Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,955,401 C→A 79.5% S361S (TCC→TCA)  argS → arginyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,955,4010CA79.5% 555.4 / 181.4 298S361S (TCC→TCA) argSarginyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (29/32);  new base A (105/132);  total (134/164)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

CTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG  >  CP009273/1955254‑1955548
                                                                                                                                                   |                                                                                                                                                   
ctACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGt                                                                                                                                                   >  2:3185800/1‑150 (MQ=255)
 tACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTa                                                                                                                                                  >  2:2372857/1‑150 (MQ=255)
  acacCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTAc                                                                                                                                                 >  2:3306215/1‑150 (MQ=255)
   caccaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTAcc                                                                                                                                                <  1:163937/150‑1 (MQ=255)
       accacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTg                                                                                                                                            <  1:4298994/150‑1 (MQ=255)
        ccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTgg                                                                                                                                           <  2:1294820/150‑1 (MQ=255)
         cacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGa                                                                                                                                          >  1:2733813/1‑150 (MQ=255)
         cacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGa                                                                                                                                          <  1:1484394/150‑1 (MQ=255)
            tGATATCGCCTTTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAaca                                                                                                                                       <  2:4696108/150‑1 (MQ=255)
            tGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAaca                                                                                                                                       <  1:786006/150‑1 (MQ=255)
            tGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAaca                                                                                                                                       <  2:145830/150‑1 (MQ=255)
             gATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAacac                                                                                                                                      <  2:1856653/150‑1 (MQ=255)
             gATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAacac                                                                                                                                      >  1:4432202/1‑150 (MQ=255)
                atCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAAcaccac                                                                                                                                   >  2:2216462/1‑150 (MQ=255)
                  cGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACAt                                                                                                                                 >  1:556824/1‑150 (MQ=255)
                     cTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGtt                                                                                                                              >  2:4049958/1‑150 (MQ=255)
                           gAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCat                                                                                                                        >  2:955021/1‑150 (MQ=255)
                           gAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCat                                                                                                                        >  1:4707871/1‑150 (MQ=255)
                               tatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                    >  2:5131623/1‑150 (MQ=255)
                               tatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                    <  2:2675372/150‑1 (MQ=255)
                                atCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGc                                                                                                                   <  2:2424430/150‑1 (MQ=255)
                                  cGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTg                                                                                                                 <  1:4331461/150‑1 (MQ=255)
                                   gTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTgg                                                                                                                <  2:4976914/150‑1 (MQ=255)
                                     tATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGt                                                                                                              <  2:1652100/150‑1 (MQ=255)
                                     tATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGt                                                                                                              <  2:531698/150‑1 (MQ=255)
                                       tGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaa                                                                                                            <  2:3685027/150‑1 (MQ=255)
                                       tGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaa                                                                                                            <  2:3769295/150‑1 (MQ=255)
                                         aaaCACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCCTGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGACCACCACATGTTCGGCATGATGCTGGGTAAAg                                                                                                          >  1:5150379/1‑150 (MQ=255)
                                         aaaCACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAg                                                                                                          <  1:1346735/150‑1 (MQ=255)
                                          aaCACTGCCAGCCGATCGCTTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGa                                                                                                         <  2:2642700/150‑1 (MQ=255)
                                            cacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACg                                                                                                       <  1:2156207/150‑1 (MQ=255)
                                                 cATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCaaa                                                                                                  >  2:1627333/1‑150 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                >  1:1076924/1‑150 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                <  1:187377/150‑1 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                <  2:299443/150‑1 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                <  2:2255528/150‑1 (MQ=255)
                                                   tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                                >  1:3657354/1‑150 (MQ=255)
                                                    gCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcca                                                                                               <  1:4917409/150‑2 (MQ=255)
                                                       gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTc                                                                                            >  1:3965460/1‑150 (MQ=255)
                                                       gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTc                                                                                            <  1:3568749/150‑1 (MQ=255)
                                                        aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGACTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAATGACGGCCAACCGTTCa                                                                                           >  1:3374434/1‑150 (MQ=255)
                                                        aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCa                                                                                           <  2:2821703/150‑1 (MQ=255)
                                                          cgcgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCaaa                                                                                         >  2:570510/1‑150 (MQ=255)
                                                           gcgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCaaaa                                                                                        >  2:3984680/1‑150 (MQ=255)
                                                            cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAAc                                                                                       <  2:4363004/150‑1 (MQ=255)
                                                             gTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGGTCAAAAcc                                                                                      >  2:4746659/1‑150 (MQ=255)
                                                               gCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCcg                                                                                    <  2:4174932/150‑1 (MQ=255)
                                                                cTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGTAATCAGTACAGCTGGAACACCACATGTTCGGCATGATTCTGGGTAAAGACGGCAAACCGTTCAAAACCcgc                                                                                   <  2:1842244/150‑1 (MQ=255)
                                                                  gTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCcgcgc                                                                                 >  1:3357227/1‑150 (MQ=255)
                                                                   tATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCACACCGTTCAAAACCcgcgcg                                                                                >  2:4459048/1‑150 (MQ=255)
                                                                     ttACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCggg                                                                              >  1:3069680/1‑150 (MQ=255)
                                                                        cATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGggtgg                                                                           >  2:4297613/1‑150 (MQ=255)
                                                                        cATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGggtgg                                                                           <  2:792812/150‑1 (MQ=255)
                                                                         aTCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGggtggt                                                                          <  2:151371/150‑1 (MQ=255)
                                                                            gACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACa                                                                       <  1:2326975/150‑1 (MQ=255)
                                                                            gACTCCCGTCAGCATCAACACCTGATGCAGGCAGGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACa                                                                       <  2:179973/150‑1 (MQ=255)
                                                                              cTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGt                                                                     <  1:2811432/150‑1 (MQ=255)
                                                                               tCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTg                                                                    >  2:3449302/1‑150 (MQ=255)
                                                                                cccGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGa                                                                   >  1:3748881/1‑150 (MQ=255)
                                                                                accGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGa                                                                   >  1:2764902/2‑150 (MQ=255)
                                                                                  cGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGaaa                                                                 >  2:2715438/1‑150 (MQ=255)
                                                                                    tCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACt                                                               <  2:2110444/150‑1 (MQ=255)
                                                                                       gCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGc                                                            >  2:4875861/1‑150 (MQ=255)
                                                                                             acacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATct                                                      <  2:3499929/150‑1 (MQ=255)
                                                                                              cacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctg                                                     <  2:1914378/150‑1 (MQ=255)
                                                                                              cacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctg                                                     <  1:4484916/150‑1 (MQ=255)
                                                                                                cccgATGCCCGCAGGGGCGCTCGTCCGGACCGCAGGCTCTGTCCCGGCATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCACACCGTTCAAACCCCGCGCGGGTGGTACAGTGCAACGGGCCGATctgct                                                   <  2:5085416/147‑1 (MQ=255)
                                                                                                 cTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctgctg                                                  >  1:3477874/1‑150 (MQ=255)
                                                                                                  tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTgg                                                 <  1:171613/150‑1 (MQ=255)
                                                                                                  tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTgg                                                 >  2:4087505/1‑150 (MQ=255)
                                                                                                    aTGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGAt                                               >  1:4611303/1‑150 (MQ=255)
                                                                                                       cAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGaa                                            <  2:4549708/150‑1 (MQ=255)
                                                                                                          gCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGcc                                         >  1:2921563/1‑150 (MQ=255)
                                                                                                            aTGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCAGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAACCCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGATGCCCt                                       <  2:7593/150‑1 (MQ=255)
                                                                                                             tGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTg                                      <  2:1987588/150‑1 (MQ=255)
                                                                                                               ggCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGa                                    >  1:1189985/1‑150 (MQ=255)
                                                                                                                gCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGaa                                   <  1:1530860/150‑1 (MQ=255)
                                                                                                                 ctaTCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCAAGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAc                                  >  1:5078511/3‑150 (MQ=255)
                                                                                                                 cGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAc                                  >  1:2524088/1‑150 (MQ=255)
                                                                                                                   aTCGTCCGTAAAGCAGGCTATGAACCGGACTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACACGCGCGGGAGGTACAGTGACACTGGCCGATCTGCTGGATGACGCCCTGGAACGt                                >  2:1779103/1‑150 (MQ=255)
                                                                                                                     cGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGc                              >  1:730650/1‑150 (MQ=255)
                                                                                                                         cGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGc                          <  1:1970884/150‑1 (MQ=255)
                                                                                                                            aaaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGt                       >  1:4111805/1‑150 (MQ=255)
                                                                                                                            aaaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGt                       <  2:3257548/150‑1 (MQ=255)
                                                                                                                             aaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                      <  2:2336892/150‑1 (MQ=255)
                                                                                                                             aaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                      <  1:1052270/150‑1 (MQ=255)
                                                                                                                             aaGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                      <  2:1256708/150‑1 (MQ=255)
                                                                                                                              aGCTGGCTATGTACCGGACTCCGTACCGCTGGAACACCACAGGTTCGTCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCt                     <  2:876271/150‑1 (MQ=255)
                                                                                                                              aGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCt                     <  1:4814931/150‑1 (MQ=255)
                                                                                                                              aGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCt                     <  2:4262193/150‑1 (MQ=255)
                                                                                                                               gCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtg                    <  1:1593984/150‑1 (MQ=255)
                                                                                                                                   gCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtggtgg                <  1:1222310/150‑1 (MQ=255)
                                                                                                                                     tATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCa              <  1:3917081/150‑1 (MQ=255)
                                                                                                                                      aTGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAg             >  2:4246547/1‑150 (MQ=255)
                                                                                                                                        gTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGACCGTGCACGCCGTCTGGTGGCAGaa           >  1:3201743/1‑150 (MQ=255)
                                                                                                                                              gAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAATACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAc     >  1:4955724/1‑150 (MQ=255)
                                                                                                                                               aaTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAcc    >  2:4608344/1‑150 (MQ=255)
                                                                                                                                                aTCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAccc   <  2:1060880/150‑1 (MQ=255)
                                                                                                                                                aTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAccc   <  1:2684831/150‑1 (MQ=255)
                                                                                                                                                aTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAccc   <  2:938930/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG  >  CP009273/1955254‑1955548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGG  >  CP009273/1955253‑1955549
                                                                                                                                                    |                                                                                                                                                    
TCTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAG                                                                                                                                                     <  SRR19973063.1941215/150‑1 (MQ=60)
  TACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTA                                                                                                                                                   >  SRR19973063.1461033/1‑150 (MQ=60)
    CACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACC                                                                                                                                                 <  SRR19973063.2701405/150‑1 (MQ=60)
    CACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACC                                                                                                                                                 <  SRR19973063.2701425/150‑1 (MQ=60)
    CACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACC                                                                                                                                                 <  SRR19973063.2679450/150‑1 (MQ=60)
    CACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACC                                                                                                                                                 <  SRR19973063.164442/150‑1 (MQ=60)
     ACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCG                                                                                                                                                >  SRR19973063.2789889/1‑150 (MQ=60)
     ACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCG                                                                                                                                                >  SRR19973063.489950/1‑150 (MQ=60)
      CCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGC                                                                                                                                               >  SRR19973063.865068/1‑150 (MQ=60)
      CCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGC                                                                                                                                               >  SRR19973063.4947085/1‑150 (MQ=60)
       CACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCT                                                                                                                                              >  SRR19973063.4990832/1‑150 (MQ=60)
        ACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTG                                                                                                                                             <  SRR19973063.4312654/150‑1 (MQ=60)
          CACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGA                                                                                                                                           <  SRR19973063.4369703/150‑1 (MQ=60)
          CACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGA                                                                                                                                           <  SRR19973063.1488937/150‑1 (MQ=60)
          CACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGA                                                                                                                                           >  SRR19973063.2742637/1‑150 (MQ=60)
           ACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAA                                                                                                                                          >  SRR19973063.1569026/1‑150 (MQ=60)
             TGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACA                                                                                                                                        >  SRR19973063.763728/1‑150 (MQ=60)
             TGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACA                                                                                                                                        <  SRR19973063.788283/150‑1 (MQ=60)
              GATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACAC                                                                                                                                       >  SRR19973063.4446424/1‑150 (MQ=60)
                   CGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACAT                                                                                                                                  >  SRR19973063.2349680/1‑150 (MQ=60)
                   CGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACAT                                                                                                                                  >  SRR19973063.558454/1‑150 (MQ=60)
                   CGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACAT                                                                                                                                  >  SRR19973063.558472/1‑150 (MQ=60)
                      CTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTT                                                                                                                               <  SRR19973063.1191439/150‑1 (MQ=60)
                      CTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTT                                                                                                                               >  SRR19973063.1514692/1‑150 (MQ=60)
                       TGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTC                                                                                                                              >  SRR19973063.4259948/1‑150 (MQ=60)
                          GCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGC                                                                                                                           <  SRR19973063.3968776/150‑1 (MQ=60)
                            GAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCAT                                                                                                                         >  SRR19973063.4722869/1‑150 (MQ=60)
                             AAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATG                                                                                                                        >  SRR19973063.1070759/1‑150 (MQ=60)
                                 ATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGC                                                                                                                    <  SRR19973063.2181448/150‑1 (MQ=60)
                                   CGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTG                                                                                                                  <  SRR19973063.4345263/150‑1 (MQ=60)
                                    GTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGG                                                                                                                 <  SRR19973063.1643101/150‑1 (MQ=60)
                                          AAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCCTGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGACCACCACATGTTCGGCATGATGCTGGGTAAAG                                                                                                           >  SRR19973063.5166954/1‑150 (MQ=60)
                                          AAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAG                                                                                                           <  SRR19973063.1350779/150‑1 (MQ=60)
                                          AAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAG                                                                                                           <  SRR19973063.1621830/150‑1 (MQ=60)
                                             CACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACG                                                                                                        >  SRR19973063.3268002/1‑150 (MQ=60)
                                             CACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACG                                                                                                        <  SRR19973063.2162964/150‑1 (MQ=60)
                                              ACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGG                                                                                                       <  SRR19973063.3006494/150‑1 (MQ=60)
                                                TGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCA                                                                                                     >  SRR19973063.4028975/1‑150 (MQ=60)
                                                AGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTACGGCATGATGCTGGGTAAAGACGGCA                                                                                                     >  SRR19973063.3569477/1‑150 (MQ=60)
                                                 GCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAA                                                                                                    <  SRR19973063.1106011/150‑1 (MQ=60)
                                                  CATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAA                                                                                                   >  SRR19973063.4341716/1‑150 (MQ=60)
                                                   ATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAC                                                                                                  >  SRR19973063.4448319/1‑150 (MQ=60)
                                                   ATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAC                                                                                                  >  SRR19973063.4546411/1‑150 (MQ=60)
                                                    TGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACC                                                                                                 >  SRR19973063.3669081/1‑150 (MQ=60)
                                                    TGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACC                                                                                                 <  SRR19973063.187954/150‑1 (MQ=60)
                                                    TGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACC                                                                                                 >  SRR19973063.1080193/1‑150 (MQ=60)
                                                     GCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCA                                                                                                <  SRR19973063.4933287/150‑1 (MQ=60)
                                                        GATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTC                                                                                             >  SRR19973063.4320897/1‑150 (MQ=60)
                                                        GATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCGCATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTC                                                                                             <  SRR19973063.3404866/150‑1 (MQ=60)
                                                        GATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTC                                                                                             <  SRR19973063.3580190/150‑1 (MQ=60)
                                                        GATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTC                                                                                             >  SRR19973063.3978149/1‑150 (MQ=60)
                                                         ATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGACTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAATGACGGCCAACCGTTCA                                                                                            >  SRR19973063.3385183/1‑150 (MQ=60)
                                                             CGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAAC                                                                                        >  SRR19973063.52280/1‑150 (MQ=60)
                                                               TGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCC                                                                                      <  SRR19973063.4763000/150‑1 (MQ=60)
                                                               TGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCC                                                                                      <  SRR19973063.3589088/150‑1 (MQ=60)
                                                                 CTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGC                                                                                    >  SRR19973063.1278622/1‑150 (MQ=60)
                                                                  TGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCG                                                                                   <  SRR19973063.512748/150‑1 (MQ=60)
                                                                   GTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGC                                                                                  >  SRR19973063.2834638/1‑150 (MQ=60)
                                                                   GTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGC                                                                                  >  SRR19973063.3367914/1‑150 (MQ=60)
                                                                    TATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCG                                                                                 >  SRR19973063.4659792/1‑150 (MQ=60)
                                                                      TTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGG                                                                               >  SRR19973063.3079538/1‑150 (MQ=60)
                                                                      TTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGG                                                                               >  SRR19973063.4481218/1‑150 (MQ=60)
                                                                       TACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGT                                                                              >  SRR19973063.172056/1‑150 (MQ=60)
                                                                       TACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGT                                                                              <  SRR19973063.1210636/150‑1 (MQ=60)
                                                                         CATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGG                                                                            >  SRR19973063.4390172/1‑150 (MQ=60)
                                                                         CATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGG                                                                            <  SRR19973063.2994845/150‑1 (MQ=60)
                                                                          ATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGT                                                                           >  SRR19973063.1495260/1‑150 (MQ=60)
                                                                             GACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACA                                                                        <  SRR19973063.2334425/150‑1 (MQ=60)
                                                                              ACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAG                                                                       <  SRR19973063.3917638/150‑1 (MQ=60)
                                                                              ACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAG                                                                       <  SRR19973063.3917685/150‑1 (MQ=60)
                                                                               CTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGT                                                                      <  SRR19973063.2820468/150‑1 (MQ=60)
                                                                               CTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGT                                                                      <  SRR19973063.3510816/150‑1 (MQ=60)
                                                                                 CCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGA                                                                    >  SRR19973063.3760859/1‑150 (MQ=60)
                                                                                 CCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGA                                                                    >  SRR19973063.2773921/1‑150 (MQ=60)
                                                                                 CCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGA                                                                    >  SRR19973063.2925092/1‑150 (MQ=60)
                                                                                 ACCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGA                                                                    >  SRR19973063.2773816/1‑150 (MQ=60)
                                                                                   CGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAA                                                                  >  SRR19973063.1963840/1‑150 (MQ=60)
                                                                                    GTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAAC                                                                 >  SRR19973063.2426681/1‑150 (MQ=60)
                                                                                      CAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTG                                                               >  SRR19973063.616022/1‑150 (MQ=60)
                                                                                       AGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGG                                                              >  SRR19973063.5009831/1‑150 (MQ=60)
                                                                                        GCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGC                                                             <  SRR19973063.1994093/150‑1 (MQ=60)
                                                                                         CATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCC                                                            >  SRR19973063.2755752/1‑150 (MQ=60)
                                                                                         CATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCC                                                            >  SRR19973063.2755788/1‑150 (MQ=60)
                                                                                          ATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCG                                                           >  SRR19973063.1704119/1‑150 (MQ=60)
                                                                                            CAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGAT                                                         >  SRR19973063.3482198/1‑150 (MQ=60)
                                                                                            CAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGAT                                                         <  SRR19973063.1833217/150‑1 (MQ=60)
                                                                                               CACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTG                                                      <  SRR19973063.4499300/150‑1 (MQ=60)
                                                                                               CACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTG                                                      <  SRR19973063.1916111/150‑1 (MQ=60)
                                                                                                  CTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTG                                                   >  SRR19973063.3488965/1‑150 (MQ=60)
                                                                                                   TGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGG                                                  <  SRR19973063.172143/150‑1 (MQ=60)
                                                                                                     ATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGAT                                                <  SRR19973063.4666834/150‑1 (MQ=60)
                                                                                                     ATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGAT                                                >  SRR19973063.4626049/1‑150 (MQ=60)
                                                                                                     ATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGAT                                                >  SRR19973063.4626023/1‑150 (MQ=60)
                                                                                                     ATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGAT                                                >  SRR19973063.37387/1‑150 (MQ=60)
                                                                                                      TGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATG                                               >  SRR19973063.2310750/1‑150 (MQ=60)
                                                                                                         AGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAG                                            <  SRR19973063.408841/150‑1 (MQ=60)
                                                                                                           GCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCC                                          >  SRR19973063.2930874/1‑150 (MQ=60)
                                                                                                             ATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCT                                        <  SRR19973063.3661355/150‑1 (MQ=60)
                                                                                                             ATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCT                                        <  SRR19973063.1547337/150‑1 (MQ=60)
                                                                                                             ATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCT                                        <  SRR19973063.1790035/150‑1 (MQ=60)
                                                                                                              TGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTG                                       >  SRR19973063.1483366/1‑150 (MQ=60)
                                                                                                                GGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGA                                     >  SRR19973063.1193562/1‑150 (MQ=60)
                                                                                                                 GCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAA                                    <  SRR19973063.1535556/150‑1 (MQ=60)
                                                                                                                  CTATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCAAGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAC                                   >  SRR19973063.5094846/1‑150 (MQ=60)
                                                                                                                  CGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAC                                   >  SRR19973063.2532139/1‑150 (MQ=60)
                                                                                                                  CGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAC                                   <  SRR19973063.3316789/150‑1 (MQ=60)
                                                                                                                      CGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGC                               >  SRR19973063.732795/1‑150 (MQ=60)
                                                                                                                         CCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACG                            <  SRR19973063.3974046/150‑1 (MQ=60)
                                                                                                                         CCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACG                            >  SRR19973063.651337/1‑150 (MQ=60)
                                                                                                                          CGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGC                           <  SRR19973063.1977048/150‑1 (MQ=60)
                                                                                                                           GTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCC                          >  SRR19973063.758613/1‑150 (MQ=60)
                                                                                                                           GTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCC                          <  SRR19973063.1209501/150‑1 (MQ=60)
                                                                                                                           GTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCC                          >  SRR19973063.4908220/1‑150 (MQ=60)
                                                                                                                             AAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGT                        <  SRR19973063.3198393/150‑1 (MQ=60)
                                                                                                                             AAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGT                        >  SRR19973063.4124975/1‑150 (MQ=60)
                                                                                                                              AAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTC                       <  SRR19973063.1055457/150‑1 (MQ=60)
                                                                                                                               AGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCT                      <  SRR19973063.4830338/150‑1 (MQ=60)
                                                                                                                               AGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCT                      >  SRR19973063.4942748/1‑150 (MQ=60)
                                                                                                                                GCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTG                     <  SRR19973063.1598876/150‑1 (MQ=60)
                                                                                                                                  AGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGT                   <  SRR19973063.5153525/150‑1 (MQ=60)
                                                                                                                                    GCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGG                 <  SRR19973063.1225969/150‑1 (MQ=60)
                                                                                                                                      TATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCA               <  SRR19973063.3196045/150‑1 (MQ=60)
                                                                                                                                      TATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCA               <  SRR19973063.3929580/150‑1 (MQ=60)
                                                                                                                                      TATGTACCGGAATCAGAACCGCTGGAACACCAAATGTTCGGCATGATGCTGGGAAAAGACGGAAAACCGTTCAAAACACGCGCGGGTGGAACAGTGAAACTGGCCGATATGAAGGATGAAGACCTGGAACTTGCACGCCGTCTGGTGGCA               >  SRR19973063.4430375/1‑150 (MQ=60)
                                                                                                                                       ATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATTCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAG              <  SRR19973063.3714089/150‑1 (MQ=60)
                                                                                                                                        TGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGA             <  SRR19973063.2061356/150‑1 (MQ=60)
                                                                                                                                         GTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGACCGTGCACGCCGTCTGGTGGCAGAA            >  SRR19973063.3212038/1‑150 (MQ=60)
                                                                                                                                             CGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGA        >  SRR19973063.1294086/1‑150 (MQ=60)
                                                                                                                                               GAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAATACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAC      >  SRR19973063.4971745/1‑150 (MQ=60)
                                                                                                                                                AATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCATTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACC     <  SRR19973063.4152678/150‑1 (MQ=60)
                                                                                                                                                 ATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCC    >  SRR19973063.479487/1‑150 (MQ=60)
                                                                                                                                                 ATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCC    <  SRR19973063.2693481/150‑1 (MQ=60)
                                                                                                                                                 ATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCC    <  SRR19973063.2693482/150‑1 (MQ=60)
                                                                                                                                                  TCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG   <  SRR19973063.3382491/150‑1 (MQ=60)
                                                                                                                                                  TCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG   <  SRR19973063.3382498/150‑1 (MQ=60)
                                                                                                                                                   CAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGG  <  SRR19973063.2936641/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TCTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCGG  >  CP009273/1955253‑1955549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑