Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 430,523 G→A A141T (GCA→ACA)  ribE → riboflavin synthase beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273430,5230GA99.5% 632.4 / NA 210A141T (GCA→ACA) ribEriboflavin synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (126/83);  minor base C (1/0);  total (127/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

ATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACG  >  CP009273/430377‑430668
                                                                                                                                                  |                                                                                                                                                 
atGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTacac                                                                                                                                                >  1:2600589/1‑150 (MQ=255)
 tGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACt                                                                                                                                               >  1:3546423/1‑150 (MQ=255)
 tGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACt                                                                                                                                               >  1:1554108/1‑150 (MQ=255)
  gTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTg                                                                                                                                              >  1:1311521/1‑150 (MQ=255)
  gTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTg                                                                                                                                              <  2:2735448/150‑1 (MQ=255)
    cGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGAc                                                                                                                                            <  2:716147/150‑1 (MQ=255)
     gCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGAcc                                                                                                                                           <  1:1310706/150‑1 (MQ=255)
      cTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACcg                                                                                                                                          >  2:2549494/1‑150 (MQ=255)
       tggtggTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACcgc                                                                                                                                         >  2:1068405/1‑150 (MQ=255)
              gcaagcaaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGaa                                                                                                                                  >  2:645707/1‑150 (MQ=255)
              gcaagcaaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGaa                                                                                                                                  <  2:2227055/150‑1 (MQ=255)
                aagcaaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAAt                                                                                                                                <  2:808213/150‑1 (MQ=255)
                 agcaaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATg                                                                                                                               >  1:2798842/1‑150 (MQ=255)
                 agcaaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGGTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATg                                                                                                                               <  2:1800320/150‑1 (MQ=255)
                   caaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGAt                                                                                                                             >  2:2793183/1‑150 (MQ=255)
                   caaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGAt                                                                                                                             >  2:2887445/1‑150 (MQ=255)
                   caaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGAt                                                                                                                             >  2:1224663/1‑150 (MQ=255)
                    aaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGAtt                                                                                                                            >  2:3005619/1‑150 (MQ=255)
                    aaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGAtt                                                                                                                            >  2:195590/1‑150 (MQ=255)
                    aaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGAtt                                                                                                                            >  1:831084/1‑150 (MQ=255)
                     aCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGTCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTa                                                                                                                           <  1:2571471/150‑1 (MQ=255)
                     aCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTa                                                                                                                           <  2:3277847/150‑1 (MQ=255)
                      cGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTaa                                                                                                                          <  1:1016929/150‑1 (MQ=255)
                       ggCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAAt                                                                                                                         <  1:1695222/150‑1 (MQ=255)
                       ggCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAAt                                                                                                                         <  1:2617845/150‑1 (MQ=255)
                          cTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTa                                                                                                                      >  2:2388318/1‑150 (MQ=255)
                           tGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTAt                                                                                                                     <  2:1680867/150‑1 (MQ=255)
                           tGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTAt                                                                                                                     <  1:2754811/150‑1 (MQ=255)
                             gcgcATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTg                                                                                                                   <  2:3529743/150‑1 (MQ=255)
                              cgcATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGa                                                                                                                  <  1:957978/150‑1 (MQ=255)
                                cATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGaaa                                                                                                                >  2:835136/1‑150 (MQ=255)
                                 aTGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAg                                                                                                               >  2:3100267/1‑150 (MQ=255)
                                     tGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCAt                                                                                                           <  1:1234809/150‑1 (MQ=255)
                                          aGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAgg                                                                                                      <  2:3038831/150‑1 (MQ=255)
                                           ggACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGc                                                                                                     >  1:1566378/1‑150 (MQ=255)
                                            gACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGcc                                                                                                    >  2:1826675/1‑150 (MQ=255)
                                            gACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGcc                                                                                                    <  2:681941/150‑1 (MQ=255)
                                             aCAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCt                                                                                                   <  1:1386137/150‑1 (MQ=255)
                                             aCAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCt                                                                                                   <  1:2116279/150‑1 (MQ=255)
                                               aGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGa                                                                                                 >  1:1477317/1‑150 (MQ=255)
                                                   aaaTTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAAtt                                                                                             <  2:481293/150‑1 (MQ=255)
                                                     aTTCCGTTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAg                                                                                           <  1:1574265/150‑1 (MQ=255)
                                                       tCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTa                                                                                         >  2:3789745/1‑150 (MQ=255)
                                                        ccGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTaa                                                                                        >  2:759636/1‑150 (MQ=255)
                                                         cGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAg                                                                                       <  2:1925709/150‑1 (MQ=255)
                                                          ggTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAgg                                                                                      <  2:251691/150‑1 (MQ=255)
                                                           gTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAggg                                                                                     <  2:2954947/150‑1 (MQ=255)
                                                               gtggtGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGCAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTATGGGGaaa                                                                                 <  2:3715414/146‑1 (MQ=255)
                                                               cTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGaaa                                                                                 <  2:276735/150‑1 (MQ=255)
                                                                ttttGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAAt                                                                                <  2:3347783/150‑1 (MQ=255)
                                                                         tCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAc                                                                       >  2:262006/1‑150 (MQ=255)
                                                                          cTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAcc                                                                      <  2:1945816/150‑1 (MQ=255)
                                                                           tGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAGGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACct                                                                     >  1:2719723/1‑150 (MQ=255)
                                                                           tGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACct                                                                     <  1:3212196/150‑1 (MQ=255)
                                                                           tGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACct                                                                     >  1:2516662/1‑150 (MQ=255)
                                                                            gACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACctg                                                                    <  2:160245/150‑1 (MQ=255)
                                                                               cACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACctgctg                                                                 <  1:663076/150‑1 (MQ=255)
                                                                                 cTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTgctgct                                                               >  1:2351193/1‑150 (MQ=255)
                                                                                 cTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTgctgct                                                               >  1:445792/1‑150 (MQ=255)
                                                                                  tGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCtc                                                              >  2:3197473/1‑150 (MQ=255)
                                                                                  tGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCtc                                                              >  2:3407834/1‑150 (MQ=255)
                                                                                   gAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCtcg                                                             >  1:2505699/1‑150 (MQ=255)
                                                                                      aGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCtcgtcg                                                          >  1:1228074/1‑150 (MQ=255)
                                                                                       gCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTcgc                                                         >  1:2310622/1‑150 (MQ=255)
                                                                                       gCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTcgc                                                         >  1:2476481/1‑150 (MQ=255)
                                                                                        cATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTcgcc                                                        <  1:1303744/150‑1 (MQ=255)
                                                                                         aTTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTcgccg                                                       >  1:50164/1‑150 (MQ=255)
                                                                                           tGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCcgcg                                                     >  1:2166805/1‑150 (MQ=255)
                                                                                            gAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCgcgc                                                    >  1:1928718/1‑150 (MQ=255)
                                                                                            gAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCgcgc                                                    >  1:268575/1‑150 (MQ=255)
                                                                                                 aGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTg                                               >  2:3167082/1‑150 (MQ=255)
                                                                                                  gCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGa                                              >  1:2412108/1‑150 (MQ=255)
                                                                                                  gCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGa                                              >  1:1146194/1‑150 (MQ=255)
                                                                                                    gATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAgt                                            >  2:1409787/1‑150 (MQ=255)
                                                                                                        gAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGc                                        >  2:3047712/1‑150 (MQ=255)
                                                                                                         aaCGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGcc                                       >  2:2300431/1‑150 (MQ=255)
                                                                                                         aaCGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGcc                                       <  1:3447597/150‑1 (MQ=255)
                                                                                                              gCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCa                                  <  2:3788795/150‑1 (MQ=255)
                                                                                                                    aCCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGct                            >  1:2860785/1‑150 (MQ=255)
                                                                                                                    aCCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGct                            <  2:1566378/150‑1 (MQ=255)
                                                                                                                    aCCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGct                            <  1:54527/150‑1 (MQ=255)
                                                                                                                      cAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGctct                          >  1:3096938/1‑150 (MQ=255)
                                                                                                                        aaGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTAc                        >  1:388270/1‑150 (MQ=255)
                                                                                                                         aGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACt                       >  2:3126851/1‑150 (MQ=255)
                                                                                                                           cTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACATGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTcc                     >  1:2014858/1‑150 (MQ=255)
                                                                                                                            tGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCt                    >  1:431095/1‑150 (MQ=255)
                                                                                                                            tGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCt                    <  2:1519863/150‑1 (MQ=255)
                                                                                                                              gCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTgg                  >  1:2699308/1‑150 (MQ=255)
                                                                                                                              gCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTgg                  >  1:3566242/1‑150 (MQ=255)
                                                                                                                                aacaaAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCa                >  1:3680942/1‑150 (MQ=255)
                                                                                                                                    aaGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTg            >  2:2065742/1‑150 (MQ=255)
                                                                                                                                     aGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGt           >  1:1071475/1‑150 (MQ=255)
                                                                                                                                        tGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTccc        >  2:416984/1‑150 (MQ=255)
                                                                                                                                          cAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAg      >  2:2208881/1‑150 (MQ=255)
                                                                                                                                          cAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAg      >  1:2230027/1‑150 (MQ=255)
                                                                                                                                          cAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAg      >  1:3704698/1‑150 (MQ=255)
                                                                                                                                          cAGAAGCTACACTGACCGCGCGTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCAACTCCTGGCAGTTGTCCCAg      >  1:3397760/1‑150 (MQ=255)
                                                                                                                                           aGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGa     >  2:3574215/1‑150 (MQ=255)
                                                                                                                                            gAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGaa    >  2:2260179/1‑150 (MQ=255)
                                                                                                                                             aaGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAAc   >  2:2217278/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACG  >  CP009273/430377‑430668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAAC  >  CP009273/430374‑430667
                                                                                                                                                     |                                                                                                                                                
AATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTA                                                                                                                                                  >  SRR19973061.2032137/1‑150 (MQ=60)
 ATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTAC                                                                                                                                                 >  SRR19973061.3813562/1‑150 (MQ=60)
   ATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACAC                                                                                                                                               >  SRR19973061.2607267/1‑150 (MQ=60)
   ATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACAC                                                                                                                                               >  SRR19973061.2197331/1‑150 (MQ=60)
    TGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACT                                                                                                                                              >  SRR19973061.1557969/1‑150 (MQ=60)
    TGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACT                                                                                                                                              >  SRR19973061.3555506/1‑150 (MQ=60)
     GTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTG                                                                                                                                             >  SRR19973061.1314807/1‑150 (MQ=60)
     GTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTG                                                                                                                                             >  SRR19973061.1314031/1‑150 (MQ=60)
        GCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACC                                                                                                                                          <  SRR19973061.1313988/150‑1 (MQ=60)
           GGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCG                                                                                                                                       <  SRR19973061.1505506/150‑1 (MQ=60)
                  CAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAA                                                                                                                                >  SRR19973061.866710/1‑150 (MQ=60)
                    AGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATG                                                                                                                              >  SRR19973061.2806035/1‑150 (MQ=60)
                     GCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGA                                                                                                                             <  SRR19973061.2102359/150‑1 (MQ=60)
                       AACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATT                                                                                                                           >  SRR19973061.833255/1‑150 (MQ=60)
                        ACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGTCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTA                                                                                                                          <  SRR19973061.2578051/150‑1 (MQ=60)
                        ACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTA                                                                                                                          <  SRR19973061.1406042/150‑1 (MQ=60)
                        ACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTA                                                                                                                          <  SRR19973061.1406087/150‑1 (MQ=60)
                         CGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAA                                                                                                                         <  SRR19973061.1019519/150‑1 (MQ=60)
                          GGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAAT                                                                                                                        <  SRR19973061.2624566/150‑1 (MQ=60)
                          GGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAAT                                                                                                                        <  SRR19973061.1699479/150‑1 (MQ=60)
                          GGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAAT                                                                                                                        >  SRR19973061.2541063/1‑150 (MQ=60)
                            CCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGT                                                                                                                      <  SRR19973061.2800359/150‑1 (MQ=60)
                              TGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTAT                                                                                                                    <  SRR19973061.2761906/150‑1 (MQ=60)
                                 CGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGA                                                                                                                 <  SRR19973061.960394/150‑1 (MQ=60)
                                        TGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCAT                                                                                                          <  SRR19973061.1237877/150‑1 (MQ=60)
                                        TGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCAT                                                                                                          >  SRR19973061.3359759/1‑150 (MQ=60)
                                         GCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATC                                                                                                         <  SRR19973061.1312896/150‑1 (MQ=60)
                                             AGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGG                                                                                                     >  SRR19973061.1340081/1‑150 (MQ=60)
                                              GGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCCAGGC                                                                                                    >  SRR19973061.1570267/1‑150 (MQ=60)
                                              GGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGC                                                                                                    >  SRR19973061.1570232/1‑150 (MQ=60)
                                                ACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCT                                                                                                  <  SRR19973061.1389648/150‑1 (MQ=60)
                                                ACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCT                                                                                                  <  SRR19973061.2121594/150‑1 (MQ=60)
                                                ACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCT                                                                                                  <  SRR19973061.2248405/150‑1 (MQ=60)
                                                  AGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGA                                                                                                >  SRR19973061.700889/1‑150 (MQ=60)
                                                  AGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGA                                                                                                >  SRR19973061.1481027/1‑150 (MQ=60)
                                                     GAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAAT                                                                                             >  SRR19973061.2317686/1‑150 (MQ=60)
                                                      AAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCCAGGCCTGAAATT                                                                                            >  SRR19973061.508250/1‑150 (MQ=60)
                                                        ATTCCGTTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAG                                                                                          <  SRR19973061.1578177/150‑1 (MQ=60)
                                                        ATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAG                                                                                          <  SRR19973061.1578142/150‑1 (MQ=60)
                                                         TTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGT                                                                                         <  SRR19973061.3761473/150‑1 (MQ=60)
                                                             GGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGG                                                                                     >  SRR19973061.3615874/1‑150 (MQ=60)
                                                             GGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGG                                                                                     >  SRR19973061.426953/1‑150 (MQ=60)
                                                                      TGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCG                                                                            <  SRR19973061.2123664/150‑1 (MQ=60)
                                                                       GGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGT                                                                           >  SRR19973061.3449169/1‑150 (MQ=60)
                                                                            TCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAC                                                                      >  SRR19973061.1064201/1‑150 (MQ=60)
                                                                              TGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAGGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCT                                                                    >  SRR19973061.2726722/1‑150 (MQ=60)
                                                                              TGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAGGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATAAGTAAGGGGAAATCCGTGAAACCT                                                                    >  SRR19973061.2726718/1‑150 (MQ=60)
                                                                              TGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCT                                                                    <  SRR19973061.3220448/150‑1 (MQ=60)
                                                                              TGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCT                                                                    >  SRR19973061.2522841/1‑150 (MQ=60)
                                                                              TGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCT                                                                    >  SRR19973061.2523077/1‑150 (MQ=60)
                                                                              TGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCT                                                                    >  SRR19973061.2523079/1‑150 (MQ=60)
                                                                                 CCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCT                                                                 <  SRR19973061.1138659/150‑1 (MQ=60)
                                                                                  CACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTG                                                                <  SRR19973061.3800276/150‑1 (MQ=60)
                                                                                  CACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTG                                                                <  SRR19973061.664782/150‑1 (MQ=60)
                                                                                    CTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCT                                                              >  SRR19973061.2357169/1‑150 (MQ=60)
                                                                                    CTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCT                                                              >  SRR19973061.447002/1‑150 (MQ=60)
                                                                                      GAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCG                                                            >  SRR19973061.2512077/1‑150 (MQ=60)
                                                                                        AAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTC                                                          <  SRR19973061.1935262/150‑1 (MQ=60)
                                                                                         AGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCG                                                         >  SRR19973061.1231133/1‑150 (MQ=60)
                                                                                         AGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCG                                                         >  SRR19973061.1231139/1‑150 (MQ=60)
                                                                                          GCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGC                                                        >  SRR19973061.2316481/1‑150 (MQ=60)
                                                                                          GCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGC                                                        >  SRR19973061.2482787/1‑150 (MQ=60)
                                                                                          GCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGC                                                        >  SRR19973061.2482786/1‑150 (MQ=60)
                                                                                           CATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCC                                                       >  SRR19973061.1208608/1‑150 (MQ=60)
                                                                                           CATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCC                                                       <  SRR19973061.1306998/150‑1 (MQ=60)
                                                                                            ATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCG                                                      >  SRR19973061.50346/1‑150 (MQ=60)
                                                                                            ATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCG                                                      >  SRR19973061.50360/1‑150 (MQ=60)
                                                                                              TGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCG                                                    >  SRR19973061.2172244/1‑150 (MQ=60)
                                                                                               GAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGC                                                   >  SRR19973061.1933509/1‑150 (MQ=60)
                                                                                               GAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGC                                                   >  SRR19973061.269193/1‑150 (MQ=60)
                                                                                               GAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGC                                                   >  SRR19973061.269312/1‑150 (MQ=60)
                                                                                                 ACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTC                                                 <  SRR19973061.596561/150‑1 (MQ=60)
                                                                                                  CAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCG                                                <  SRR19973061.3050506/150‑1 (MQ=60)
                                                                                                  CAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAAAGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCG                                                <  SRR19973061.3050723/150‑1 (MQ=60)
                                                                                                   AAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGT                                               >  SRR19973061.1686908/1‑150 (MQ=60)
                                                                                                     GCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGA                                             >  SRR19973061.2418266/1‑150 (MQ=60)
                                                                                                     GCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGA                                             >  SRR19973061.1149109/1‑150 (MQ=60)
                                                                                                           GAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGC                                       >  SRR19973061.2581536/1‑150 (MQ=60)
                                                                                                            AACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCC                                      <  SRR19973061.3456446/150‑1 (MQ=60)
                                                                                                                  CTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAG                                >  SRR19973061.1262658/1‑150 (MQ=60)
                                                                                                                      CACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGC                            >  SRR19973061.235850/1‑150 (MQ=60)
                                                                                                                      CACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGC                            >  SRR19973061.3692587/1‑150 (MQ=60)
                                                                                                                       ACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCT                           >  SRR19973061.2868111/1‑150 (MQ=60)
                                                                                                                       ACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCT                           >  SRR19973061.2865156/1‑150 (MQ=60)
                                                                                                                       ACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCT                           <  SRR19973061.54735/150‑1 (MQ=60)
                                                                                                                         CAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCT                         >  SRR19973061.3104917/1‑150 (MQ=60)
                                                                                                                           AAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTAC                       >  SRR19973061.2462611/1‑150 (MQ=60)
                                                                                                                           AAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTAC                       >  SRR19973061.389331/1‑150 (MQ=60)
                                                                                                                           AAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTAC                       >  SRR19973061.3126190/1‑150 (MQ=60)
                                                                                                                              CTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACATGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCC                    >  SRR19973061.2019919/1‑150 (MQ=60)
                                                                                                                               TGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCT                   >  SRR19973061.974250/1‑150 (MQ=60)
                                                                                                                               TGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCT                   >  SRR19973061.974234/1‑150 (MQ=60)
                                                                                                                               TGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCT                   >  SRR19973061.432296/1‑150 (MQ=60)
                                                                                                                               TGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCT                   >  SRR19973061.432269/1‑150 (MQ=60)
                                                                                                                                 GCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGG                 >  SRR19973061.3575375/1‑150 (MQ=60)
                                                                                                                                 GCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGG                 >  SRR19973061.3575377/1‑150 (MQ=60)
                                                                                                                                 GCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGG                 >  SRR19973061.2706249/1‑150 (MQ=60)
                                                                                                                                 GCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGG                 >  SRR19973061.2706209/1‑150 (MQ=60)
                                                                                                                                   AACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCA               >  SRR19973061.3690348/1‑150 (MQ=60)
                                                                                                                                     CAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGT             <  SRR19973061.3463315/150‑1 (MQ=60)
                                                                                                                                        AGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGT          <  SRR19973061.1813690/150‑1 (MQ=60)
                                                                                                                                        AGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGT          >  SRR19973061.1074223/1‑150 (MQ=60)
                                                                                                                                           TGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCC       >  SRR19973061.2172238/1‑150 (MQ=60)
                                                                                                                                             CAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAG     >  SRR19973061.2235620/1‑150 (MQ=60)
                                                                                                                                             CAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAG     >  SRR19973061.3714167/1‑150 (MQ=60)
                                                                                                                                             CAGAAGCTACACTGACCGCGCGTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCAACTCCTGGCAGTTGTCCCAG     >  SRR19973061.3406481/1‑150 (MQ=60)
                                                                                                                                               GAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAA   >  SRR19973061.2618713/1‑150 (MQ=60)
                                                                                                                                                AAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAAC  <  SRR19973061.2758567/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                
AATATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAAC  >  CP009273/430374‑430667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑