Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,639,116 C→A 100% A523A (GCG→GCT)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,639,1160CA99.0% 683.8 / ‑2.0 235A523A (GCG→GCT) pbpCpenicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (146/85);  total (146/89)
Fisher's exact test for biased strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

GGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCC  >  CP009273/2638970‑2639261
                                                                                                                                                  |                                                                                                                                                 
ggCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGcc                                                                                                                                                >  2:3427808/1‑150 (MQ=255)
 gCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATACAACCATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGccc                                                                                                                                               <  2:2254792/150‑1 (MQ=255)
     aTTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGc                                                                                                                                           >  2:78613/1‑150 (MQ=255)
     aTTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGc                                                                                                                                           <  1:3948120/150‑1 (MQ=255)
     aTTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGc                                                                                                                                           >  1:3769129/1‑150 (MQ=255)
        cGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTc                                                                                                                                        >  2:1650983/1‑150 (MQ=255)
          cACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCAc                                                                                                                                      >  2:582284/1‑150 (MQ=255)
          cACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCAc                                                                                                                                      <  2:4478223/150‑1 (MQ=255)
           aCTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACg                                                                                                                                     <  2:3300538/150‑1 (MQ=255)
           aCTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACg                                                                                                                                     <  1:3127110/150‑1 (MQ=255)
             tGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGAt                                                                                                                                   <  1:886526/150‑1 (MQ=255)
                 cgACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGcc                                                                                                                               >  1:5044934/1‑150 (MQ=255)
                  gACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCa                                                                                                                              <  2:1217063/150‑1 (MQ=255)
                  gACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACACCAATAGCCCAGGCGACACGATAGCCa                                                                                                                              >  1:4476456/1‑150 (MQ=255)
                   aCAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCAt                                                                                                                             >  1:2188125/1‑150 (MQ=255)
                     aGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCCGGCGTCACGATAGCCagag                                                                                                                           >  2:3308082/1‑150 (MQ=255)
                     aGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAg                                                                                                                           >  1:4412298/1‑150 (MQ=255)
                      gTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGc                                                                                                                          >  1:1949769/1‑150 (MQ=255)
                      gTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGc                                                                                                                          <  2:1104078/150‑1 (MQ=255)
                      gTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGc                                                                                                                          >  1:95555/1‑150 (MQ=255)
                      gTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGc                                                                                                                          >  1:3616810/1‑150 (MQ=255)
                      gTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGc                                                                                                                          >  1:2577388/1‑150 (MQ=255)
                        aaGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTg                                                                                                                        >  1:2085426/1‑150 (MQ=255)
                         aGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTgg                                                                                                                       >  2:4366030/1‑150 (MQ=255)
                         aGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTgg                                                                                                                       <  1:5213370/150‑1 (MQ=255)
                          gATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGt                                                                                                                      >  2:3961800/1‑150 (MQ=255)
                           atatTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTg                                                                                                                     >  2:4052264/1‑150 (MQ=255)
                             attattGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGcc                                                                                                                   >  1:4636514/1‑150 (MQ=255)
                                attGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGt                                                                                                                >  1:2701857/1‑150 (MQ=255)
                                  tGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGttt                                                                                                              <  2:485530/150‑1 (MQ=255)
                                     ccTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTcc                                                                                                           <  1:3327029/150‑1 (MQ=255)
                                       tGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCAt                                                                                                         <  2:1026791/150‑1 (MQ=255)
                                       tGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCAt                                                                                                         <  2:4390767/150‑1 (MQ=255)
                                          ttCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCCTCGCTGGTGCCCGTTTTCCCTGcc                                                                                                      >  2:1473201/1‑150 (MQ=255)
                                           acaacaaTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCa                                                                                                     >  2:1792439/2‑150 (MQ=255)
                                              acaaTGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTg                                                                                                  <  1:3865117/150‑1 (MQ=255)
                                                  tGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGc                                                                                              >  1:4610566/1‑150 (MQ=255)
                                                    gTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGa                                                                                            >  1:3638182/1‑150 (MQ=255)
                                                      aCGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGAcg                                                                                          <  1:1336831/150‑1 (MQ=255)
                                                          cACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGAcgcgcg                                                                                      <  2:3392830/150‑1 (MQ=255)
                                                           aCTTGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCgg                                                                                     >  1:2519889/1‑150 (MQ=255)
                                                           aCTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCgg                                                                                     <  1:3409582/150‑1 (MQ=255)
                                                             tGGCAAAGCCAAACTGCCCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCa                                                                                   <  2:664621/150‑1 (MQ=255)
                                                              ggCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCaa                                                                                  >  2:683551/1‑150 (MQ=255)
                                                               gCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAg                                                                                 <  1:651987/150‑1 (MQ=255)
                                                                  aaGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCa                                                                              >  1:3066971/1‑150 (MQ=255)
                                                                    gCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAAGAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACt                                                                            <  2:323584/150‑1 (MQ=255)
                                                                     ccAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTa                                                                           <  1:2306169/150‑1 (MQ=255)
                                                                      cAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTAt                                                                          >  1:1119927/1‑150 (MQ=255)
                                                                      cAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTAt                                                                          <  1:2895370/150‑1 (MQ=255)
                                                                       aaaCTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATc                                                                         >  1:3465133/1‑150 (MQ=255)
                                                                         aCTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCg                                                                       <  2:1711692/150‑1 (MQ=255)
                                                                          cTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCgg                                                                      <  1:422413/150‑1 (MQ=255)
                                                                           tGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTCTCCGGc                                                                     >  2:3014127/1‑150 (MQ=255)
                                                                           tGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGc                                                                     >  1:755777/1‑150 (MQ=255)
                                                                                aacaacGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAggg                                                                >  2:4259221/1‑150 (MQ=255)
                                                                                   aacGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTg                                                             <  2:4620379/150‑1 (MQ=255)
                                                                                   aacGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTg                                                             >  1:491829/1‑150 (MQ=255)
                                                                                      gggCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTgcgc                                                          >  1:1100035/1‑150 (MQ=255)
                                                                                      gggCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTgcgc                                                          >  1:1203510/1‑150 (MQ=255)
                                                                                       ggCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCt                                                         >  1:1993492/1‑150 (MQ=255)
                                                                                        gCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCtt                                                        <  2:2019838/150‑1 (MQ=255)
                                                                                         cGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTtc                                                       >  1:3645811/1‑150 (MQ=255)
                                                                                           tGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTtcat                                                     <  1:2861979/150‑1 (MQ=255)
                                                                                            gCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTtcatc                                                    <  1:4851723/150‑1 (MQ=255)
                                                                                             ccGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTtcatca                                                   <  2:2515665/150‑1 (MQ=255)
                                                                                              cGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAg                                                  >  2:2943272/1‑150 (MQ=255)
                                                                                                tCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGcc                                                <  2:4785915/150‑1 (MQ=255)
                                                                                                    gTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATaa                                            <  1:2724647/150‑1 (MQ=255)
                                                                                                     tCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCACGGGTTGCGCTTCATCAGCCATAAt                                           >  2:3762309/1‑150 (MQ=255)
                                                                                                      cTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATc                                          >  1:4751466/1‑150 (MQ=255)
                                                                                                       tGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATcc                                         >  2:2584258/1‑150 (MQ=255)
                                                                                                       tGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATcc                                         >  2:1907543/1‑150 (MQ=255)
                                                                                                         ccAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCgg                                       >  2:90997/1‑150 (MQ=255)
                                                                                                           aGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCg                                     >  1:1676059/1‑150 (MQ=255)
                                                                                                              ccAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAAt                                  >  2:2214332/1‑150 (MQ=255)
                                                                                                               cAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATg                                 <  1:186184/150‑1 (MQ=255)
                                                                                                               cAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATg                                 >  1:2947793/1‑150 (MQ=255)
                                                                                                                aGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGa                                >  2:5021868/1‑150 (MQ=255)
                                                                                                                aGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGa                                >  2:4888037/1‑150 (MQ=255)
                                                                                                                  aTCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCACGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATc                              >  2:51258/1‑150 (MQ=255)
                                                                                                                    cccAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCa                            >  1:683950/1‑150 (MQ=255)
                                                                                                                    cccAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCa                            >  1:2103277/1‑150 (MQ=255)
                                                                                                                     ccAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAc                           >  1:4208301/1‑150 (MQ=255)
                                                                                                                        aTGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAcgcc                        >  1:3915496/1‑150 (MQ=255)
                                                                                                                            cATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccc                    <  2:3616810/150‑1 (MQ=255)
                                                                                                                             aTAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGcccc                   >  1:2784689/1‑150 (MQ=255)
                                                                                                                                 cgagcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgac               <  2:3638182/150‑1 (MQ=255)
                                                                                                                                 cgagcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgac               <  1:4398864/150‑1 (MQ=255)
                                                                                                                                 cgagcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgac               >  2:2041038/1‑150 (MQ=255)
                                                                                                                                  gagcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgacg              <  2:4615927/150‑1 (MQ=255)
                                                                                                                                  gagcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgacg              >  2:5181667/1‑150 (MQ=255)
                                                                                                                                  gagcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgacg              >  2:2001257/1‑150 (MQ=255)
                                                                                                                                   agcgTTAACCCCAATAGCCCAGGCGTCACGAAAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCcgacga             >  2:4701879/1‑150 (MQ=255)
                                                                                                                                    gcgTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCgacgac            <  2:209071/150‑1 (MQ=255)
                                                                                                                                     cgTTAACCCCAATAGCCCAGGCGTCACGAGAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACa           <  2:4086005/150‑1 (MQ=255)
                                                                                                                                      tttAACCCCACTAGCCCAGGCGTCTCGATAGCCATAGCTGGTGACAGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGTCTAGGGTTGCGCTTCATCAGCCATAATCCGGCGACTGATCCACGCCGCCCCCGTCGTCAt          <  2:138268/149‑1 (MQ=255)
                                                                                                                                      gTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACAt          <  2:1365581/150‑1 (MQ=255)
                                                                                                                                        tAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTa        <  2:2566350/150‑1 (MQ=255)
                                                                                                                                            cccAATAGCCCATGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCAATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAgg    >  1:101054/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCC  >  CP009273/2638970‑2639261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTC  >  CP009273/2638967‑2639265
                                                                                                                                                     |                                                                                                                                                     
TCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATA                                                                                                                                                       <  SRR19973062.3345132/150‑1 (MQ=60)
TCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATA                                                                                                                                                       <  SRR19973062.4102210/150‑1 (MQ=60)
        ATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGC                                                                                                                                               >  SRR19973062.3788440/1‑150 (MQ=60)
        ATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGC                                                                                                                                               <  SRR19973062.3968164/150‑1 (MQ=60)
              ACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCTATAGCCCAGGCGTCACG                                                                                                                                         <  SRR19973062.3143399/150‑1 (MQ=60)
              ACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACG                                                                                                                                         <  SRR19973062.3143204/150‑1 (MQ=60)
                TGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGAT                                                                                                                                       <  SRR19973062.890321/150‑1 (MQ=60)
                 GCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATA                                                                                                                                      <  SRR19973062.2483963/150‑1 (MQ=60)
                  CGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAG                                                                                                                                     >  SRR19973062.481285/1‑150 (MQ=60)
                    CGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCC                                                                                                                                   >  SRR19973062.5071682/1‑150 (MQ=60)
                     GACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCA                                                                                                                                  >  SRR19973062.3752071/1‑150 (MQ=60)
                     GACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCA                                                                                                                                  >  SRR19973062.4499425/1‑150 (MQ=60)
                     GACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCA                                                                                                                                  <  SRR19973062.29623/150‑1 (MQ=60)
                     GACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCA                                                                                                                                  <  SRR19973062.29620/150‑1 (MQ=60)
                     GACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACACCAATAGCCCAGGCGACACGATAGCCA                                                                                                                                  >  SRR19973062.4499413/1‑150 (MQ=60)
                      ACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCAT                                                                                                                                 >  SRR19973062.2199124/1‑150 (MQ=60)
                      ACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCAT                                                                                                                                 >  SRR19973062.2199121/1‑150 (MQ=60)
                      ACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCAT                                                                                                                                 <  SRR19973062.4877342/150‑1 (MQ=60)
                        AGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAG                                                                                                                               >  SRR19973062.4434855/1‑150 (MQ=60)
                        AGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAG                                                                                                                               >  SRR19973062.4434644/1‑150 (MQ=60)
                         TTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGC                                                                                                                              <  SRR19973062.2828857/150‑1 (MQ=60)
                         GTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGC                                                                                                                              >  SRR19973062.1959779/1‑150 (MQ=60)
                         GTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGC                                                                                                                              >  SRR19973062.2591076/1‑150 (MQ=60)
                         GTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGC                                                                                                                              >  SRR19973062.3635257/1‑150 (MQ=60)
                         GTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGC                                                                                                                              >  SRR19973062.3635504/1‑150 (MQ=60)
                         GTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGC                                                                                                                              >  SRR19973062.5248084/1‑150 (MQ=60)
                         GTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGC                                                                                                                              >  SRR19973062.96178/1‑150 (MQ=60)
                         GTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGC                                                                                                                              >  SRR19973062.4246121/1‑150 (MQ=60)
                          TAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCT                                                                                                                             <  SRR19973062.714510/150‑1 (MQ=60)
                           AAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTG                                                                                                                            >  SRR19973062.2095988/1‑150 (MQ=60)
                           AAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTG                                                                                                                            >  SRR19973062.2096097/1‑150 (MQ=60)
                           AAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTG                                                                                                                            >  SRR19973062.2096090/1‑150 (MQ=60)
                            AGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGG                                                                                                                           <  SRR19973062.5240866/150‑1 (MQ=60)
                                ATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCC                                                                                                                       >  SRR19973062.4660771/1‑150 (MQ=60)
                                   ATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGT                                                                                                                    >  SRR19973062.2716031/1‑150 (MQ=60)
                                   ATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGT                                                                                                                    >  SRR19973062.2879746/1‑150 (MQ=60)
                                      GACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTT                                                                                                                 <  SRR19973062.3841273/150‑1 (MQ=60)
                                        CCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCC                                                                                                               <  SRR19973062.3344209/150‑1 (MQ=60)
                                           GATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATG                                                                                                            >  SRR19973062.410239/1‑150 (MQ=60)
                                              TCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCA                                                                                                         <  SRR19973062.3768282/150‑1 (MQ=60)
                                              TCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCA                                                                                                         >  SRR19973062.495931/1‑150 (MQ=60)
                                               CAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAG                                                                                                        >  SRR19973062.2128802/1‑150 (MQ=60)
                                                 ACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTG                                                                                                      >  SRR19973062.3187067/1‑150 (MQ=60)
                                                 ACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTG                                                                                                      <  SRR19973062.3884846/150‑1 (MQ=60)
                                                 ACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTG                                                                                                      <  SRR19973062.3884876/150‑1 (MQ=60)
                                                 ACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTG                                                                                                      <  SRR19973062.2634417/150‑1 (MQ=60)
                                                 ACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTG                                                                                                      <  SRR19973062.1494355/150‑1 (MQ=60)
                                                  CAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGG                                                                                                     >  SRR19973062.4581065/1‑150 (MQ=60)
                                                     TGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGC                                                                                                  >  SRR19973062.4634692/1‑150 (MQ=60)
                                                      GGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCG                                                                                                 <  SRR19973062.938318/150‑1 (MQ=60)
                                                       GTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGA                                                                                                >  SRR19973062.3656801/1‑150 (MQ=60)
                                                         ACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACG                                                                                              <  SRR19973062.1342889/150‑1 (MQ=60)
                                                           GGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCG                                                                                            >  SRR19973062.5015037/1‑150 (MQ=60)
                                                            GCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGC                                                                                           >  SRR19973062.3501989/1‑150 (MQ=60)
                                                              ACTTGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGG                                                                                         >  SRR19973062.2533357/1‑150 (MQ=60)
                                                              ACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGG                                                                                         <  SRR19973062.3427047/150‑1 (MQ=60)
                                                               CTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAAGGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGC                                                                                        >  SRR19973062.4640069/1‑150 (MQ=60)
                                                                TGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCA                                                                                       >  SRR19973062.4666967/1‑150 (MQ=60)
                                                                TGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCA                                                                                       >  SRR19973062.1997592/1‑150 (MQ=60)
                                                                TGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCA                                                                                       >  SRR19973062.910187/1‑150 (MQ=60)
                                                                  GCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAG                                                                                     <  SRR19973062.655032/150‑1 (MQ=60)
                                                                  GCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAG                                                                                     >  SRR19973062.4450051/1‑150 (MQ=60)
                                                                     AAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCA                                                                                  >  SRR19973062.3082869/1‑150 (MQ=60)
                                                                      AGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCAC                                                                                 <  SRR19973062.1882119/150‑1 (MQ=60)
                                                                        CCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCACTAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTA                                                                               <  SRR19973062.2053552/150‑1 (MQ=60)
                                                                        CCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTA                                                                               <  SRR19973062.2318250/150‑1 (MQ=60)
                                                                         CAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTAT                                                                              >  SRR19973062.1125179/1‑150 (MQ=60)
                                                                         CAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTAT                                                                              <  SRR19973062.2910653/150‑1 (MQ=60)
                                                                          AAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATC                                                                             >  SRR19973062.3482781/1‑150 (MQ=60)
                                                                             CTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGG                                                                          <  SRR19973062.424230/150‑1 (MQ=60)
                                                                             CTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGG                                                                          <  SRR19973062.424118/150‑1 (MQ=60)
                                                                              TGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGC                                                                         >  SRR19973062.759192/1‑150 (MQ=60)
                                                                                      AACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTG                                                                 >  SRR19973062.494105/1‑150 (MQ=60)
                                                                                       ACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGC                                                                >  SRR19973062.2568088/1‑150 (MQ=60)
                                                                                       ACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGC                                                                >  SRR19973062.3041201/1‑150 (MQ=60)
                                                                                         GGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCTAGGCACTATCCGGCAAGGGTTGCGC                                                              >  SRR19973062.1105239/1‑150 (MQ=60)
                                                                                         GGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGC                                                              >  SRR19973062.1209174/1‑150 (MQ=60)
                                                                                         GGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGC                                                              >  SRR19973062.1105211/1‑150 (MQ=60)
                                                                                         GGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGC                                                              >  SRR19973062.1209124/1‑150 (MQ=60)
                                                                                          GGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCT                                                             >  SRR19973062.2003748/1‑150 (MQ=60)
                                                                                            CGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTC                                                           >  SRR19973062.3664473/1‑150 (MQ=60)
                                                                                            CGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTC                                                           >  SRR19973062.3664477/1‑150 (MQ=60)
                                                                                              TGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCAT                                                         <  SRR19973062.2876998/150‑1 (MQ=60)
                                                                                              TGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCAT                                                         <  SRR19973062.2877107/150‑1 (MQ=60)
                                                                                               GCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATC                                                        <  SRR19973062.4876766/150‑1 (MQ=60)
                                                                                                 CGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAG                                                      <  SRR19973062.2786693/150‑1 (MQ=60)
                                                                                                       GTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAA                                                <  SRR19973062.2738979/150‑1 (MQ=60)
                                                                                                         CTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATC                                              >  SRR19973062.4776198/1‑150 (MQ=60)
                                                                                                         CTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATC                                              >  SRR19973062.1144821/1‑150 (MQ=60)
                                                                                                           GCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCG                                            <  SRR19973062.3136070/150‑1 (MQ=60)
                                                                                                            CCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGG                                           <  SRR19973062.2652126/150‑1 (MQ=60)
                                                                                                              AGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCG                                         >  SRR19973062.674267/1‑150 (MQ=60)
                                                                                                              AGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCG                                         >  SRR19973062.674316/1‑150 (MQ=60)
                                                                                                              AGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCG                                         >  SRR19973062.1684293/1‑150 (MQ=60)
                                                                                                                 CCAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAAT                                      >  SRR19973062.3384975/1‑150 (MQ=60)
                                                                                                                  CAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATG                                     >  SRR19973062.2963289/1‑150 (MQ=60)
                                                                                                                  CAGATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATG                                     <  SRR19973062.187199/150‑1 (MQ=60)
                                                                                                                    GATCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGAT                                   >  SRR19973062.5153605/1‑150 (MQ=60)
                                                                                                                      TCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCC                                 <  SRR19973062.46936/150‑1 (MQ=60)
                                                                                                                      TCCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCC                                 >  SRR19973062.2310549/1‑150 (MQ=60)
                                                                                                                       CCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCA                                >  SRR19973062.687120/1‑150 (MQ=60)
                                                                                                                       CCCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCA                                >  SRR19973062.2114008/1‑150 (MQ=60)
                                                                                                                        CCAATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCAC                               >  SRR19973062.4230122/1‑150 (MQ=60)
                                                                                                                           ATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCC                            <  SRR19973062.105759/150‑1 (MQ=60)
                                                                                                                           ATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCC                            >  SRR19973062.4362325/1‑150 (MQ=60)
                                                                                                                           ATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCC                            >  SRR19973062.100037/1‑150 (MQ=60)
                                                                                                                           ATGACATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCC                            >  SRR19973062.3935425/1‑150 (MQ=60)
                                                                                                                                ATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCCTGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCC                       <  SRR19973062.3936546/150‑1 (MQ=60)
                                                                                                                                ATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCC                       >  SRR19973062.2799401/1‑150 (MQ=60)
                                                                                                                                ATAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCC                       <  SRR19973062.3936545/150‑1 (MQ=60)
                                                                                                                                ATAGCGAGCGTTAACCCCAAGCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCC                       <  SRR19973062.3445335/150‑1 (MQ=60)
                                                                                                                                 TAGCGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCC                      <  SRR19973062.2718816/150‑1 (MQ=60)
                                                                                                                                    CGAGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGAC                   <  SRR19973062.4421382/150‑1 (MQ=60)
                                                                                                                                      AGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGA                 >  SRR19973062.1824590/1‑150 (MQ=60)
                                                                                                                                      AGCGTTAACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGA                 >  SRR19973062.1778086/1‑150 (MQ=60)
                                                                                                                                             ACCCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAA          <  SRR19973062.1174654/150‑1 (MQ=60)
                                                                                                                                               CCCAATAGCCCATGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCAATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGG        >  SRR19973062.101709/1‑150 (MQ=60)
                                                                                                                                               CCCAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGG        <  SRR19973062.3511942/150‑1 (MQ=60)
                                                                                                                                                 CAATAGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCC      >  SRR19973062.834765/1‑150 (MQ=60)
                                                                                                                                                     AGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTC  <  SRR19973062.1161791/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TCCGGCAGATTCGCACTGCGCGACAGTAAGATATTATTGACCTGATTCAACAATGGTACGGCACTGGCAAAGCCAAACTGACCAACAACGGGCGTGCCGTCCGGTCTGCCAGTCCAGATCCCAATGACATAGCGAGCGTTAACCCCAATCGCCCAGGCGTCACGATAGCCATAGCTGGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTC  >  CP009273/2638967‑2639265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑