Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,963,622 A→T intergenic (‑1/+18) cheR ← / ← tap chemotaxis regulator, protein‑glutamate methyltransferase/methyl‑accepting protein IV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,963,6220AT99.6% 816.0 / NA 246intergenic (‑1/+18)cheR/tapchemotaxis regulator, protein‑glutamate methyltransferase/methyl‑accepting protein IV
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (113/131);  total (115/131)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCAC  >  CP009273/1963475‑1963768
                                                                                                                                                   |                                                                                                                                                  
 aTGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTgcg                                                                                                                                                 <  1:931532/150‑1 (MQ=255)
  tGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTgcgc                                                                                                                                                >  2:1943167/1‑150 (MQ=255)
  tGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTgcgc                                                                                                                                                >  1:3414075/1‑150 (MQ=255)
    tCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCt                                                                                                                                              <  1:3460765/150‑1 (MQ=255)
     cgcgTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCtt                                                                                                                                             <  1:3196480/150‑1 (MQ=255)
        gTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTtctc                                                                                                                                          >  2:1307086/1‑150 (MQ=255)
        gTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTtctc                                                                                                                                          >  2:3253257/1‑150 (MQ=255)
         tttATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCa                                                                                                                                         <  1:261045/150‑1 (MQ=255)
           tATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAAt                                                                                                                                       >  2:1523899/1‑150 (MQ=255)
               gTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACt                                                                                                                                   >  1:2916952/1‑150 (MQ=255)
                tCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACtt                                                                                                                                  >  2:1010746/1‑150 (MQ=255)
                tCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGAAGAGATGAAGTCATTGCGCCTTCTCAATCACtt                                                                                                                                  >  2:594996/1‑150 (MQ=255)
                 cAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTc                                                                                                                                 <  1:3373522/150‑1 (MQ=255)
                 cAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTc                                                                                                                                 <  1:3793370/150‑1 (MQ=255)
                     cAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGa                                                                                                                             <  1:976035/150‑1 (MQ=255)
                       gAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATa                                                                                                                           >  1:873935/1‑150 (MQ=255)
                          cGATCCCGGCTCGTTGATTGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCa                                                                                                                        <  1:3677935/150‑1 (MQ=255)
                          cGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCa                                                                                                                        >  1:1289634/1‑150 (MQ=255)
                            aTCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACt                                                                                                                      <  1:1523261/150‑1 (MQ=255)
                            aTCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACt                                                                                                                      <  1:3591499/150‑1 (MQ=255)
                            aTCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACt                                                                                                                      <  1:1523009/150‑1 (MQ=255)
                             tCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTg                                                                                                                     <  2:492995/150‑1 (MQ=255)
                                cGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGgcg                                                                                                                  <  1:1753714/150‑1 (MQ=255)
                                 ggCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGgcgc                                                                                                                 >  2:632229/1‑150 (MQ=255)
                                 ggCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGgcgc                                                                                                                 >  1:1204594/1‑150 (MQ=255)
                                   cTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCaa                                                                                                               >  1:3728513/1‑150 (MQ=255)
                                   cTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCaa                                                                                                               <  2:2671971/150‑1 (MQ=255)
                                    tCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAAt                                                                                                              >  2:2985717/1‑150 (MQ=255)
                                          aTAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTaa                                                                                                        >  1:1104170/1‑150 (MQ=255)
                                           tAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAAc                                                                                                       >  1:1161514/1‑150 (MQ=255)
                                            aGATCAATTGACTTATCCGCCGAAAATGCGCGCCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACt                                                                                                      <  1:1138867/150‑1 (MQ=255)
                                             gATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTg                                                                                                     >  2:3431188/1‑150 (MQ=255)
                                               tCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCa                                                                                                   <  2:2514567/150‑1 (MQ=255)
                                                cAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCAc                                                                                                  >  1:1447324/1‑150 (MQ=255)
                                                  aTTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCg                                                                                                >  1:1711299/1‑150 (MQ=255)
                                                     gACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACt                                                                                             <  1:2372422/150‑1 (MQ=255)
                                                      aCTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTc                                                                                            >  2:1266549/1‑150 (MQ=255)
                                                           tCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTc                                                                                       <  2:723266/150‑1 (MQ=255)
                                                           tCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTc                                                                                       <  2:45052/150‑1 (MQ=255)
                                                            ccgccgAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCg                                                                                      >  2:3355280/1‑150 (MQ=255)
                                                               ccgAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGc                                                                                   <  2:383706/150‑1 (MQ=255)
                                                                cgAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCt                                                                                  >  1:202611/1‑150 (MQ=255)
                                                                 gAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTa                                                                                 <  2:74130/150‑1 (MQ=255)
                                                                 gAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTa                                                                                 >  2:1464382/1‑150 (MQ=255)
                                                                 gAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTa                                                                                 <  1:1545880/150‑1 (MQ=255)
                                                                  aaaaTGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTAc                                                                                <  1:3417162/150‑1 (MQ=255)
                                                                    aaTGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACtt                                                                              >  1:3260968/1‑150 (MQ=255)
                                                                           gTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttc                                                                       >  2:1531272/1‑150 (MQ=255)
                                                                           gTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttc                                                                       <  1:3381682/150‑1 (MQ=255)
                                                                              ggAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttcttc                                                                    >  2:312273/1‑150 (MQ=255)
                                                                              ggAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttcttc                                                                    >  2:3629762/1‑150 (MQ=255)
                                                                              ggAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttcttc                                                                    >  2:1443448/1‑150 (MQ=255)
                                                                              ggAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttcttc                                                                    <  2:2708782/150‑1 (MQ=255)
                                                                               gAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGAAGAGATGAAGACATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCAACTCATGTCGTGCTACTACATGTTCTTCa                                                                   >  1:224771/1‑150 (MQ=255)
                                                                                 aaGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAg                                                                 >  2:2553488/1‑150 (MQ=255)
                                                                                   gcgcCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAggg                                                               <  2:913537/150‑1 (MQ=255)
                                                                                      ccAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTaa                                                            <  1:139197/150‑1 (MQ=255)
                                                                                      ccAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTaa                                                            >  2:2575808/1‑150 (MQ=255)
                                                                                        aGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAAt                                                          <  2:1093653/150‑1 (MQ=255)
                                                                                           cgcTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATAcc                                                       <  1:3009744/150‑1 (MQ=255)
                                                                                             cTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGc                                                     >  1:1040615/1‑150 (MQ=255)
                                                                                              tCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGcc                                                    <  2:1104170/150‑1 (MQ=255)
                                                                                               cGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCca                                                   >  2:1090303/1‑150 (MQ=255)
                                                                                                ggTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCcac                                                  <  2:977084/150‑1 (MQ=255)
                                                                                                ggTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCcac                                                  >  2:3216310/1‑150 (MQ=255)
                                                                                                 gTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCcaca                                                 >  2:1600587/1‑150 (MQ=255)
                                                                                                 gTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCcaca                                                 >  2:2809788/1‑150 (MQ=255)
                                                                                                       tGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcgac                                           <  2:2917075/150‑1 (MQ=255)
                                                                                                        gTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcgacg                                          <  2:3737693/150‑1 (MQ=255)
                                                                                                         tAACAATAAAGACGTTTGCCCACAGGGCAGAGATGATGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcgacga                                         <  1:59968/150‑1 (MQ=255)
                                                                                                         tAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcgacga                                         <  1:1450771/150‑1 (MQ=255)
                                                                                                         tAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcgacga                                         <  1:1971274/150‑1 (MQ=255)
                                                                                                             aaTAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGa                                     <  2:1730983/150‑1 (MQ=255)
                                                                                                             aaTAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGa                                     <  2:3704002/150‑1 (MQ=255)
                                                                                                              aTAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGAt                                    <  2:1420164/150‑1 (MQ=255)
                                                                                                               tAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATg                                   >  2:3152877/1‑150 (MQ=255)
                                                                                                                aaaGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATgg                                  <  1:911975/150‑1 (MQ=255)
                                                                                                                aaaGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATgg                                  <  1:2720432/150‑1 (MQ=255)
                                                                                                                aaaGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATgg                                  <  2:3474182/150‑1 (MQ=255)
                                                                                                                 aaGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGt                                 >  2:1686424/1‑150 (MQ=255)
                                                                                                                  aGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTc                                <  1:576783/150‑1 (MQ=255)
                                                                                                                    aCGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCgg                              <  2:1576894/150‑1 (MQ=255)
                                                                                                                     cGTTTGCCCACCGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGc                             <  1:2153067/150‑1 (MQ=255)
                                                                                                                       tttGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCt                           <  1:591463/150‑1 (MQ=255)
                                                                                                                           cccACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGAtt                       <  2:2434072/150‑1 (MQ=255)
                                                                                                                             cacaGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCg                     >  1:2627425/1‑150 (MQ=255)
                                                                                                                             cacaGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCg                     >  2:2649707/1‑150 (MQ=255)
                                                                                                                             cacaGGGCAGAGATGAAGTCATCGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCg                     >  2:1403348/1‑150 (MQ=255)
                                                                                                                               caGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGcc                   >  2:2239298/1‑150 (MQ=255)
                                                                                                                                 tggCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAg                 >  1:2907670/2‑150 (MQ=255)
                                                                                                                                   gCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgtt               <  1:3253257/150‑1 (MQ=255)
                                                                                                                                    cAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGAAACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttg              >  2:228191/1‑150 (MQ=255)
                                                                                                                                     agagATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttgt             >  1:1939423/1‑150 (MQ=255)
                                                                                                                                       agaTGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTc           <  2:1346653/150‑1 (MQ=255)
                                                                                                                                        gaTGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTcc          >  1:722960/1‑150 (MQ=255)
                                                                                                                                         aTGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCg         <  1:1307086/150‑1 (MQ=255)
                                                                                                                                          tGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGt        >  2:1810979/1‑150 (MQ=255)
                                                                                                                                          tGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGt        >  1:1233943/1‑150 (MQ=255)
                                                                                                                                           gAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGtt       >  2:3746239/1‑150 (MQ=255)
                                                                                                                                              gTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGcc    <  1:863929/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCAC  >  CP009273/1963475‑1963768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCAC  >  CP009273/1963476‑1963768
                                                                                                                                                  |                                                                                                                                                  
ATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCG                                                                                                                                                 <  SRR19973061.932556/150‑1 (MQ=60)
ATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCG                                                                                                                                                 <  SRR19973061.933908/150‑1 (MQ=60)
 TGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGC                                                                                                                                                >  SRR19973061.3422840/1‑150 (MQ=60)
 TGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGC                                                                                                                                                >  SRR19973061.811083/1‑150 (MQ=60)
   TCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCT                                                                                                                                              <  SRR19973061.3469637/150‑1 (MQ=60)
    CGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTT                                                                                                                                             <  SRR19973061.3204682/150‑1 (MQ=60)
    CGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTT                                                                                                                                             <  SRR19973061.1299420/150‑1 (MQ=60)
    CGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTT                                                                                                                                             >  SRR19973061.1947220/1‑150 (MQ=60)
        TTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCA                                                                                                                                         <  SRR19973061.261757/150‑1 (MQ=60)
         TTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAA                                                                                                                                        >  SRR19973061.2256731/1‑150 (MQ=60)
          TATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTAGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAAT                                                                                                                                       >  SRR19973061.2485232/1‑150 (MQ=60)
           ATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATC                                                                                                                                      <  SRR19973061.119485/150‑1 (MQ=60)
              GTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACT                                                                                                                                   >  SRR19973061.2923717/1‑150 (MQ=60)
              GTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACT                                                                                                                                   >  SRR19973061.2924439/1‑150 (MQ=60)
              GTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACT                                                                                                                                   >  SRR19973061.3406430/1‑150 (MQ=60)
              GTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACT                                                                                                                                   >  SRR19973061.332402/1‑150 (MQ=60)
                CAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTC                                                                                                                                 <  SRR19973061.3803038/150‑1 (MQ=60)
                CAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTC                                                                                                                                 <  SRR19973061.3382191/150‑1 (MQ=60)
                 AGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCA                                                                                                                                <  SRR19973061.882216/150‑1 (MQ=60)
                    CAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGA                                                                                                                             <  SRR19973061.978520/150‑1 (MQ=60)
                     AGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGAT                                                                                                                            <  SRR19973061.215001/150‑1 (MQ=60)
                     AGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGAT                                                                                                                            <  SRR19973061.3452364/150‑1 (MQ=60)
                      GAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATA                                                                                                                           >  SRR19973061.876203/1‑150 (MQ=60)
                      GAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATA                                                                                                                           <  SRR19973061.965804/150‑1 (MQ=60)
                      GAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATA                                                                                                                           <  SRR19973061.965855/150‑1 (MQ=60)
                       tttgatcaCGGCTCGTTGATAGTTCTTTTGACTTATTCGCCGAAAATGCGCGTCGGTAAGTGCCAGGCGCTCGGTCATCTGTATTTATTTAGACGTTTGCCCTCTGGGCAGAGATGTAGTCATTGCGCCTTCTCAATCACTTCAGGATAC                                                                                                                          <  SRR19973061.1871486/142‑1 (MQ=60)
                         CGATCCCGGCTCGTTGATTGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCA                                                                                                                        <  SRR19973061.3687336/150‑1 (MQ=60)
                         CGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCA                                                                                                                        >  SRR19973061.1292831/1‑150 (MQ=60)
                          GATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCAC                                                                                                                       <  SRR19973061.1395662/150‑1 (MQ=60)
                           ATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACT                                                                                                                      <  SRR19973061.1526941/150‑1 (MQ=60)
                           ATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACT                                                                                                                      <  SRR19973061.1226357/150‑1 (MQ=60)
                           ATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACT                                                                                                                      <  SRR19973061.3600702/150‑1 (MQ=60)
                           ATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACT                                                                                                                      <  SRR19973061.1527065/150‑1 (MQ=60)
                           ATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACT                                                                                                                      <  SRR19973061.1526813/150‑1 (MQ=60)
                               CGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCG                                                                                                                  <  SRR19973061.1758113/150‑1 (MQ=60)
                                GGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGC                                                                                                                 >  SRR19973061.1207618/1‑150 (MQ=60)
                                  CTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAA                                                                                                               >  SRR19973061.3738047/1‑150 (MQ=60)
                                    CGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATT                                                                                                             <  SRR19973061.1982369/150‑1 (MQ=60)
                                     GTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTT                                                                                                            <  SRR19973061.692259/150‑1 (MQ=60)
                                       TGATAGATCAATTGAATTATCCGCCTAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAATACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGT                                                                                                          <  SRR19973061.2913588/150‑1 (MQ=60)
                                         ATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAA                                                                                                        >  SRR19973061.1106994/1‑150 (MQ=60)
                                          TAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAAC                                                                                                       >  SRR19973061.1164458/1‑150 (MQ=60)
                                          TAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAAC                                                                                                       >  SRR19973061.1164459/1‑150 (MQ=60)
                                          TAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAAC                                                                                                       <  SRR19973061.1223188/150‑1 (MQ=60)
                                           AGATCAATTGACTTATCCGCCGAAAATGCGCGCCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACT                                                                                                      <  SRR19973061.1141766/150‑1 (MQ=60)
                                               CAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCAC                                                                                                  >  SRR19973061.816527/1‑150 (MQ=60)
                                               CAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCAC                                                                                                  >  SRR19973061.1450978/1‑150 (MQ=60)
                                                 ATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCG                                                                                                >  SRR19973061.1715597/1‑150 (MQ=60)
                                                    GACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACT                                                                                             <  SRR19973061.2378467/150‑1 (MQ=60)
                                                       TTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAAATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCAT                                                                                          >  SRR19973061.3507876/1‑150 (MQ=60)
                                                        TATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATG                                                                                         >  SRR19973061.3710945/1‑150 (MQ=60)
                                                          TCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTC                                                                                       <  SRR19973061.993309/150‑1 (MQ=60)
                                                          TCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTC                                                                                       >  SRR19973061.1655473/1‑150 (MQ=60)
                                                               CGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCT                                                                                  >  SRR19973061.203215/1‑150 (MQ=60)
                                                                GAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTA                                                                                 <  SRR19973061.1549726/150‑1 (MQ=60)
                                                                 AAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTAC                                                                                <  SRR19973061.3425933/150‑1 (MQ=60)
                                                                   AATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTT                                                                              <  SRR19973061.1611174/150‑1 (MQ=60)
                                                                   AATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTT                                                                              >  SRR19973061.3269363/1‑150 (MQ=60)
                                                                   AATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTT                                                                              >  SRR19973061.3269229/1‑150 (MQ=60)
                                                                          GTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTC                                                                       <  SRR19973061.3390373/150‑1 (MQ=60)
                                                                            CGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTT                                                                     <  SRR19973061.1054597/150‑1 (MQ=60)
                                                                             GGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTC                                                                    <  SRR19973061.2591559/150‑1 (MQ=60)
                                                                             GGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTC                                                                    <  SRR19973061.1746834/150‑1 (MQ=60)
                                                                              GAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCA                                                                   >  SRR19973061.225289/1‑150 (MQ=60)
                                                                              GAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGAAGAGATGAAGACATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCAACTCATGTCGTGCTACTACATGTTCTTCA                                                                   >  SRR19973061.225428/1‑150 (MQ=60)
                                                                                AAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAG                                                                 <  SRR19973061.324993/150‑1 (MQ=60)
                                                                                 AGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGG                                                                >  SRR19973061.1524823/1‑150 (MQ=60)
                                                                                 AGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGG                                                                >  SRR19973061.3189782/1‑150 (MQ=60)
                                                                                  GCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGG                                                               <  SRR19973061.421639/150‑1 (MQ=60)
                                                                                   CGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGT                                                              <  SRR19973061.3253733/150‑1 (MQ=60)
                                                                                    GCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTA                                                             <  SRR19973061.2968249/150‑1 (MQ=60)
                                                                                    GCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTA                                                             >  SRR19973061.184432/1‑150 (MQ=60)
                                                                                     CCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAA                                                            <  SRR19973061.139645/150‑1 (MQ=60)
                                                                                     CCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAA                                                            <  SRR19973061.1158282/150‑1 (MQ=60)
                                                                                       AGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAAT                                                          <  SRR19973061.3609682/150‑1 (MQ=60)
                                                                                          CGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACC                                                       <  SRR19973061.3017511/150‑1 (MQ=60)
                                                                                            CTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGC                                                     >  SRR19973061.1043280/1‑150 (MQ=60)
                                                                                                      TGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGAC                                           >  SRR19973061.3446655/1‑150 (MQ=60)
                                                                                                      TGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGAC                                           >  SRR19973061.1467795/1‑150 (MQ=60)
                                                                                                        TAACAATAAAGACGTTTGCCCACAGGGCAGAGATGATGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGA                                         <  SRR19973061.60201/150‑1 (MQ=60)
                                                                                                        TAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGA                                         <  SRR19973061.3358437/150‑1 (MQ=60)
                                                                                                        TAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGA                                         <  SRR19973061.60135/150‑1 (MQ=60)
                                                                                                        TAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGA                                         <  SRR19973061.1454358/150‑1 (MQ=60)
                                                                                                        TAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGA                                         <  SRR19973061.1454432/150‑1 (MQ=60)
                                                                                                        TAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGA                                         <  SRR19973061.1976207/150‑1 (MQ=60)
                                                                                                         AACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAA                                        <  SRR19973061.2022506/150‑1 (MQ=60)
                                                                                                            AATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGA                                     <  SRR19973061.596546/150‑1 (MQ=60)
                                                                                                               AAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTCCTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGG                                  >  SRR19973061.2723897/1‑150 (MQ=60)
                                                                                                               AAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGG                                  <  SRR19973061.914314/150‑1 (MQ=60)
                                                                                                               AAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGG                                  <  SRR19973061.914290/150‑1 (MQ=60)
                                                                                                               AAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGG                                  >  SRR19973061.1361756/1‑150 (MQ=60)
                                                                                                               AAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGG                                  <  SRR19973061.2219060/150‑1 (MQ=60)
                                                                                                               AAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGG                                  <  SRR19973061.2727434/150‑1 (MQ=60)
                                                                                                                AAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGT                                 >  SRR19973061.3418970/1‑150 (MQ=60)
                                                                                                                 AGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTC                                <  SRR19973061.1112827/150‑1 (MQ=60)
                                                                                                                 AGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTC                                <  SRR19973061.578288/150‑1 (MQ=60)
                                                                                                                  GACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCG                               >  SRR19973061.2620610/1‑150 (MQ=60)
                                                                                                                    CGTTTGCCCACCGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGC                             <  SRR19973061.2158475/150‑1 (MQ=60)
                                                                                                                    CGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGC                             <  SRR19973061.1270327/150‑1 (MQ=60)
                                                                                                                      TTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCT                           <  SRR19973061.592573/150‑1 (MQ=60)
                                                                                                                      TTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCT                           <  SRR19973061.593006/150‑1 (MQ=60)
                                                                                                                      TTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCT                           <  SRR19973061.872288/150‑1 (MQ=60)
                                                                                                                       TTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTG                          <  SRR19973061.2691913/150‑1 (MQ=60)
                                                                                                                         GCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGAT                        <  SRR19973061.2993419/150‑1 (MQ=60)
                                                                                                                           CCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTC                      <  SRR19973061.3423275/150‑1 (MQ=60)
                                                                                                                            CACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCG                     >  SRR19973061.2634178/1‑150 (MQ=60)
                                                                                                                               AGGGCAGAGATGATGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCA                  <  SRR19973061.3677875/150‑1 (MQ=60)
                                                                                                                                TGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAG                 >  SRR19973061.2915132/1‑150 (MQ=60)
                                                                                                                                  GCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTT               <  SRR19973061.3261627/150‑1 (MQ=60)
                                                                                                                                    AGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGT             >  SRR19973061.1944247/1‑150 (MQ=60)
                                                                                                                                      AGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTC           >  SRR19973061.1420014/1‑150 (MQ=60)
                                                                                                                                       GATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCC          >  SRR19973061.724848/1‑150 (MQ=60)
                                                                                                                                       GATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCC          <  SRR19973061.15299/150‑1 (MQ=60)
                                                                                                                                        ATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCG         <  SRR19973061.1310350/150‑1 (MQ=60)
                                                                                                                                         TGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGT        >  SRR19973061.3384553/1‑150 (MQ=60)
                                                                                                                                         TGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGT        >  SRR19973061.1237008/1‑150 (MQ=60)
                                                                                                                                           AAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTG      >  SRR19973061.1308084/1‑150 (MQ=60)
                                                                                                                                             GTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCC    <  SRR19973061.866168/150‑1 (MQ=60)
                                                                                                                                               CATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCAC  >  SRR19973061.388472/1‑150 (MQ=60)
                                                                                                                                               CATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCAC  >  SRR19973061.37487/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                  
ATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCAC  >  CP009273/1963476‑1963768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑