Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,606,516 G→T 100% D420Y (GAC→TAC)  hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,606,5160GT100.0% 794.8 / ‑6.7 239D420Y (GAC→TAC) hyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (111/127);  total (112/127)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

TGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGA  >  CP009273/2606370‑2606660
                                                                                                                                                  |                                                                                                                                                
tgtTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTAc                                                                                                                                                >  2:55925/1‑149 (MQ=255)
   tGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACaaa                                                                                                                                             <  2:3862546/149‑1 (MQ=255)
      tttGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAggg                                                                                                                                          >  1:3878972/1‑149 (MQ=255)
        tgtgGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGc                                                                                                                                        <  2:238680/149‑1 (MQ=255)
         gtggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCg                                                                                                                                       <  2:1913320/149‑1 (MQ=255)
         gtggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCg                                                                                                                                       >  2:3685545/1‑149 (MQ=255)
           ggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGtt                                                                                                                                     <  1:2833171/149‑1 (MQ=255)
           ggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGtt                                                                                                                                     <  1:763335/149‑1 (MQ=255)
             tgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTa                                                                                                                                   >  1:757284/1‑149 (MQ=255)
             tgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTa                                                                                                                                   >  1:2111007/1‑149 (MQ=255)
              gAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTAc                                                                                                                                  >  2:919775/1‑149 (MQ=255)
                aaCCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTg                                                                                                                                >  2:1531028/1‑149 (MQ=255)
                aaCCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTg                                                                                                                                <  1:2133587/149‑1 (MQ=255)
                  ccGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGt                                                                                                                              >  2:3323972/1‑149 (MQ=255)
                    ggAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGc                                                                                                                            >  1:2314226/1‑149 (MQ=255)
                       aCTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAt                                                                                                                         >  1:987490/1‑149 (MQ=255)
                        cTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAtt                                                                                                                        >  2:16992/1‑149 (MQ=255)
                         tGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTa                                                                                                                       <  2:3503982/149‑1 (MQ=255)
                          ggCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTaa                                                                                                                      <  2:2785091/149‑1 (MQ=255)
                          ggCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTaa                                                                                                                      <  2:1928691/149‑1 (MQ=255)
                            cAAAGAGGTGATCGCCAGAGCGATCCATCATCTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATa                                                                                                                    <  1:2533436/149‑1 (MQ=255)
                            cAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATa                                                                                                                    >  1:2817706/1‑149 (MQ=255)
                              aaGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAcc                                                                                                                  >  1:1838430/1‑149 (MQ=255)
                               agagGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAccc                                                                                                                 >  1:1621136/1‑149 (MQ=255)
                                gagGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCa                                                                                                                <  1:1684675/149‑1 (MQ=255)
                                gagGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCa                                                                                                                <  2:2884457/149‑1 (MQ=255)
                                gagGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCa                                                                                                                <  1:3729980/149‑1 (MQ=255)
                                  ggTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATc                                                                                                              <  1:3230281/149‑1 (MQ=255)
                                       tCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGt                                                                                                         <  2:3588341/149‑1 (MQ=255)
                                          ccAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGt                                                                                                      >  1:1435463/1‑149 (MQ=255)
                                            agagCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGgtt                                                                                                    >  1:139392/1‑149 (MQ=255)
                                               gCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGa                                                                                                 >  2:754983/1‑149 (MQ=255)
                                                cGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGaa                                                                                                >  2:1257966/1‑149 (MQ=255)
                                                 gATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGaaa                                                                                               <  1:1086526/149‑1 (MQ=255)
                                                 gATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGaaa                                                                                               >  1:3826788/1‑149 (MQ=255)
                                                 gATCCATCAACGTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGaaa                                                                                               <  2:632131/149‑1 (MQ=255)
                                                  aTCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAt                                                                                              <  2:233909/149‑1 (MQ=255)
                                                  aTCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAt                                                                                              >  2:77380/1‑149 (MQ=255)
                                                   tCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAtt                                                                                             <  1:3685545/149‑1 (MQ=255)
                                                   tCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAtt                                                                                             <  1:2907942/149‑1 (MQ=255)
                                                     cATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGc                                                                                           <  2:352552/149‑1 (MQ=255)
                                                      aTCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGcc                                                                                          <  2:3276784/149‑1 (MQ=255)
                                                       tCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCg                                                                                         >  2:1798641/1‑149 (MQ=255)
                                                        cAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGa                                                                                        <  1:3744600/149‑1 (MQ=255)
                                                           cTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATgg                                                                                     <  2:345447/149‑1 (MQ=255)
                                                            ttAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATggc                                                                                    <  1:482291/149‑1 (MQ=255)
                                                             tAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATggcg                                                                                   <  2:2746779/149‑1 (MQ=255)
                                                                 ccGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCAc                                                                               <  2:2405040/149‑1 (MQ=255)
                                                                 ccGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCAc                                                                               <  2:3245770/149‑1 (MQ=255)
                                                                      acgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgt                                                                          <  2:2204739/149‑1 (MQ=255)
                                                                      acgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgt                                                                          <  2:1217230/149‑1 (MQ=255)
                                                                      acgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgt                                                                          <  1:225148/149‑1 (MQ=255)
                                                                      acgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACgttgt                                                                          >  1:426260/1‑149 (MQ=255)
                                                                         cgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtc                                                                       <  1:404572/149‑1 (MQ=255)
                                                                           acAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTtctc                                                                     >  1:2620011/1‑149 (MQ=255)
                                                                             aaGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTCTCGa                                                                   >  1:3020014/1‑149 (MQ=255)
                                                                             aaGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGGTGTTTCTCGa                                                                   >  1:1502395/1‑149 (MQ=255)
                                                                              aGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGAt                                                                  >  2:3207593/1‑149 (MQ=255)
                                                                              aGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGAt                                                                  >  2:1155500/1‑149 (MQ=255)
                                                                              aGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGAt                                                                  >  2:2674062/1‑149 (MQ=255)
                                                                               gCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATg                                                                 <  1:1147519/149‑1 (MQ=255)
                                                                               gCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATg                                                                 <  1:2687952/149‑1 (MQ=255)
                                                                                        cAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCTTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCg                                                        <  2:3477860/149‑1 (MQ=255)
                                                                                         aaaaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGa                                                       <  2:1671910/149‑1 (MQ=255)
                                                                                           aaTCAACTGCGCGGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATc                                                     <  2:1859190/149‑1 (MQ=255)
                                                                                              cAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGc                                                  <  1:3304560/149‑1 (MQ=255)
                                                                                               aaCTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGcc                                                 <  2:3204072/149‑1 (MQ=255)
                                                                                                   gcgcTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTa                                             <  1:1521376/149‑1 (MQ=255)
                                                                                                      cTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGaa                                          <  1:1988164/149‑1 (MQ=255)
                                                                                                      cTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAATGGCGATCTGCCGTTAGaa                                          >  2:801012/1‑149 (MQ=255)
                                                                                                       tGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTTCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAAc                                         <  1:82572/149‑1 (MQ=255)
                                                                                                       tGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTTGCGATCTGCCGTTAGAAc                                         >  1:1100301/1‑149 (MQ=255)
                                                                                                        gCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGTAATTGGCGATCTGCCGTTAGAACt                                        >  2:1165396/1‑149 (MQ=255)
                                                                                                        gCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAAcg                                        >  2:2976780/1‑148 (MQ=255)
                                                                                                        gCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACt                                        >  2:3273628/1‑149 (MQ=255)
                                                                                                           aTCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCa                                     <  1:3388755/149‑1 (MQ=255)
                                                                                                                  ccAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTaaa                              >  2:41329/1‑149 (MQ=255)
                                                                                                                   cAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAAc                             >  2:4036318/1‑149 (MQ=255)
                                                                                                                   cAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTGACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAAc                             <  1:2979873/149‑1 (MQ=255)
                                                                                                                     gTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAATTTCCGATGGCGGCACCGTGTTTCTCGATGAAATTTTCGATCTGCCGTTAGTAAATAAGCCTaaaaac                           >  1:2782464/1‑146 (MQ=255)
                                                                                                                     gTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActg                           <  2:514339/149‑1 (MQ=255)
                                                                                                                      tcttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgc                          <  2:139404/149‑1 (MQ=255)
                                                                                                                         tctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACtgctgc                       <  2:3428722/149‑1 (MQ=255)
                                                                                                                         tctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACtgctgc                       <  2:3428862/149‑1 (MQ=255)
                                                                                                                           tGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTgcgn                     <  1:1941406/149‑2 (MQ=255)
                                                                                                                             gAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATTGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGGGCGt                   >  1:3849301/1‑149 (MQ=255)
                                                                                                                             gAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGt                   >  1:3046857/1‑149 (MQ=255)
                                                                                                                              aaaGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTa                  <  2:1872491/149‑1 (MQ=255)
                                                                                                                                  tGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGc              <  1:118546/149‑1 (MQ=255)
                                                                                                                                   gAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCa             <  2:2925039/149‑1 (MQ=255)
                                                                                                                                     gTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAgg           <  1:3717097/149‑1 (MQ=255)
                                                                                                                                     gTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGCTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAgg           >  2:1065393/1‑149 (MQ=255)
                                                                                                                                      ttATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGa          >  2:2049966/1‑149 (MQ=255)
                                                                                                                                       tATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAATTTCCGATGGCGGCACGTTGTTTATCGATGAAATTTGCGATCTGCCGGTTGAAATTCAGCCTAAACAGCTGCGCGTATTTCAGGAg         >  1:3548837/1‑149 (MQ=255)
                                                                                                                                         ttCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTGTCGATGAAATTTGCGATATGCCGGTAGAAATTAAGCCAAAAAATCTGCGCGGATTTCAGGAGCg       >  1:409261/1‑149 (MQ=255)
                                                                                                                                            ggTCATTACCAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGga    <  2:3926971/149‑1 (MQ=255)
                                                                                                                                            ggTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATTGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTTCAGGAGCGGga    >  1:682674/1‑149 (MQ=255)
                                                                                                                                            ggTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGga    <  2:2314226/149‑1 (MQ=255)
                                                                                                                                            ggTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGga    <  2:700172/149‑1 (MQ=255)
                                                                                                                                             gTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTAAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGgag   >  2:2893493/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGA  >  CP009273/2606370‑2606660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAG  >  CP009273/2606368‑2606665
                                                                                                                                                    |                                                                                                                                                     
GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTA                                                                                                                                                      <  SRR19973068.2948561/150‑1 (MQ=60)
GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTA                                                                                                                                                      <  SRR19973068.3993384/150‑1 (MQ=60)
 GTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTAC                                                                                                                                                     <  SRR19973068.461025/150‑1 (MQ=60)
  TGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACA                                                                                                                                                    >  SRR19973068.2928725/1‑150 (MQ=60)
  TGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACA                                                                                                                                                    <  SRR19973068.1135414/150‑1 (MQ=60)
  TGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACA                                                                                                                                                    >  SRR19973068.2929043/1‑150 (MQ=60)
     TGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAG                                                                                                                                                 >  SRR19973068.1588319/1‑150 (MQ=60)
       ATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGG                                                                                                                                               >  SRR19973068.3902395/1‑150 (MQ=60)
             GGTGAACCCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCACCTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTT                                                                                                                                         <  SRR19973068.763392/150‑1 (MQ=60)
             GGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTT                                                                                                                                         <  SRR19973068.2850954/150‑1 (MQ=60)
             GGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTT                                                                                                                                         <  SRR19973068.2850824/150‑1 (MQ=60)
             GGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTT                                                                                                                                         <  SRR19973068.2850812/150‑1 (MQ=60)
             GGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTT                                                                                                                                         <  SRR19973068.768480/150‑1 (MQ=60)
             GGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTT                                                                                                                                         <  SRR19973068.800980/150‑1 (MQ=60)
              GTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTA                                                                                                                                        >  SRR19973068.762373/1‑150 (MQ=60)
              GTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTA                                                                                                                                        >  SRR19973068.2124767/1‑150 (MQ=60)
              GTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTA                                                                                                                                        <  SRR19973068.429598/150‑1 (MQ=60)
              GTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTA                                                                                                                                        >  SRR19973068.2124789/1‑150 (MQ=60)
              GTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTGTA                                                                                                                                        >  SRR19973068.762873/1‑150 (MQ=60)
                  AACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGG                                                                                                                                    <  SRR19973068.2147543/150‑1 (MQ=60)
                   ACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGT                                                                                                                                   <  SRR19973068.1428175/150‑1 (MQ=60)
                   ACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGT                                                                                                                                   <  SRR19973068.1951701/150‑1 (MQ=60)
                     CGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGC                                                                                                                                 >  SRR19973068.2329156/1‑150 (MQ=60)
                     CGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGC                                                                                                                                 >  SRR19973068.2328943/1‑150 (MQ=60)
                        AACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAT                                                                                                                              >  SRR19973068.993803/1‑150 (MQ=60)
                        AACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAT                                                                                                                              >  SRR19973068.993988/1‑150 (MQ=60)
                             GCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATA                                                                                                                         >  SRR19973068.2834580/1‑150 (MQ=60)
                             GCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATA                                                                                                                         >  SRR19973068.2835231/1‑150 (MQ=60)
                              CAAAGAGGTGATCGCCAGAGCGATCCATCATCTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAC                                                                                                                        <  SRR19973068.2549533/150‑1 (MQ=60)
                              CAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAC                                                                                                                        <  SRR19973068.2127101/150‑1 (MQ=60)
                               AAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACC                                                                                                                       >  SRR19973068.1850554/1‑150 (MQ=60)
                                AAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCC                                                                                                                      >  SRR19973068.1631723/1‑150 (MQ=60)
                                  GAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCAT                                                                                                                    <  SRR19973068.3752366/150‑1 (MQ=60)
                                  GAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCAT                                                                                                                    <  SRR19973068.1695505/150‑1 (MQ=60)
                                    GGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCG                                                                                                                  <  SRR19973068.3161028/150‑1 (MQ=60)
                                    GGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCG                                                                                                                  <  SRR19973068.3250134/150‑1 (MQ=60)
                                           GCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGT                                                                                                           >  SRR19973068.1444846/1‑150 (MQ=60)
                                             CAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTT                                                                                                         >  SRR19973068.3407191/1‑150 (MQ=60)
                                             CAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGGTT                                                                                                         >  SRR19973068.140635/1‑150 (MQ=60)
                                                AGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGA                                                                                                      <  SRR19973068.3591035/150‑1 (MQ=60)
                                                  CGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTGTTGAAA                                                                                                    >  SRR19973068.3849840/1‑150 (MQ=60)
                                                   GATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAT                                                                                                   <  SRR19973068.3755829/150‑1 (MQ=60)
                                                   GATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAT                                                                                                   <  SRR19973068.1093732/150‑1 (MQ=60)
                                                   GATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAT                                                                                                   <  SRR19973068.3756027/150‑1 (MQ=60)
                                                   GATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAA                                                                                                   >  SRR19973068.2462572/1‑150 (MQ=60)
                                                   GATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTGTACTGGTGCGATTAATACCCATCGTGGTCGTGTTGTAAT                                                                                                   >  SRR19973068.1902852/1‑150 (MQ=60)
                                                     TCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTG                                                                                                 <  SRR19973068.3707522/150‑1 (MQ=60)
                                                     TCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTG                                                                                                 <  SRR19973068.3707774/150‑1 (MQ=60)
                                                     TCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTG                                                                                                 <  SRR19973068.2926034/150‑1 (MQ=60)
                                                        ATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCG                                                                                              <  SRR19973068.3046127/150‑1 (MQ=60)
                                                        ATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCG                                                                                              <  SRR19973068.3046214/150‑1 (MQ=60)
                                                          CAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGAT                                                                                            <  SRR19973068.3767019/150‑1 (MQ=60)
                                                          CAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGAT                                                                                            <  SRR19973068.1710877/150‑1 (MQ=60)
                                                          CAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGAT                                                                                            <  SRR19973068.3031766/150‑1 (MQ=60)
                                                           AACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATG                                                                                           <  SRR19973068.3468867/150‑1 (MQ=60)
                                                              TTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCG                                                                                        <  SRR19973068.1392429/150‑1 (MQ=60)
                                                              TTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCG                                                                                        <  SRR19973068.485685/150‑1 (MQ=60)
                                                               TAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGG                                                                                       >  SRR19973068.3875402/1‑150 (MQ=60)
                                                                     GCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTT                                                                                 <  SRR19973068.2656718/150‑1 (MQ=60)
                                                                      CGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTG                                                                                >  SRR19973068.2550084/1‑150 (MQ=60)
                                                                      CGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTG                                                                                <  SRR19973068.3791105/150‑1 (MQ=60)
                                                                       GACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGT                                                                               >  SRR19973068.429274/1‑150 (MQ=60)
                                                                        ACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTT                                                                              <  SRR19973068.226851/150‑1 (MQ=60)
                                                                        ACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTT                                                                              <  SRR19973068.226825/150‑1 (MQ=60)
                                                                         CGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTT                                                                             <  SRR19973068.1956808/150‑1 (MQ=60)
                                                                           CGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCT                                                                           <  SRR19973068.407477/150‑1 (MQ=60)
                                                                           CGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCT                                                                           <  SRR19973068.407413/150‑1 (MQ=60)
                                                                            GACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTC                                                                          >  SRR19973068.2636503/1‑150 (MQ=60)
                                                                              CAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTCTCGA                                                                        >  SRR19973068.3038604/1‑150 (MQ=60)
                                                                              CAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTCTCGA                                                                        >  SRR19973068.2600921/1‑150 (MQ=60)
                                                                              CAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGGTGTTTCTCGA                                                                        >  SRR19973068.1512273/1‑150 (MQ=60)
                                                                               AAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGGAATTTCCGATGGCGGCACGTTGTTTATCGAT                                                                       >  SRR19973068.1101959/1‑150 (MQ=60)
                                                                               AAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGAT                                                                       >  SRR19973068.2039256/1‑150 (MQ=60)
                                                                                 GCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGA                                                                     <  SRR19973068.1155201/150‑1 (MQ=60)
                                                                                 GCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGA                                                                     <  SRR19973068.1155207/150‑1 (MQ=60)
                                                                                 GCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGA                                                                     <  SRR19973068.1155229/150‑1 (MQ=60)
                                                                                 GCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGA                                                                     <  SRR19973068.2704681/150‑1 (MQ=60)
                                                                                   CGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTATCGATGAAA                                                                   >  SRR19973068.1631074/1‑150 (MQ=60)
                                                                                             AATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCT                                                         <  SRR19973068.3744412/150‑1 (MQ=60)
                                                                                                CAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCC                                                      <  SRR19973068.3324782/150‑1 (MQ=60)
                                                                                                    TGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTGCCGATGGCGGCACGTTGTTTGTCGATGAAATTGGCGATCTGCCGTTT                                                  >  SRR19973068.1522497/1‑150 (MQ=60)
                                                                                                     GCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAG                                                 <  SRR19973068.1531380/150‑1 (MQ=60)
                                                                                                        CTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTTGCGATCTGCCGTTAGAAC                                              >  SRR19973068.1107629/1‑150 (MQ=60)
                                                                                                        CTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAAC                                              <  SRR19973068.2001288/150‑1 (MQ=60)
                                                                                                         TGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTTCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACT                                             <  SRR19973068.83446/150‑1 (MQ=60)
                                                                                                            CATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTCTCGATGAAATTTGCGATCTGCCGTTAGAACTTCA                                          >  SRR19973068.3854594/1‑150 (MQ=60)
                                                                                                            CATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCCCGTTGTTTCTCGGTGAAATTTGCGATCTTCCGTTAGAAaatca                                          >  SRR19973068.1450498/1‑145 (MQ=60)
                                                                                                            CATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTGTTGAAATTGCCGATGGCGGCCCGTTGTTTGTCGATGAAATTGGCGATCTGGCGGGAGAACTTCA                                          >  SRR19973068.3854651/1‑150 (MQ=60)
                                                                                                             ATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAG                                         <  SRR19973068.3409299/150‑1 (MQ=60)
                                                                                                                CCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCT                                      <  SRR19973068.40499/150‑1 (MQ=60)
                                                                                                                    CCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAAC                                  <  SRR19973068.3386318/150‑1 (MQ=60)
                                                                                                                     CAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTGACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACT                                 <  SRR19973068.2998315/150‑1 (MQ=60)
                                                                                                                      AGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAATTTCCGATGGCGGCACCGTGTTTCTCGATGAAATTTTCGATCTGCCGTTAGtaaataagcctaaaaac                                >  SRR19973068.2799676/1‑133 (MQ=60)
                                                                                                                         CTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTTCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTAAGCCTAAACTGCGG                             >  SRR19973068.2825370/1‑150 (MQ=60)
                                                                                                                             TGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGNG                         <  SRR19973068.1954314/150‑1 (MQ=60)
                                                                                                                             TGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCG                         >  SRR19973068.4070729/1‑150 (MQ=60)
                                                                                                                              GGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAATTTCCGATGGCGGCACGTTTTTTATCGATGtaatttgggatatggcggtagtacataagccgaaaaatctgcgcgg                        >  SRR19973068.2782851/1‑104 (MQ=60)
                                                                                                                              GGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATTGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGGGCGT                        >  SRR19973068.3872534/1‑150 (MQ=60)
                                                                                                                              GGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGT                        >  SRR19973068.3065534/1‑150 (MQ=60)
                                                                                                                                    TGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAATTTCCCATGGCGGCACGGTGTTTGTCGATTAAAATTGCGATATGCCGTTATAACATAAGCCTAAAAAGCAGCGCGTATTGCA                  >  SRR19973068.1475965/1‑150 (MQ=60)
                                                                                                                                    TGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCA                  <  SRR19973068.3312760/150‑1 (MQ=60)
                                                                                                                                    TGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCA                  <  SRR19973068.119671/150‑1 (MQ=60)
                                                                                                                                     GAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAG                 >  SRR19973068.1692351/1‑150 (MQ=60)
                                                                                                                                      AGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACATCAGCCTAAACTGCTGCGCGTATTGCAGG                >  SRR19973068.4127393/1‑150 (MQ=60)
                                                                                                                                       GTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGA               <  SRR19973068.3739428/150‑1 (MQ=60)
                                                                                                                                       GTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGA               <  SRR19973068.3739149/150‑1 (MQ=60)
                                                                                                                                        TTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAATTTCCGATGGCGGCACGTTGTTTATCGATGAAATTTGCGATCTGCCGGTTGAAATTCAGCCTAAACAGCTGCGCGTATTTCAGGAG              >  SRR19973068.3570403/1‑150 (MQ=60)
                                                                                                                                          ATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTTCCGATTGCGGCACGGTTTTTCTCGATGAAATTGGCGCTCTGCCGTGAGAACTTCAGCCTAAAAAGCTGCGCGTATTTCAGGAGCG            >  SRR19973068.2152617/1‑150 (MQ=60)
                                                                                                                                          ATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTGTCGATGAAATTTGCGATATGCCGGTAGAAattaagccaaaaaatctgcgcggatttcaggagcg            >  SRR19973068.412136/1‑115 (MQ=60)
                                                                                                                                          ATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGGTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAAAATAAGCCTAAACAGCGGCGCGTATTTCAGGAGCG            >  SRR19973068.57393/1‑150 (MQ=60)
                                                                                                                                             CGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATTGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTTCAGGAGCGGGA         >  SRR19973068.687001/1‑150 (MQ=60)
                                                                                                                                             CGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTTCAGGAGCGGGA         >  SRR19973068.689019/1‑150 (MQ=60)
                                                                                                                                                TCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGAT      <  SRR19973068.538995/150‑1 (MQ=60)
                                                                                                                                                  ATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTATCGATGAAAATTGCGAACTGCCGTTAGAAATTCAGCCTAAACAGCTGCGCGTATTTCAGGAGCGGGAGATTT    >  SRR19973068.2942506/1‑150 (MQ=60)
                                                                                                                                                  ATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCcgcaaggtgtttatagattaaaattgcgatcttcccatataacttaaaacaaaaatccttcgcgtctttcaggagcgggagattg    >  SRR19973068.2942535/1‑65 (MQ=60)
                                                                                                                                                  ATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGGTGTTTCTCGATGAAAATGGCGATCTTCCGTTAGAACTTCAGCCTAAAAATCTGCGCGTATTTCAGGAGCGGGAGATTG    >  SRR19973068.2942504/1‑150 (MQ=60)
                                                                                                                                                    TACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCCATGGCGGCACCTTGTTTCTAGATGTAATTTGCGATCTGCCCTTAGAAATTCATCCTAAACTTCTGCGCGTATTTCAGGAGCGGGAGATTGTG  >  SRR19973068.186139/1‑150 (MQ=60)
                                                                                                                                                    TACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAG  <  SRR19973068.3476178/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GGTGTTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGATTGAG  >  CP009273/2606368‑2606665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑