Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,574,820 C→A 100% A609E (GCG→GAG)  tktB → transketolase 2, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,574,8200CA97.9% 593.4 / 3.2 192A609E (GCG→GAG) tktBtransketolase 2, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (94/94);  total (95/97)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

TTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCA  >  CP009273/2574679‑2574962
                                                                                                                                             |                                                                                                                                              
ttACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGa                                                                                                                                          <  2:1924725/148‑1 (MQ=255)
   cccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGc                                                                                                                                       <  2:534491/148‑1 (MQ=255)
   cccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGc                                                                                                                                       <  2:1097532/148‑1 (MQ=255)
   cccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGc                                                                                                                                       <  2:2299826/148‑1 (MQ=255)
   cccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTAGCGTGGAGGTGGAAGc                                                                                                                                       <  1:225509/148‑1 (MQ=255)
    ccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCa                                                                                                                                      <  1:39850/148‑1 (MQ=255)
    ccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCa                                                                                                                                      >  1:14393/1‑148 (MQ=255)
    ccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCa                                                                                                                                      <  2:3278081/148‑1 (MQ=255)
    ccTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCa                                                                                                                                      <  2:3276807/148‑1 (MQ=255)
     cTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGGGGAGGTGGAAGCAg                                                                                                                                     >  1:1537257/1‑148 (MQ=255)
              gCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCg                                                                                                                            <  1:3031566/148‑1 (MQ=255)
               cAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGACTATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGa                                                                                                                           >  2:2786580/1‑148 (MQ=255)
               cAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGa                                                                                                                           >  2:2114813/1‑148 (MQ=255)
               cAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGa                                                                                                                           >  1:1826047/1‑148 (MQ=255)
               cAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGa                                                                                                                           <  1:1414282/148‑1 (MQ=255)
                 gagaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGAtt                                                                                                                         <  2:1184882/148‑1 (MQ=255)
                  agaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTa                                                                                                                        >  2:1053488/1‑148 (MQ=255)
                   gaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTAc                                                                                                                       <  1:2430547/148‑1 (MQ=255)
                    aaaaaaGCAGGAGAACGTCGCAATGTACGCGTAGTTTCCCTTCCCTAGACCGATATTTTCGACGCCCAGGAAGAGGAATATCGGTAGTCGGTGTTGCCTTCTAACGTTTCGGATCGCGTGGAGGTGGAAGCAGGTATTGCAGATTaat                                                                                                                      >  2:809553/6‑146 (MQ=255)
                    aaaTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACt                                                                                                                      <  1:2721469/148‑1 (MQ=255)
                    aaaTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTACCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGGGGAGGTGGAAGCAGGTATTGCCGATTACt                                                                                                                      >  2:3417761/1‑148 (MQ=255)
                     aaTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTg                                                                                                                     <  2:511862/148‑1 (MQ=255)
                     aaTGAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTg                                                                                                                     <  2:1722341/148‑1 (MQ=255)
                      aTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTgg                                                                                                                    >  1:2512444/1‑148 (MQ=255)
                       ttAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGt                                                                                                                   <  1:673478/148‑1 (MQ=255)
                       ttAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGt                                                                                                                   >  1:1839916/1‑148 (MQ=255)
                          gCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACa                                                                                                                <  1:924211/148‑1 (MQ=255)
                             ggAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGt                                                                                                             >  2:1205898/1‑148 (MQ=255)
                              gagaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTa                                                                                                            >  1:2109440/1‑148 (MQ=255)
                               agaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTAt                                                                                                           >  1:957505/1‑148 (MQ=255)
                                  aGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGtt                                                                                                        <  1:1794479/148‑1 (MQ=255)
                                     tCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGt                                                                                                     >  2:426322/1‑148 (MQ=255)
                                     tCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGt                                                                                                     >  2:926790/1‑148 (MQ=255)
                                        cAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTg                                                                                                  >  1:3375790/1‑148 (MQ=255)
                                           tGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGaaa                                                                                               <  1:111555/148‑1 (MQ=255)
                                           tGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGaaa                                                                                               >  1:2993899/1‑148 (MQ=255)
                                              aCGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAggg                                                                                            >  1:2136853/1‑148 (MQ=255)
                                               cgcgTAGTGTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAgggg                                                                                           <  2:2469565/148‑1 (MQ=255)
                                                 cgTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCa                                                                                         <  1:3116773/148‑1 (MQ=255)
                                                 cgTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCa                                                                                         <  1:2545202/148‑1 (MQ=255)
                                                 cgTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCa                                                                                         >  1:1766748/1‑148 (MQ=255)
                                                 cgTAGTGTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCa                                                                                         <  2:1598945/148‑1 (MQ=255)
                                                     gTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTg                                                                                     <  1:3466453/148‑1 (MQ=255)
                                                     gTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTg                                                                                     <  1:1254771/148‑1 (MQ=255)
                                                       ttCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTc                                                                                   <  2:1782098/148‑1 (MQ=255)
                                                           cTGCCCTCGACCGATATTTTCGACGCCCAGGATGATGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGa                                                                               <  2:1661901/148‑1 (MQ=255)
                                                           cTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGa                                                                               >  1:1775824/1‑148 (MQ=255)
                                                              cccTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGa                                                                            >  2:1452044/1‑148 (MQ=255)
                                                                cTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACg                                                                          >  1:538925/1‑148 (MQ=255)
                                                                cTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACg                                                                          >  1:2852646/1‑148 (MQ=255)
                                                                 tCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACgg                                                                         <  1:1951890/148‑1 (MQ=255)
                                                                  cGACCGATATTTTCGACGCCCAGGATGAGGACTATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATTTTGGTCTGAAAGGGGCAATTGTCGGGATGACggg                                                                        >  1:3115470/1‑148 (MQ=255)
                                                                  cGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACggg                                                                        >  1:1272109/1‑148 (MQ=255)
                                                                  cGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACggg                                                                        >  2:1741981/1‑148 (MQ=255)
                                                                  cGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACggg                                                                        <  1:3484542/148‑1 (MQ=255)
                                                                        atatTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACgg                                                                  <  2:3488740/148‑1 (MQ=255)
                                                                        atatTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACgg                                                                  <  2:1752314/148‑1 (MQ=255)
                                                                        atatTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACgg                                                                  >  2:2838719/1‑148 (MQ=255)
                                                                          atTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACgggg                                                                >  2:1972478/1‑148 (MQ=255)
                                                                             ttCGTCGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAt                                                             <  1:3420138/148‑1 (MQ=255)
                                                                             ttCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAt                                                             <  2:740580/148‑1 (MQ=255)
                                                                             ttCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAt                                                             <  2:2601623/148‑1 (MQ=255)
                                                                             ttCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAAt                                                             >  2:3332477/1‑148 (MQ=255)
                                                                                 aCGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGc                                                         >  1:2514664/1‑148 (MQ=255)
                                                                                         gATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggcgg                                                 >  2:2373732/1‑148 (MQ=255)
                                                                                         gATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggcgg                                                 >  2:2525356/1‑148 (MQ=255)
                                                                                         gATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggcgg                                                 >  1:551918/1‑148 (MQ=255)
                                                                                         gATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggcgg                                                 >  2:2774197/1‑148 (MQ=255)
                                                                                         gATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCcggcgg                                                 >  1:1104660/1‑148 (MQ=255)
                                                                                           tGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGAt                                               <  2:1266917/148‑1 (MQ=255)
                                                                                           tGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGAt                                               <  2:1265673/148‑1 (MQ=255)
                                                                                           tGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGAt                                               >  1:2307443/1‑148 (MQ=255)
                                                                                              ggAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAg                                            <  1:3356523/148‑1 (MQ=255)
                                                                                               gAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGTGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGc                                           >  2:2403611/1‑148 (MQ=255)
                                                                                               gAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGc                                           >  1:1868439/1‑148 (MQ=255)
                                                                                               gAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGGGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGc                                           >  1:2437884/1‑148 (MQ=255)
                                                                                                   atCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGtt                                       <  2:1227486/148‑1 (MQ=255)
                                                                                                   atCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGtt                                       >  2:907742/1‑148 (MQ=255)
                                                                                                    tCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTc                                      <  2:14997/148‑1 (MQ=255)
                                                                                                       ggAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCg                                   >  1:1256253/1‑148 (MQ=255)
                                                                                                        gAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGt                                  >  1:761116/1‑148 (MQ=255)
                                                                                                         aGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGtt                                 <  1:426494/148‑1 (MQ=255)
                                                                                                           tCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCt                               >  2:18135/1‑148 (MQ=255)
                                                                                                           tCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCt                               >  2:742532/1‑148 (MQ=255)
                                                                                                           tCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCt                               <  1:1373113/148‑1 (MQ=255)
                                                                                                             ggTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGACTCTGCTCCGGCGGATAAGCTGTTCCCGTTCttt                             >  2:2239827/1‑148 (MQ=255)
                                                                                                             ggTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCttt                             >  2:2239956/1‑148 (MQ=255)
                                                                                                                    ccTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTa                      <  1:3187180/148‑1 (MQ=255)
                                                                                                                    ccTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTa                      >  1:465967/1‑148 (MQ=255)
                                                                                                                    ccTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTa                      <  1:3187223/148‑1 (MQ=255)
                                                                                                                         tAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTAccgcc                 >  1:1385848/1‑148 (MQ=255)
                                                                                                                          aaCGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTAccgccg                <  1:2494130/148‑1 (MQ=255)
                                                                                                                           aCGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCga               >  1:276009/1‑148 (MQ=255)
                                                                                                                             ggtGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCgaga             <  2:1706888/146‑1 (MQ=255)
                                                                                                                              ttGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGaa            <  1:2774207/148‑1 (MQ=255)
                                                                                                                                 cGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAatat         <  1:1558456/148‑1 (MQ=255)
                                                                                                                                   gCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATtg       <  1:1853322/148‑1 (MQ=255)
                                                                                                                                    cTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATtgt      >  2:1437849/1‑148 (MQ=255)
                                                                                                                                    cTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATtgt      <  2:2512444/148‑1 (MQ=255)
                                                                                                                                    cTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATtgt      <  2:2109440/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
TTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCA  >  CP009273/2574679‑2574962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCG  >  CP009273/2574679‑2574968
                                                                                                                                             |                                                                                                                                                    
TTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAG                                                                                                                                              <  SRR19973065.1453011/150‑1 (MQ=60)
  ACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCA                                                                                                                                            >  SRR19973065.14607/1‑150 (MQ=60)
  ACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGGGGAGGTGGAAGCA                                                                                                                                            >  SRR19973065.14606/1‑150 (MQ=60)
   CCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAG                                                                                                                                           >  SRR19973065.745534/1‑150 (MQ=60)
   CCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAG                                                                                                                                           >  SRR19973065.1547986/1‑150 (MQ=60)
   CCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAG                                                                                                                                           <  SRR19973065.1374934/150‑1 (MQ=60)
   CCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGGGGAGGTGGAAGCAG                                                                                                                                           >  SRR19973065.1547397/1‑150 (MQ=60)
   CCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTAGCGTGGAGGTGGAAGCAG                                                                                                                                           <  SRR19973065.227167/150‑1 (MQ=60)
    CCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGG                                                                                                                                          <  SRR19973065.1832394/150‑1 (MQ=60)
    CCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGG                                                                                                                                          <  SRR19973065.40335/150‑1 (MQ=60)
    CCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGG                                                                                                                                          <  SRR19973065.33215/150‑1 (MQ=60)
             GGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGA                                                                                                                                 >  SRR19973065.1838075/1‑150 (MQ=60)
              GCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGAT                                                                                                                                <  SRR19973065.3050136/150‑1 (MQ=60)
               CAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATT                                                                                                                               <  SRR19973065.1423523/150‑1 (MQ=60)
                   GAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGATGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTG                                                                                                                           <  SRR19973065.2445905/150‑1 (MQ=60)
                   GAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTG                                                                                                                           <  SRR19973065.2445884/150‑1 (MQ=60)
                    AAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGG                                                                                                                          <  SRR19973065.2738395/150‑1 (MQ=60)
                    AAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGG                                                                                                                          >  SRR19973065.2528229/1‑150 (MQ=60)
                    AAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGG                                                                                                                          >  SRR19973065.2528513/1‑150 (MQ=60)
                     AATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGT                                                                                                                         >  SRR19973065.1852101/1‑150 (MQ=60)
                     AATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGT                                                                                                                         >  SRR19973065.1852050/1‑150 (MQ=60)
                       TTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTAC                                                                                                                       <  SRR19973065.677963/150‑1 (MQ=60)
                        TAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACA                                                                                                                      <  SRR19973065.1409458/150‑1 (MQ=60)
                          GCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAG                                                                                                                    <  SRR19973065.930273/150‑1 (MQ=60)
                            AGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTA                                                                                                                  >  SRR19973065.2079982/1‑150 (MQ=60)
                            AGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTA                                                                                                                  >  SRR19973065.2123007/1‑150 (MQ=60)
                            AGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTA                                                                                                                  >  SRR19973065.2123084/1‑150 (MQ=60)
                             GGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTAT                                                                                                                 >  SRR19973065.963848/1‑150 (MQ=60)
                              GAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATG                                                                                                                <  SRR19973065.2404913/150‑1 (MQ=60)
                              GAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATG                                                                                                                >  SRR19973065.2616563/1‑150 (MQ=60)
                                GAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTT                                                                                                              >  SRR19973065.1780436/1‑150 (MQ=60)
                                  AGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGG                                                                                                            <  SRR19973065.1806255/150‑1 (MQ=60)
                                      CGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTG                                                                                                        >  SRR19973065.3396434/1‑150 (MQ=60)
                                         AATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGACGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAA                                                                                                     >  SRR19973065.3012134/1‑150 (MQ=60)
                                         AATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAA                                                                                                     >  SRR19973065.3012234/1‑150 (MQ=60)
                                           TGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGG                                                                                                   <  SRR19973065.112556/150‑1 (MQ=60)
                                           TGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGG                                                                                                   <  SRR19973065.3041634/150‑1 (MQ=60)
                                            GTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGG                                                                                                  >  SRR19973065.2150603/1‑150 (MQ=60)
                                               CGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCA                                                                                               >  SRR19973065.1778334/1‑150 (MQ=60)
                                                GCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAA                                                                                              <  SRR19973065.1687011/150‑1 (MQ=60)
                                                GCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAA                                                                                              <  SRR19973065.3435111/150‑1 (MQ=60)
                                                 CGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAAT                                                                                             <  SRR19973065.3135671/150‑1 (MQ=60)
                                                 CGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAAT                                                                                             <  SRR19973065.2561142/150‑1 (MQ=60)
                                                 CGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAAT                                                                                             <  SRR19973065.3135768/150‑1 (MQ=60)
                                                     GTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTC                                                                                         <  SRR19973065.3487563/150‑1 (MQ=60)
                                                     GTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTC                                                                                         <  SRR19973065.972693/150‑1 (MQ=60)
                                                     GTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTC                                                                                         <  SRR19973065.1263032/150‑1 (MQ=60)
                                                         CCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGA                                                                                     >  SRR19973065.1787435/1‑150 (MQ=60)
                                                             GCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGAC                                                                                 <  SRR19973065.943604/150‑1 (MQ=60)
                                                              CCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACG                                                                                >  SRR19973065.2870184/1‑150 (MQ=60)
                                                              CCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACG                                                                                >  SRR19973065.2870177/1‑150 (MQ=60)
                                                              CCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACG                                                                                >  SRR19973065.540672/1‑150 (MQ=60)
                                                              CCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACG                                                                                >  SRR19973065.542429/1‑150 (MQ=60)
                                                                CTCGACCGATATTTTCGACGCCCAGGATGAGGACTATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATTTTGGTCTGAAATGGGCAATTGTCGGGATGACGGG                                                                              >  SRR19973065.3134458/1‑150 (MQ=60)
                                                                CTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGG                                                                              >  SRR19973065.3388413/1‑150 (MQ=60)
                                                                CTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGG                                                                              >  SRR19973065.1280502/1‑150 (MQ=60)
                                                                CTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGG                                                                              >  SRR19973065.1280421/1‑150 (MQ=60)
                                                                CTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAAGTGTCGGGATGACGGG                                                                              >  SRR19973065.2163879/1‑150 (MQ=60)
                                                                CTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGGATGTTGGTCTGAAAGGGGCCATTGTCGGGATGACGGG                                                                              >  SRR19973065.40146/1‑150 (MQ=60)
                                                                 TCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGT                                                                             <  SRR19973065.1964648/150‑1 (MQ=60)
                                                                  CGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTT                                                                            <  SRR19973065.3505741/150‑1 (MQ=60)
                                                                  CGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTT                                                                            <  SRR19973065.3505706/150‑1 (MQ=60)
                                                                             TTCGTCGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCT                                                                 <  SRR19973065.3441033/150‑1 (MQ=60)
                                                                               CGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGC                                                               >  SRR19973065.2530598/1‑150 (MQ=60)
                                                                               CGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGC                                                               >  SRR19973065.2530464/1‑150 (MQ=60)
                                                                                    CCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCACCGG                                                          >  SRR19973065.1841002/1‑150 (MQ=60)
                                                                                      CAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCG                                                        >  SRR19973065.415389/1‑150 (MQ=60)
                                                                                       AGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGG                                                       >  SRR19973065.555462/1‑150 (MQ=60)
                                                                                       AGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGG                                                       >  SRR19973065.1111905/1‑150 (MQ=60)
                                                                                         GATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGAT                                                     >  SRR19973065.2296592/1‑150 (MQ=60)
                                                                                         GATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGAT                                                     >  SRR19973065.2321947/1‑150 (MQ=60)
                                                                                             AGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAACGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGC                                                 >  SRR19973065.553812/1‑150 (MQ=60)
                                                                                             AGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGC                                                 >  SRR19973065.2454485/1‑150 (MQ=60)
                                                                                             AGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGC                                                 >  SRR19973065.1880737/1‑150 (MQ=60)
                                                                                             AGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGC                                                 >  SRR19973065.1880129/1‑150 (MQ=60)
                                                                                             AGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGGGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGC                                                 >  SRR19973065.2453257/1‑150 (MQ=60)
                                                                                              GGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCT                                                <  SRR19973065.3377042/150‑1 (MQ=60)
                                                                                                  TATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTC                                            <  SRR19973065.1213656/150‑1 (MQ=60)
                                                                                                   ATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCC                                           <  SRR19973065.1958743/150‑1 (MQ=60)
                                                                                                     CGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCG                                         >  SRR19973065.1264525/1‑150 (MQ=60)
                                                                                                     CGGGAGTCGGTGTTGCCTTCTAAAGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGACGATTACTGGTAAAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCG                                         >  SRR19973065.1264083/1‑150 (MQ=60)
                                                                                                      GGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTACCGT                                        >  SRR19973065.766188/1‑150 (MQ=60)
                                                                                                         AGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCT                                     <  SRR19973065.429460/150‑1 (MQ=60)
                                                                                                           TCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTT                                   <  SRR19973065.1382173/150‑1 (MQ=60)
                                                                                                                  TGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTA                            >  SRR19973065.469029/1‑150 (MQ=60)
                                                                                                                  TGCCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTA                            >  SRR19973065.469174/1‑150 (MQ=60)
                                                                                                                    CCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACC                          <  SRR19973065.3206414/150‑1 (MQ=60)
                                                                                                                    CCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACC                          <  SRR19973065.3205870/150‑1 (MQ=60)
                                                                                                                    CCTTCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACC                          <  SRR19973065.3206457/150‑1 (MQ=60)
                                                                                                                       TCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCC                       >  SRR19973065.1394957/1‑150 (MQ=60)
                                                                                                                       TCTAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCC                       >  SRR19973065.1394954/1‑150 (MQ=60)
                                                                                                                         TAACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGA                     >  SRR19973065.277857/1‑150 (MQ=60)
                                                                                                                          AACGTTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAG                    <  SRR19973065.2509822/150‑1 (MQ=60)
                                                                                                                              TTGCGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATA                <  SRR19973065.2791434/150‑1 (MQ=60)
                                                                                                                              GTGCGGCTCGCGTGGAGGTGGAAGCAGGTAGTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATA                <  SRR19973065.2791424/150‑1 (MQ=60)
                                                                                                                                 CGGCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTG             <  SRR19973065.1568798/150‑1 (MQ=60)
                                                                                                                                   GCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTG           <  SRR19973065.1865525/150‑1 (MQ=60)
                                                                                                                                   GCTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTG           <  SRR19973065.3129151/150‑1 (MQ=60)
                                                                                                                                    CTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGG          <  SRR19973065.2761535/150‑1 (MQ=60)
                                                                                                                                    CTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGG          <  SRR19973065.564734/150‑1 (MQ=60)
                                                                                                                                    CTCGCGTGGAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGG          <  SRR19973065.345304/150‑1 (MQ=60)
                                                                                                                                            GAGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCG  >  SRR19973065.690596/1‑150 (MQ=60)
                                                                                                                                             |                                                                                                                                                    
TTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCGGATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCG  >  CP009273/2574679‑2574968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑