Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 430,523 G→A 25.1% A141T (GCA→ACA)  ribE → riboflavin synthase beta chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273430,5230GA25.1% 633.1 / 209.5 307A141T (GCA→ACA) ribEriboflavin synthase beta chain
Reads supporting (aligned to +/- strand):  ref base G (111/119);  new base A (37/40);  total (148/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

ATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACG  >  CP009273/430377‑430668
                                                                                                                                                  |                                                                                                                                                 
 tGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACt                                                                                                                                               <  2:1555849/150‑1 (MQ=255)
  gTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTg                                                                                                                                              >  1:3403812/1‑150 (MQ=255)
   tCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGa                                                                                                                                             >  2:4608924/1‑150 (MQ=255)
    cGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGAc                                                                                                                                            <  2:4753312/150‑1 (MQ=255)
     gCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGAcc                                                                                                                                           <  1:1955679/150‑1 (MQ=255)
     gCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGAcc                                                                                                                                           <  2:231786/150‑1 (MQ=255)
      cTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACcg                                                                                                                                          <  1:2681822/150‑1 (MQ=255)
             tgCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGa                                                                                                                                   <  2:3102091/150‑1 (MQ=255)
               caagcaaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGaaa                                                                                                                                 <  1:5127879/150‑1 (MQ=255)
                aagcaaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGGCCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAAt                                                                                                                                <  1:853607/150‑1 (MQ=255)
                 agcaaCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATg                                                                                                                               >  1:33107/1‑150 (MQ=255)
                     aCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTa                                                                                                                           >  1:488047/1‑150 (MQ=255)
                     aCGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTa                                                                                                                           <  2:2236517/150‑1 (MQ=255)
                       ggCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAAt                                                                                                                         >  2:511021/1‑150 (MQ=255)
                       ggCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAAt                                                                                                                         <  1:1822911/150‑1 (MQ=255)
                        gCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATg                                                                                                                        >  2:150073/1‑150 (MQ=255)
                        gCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATg                                                                                                                        <  2:552869/150‑1 (MQ=255)
                        gCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATg                                                                                                                        <  1:2533835/150‑1 (MQ=255)
                        gCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGAGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAAtt                                                                                                                        <  1:3277238/150‑2 (MQ=255)
                             gcgcATGTGGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAACGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTg                                                                                                                   <  2:1018540/150‑1 (MQ=255)
                                   gTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGcc                                                                                                             <  2:418603/150‑1 (MQ=255)
                                      gCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATc                                                                                                          <  2:2458028/150‑1 (MQ=255)
                                         cAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAg                                                                                                       >  1:2150326/1‑150 (MQ=255)
                                          aGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAgg                                                                                                      >  1:533041/1‑150 (MQ=255)
                                           ggACAGCGAAATTCCGGTTGCTTTTGTGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGc                                                                                                     <  1:2431220/150‑1 (MQ=255)
                                           ggACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAATCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGc                                                                                                     >  1:432698/1‑150 (MQ=255)
                                           ggACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGc                                                                                                     >  1:4626802/1‑150 (MQ=255)
                                            gACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGcc                                                                                                    <  1:3551538/150‑1 (MQ=255)
                                             aCAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCt                                                                                                   <  2:1703806/150‑1 (MQ=255)
                                             aCAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCt                                                                                                   <  2:509215/150‑1 (MQ=255)
                                                  gAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAAt                                                                                              <  2:2185887/150‑1 (MQ=255)
                                                   aaaTTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAAtt                                                                                             <  1:1481809/150‑1 (MQ=255)
                                                   aaaTTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAAtt                                                                                             <  1:3308604/150‑1 (MQ=255)
                                                    aaTTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCCAGGCCTGAAATTa                                                                                            >  2:5305967/1‑150 (MQ=255)
                                                    aaTTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTa                                                                                            >  1:2050153/1‑150 (MQ=255)
                                                    aaTTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTa                                                                                            >  1:5246728/1‑150 (MQ=255)
                                                     aTTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAg                                                                                           <  1:4752753/150‑1 (MQ=255)
                                                      ttCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGt                                                                                          <  2:3558388/150‑1 (MQ=255)
                                                        ccGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTaa                                                                                        >  1:4278748/1‑150 (MQ=255)
                                                          gtttGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAgg                                                                                      <  1:5013513/148‑1 (MQ=255)
                                                             tGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGa                                                                                   >  1:1906423/1‑150 (MQ=255)
                                                             tGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGa                                                                                   >  1:2983863/1‑150 (MQ=255)
                                                               cTTGTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGTCCTGAAATTAGTAAGGGGaaa                                                                                 <  1:4559676/150‑1 (MQ=255)
                                                                  ttGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATcc                                                                              <  2:3957253/150‑1 (MQ=255)
                                                                    ggggTTCTGACCACTGAAAGCATTGAACACGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGt                                                                            <  1:3046665/150‑1 (MQ=255)
                                                                    ggggTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGt                                                                            >  1:1994141/1‑150 (MQ=255)
                                                                      ggTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGa                                                                          >  2:285872/1‑150 (MQ=255)
                                                                         tCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAc                                                                       <  1:4971041/150‑1 (MQ=255)
                                                                         tCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAc                                                                       <  1:3744911/150‑1 (MQ=255)
                                                                         tCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAc                                                                       <  2:4236179/150‑1 (MQ=255)
                                                                          cTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAcc                                                                      <  2:506716/150‑1 (MQ=255)
                                                                          cTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAcc                                                                      <  2:3249918/150‑1 (MQ=255)
                                                                          cTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAAcc                                                                      >  1:1311707/1‑150 (MQ=255)
                                                                           tGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACct                                                                     <  1:1365838/150‑1 (MQ=255)
                                                                             aCCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACctgc                                                                   >  1:2907277/1‑150 (MQ=255)
                                                                              ccACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACctgct                                                                  >  2:2086617/1‑150 (MQ=255)
                                                                                    aaaGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTCAGGGGAAATCCGTGAAACCTGCTGCtcgt                                                            >  2:3344459/1‑150 (MQ=255)
                                                                                    aaaGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCtcgt                                                            >  1:4426759/1‑150 (MQ=255)
                                                                                    aaaGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCTAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCtcgt                                                            <  1:3984078/150‑1 (MQ=255)
                                                                                     aaGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCtcgtc                                                           <  1:2110956/150‑1 (MQ=255)
                                                                                     aaGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCtcgtc                                                           >  2:2786310/1‑150 (MQ=255)
                                                                                        cATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTcgcc                                                        >  1:3206097/1‑150 (MQ=255)
                                                                                         aTTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTcgccg                                                       >  2:4950623/1‑150 (MQ=255)
                                                                                         aTTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTcgccg                                                       >  1:681507/1‑150 (MQ=255)
                                                                                          ttGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTcgccgc                                                      >  2:1836386/1‑150 (MQ=255)
                                                                                             aaCAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCt                                                   <  1:3940326/150‑1 (MQ=255)
                                                                                                aaGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGt                                                <  1:446566/150‑1 (MQ=255)
                                                                                                aaGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAAGTAGTCAGGGGAAATCCGTGAAACCTGCTGCTCGGCGCCGCGCTCGt                                                >  1:1001939/1‑150 (MQ=255)
                                                                                                 aGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTg                                               >  1:4045011/1‑150 (MQ=255)
                                                                                                    gATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAgt                                            >  2:2582321/1‑150 (MQ=255)
                                                                                                    gATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAgt                                            >  1:4192852/1‑150 (MQ=255)
                                                                                                      tCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAgtgt                                          <  2:5198355/150‑1 (MQ=255)
                                                                                                       cGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGtgtg                                         <  2:3468447/150‑1 (MQ=255)
                                                                                                       cGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGtgtg                                         >  2:1441766/1‑150 (MQ=255)
                                                                                                       cGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGtgtg                                         <  1:120451/150‑1 (MQ=255)
                                                                                                        gAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGc                                        >  1:3616596/1‑150 (MQ=255)
                                                                                                         aaCGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGcc                                       >  2:2356416/1‑150 (MQ=255)
                                                                                                         aaCGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGcc                                       >  2:420896/1‑150 (MQ=255)
                                                                                                         aaCGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGcc                                       <  1:1042008/150‑1 (MQ=255)
                                                                                                            gTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTc                                    >  1:5281245/1‑150 (MQ=255)
                                                                                                             tGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTcc                                   <  1:1139594/150‑1 (MQ=255)
                                                                                                              gCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCa                                  <  2:4026852/150‑1 (MQ=255)
                                                                                                               cTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAg                                 <  2:3685244/150‑1 (MQ=255)
                                                                                                                 ggCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGgc                               >  1:2354221/1‑150 (MQ=255)
                                                                                                                  gCACCAAAGCTGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGgcg                              >  2:2541276/1‑150 (MQ=255)
                                                                                                                          gCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTCTTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTc                      <  1:1407705/150‑1 (MQ=255)
                                                                                                                           cTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTcc                     <  2:1994141/150‑1 (MQ=255)
                                                                                                                           cTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTcc                     >  2:4368546/1‑150 (MQ=255)
                                                                                                                            tGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCt                    <  2:2367267/150‑1 (MQ=255)
                                                                                                                            tGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCt                    <  1:150073/150‑1 (MQ=255)
                                                                                                                            tGGCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCt                    >  1:702307/1‑150 (MQ=255)
                                                                                                                              gCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTgg                  <  2:4780813/150‑1 (MQ=255)
                                                                                                                              gCAACAAAGGTGCAGAAGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTgg                  <  2:41373/150‑1 (MQ=255)
                                                                                                                               caacaaAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGc                 >  2:848027/1‑150 (MQ=255)
                                                                                                                                  caaAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGt              >  2:1627180/1‑150 (MQ=255)
                                                                                                                                   aaaGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGtt             >  1:5048769/1‑150 (MQ=255)
                                                                                                                                        tGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTccc        <  2:2749200/150‑1 (MQ=255)
                                                                                                                                             aaGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAAc   <  1:4186292/150‑1 (MQ=255)
                                                                                                                                             aaGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAAc   >  2:1291861/1‑150 (MQ=255)
                                                                                                                                              aGCTACACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACg  >  1:4118028/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATGTCGCTGGTGGTGCAAGCAACGGCCTGGCGCATGTTGCCCAGGACAGCGAAATTCCGGTTGCTTTTGGGGTTCTGACCACTGAAAGCATTGAACAAGCGATCGAACGTGCTGGCACCAAAGCTGGCAACAAAGGTGCAGAAGCTGCACTGACCGCGCTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACG  >  CP009273/430377‑430668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A