Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,643,955 C→A 100% A30A (GCC→GCA)  dicB → Qin prophage; cell division inhibition protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,643,9550CA98.3% 747.7 / 1.8 232A30A (GCC→GCA) dicBQin prophage; cell division inhibition protein
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (118/110);  total (121/111)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

CGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAG  >  CP009273/1643810‑1644099
                                                                                                                                                 |                                                                                                                                                
cGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAAt                                                                                                                                                <  1:123624/148‑1 (MQ=255)
cGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAAt                                                                                                                                                <  2:1192562/148‑1 (MQ=255)
  tCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTa                                                                                                                                              <  2:1987917/148‑1 (MQ=255)
   cACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTaa                                                                                                                                             >  1:1669430/1‑148 (MQ=255)
   cACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTaa                                                                                                                                             >  1:622659/1‑148 (MQ=255)
    aCCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAAc                                                                                                                                            >  1:2669227/1‑148 (MQ=255)
    aCCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAAc                                                                                                                                            >  1:70688/1‑148 (MQ=255)
      cTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACaa                                                                                                                                          <  1:3226804/148‑1 (MQ=255)
        ggAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAaga                                                                                                                                        >  2:2888860/1‑148 (MQ=255)
        ggAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAaga                                                                                                                                        >  2:3178148/1‑148 (MQ=255)
        ggAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAaga                                                                                                                                        >  1:1052616/1‑148 (MQ=255)
          aGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAgaga                                                                                                                                      <  1:3061287/148‑1 (MQ=255)
             cACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGaaaa                                                                                                                                   >  2:1113739/1‑148 (MQ=255)
             cACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGaaaa                                                                                                                                   <  2:1642667/148‑1 (MQ=255)
               ccAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACa                                                                                                                                 <  1:3515033/148‑1 (MQ=255)
                  ggCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGACAACAAGa                                                                                                                              >  1:927834/1‑148 (MQ=255)
                  ggCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGa                                                                                                                              <  1:1035333/148‑1 (MQ=255)
                   gCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGaa                                                                                                                             >  1:1877435/1‑148 (MQ=255)
                   gCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGaa                                                                                                                             <  1:515793/148‑1 (MQ=255)
                       tGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACggg                                                                                                                         <  2:846780/148‑1 (MQ=255)
                       tGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACggg                                                                                                                         <  2:341058/148‑1 (MQ=255)
                       tGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACggg                                                                                                                         >  2:237868/1‑148 (MQ=255)
                        gCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGa                                                                                                                        >  2:68976/1‑148 (MQ=255)
                               aaaaTTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATta                                                                                                                 <  1:1725728/148‑1 (MQ=255)
                                aaaTTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATtaa                                                                                                                <  2:1464063/148‑1 (MQ=255)
                                 aaTTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATtaaa                                                                                                               <  2:1959652/148‑1 (MQ=255)
                                    tCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATtaaataa                                                                                                            >  1:2558334/1‑148 (MQ=255)
                                        tgttgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTaaataaaata                                                                                                        >  2:2419767/1‑148 (MQ=255)
                                          ttgATGACGCGTTAATGAAAAAGTTATTACCAAACGTTAATACTTCTGTAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGTGAGCTATTAAATAAAATATg                                                                                                      <  2:2422319/148‑1 (MQ=255)
                                          ttgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATCCGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATg                                                                                                      <  1:1508850/148‑1 (MQ=255)
                                          ttgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATg                                                                                                      <  1:2246463/148‑1 (MQ=255)
                                           tgAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGc                                                                                                     <  2:1864934/148‑1 (MQ=255)
                                            gAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCa                                                                                                    <  1:1313609/148‑1 (MQ=255)
                                             aGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCAt                                                                                                   <  1:589636/148‑1 (MQ=255)
                                              ggACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCAtt                                                                                                  >  1:1325020/1‑148 (MQ=255)
                                                aCGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGt                                                                                                <  1:248163/148‑1 (MQ=255)
                                                aCGCGATAATGAAAACGTTAGTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGt                                                                                                <  2:398119/148‑1 (MQ=255)
                                                    gATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCa                                                                                            <  1:2873588/148‑1 (MQ=255)
                                                     aTAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCaa                                                                                           <  2:2281981/148‑1 (MQ=255)
                                                      tAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAAt                                                                                          >  1:1738747/1‑148 (MQ=255)
                                                       aaTGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATg                                                                                         >  1:1013374/1‑148 (MQ=255)
                                                        aTGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGc                                                                                        <  1:451715/148‑1 (MQ=255)
                                                            aaaCGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTTTTTCAATGCTgga                                                                                    >  2:1826867/1‑147 (MQ=255)
                                                              aCGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTc                                                                                  >  1:2975360/1‑148 (MQ=255)
                                                              aCGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTc                                                                                  >  1:2719132/1‑148 (MQ=255)
                                                              aCGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTc                                                                                  >  2:559764/1‑148 (MQ=255)
                                                                  tattaCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGttt                                                                              >  2:3493257/1‑148 (MQ=255)
                                                                  tattaCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGttt                                                                              >  2:3003586/1‑148 (MQ=255)
                                                                  tattaCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGttt                                                                              >  2:1234073/1‑148 (MQ=255)
                                                                  tattaCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCAAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGttt                                                                              >  1:3375466/1‑148 (MQ=255)
                                                                   attaCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTa                                                                             <  1:2397941/148‑1 (MQ=255)
                                                                   attaCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTa                                                                             >  2:1525499/1‑148 (MQ=255)
                                                                      aCCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGt                                                                          <  1:838198/148‑1 (MQ=255)
                                                                       ccAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTc                                                                         >  2:519150/1‑148 (MQ=255)
                                                                        cAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCt                                                                        >  2:831867/1‑148 (MQ=255)
                                                                         aaaCGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTg                                                                       <  1:1547418/148‑1 (MQ=255)
                                                                             gTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGc                                                                   <  1:982383/148‑1 (MQ=255)
                                                                              ttAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGcc                                                                  >  2:3226296/1‑148 (MQ=255)
                                                                              ttAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGcc                                                                  >  2:1482268/1‑148 (MQ=255)
                                                                               tAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCa                                                                 >  2:20533/1‑148 (MQ=255)
                                                                                  tACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCaaaa                                                              <  2:769077/148‑1 (MQ=255)
                                                                                    cGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAgg                                                            <  1:3289708/148‑1 (MQ=255)
                                                                                      tCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAt                                                          <  2:622716/148‑1 (MQ=255)
                                                                                       cTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtg                                                         >  1:1851594/1‑148 (MQ=255)
                                                                                        tGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtgt                                                        <  1:2868276/148‑1 (MQ=255)
                                                                                         gAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtgtg                                                       >  1:496683/1‑148 (MQ=255)
                                                                                         gAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAtgtg                                                       <  1:3147169/148‑1 (MQ=255)
                                                                                          aaGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGc                                                      <  1:1489104/148‑1 (MQ=255)
                                                                                          aaGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGc                                                      >  2:2692573/1‑148 (MQ=255)
                                                                                             gttgttTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGcaca                                                   <  2:1325020/148‑1 (MQ=255)
                                                                                              ttgttTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACaa                                                  >  1:2756467/1‑148 (MQ=255)
                                                                                               tgttTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAATAGGATGTGCACAAt                                                 >  2:2611477/1‑148 (MQ=255)
                                                                                               tgttTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAAt                                                 <  1:559737/148‑1 (MQ=255)
                                                                                                    tGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAAtt                                            <  1:1470778/148‑1 (MQ=255)
                                                                                                          tGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCAt                                      >  1:1375820/1‑148 (MQ=255)
                                                                                                                cACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAANTAAAATATGCATTGTTTCNATGCTGGCTCGTTTACGTCTGATGCCAAAANGATGTGCACAATGAATTCAGCATTTGTGc                                <  2:2177642/148‑1 (MQ=255)
                                                                                                                cACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGc                                <  1:862708/148‑1 (MQ=255)
                                                                                                                cACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGc                                >  2:2582474/1‑148 (MQ=255)
                                                                                                                cACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGc                                >  1:1356398/1‑148 (MQ=255)
                                                                                                                  cTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCtt                              >  1:624208/1‑148 (MQ=255)
                                                                                                                      cAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTc                          >  2:3061689/1‑148 (MQ=255)
                                                                                                                        gTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTg                        <  1:68980/148‑1 (MQ=255)
                                                                                                                        gTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTg                        <  1:3298252/148‑1 (MQ=255)
                                                                                                                        gTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCAGTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTg                        >  2:1671968/1‑148 (MQ=255)
                                                                                                                         tAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGa                       >  2:565047/1‑148 (MQ=255)
                                                                                                                           aCCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACa                     >  1:1081280/1‑148 (MQ=255)
                                                                                                                           aCCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACa                     <  1:190374/148‑1 (MQ=255)
                                                                                                                           aCCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACa                     >  2:3450616/1‑148 (MQ=255)
                                                                                                                            cccAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAg                    >  2:44129/1‑148 (MQ=255)
                                                                                                                            cccAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAg                    >  1:2946006/1‑148 (MQ=255)
                                                                                                                            cccAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAg                    >  2:2486843/1‑148 (MQ=255)
                                                                                                                               aGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGttt                 <  2:838001/148‑1 (MQ=255)
                                                                                                                                gTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGtttt                >  2:3290869/1‑148 (MQ=255)
                                                                                                                                 tATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGttttt               >  1:33012/1‑148 (MQ=255)
                                                                                                                                      aCTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGt          >  2:1773454/1‑148 (MQ=255)
                                                                                                                                       cTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGtt         <  2:927834/148‑1 (MQ=255)
                                                                                                                                       cTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGtt         <  2:1877435/148‑1 (MQ=255)
                                                                                                                                          aaGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTcc      <  1:2089797/148‑1 (MQ=255)
                                                                                                                                           aGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCg     <  2:3492458/148‑1 (MQ=255)
                                                                                                                                              tGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGgag  >  1:373833/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAG  >  CP009273/1643810‑1644099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCA  >  CP009273/1643807‑1644104
                                                                                                                                                    |                                                                                                                                                     
TAACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAA                                                                                                                                                      >  SRR19973065.639705/1‑150 (MQ=60)
 AACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAAT                                                                                                                                                     <  SRR19973065.564626/150‑1 (MQ=60)
   CGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTA                                                                                                                                                   <  SRR19973065.124613/150‑1 (MQ=60)
   CGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTA                                                                                                                                                   <  SRR19973065.124699/150‑1 (MQ=60)
    GTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAA                                                                                                                                                  >  SRR19973065.626740/1‑150 (MQ=60)
    GTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAA                                                                                                                                                  >  SRR19973065.626682/1‑150 (MQ=60)
    GTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAA                                                                                                                                                  >  SRR19973065.1274358/1‑150 (MQ=60)
    GTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAA                                                                                                                                                  >  SRR19973065.1680597/1‑150 (MQ=60)
     TCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAAC                                                                                                                                                 >  SRR19973065.71428/1‑150 (MQ=60)
     TCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAAC                                                                                                                                                 >  SRR19973065.2685758/1‑150 (MQ=60)
     TCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAAC                                                                                                                                                 <  SRR19973065.409132/150‑1 (MQ=60)
         CTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGA                                                                                                                                             >  SRR19973065.1732025/1‑150 (MQ=60)
         CTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGA                                                                                                                                             >  SRR19973065.1059589/1‑150 (MQ=60)
         CTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGA                                                                                                                                             >  SRR19973065.1920829/1‑150 (MQ=60)
         CTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGA                                                                                                                                             <  SRR19973065.3246176/150‑1 (MQ=60)
             AGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAA                                                                                                                                         >  SRR19973065.47810/1‑150 (MQ=60)
             AGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAA                                                                                                                                         <  SRR19973065.3080003/150‑1 (MQ=60)
                 ACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAA                                                                                                                                     <  SRR19973065.1498433/150‑1 (MQ=60)
                  CCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAG                                                                                                                                    <  SRR19973065.3536341/150‑1 (MQ=60)
                   CAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGACAACAAGA                                                                                                                                   >  SRR19973065.933934/1‑150 (MQ=60)
                   CAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGA                                                                                                                                   >  SRR19973065.933961/1‑150 (MQ=60)
                   CAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGA                                                                                                                                   >  SRR19973065.933931/1‑150 (MQ=60)
                    AGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAA                                                                                                                                  >  SRR19973065.1367908/1‑150 (MQ=60)
                    AGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAA                                                                                                                                  >  SRR19973065.107814/1‑150 (MQ=60)
                    AGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAA                                                                                                                                  >  SRR19973065.1889798/1‑150 (MQ=60)
                    AGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAA                                                                                                                                  >  SRR19973065.1889914/1‑150 (MQ=60)
                     GGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAAC                                                                                                                                 <  SRR19973065.1042186/150‑1 (MQ=60)
                     GGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAAC                                                                                                                                 <  SRR19973065.1042430/150‑1 (MQ=60)
                      GCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACG                                                                                                                                <  SRR19973065.519205/150‑1 (MQ=60)
                         CTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGA                                                                                                                             <  SRR19973065.3247857/150‑1 (MQ=60)
                          TGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAG                                                                                                                            <  SRR19973065.1738557/150‑1 (MQ=60)
                            CATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCT                                                                                                                          >  SRR19973065.3401490/1‑150 (MQ=60)
                               CACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATT                                                                                                                       >  SRR19973065.676252/1‑150 (MQ=60)
                               CACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATT                                                                                                                       >  SRR19973065.308435/1‑150 (MQ=60)
                                  AAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAA                                                                                                                    <  SRR19973065.1737175/150‑1 (MQ=60)
                                     ATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAA                                                                                                                 >  SRR19973065.2807537/1‑150 (MQ=60)
                                     ATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAA                                                                                                                 >  SRR19973065.2574330/1‑150 (MQ=60)
                                          TTGTTGAGGACTCTATAATGAAAACGTTATTACCAAACGTTATTACTTCTGAAGGTTGTTTTGAAATTGGTGTAACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATAT                                                                                                            <  SRR19973065.1349275/150‑1 (MQ=60)
                                             TTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATCCGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCA                                                                                                         <  SRR19973065.1518662/150‑1 (MQ=60)
                                             TTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCA                                                                                                         <  SRR19973065.2260719/150‑1 (MQ=60)
                                               GAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATT                                                                                                       <  SRR19973065.1322296/150‑1 (MQ=60)
                                               GAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATT                                                                                                       >  SRR19973065.1333781/1‑150 (MQ=60)
                                                AGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTG                                                                                                      <  SRR19973065.593293/150‑1 (MQ=60)
                                                   ACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTT                                                                                                   <  SRR19973065.249911/150‑1 (MQ=60)
                                                   ACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTT                                                                                                   <  SRR19973065.2749183/150‑1 (MQ=60)
                                                       GATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAAT                                                                                               <  SRR19973065.2891196/150‑1 (MQ=60)
                                                       GATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAAT                                                                                               <  SRR19973065.482017/150‑1 (MQ=60)
                                                       GATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAAT                                                                                               >  SRR19973065.1750680/1‑150 (MQ=60)
                                                       GATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAAT                                                                                               >  SRR19973065.1750235/1‑150 (MQ=60)
                                                        ATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATG                                                                                              >  SRR19973065.1020089/1‑150 (MQ=60)
                                                        ATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCAAGTCTTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATCCATTGTTTCAATG                                                                                              >  SRR19973065.1020599/1‑150 (MQ=60)
                                                           ATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTG                                                                                           <  SRR19973065.454845/150‑1 (MQ=60)
                                                               AAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTC                                                                                       >  SRR19973065.2993559/1‑150 (MQ=60)
                                                               AAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTC                                                                                       >  SRR19973065.2736044/1‑150 (MQ=60)
                                                                   GTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTT                                                                                   <  SRR19973065.271961/150‑1 (MQ=60)
                                                                   GTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCAAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTT                                                                                   >  SRR19973065.3396109/1‑150 (MQ=60)
                                                                     TATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTAC                                                                                 >  SRR19973065.1536293/1‑150 (MQ=60)
                                                                      ATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACG                                                                                <  SRR19973065.2413020/150‑1 (MQ=60)
                                                                      ATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACG                                                                                <  SRR19973065.2413014/150‑1 (MQ=60)
                                                                         ACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCT                                                                             <  SRR19973065.843660/150‑1 (MQ=60)
                                                                         ACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCT                                                                             <  SRR19973065.843964/150‑1 (MQ=60)
                                                                            AAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAT                                                                          <  SRR19973065.699640/150‑1 (MQ=60)
                                                                            AAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAT                                                                          <  SRR19973065.1938437/150‑1 (MQ=60)
                                                                            AAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAT                                                                          <  SRR19973065.1557655/150‑1 (MQ=60)
                                                                            AAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGAT                                                                          <  SRR19973065.2551831/150‑1 (MQ=60)
                                                                                GTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCA                                                                      <  SRR19973065.988904/150‑1 (MQ=60)
                                                                                   AATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAA                                                                   >  SRR19973065.375186/1‑150 (MQ=60)
                                                                                   AATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAA                                                                   >  SRR19973065.518747/1‑150 (MQ=60)
                                                                                       CGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAT                                                               >  SRR19973065.2606553/1‑150 (MQ=60)
                                                                                       CGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAT                                                               <  SRR19973065.3488136/150‑1 (MQ=60)
                                                                                       CGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAT                                                               <  SRR19973065.2489448/150‑1 (MQ=60)
                                                                                       CGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAT                                                               <  SRR19973065.3309736/150‑1 (MQ=60)
                                                                                       CGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGAT                                                               <  SRR19973065.3309726/150‑1 (MQ=60)
                                                                                        GTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATG                                                              >  SRR19973065.1863899/1‑150 (MQ=60)
                                                                                        GTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATG                                                              >  SRR19973065.1863792/1‑150 (MQ=60)
                                                                                        GTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATG                                                              >  SRR19973065.3010538/1‑150 (MQ=60)
                                                                                         TCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGT                                                             >  SRR19973065.3099057/1‑150 (MQ=60)
                                                                                          CTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTG                                                            >  SRR19973065.500014/1‑150 (MQ=60)
                                                                                           TGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGC                                                           <  SRR19973065.2885870/150‑1 (MQ=60)
                                                                                           TGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGC                                                           <  SRR19973065.2885931/150‑1 (MQ=60)
                                                                                            GAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCA                                                          <  SRR19973065.3166281/150‑1 (MQ=60)
                                                                                            GAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCA                                                          <  SRR19973065.1409344/150‑1 (MQ=60)
                                                                                             AAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCAC                                                         <  SRR19973065.1498720/150‑1 (MQ=60)
                                                                                             AAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCAC                                                         <  SRR19973065.1498349/150‑1 (MQ=60)
                                                                                             AAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCAC                                                         <  SRR19973065.1498642/150‑1 (MQ=60)
                                                                                              AGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACA                                                        >  SRR19973065.1602882/1‑150 (MQ=60)
                                                                                               GGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAA                                                       >  SRR19973065.2773612/1‑150 (MQ=60)
                                                                                                 TTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATG                                                     <  SRR19973065.2172716/150‑1 (MQ=60)
                                                                                                  TGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGA                                                    >  SRR19973065.521687/1‑150 (MQ=60)
                                                                                                  TGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGA                                                    <  SRR19973065.563717/150‑1 (MQ=60)
                                                                                                  TGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGA                                                    <  SRR19973065.563328/150‑1 (MQ=60)
                                                                                                  TGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGA                                                    <  SRR19973065.563301/150‑1 (MQ=60)
                                                                                                       TGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCA                                               <  SRR19973065.1480205/150‑1 (MQ=60)
                                                                                                         AAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGC                                             >  SRR19973065.1152991/1‑150 (MQ=60)
                                                                                                         AAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGC                                             >  SRR19973065.602231/1‑150 (MQ=60)
                                                                                                           ATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCAT                                           >  SRR19973065.1384895/1‑150 (MQ=60)
                                                                                                           ATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCAT                                           >  SRR19973065.1349177/1‑150 (MQ=60)
                                                                                                                TGTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCATCATTTGTG                                      >  SRR19973065.3351493/1‑150 (MQ=60)
                                                                                                                 GTCACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGC                                     >  SRR19973065.1365386/1‑150 (MQ=60)
                                                                                                                   CACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTT                                   >  SRR19973065.628248/1‑150 (MQ=60)
                                                                                                                   CACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTT                                   <  SRR19973065.868115/150‑1 (MQ=60)
                                                                                                                   CACTATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTT                                   <  SRR19973065.868260/150‑1 (MQ=60)
                                                                                                                      TATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTT                                >  SRR19973065.1562479/1‑150 (MQ=60)
                                                                                                                       ATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTC                               >  SRR19973065.2894914/1‑150 (MQ=60)
                                                                                                                       ATCAGTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTC                               >  SRR19973065.2894582/1‑150 (MQ=60)
                                                                                                                           GTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGAC                           <  SRR19973065.3314405/150‑1 (MQ=60)
                                                                                                                           GTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGAC                           <  SRR19973065.69708/150‑1 (MQ=60)
                                                                                                                           GTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGAC                           <  SRR19973065.69712/150‑1 (MQ=60)
                                                                                                                           GTAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGAC                           <  SRR19973065.3318337/150‑1 (MQ=60)
                                                                                                                            TAACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACA                          >  SRR19973065.1088415/1‑150 (MQ=60)
                                                                                                                             AACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAG                         >  SRR19973065.2963919/1‑150 (MQ=60)
                                                                                                                             AACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAG                         >  SRR19973065.2963984/1‑150 (MQ=60)
                                                                                                                             AACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAG                         >  SRR19973065.3320998/1‑150 (MQ=60)
                                                                                                                              ACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGT                        <  SRR19973065.191863/150‑1 (MQ=60)
                                                                                                                              ACCCAGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGT                        <  SRR19973065.1325767/150‑1 (MQ=60)
                                                                                                                                  AGTATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTT                    >  SRR19973065.33423/1‑150 (MQ=60)
                                                                                                                                     ATTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTT                 <  SRR19973065.3212692/150‑1 (MQ=60)
                                                                                                                                      TTTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTG                <  SRR19973065.2688358/150‑1 (MQ=60)
                                                                                                                                       TTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGGGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGT               >  SRR19973065.2434706/1‑150 (MQ=60)
                                                                                                                                       TTACTGAAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTG‑TTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAAGTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTT              <  SRR19973065.712508/150‑1 (MQ=60)
                                                                                                                                             AAGATGCAATTAACAAGAGAAAACTAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCTATGCTTGCTCGTTTACGTCTGATGCCAAAAGGTTGTGCACAATGAATTCAGCATTTGTTCTTGTTCTGACAGTTTTTCTTGTTTCCGG         <  SRR19973065.1838902/150‑1 (MQ=60)
                                                                                                                                             AAGATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGG         <  SRR19973065.2103208/150‑1 (MQ=60)
                                                                                                                                               GATGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAG       >  SRR19973065.376446/1‑150 (MQ=60)
                                                                                                                                                 TGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAG     >  SRR19973065.899552/1‑150 (MQ=60)
                                                                                                                                                 TGCAATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAG     >  SRR19973065.899734/1‑150 (MQ=60)
                                                                                                                                                    AATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCA  <  SRR19973065.3439499/150‑1 (MQ=60)
                                                                                                                                                    AATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCA  >  SRR19973065.1281033/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TAACGTCACCTGGAGGCACCAGGCACTGCATCACAAAATTCATTGTTGAGGACGCGATAATGAAAACGTTATTACCAAACGTTAATACGTCTGAAGGTTGTTTTGAAATTGGTGTCACTATCAGTAACCCAGTATTTACTGAAGATGCCATTAACAAGAGAAAACAAGAACGGGAGCTATTAAATAAAATATGCATTGTTTCAATGCTGGCTCGTTTACGTCTGATGCCAAAAGGATGTGCACAATGAATTCAGCATTTGTGCTTGTTCTGACAGTTTTTCTTGTTTCCGGAGAGCCA  >  CP009273/1643807‑1644104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑