Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,574,820 C→A A609E (GCG→GAG)  tktB → transketolase 2, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,574,8200CA100.0% 747.8 / NA 222A609E (GCG→GAG) tktBtransketolase 2, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (114/108);  total (114/108)

Only 100 of 222 total aligned reads displayed.

TGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAG  >  CP009273/2574673‑2574966
                                                                                                                                                   |                                                                                                                                                  
 ggAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGt                                                                                                                                                 >  1:2582512/1‑150 (MQ=255)
 ggAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGt                                                                                                                                                 <  1:477435/150‑1 (MQ=255)
   aaaTTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTgg                                                                                                                                               >  2:1371087/1‑150 (MQ=255)
     aTTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGaa                                                                                                                                             <  1:213635/150‑1 (MQ=255)
         cccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAg                                                                                                                                         <  2:2214987/150‑1 (MQ=255)
            tGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTa                                                                                                                                      <  2:1322642/150‑1 (MQ=255)
            tGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTa                                                                                                                                      <  2:2138193/150‑1 (MQ=255)
              cAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTAtt                                                                                                                                    >  2:3293830/1‑150 (MQ=255)
              cAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTCGCGGCTCGCGTGGAGGTGGAAGCAGGTAtt                                                                                                                                    <  1:390935/150‑1 (MQ=255)
               aaGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTg                                                                                                                                   <  2:1677801/150‑1 (MQ=255)
               aaGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTg                                                                                                                                   >  1:2478793/1‑150 (MQ=255)
                aGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAg                                                                                                                                            >  2:3783104/1‑140 (MQ=255)
                aGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAg                                                                                                                                            >  2:2503444/1‑140 (MQ=255)
                aGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAg                                                                                                                                            <  1:2503444/140‑1 (MQ=255)
                 gCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGcc                                                                                                                                 <  2:3674739/150‑1 (MQ=255)
                       gagaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTAc                                                                                                                           >  1:1645214/1‑150 (MQ=255)
                       gagaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTAc                                                                                                                           <  1:1401035/150‑1 (MQ=255)
                        agaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACt                                                                                                                          >  2:2875883/1‑150 (MQ=255)
                           aaTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGt                                                                                                                       <  1:1260827/150‑1 (MQ=255)
                            aTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTa                                                                                                                      >  2:874880/1‑150 (MQ=255)
                            aTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTa                                                                                                                      >  2:2718060/1‑150 (MQ=255)
                             ttAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTAc                                                                                                                     <  1:3052136/150‑1 (MQ=255)
                             ttAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTAc                                                                                                                     >  2:227472/1‑150 (MQ=255)
                                gCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAg                                                                                                                  <  1:2175693/150‑1 (MQ=255)
                                   ggAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTAt                                                                                                               >  1:2128705/1‑150 (MQ=255)
                                    gagaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATg                                                                                                              >  1:1471677/1‑150 (MQ=255)
                                      gaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGtt                                                                                                            <  2:1768437/150‑1 (MQ=255)
                                        aGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTgg                                                                                                          >  1:3483826/1‑150 (MQ=255)
                                         ggTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGt                                                                                                         >  1:3778136/1‑150 (MQ=255)
                                         ggTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGt                                                                                                         >  1:2894844/1‑150 (MQ=255)
                                         ggTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGt                                                                                                         >  1:1884164/1‑150 (MQ=255)
                                          gTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTc                                                                                                        <  2:2812447/150‑1 (MQ=255)
                                          gTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTc                                                                                                        >  2:1661407/1‑150 (MQ=255)
                                          gTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTc                                                                                                        >  1:1829500/1‑150 (MQ=255)
                                            cGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTg                                                                                                      >  2:3723789/1‑150 (MQ=255)
                                            cGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTg                                                                                                      >  2:3187590/1‑150 (MQ=255)
                                                 tGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAgg                                                                                                 >  2:849100/1‑150 (MQ=255)
                                                  gTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAggg                                                                                                <  2:1198876/150‑1 (MQ=255)
                                                   tACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAgggg                                                                                               >  1:1343908/1‑150 (MQ=255)
                                                     cgcgTAGATTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGACGACTATCGGGAGACGGTGTTGCCTTCAAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTAGTGCCGATTACTGGTACACGTCAGTGGGTCTGAAAGGGGCa                                                                                             >  2:2613894/1‑150 (MQ=255)
                                                       cgTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAAt                                                                                           >  2:3474246/1‑150 (MQ=255)
                                                        gTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAAtt                                                                                          >  2:2689769/1‑150 (MQ=255)
                                                          aGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGt                                                                                        <  2:2599469/150‑1 (MQ=255)
                                                           gTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTc                                                                                       >  2:788540/1‑150 (MQ=255)
                                                              tCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCggg                                                                                    >  1:1747072/1‑150 (MQ=255)
                                                                ccTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGAt                                                                                  >  1:1154462/1‑150 (MQ=255)
                                                                   gCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGAc                                                                               <  1:3409745/150‑1 (MQ=255)
                                                                   gCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGAc                                                                               >  1:3142328/1‑150 (MQ=255)
                                                                     ccTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACgg                                                                             >  1:1049009/1‑150 (MQ=255)
                                                                     ccTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACgg                                                                             >  1:2135003/1‑150 (MQ=255)
                                                                      cTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACggg                                                                            >  2:950723/1‑150 (MQ=255)
                                                                        cGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGtt                                                                          >  1:803159/1‑150 (MQ=255)
                                                                          aCCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTAc                                                                        <  2:3233005/150‑1 (MQ=255)
                                                                            cGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACgg                                                                      >  2:695134/1‑150 (MQ=255)
                                                                             gATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACggg                                                                     >  2:75505/1‑150 (MQ=255)
                                                                              atatTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACgggg                                                                    >  1:766856/1‑150 (MQ=255)
                                                                              atatTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACgggg                                                                    >  1:3639539/1‑150 (MQ=255)
                                                                              atatTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACgggg                                                                    <  2:2763797/150‑1 (MQ=255)
                                                                               tatTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGa                                                                   <  2:1352271/150‑1 (MQ=255)
                                                                                 ttttCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAt                                                                 <  1:2412462/150‑1 (MQ=255)
                                                                                 ttttCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAt                                                                 <  1:2412479/150‑1 (MQ=255)
                                                                                        cGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTcc                                                          <  1:3019120/150‑1 (MQ=255)
                                                                                           ccAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggc                                                       >  1:59001/1‑150 (MQ=255)
                                                                                            cAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggcg                                                      <  2:1796754/150‑1 (MQ=255)
                                                                                             aGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggcgg                                                     <  1:849306/150‑1 (MQ=255)
                                                                                             aGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggcgg                                                     >  2:714734/1‑150 (MQ=255)
                                                                                              ggATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGa                                                    <  2:2384914/150‑1 (MQ=255)
                                                                                               gATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGAt                                                   >  1:916638/1‑150 (MQ=255)
                                                                                               gATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGAt                                                   <  2:1728540/150‑1 (MQ=255)
                                                                                                aTGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATa                                                  <  2:864718/150‑1 (MQ=255)
                                                                                                aTGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATa                                                  <  2:2302457/150‑1 (MQ=255)
                                                                                                  gAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAg                                                >  2:1977860/1‑150 (MQ=255)
                                                                                                    ggACTATCGGGAGTCGGTGTTGCCTTCTAACGGTGCGACTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCt                                              <  1:2620825/150‑1 (MQ=255)
                                                                                                       atatCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGtt                                           <  1:2429727/150‑1 (MQ=255)
                                                                                                            gggAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGt                                      >  1:1955619/1‑150 (MQ=255)
                                                                                                            gggAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGt                                      >  2:3589505/1‑150 (MQ=255)
                                                                                                             ggAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGtt                                     >  2:2549207/1‑150 (MQ=255)
                                                                                                             ggAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGtt                                     >  2:1578315/1‑150 (MQ=255)
                                                                                                              gAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTc                                    <  2:3347630/150‑1 (MQ=255)
                                                                                                                   ggTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTgg                               <  1:2357477/150‑1 (MQ=255)
                                                                                                                    gtgtTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGc                              >  2:3498200/1‑150 (MQ=255)
                                                                                                                     tgtTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCt                             >  1:1694918/1‑150 (MQ=255)
                                                                                                                      gtTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCtt                            <  2:3084428/150‑1 (MQ=255)
                                                                                                                      gtTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCtt                            >  1:1613124/1‑150 (MQ=255)
                                                                                                                             tCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTAccgcc                     <  2:2582512/150‑1 (MQ=255)
                                                                                                                             tCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTGGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTAccgcc                     <  2:3516317/150‑1 (MQ=255)
                                                                                                                              cTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTAccgccg                    >  2:1297600/1‑150 (MQ=255)
                                                                                                                                 aCGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCgaga                 <  1:1469297/150‑1 (MQ=255)
                                                                                                                                   gTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAat               >  2:2896094/1‑150 (MQ=255)
                                                                                                                                     tGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAatat             <  1:3425466/150‑1 (MQ=255)
                                                                                                                                     tGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAatat             <  2:1471677/150‑1 (MQ=255)
                                                                                                                                     tGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAatat             <  1:3529988/150‑1 (MQ=255)
                                                                                                                                        ggCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATtgt          >  2:1934251/1‑150 (MQ=255)
                                                                                                                                         gCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATtgtg         <  2:1154462/150‑1 (MQ=255)
                                                                                                                                          cTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTgg        >  1:2048473/1‑150 (MQ=255)
                                                                                                                                              cgTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCaaa    >  1:230078/1‑150 (MQ=255)
                                                                                                                                              cgTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCaaa    >  2:123912/1‑150 (MQ=255)
                                                                                                                                               gTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCaaaa   >  1:3785564/1‑150 (MQ=255)
                                                                                                                                                tGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAg  >  2:600527/1‑150 (MQ=255)
                                                                                                                                                tGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAg  <  2:2370559/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAG  >  CP009273/2574673‑2574966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCG  >  CP009273/2574671‑2574968
                                                                                                                                                     |                                                                                                                                                    
GATGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGA                                                                                                                                                      >  SRR19973061.3092378/1‑150 (MQ=60)
 ATGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAG                                                                                                                                                     >  SRR19973061.796378/1‑150 (MQ=60)
 ATGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAG                                                                                                                                                     <  SRR19973061.2362807/150‑1 (MQ=60)
 ATGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTGTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAG                                                                                                                                                     <  SRR19973061.1622325/150‑1 (MQ=60)
  TGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGG                                                                                                                                                    >  SRR19973061.1066725/1‑150 (MQ=60)
   GGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGT                                                                                                                                                   >  SRR19973061.2589125/1‑150 (MQ=60)
   GGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGT                                                                                                                                                   <  SRR19973061.478719/150‑1 (MQ=60)
   GGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGT                                                                                                                                                   <  SRR19973061.1207627/150‑1 (MQ=60)
       ATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAA                                                                                                                                               <  SRR19973061.214267/150‑1 (MQ=60)
        cttccgatctAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAG                                                                                                                                              <  SRR19973061.3792750/140‑1 (MQ=60)
        cttccgatctAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAG                                                                                                                                              <  SRR19973061.2509819/140‑1 (MQ=60)
              TGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTA                                                                                                                                        >  SRR19973061.3798860/1‑150 (MQ=60)
                CAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTCGCGGCTCGCGTGGAGGTGGAAGCAGGTATT                                                                                                                                      <  SRR19973061.392004/150‑1 (MQ=60)
                 AAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTG                                                                                                                                     >  SRR19973061.2485102/1‑150 (MQ=60)
                    CGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCG                                                                                                                                  <  SRR19973061.204633/150‑1 (MQ=60)
                         GAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTAC                                                                                                                             >  SRR19973061.1649346/1‑150 (MQ=60)
                         GAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTAC                                                                                                                             <  SRR19973061.1404574/150‑1 (MQ=60)
                             AATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGT                                                                                                                         <  SRR19973061.1263942/150‑1 (MQ=60)
                               TTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTAC                                                                                                                       <  SRR19973061.3060020/150‑1 (MQ=60)
                                TAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACA                                                                                                                      >  SRR19973061.1732883/1‑150 (MQ=60)
                                  GCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAG                                                                                                                    <  SRR19973061.2181163/150‑1 (MQ=60)
                                     GGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTCCAAGTAT                                                                                                                 >  SRR19973061.1786711/1‑150 (MQ=60)
                                     GGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTAT                                                                                                                 >  SRR19973061.2134818/1‑150 (MQ=60)
                                     GGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTAT                                                                                                                 >  SRR19973061.2134051/1‑150 (MQ=60)
                                      GAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATG                                                                                                                >  SRR19973061.1475374/1‑150 (MQ=60)
                                       AGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGT                                                                                                               >  SRR19973061.925505/1‑150 (MQ=60)
                                       AGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGT                                                                                                               >  SRR19973061.2595072/1‑150 (MQ=60)
                                        GAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTT                                                                                                              <  SRR19973061.3750893/150‑1 (MQ=60)
                                         AAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTG                                                                                                             <  SRR19973061.2406856/150‑1 (MQ=60)
                                          AGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGG                                                                                                            >  SRR19973061.3492735/1‑150 (MQ=60)
                                           GGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGT                                                                                                           >  SRR19973061.2902269/1‑150 (MQ=60)
                                           GGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGT                                                                                                           >  SRR19973061.3787779/1‑150 (MQ=60)
                                           GGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGT                                                                                                           <  SRR19973061.393648/150‑1 (MQ=60)
                                           GGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGT                                                                                                           >  SRR19973061.1888846/1‑150 (MQ=60)
                                            GTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTC                                                                                                          >  SRR19973061.1834086/1‑150 (MQ=60)
                                               GCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGA                                                                                                       <  SRR19973061.1641997/150‑1 (MQ=60)
                                                     TACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGG                                                                                                 >  SRR19973061.1347286/1‑150 (MQ=60)
                                                       CGCGTAGTGTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCA                                                                                               <  SRR19973061.3195771/150‑1 (MQ=60)
                                                        GCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAA                                                                                              <  SRR19973061.2335475/150‑1 (MQ=60)
                                                             GTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTC                                                                                         <  SRR19973061.350409/150‑1 (MQ=60)
                                                              TTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCG                                                                                        <  SRR19973061.569785/150‑1 (MQ=60)
                                                              TTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCG                                                                                        >  SRR19973061.312005/1‑150 (MQ=60)
                                                                TCCCTTCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGG                                                                                      <  SRR19973061.189896/150‑1 (MQ=60)
                                                                TCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGG                                                                                      >  SRR19973061.1751462/1‑150 (MQ=60)
                                                                TCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGG                                                                                      >  SRR19973061.1751340/1‑150 (MQ=60)
                                                                TCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGG                                                                                      <  SRR19973061.877150/150‑1 (MQ=60)
                                                                  CCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGAT                                                                                    >  SRR19973061.1157393/1‑150 (MQ=60)
                                                                  CCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGAT                                                                                    >  SRR19973061.3038/1‑150 (MQ=60)
                                                                     GCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGAC                                                                                 <  SRR19973061.3418501/150‑1 (MQ=60)
                                                                     GCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGAC                                                                                 >  SRR19973061.3150416/1‑150 (MQ=60)
                                                                       CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGG                                                                               >  SRR19973061.3003386/1‑150 (MQ=60)
                                                                       CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGG                                                                               >  SRR19973061.1051698/1‑150 (MQ=60)
                                                                       CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGG                                                                               >  SRR19973061.2140468/1‑150 (MQ=60)
                                                                       CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGG                                                                               >  SRR19973061.2140365/1‑150 (MQ=60)
                                                                          CGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTT                                                                            >  SRR19973061.805266/1‑150 (MQ=60)
                                                                            ACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTAC                                                                          <  SRR19973061.2420034/150‑1 (MQ=60)
                                                                             CCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACG                                                                         >  SRR19973061.2235646/1‑150 (MQ=60)
                                                                                ATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGG                                                                      >  SRR19973061.3648840/1‑150 (MQ=60)
                                                                                ATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGG                                                                      >  SRR19973061.768867/1‑150 (MQ=60)
                                                                                  ATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAA                                                                    >  SRR19973061.345822/1‑150 (MQ=60)
                                                                                   TTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAT                                                                   <  SRR19973061.2418637/150‑1 (MQ=60)
                                                                                   TTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAT                                                                   <  SRR19973061.2418631/150‑1 (MQ=60)
                                                                                   TTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAT                                                                   <  SRR19973061.2418620/150‑1 (MQ=60)
                                                                                   TTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTAGTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAT                                                                   <  SRR19973061.2418783/150‑1 (MQ=60)
                                                                                    TTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATC                                                                  >  SRR19973061.3605387/1‑150 (MQ=60)
                                                                                    TTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATC                                                                  >  SRR19973061.1968070/1‑150 (MQ=60)
                                                                                      TCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTG                                                                >  SRR19973061.2455086/1‑150 (MQ=60)
                                                                                          CGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCC                                                            <  SRR19973061.3026914/150‑1 (MQ=60)
                                                                                             CCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGC                                                         <  SRR19973061.228139/150‑1 (MQ=60)
                                                                                             CCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGC                                                         >  SRR19973061.59230/1‑150 (MQ=60)
                                                                                               AGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGG                                                       <  SRR19973061.851316/150‑1 (MQ=60)
                                                                                               AGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGG                                                       <  SRR19973061.851523/150‑1 (MQ=60)
                                                                                                GGATGAGGAATATCGGGAGTCGGTGTTGNCTTCTAACGTTGCGGCTCGCGTGGANGTGGANGCAGGTATTNCCNATNNCTGGTNCAAGTATGNTGNTNTGAAAGGGGNANTTGTCGGGANTACGGGTTACGGNNAATCTGCTCCGGCNGA                                                      >  SRR19973061.1306537/1‑150 (MQ=60)
                                                                                                 GATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGAT                                                     >  SRR19973061.918985/1‑150 (MQ=60)
                                                                                                   TGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAA                                                   >  SRR19973061.2116545/1‑150 (MQ=60)
                                                                                                   TGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAA                                                   <  SRR19973061.3774867/150‑1 (MQ=60)
                                                                                                    GAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAG                                                  <  SRR19973061.2799151/150‑1 (MQ=60)
                                                                                                     AGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGC                                                 <  SRR19973061.1470472/150‑1 (MQ=60)
                                                                                                      GGACTATCGGGAGTCGGTGTTGCCTTCTAACGGTGCGACTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCT                                                <  SRR19973061.2627552/150‑1 (MQ=60)
                                                                                                       GAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGTTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTG                                               <  SRR19973061.2495895/150‑1 (MQ=60)
                                                                                                         ATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTT                                             <  SRR19973061.526082/150‑1 (MQ=60)
                                                                                                         ATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTT                                             <  SRR19973061.2435931/150‑1 (MQ=60)
                                                                                                              GGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGT                                        >  SRR19973061.1960502/1‑150 (MQ=60)
                                                                                                                GAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTC                                      <  SRR19973061.1697527/150‑1 (MQ=60)
                                                                                                                   TCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTT                                   <  SRR19973061.214812/150‑1 (MQ=60)
                                                                                                                   TCGGTGGTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTT                                   <  SRR19973061.2837051/150‑1 (MQ=60)
                                                                                                                     GGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGG                                 <  SRR19973061.2363468/150‑1 (MQ=60)
                                                                                                                      GTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGC                                >  SRR19973061.1604491/1‑150 (MQ=60)
                                                                                                                      GTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGC                                >  SRR19973061.332460/1‑150 (MQ=60)
                                                                                                                       TGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCT                               >  SRR19973061.1699174/1‑150 (MQ=60)
                                                                                                                        GTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTT                              >  SRR19973061.1617140/1‑150 (MQ=60)
                                                                                                                        GTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTT                              <  SRR19973061.790613/150‑1 (MQ=60)
                                                                                                                               TCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCC                       <  SRR19973061.1349051/150‑1 (MQ=60)
                                                                                                                                CTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCG                      <  SRR19973061.1306349/150‑1 (MQ=60)
                                                                                                                                  AACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAG                    <  SRR19973061.2537936/150‑1 (MQ=60)
                                                                                                                                   ACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGA                   <  SRR19973061.1474828/150‑1 (MQ=60)
                                                                                                                                   ACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGA                   <  SRR19973061.1472990/150‑1 (MQ=60)
                                                                                                                                       TGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATAT               <  SRR19973061.3539028/150‑1 (MQ=60)
                                                                                                                                       TGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATAT               <  SRR19973061.144878/150‑1 (MQ=60)
                                                                                                                                       TGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATAT               <  SRR19973061.3434253/150‑1 (MQ=60)
                                                                                                                                          GGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGT            >  SRR19973061.3424858/1‑150 (MQ=60)
                                                                                                                                            CTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGG          >  SRR19973061.2053622/1‑150 (MQ=60)
                                                                                                                                              CGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCA        <  SRR19973061.629930/150‑1 (MQ=60)
                                                                                                                                                CGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAA      >  SRR19973061.230750/1‑150 (MQ=60)
                                                                                                                                                 GTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAA     >  SRR19973061.3795215/1‑150 (MQ=60)
                                                                                                                                                 GTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAA     >  SRR19973061.1835905/1‑150 (MQ=60)
                                                                                                                                                    GAGGTGGATGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCG  <  SRR19973061.716596/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GATGGAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCG  >  CP009273/2574671‑2574968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑