Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,122,778 A→C 100% pseudogene (725/1101 nt) yghO ← pseudogene, DNA‑binding transcriptional regulator homology

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,122,7780AC98.5% 856.9 / ‑2.1 213pseudogene (725/1101 nt)yghOpseudogene, DNA‑binding transcriptional regulator homology
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base C (103/106);  total (105/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 213 total aligned reads displayed.

TCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC  >  CP009273/3122633‑3122922
                                                                                                                                                 |                                                                                                                                                
   cacaCCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCata                                                                                                                                             <  1:2900410/148‑1 (MQ=255)
    acacCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCatat                                                                                                                                            <  1:226120/148‑1 (MQ=255)
      acCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCg                                                                                                                                          >  2:1783145/1‑148 (MQ=255)
       ccGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGt                                                                                                                                         <  2:3331156/148‑1 (MQ=255)
       ccGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGt                                                                                                                                         <  1:2789109/148‑1 (MQ=255)
       ccGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGACAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGt                                                                                                                                         >  1:3518762/1‑148 (MQ=255)
        cGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTc                                                                                                                                        <  1:1531364/148‑1 (MQ=255)
            gACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGt                                                                                                                                         >  2:236274/1‑143 (MQ=255)
            gACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGt                                                                                                                                         <  1:236274/143‑1 (MQ=255)
             aCTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCa                                                                                                                                   >  1:1245590/1‑148 (MQ=255)
                   aatcGCCACAGCAATTTTGCACAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCGTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCTATCTCAGCGATATAGCTCATATCGTCCGTCACCAGat                                                                                                                             <  2:716044/145‑1 (MQ=255)
                    aaCGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGata                                                                                                                            <  1:3093375/148‑1 (MQ=255)
                     aCGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGatat                                                                                                                           <  1:3730343/148‑1 (MQ=255)
                     aCGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGatat                                                                                                                           >  1:3776587/1‑148 (MQ=255)
                      cGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATTCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATAtt                                                                                                                          >  2:1568752/1‑148 (MQ=255)
                      cGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCatat                                                                                                                                            <  1:2217891/130‑1 (MQ=255)
                      cGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCatat                                                                                                                                            >  2:2217891/1‑130 (MQ=255)
                      cGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATAtt                                                                                                                          >  1:3418554/1‑148 (MQ=255)
                       gCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATAttt                                                                                                                         <  1:297903/148‑1 (MQ=255)
                        ccACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTa                                                                                                                        <  1:38739/148‑1 (MQ=255)
                         cacaGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTaa                                                                                                                       <  1:135852/148‑1 (MQ=255)
                         cacaGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTaa                                                                                                                       >  2:1009923/1‑148 (MQ=255)
                          acaGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAg                                                                                                                      <  2:2495888/148‑1 (MQ=255)
                           caGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGt                                                                                                                     >  1:2057482/1‑148 (MQ=255)
                             gCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTg                                                                                                                   <  1:2151010/148‑1 (MQ=255)
                             gCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTg                                                                                                                   <  1:2148245/148‑1 (MQ=255)
                              cAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGa                                                                                                                  <  2:1058410/148‑1 (MQ=255)
                              cAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGa                                                                                                                  <  2:339258/148‑1 (MQ=255)
                                aTTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATc                                                                                                                >  2:672280/1‑148 (MQ=255)
                                 ttttGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCg                                                                                                               <  2:3571758/148‑1 (MQ=255)
                                  tttGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGc                                                                                                              <  2:2360294/148‑1 (MQ=255)
                                   ttGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGcc                                                                                                             <  2:1607889/148‑1 (MQ=255)
                                      cccAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCAt                                                                                                          <  2:1677701/148‑1 (MQ=255)
                                           ccGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTcg                                                                                                     >  2:1570723/1‑148 (MQ=255)
                                           ccGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTcg                                                                                                     >  2:1497966/1‑148 (MQ=255)
                                              aaGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGa                                                                                                  >  1:552579/1‑148 (MQ=255)
                                               aGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGaa                                                                                                 >  1:1757755/1‑148 (MQ=255)
                                               aGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGaa                                                                                                 <  2:3141982/148‑1 (MQ=255)
                                                gggAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAt                                                                                                >  2:296365/1‑148 (MQ=255)
                                                 ggAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAtt                                                                                               <  1:375292/148‑1 (MQ=255)
                                                 ggAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAtt                                                                                               <  1:1153432/148‑1 (MQ=255)
                                                   aaaGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCa                                                                                             <  2:1333557/148‑1 (MQ=255)
                                                     agagCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATg                                                                                           >  2:641981/1‑148 (MQ=255)
                                                     agagCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATg                                                                                           >  1:1510490/1‑148 (MQ=255)
                                                      gagCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGt                                                                                          <  2:3696558/148‑1 (MQ=255)
                                                      gagCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGt                                                                                          >  2:1980650/1‑148 (MQ=255)
                                                       agCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGtt                                                                                         <  2:1932511/148‑1 (MQ=255)
                                                       agCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGtt                                                                                         <  2:1273750/148‑1 (MQ=255)
                                                       agCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGtt                                                                                         >  2:1118669/1‑148 (MQ=255)
                                                         cGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATCAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCg                                                                                       >  2:2613423/1‑148 (MQ=255)
                                                         cGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCg                                                                                       >  1:2759622/1‑148 (MQ=255)
                                                         cGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCg                                                                                       >  2:696567/1‑148 (MQ=255)
                                                           aTCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGt                                                                                     >  1:1905446/1‑148 (MQ=255)
                                                            tCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTa                                                                                    >  1:2599479/1‑148 (MQ=255)
                                                              cGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTaaa                                                                                  <  2:1850908/148‑1 (MQ=255)
                                                               gTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAAc                                                                                 <  2:3250069/148‑1 (MQ=255)
                                                                   aGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGACATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGca                                                                             <  2:1056920/148‑1 (MQ=255)
                                                                    gATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGcac                                                                            >  1:171003/1‑148 (MQ=255)
                                                                     aTCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGcaca                                                                           >  1:3622597/1‑148 (MQ=255)
                                                                        ggCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAAt                                                                        <  1:1713348/148‑1 (MQ=255)
                                                                        ggCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAAt                                                                        <  1:2815791/148‑1 (MQ=255)
                                                                            aTCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATcccc                                                                    <  1:1037341/148‑1 (MQ=255)
                                                                             tCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCa                                                                   <  2:2606473/148‑1 (MQ=255)
                                                                             tCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCa                                                                   <  2:3232508/148‑1 (MQ=255)
                                                                             tCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCa                                                                   >  2:1952876/1‑148 (MQ=255)
                                                                              cGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAg                                                                  >  1:1573505/1‑148 (MQ=255)
                                                                              cGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAg                                                                  >  1:2386389/1‑148 (MQ=255)
                                                                               gCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAAAGGCACAAATCACCAGt                                                                 <  1:1309803/148‑1 (MQ=255)
                                                                                ccTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGtt                                                                <  2:2072477/148‑1 (MQ=255)
                                                                                     ttGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgct                                                           >  1:3568198/1‑148 (MQ=255)
                                                                                      tGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgctg                                                          >  1:2571160/1‑148 (MQ=255)
                                                                                      tGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgctg                                                          <  1:3278037/148‑1 (MQ=255)
                                                                                      tGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgctg                                                          >  1:3152272/1‑148 (MQ=255)
                                                                                      tGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCCCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgctg                                                          >  1:2912163/1‑148 (MQ=255)
                                                                                           ttCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagc                                                     <  2:552579/148‑1 (MQ=255)
                                                                                              ggCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGccagccag                                                  <  2:3622597/148‑1 (MQ=255)
                                                                                                cAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCCGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCagag                                                <  1:379052/148‑1 (MQ=255)
                                                                                                cAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCagag                                                >  1:240685/1‑148 (MQ=255)
                                                                                                 aaGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGt                                               <  2:3528636/148‑1 (MQ=255)
                                                                                                   gCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTg                                             >  1:2987070/1‑148 (MQ=255)
                                                                                                           tAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtctc                                     <  1:1532659/148‑1 (MQ=255)
                                                                                                           tAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtctc                                     <  1:2472968/148‑1 (MQ=255)
                                                                                                              aCGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACg                                  >  2:3501391/1‑148 (MQ=255)
                                                                                                               cgcgCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGc                                 <  1:1387280/148‑1 (MQ=255)
                                                                                                                gcgcAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCa                                <  1:1444740/148‑1 (MQ=255)
                                                                                                                   cAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGa                             >  1:18354/1‑148 (MQ=255)
                                                                                                                        tGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGc                        >  2:865846/1‑148 (MQ=255)
                                                                                                                          cAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAt                      >  2:2005297/1‑148 (MQ=255)
                                                                                                                          cAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAt                      >  2:3757789/1‑148 (MQ=255)
                                                                                                                            gAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAtct                    >  2:2882229/1‑148 (MQ=255)
                                                                                                                             aatcaatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAtctc                   >  2:3027730/1‑148 (MQ=255)
                                                                                                                             aatcaatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAtctc                   >  2:1373/1‑148 (MQ=255)
                                                                                                                                caatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTc                >  2:2517302/1‑148 (MQ=255)
                                                                                                                                caatcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTc                >  2:818205/1‑148 (MQ=255)
                                                                                                                                  atcTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCgg              >  2:1011789/1‑148 (MQ=255)
                                                                                                                                         cGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCg       >  2:1952067/1‑148 (MQ=255)
                                                                                                                                            tataGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTg    >  2:2387504/1‑148 (MQ=255)
                                                                                                                                            tataGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTg    <  2:1460746/148‑1 (MQ=255)
                                                                                                                                             ataGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGa   >  1:101915/1‑148 (MQ=255)
                                                                                                                                              taGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAc  >  1:751470/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC  >  CP009273/3122633‑3122922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTG  >  CP009273/3122630‑3122927
                                                                                                                                                    |                                                                                                                                                     
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCT                                                                                                                                                      <  SRR19973069.91320/150‑1 (MQ=60)
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCT                                                                                                                                                      <  SRR19973069.2369149/150‑1 (MQ=60)
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCT                                                                                                                                                      <  SRR19973069.2369101/150‑1 (MQ=60)
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCT                                                                                                                                                      <  SRR19973069.2368672/150‑1 (MQ=60)
  TTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCA                                                                                                                                                    <  SRR19973069.3530910/150‑1 (MQ=60)
   TCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCAT                                                                                                                                                   <  SRR19973069.3034727/150‑1 (MQ=60)
    CGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATA                                                                                                                                                  >  SRR19973069.99650/1‑150 (MQ=60)
      CACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCCAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATC                                                                                                                                                <  SRR19973069.3593624/150‑1 (MQ=60)
      CACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATC                                                                                                                                                <  SRR19973069.2919143/150‑1 (MQ=60)
      CACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATC                                                                                                                                                <  SRR19973069.2919562/150‑1 (MQ=60)
      CACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATC                                                                                                                                                <  SRR19973069.2919820/150‑1 (MQ=60)
       tgtgctcttccgatctAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCG                                                                                                                                               <  SRR19973069.2232899/134‑1 (MQ=60)
       ACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCG                                                                                                                                               <  SRR19973069.3685266/150‑1 (MQ=60)
       ACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCG                                                                                                                                               <  SRR19973069.227863/150‑1 (MQ=60)
        CACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGACAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGT                                                                                                                                              >  SRR19973069.3541472/1‑150 (MQ=60)
        CACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGACAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGT                                                                                                                                              >  SRR19973069.3541154/1‑150 (MQ=60)
          tctCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCC                                                                                                                                            <  SRR19973069.238061/147‑1 (MQ=60)
          CCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATTTTCGGCAAGCCGACAATAAACGCGCAGTGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCC                                                                                                                                            <  SRR19973069.2807621/150‑1 (MQ=60)
          CCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCC                                                                                                                                            <  SRR19973069.2807661/150‑1 (MQ=60)
           CGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCG                                                                                                                                           <  SRR19973069.1541887/150‑1 (MQ=60)
           CGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCG                                                                                                                                           <  SRR19973069.1542159/150‑1 (MQ=60)
           CGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCG                                                                                                                                           <  SRR19973069.1541831/150‑1 (MQ=60)
            GCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGG                                                                                                                                          >  SRR19973069.3797442/1‑150 (MQ=60)
              TGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCA                                                                                                                                        >  SRR19973069.1254044/1‑150 (MQ=60)
              TGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCA                                                                                                                                        >  SRR19973069.1253311/1‑150 (MQ=60)
                      AAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATAT                                                                                                                                >  SRR19973069.3801117/1‑150 (MQ=60)
                       AACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATT                                                                                                                               >  SRR19973069.3440811/1‑150 (MQ=60)
                       AACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATT                                                                                                                               <  SRR19973069.3113760/150‑1 (MQ=60)
                       AACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATT                                                                                                                               <  SRR19973069.3113783/150‑1 (MQ=60)
                        ACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTT                                                                                                                              <  SRR19973069.3754670/150‑1 (MQ=60)
                        ACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTT                                                                                                                              <  SRR19973069.3754767/150‑1 (MQ=60)
                          GCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAA                                                                                                                            <  SRR19973069.299502/150‑1 (MQ=60)
                          GCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAA                                                                                                                            <  SRR19973069.299930/150‑1 (MQ=60)
                           CCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAG                                                                                                                           <  SRR19973069.39254/150‑1 (MQ=60)
                            CACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGT                                                                                                                          <  SRR19973069.527104/150‑1 (MQ=60)
                            CACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGT                                                                                                                          <  SRR19973069.137115/150‑1 (MQ=60)
                            CACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGT                                                                                                                          >  SRR19973069.2071755/1‑150 (MQ=60)
                            CACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGT                                                                                                                          >  SRR19973069.2072610/1‑150 (MQ=60)
                                GCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGAT                                                                                                                      <  SRR19973069.2162970/150‑1 (MQ=60)
                                GCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGAT                                                                                                                      <  SRR19973069.2165747/150‑1 (MQ=60)
                                GCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGAT                                                                                                                      <  SRR19973069.2163880/150‑1 (MQ=60)
                                   ATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGC                                                                                                                   >  SRR19973069.2180708/1‑150 (MQ=60)
                                     TTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCC                                                                                                                 <  SRR19973069.1853865/150‑1 (MQ=60)
                                      TTGCCGAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTTATGTTCGGCAAGCCTACAATAAACGCGCATGGTGCAGAATCATTCTCAGAGATATAGGTCTTATAGTCCGGCACCAGATATTTAAGTTGATCGCCCA                                                                                                                <  SRR19973069.2827610/150‑1 (MQ=60)
                                               CGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGA                                                                                                       >  SRR19973069.556436/1‑150 (MQ=60)
                                                GAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCCCCAGATATTTAAGTTGATCGCCCATGGTCGCGAA                                                                                                      >  SRR19973069.1770120/1‑150 (MQ=60)
                                                GAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAA                                                                                                      >  SRR19973069.1770139/1‑150 (MQ=60)
                                                 AAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAT                                                                                                     <  SRR19973069.1655580/150‑1 (MQ=60)
                                                 AAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAT                                                                                                     >  SRR19973069.240919/1‑150 (MQ=60)
                                                 AAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAAT                                                                                                     >  SRR19973069.240243/1‑150 (MQ=60)
                                                  AGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATT                                                                                                    <  SRR19973069.1435418/150‑1 (MQ=60)
                                                   GGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTC                                                                                                   >  SRR19973069.1797907/1‑150 (MQ=60)
                                                   GGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTC                                                                                                   <  SRR19973069.2704837/150‑1 (MQ=60)
                                                    GGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCA                                                                                                  <  SRR19973069.1161475/150‑1 (MQ=60)
                                                    GGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCA                                                                                                  <  SRR19973069.378034/150‑1 (MQ=60)
                                                      AAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATG                                                                                                >  SRR19973069.3400881/1‑150 (MQ=60)
                                                      AAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATG                                                                                                >  SRR19973069.1520932/1‑150 (MQ=60)
                                                          AGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCG                                                                                            >  SRR19973069.2778040/1‑150 (MQ=60)
                                                            CGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGT                                                                                          >  SRR19973069.1854595/1‑150 (MQ=60)
                                                            CGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGT                                                                                          >  SRR19973069.1918605/1‑150 (MQ=60)
                                                             GATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTA                                                                                         >  SRR19973069.2616831/1‑150 (MQ=60)
                                                                 CGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACG                                                                                     <  SRR19973069.3538137/150‑1 (MQ=60)
                                                                  GTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGG                                                                                    <  SRR19973069.2236901/150‑1 (MQ=60)
                                                                     CAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCAC                                                                                 >  SRR19973069.172490/1‑150 (MQ=60)
                                                                     CAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCAC                                                                                 >  SRR19973069.172464/1‑150 (MQ=60)
                                                                      ATATCGGCAATGGCGTGGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCACCGATATAGATCATATCGTCCGTCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACA                                                                                <  SRR19973069.2847705/150‑1 (MQ=60)
                                                                      AGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACA                                                                                >  SRR19973069.3646269/1‑150 (MQ=60)
                                                                           GGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCC                                                                           <  SRR19973069.2834438/150‑1 (MQ=60)
                                                                           GGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCC                                                                           <  SRR19973069.1725351/150‑1 (MQ=60)
                                                                               ATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTCAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAG                                                                       >  SRR19973069.1584204/1‑150 (MQ=60)
                                                                               ATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAG                                                                       <  SRR19973069.1044542/150‑1 (MQ=60)
                                                                               ATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAG                                                                       >  SRR19973069.2402564/1‑150 (MQ=60)
                                                                               ATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAG                                                                       >  SRR19973069.2402598/1‑150 (MQ=60)
                                                                                 CGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCATATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTT                                                                     <  SRR19973069.678026/150‑1 (MQ=60)
                                                                                  GCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTG                                                                    >  SRR19973069.3541682/1‑150 (MQ=60)
                                                                                  GCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAAAGGCACAAATCACCAGTTG                                                                    <  SRR19973069.1318756/150‑1 (MQ=60)
                                                                                  GCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAAAGGCACAAATCACCAGTTG                                                                    <  SRR19973069.3231397/150‑1 (MQ=60)
                                                                                      CGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCT                                                                <  SRR19973069.1379609/150‑1 (MQ=60)
                                                                                      CGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCT                                                                >  SRR19973069.3591439/1‑150 (MQ=60)
                                                                                       GTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCTGTAAACGGCACAAATCCCCAGTTGTGCTG                                                               >  SRR19973069.2588194/1‑150 (MQ=60)
                                                                                       GTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTG                                                               >  SRR19973069.2368432/1‑150 (MQ=60)
                                                                                       GTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTG                                                               >  SRR19973069.3172870/1‑150 (MQ=60)
                                                                                       GTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCCCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTG                                                               >  SRR19973069.2931426/1‑150 (MQ=60)
                                                                                         TGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCC                                                             <  SRR19973069.3299500/150‑1 (MQ=60)
                                                                                         TGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCC                                                             <  SRR19973069.1554804/150‑1 (MQ=60)
                                                                                                 GGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAG                                                     >  SRR19973069.242487/1‑150 (MQ=60)
                                                                                                   CAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCCGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTT                                                   <  SRR19973069.381816/150‑1 (MQ=60)
                                                                                                   CAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTT                                                   <  SRR19973069.2003381/150‑1 (MQ=60)
                                                                                                    AAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTG                                                  >  SRR19973069.3006912/1‑150 (MQ=60)
                                                                                                    AAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTG                                                  <  SRR19973069.281894/150‑1 (MQ=60)
                                                                                                    AAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTG                                                  >  SRR19973069.3006299/1‑150 (MQ=60)
                                                                                                      GCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAA                                                <  SRR19973069.1467236/150‑1 (MQ=60)
                                                                                                            AATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTC                                          >  SRR19973069.38610/1‑150 (MQ=60)
                                                                                                              TAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCAC                                        <  SRR19973069.1543188/150‑1 (MQ=60)
                                                                                                              TAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCAC                                        <  SRR19973069.2489553/150‑1 (MQ=60)
                                                                                                              TAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCAC                                        <  SRR19973069.2592773/150‑1 (MQ=60)
                                                                                                                 ACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCA                                     <  SRR19973069.1037400/150‑1 (MQ=60)
                                                                                                                  CGCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAG                                    <  SRR19973069.1396959/150‑1 (MQ=60)
                                                                                                                   GCGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGG                                   <  SRR19973069.1454818/150‑1 (MQ=60)
                                                                                                                    CGCAGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGA                                  >  SRR19973069.18612/1‑150 (MQ=60)
                                                                                                                      CAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATC                                <  SRR19973069.245843/150‑1 (MQ=60)
                                                                                                                       AGGGTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCT                               <  SRR19973069.1484434/150‑1 (MQ=60)
                                                                                                                          GTGCAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCA                            <  SRR19973069.548929/150‑1 (MQ=60)
                                                                                                                             CAGAATCAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCT                         <  SRR19973069.257168/150‑1 (MQ=60)
                                                                                                                                   CAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGG                   >  SRR19973069.1304712/1‑150 (MQ=60)
                                                                                                                                   CAATCTCAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGG                   >  SRR19973069.1358693/1‑150 (MQ=60)
                                                                                                                                         CAGCGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACC             >  SRR19973069.1107671/1‑150 (MQ=60)
                                                                                                                                            CGATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCT          >  SRR19973069.3814218/1‑150 (MQ=60)
                                                                                                                                              ATATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGA        >  SRR19973069.102939/1‑150 (MQ=60)
                                                                                                                                               TATAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGAC       >  SRR19973069.756789/1‑150 (MQ=60)
                                                                                                                                                 TAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGA     >  SRR19973069.1636658/1‑150 (MQ=60)
                                                                                                                                                 TAGCTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGA     >  SRR19973069.3625711/1‑150 (MQ=60)
                                                                                                                                                    CTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTG  >  SRR19973069.2482947/1‑150 (MQ=60)
                                                                                                                                                    CTCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTG  >  SRR19973069.3239655/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGTTCGCACACCGCTGACTTTCAAACGCCACAGCAATTTTGCCCAGCCGAAGGGAAAGAGCGATCCGTTCAGATCGGCAATCGCCTCGTTGATGTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTG  >  CP009273/3122630‑3122927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑