Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,643,955 C→A 100% A30A (GCC→GCA)  dicB → Qin prophage; cell division inhibition protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,643,9550CA95.1% 791.5 / 26.9 265A30A (GCC→GCA) dicBQin prophage; cell division inhibition protein
Reads supporting (aligned to +/- strand):  ref base C (8/5);  new base A (115/137);  total (123/142)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 265 total aligned reads displayed.

AACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGA  >  CP009273/1643808‑1644100
                                                                                                                                                   |                                                                                                                                                 
 aCGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAAtt                                                                                                                                                >  2:2828558/1‑150 (MQ=255)
   gTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTaa                                                                                                                                              >  1:3894205/1‑150 (MQ=255)
   gTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTAACAGTAACCCAGTATATACTGAAGATGCAATTaa                                                                                                                                              >  1:425287/1‑150 (MQ=255)
      aCCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACaa                                                                                                                                           <  1:533211/150‑1 (MQ=255)
         tGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAagag                                                                                                                                        <  1:3509679/150‑1 (MQ=255)
              gCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAAc                                                                                                                                   <  2:102275/150‑1 (MQ=255)
               cACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACa                                                                                                                                  <  1:1493995/150‑1 (MQ=255)
                 ccAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAg                                                                                                                                <  1:1306391/150‑1 (MQ=255)
                  cAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGa                                                                                                                               <  2:9231/150‑1 (MQ=255)
                   aGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGaa                                                                                                                              >  2:2349891/1‑150 (MQ=255)
                     gCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACg                                                                                                                            <  2:1103764/150‑1 (MQ=255)
                       aCTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACggg                                                                                                                          <  2:2325871/150‑1 (MQ=255)
                        cTGCATCACTAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGTAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTTCTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGa                                                                                                                         <  1:3956699/150‑1 (MQ=255)
                           cATCACAAAATTCATTGTTGAGGACGCTATAATGAAAACGTTAGTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCt                                                                                                                      <  2:4796026/150‑1 (MQ=255)
                            aTCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTa                                                                                                                     <  1:4180036/150‑1 (MQ=255)
                             tCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTAt                                                                                                                    <  2:1334762/150‑1 (MQ=255)
                              cacaAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTAtt                                                                                                                   <  1:1607994/150‑1 (MQ=255)
                               acaAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATta                                                                                                                  <  1:2160845/150‑1 (MQ=255)
                                  aaaTTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATtaaat                                                                                                               <  1:327453/150‑1 (MQ=255)
                                          tgttgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATg                                                                                                       <  2:1983927/150‑1 (MQ=255)
                                          tgttgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATg                                                                                                       >  1:2844010/1‑150 (MQ=255)
                                             tgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCAt                                                                                                    >  1:3361160/1‑150 (MQ=255)
                                               aGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTg                                                                                                  <  1:5110834/150‑1 (MQ=255)
                                                 gACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGtt                                                                                                <  2:162890/150‑1 (MQ=255)
                                                  aCGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGttt                                                                                               >  2:4875240/1‑150 (MQ=255)
                                                   cgcgATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTc                                                                                              >  2:4656271/1‑150 (MQ=255)
                                                      gATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAAt                                                                                           >  2:3932965/1‑150 (MQ=255)
                                                         aaTGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCt                                                                                        >  2:2883779/1‑150 (MQ=255)
                                                         aaTGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCt                                                                                        >  1:1336670/1‑150 (MQ=255)
                                                            gAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGc                                                                                     <  2:4582152/150‑1 (MQ=255)
                                                               aaCGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCg                                                                                  <  1:4699106/150‑1 (MQ=255)
                                                               aaCGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCg                                                                                  >  2:4689910/1‑150 (MQ=255)
                                                                aCGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGt                                                                                 >  2:1824219/1‑150 (MQ=255)
                                                                 cGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGtt                                                                                <  2:3321542/150‑1 (MQ=255)
                                                                   ttattaCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATGGTTTCAATGCTGGCTCGTTTa                                                                              <  2:3022017/150‑1 (MQ=255)
                                                                    tattaCAAAACGTTAATACGTCTGTATGTTGTTTTGAAATTGGTGTCTCTATCAGTAACCAAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGTGAGCTATTAAATAAAATATGCATTTTTTCAATGCTGGCTCGTTTAc                                                                             <  1:140209/150‑1 (MQ=255)
                                                                    tattaCAAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTAc                                                                             <  1:2227184/150‑1 (MQ=255)
                                                                     attaCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACg                                                                            <  2:432918/150‑1 (MQ=255)
                                                                        aCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCt                                                                         >  2:1393235/1‑150 (MQ=255)
                                                                         ccAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTg                                                                        >  2:3528393/1‑150 (MQ=255)
                                                                         ccAAACGGTAATTCGTCTGAAGGTGGGTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACTCGAGAACACCAGACCGGGAGCTATTATTTAAAATTTGCATTGTGTCATTGCTGGCTCGTTGACGTCTg                                                                        >  2:2407546/1‑150 (MQ=255)
                                                                           aaaCGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAt                                                                      <  2:4426472/150‑1 (MQ=255)
                                                                           aaaCGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAt                                                                      <  1:1873861/150‑1 (MQ=255)
                                                                            aaCGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATg                                                                     <  2:1154077/150‑1 (MQ=255)
                                                                            aaCGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATg                                                                     <  1:4730939/150‑1 (MQ=255)
                                                                               gTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCa                                                                  <  1:1231640/150‑1 (MQ=255)
                                                                                   aTACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAg                                                              <  1:4065528/150‑1 (MQ=255)
                                                                                    tACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAgg                                                             >  1:2911573/1‑150 (MQ=255)
                                                                                     aCGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAACAACTGGAGCTATTAAATAAAATATTAATAGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGa                                                            >  2:2676733/1‑150 (MQ=255)
                                                                                      cGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAggtt                                                           <  1:11338/150‑3 (MQ=255)
                                                                                      cGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAt                                                           >  1:4516382/1‑150 (MQ=255)
                                                                                       gTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtg                                                          <  2:2757522/150‑1 (MQ=255)
                                                                                        tCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtgt                                                         <  1:2505736/150‑1 (MQ=255)
                                                                                             aGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGcaca                                                    <  2:493567/150‑1 (MQ=255)
                                                                                               gttgttTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAAt                                                  >  1:4026576/1‑150 (MQ=255)
                                                                                                 tgttTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGa                                                <  1:962642/150‑1 (MQ=255)
                                                                                                 tgttTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGa                                                >  2:85727/1‑150 (MQ=255)
                                                                                                    tttGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAAtt                                             <  1:4256451/150‑1 (MQ=255)
                                                                                                    tttGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAAtt                                             <  2:1701996/150‑1 (MQ=255)
                                                                                                          aTTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCAt                                       <  1:4216639/150‑1 (MQ=255)
                                                                                                            tGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAAAAAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATTCATTGTTTAAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGAAttt                                     >  1:2277887/1‑150 (MQ=255)
                                                                                                               tgtCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTtgtg                                  >  1:3141878/1‑150 (MQ=255)
                                                                                                                gtCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGc                                 <  1:4241353/150‑1 (MQ=255)
                                                                                                                 tCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCt                                <  1:75726/150‑1 (MQ=255)
                                                                                                                   aCTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTg                              <  1:1016344/150‑1 (MQ=255)
                                                                                                                    cTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGt                             <  2:3244644/150‑1 (MQ=255)
                                                                                                                     tATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGtt                            <  2:3515749/150‑1 (MQ=255)
                                                                                                                     tATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGtt                            >  2:5152543/1‑150 (MQ=255)
                                                                                                                     tATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGtt                            >  2:3094784/1‑150 (MQ=255)
                                                                                                                      aTCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTc                           >  1:4579176/1‑150 (MQ=255)
                                                                                                                        cAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTg                         >  1:3438146/1‑150 (MQ=255)
                                                                                                                        cAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTg                         <  2:4207867/150‑1 (MQ=255)
                                                                                                                         aGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGa                        <  2:4122237/150‑1 (MQ=255)
                                                                                                                         aGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGa                        >  2:3128846/1‑150 (MQ=255)
                                                                                                                          gTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGAc                       <  2:1188435/150‑1 (MQ=255)
                                                                                                                           tAACCCAGTATTTACTGAATATGCCATTAACAAGAGAAAACAAGAAAGGGAGCTATTAAATAAAATATGCATTGTTTCACTGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACa                      >  1:2683523/1‑150 (MQ=255)
                                                                                                                           tAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACa                      >  1:3291971/1‑150 (MQ=255)
                                                                                                                           tAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACa                      <  1:1837778/150‑1 (MQ=255)
                                                                                                                            aaCCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAg                     >  1:820295/1‑150 (MQ=255)
                                                                                                                              cccAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGtt                   >  1:2805234/1‑150 (MQ=255)
                                                                                                                              cccAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGtt                   >  1:2779257/1‑150 (MQ=255)
                                                                                                                              cccAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGtt                   <  1:3771489/150‑1 (MQ=255)
                                                                                                                               ccAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGttt                  >  1:3608985/1‑150 (MQ=255)
                                                                                                                                  gTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGACTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTc               >  2:2181712/1‑150 (MQ=255)
                                                                                                                                   tATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCt              >  2:646029/1‑150 (MQ=255)
                                                                                                                                   tATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCt              <  2:1745497/150‑1 (MQ=255)
                                                                                                                                    atatACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTAGTGCTTGTTCTGACAGTTTTTCtt             >  1:4361098/4‑150 (MQ=255)
                                                                                                                                    aTTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCtt             >  1:2610137/1‑150 (MQ=255)
                                                                                                                                         cTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTc        >  1:493748/1‑150 (MQ=255)
                                                                                                                                          tgtaGATGCAATTAAATAGAGTAACCAAGATCGGGAGCTATTAATTTAAATATGCATTTTTTCAATGCTGGCTCGTTTTCGTCTGATGCCAAAAGTATGTGCACAATGAATTCAGCATTTGTGCTTTTTCTTAAATTTTTTCTTGtttga       <  2:10560/147‑3 (MQ=255)
                                                                                                                                          tGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTcc       <  2:3282396/150‑1 (MQ=255)
                                                                                                                                          tGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTcc       >  1:3713498/1‑150 (MQ=255)
                                                                                                                                           gAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCg      >  1:759102/1‑150 (MQ=255)
                                                                                                                                            aaGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCgg     <  1:2337902/150‑1 (MQ=255)
                                                                                                                                            aaGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCgg     >  1:2886023/1‑150 (MQ=255)
                                                                                                                                            aaGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCgg     >  1:3126906/1‑150 (MQ=255)
                                                                                                                                             aGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTGTTGTTTCCGga    <  2:373449/150‑1 (MQ=255)
                                                                                                                                             aGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTGTTGTTTCCGga    <  2:2115026/150‑1 (MQ=255)
                                                                                                                                               aTGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGgaga  >  2:2908033/1‑150 (MQ=255)
                                                                                                                                               aTGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGgaga  <  1:3528393/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGA  >  CP009273/1643808‑1644100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGA  >  CP009273/1643808‑1644100
                                                                                                                                                   |                                                                                                                                                 
AACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAAT                                                                                                                                                 <  SRR19973063.660168/150‑1 (MQ=60)
  CGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTA                                                                                                                                               <  SRR19973063.2552674/150‑1 (MQ=60)
  CGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTA                                                                                                                                               >  SRR19973063.2179109/1‑150 (MQ=60)
  CGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTAGAATTTGGTGTCACTATCAGTAAGCCAGTATTTAGTGATGATGCAATTA                                                                                                                                               >  SRR19973063.4771445/1‑150 (MQ=60)
   GTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAA                                                                                                                                              >  SRR19973063.3906601/1‑150 (MQ=60)
   GTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAA                                                                                                                                              >  SRR19973063.2286173/1‑150 (MQ=60)
   GTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTAACAGTAACCCAGTATATACTGAAGATGCAATTAA                                                                                                                                              >  SRR19973063.426477/1‑150 (MQ=60)
     CACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACA                                                                                                                                            >  SRR19973063.130075/1‑150 (MQ=60)
     CACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGATAATACGTCTGAAGGATGTGTAGAAAAAGGTGTCACTATCAGAAACACAGTAGTAACTGAAGATGCAATTAAAA                                                                                                                                            >  SRR19973063.10603/1‑150 (MQ=60)
      ACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAA                                                                                                                                           <  SRR19973063.3603895/150‑1 (MQ=60)
      ACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAA                                                                                                                                           <  SRR19973063.534716/150‑1 (MQ=60)
      ACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAA                                                                                                                                           <  SRR19973063.534780/150‑1 (MQ=60)
       CCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAG                                                                                                                                          >  SRR19973063.268313/1‑150 (MQ=60)
       CCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAG                                                                                                                                          >  SRR19973063.2867266/1‑150 (MQ=60)
        CTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGA                                                                                                                                         >  SRR19973063.3215538/1‑150 (MQ=60)
         TGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAG                                                                                                                                        <  SRR19973063.3520902/150‑1 (MQ=60)
          GGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGA                                                                                                                                       >  SRR19973063.2836215/1‑150 (MQ=60)
               CACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACA                                                                                                                                  <  SRR19973063.1498574/150‑1 (MQ=60)
                ACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAA                                                                                                                                 <  SRR19973063.4251938/150‑1 (MQ=60)
                ACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAA                                                                                                                                 <  SRR19973063.4251850/150‑1 (MQ=60)
                 CCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAG                                                                                                                                <  SRR19973063.1310280/150‑1 (MQ=60)
                  CAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGA                                                                                                                               <  SRR19973063.1359016/150‑1 (MQ=60)
                  CAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGA                                                                                                                               >  SRR19973063.3292926/1‑150 (MQ=60)
                  CAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGA                                                                                                                               >  SRR19973063.3293064/1‑150 (MQ=60)
                   AGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAA                                                                                                                              >  SRR19973063.374510/1‑150 (MQ=60)
                   AGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAA                                                                                                                              >  SRR19973063.2121646/1‑150 (MQ=60)
                    GGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAAC                                                                                                                             >  SRR19973063.3826596/1‑150 (MQ=60)
                     GCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACG                                                                                                                            <  SRR19973063.121452/150‑1 (MQ=60)
                     GCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACG                                                                                                                            <  SRR19973063.3436520/150‑1 (MQ=60)
                      CACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGG                                                                                                                           >  SRR19973063.3926710/1‑150 (MQ=60)
                        CTGCATCACTAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGTAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTTCTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGA                                                                                                                         <  SRR19973063.3969358/150‑1 (MQ=60)
                          GCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGC                                                                                                                       <  SRR19973063.4800842/150‑1 (MQ=60)
                            ATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTA                                                                                                                     <  SRR19973063.4193387/150‑1 (MQ=60)
                              CACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATT                                                                                                                   <  SRR19973063.1612916/150‑1 (MQ=60)
                               ACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTA                                                                                                                  <  SRR19973063.347724/150‑1 (MQ=60)
                               ACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTA                                                                                                                  <  SRR19973063.2167613/150‑1 (MQ=60)
                               ACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTA                                                                                                                  <  SRR19973063.938649/150‑1 (MQ=60)
                                CAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAA                                                                                                                 <  SRR19973063.2854830/150‑1 (MQ=60)
                                CAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAA                                                                                                                 <  SRR19973063.4263717/150‑1 (MQ=60)
                                  AAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAAT                                                                                                               <  SRR19973063.328403/150‑1 (MQ=60)
                                          TGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATG                                                                                                       >  SRR19973063.2853133/1‑150 (MQ=60)
                                           GTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGC                                                                                                      <  SRR19973063.4123198/150‑1 (MQ=60)
                                             TGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCAT                                                                                                    >  SRR19973063.3371862/1‑150 (MQ=60)
                                             TGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCAT                                                                                                    >  SRR19973063.3560199/1‑150 (MQ=60)
                                               AGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTG                                                                                                  <  SRR19973063.5127277/150‑1 (MQ=60)
                                                GGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGT                                                                                                 <  SRR19973063.2320590/150‑1 (MQ=60)
                                                  ACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGAATTGTTT                                                                                               >  SRR19973063.538410/1‑150 (MQ=60)
                                                      GATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAAT                                                                                           >  SRR19973063.2797350/1‑150 (MQ=60)
                                                         AATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTCTTAAATAAAATATGCATTGTTTCAATGAT                                                                                        >  SRR19973063.1340500/1‑150 (MQ=60)
                                                         AATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCT                                                                                        >  SRR19973063.1340678/1‑150 (MQ=60)
                                                               AACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCG                                                                                  >  SRR19973063.1402257/1‑150 (MQ=60)
                                                               AACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCG                                                                                  <  SRR19973063.4714084/150‑1 (MQ=60)
                                                                 CGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTT                                                                                >  SRR19973063.4564637/1‑150 (MQ=60)
                                                                  GTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTT                                                                               <  SRR19973063.4061972/150‑1 (MQ=60)
                                                                    tagtcCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTAC                                                                             <  SRR19973063.140785/145‑1 (MQ=60)
                                                                    TATTACAAAACGTTAATACGTCTGTATGTTGTTTTGAAATTGGTGTCTCTATCAGTAACCAAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGTGAGCTATTAAATAAAATATGCATTTTTTCAATGCTGGCTCGTTTAC                                                                             <  SRR19973063.140648/150‑1 (MQ=60)
                                                                    TATTACAAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTAC                                                                             <  SRR19973063.2234238/150‑1 (MQ=60)
                                                                         CCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTG                                                                        >  SRR19973063.2752638/1‑150 (MQ=60)
                                                                          CAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGA                                                                       >  SRR19973063.5059908/1‑150 (MQ=60)
                                                                           AAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAT                                                                      <  SRR19973063.1879719/150‑1 (MQ=60)
                                                                            AACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATG                                                                     <  SRR19973063.4746015/150‑1 (MQ=60)
                                                                               GTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCA                                                                  <  SRR19973063.1235318/150‑1 (MQ=60)
                                                                                  AATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAA                                                               <  SRR19973063.2916542/150‑1 (MQ=60)
                                                                                   ATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCACAAG                                                              >  SRR19973063.2111280/1‑150 (MQ=60)
                                                                                   ATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAG                                                              <  SRR19973063.4078561/150‑1 (MQ=60)
                                                                                   ATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAG                                                              <  SRR19973063.1552981/150‑1 (MQ=60)
                                                                                   ATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAG                                                              >  SRR19973063.427825/1‑150 (MQ=60)
                                                                                    TACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGG                                                             <  SRR19973063.2779787/150‑1 (MQ=60)
                                                                                    TACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGG                                                             <  SRR19973063.4256596/150‑1 (MQ=60)
                                                                                    TACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGG                                                             >  SRR19973063.2920862/1‑150 (MQ=60)
                                                                                      CGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGTT                                                           <  SRR19973063.11385/150‑1 (MQ=60)
                                                                                      CGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAT                                                           >  SRR19973063.4530880/1‑150 (MQ=60)
                                                                                      CGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAT                                                           >  SRR19973063.4530952/1‑150 (MQ=60)
                                                                                      CGTCTGAAGGTTGTATTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAT                                                           <  SRR19973063.3658535/150‑1 (MQ=60)
                                                                                        TCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGT                                                         <  SRR19973063.4073096/150‑1 (MQ=60)
                                                                                        TCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGT                                                         <  SRR19973063.2513727/150‑1 (MQ=60)
                                                                                         CTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTG                                                        >  SRR19973063.352526/1‑150 (MQ=60)
                                                                                            AAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCAC                                                     >  SRR19973063.295064/1‑150 (MQ=60)
                                                                                              GGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAA                                                   >  SRR19973063.4734600/1‑150 (MQ=60)
                                                                                               GTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAAT                                                  >  SRR19973063.4039488/1‑150 (MQ=60)
                                                                                                 TGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGA                                                <  SRR19973063.965541/150‑1 (MQ=60)
                                                                                                  GTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAA                                               >  SRR19973063.4276995/1‑150 (MQ=60)
                                                                                                    TTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATT                                             <  SRR19973063.4270001/150‑1 (MQ=60)
                                                                                                     TTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTC                                            <  SRR19973063.4626328/150‑1 (MQ=60)
                                                                                                     TTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTC                                            >  SRR19973063.3714896/1‑150 (MQ=60)
                                                                                                     TTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTC                                            >  SRR19973063.468373/1‑150 (MQ=60)
                                                                                                      TGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCA                                           <  SRR19973063.4230041/150‑1 (MQ=60)
                                                                                                          ATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCAT                                       <  SRR19973063.4230088/150‑1 (MQ=60)
                                                                                                           TTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATT                                      <  SRR19973063.1194781/150‑1 (MQ=60)
                                                                                                           TTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATT                                      >  SRR19973063.1770777/1‑150 (MQ=60)
                                                                                                            TGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTT                                     >  SRR19973063.3465175/1‑150 (MQ=60)
                                                                                                            TGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAAAAAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATTCATTGTTTAAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGAATTT                                     >  SRR19973063.2285159/1‑150 (MQ=60)
                                                                                                               TGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTG                                  >  SRR19973063.1621726/1‑150 (MQ=60)
                                                                                                               TGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTG                                  >  SRR19973063.3151980/1‑150 (MQ=60)
                                                                                                               TGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTG                                  >  SRR19973063.3151713/1‑150 (MQ=60)
                                                                                                               TGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTG                                  >  SRR19973063.4159605/1‑150 (MQ=60)
                                                                                                                GTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGC                                 <  SRR19973063.4254873/150‑1 (MQ=60)
                                                                                                                 TCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCT                                <  SRR19973063.75989/150‑1 (MQ=60)
                                                                                                                 TCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCT                                <  SRR19973063.76011/150‑1 (MQ=60)
                                                                                                                 TCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCT                                <  SRR19973063.76172/150‑1 (MQ=60)
                                                                                                                   ACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTG                              <  SRR19973063.1019409/150‑1 (MQ=60)
                                                                                                                      ATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTC                           >  SRR19973063.4593823/1‑150 (MQ=60)
                                                                                                                       TCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCT                          <  SRR19973063.1330406/150‑1 (MQ=60)
                                                                                                                       TCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCT                          <  SRR19973063.959705/150‑1 (MQ=60)
                                                                                                                        CAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTG                         >  SRR19973063.3449054/1‑150 (MQ=60)
                                                                                                                        CAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTG                         >  SRR19973063.3449079/1‑150 (MQ=60)
                                                                                                                          GTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGAC                       <  SRR19973063.4468538/150‑1 (MQ=60)
                                                                                                                           TAACCCAGTATTTACTGAATATGCCATTAACAAGAGAAAACAAGAAAGGGAGCTATTAAATAAAATATGCATTGTTTCACTGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACA                      >  SRR19973063.2692169/1‑150 (MQ=60)
                                                                                                                           TAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACA                      >  SRR19973063.3302512/1‑150 (MQ=60)
                                                                                                                           TAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACA                      <  SRR19973063.1843531/150‑1 (MQ=60)
                                                                                                                           TAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACA                      <  SRR19973063.1843513/150‑1 (MQ=60)
                                                                                                                           TAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACA                      >  SRR19973063.3367284/1‑150 (MQ=60)
                                                                                                                            AACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAG                     >  SRR19973063.822666/1‑150 (MQ=60)
                                                                                                                             ACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGT                    <  SRR19973063.4034179/150‑1 (MQ=60)
                                                                                                                              CCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTT                   >  SRR19973063.2814252/1‑150 (MQ=60)
                                                                                                                              CCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTT                   >  SRR19973063.2788209/1‑150 (MQ=60)
                                                                                                                              CCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTT                   <  SRR19973063.3783537/150‑1 (MQ=60)
                                                                                                                              CCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTT                   <  SRR19973063.3783578/150‑1 (MQ=60)
                                                                                                                              CCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTT                   <  SRR19973063.1503231/150‑1 (MQ=60)
                                                                                                                              CCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTT                   >  SRR19973063.2380131/1‑150 (MQ=60)
                                                                                                                              CCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTT                   >  SRR19973063.4267518/1‑150 (MQ=60)
                                                                                                                               CCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTT                  >  SRR19973063.3620571/1‑150 (MQ=60)
                                                                                                                                CAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTT                 <  SRR19973063.2837649/150‑1 (MQ=60)
                                                                                                                                 AGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACNATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTT                <  SRR19973063.2688967/150‑1 (MQ=60)
                                                                                                                                 AGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTT                <  SRR19973063.2209054/150‑1 (MQ=60)
                                                                                                                                    ATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTT             >  SRR19973063.2618435/1‑150 (MQ=60)
                                                                                                                                    ATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTT             >  SRR19973063.2240316/1‑150 (MQ=60)
                                                                                                                                    ATATACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTAGTGCTTGTTCTGACAGTTTTTCTT             >  SRR19973063.4375044/1‑150 (MQ=60)
                                                                                                                                     TTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTG            <  SRR19973063.2962892/150‑1 (MQ=60)
                                                                                                                                         CTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTC        >  SRR19973063.495217/1‑150 (MQ=60)
                                                                                                                                         CTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTC        >  SRR19973063.495167/1‑150 (MQ=60)
                                                                                                                                         CTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTC        >  SRR19973063.2507823/1‑150 (MQ=60)
                                                                                                                                          TGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCC       >  SRR19973063.3725372/1‑150 (MQ=60)
                                                                                                                                           GAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCG      >  SRR19973063.761316/1‑150 (MQ=60)
                                                                                                                                           GAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCG      >  SRR19973063.761324/1‑150 (MQ=60)
                                                                                                                                            AAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGG     <  SRR19973063.2345379/150‑1 (MQ=60)
                                                                                                                                            AAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGG     >  SRR19973063.2895243/1‑150 (MQ=60)
                                                                                                                                            AAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGG     >  SRR19973063.3136973/1‑150 (MQ=60)
                                                                                                                                              TATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAG   >  SRR19973063.3254274/1‑150 (MQ=60)
                                                                                                                                              GATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAG   <  SRR19973063.3933476/150‑1 (MQ=60)
                                                                                                                                               ATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGA  <  SRR19973063.3539692/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                 
AACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGA  >  CP009273/1643808‑1644100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑