Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 3,122,778 A→C pseudogene (725/1101 nt) yghO ← pseudogene, DNA‑binding transcriptional regulator homology

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,122,7780AC99.5% 804.4 / NA 204pseudogene (725/1101 nt)yghOpseudogene, DNA‑binding transcriptional regulator homology
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (116/87);  total (116/88)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

GTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGA  >  CP009273/3122631‑3122924
                                                                                                                                                   |                                                                                                                                                  
gTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTc                                                                                                                                                  <  2:1054737/150‑1 (MQ=255)
    gaacacCGCTTACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCatat                                                                                                                                              <  2:3432672/148‑1 (MQ=255)
     cacaCCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATc                                                                                                                                             <  2:769561/150‑1 (MQ=255)
     cacaCCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATc                                                                                                                                             >  1:2547769/1‑150 (MQ=255)
        acCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTc                                                                                                                                          <  2:2596481/150‑1 (MQ=255)
          cGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCg                                                                                                                                        >  1:2588277/1‑150 (MQ=255)
           gCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCgg                                                                                                                                       <  1:3439058/150‑1 (MQ=255)
           gCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCgg                                                                                                                                       <  1:323388/150‑1 (MQ=255)
                cTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCa                                                                                                                                  <  1:130287/150‑1 (MQ=255)
                    cAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGata                                                                                                                              >  2:2130600/1‑150 (MQ=255)
                      aaCGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATAtt                                                                                                                            <  1:890833/150‑1 (MQ=255)
                      aaCGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATAtt                                                                                                                            <  2:2199308/150‑1 (MQ=255)
                           cacaGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGt                                                                                                                       >  1:332232/1‑150 (MQ=255)
                             caGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTg                                                                                                                     >  1:1633370/1‑150 (MQ=255)
                             caGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTg                                                                                                                     <  1:3134322/150‑1 (MQ=255)
                               gCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGAt                                                                                                                   <  1:3378185/150‑1 (MQ=255)
                                cAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATc                                                                                                                  <  2:1459036/150‑1 (MQ=255)
                                cAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATc                                                                                                                  <  2:1994451/150‑1 (MQ=255)
                                  aTTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGc                                                                                                                >  1:511306/1‑150 (MQ=255)
                                    tttGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGccc                                                                                                              >  2:2885271/1‑150 (MQ=255)
                                    tttGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGccc                                                                                                              <  1:1991288/150‑1 (MQ=255)
                                    tttGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGccc                                                                                                              >  1:1746773/1‑150 (MQ=255)
                                      tGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCAt                                                                                                            <  1:388835/150‑1 (MQ=255)
                                      tGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCCCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCAt                                                                                                            <  1:1465579/150‑1 (MQ=255)
                                        cccAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATgg                                                                                                          >  2:2356442/1‑150 (MQ=255)
                                           aGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTcg                                                                                                       >  2:1243881/1‑150 (MQ=255)
                                           aGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTcg                                                                                                       <  1:754459/150‑1 (MQ=255)
                                           aGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTcg                                                                                                       <  2:3605098/150‑1 (MQ=255)
                                             ccGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTcgcg                                                                                                     >  2:2231214/1‑150 (MQ=255)
                                             ccGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTcgcg                                                                                                     >  2:3497853/1‑150 (MQ=255)
                                               gAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGaa                                                                                                   <  2:3715586/150‑1 (MQ=255)
                                               gAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGaa                                                                                                   >  2:160761/1‑150 (MQ=255)
                                               gAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGaa                                                                                                   >  2:160817/1‑150 (MQ=255)
                                                aaGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAt                                                                                                  >  2:728453/1‑150 (MQ=255)
                                                aaGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAt                                                                                                  <  1:455784/150‑1 (MQ=255)
                                                    gAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCAt                                                                                              <  2:1433398/150‑1 (MQ=255)
                                                    gAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCAt                                                                                              <  1:355662/150‑1 (MQ=255)
                                                       agagCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGtt                                                                                           <  2:3449297/150‑1 (MQ=255)
                                                         agCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCg                                                                                         <  2:131038/150‑1 (MQ=255)
                                                         agCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCg                                                                                         <  2:2588277/150‑1 (MQ=255)
                                                          gCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCgg                                                                                        <  1:3188091/150‑1 (MQ=255)
                                                               ccGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAAc                                                                                   >  1:2245848/1‑150 (MQ=255)
                                                                  ttCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATATCTCATATCGTCCGGCACCAGATATTTAAGTTTATCGCAAATTGTAGCGAATTCATGTTCGGTAAACGGc                                                                                >  1:2429156/1‑150 (MQ=255)
                                                                  ttCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGc                                                                                >  1:1344225/1‑150 (MQ=255)
                                                                     aGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGcaca                                                                             >  2:675052/1‑150 (MQ=255)
                                                                     aGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGcaca                                                                             <  2:332232/150‑1 (MQ=255)
                                                                      gATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACaa                                                                            >  2:1821835/1‑150 (MQ=255)
                                                                          ggCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATcc                                                                        >  1:133616/1‑150 (MQ=255)
                                                                            cAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATcccc                                                                      >  2:1558268/1‑150 (MQ=255)
                                                                             aaTCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCa                                                                     <  1:2856593/150‑1 (MQ=255)
                                                                              aTCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAg                                                                    >  2:3246137/1‑150 (MQ=255)
                                                                              aTCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAg                                                                    >  2:2033925/1‑150 (MQ=255)
                                                                                cGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGtt                                                                  >  1:3177138/1‑150 (MQ=255)
                                                                                 gCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTtg                                                                 <  1:770086/150‑1 (MQ=255)
                                                                                     cGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgct                                                             >  1:3005629/1‑150 (MQ=255)
                                                                                      gTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgctg                                                            >  2:3119902/1‑150 (MQ=255)
                                                                                        tGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAAACAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgcc                                                          >  1:2008609/1‑150 (MQ=255)
                                                                                         gATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgcca                                                         <  2:1336010/150‑1 (MQ=255)
                                                                                          aTGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccag                                                        <  1:3798745/150‑1 (MQ=255)
                                                                                            gTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagcc                                                      <  1:1121249/150‑1 (MQ=255)
                                                                                            gTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagcc                                                      <  2:481674/150‑1 (MQ=255)
                                                                                               cGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCaga                                                   >  1:3732910/1‑150 (MQ=255)
                                                                                                ggCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCagag                                                  <  2:1273593/150‑1 (MQ=255)
                                                                                                ggCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCagag                                                  >  2:1113304/1‑150 (MQ=255)
                                                                                                ggCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCagag                                                  <  2:569380/150‑1 (MQ=255)
                                                                                                   aaGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAATGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTg                                               <  1:2816661/150‑1 (MQ=255)
                                                                                                    aGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGa                                              >  1:1792555/1‑150 (MQ=255)
                                                                                                       cGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGaaaa                                           >  1:418804/1‑150 (MQ=255)
                                                                                                          cAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtct                                        <  1:1047911/150‑1 (MQ=255)
                                                                                                          cAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtct                                        >  2:2717257/1‑150 (MQ=255)
                                                                                                          cAATAAACGAGCATGGTGCAGAATAAATCTCAGCGATATAGATCATATAGTCCGGCACCAGATATTTAAGTTGATCGACCATGGTCGCGTATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtct                                        <  1:2951502/150‑1 (MQ=255)
                                                                                                                 cgcgCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAg                                 <  2:2485480/150‑1 (MQ=255)
                                                                                                                   cgcAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGa                               <  2:1940258/150‑1 (MQ=255)
                                                                                                                   cgcAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGa                               >  1:1676969/1‑150 (MQ=255)
                                                                                                                   cgcAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGATGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGa                               >  1:659165/1‑150 (MQ=255)
                                                                                                                    gcAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGAt                              <  2:1162572/150‑1 (MQ=255)
                                                                                                                      aGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCt                            <  1:1712087/150‑1 (MQ=255)
                                                                                                                       gggTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTg                           <  2:1682862/150‑1 (MQ=255)
                                                                                                                       gggTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTg                           <  2:3663612/150‑1 (MQ=255)
                                                                                                                        ggTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGc                          >  1:2631850/1‑150 (MQ=255)
                                                                                                                         gTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCa                         <  1:3708177/150‑1 (MQ=255)
                                                                                                                             aGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAtctc                     >  1:3157592/1‑150 (MQ=255)
                                                                                                                              gAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATctct                    <  1:1829951/150‑1 (MQ=255)
                                                                                                                              gAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATctct                    <  2:1109395/150‑1 (MQ=255)
                                                                                                                              gAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGAGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATctct                    >  2:1709272/1‑150 (MQ=255)
                                                                                                                                atcaatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTc                  <  1:3764478/150‑1 (MQ=255)
                                                                                                                                atcaatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTc                  <  1:2356442/150‑1 (MQ=255)
                                                                                                                                atcaatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTc                  <  2:632944/150‑1 (MQ=255)
                                                                                                                                  caatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAATTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCgg                >  2:2119224/1‑150 (MQ=255)
                                                                                                                                   aatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGc               >  1:2831035/1‑150 (MQ=255)
                                                                                                                                    atcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCa              >  2:50714/1‑150 (MQ=255)
                                                                                                                                    atcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCa              >  1:2084962/1‑150 (MQ=255)
                                                                                                                                      ctcAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCaaa            >  2:2955493/1‑150 (MQ=255)
                                                                                                                                         aGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCg         >  2:301566/1‑150 (MQ=255)
                                                                                                                                           cGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCt       >  2:3106024/1‑150 (MQ=255)
                                                                                                                                             atatAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGa     >  1:2899064/1‑150 (MQ=255)
                                                                                                                                             atatAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGa     <  1:1593248/150‑1 (MQ=255)
                                                                                                                                             atatAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGa     <  2:2495852/150‑1 (MQ=255)
                                                                                                                                              tataGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAc    >  1:3035447/1‑150 (MQ=255)
                                                                                                                                              tataGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAc    >  1:578048/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGA  >  CP009273/3122631‑3122924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC  >  CP009273/3122630‑3122922
                                                                                                                                                    |                                                                                                                                                
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCT                                                                                                                                                 >  SRR19973061.1503176/1‑150 (MQ=60)
   TCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCAT                                                                                                                                              >  SRR19973061.866716/1‑150 (MQ=60)
   TCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCAT                                                                                                                                              >  SRR19973061.1214003/1‑150 (MQ=60)
   TCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCAT                                                                                                                                              <  SRR19973061.28759/150‑1 (MQ=60)
     GCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATAT                                                                                                                                            <  SRR19973061.373089/150‑1 (MQ=60)
      CACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATC                                                                                                                                           <  SRR19973061.1317179/150‑1 (MQ=60)
      CACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATC                                                                                                                                           >  SRR19973061.2554249/1‑150 (MQ=60)
      CACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATC                                                                                                                                           <  SRR19973061.1383170/150‑1 (MQ=60)
         ACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTC                                                                                                                                        <  SRR19973061.602346/150‑1 (MQ=60)
           CGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCG                                                                                                                                      >  SRR19973061.1671423/1‑150 (MQ=60)
           CGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCG                                                                                                                                      >  SRR19973061.2594906/1‑150 (MQ=60)
           CGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCG                                                                                                                                      >  SRR19973061.1517827/1‑150 (MQ=60)
            GCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGG                                                                                                                                     >  SRR19973061.2232501/1‑150 (MQ=60)
            GCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGG                                                                                                                                     <  SRR19973061.3447880/150‑1 (MQ=60)
            GCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGG                                                                                                                                     <  SRR19973061.324233/150‑1 (MQ=60)
            GCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGG                                                                                                                                     <  SRR19973061.323640/150‑1 (MQ=60)
             CTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGC                                                                                                                                    >  SRR19973061.1347560/1‑150 (MQ=60)
             CTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGC                                                                                                                                    >  SRR19973061.2502208/1‑150 (MQ=60)
              TGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCA                                                                                                                                   <  SRR19973061.2311149/150‑1 (MQ=60)
                 CTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCA                                                                                                                                <  SRR19973061.130704/150‑1 (MQ=60)
                     CAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATA                                                                                                                            >  SRR19973061.570867/1‑150 (MQ=60)
                       AACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATT                                                                                                                          <  SRR19973061.893134/150‑1 (MQ=60)
                           CCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAG                                                                                                                      >  SRR19973061.341524/1‑150 (MQ=60)
                            CACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGT                                                                                                                     >  SRR19973061.333107/1‑150 (MQ=60)
                            CACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGT                                                                                                                     >  SRR19973061.332225/1‑150 (MQ=60)
                              CAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTG                                                                                                                   <  SRR19973061.2567948/150‑1 (MQ=60)
                              CAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTG                                                                                                                   <  SRR19973061.3142394/150‑1 (MQ=60)
                              CAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTG                                                                                                                   >  SRR19973061.1637459/1‑150 (MQ=60)
                               AGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGA                                                                                                                  <  SRR19973061.387521/150‑1 (MQ=60)
                                GCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGAT                                                                                                                 <  SRR19973061.3386867/150‑1 (MQ=60)
                                 CGATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATC                                                                                                                <  SRR19973061.3151814/150‑1 (MQ=60)
                                 CAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATC                                                                                                                <  SRR19973061.2952610/150‑1 (MQ=60)
                                   ATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGC                                                                                                              >  SRR19973061.512657/1‑150 (MQ=60)
                                    TTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCC                                                                                                             <  SRR19973061.2834332/150‑1 (MQ=60)
                                     TTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCC                                                                                                            <  SRR19973061.1996273/150‑1 (MQ=60)
                                     TTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCC                                                                                                            >  SRR19973061.1751161/1‑150 (MQ=60)
                                       TGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCAT                                                                                                          <  SRR19973061.389898/150‑1 (MQ=60)
                                       TGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCCCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCAT                                                                                                          <  SRR19973061.1469268/150‑1 (MQ=60)
                                            AGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCG                                                                                                     <  SRR19973061.756442/150‑1 (MQ=60)
                                             GCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGC                                                                                                    <  SRR19973061.79336/150‑1 (MQ=60)
                                                 AAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAT                                                                                                <  SRR19973061.457015/150‑1 (MQ=60)
                                                     GAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCAT                                                                                            <  SRR19973061.356621/150‑1 (MQ=60)
                                                        AGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTT                                                                                         >  SRR19973061.2973321/1‑150 (MQ=60)
                                                         GAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTC                                                                                        >  SRR19973061.3510252/1‑150 (MQ=60)
                                                           GCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGG                                                                                      <  SRR19973061.3196272/150‑1 (MQ=60)
                                                           GCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGG                                                                                      <  SRR19973061.3196248/150‑1 (MQ=60)
                                                                CCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAAC                                                                                 >  SRR19973061.2251493/1‑150 (MQ=60)
                                                                 CGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACG                                                                                <  SRR19973061.2615577/150‑1 (MQ=60)
                                                                   TTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATATCTCATATCGTCCGGCACCAGATATTTAAGTTTATCGCAAATTGTAGCGAATTCATGTTCGGTAAACGGC                                                                              >  SRR19973061.2435358/1‑150 (MQ=60)
                                                                   TTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGC                                                                              >  SRR19973061.1347604/1‑150 (MQ=60)
                                                                         TCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAAT                                                                        <  SRR19973061.2001524/150‑1 (MQ=60)
                                                                           GGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCC                                                                      >  SRR19973061.134045/1‑150 (MQ=60)
                                                                              AATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCA                                                                   <  SRR19973061.2863907/150‑1 (MQ=60)
                                                                              AATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCA                                                                   <  SRR19973061.2862451/150‑1 (MQ=60)
                                                                                 CGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTT                                                                >  SRR19973061.268187/1‑150 (MQ=60)
                                                                                 CGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTT                                                                >  SRR19973061.3185287/1‑150 (MQ=60)
                                                                                  GCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTG                                                               <  SRR19973061.429180/150‑1 (MQ=60)
                                                                                  GCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTG                                                               <  SRR19973061.772106/150‑1 (MQ=60)
                                                                                    CTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTG                                                             >  SRR19973061.3028405/1‑150 (MQ=60)
                                                                                    CTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTG                                                             >  SRR19973061.488290/1‑150 (MQ=60)
                                                                                      CGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCT                                                           >  SRR19973061.3013388/1‑150 (MQ=60)
                                                                                      CGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCT                                                           >  SRR19973061.1618359/1‑150 (MQ=60)
                                                                                       GTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTG                                                          >  SRR19973061.338074/1‑150 (MQ=60)
                                                                                         TGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAAACAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCC                                                        >  SRR19973061.2013651/1‑150 (MQ=60)
                                                                                           ATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAG                                                      <  SRR19973061.3808429/150‑1 (MQ=60)
                                                                                           ATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAG                                                      >  SRR19973061.306132/1‑150 (MQ=60)
                                                                                             GTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCC                                                    <  SRR19973061.1124115/150‑1 (MQ=60)
                                                                                             GTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGATGTGCTGCCAGCC                                                    >  SRR19973061.2028891/1‑150 (MQ=60)
                                                                                                CGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGA                                                 >  SRR19973061.3743221/1‑150 (MQ=60)
                                                                                                CGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGA                                                 >  SRR19973061.3742460/1‑150 (MQ=60)
                                                                                                   CAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTT                                              >  SRR19973061.395407/1‑150 (MQ=60)
                                                                                                    AAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAATGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTG                                             <  SRR19973061.2823889/150‑1 (MQ=60)
                                                                                                    AAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTG                                             >  SRR19973061.1876085/1‑150 (MQ=60)
                                                                                                    AAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTG                                             >  SRR19973061.3775613/1‑150 (MQ=60)
                                                                                                     AGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGA                                            >  SRR19973061.1797039/1‑150 (MQ=60)
                                                                                                        CGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAA                                         >  SRR19973061.419940/1‑150 (MQ=60)
                                                                                                          ACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATC                                       <  SRR19973061.3591824/150‑1 (MQ=60)
                                                                                                           CAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCT                                      <  SRR19973061.1050595/150‑1 (MQ=60)
                                                                                                           CAATAAACGAGCATGGTGCAGAATAAATCTCAGCGATATAGATCATATAGTCCGGCACCAGATATTTAAGTTGATCGACCATGGTCGCGTATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCT                                      <  SRR19973061.2959113/150‑1 (MQ=60)
                                                                                                                 ACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCA                                >  SRR19973061.723425/1‑150 (MQ=60)
                                                                                                                    CGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGA                             >  SRR19973061.1681180/1‑150 (MQ=60)
                                                                                                                    CGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGA                             >  SRR19973061.1681323/1‑150 (MQ=60)
                                                                                                                    CGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGATGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGA                             >  SRR19973061.660858/1‑150 (MQ=60)
                                                                                                                       AGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCT                          >  SRR19973061.2363913/1‑150 (MQ=60)
                                                                                                                       AGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCT                          <  SRR19973061.1716388/150‑1 (MQ=60)
                                                                                                                         GGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGC                        >  SRR19973061.2638613/1‑150 (MQ=60)
                                                                                                                          GTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCA                       <  SRR19973061.3717657/150‑1 (MQ=60)
                                                                                                                             CAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCT                    <  SRR19973061.953614/150‑1 (MQ=60)
                                                                                                                              AGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTC                   >  SRR19973061.3166012/1‑150 (MQ=60)
                                                                                                                              AGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTC                   >  SRR19973061.3165706/1‑150 (MQ=60)
                                                                                                                              AGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTC                   >  SRR19973061.1768537/1‑150 (MQ=60)
                                                                                                                               GAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCT                  <  SRR19973061.1834537/150‑1 (MQ=60)
                                                                                                                               GAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCT                  <  SRR19973061.1804201/150‑1 (MQ=60)
                                                                                                                                 ATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTC                <  SRR19973061.2362429/150‑1 (MQ=60)
                                                                                                                                 ATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTC                <  SRR19973061.3774097/150‑1 (MQ=60)
                                                                                                                                    AATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGC             >  SRR19973061.2838285/1‑150 (MQ=60)
                                                                                                                                     ATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCA            >  SRR19973061.2090191/1‑150 (MQ=60)
                                                                                                                                            CGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCT     >  SRR19973061.1747424/1‑150 (MQ=60)
                                                                                                                                              ATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGA   >  SRR19973061.2906503/1‑150 (MQ=60)
                                                                                                                                              ATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGA   >  SRR19973061.2906379/1‑150 (MQ=60)
                                                                                                                                              ATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGA   <  SRR19973061.1597201/150‑1 (MQ=60)
                                                                                                                                               TATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC  <  SRR19973061.769918/150‑1 (MQ=60)
                                                                                                                                               TATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC  >  SRR19973061.579556/1‑150 (MQ=60)
                                                                                                                                               TATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC  >  SRR19973061.3043291/1‑150 (MQ=60)
                                                                                                                                               TATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC  >  SRR19973061.1087100/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC  >  CP009273/3122630‑3122922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑