Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,639,116	C→A	A523A (GCG→GCT)	pbpC ←	penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,639,116	0	C	A	99.6%	749.9 / NA	239	A523A (GCG→GCT)	pbpC	penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (136/102); total (136/103)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

GGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCG  >  CP009273/2638970‑2639262

ggCAGATTCGCACTTCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGcc                                                                                                                                                  <  2:3370880/150‑1 (MQ=255)

 gCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGccc                                                                                                                                                 <  1:327879/150‑1 (MQ=255)

 gCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGccc                                                                                                                                                 <  2:1152078/150‑1 (MQ=255)

  cAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCa                                                                                                                                                >  2:778636/1‑150 (MQ=255)

    gATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAgg                                                                                                                                              <  1:1301076/150‑1 (MQ=255)

        cGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTc                                                                                                                                          >  2:1408289/1‑150 (MQ=255)

           aCTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACg                                                                                                                                       >  2:3591120/1‑150 (MQ=255)

           aCTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACg                                                                                                                                       >  2:1548135/1‑150 (MQ=255)

                 cgACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGcc                                                                                                                                 <  2:1491854/150‑1 (MQ=255)

                 cgACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGcc                                                                                                                                 >  1:1656217/1‑150 (MQ=255)

                  gACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCa                                                                                                                                <  2:3075941/150‑1 (MQ=255)

                    cAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATa                                                                                                                              <  1:1075891/150‑1 (MQ=255)

                    cAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATa                                                                                                                              >  1:3181575/1‑150 (MQ=255)

                     aGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAg                                                                                                                             <  2:3423371/150‑1 (MQ=255)

                       tAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCt                                                                                                                           <  1:3332779/150‑1 (MQ=255)

                       tAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCt                                                                                                                           <  2:375216/150‑1 (MQ=255)

                        aaGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTg                                                                                                                          >  2:516526/1‑150 (MQ=255)

                         aGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTgg                                                                                                                         >  1:2284020/1‑150 (MQ=255)

                          gATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGt                                                                                                                        <  1:2085097/150‑1 (MQ=255)

                          gATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACAAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCACTAGCCCAGGCGTCACGATAGCCATAGCTGGt                                                                                                                        >  2:750586/1‑150 (MQ=255)

                              ttattGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGccc                                                                                                                    <  2:1935794/150‑1 (MQ=255)

                                attGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGt                                                                                                                  <  2:2794729/150‑1 (MQ=255)

                                 ttGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGtt                                                                                                                 <  1:3156970/150‑1 (MQ=255)

                                 ttGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGtt                                                                                                                 >  1:692089/1‑150 (MQ=255)

                                  tGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGttt                                                                                                                >  1:783248/1‑150 (MQ=255)

                                   gACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAAAGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGtttt                                                                                                               >  1:3692533/1‑150 (MQ=255)

                                    aCCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTc                                                                                                              <  2:2366055/150‑1 (MQ=255)

                                     ccTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTcc                                                                                                             <  1:13957/150‑1 (MQ=255)

                                     ccTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTcc                                                                                                             <  1:1925544/150‑1 (MQ=255)

                                      cTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCa                                                                                                            >  1:2358215/1‑150 (MQ=255)

                                        gATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATg                                                                                                          >  2:1630597/1‑150 (MQ=255)

                                            caacaaTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAg                                                                                                      >  1:3496755/1‑150 (MQ=255)

                                             aacaaTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGt                                                                                                     >  1:3567028/1‑150 (MQ=255)

                                             aacaaTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGt                                                                                                     >  1:2760596/1‑150 (MQ=255)

                                             aacaaTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGt                                                                                                     >  1:2624238/1‑150 (MQ=255)

                                                aaTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑ggg                                                                                                 <  2:801624/150‑1 (MQ=255)

                                                aaTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑ggg                                                                                                 >  1:1913527/1‑150 (MQ=255)

                                                 aTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑gggg                                                                                                <  2:482828/150‑1 (MQ=255)

                                                   ggTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCg                                                                                              <  1:288443/150‑1 (MQ=255)

                                                     tACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGAc                                                                                            <  2:1165695/150‑1 (MQ=255)

                                                          cACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGAcgcgcg                                                                                       >  1:46841/1‑150 (MQ=255)

                                                           aCTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCgg                                                                                      <  1:1636552/150‑1 (MQ=255)

                                                            cTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGc                                                                                     >  2:3074343/1‑150 (MQ=255)

                                                             tGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCa                                                                                    >  1:3203331/1‑150 (MQ=255)

                                                                 aaaGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGc                                                                                >  2:393819/1‑150 (MQ=255)

                                                                  aaGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCa                                                                               <  1:2524198/150‑1 (MQ=255)

                                                                   aGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCAc                                                                              >  1:3670509/1‑150 (MQ=255)

                                                                   aGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCAc                                                                              <  2:57099/150‑1 (MQ=255)

                                                                     ccAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTa                                                                            <  1:3253507/150‑1 (MQ=255)

                                                                         aCTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCg                                                                        >  2:2532154/1‑150 (MQ=255)

                                                                          cTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCgg                                                                       <  2:1692847/150‑1 (MQ=255)

                                                                          cTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCgg                                                                       <  2:3587269/150‑1 (MQ=255)

                                                                              ccAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAg                                                                   <  1:2379236/150‑1 (MQ=255)

                                                                               caacaaCGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAgg                                                                  <  2:3550085/150‑1 (MQ=255)

                                                                               caacaaCGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAgg                                                                  <  1:1770942/150‑1 (MQ=255)

                                                                               caacaaCGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAgg                                                                  <  1:1757855/150‑1 (MQ=255)

                                                                                aacaacGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACAGAGCGAGCGTTAACCCCACTAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAggg                                                                 <  2:3101911/150‑1 (MQ=255)

                                                                                 acaacGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGt                                                                >  1:2896883/1‑150 (MQ=255)

                                                                                  caacGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGtt                                                               <  1:2019140/150‑1 (MQ=255)

                                                                                   aacGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTg                                                              <  2:2309554/150‑1 (MQ=255)

                                                                                    acGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTgc                                                             <  2:642080/150‑1 (MQ=255)

                                                                                      gggCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTgcgc                                                           >  1:873621/1‑150 (MQ=255)

                                                                                         cGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCAGAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTtc                                                        <  1:3616892/150‑1 (MQ=255)

                                                                                           tGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTtcat                                                      <  1:974486/150‑1 (MQ=255)

                                                                                            gCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTtcatc                                                     <  2:2622901/150‑1 (MQ=255)

                                                                                               gTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGc                                                  >  2:2551938/1‑150 (MQ=255)

                                                                                                tCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGcc                                                 <  1:522934/150‑1 (MQ=255)

                                                                                                   ggTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATa                                              >  1:1492357/1‑150 (MQ=255)

                                                                                                    gTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATaa                                             <  1:1685043/150‑1 (MQ=255)

                                                                                                       tGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATcc                                          >  2:3205863/1‑150 (MQ=255)

                                                                                                        gCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCg                                         <  2:3155784/150‑1 (MQ=255)

                                                                                                        gCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCg                                         <  1:2596247/150‑1 (MQ=255)

                                                                                                          cAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGc                                       >  2:221034/1‑150 (MQ=255)

                                                                                                          cAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGc                                       >  2:2465434/1‑150 (MQ=255)

                                                                                                           aGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCg                                      <  1:118936/150‑1 (MQ=255)

                                                                                                               cAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCATGCGTTACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTAGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAAt                                   <  1:1146254/150‑1 (MQ=255)

                                                                                                                 gATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCACGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGAt                                >  2:3132635/1‑150 (MQ=255)

                                                                                                                  aTCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATc                               >  1:3457242/1‑150 (MQ=255)

                                                                                                                   tCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATcc                              <  1:637406/150‑1 (MQ=255)

                                                                                                                      cAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAcg                           >  1:3151359/1‑150 (MQ=255)

                                                                                                                      cAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAcg                           >  1:3624851/1‑150 (MQ=255)

                                                                                                                      cAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAcg                           >  2:1739788/1‑150 (MQ=255)

                                                                                                                        aTGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAcgcc                         <  1:817710/150‑1 (MQ=255)

                                                                                                                         tGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAcgccg                        >  1:273989/1‑150 (MQ=255)

                                                                                                                                gcgagcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcga                 >  2:2858713/1‑150 (MQ=255)

                                                                                                                                   agcgTTAACCCCTCTAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgacga              <  2:3396047/150‑1 (MQ=255)

                                                                                                                                   agcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgacga              >  2:37694/1‑150 (MQ=255)

                                                                                                                                     cgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACa            >  2:1046074/1‑150 (MQ=255)

                                                                                                                                      gTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACAt           >  2:3788474/1‑150 (MQ=255)

                                                                                                                                       ttAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACAtt          >  2:373500/1‑150 (MQ=255)

                                                                                                                                       ttAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACAtt          >  1:1496726/1‑150 (MQ=255)

                                                                                                                                        tAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTa         >  1:1532342/1‑150 (MQ=255)

                                                                                                                                          aCCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTaaa       >  1:3062182/1‑150 (MQ=255)

                                                                                                                                           ccccAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAg      >  1:545506/1‑150 (MQ=255)

                                                                                                                                            cccAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAgg     <  2:2791882/150‑1 (MQ=255)

                                                                                                                                            cccAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAgg     >  2:1851252/1‑150 (MQ=255)

                                                                                                                                             ccAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGc    <  2:334097/150‑1 (MQ=255)

                                                                                                                                              cAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGcc   >  1:2603260/1‑150 (MQ=255)

                                                                                                                                               aaTAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCg  <  2:2597845/150‑1 (MQ=255)

                                                                                                                                               aaTAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCg  >  1:1473980/1‑150 (MQ=255)

GGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCG  >  CP009273/2638970‑2639262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTT  >  CP009273/2638967‑2639264

TCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATA                                                                                                                                                       <  SRR19973061.2597106/150‑1 (MQ=60)

    GCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCC                                                                                                                                                   <  SRR19973061.328737/150‑1 (MQ=60)

    GCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCC                                                                                                                                                   <  SRR19973061.165470/150‑1 (MQ=60)

       GATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGG                                                                                                                                                <  SRR19973061.1304317/150‑1 (MQ=60)

        ATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGC                                                                                                                                               >  SRR19973061.2909102/1‑150 (MQ=60)

           CGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTC                                                                                                                                            <  SRR19973061.969633/150‑1 (MQ=60)

            GCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCA                                                                                                                                           <  SRR19973061.1376473/150‑1 (MQ=60)

              ACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACG                                                                                                                                         >  SRR19973061.2622189/1‑150 (MQ=60)

                TGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGAT                                                                                                                                       >  SRR19973061.3292735/1‑150 (MQ=60)

                    CGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCC                                                                                                                                   >  SRR19973061.3559172/1‑150 (MQ=60)

                    CGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCC                                                                                                                                   >  SRR19973061.1660376/1‑150 (MQ=60)

                    CGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGAGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATATCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCC                                                                                                                                   >  SRR19973061.1488663/1‑150 (MQ=60)

                     GACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCA                                                                                                                                  >  SRR19973061.3193247/1‑150 (MQ=60)

                       CAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATA                                                                                                                                <  SRR19973061.1078650/150‑1 (MQ=60)

                       CAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATA                                                                                                                                >  SRR19973061.3189738/1‑150 (MQ=60)

                        AGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAG                                                                                                                               <  SRR19973061.894318/150‑1 (MQ=60)

                          TAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCT                                                                                                                             >  SRR19973061.3186714/1‑150 (MQ=60)

                          TAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCT                                                                                                                             <  SRR19973061.3341365/150‑1 (MQ=60)

                            AGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGG                                                                                                                           >  SRR19973061.3301202/1‑150 (MQ=60)

                            AGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGG                                                                                                                           >  SRR19973061.2289798/1‑150 (MQ=60)

                             GATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGT                                                                                                                          <  SRR19973061.2090327/150‑1 (MQ=60)

                                    TTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTT                                                                                                                   <  SRR19973061.3165083/150‑1 (MQ=60)

                                    TTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTT                                                                                                                   >  SRR19973061.2604514/1‑150 (MQ=60)

                                    TTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTT                                                                                                                   >  SRR19973061.693888/1‑150 (MQ=60)

                                     TGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTT                                                                                                                  >  SRR19973061.1685334/1‑150 (MQ=60)

                                     TGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTT                                                                                                                  >  SRR19973061.1685341/1‑150 (MQ=60)

                                     TGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTT                                                                                                                  >  SRR19973061.785307/1‑150 (MQ=60)

                                     TGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTT                                                                                                                  >  SRR19973061.2677670/1‑150 (MQ=60)

                                     TGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTT                                                                                                                  <  SRR19973061.124307/150‑1 (MQ=60)

                                      GACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTT                                                                                                                 <  SRR19973061.839609/150‑1 (MQ=60)

                                      GACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAAAGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTT                                                                                                                 >  SRR19973061.3701974/1‑150 (MQ=60)

                                        CCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCC                                                                                                               <  SRR19973061.14016/150‑1 (MQ=60)

                                        CCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCC                                                                                                               <  SRR19973061.1930323/150‑1 (MQ=60)

                                        CCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCC                                                                                                               <  SRR19973061.3225029/150‑1 (MQ=60)

                                         CTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCA                                                                                                              >  SRR19973061.2364208/1‑150 (MQ=60)

                                         CTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCA                                                                                                              >  SRR19973061.3267345/1‑150 (MQ=60)

                                           GATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATG                                                                                                            >  SRR19973061.1926785/1‑150 (MQ=60)

                                               CAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAG                                                                                                        >  SRR19973061.3505688/1‑150 (MQ=60)

                                               CAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAG                                                                                                        >  SRR19973061.3505790/1‑150 (MQ=60)

                                                AACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT                                                                                                       >  SRR19973061.3576169/1‑150 (MQ=60)

                                                AACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT                                                                                                       >  SRR19973061.2630981/1‑150 (MQ=60)

                                                AACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT                                                                                                       >  SRR19973061.2629640/1‑150 (MQ=60)

                                                AACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT                                                                                                       >  SRR19973061.2767704/1‑150 (MQ=60)

                                                AACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT                                                                                                       >  SRR19973061.561130/1‑150 (MQ=60)

                                                   AATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGG                                                                                                   >  SRR19973061.1918258/1‑150 (MQ=60)

                                                      GGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCG                                                                                                <  SRR19973061.289063/150‑1 (MQ=60)

                                                      GGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCG                                                                                                <  SRR19973061.289218/150‑1 (MQ=60)

                                                        TACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGAC                                                                                              >  SRR19973061.3163895/1‑150 (MQ=60)

                                                         ACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACG                                                                                             >  SRR19973061.340267/1‑150 (MQ=60)

                                                           GGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCG                                                                                           <  SRR19973061.3780326/150‑1 (MQ=60)

                                                             CACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCG                                                                                         >  SRR19973061.47018/1‑150 (MQ=60)

                                                              ACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGG                                                                                        <  SRR19973061.1640650/150‑1 (MQ=60)

                                                                TGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCA                                                                                      >  SRR19973061.3211562/1‑150 (MQ=60)

                                                                     AAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCA                                                                                 <  SRR19973061.2530625/150‑1 (MQ=60)

                                                                     AAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCA                                                                                 <  SRR19973061.2530327/150‑1 (MQ=60)

                                                                     AAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCA                                                                                 <  SRR19973061.2530644/150‑1 (MQ=60)

                                                                      AGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCAC                                                                                >  SRR19973061.3679893/1‑150 (MQ=60)

                                                                        CCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTA                                                                              <  SRR19973061.3260640/150‑1 (MQ=60)

                                                                        CCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTA                                                                              <  SRR19973061.3261877/150‑1 (MQ=60)

                                                                          AAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATC                                                                            >  SRR19973061.3146562/1‑150 (MQ=60)

                                                                          AAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATC                                                                            >  SRR19973061.2008500/1‑150 (MQ=60)

                                                                           AACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCC                                                                           >  SRR19973061.537657/1‑150 (MQ=60)

                                                                            ACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCG                                                                          <  SRR19973061.1694056/150‑1 (MQ=60)

                                                                                 CCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAG                                                                     <  SRR19973061.473695/150‑1 (MQ=60)

                                                                                 CCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAG                                                                     <  SRR19973061.2385293/150‑1 (MQ=60)

                                                                                  CAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGG                                                                    <  SRR19973061.1775376/150‑1 (MQ=60)

                                                                                  CAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGG                                                                    <  SRR19973061.1762258/150‑1 (MQ=60)

                                                                                    ACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGT                                                                  >  SRR19973061.2904317/1‑150 (MQ=60)

                                                                                     CAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTCTCCGGCAAGGGTT                                                                 >  SRR19973061.3039442/1‑150 (MQ=60)

                                                                                     CAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTT                                                                 <  SRR19973061.2024214/150‑1 (MQ=60)

                                                                                     CAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTT                                                                 <  SRR19973061.1576741/150‑1 (MQ=60)

                                                                                      AACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTG                                                                <  SRR19973061.2103782/150‑1 (MQ=60)

                                                                                         GGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGC                                                             >  SRR19973061.875889/1‑150 (MQ=60)

                                                                                          GGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCT                                                            <  SRR19973061.2080386/150‑1 (MQ=60)

                                                                                           GCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTT                                                           <  SRR19973061.2513414/150‑1 (MQ=60)

                                                                                            CGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTC                                                          <  SRR19973061.3625501/150‑1 (MQ=60)

                                                                                            CGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTC                                                          <  SRR19973061.3626251/150‑1 (MQ=60)

                                                                                            CGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCAGAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTC                                                          <  SRR19973061.3626143/150‑1 (MQ=60)

                                                                                             GTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCA                                                         >  SRR19973061.3641935/1‑150 (MQ=60)

                                                                                             GTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCA                                                         >  SRR19973061.2378551/1‑150 (MQ=60)

                                                                                              TGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCAT                                                        <  SRR19973061.976967/150‑1 (MQ=60)

                                                                                                   TCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCC                                                   <  SRR19973061.524313/150‑1 (MQ=60)

                                                                                                      GGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATA                                                >  SRR19973061.1496101/1‑150 (MQ=60)

                                                                                                      GGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATA                                                >  SRR19973061.1495776/1‑150 (MQ=60)

                                                                                                       GTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAA                                               <  SRR19973061.1689280/150‑1 (MQ=60)

                                                                                                       GTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAA                                               >  SRR19973061.3489237/1‑150 (MQ=60)

                                                                                                           GCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCG                                           >  SRR19973061.3695324/1‑150 (MQ=60)

                                                                                                           GCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCG                                           <  SRR19973061.2602909/150‑1 (MQ=60)

                                                                                                             CAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGC                                         >  SRR19973061.813945/1‑150 (MQ=60)

                                                                                                              AGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCG                                        <  SRR19973061.2898295/150‑1 (MQ=60)

                                                                                                              AGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCG                                        <  SRR19973061.119330/150‑1 (MQ=60)

                                                                                                                 CCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAAT                                     <  SRR19973061.1149207/150‑1 (MQ=60)

                                                                                                                  CAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCATGCGTTACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTAGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAAT                                     <  SRR19973061.1149170/150‑1 (MQ=60)

                                                                                                                     ATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATC                                 >  SRR19973061.3466107/1‑150 (MQ=60)

                                                                                                                     ATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATC                                 >  SRR19973061.662383/1‑150 (MQ=60)

                                                                                                                      TCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCC                                <  SRR19973061.639024/150‑1 (MQ=60)

                                                                                                                      TCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCC                                <  SRR19973061.1132514/150‑1 (MQ=60)

                                                                                                                         CAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACG                             >  SRR19973061.3159461/1‑150 (MQ=60)

                                                                                                                         CAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACG                             >  SRR19973061.3634122/1‑150 (MQ=60)

                                                                                                                         CAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACG                             >  SRR19973061.3160202/1‑150 (MQ=60)

                                                                                                                           ATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCC                           <  SRR19973061.819848/150‑1 (MQ=60)

                                                                                                                            TGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG                          >  SRR19973061.274739/1‑150 (MQ=60)

                                                                                                                            TGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG                          >  SRR19973061.274740/1‑150 (MQ=60)

                                                                                                                            TGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG                          <  SRR19973061.1954987/150‑1 (MQ=60)

                                                                                                                            TGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG                          >  SRR19973061.274792/1‑150 (MQ=60)

                                                                                                                            TGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG                          >  SRR19973061.1805140/1‑150 (MQ=60)

                                                                                                                               CATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCC                       >  SRR19973061.741634/1‑150 (MQ=60)

                                                                                                                               CATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCC                       >  SRR19973061.741608/1‑150 (MQ=60)

                                                                                                                               CATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCC                       >  SRR19973061.1533477/1‑150 (MQ=60)

                                                                                                                                ATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCC                      <  SRR19973061.605167/150‑1 (MQ=60)

                                                                                                                                  AGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCG                    >  SRR19973061.401094/1‑150 (MQ=60)

                                                                                                                                      AGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGA                >  SRR19973061.1217145/1‑150 (MQ=60)

                                                                                                                                          TTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATT            >  SRR19973061.1500475/1‑150 (MQ=60)

                                                                                                                                          TTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATT            >  SRR19973061.1499905/1‑150 (MQ=60)

                                                                                                                                          TTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATT            >  SRR19973061.1453167/1‑150 (MQ=60)

                                                                                                                                           TAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTA           >  SRR19973061.1535512/1‑150 (MQ=60)

                                                                                                                                           TAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTA           >  SRR19973061.1536163/1‑150 (MQ=60)

                                                                                                                                             ACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAA         >  SRR19973061.3070093/1‑150 (MQ=60)

                                                                                                                                             ACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAA         <  SRR19973061.2333482/150‑1 (MQ=60)

                                                                                                                                              CCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAG        >  SRR19973061.546944/1‑150 (MQ=60)

                                                                                                                                              CCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAG        >  SRR19973061.1771662/1‑150 (MQ=60)

                                                                                                                                              CCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAG        >  SRR19973061.1771678/1‑150 (MQ=60)

                                                                                                                                                CCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGC      >  SRR19973061.226477/1‑150 (MQ=60)

                                                                                                                                                 CAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCC     >  SRR19973061.2609947/1‑150 (MQ=60)

                                                                                                                                                  AATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCG    >  SRR19973061.1477681/1‑150 (MQ=60)

                                                                                                                                                  AATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCG    >  SRR19973061.3651520/1‑150 (MQ=60)

                                                                                                                                                    TAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTT  <  SRR19973061.3785379/150‑1 (MQ=60)

                                                                                                                                                    TAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTT  <  SRR19973061.1634673/150‑1 (MQ=60)

TCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGT‑GGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTT  >  CP009273/2638967‑2639264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`