCAATTAAGATCGCGCTATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCAtt                <  1:3173031/150‑1 (MQ=255)
                                                                                                                                       tcAGTATTATTTATTACCCATCAATTAAGATCGCGCGATATCATCGCTCTGTGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGATTACAGGCAGGCGAACACGTCTGGACTGCGGCATTc               <  2:4150107/149‑1 (MQ=255)
                                                                                                                                       gCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTc               <  1:2290513/150‑1 (MQ=255)
                                                                                                                                       gCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTc               <  2:3367227/150‑1 (MQ=255)
                                                                                                                                       gCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTc               <  1:4622727/150‑1 (MQ=255)
                                                                                                                                            aTGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCtt          >  1:3571938/1‑150 (MQ=255)
                                                                                                                                            aTGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCtt          <  1:4342762/150‑1 (MQ=255)
                                                                                                                                             tGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTc         <  2:1904478/150‑1 (MQ=255)
                                                                                                                                               aTTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCt       >  2:4269268/1‑150 (MQ=255)
                                                                                                                                               aTTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCt       >  1:1053342/1‑150 (MQ=255)
                                                                                                                                               aTTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGGTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCt       <  2:721392/150‑1 (MQ=255)
                                                                                                                                                 ttATGACCAATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACAATATTTTCCCTCAAATGGTCGGCTTACATGCAGGCGAACACGTCTGGACTGCGGCATACGGCTTCCTCa     >  2:2036755/1‑150 (MQ=255)
                                                                                                                                                  tATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCAt    <  1:2470264/150‑1 (MQ=255)
                                                                                                                                                    tGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTa  <  2:4363545/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TAACGCGGTAAATCGAAAAACTATTCTTCGCCGCGCCTGGTTGGGAGTATTTCCCGCTAAAATTGTTTAAATATACCGCTGTATCATCCCCAGGGATTGGCACAAAAATTTAACGTTACAACACCACATCCACAGGCAGTATGATTTATGACCCATCAATTAAGATCGCGCGATATCATCGCTCTGGGCTTTATGACATTTGCGTTGTTCGTCGGCGCAGGTAACATTATTTTCCCTCCAATGGTCGGCTTACAGGCAGGCGAACACGTCTGGACTGCGGCATTCGGCTTCCTCATTA  >  CP009273/414900‑415197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A