Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,606,516 G→T D420Y (GAC→TAC)  hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,606,5160GT99.6% 808.8 / NA 248D420Y (GAC→TAC) hyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (114/132);  total (115/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

GTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATT  >  CP009273/2606369‑2606662
                                                                                                                                                   |                                                                                                                                                  
  gtTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACaa                                                                                                                                                >  2:2583934/1‑150 (MQ=255)
   ttGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACaaa                                                                                                                                               <  1:901766/150‑1 (MQ=255)
    tGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAg                                                                                                                                              <  1:1135690/150‑1 (MQ=255)
    tGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAg                                                                                                                                              <  2:1263152/150‑1 (MQ=255)
      aTTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAggg                                                                                                                                            <  1:1831020/150‑1 (MQ=255)
      aTTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAggg                                                                                                                                            >  1:1637476/1‑150 (MQ=255)
       tttGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAgggg                                                                                                                                           <  1:1274026/150‑1 (MQ=255)
         tgtgGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCg                                                                                                                                         >  1:755365/1‑150 (MQ=255)
          gtggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGt                                                                                                                                        >  1:1880279/1‑150 (MQ=255)
          gtggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGt                                                                                                                                        >  1:1971185/1‑150 (MQ=255)
          gtggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGt                                                                                                                                        >  2:1761768/1‑150 (MQ=255)
              tgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTAc                                                                                                                                    >  2:893270/1‑150 (MQ=255)
                 aaCCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTgg                                                                                                                                 <  1:2712622/150‑1 (MQ=255)
                 aaCCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTgg                                                                                                                                 <  1:2429647/150‑1 (MQ=255)
                   ccGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTg                                                                                                                               <  2:2265780/150‑1 (MQ=255)
                    cGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGc                                                                                                                              <  2:2298931/150‑1 (MQ=255)
                        aCTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAtt                                                                                                                          <  2:1140453/150‑1 (MQ=255)
                         cTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTa                                                                                                                         >  2:74222/1‑150 (MQ=255)
                          tGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTaa                                                                                                                        <  1:2069371/150‑1 (MQ=255)
                          tGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTaa                                                                                                                        >  2:2445288/1‑150 (MQ=255)
                          tGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTaa                                                                                                                        >  2:1470186/1‑150 (MQ=255)
                           ggCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAAt                                                                                                                       <  1:3260317/150‑1 (MQ=255)
                           ggCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAAt                                                                                                                       <  2:1961558/150‑1 (MQ=255)
                           ggCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAAt                                                                                                                       >  2:3053890/1‑150 (MQ=255)
                            gCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATa                                                                                                                      >  2:3085353/1‑150 (MQ=255)
                            gCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATa                                                                                                                      >  2:2850211/1‑150 (MQ=255)
                             cAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAc                                                                                                                     <  1:337839/150‑1 (MQ=255)
                             cAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAc                                                                                                                     <  2:2156098/150‑1 (MQ=255)
                             cAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAc                                                                                                                     <  1:2165699/150‑1 (MQ=255)
                              aaaGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAcc                                                                                                                    <  1:465000/150‑1 (MQ=255)
                                agagGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCa                                                                                                                  <  1:3574687/150‑1 (MQ=255)
                                  agGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATc                                                                                                                <  2:3605868/150‑1 (MQ=255)
                                     tGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTg                                                                                                             >  2:240571/1‑150 (MQ=255)
                                      gATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTgg                                                                                                            <  1:1019827/150‑1 (MQ=255)
                                        tCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTc                                                                                                          >  1:3044943/1‑150 (MQ=255)
                                         cGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCg                                                                                                         <  2:1335925/150‑1 (MQ=255)
                                           ccAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGtt                                                                                                       <  1:420756/150‑1 (MQ=255)
                                               agCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGa                                                                                                   >  1:1555382/1‑150 (MQ=255)
                                                   aTCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAtt                                                                                               >  2:3033357/1‑150 (MQ=255)
                                                   aTCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAtt                                                                                               <  1:848396/150‑1 (MQ=255)
                                                    tCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTg                                                                                              >  1:3528822/1‑150 (MQ=255)
                                                      cATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGcc                                                                                            <  2:2508837/150‑1 (MQ=255)
                                                       aTCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCg                                                                                           >  1:622663/1‑150 (MQ=255)
                                                       aTCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCg                                                                                           <  1:2610219/150‑1 (MQ=255)
                                                              tAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATggcgg                                                                                    >  1:2937535/1‑150 (MQ=255)
                                                                gCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCa                                                                                  <  1:3481749/150‑1 (MQ=255)
                                                                 cccGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAAACCCGATGGCGGCAc                                                                                 >  1:1393743/1‑150 (MQ=255)
                                                                  ccGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACg                                                                                >  2:2610604/1‑150 (MQ=255)
                                                                       acgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgtt                                                                           <  2:3444380/150‑1 (MQ=255)
                                                                         gcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtc                                                                         <  1:2873518/150‑1 (MQ=255)
                                                                         gcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtc                                                                         <  1:971904/150‑1 (MQ=255)
                                                                           gacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtctc                                                                       <  1:2931419/150‑1 (MQ=255)
                                                                            acAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtctca                                                                      <  2:2143869/150‑2 (MQ=255)
                                                                            acAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCg                                                                      <  1:132986/150‑1 (MQ=255)
                                                                                gCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGa                                                                  <  1:1064883/150‑1 (MQ=255)
                                                                                  cGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaaa                                                                >  2:543306/1‑150 (MQ=255)
                                                                                  cGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaaa                                                                >  1:3231959/1‑150 (MQ=255)
                                                                                  cGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaaa                                                                >  1:107663/1‑150 (MQ=255)
                                                                                  cGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaaa                                                                >  2:2337078/1‑150 (MQ=255)
                                                                                    cTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAtt                                                              <  1:2445288/150‑1 (MQ=255)
                                                                                    cTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAtt                                                              <  1:1470186/150‑1 (MQ=255)
                                                                                         cAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGa                                                         >  2:3157817/1‑150 (MQ=255)
                                                                                          aaaaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAACTTGGCGAt                                                        >  2:2670757/1‑150 (MQ=255)
                                                                                          aaaaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGAt                                                        >  1:1741507/1‑150 (MQ=255)
                                                                                            aaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCt                                                      <  2:206260/150‑1 (MQ=255)
                                                                                             aTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTg                                                     <  2:1168442/150‑1 (MQ=255)
                                                                                             aTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTg                                                     <  1:2551586/150‑1 (MQ=255)
                                                                                                aaCTGAGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAATGGCGATCTGCCg                                                  >  2:187268/1‑150 (MQ=255)
                                                                                                 aCTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACATTGTTTCTCGATGAAATTGGCGATCTGCCGt                                                 >  2:464827/1‑150 (MQ=255)
                                                                                                    gcgcTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAg                                              <  2:3697153/150‑1 (MQ=255)
                                                                                                      gcTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGaa                                            >  2:1698242/1‑150 (MQ=255)
                                                                                                         gCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACtt                                         <  2:3326502/150‑1 (MQ=255)
                                                                                                          ccATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTc                                        >  1:1589776/1‑150 (MQ=255)
                                                                                                            aTCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAg                                      >  2:3784700/1‑150 (MQ=255)
                                                                                                            aTCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAg                                      <  2:3344689/150‑1 (MQ=255)
                                                                                                             tCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGc                                     <  2:3231959/150‑1 (MQ=255)
                                                                                                              ccccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGcc                                    >  2:3399715/1‑150 (MQ=255)
                                                                                                              ccccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGcc                                    <  2:1181899/150‑1 (MQ=255)
                                                                                                               cccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCt                                   <  1:2987062/150‑1 (MQ=255)
                                                                                                               cccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCt                                   >  2:172255/1‑150 (MQ=255)
                                                                                                                ccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTa                                  <  1:559158/150‑1 (MQ=255)
                                                                                                                 cGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTaa                                 >  1:480940/1‑150 (MQ=255)
                                                                                                                  gCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGAGGTCGTTTTGAAATTGCCGATGGCGGCACGATGTTTCTCGATGAAATTGGCGATCTGCCGATAGAACTTCAGCCTaaa                                >  2:158690/1‑150 (MQ=255)
                                                                                                                    cAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAAct                              <  2:3715793/150‑1 (MQ=255)
                                                                                                                     aGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActg                             >  1:2668485/1‑150 (MQ=255)
                                                                                                                     aGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActg                             <  2:406556/150‑1 (MQ=255)
                                                                                                                       tcttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgct                           <  2:2772042/150‑1 (MQ=255)
                                                                                                                       tcttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgct                           <  1:1370196/150‑1 (MQ=255)
                                                                                                                        cttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgctg                          >  2:1479465/1‑150 (MQ=255)
                                                                                                                         ttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACtgctgc                         <  1:3719436/150‑1 (MQ=255)
                                                                                                                             ggAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGt                     >  1:3678694/1‑150 (MQ=255)
                                                                                                                             ggAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGt                     >  1:1783944/1‑150 (MQ=255)
                                                                                                                                   tGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCa               >  1:3273458/1‑150 (MQ=255)
                                                                                                                                         aTTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCg         >  2:2955236/1‑150 (MQ=255)
                                                                                                                                         aTTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCg         >  2:61703/1‑150 (MQ=255)
                                                                                                                                          ttCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTgcg                        >  2:3574419/1‑134 (MQ=255)
                                                                                                                                          ttCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCgg        <  1:2354577/150‑1 (MQ=255)
                                                                                                                                            cGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGga      <  2:592665/150‑1 (MQ=255)
                                                                                                                                              gTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGgaga    >  2:1521409/1‑150 (MQ=255)
                                                                                                                                               tCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGAt   <  1:1239560/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATT  >  CP009273/2606369‑2606662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAG  >  CP009273/2606368‑2606665
                                                                                                                                                    |                                                                                                                                                     
GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTA                                                                                                                                                      >  SRR19973061.1485018/1‑150 (MQ=60)
 GTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTAC                                                                                                                                                     <  SRR19973061.423607/150‑1 (MQ=60)
  TGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACA                                                                                                                                                    <  SRR19973061.3270964/150‑1 (MQ=60)
    TTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAA                                                                                                                                                  <  SRR19973061.904085/150‑1 (MQ=60)
     TGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAG                                                                                                                                                 >  SRR19973061.407665/1‑150 (MQ=60)
     TGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAG                                                                                                                                                 <  SRR19973061.1138584/150‑1 (MQ=60)
      GATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGG                                                                                                                                                <  SRR19973061.131931/150‑1 (MQ=60)
       ATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGG                                                                                                                                               >  SRR19973061.1641576/1‑150 (MQ=60)
       ATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGG                                                                                                                                               <  SRR19973061.1835609/150‑1 (MQ=60)
        TTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGG                                                                                                                                              <  SRR19973061.1277165/150‑1 (MQ=60)
         TTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGC                                                                                                                                             <  SRR19973061.3191467/150‑1 (MQ=60)
          TGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCG                                                                                                                                            >  SRR19973061.757350/1‑150 (MQ=60)
           GTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGT                                                                                                                                           >  SRR19973061.1884955/1‑150 (MQ=60)
           GTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGT                                                                                                                                           >  SRR19973061.1976117/1‑150 (MQ=60)
           GTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATACGGTCATTACAAAGGGGCGT                                                                                                                                           >  SRR19973061.1975994/1‑150 (MQ=60)
            TGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTT                                                                                                                                          >  SRR19973061.496378/1‑150 (MQ=60)
            TGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTT                                                                                                                                          <  SRR19973061.2497480/150‑1 (MQ=60)
                 AAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTG                                                                                                                                     <  SRR19973061.2973039/150‑1 (MQ=60)
                  AACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGG                                                                                                                                    <  SRR19973061.2719608/150‑1 (MQ=60)
                  AACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGG                                                                                                                                    <  SRR19973061.2435851/150‑1 (MQ=60)
                  AACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACCTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGG                                                                                                                                    >  SRR19973061.2816583/1‑150 (MQ=60)
                      GGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAACCGCTGGTCAAAATCAACTGCGCTGCCAACCCCGCCAGTCTTCTGGACATTGAGTTATACGGTCAATCCAAAGGGGCGATTACTGGTGCG                                                                                                                                >  SRR19973061.340201/1‑150 (MQ=60)
                           TGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAA                                                                                                                           <  SRR19973061.2074571/150‑1 (MQ=60)
                           TGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAA                                                                                                                           <  SRR19973061.2074888/150‑1 (MQ=60)
                            GGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAAT                                                                                                                          <  SRR19973061.3268712/150‑1 (MQ=60)
                              CAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAC                                                                                                                        <  SRR19973061.2171138/150‑1 (MQ=60)
                              CAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAC                                                                                                                        <  SRR19973061.338726/150‑1 (MQ=60)
                               AAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACC                                                                                                                       <  SRR19973061.465463/150‑1 (MQ=60)
                               AAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACC                                                                                                                       <  SRR19973061.466263/150‑1 (MQ=60)
                               AAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACC                                                                                                                       <  SRR19973061.185377/150‑1 (MQ=60)
                                AAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCC                                                                                                                      >  SRR19973061.308102/1‑150 (MQ=60)
                                 AGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCA                                                                                                                     <  SRR19973061.3583849/150‑1 (MQ=60)
                                    GGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCG                                                                                                                  >  SRR19973061.594211/1‑150 (MQ=60)
                                       GATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGG                                                                                                               <  SRR19973061.1022424/150‑1 (MQ=60)
                                       GATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGG                                                                                                               <  SRR19973061.2928374/150‑1 (MQ=60)
                                         TCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTC                                                                                                             >  SRR19973061.3052815/1‑150 (MQ=60)
                                            CCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTT                                                                                                          <  SRR19973061.421896/150‑1 (MQ=60)
                                             CAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTT                                                                                                         >  SRR19973061.1151059/1‑150 (MQ=60)
                                              AGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTT                                                                                                        <  SRR19973061.2976344/150‑1 (MQ=60)
                                              AGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTT                                                                                                        >  SRR19973061.2061825/1‑150 (MQ=60)
                                                AGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGA                                                                                                      >  SRR19973061.3641235/1‑150 (MQ=60)
                                                AGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGA                                                                                                      >  SRR19973061.1559246/1‑150 (MQ=60)
                                                AGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGA                                                                                                      <  SRR19973061.1095747/150‑1 (MQ=60)
                                                   GATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAT                                                                                                   >  SRR19973061.211422/1‑150 (MQ=60)
                                                   GATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAT                                                                                                   >  SRR19973061.1588150/1‑150 (MQ=60)
                                                    ATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATT                                                                                                  >  SRR19973061.652000/1‑150 (MQ=60)
                                                    ATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATT                                                                                                  <  SRR19973061.850610/150‑1 (MQ=60)
                                                     TCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTG                                                                                                 >  SRR19973061.3537859/1‑150 (MQ=60)
                                                       CATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCC                                                                                               <  SRR19973061.1834738/150‑1 (MQ=60)
                                                       CATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCC                                                                                               <  SRR19973061.1834646/150‑1 (MQ=60)
                                                        ATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCG                                                                                              <  SRR19973061.1251705/150‑1 (MQ=60)
                                                        ATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCG                                                                                              >  SRR19973061.624255/1‑150 (MQ=60)
                                                        ATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCG                                                                                              <  SRR19973061.2616925/150‑1 (MQ=60)
                                                          CAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGAT                                                                                            <  SRR19973061.2333398/150‑1 (MQ=60)
                                                           AACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATG                                                                                           <  SRR19973061.2387813/150‑1 (MQ=60)
                                                           AACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATG                                                                                           <  SRR19973061.476013/150‑1 (MQ=60)
                                                               TAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGG                                                                                       >  SRR19973061.2945092/1‑150 (MQ=60)
                                                                AGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGC                                                                                      >  SRR19973061.3538201/1‑150 (MQ=60)
                                                                 GCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCA                                                                                     <  SRR19973061.3490657/150‑1 (MQ=60)
                                                                 GCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCA                                                                                     <  SRR19973061.3710683/150‑1 (MQ=60)
                                                                  CCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAAACCCGATGGCGGCAC                                                                                    >  SRR19973061.1397267/1‑150 (MQ=60)
                                                                    CGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGT                                                                                  >  SRR19973061.3703352/1‑150 (MQ=60)
                                                                    CGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGT                                                                                  <  SRR19973061.1033699/150‑1 (MQ=60)
                                                                      CGACGCGACAAGTCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTG                                                                                <  SRR19973061.2668875/150‑1 (MQ=60)
                                                                          GCGACCAGCCGCTGGTCAAACTCCCCTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCCTCGGGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTC                                                                            <  SRR19973061.3499374/150‑1 (MQ=60)
                                                                          GCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTC                                                                            <  SRR19973061.974374/150‑1 (MQ=60)
                                                                          GCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTC                                                                            <  SRR19973061.2880884/150‑1 (MQ=60)
                                                                            GACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTC                                                                          <  SRR19973061.2938946/150‑1 (MQ=60)
                                                                            GACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTC                                                                          <  SRR19973061.2938952/150‑1 (MQ=60)
                                                                            GACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTC                                                                          <  SRR19973061.2939012/150‑1 (MQ=60)
                                                                             ACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCG                                                                         <  SRR19973061.133414/150‑1 (MQ=60)
                                                                                 GCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGA                                                                     <  SRR19973061.1067621/150‑1 (MQ=60)
                                                                                 GCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGA                                                                     <  SRR19973061.1067958/150‑1 (MQ=60)
                                                                                   CGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAA                                                                   >  SRR19973061.747662/1‑150 (MQ=60)
                                                                                   CGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAA                                                                   <  SRR19973061.1899772/150‑1 (MQ=60)
                                                                                   CGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAA                                                                   >  SRR19973061.3240267/1‑150 (MQ=60)
                                                                                   CGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAA                                                                   >  SRR19973061.108021/1‑150 (MQ=60)
                                                                                    GCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAT                                                                  <  SRR19973061.1262097/150‑1 (MQ=60)
                                                                                    GCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGATGTTTCTCGATGAAAT                                                                  >  SRR19973061.1112445/1‑150 (MQ=60)
                                                                                     CTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATT                                                                 <  SRR19973061.2451524/150‑1 (MQ=60)
                                                                                     CTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATT                                                                 <  SRR19973061.1473335/150‑1 (MQ=60)
                                                                                     CTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATT                                                                 <  SRR19973061.1473879/150‑1 (MQ=60)
                                                                                     CTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATT                                                                 >  SRR19973061.931144/1‑150 (MQ=60)
                                                                                       GGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGG                                                               <  SRR19973061.3240791/150‑1 (MQ=60)
                                                                                        GTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGC                                                              <  SRR19973061.1367484/150‑1 (MQ=60)
                                                                                         TCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCG                                                             >  SRR19973061.2629900/1‑150 (MQ=60)
                                                                                          CAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGA                                                            <  SRR19973061.1370400/150‑1 (MQ=60)
                                                                                           AAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGAT                                                           >  SRR19973061.2447662/1‑150 (MQ=60)
                                                                                           AAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGAT                                                           >  SRR19973061.1745885/1‑150 (MQ=60)
                                                                                              ATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTG                                                        <  SRR19973061.2558086/150‑1 (MQ=60)
                                                                                              ATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTG                                                        <  SRR19973061.1518694/150‑1 (MQ=60)
                                                                                                  ACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGT                                                    >  SRR19973061.2490016/1‑150 (MQ=60)
                                                                                                      CGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGA                                                >  SRR19973061.1687952/1‑150 (MQ=60)
                                                                                                       GCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAA                                               >  SRR19973061.1577147/1‑150 (MQ=60)
                                                                                                         TGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACT                                             >  SRR19973061.3282546/1‑150 (MQ=60)
                                                                                                         TGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACT                                             <  SRR19973061.1462722/150‑1 (MQ=60)
                                                                                                         TGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACT                                             >  SRR19973061.1378728/1‑150 (MQ=60)
                                                                                                           CCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTC                                           >  SRR19973061.1602447/1‑150 (MQ=60)
                                                                                                           CCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTC                                           >  SRR19973061.1593726/1‑150 (MQ=60)
                                                                                                                CCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCT                                      <  SRR19973061.2994768/150‑1 (MQ=60)
                                                                                                                 CCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTA                                     <  SRR19973061.560628/150‑1 (MQ=60)
                                                                                                                  CGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAA                                    >  SRR19973061.482231/1‑150 (MQ=60)
                                                                                                                      AGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTG                                <  SRR19973061.3467749/150‑1 (MQ=60)
                                                                                                                      AGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTG                                >  SRR19973061.2675349/1‑150 (MQ=60)
                                                                                                                      AGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTATCGATGAAATTTGCGATCTGCCGTTATAAATTCAGCCTAAAATG                                >  SRR19973061.793691/1‑150 (MQ=60)
                                                                                                                        TCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCT                              <  SRR19973061.1373660/150‑1 (MQ=60)
                                                                                                                          TTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGC                            >  SRR19973061.1739787/1‑150 (MQ=60)
                                                                                                                          TTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGC                            <  SRR19973061.866323/150‑1 (MQ=60)
                                                                                                                          TTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGC                            <  SRR19973061.3728939/150‑1 (MQ=60)
                                                                                                                           tgtgctcttccgatctTTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCG                           <  SRR19973061.3583580/134‑1 (MQ=60)
                                                                                                                            CTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGC                          >  SRR19973061.489845/1‑150 (MQ=60)
                                                                                                                              GGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGT                        >  SRR19973061.1788414/1‑150 (MQ=60)
                                                                                                                              GGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGT                        >  SRR19973061.3688097/1‑150 (MQ=60)
                                                                                                                                AAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTAT                      <  SRR19973061.3392741/150‑1 (MQ=60)
                                                                                                                                AAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTAT                      <  SRR19973061.1764339/150‑1 (MQ=60)
                                                                                                                                   GTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGC                   >  SRR19973061.305251/1‑150 (MQ=60)
                                                                                                                                    TGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCA                  >  SRR19973061.3281890/1‑150 (MQ=60)
                                                                                                                                    TGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCA                  >  SRR19973061.3281889/1‑150 (MQ=60)
                                                                                                                                        TTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAG              <  SRR19973061.2590552/150‑1 (MQ=60)
                                                                                                                                           TTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGG           <  SRR19973061.2360562/150‑1 (MQ=60)
                                                                                                                                           TTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGG           <  SRR19973061.2361970/150‑1 (MQ=60)
                                                                                                                                              GGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAG        >  SRR19973061.270897/1‑150 (MQ=60)
                                                                                                                                                TCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGAT      <  SRR19973061.1242639/150‑1 (MQ=60)
                                                                                                                                                 CATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATT     >  SRR19973061.888810/1‑150 (MQ=60)
                                                                                                                                                  ATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATTAAATTGGCGATCTGCCGTTAAAACATCAGCCAAAACTGCAGCGCGTATTGCAGGAGCGGGAGATTG    >  SRR19973061.25875/1‑150 (MQ=60)
                                                                                                                                                  ATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTG    >  SRR19973061.2106879/1‑150 (MQ=60)
                                                                                                                                                    TACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAG  >  SRR19973061.579056/1‑150 (MQ=60)
                                                                                                                                                    TACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAG  >  SRR19973061.3135801/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAG  >  CP009273/2606368‑2606665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑