Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,963,622 A→T 19.7% intergenic (‑1/+18) cheR ← / ← tap chemotaxis regulator, protein‑glutamate methyltransferase/methyl‑accepting protein IV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,963,6220AT19.7% 755.5 / 169.5 324intergenic (‑1/+18)cheR/tapchemotaxis regulator, protein‑glutamate methyltransferase/methyl‑accepting protein IV
Reads supporting (aligned to +/- strand):  ref base A (127/132);  new base T (27/37);  total (155/169)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

ACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGC  >  CP009273/1963473‑1963771
                                                                                                                                                     |                                                                                                                                                     
aCCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATa                                                                                                                                                       <  2:3272787/150‑1 (MQ=255)
  cATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTgc                                                                                                                                                     <  2:2278999/150‑1 (MQ=255)
   aTGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTACCAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAgcg                                                                                                                                                    <  2:1797115/150‑1 (MQ=255)
     gTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGcc                                                                                                                                                  <  1:1640137/150‑1 (MQ=255)
         cgTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTtct                                                                                                                                              >  2:1327388/1‑150 (MQ=255)
          gTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTtctc                                                                                                                                             <  2:3640442/150‑1 (MQ=255)
          gTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTtctc                                                                                                                                             <  2:3752181/150‑1 (MQ=255)
           tttATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCa                                                                                                                                            >  1:938710/1‑150 (MQ=255)
               tGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCa                                                                                                                                        <  2:5229414/150‑1 (MQ=255)
                   cAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTc                                                                                                                                    <  1:4055082/150‑1 (MQ=255)
                    aGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCa                                                                                                                                   <  1:4724880/150‑1 (MQ=255)
                    aGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCa                                                                                                                                   >  2:687063/1‑150 (MQ=255)
                     gCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAg                                                                                                                                  <  2:4864084/150‑1 (MQ=255)
                        aGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGAt                                                                                                                               >  2:4155484/1‑150 (MQ=255)
                            cGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCa                                                                                                                           <  2:2191271/150‑1 (MQ=255)
                             gATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCAc                                                                                                                          >  1:4431751/1‑150 (MQ=255)
                             gATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCAc                                                                                                                          >  2:3166934/1‑150 (MQ=255)
                             gATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCAc                                                                                                                          >  2:285839/1‑150 (MQ=255)
                              aTCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACt                                                                                                                         >  1:2428232/1‑150 (MQ=255)
                                    gCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCa                                                                                                                   >  1:56444/1‑150 (MQ=255)
                                       cGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAAtt                                                                                                                >  1:543477/1‑150 (MQ=255)
                                       cGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAAtt                                                                                                                >  1:1672910/1‑150 (MQ=255)
                                         ttGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTg                                                                                                              <  1:4010267/150‑1 (MQ=255)
                                          tGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGt                                                                                                             <  1:3891751/150‑1 (MQ=255)
                                           gATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTa                                                                                                            >  1:5109412/1‑150 (MQ=255)
                                            aTAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTaa                                                                                                           >  1:1859787/1‑150 (MQ=255)
                                            aTAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCACTTTGTaa                                                                                                           >  2:4541004/1‑150 (MQ=255)
                                            aTAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTaa                                                                                                           >  2:459167/1‑150 (MQ=255)
                                            aTAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTaa                                                                                                           >  2:469198/1‑150 (MQ=255)
                                             tAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAAc                                                                                                          <  1:3786095/150‑1 (MQ=255)
                                               gATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTg                                                                                                        <  2:2729934/150‑1 (MQ=255)
                                                  cAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCAc                                                                                                     >  1:4160368/1‑150 (MQ=255)
                                                   aaTTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCAcc                                                                                                    <  2:5045612/150‑1 (MQ=255)
                                                    aTTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCg                                                                                                   >  2:1643714/1‑150 (MQ=255)
                                                       gACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACt                                                                                                >  2:4518235/1‑150 (MQ=255)
                                                          ttATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCAt                                                                                             <  2:1439359/150‑1 (MQ=255)
                                                          ttATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCAt                                                                                             <  2:2941951/150‑1 (MQ=255)
                                                           tATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATg                                                                                            >  1:2316262/1‑150 (MQ=255)
                                                            aTCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGt                                                                                           >  2:2211695/1‑150 (MQ=255)
                                                             tCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTc                                                                                          >  1:1161873/1‑150 (MQ=255)
                                                             tCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTc                                                                                          <  1:2216582/150‑1 (MQ=255)
                                                              ccgccgAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCg                                                                                         <  1:3474500/150‑1 (MQ=255)
                                                               cgccgAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGt                                                                                        <  2:2508172/150‑1 (MQ=255)
                                                                  cgAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATCGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCt                                                                                     >  2:1983168/1‑150 (MQ=255)
                                                                   gAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTa                                                                                    <  1:1197497/150‑1 (MQ=255)
                                                                         gcgcGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCAt                                                                              <  1:2551467/150‑1 (MQ=255)
                                                                               cGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttctt                                                                        <  2:56232/150‑1 (MQ=255)
                                                                               cGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttctt                                                                        >  1:3041778/1‑150 (MQ=255)
                                                                               cGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttctt                                                                        <  2:3798606/150‑1 (MQ=255)
                                                                                ggAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttcttc                                                                       <  2:802152/150‑1 (MQ=255)
                                                                                ggAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttcttc                                                                       <  2:4976136/150‑1 (MQ=255)
                                                                                       gcCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTa                                                                >  1:4134778/1‑150 (MQ=255)
                                                                                       gcCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTa                                                                >  1:130560/1‑150 (MQ=255)
                                                                                        ccAGGCGCTCTGTCATCTGTAACAATAAAGACGTTTTCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCAATGGCGCAATTTGTAACTGCACCGACTCATGTAGTGCTACTTCATGTTCTTCAAGGGTaa                                                               >  2:341892/1‑150 (MQ=255)
                                                                                             cgcTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATAcc                                                          <  1:3396155/150‑1 (MQ=255)
                                                                                                tCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGcc                                                       <  1:1756667/150‑1 (MQ=255)
                                                                                                tCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGcc                                                       <  1:1531272/150‑1 (MQ=255)
                                                                                                  ggTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCcac                                                     >  1:2652177/1‑150 (MQ=255)
                                                                                                  ggTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCcac                                                     <  2:60262/150‑1 (MQ=255)
                                                                                                   gTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCcaca                                                    <  1:4828410/150‑1 (MQ=255)
                                                                                                    tCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCacac                                                   >  2:90173/1‑150 (MQ=255)
                                                                                                      aTCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACAcgc                                                 >  2:1264591/1‑150 (MQ=255)
                                                                                                      aTCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACAcgc                                                 <  1:5216151/150‑1 (MQ=255)
                                                                                                        cTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcga                                               >  2:3005621/1‑150 (MQ=255)
                                                                                                         tGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcgac                                              >  2:966295/1‑150 (MQ=255)
                                                                                                           tAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcgacga                                            >  2:2294113/1‑150 (MQ=255)
                                                                                                             aCAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGaaa                                          >  1:3264118/1‑150 (MQ=255)
                                                                                                              cAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAg                                         <  1:1008453/150‑1 (MQ=255)
                                                                                                                aTAAAGTCGTTTGCCCACCGGGCAGAGCTGTAGTCATAGCGCCTTCACAATCACTTCAGGTTACCACTGGCGCAATTTGTAACTGCACCGACACATGTCGTGCTACTTCATGTTCATCAACGGTAAATACCGCCACACGCGACGAAAGAt                                       <  2:4449474/150‑1 (MQ=255)
                                                                                                                aTAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGAt                                       <  1:428148/150‑1 (MQ=255)
                                                                                                                aTAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGAt                                       >  2:838733/1‑150 (MQ=255)
                                                                                                                 tAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATg                                      <  2:13819/150‑1 (MQ=255)
                                                                                                                 tAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCACCACACGCGACGAAAGATg                                      <  2:2096286/150‑1 (MQ=255)
                                                                                                                   aaGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGt                                    <  1:4861808/150‑1 (MQ=255)
                                                                                                                    aGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTTATGTTCTTCAAGGGTAAATACCGCCACACGCGACTAAAGATGGTc                                   <  2:1685113/150‑1 (MQ=255)
                                                                                                                      aCGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCgg                                 <  2:2668377/150‑1 (MQ=255)
                                                                                                                        gTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGcc                               >  1:2782195/1‑150 (MQ=255)
                                                                                                                        gTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGcc                               >  1:730596/1‑150 (MQ=255)
                                                                                                                         tttGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCt                              <  2:3468476/150‑1 (MQ=255)
                                                                                                                         tttGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCt                              <  1:3081603/150‑1 (MQ=255)
                                                                                                                             cccACAGGGCAGAGATGAAGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGAtt                          <  2:2395507/150‑1 (MQ=255)
                                                                                                                               cacaGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCg                        >  2:1876519/1‑150 (MQ=255)
                                                                                                                                   gggCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAg                    <  1:3655124/150‑1 (MQ=255)
                                                                                                                                    ggCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgt                   <  2:938710/150‑1 (MQ=255)
                                                                                                                                     gCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgtt                  <  1:2105234/150‑1 (MQ=255)
                                                                                                                                      cAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttg                 <  1:859575/150‑1 (MQ=255)
                                                                                                                                      cAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttg                 <  2:4363191/150‑1 (MQ=255)
                                                                                                                                       agagATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttgt                >  1:4485456/1‑150 (MQ=255)
                                                                                                                                       agagATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttgt                >  2:1119401/1‑150 (MQ=255)
                                                                                                                                       agagATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttgt                >  2:2978135/1‑150 (MQ=255)
                                                                                                                                        gagaTGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttgtt               >  2:361044/1‑150 (MQ=255)
                                                                                                                                         agaTGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTc              <  2:2111122/150‑1 (MQ=255)
                                                                                                                                           aTGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCg            >  1:984254/1‑150 (MQ=255)
                                                                                                                                             gAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGtt          <  2:2750306/150‑1 (MQ=255)
                                                                                                                                             gAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGtt          >  2:5258462/1‑150 (MQ=255)
                                                                                                                                              aaGTCATTGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTg         <  1:4636237/150‑1 (MQ=255)
                                                                                                                                              aaGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTg         >  1:3621598/1‑150 (MQ=255)
                                                                                                                                                 tCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCa      >  2:3024853/1‑150 (MQ=255)
                                                                                                                                                  cATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCAc     <  2:2600825/150‑1 (MQ=255)
                                                                                                                                                    tAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCAccg   >  2:3973632/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGC  >  CP009273/1963473‑1963771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A