Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,700,008 T→A 5.5% intergenic (+61/‑16) ydgK → / → rsxA DUF2569 family inner membrane protein/SoxR iron‑sulfur cluster reduction factor component; inner membrane protein of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,700,0080TA 5.5% 735.8 / 20.9 231intergenic (+61/‑16)ydgK/rsxADUF2569 family inner membrane protein/SoxR iron‑sulfur cluster reduction factor component; inner membrane protein of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (111/107);  new base A (5/8);  total (116/115)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

TTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAAC  >  CP009273/1699861‑1700157
                                                                                                                                                    |                                                                                                                                                     
tagCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTAt                                                                                                                                                     >  1:885480/3‑150 (MQ=255)
tagCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTAt                                                                                                                                                     <  2:1287231/148‑1 (MQ=255)
 agCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTAtt                                                                                                                                                    >  1:1044710/2‑150 (MQ=255)
 agCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTAtt                                                                                                                                                    >  1:1044662/2‑150 (MQ=255)
 agCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTAtt                                                                                                                                                    >  2:2543298/2‑150 (MQ=255)
  gCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTAttt                                                                                                                                                   <  1:2443352/150‑1 (MQ=255)
   cTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTc                                                                                                                                                  <  2:2440251/150‑1 (MQ=255)
         aCTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCg                                                                                                                                            <  2:454426/150‑1 (MQ=255)
           tttGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGaa                                                                                                                                          <  2:4093056/150‑1 (MQ=255)
                    aaCCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAAtgactg                                                                                                                                 >  1:3342918/1‑150 (MQ=255)
                      ccGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAAtgactgac                                                                                                                               <  1:2922663/150‑1 (MQ=255)
                       cGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATgactgact                                                                                                                              >  1:1604343/1‑150 (MQ=255)
                       cGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATgactgact                                                                                                                              >  1:1754319/1‑150 (MQ=255)
                        gCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAACAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTa                                                                                                                             >  2:2549700/1‑150 (MQ=255)
                            tGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTAATTTCAGGCCGAAAAATGACTGACTACCTg                                                                                                                         >  1:2729230/1‑150 (MQ=255)
                             gATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGt                                                                                                                        >  1:1951553/1‑150 (MQ=255)
                              aTCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGtt                                                                                                                       >  2:1445911/1‑150 (MQ=255)
                                cGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTAc                                                                                                                     <  1:5084909/150‑1 (MQ=255)
                                 gTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACt                                                                                                                    <  1:3751542/150‑1 (MQ=255)
                                      cTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTg                                                                                                               >  1:4735823/1‑150 (MQ=255)
                                         tttCAAACGCTCGTAGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTATGTCg                                                                                                            >  2:3970544/1‑150 (MQ=255)
                                            cAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGaa                                                                                                         >  2:2579012/1‑150 (MQ=255)
                                            cAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGaa                                                                                                         >  1:3489664/1‑150 (MQ=255)
                                                 gCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTa                                                                                                    <  2:4579108/150‑1 (MQ=255)
                                                   tcgtcgCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACt                                                                                                  <  1:4599464/150‑1 (MQ=255)
                                                    cgtcgCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTg                                                                                                 <  2:1023941/150‑1 (MQ=255)
                                                    cgtcgCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTCAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTg                                                                                                 >  2:5026254/1‑150 (MQ=255)
                                                       cgcgGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTAATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTc                                                                                              >  2:2882272/1‑150 (MQ=255)
                                                        gcgGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCa                                                                                             <  2:3527602/150‑1 (MQ=255)
                                                        gcgGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCa                                                                                             <  2:1744787/150‑1 (MQ=255)
                                                         cgGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCaa                                                                                            <  2:2053783/150‑1 (MQ=255)
                                                          gggTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAAt                                                                                           >  2:220570/1‑150 (MQ=255)
                                                           ggTAAAAGCGACGTTTGTTAATCCGTAATAACCTTACAGTTAACCTGTTGTCTCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATa                                                                                          <  1:4467011/150‑1 (MQ=255)
                                                           ggTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATa                                                                                          <  1:1896316/150‑1 (MQ=255)
                                                            gTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATaa                                                                                         <  2:3337431/150‑1 (MQ=255)
                                                                aaGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACttt                                                                                     <  2:4184230/150‑1 (MQ=255)
                                                                  gCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGt                                                                                   <  2:2960744/150‑1 (MQ=255)
                                                                     aCGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACt                                                                                <  1:3914332/150‑1 (MQ=255)
                                                                      cGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTg                                                                               >  1:1123049/1‑150 (MQ=255)
                                                                       gTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTgg                                                                              >  2:4313912/1‑150 (MQ=255)
                                                                       gTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTgg                                                                              >  1:644987/1‑150 (MQ=255)
                                                                       gTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTgg                                                                              <  2:3489431/150‑1 (MQ=255)
                                                                         ttGTGAATCCGTAATAACCTTACAGTTATCCTGTTGTCGCCTGCTCTGGATTAACGGATTATAGGCGGC‑TTTTTTATTTCAGGCCGAATAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTCCTGGTCAATAACTTTGTACTGGTc                                                                            <  1:1833302/150‑1 (MQ=255)
                                                                         ttGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTc                                                                            >  1:1972619/1‑150 (MQ=255)
                                                                           gtgAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCaa                                                                          >  1:4390003/1‑150 (MQ=255)
                                                                           gtgAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCaa                                                                          >  1:3673156/1‑150 (MQ=255)
                                                                            tgAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAg                                                                         >  2:476796/1‑150 (MQ=255)
                                                                             gAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTAATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGt                                                                        >  2:1553568/1‑150 (MQ=255)
                                                                              aaTCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGtt                                                                       <  1:2473569/150‑1 (MQ=255)
                                                                              aaTCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGtt                                                                       <  2:2131450/150‑1 (MQ=255)
                                                                                tCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTtc                                                                     <  1:3880790/150‑1 (MQ=255)
                                                                                  cGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTtctc                                                                   <  1:1203202/150‑1 (MQ=255)
                                                                                   gTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCg                                                                  <  1:2581573/150‑1 (MQ=255)
                                                                                   gTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCg                                                                  >  1:4294107/1‑150 (MQ=255)
                                                                                   gTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTAATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCg                                                                  <  1:370855/150‑1 (MQ=255)
                                                                                   gTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTAATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCg                                                                  <  1:77261/150‑1 (MQ=255)
                                                                                    taataaCCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCgg                                                                 >  2:2767899/1‑150 (MQ=255)
                                                                                       taaCCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGtct                                                              <  1:2072406/150‑1 (MQ=255)
                                                                                         aCCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTctct                                                            <  1:4762924/150‑1 (MQ=255)
                                                                                         aCCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTctct                                                            <  2:811914/150‑1 (MQ=255)
                                                                                         aCCTTACAGTTAACCTGTTGTCGCCTGATCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGTCTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTctct                                                            <  2:4921103/150‑1 (MQ=255)
                                                                                          ccTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTg                                                           >  2:3683893/1‑150 (MQ=255)
                                                                                            ttACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTCCTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTc                                                         >  2:342046/1‑150 (MQ=255)
                                                                                             tACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTcc                                                        <  2:1164917/150‑1 (MQ=255)
                                                                                               cAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGt                                                      <  2:4377501/150‑1 (MQ=255)
                                                                                                aGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGtt                                                     >  1:2050100/1‑150 (MQ=255)
                                                                                                 gTTAACCTGTTGTCGCCTGCTCTGGATGAACGGATAATAGGCGGC‑TTTTTTAGTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGttt                                                    <  2:3105930/150‑1 (MQ=255)
                                                                                                 gTTAACCTGTTGTCGCCTGATCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGttt                                                    <  2:2665135/150‑1 (MQ=255)
                                                                                                  ttAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTa                                                   >  1:1464343/1‑150 (MQ=255)
                                                                                                  ttAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTa                                                   <  1:3573755/150‑1 (MQ=255)
                                                                                                   tAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTTTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTAt                                                  >  1:382975/1‑150 (MQ=255)
                                                                                                   tAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTAt                                                  >  1:2971839/1‑150 (MQ=255)
                                                                                                    aaCCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATg                                                 >  1:3082562/1‑150 (MQ=255)
                                                                                                    aaCCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTGATg                                                 >  1:3617701/1‑150 (MQ=255)
                                                                                                      ccTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATggg                                               >  1:1798019/1‑150 (MQ=255)
                                                                                                      ccTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATggg                                               >  1:1798732/1‑150 (MQ=255)
                                                                                                        tgttgtAGCCAGCTATTGATTAACGGATTATTTGCGGCTTTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATgggg                                              <  2:3574906/150‑1 (MQ=255)
                                                                                                           tgtCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGttt                                          <  2:3432039/150‑1 (MQ=255)
                                                                                                           tgtCGCCTGCTCTGGATGAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGttt                                          <  2:2536739/150‑1 (MQ=255)
                                                                                                              cGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACAGTTTGTCGGAACTGTACTGGTCAATAACTTTTTACTGGTCAAGTTTCTCGGTCTCTGTCCGTGTATGGGGGTTTCCa                                       >  1:4452196/1‑150 (MQ=255)
                                                                                                                ccTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCaaa                                     >  2:3797141/1‑150 (MQ=255)
                                                                                                                      ctGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTg                               <  1:3612045/150‑1 (MQ=255)
                                                                                                                      ctGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTg                               >  1:1068525/1‑150 (MQ=255)
                                                                                                                         gATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGaa                            <  2:1644670/150‑1 (MQ=255)
                                                                                                                            tAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAAcc                         <  1:4103324/150‑1 (MQ=255)
                                                                                                                            tAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAAcc                         >  1:3169543/1‑150 (MQ=255)
                                                                                                                              aCGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACcgc                       >  2:5090449/1‑150 (MQ=255)
                                                                                                                              aCGGATAATAGGCGGC‑TTTTGAATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACcgc                       <  2:880904/150‑1 (MQ=255)
                                                                                                                                  ataataGGCGGC‑TTTTTAATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATg                   <  2:4867209/150‑1 (MQ=255)
                                                                                                                                     attggcggc‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTAAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGc                <  1:4938142/147‑1 (MQ=255)
                                                                                                                                      taGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTTGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCa               >  2:456642/1‑150 (MQ=255)
                                                                                                                                       aGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGGTACTGTTGGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCAt              <  2:1898922/150‑1 (MQ=255)
                                                                                                                                         gcggc‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATgg            <  1:2543298/150‑1 (MQ=255)
                                                                                                                                          cggc‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATggg           >  2:119010/1‑150 (MQ=255)
                                                                                                                                          cggc‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATggg           >  1:4831075/1‑150 (MQ=255)
                                                                                                                                            gc‑TTTTTAATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGc         >  2:1475922/1‑150 (MQ=255)
                                                                                                                                                tttttATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTgg      >  1:2102774/1‑150 (MQ=255)
                                                                                                                                                  tttATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGca    >  1:2609995/1‑150 (MQ=255)
                                                                                                                                                   ttATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGACCTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGcaa   <  1:1445816/150‑1 (MQ=255)
                                                                                                                                                    tATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCTGAACTGTACTGGTCAATAACTTTGTACTGTTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGcaac  <  2:3400143/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGC‑TTTTTTATTTCAGGCCGAAAAATGACTGACTACCTGTTACTGTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAGTTTCTCGGTCTCTGTCCGTTTATGGGGGTTTCCAAAAAACTGGAAACCGCGATGGGCATGGGGCTGGCAAC  >  CP009273/1699861‑1700157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A