Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,691,904 T→A A2A (GCA→GCT)  pgpC ← phosphatidylglycerophosphatase C, membrane bound

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,691,9040TA99.6% 760.6 / NA 230A2A (GCA→GCT) pgpCphosphatidylglycerophosphatase C, membrane bound
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (119/110);  minor base C (1/0);  total (120/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

CGCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGA  >  CP009273/2691757‑2692050
                                                                                                                                                   |                                                                                                                                                  
cGCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGc                                                                                                                                                  <  2:1441624/150‑1 (MQ=255)
 gCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGcc                                                                                                                                                 >  2:830079/1‑150 (MQ=255)
 gCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGcc                                                                                                                                                 >  2:3067476/1‑150 (MQ=255)
  cAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCa                                                                                                                                                <  2:3157139/150‑1 (MQ=255)
  cAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCa                                                                                                                                                <  1:1705833/150‑1 (MQ=255)
   aaTGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTCAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCaa                                                                                                                                               >  2:2549377/1‑150 (MQ=255)
   aaTGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCaa                                                                                                                                               >  1:1726337/1‑150 (MQ=255)
      ggCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAAcc                                                                                                                                            <  2:2087344/150‑1 (MQ=255)
      ggCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAAcc                                                                                                                                            <  2:1825468/150‑1 (MQ=255)
          ataATCGGTAACAACGGCAGGACAAGTAACGCATTCAGTGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATAc                                                                                                                                        >  2:225013/1‑150 (MQ=255)
           taATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATAct                                                                                                                                       <  2:2231982/150‑1 (MQ=255)
            aaTCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATActc                                                                                                                                      >  1:1164986/1‑150 (MQ=255)
             aTCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATActcc                                                                                                                                     >  1:704873/1‑150 (MQ=255)
             aTCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATActcc                                                                                                                                     <  1:1173582/150‑1 (MQ=255)
              tCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATActcct                                                                                                                                    <  1:2592744/150‑1 (MQ=255)
                 gTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTg                                                                                                                                 >  2:2246886/1‑150 (MQ=255)
                  tAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTgg                                                                                                                                >  1:2287055/1‑150 (MQ=255)
                  tAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTgg                                                                                                                                >  2:2475010/1‑150 (MQ=255)
                     caacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACt                                                                                                                             >  1:3615223/1‑150 (MQ=255)
                      aacGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTg                                                                                                                            >  2:2526316/1‑150 (MQ=255)
                        cGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGaa                                                                                                                          >  1:423855/1‑150 (MQ=255)
                        cGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGaa                                                                                                                          <  2:413250/150‑1 (MQ=255)
                         ggCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGaaa                                                                                                                         >  2:1118394/1‑150 (MQ=255)
                         ggCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGaaa                                                                                                                         >  2:730249/1‑150 (MQ=255)
                          gCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAAc                                                                                                                        >  2:3021178/1‑150 (MQ=255)
                                cAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTACCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTg                                                                                                                  >  2:1008200/1‑150 (MQ=255)
                                 aaGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGa                                                                                                                 <  1:765178/150‑1 (MQ=255)
                                   gTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGAtt                                                                                                               <  2:1081850/150‑1 (MQ=255)
                                     aaCGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTaa                                                                                                             >  1:1057488/1‑150 (MQ=255)
                                      aCGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAAt                                                                                                            <  1:2704599/150‑1 (MQ=255)
                                       cGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAAtt                                                                                                           <  2:2450604/150‑1 (MQ=255)
                                          aTTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTc                                                                                                        >  1:3142422/1‑150 (MQ=255)
                                          aTTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTc                                                                                                        <  1:2438706/150‑1 (MQ=255)
                                          aTTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTc                                                                                                        <  1:3263663/150‑1 (MQ=255)
                                             cAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGct                                                                                                     >  2:3578329/1‑150 (MQ=255)
                                              aGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGcta                                                                                                    <  1:1557285/150‑1 (MQ=255)
                                               gCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGctac                                                                                                   <  2:1175447/150‑1 (MQ=255)
                                                cGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGctact                                                                                                  <  2:3091631/150‑1 (MQ=255)
                                                   ttGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACa                                                                                               >  2:575926/1‑150 (MQ=255)
                                                    tGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACAt                                                                                              >  2:1062496/1‑150 (MQ=255)
                                                     ggCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATa                                                                                             <  1:1852937/150‑1 (MQ=255)
                                                       cGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGc                                                                                           <  1:1977136/150‑1 (MQ=255)
                                                       cGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGc                                                                                           <  1:512417/150‑1 (MQ=255)
                                                        gACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGcc                                                                                          >  2:3626953/1‑150 (MQ=255)
                                                           gtagtaAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTaa                                                                                       >  2:636763/1‑150 (MQ=255)
                                                               taAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACaga                                                                                   <  2:1696851/150‑1 (MQ=255)
                                                                  aTAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACagatag                                                                                <  1:2179704/150‑1 (MQ=255)
                                                                    aGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACagatagat                                                                              <  1:1133381/150‑1 (MQ=255)
                                                                       gcAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGatcat                                                                           <  1:384043/150‑1 (MQ=255)
                                                                        cAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGatcatc                                                                          >  2:3196900/1‑150 (MQ=255)
                                                                         aGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGAtcatca                                                                         >  2:1941554/1‑150 (MQ=255)
                                                                           aaaaCTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACt                                                                       >  1:2233368/1‑150 (MQ=255)
                                                                            aaaCTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACtt                                                                      >  2:1361219/1‑150 (MQ=255)
                                                                             aaCTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACttt                                                                     >  2:2331553/1‑150 (MQ=255)
                                                                              aCTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACtttt                                                                    >  2:3300775/1‑150 (MQ=255)
                                                                               cTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTc                                                                   <  2:2649191/150‑1 (MQ=255)
                                                                                   cGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGc                                                               >  2:1987387/1‑150 (MQ=255)
                                                                                    gAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGca                                                              >  2:2377311/1‑150 (MQ=255)
                                                                                     aaCATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGcaa                                                             >  1:1660442/1‑150 (MQ=255)
                                                                                        atatCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGcaacaa                                                          <  1:830079/150‑1 (MQ=255)
                                                                                        atatCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGcaacaa                                                          >  1:137781/1‑150 (MQ=255)
                                                                                         tatCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAAt                                                         >  1:2511342/1‑150 (MQ=255)
                                                                                          atCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATc                                                        <  2:2765194/150‑1 (MQ=255)
                                                                                           tCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCg                                                       <  1:880824/150‑1 (MQ=255)
                                                                                            ccTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGa                                                      >  1:1592694/1‑150 (MQ=255)
                                                                                            ccTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGAAGCACGTGAGAAGCCAAACCATACTCCTCTGGACTGAAACATCCTGAATAATTTACGCTACTACATAACCAAACAGATAGATCATCACTTTTCCGGCAACAATCGa                                                      >  1:1238939/1‑150 (MQ=255)
                                                                                             ctgctgATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGAc                                                     <  2:401502/150‑1 (MQ=255)
                                                                                             ctgctgATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGAc                                                     >  1:3240838/1‑150 (MQ=255)
                                                                                              tgctgATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGAcc                                                    <  1:3018002/150‑1 (MQ=255)
                                                                                                 tgATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGca                                                 >  1:3061550/1‑150 (MQ=255)
                                                                                                  gATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGcat                                                >  1:145006/1‑150 (MQ=255)
                                                                                                  gATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGcat                                                >  2:1138451/1‑150 (MQ=255)
                                                                                                     gCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGcatcat                                             <  1:2842032/150‑1 (MQ=255)
                                                                                                        aTGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGc                                          <  2:693840/150‑1 (MQ=255)
                                                                                                               aTCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTgg                                   >  1:268319/1‑150 (MQ=255)
                                                                                                               aTCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTgg                                   <  2:1239996/150‑1 (MQ=255)
                                                                                                                 cTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGaa                                 >  1:1347246/1‑150 (MQ=255)
                                                                                                                  tAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAAt                                <  2:431253/150‑1 (MQ=255)
                                                                                                                  tAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAAt                                >  2:1598247/1‑150 (MQ=255)
                                                                                                                   aaGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAAtt                               <  2:379981/150‑1 (MQ=255)
                                                                                                                    aGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAAttt                              >  2:1002919/1‑150 (MQ=255)
                                                                                                                    aGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAAttt                              <  2:2575007/150‑1 (MQ=255)
                                                                                                                    aGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAAttt                              >  2:2906286/1‑150 (MQ=255)
                                                                                                                    aGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAAttt                              >  2:2933243/1‑150 (MQ=255)
                                                                                                                      tCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAAttttt                            <  2:2287055/150‑1 (MQ=255)
                                                                                                                           aaaCACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTc                       >  1:1759218/1‑150 (MQ=255)
                                                                                                                            aaCACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCa                      >  2:3115371/1‑150 (MQ=255)
                                                                                                                            aaCACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCa                      <  2:3615223/150‑1 (MQ=255)
                                                                                                                             acacCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCAt                     <  1:397437/150‑1 (MQ=255)
                                                                                                                              caccacACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATg                    <  2:2042000/150‑1 (MQ=255)
                                                                                                                                  acacGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCaa                >  1:2707208/1‑150 (MQ=255)
                                                                                                                                    acgacgCTCGTGAGTAGACAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAAtt              <  2:3412209/150‑1 (MQ=255)
                                                                                                                                      gacgCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAg            >  2:127824/1‑150 (MQ=255)
                                                                                                                                        cgCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCg          >  2:1741916/1‑150 (MQ=255)
                                                                                                                                          cTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGc        <  1:2475010/150‑1 (MQ=255)
                                                                                                                                           tCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCa       <  2:1164985/150‑1 (MQ=255)
                                                                                                                                           tCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCa       <  1:3443001/150‑1 (MQ=255)
                                                                                                                                            cGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCaa      >  1:3154872/1‑150 (MQ=255)
                                                                                                                                            cGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCaa      >  2:729793/1‑150 (MQ=255)
                                                                                                                                                aGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGa  <  1:1949502/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGA  >  CP009273/2691757‑2692050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATA  >  CP009273/2691759‑2692053
                                                                                                                                                 |                                                                                                                                                     
CAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCA                                                                                                                                                   <  SRR19973061.524980/150‑1 (MQ=60)
CAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCA                                                                                                                                                   <  SRR19973061.1710114/150‑1 (MQ=60)
 AATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAA                                                                                                                                                  >  SRR19973061.402595/1‑150 (MQ=60)
 AATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAA                                                                                                                                                  >  SRR19973061.1730672/1‑150 (MQ=60)
 AATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAA                                                                                                                                                  >  SRR19973061.1730725/1‑150 (MQ=60)
  ATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAA                                                                                                                                                 >  SRR19973061.2235147/1‑150 (MQ=60)
    GGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACC                                                                                                                                               >  SRR19973061.2713643/1‑150 (MQ=60)
     GCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCA                                                                                                                                              >  SRR19973061.1776908/1‑150 (MQ=60)
          AATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTC                                                                                                                                         >  SRR19973061.1167935/1‑150 (MQ=60)
          AATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTC                                                                                                                                         >  SRR19973061.1167936/1‑150 (MQ=60)
          AATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTACCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTC                                                                                                                                         >  SRR19973061.3073475/1‑150 (MQ=60)
           ATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCC                                                                                                                                        >  SRR19973061.706708/1‑150 (MQ=60)
           ATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCC                                                                                                                                        <  SRR19973061.1176551/150‑1 (MQ=60)
            TCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCT                                                                                                                                       <  SRR19973061.2599394/150‑1 (MQ=60)
             CGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTC                                                                                                                                      <  SRR19973061.119033/150‑1 (MQ=60)
              GGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACCTATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGCCGCTCGTGAGTCGCCACACCATCCACCTCT                                                                                                                                     >  SRR19973061.1258201/1‑150 (MQ=60)
                TAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGG                                                                                                                                   >  SRR19973061.2425583/1‑150 (MQ=60)
                TAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGG                                                                                                                                   <  SRR19973061.713566/150‑1 (MQ=60)
                TAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGG                                                                                                                                   >  SRR19973061.2292838/1‑150 (MQ=60)
                 AACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGA                                                                                                                                  <  SRR19973061.2003381/150‑1 (MQ=60)
                   CAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACT                                                                                                                                >  SRR19973061.3624471/1‑150 (MQ=60)
                      CGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAA                                                                                                                             >  SRR19973061.425005/1‑150 (MQ=60)
                       GGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAA                                                                                                                            >  SRR19973061.2772310/1‑150 (MQ=60)
                         CAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACA                                                                                                                          >  SRR19973061.389543/1‑150 (MQ=60)
                         CAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACA                                                                                                                          >  SRR19973061.309559/1‑150 (MQ=60)
                               AAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGA                                                                                                                    <  SRR19973061.1586953/150‑1 (MQ=60)
                               AAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGA                                                                                                                    <  SRR19973061.767186/150‑1 (MQ=60)
                                AGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGAT                                                                                                                   >  SRR19973061.3239761/1‑150 (MQ=60)
                                   AACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAA                                                                                                                >  SRR19973061.1060203/1‑150 (MQ=60)
                                   AACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAA                                                                                                                >  SRR19973061.1668035/1‑150 (MQ=60)
                                   AACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCATGATTAA                                                                                                                >  SRR19973061.3269056/1‑150 (MQ=60)
                                    ACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAAT                                                                                                               <  SRR19973061.2711555/150‑1 (MQ=60)
                                       CATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTT                                                                                                            >  SRR19973061.2993094/1‑150 (MQ=60)
                                        ATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTC                                                                                                           <  SRR19973061.2444906/150‑1 (MQ=60)
                                        ATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTC                                                                                                           <  SRR19973061.2444925/150‑1 (MQ=60)
                                        ATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTC                                                                                                           >  SRR19973061.3150510/1‑150 (MQ=60)
                                        ATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTC                                                                                                           <  SRR19973061.3272064/150‑1 (MQ=60)
                                            AGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTA                                                                                                       <  SRR19973061.1561152/150‑1 (MQ=60)
                                            AGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTA                                                                                                       <  SRR19973061.1559873/150‑1 (MQ=60)
                                                GTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTAC                                                                                                   <  SRR19973061.2684367/150‑1 (MQ=60)
                                                   GGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATA                                                                                                <  SRR19973061.1857573/150‑1 (MQ=60)
                                                     CGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGC                                                                                              <  SRR19973061.1982083/150‑1 (MQ=60)
                                                     CGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGC                                                                                              <  SRR19973061.513769/150‑1 (MQ=60)
                                                        CGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTA                                                                                           <  SRR19973061.3361135/150‑1 (MQ=60)
                                                         GTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAA                                                                                          >  SRR19973061.68773/1‑150 (MQ=60)
                                                           AGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACA                                                                                        >  SRR19973061.1066219/1‑150 (MQ=60)
                                                            GTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAG                                                                                       >  SRR19973061.2772410/1‑150 (MQ=60)
                                                                ATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAG                                                                                   >  SRR19973061.435233/1‑150 (MQ=60)
                                                                ATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAG                                                                                   <  SRR19973061.2185181/150‑1 (MQ=60)
                                                                  AGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGAT                                                                                 <  SRR19973061.1136271/150‑1 (MQ=60)
                                                                     GCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCAT                                                                              <  SRR19973061.385099/150‑1 (MQ=60)
                                                                     GCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCAT                                                                              <  SRR19973061.385090/150‑1 (MQ=60)
                                                                     GCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCAT                                                                              <  SRR19973061.384091/150‑1 (MQ=60)
                                                                       AGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCA                                                                            >  SRR19973061.3478925/1‑150 (MQ=60)
                                                                       AGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCA                                                                            <  SRR19973061.190499/150‑1 (MQ=60)
                                                                         AAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACT                                                                          <  SRR19973061.1192110/150‑1 (MQ=60)
                                                                         AAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACT                                                                          >  SRR19973061.2238975/1‑150 (MQ=60)
                                                                           AACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTT                                                                        <  SRR19973061.1358023/150‑1 (MQ=60)
                                                                             CTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTC                                                                      <  SRR19973061.2469939/150‑1 (MQ=60)
                                                                                   AACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAA                                                                <  SRR19973061.1172135/150‑1 (MQ=60)
                                                                                   AACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAA                                                                >  SRR19973061.917268/1‑150 (MQ=60)
                                                                                   AACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAA                                                                >  SRR19973061.1664612/1‑150 (MQ=60)
                                                                                     CATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACA                                                              <  SRR19973061.1604427/150‑1 (MQ=60)
                                                                                      ATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAA                                                             >  SRR19973061.138223/1‑150 (MQ=60)
                                                                                      ATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAA                                                             <  SRR19973061.832244/150‑1 (MQ=60)
                                                                                      ATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAA                                                             >  SRR19973061.139068/1‑150 (MQ=60)
                                                                                      ATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAA                                                             >  SRR19973061.1607136/1‑150 (MQ=60)
                                                                                       TATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAAT                                                            >  SRR19973061.2517737/1‑150 (MQ=60)
                                                                                        ATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATC                                                           >  SRR19973061.3335335/1‑150 (MQ=60)
                                                                                         TCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCG                                                          <  SRR19973061.883110/150‑1 (MQ=60)
                                                                                          CCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGA                                                         >  SRR19973061.1596647/1‑150 (MQ=60)
                                                                                          CCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGAAGCACGTGAGAAGCCAAACCATACTCCTCTGGACTGAAACATCCTGAATAATTTACGCTACTACATAACCAAACAGATAGATCATCACTTTTCCGGCAACAATCGA                                                         >  SRR19973061.1242017/1‑150 (MQ=60)
                                                                                           CTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGAC                                                        >  SRR19973061.3249176/1‑150 (MQ=60)
                                                                                            TGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACC                                                       <  SRR19973061.3025793/150‑1 (MQ=60)
                                                                                            TGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACC                                                       <  SRR19973061.2501947/150‑1 (MQ=60)
                                                                                            TGCTGATGCAATGTTCCATATAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACC                                                       <  SRR19973061.3028979/150‑1 (MQ=60)
                                                                                               TGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCA                                                    >  SRR19973061.1793195/1‑150 (MQ=60)
                                                                                               TGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCA                                                    >  SRR19973061.3069458/1‑150 (MQ=60)
                                                                                                GATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCAT                                                   >  SRR19973061.145471/1‑150 (MQ=60)
                                                                                                GATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCAT                                                   >  SRR19973061.145415/1‑150 (MQ=60)
                                                                                                   GCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCAT                                                <  SRR19973061.2849313/150‑1 (MQ=60)
                                                                                                    CAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATG                                               >  SRR19973061.3155493/1‑150 (MQ=60)
                                                                                                    AAATGTTCCATCTAATTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATG                                               <  SRR19973061.270697/150‑1 (MQ=60)
                                                                                                         TTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGATACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAA                                          <  SRR19973061.2279159/150‑1 (MQ=60)
                                                                                                             ATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGG                                      >  SRR19973061.269056/1‑150 (MQ=60)
                                                                                                               CTAAGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAA                                    >  SRR19973061.1350636/1‑150 (MQ=60)
                                                                                                                  AGTCAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTT                                 >  SRR19973061.2567824/1‑150 (MQ=60)
                                                                                                                   ttaAAAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTT                                <  SRR19973061.988917/147‑1 (MQ=60)
                                                                                                                       AAAAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAAT                            <  SRR19973061.1720765/150‑1 (MQ=60)
                                                                                                                         AAACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTC                          >  SRR19973061.1763623/1‑150 (MQ=60)
                                                                                                                           ACACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCAT                        <  SRR19973061.398524/150‑1 (MQ=60)
                                                                                                                            CACCACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTGTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATG                       <  SRR19973061.1957171/150‑1 (MQ=60)
                                                                                                                             ACCACACGACGATCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGG                      <  SRR19973061.264525/150‑1 (MQ=60)
                                                                                                                                ACACGACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAA                   >  SRR19973061.2714173/1‑150 (MQ=60)
                                                                                                                                     ACGCTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGC              >  SRR19973061.872880/1‑150 (MQ=60)
                                                                                                                                        CTCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGC           <  SRR19973061.2481310/150‑1 (MQ=60)
                                                                                                                                         TCGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCA          <  SRR19973061.3451836/150‑1 (MQ=60)
                                                                                                                                          CGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAA         >  SRR19973061.3162980/1‑150 (MQ=60)
                                                                                                                                          CGTGAGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAA         >  SRR19973061.213645/1‑150 (MQ=60)
                                                                                                                                              AGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGA     <  SRR19973061.777391/150‑1 (MQ=60)
                                                                                                                                              AGTAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGA     <  SRR19973061.1954366/150‑1 (MQ=60)
                                                                                                                                                TAGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAAT   >  SRR19973061.3670764/1‑150 (MQ=60)
                                                                                                                                                 AGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATA  >  SRR19973061.3123222/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
CAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATA  >  CP009273/2691759‑2692053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑