Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,955,401 C→A 100% S361S (TCC→TCA)  argS → arginyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,955,4010CA97.2% 596.6 / 6.3 190S361S (TCC→TCA) argSarginyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (92/91);  total (97/93)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

ACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAC  >  CP009273/1955256‑1955545
                                                                                                                                                 |                                                                                                                                                
acacCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGACTCAGt                                                                                                                                                >  2:1762627/1‑148 (MQ=255)
 caccaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTa                                                                                                                                               <  2:269026/148‑1 (MQ=255)
 caccaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTa                                                                                                                                               <  1:2239184/148‑1 (MQ=255)
  acccccacTGATATCGCCGGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTAc                                                                                                                                              <  2:2428346/148‑1 (MQ=255)
       cacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTg                                                                                                                                         >  1:1116600/1‑148 (MQ=255)
       cacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGAACGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACATGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTg                                                                                                                                         >  1:3037791/1‑148 (MQ=255)
       cacTGATATCGCATGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTg                                                                                                                                         >  1:2612815/1‑148 (MQ=255)
          tGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGaa                                                                                                                                      <  1:875952/148‑1 (MQ=255)
                  ccTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACAt                                                                                                                              <  2:2966525/148‑1 (MQ=255)
                   cTGTGCGAAATATCTTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATg                                                                                                                             <  2:3496006/148‑1 (MQ=255)
                   cTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATg                                                                                                                             <  2:2210231/148‑1 (MQ=255)
                   cTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATg                                                                                                                             <  1:2238166/148‑1 (MQ=255)
                   cTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATg                                                                                                                             <  1:400315/148‑1 (MQ=255)
                   cTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATg                                                                                                                             <  1:472927/148‑1 (MQ=255)
                   cTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATg                                                                                                                             <  1:472928/148‑1 (MQ=255)
                     gtgCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGtt                                                                                                                           <  1:2566579/148‑1 (MQ=255)
                          aaaTATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCa                                                                                                                      <  2:480153/148‑1 (MQ=255)
                          aaaTATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCa                                                                                                                      <  2:2246047/148‑1 (MQ=255)
                            atatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatg                                                                                                                    >  1:2880712/1‑148 (MQ=255)
                            atatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatg                                                                                                                    >  1:2880748/1‑148 (MQ=255)
                              atCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgat                                                                                                                  >  2:3299751/1‑148 (MQ=255)
                              atCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgat                                                                                                                  <  2:1485399/148‑1 (MQ=255)
                               tCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                 >  2:1062198/1‑148 (MQ=255)
                               tCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                 <  1:278181/148‑1 (MQ=255)
                               tCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                 <  1:2732374/148‑1 (MQ=255)
                               tCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                 <  2:741316/148‑1 (MQ=255)
                               tCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                 <  2:2523794/148‑1 (MQ=255)
                                cGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGc                                                                                                                <  1:1907671/148‑1 (MQ=255)
                                cGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGc                                                                                                                <  2:91235/148‑1 (MQ=255)
                                cGTTAAGAAACACTGCATGCCGATAGCGTGCTGTAATACATCGACTCCAGTCAGCATCAACACCTTATGCAGGCATGTGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGAACCGCTGGAACACCACCTGTGAGGCatgagtc                                                                                                                >  2:2315761/1‑145 (MQ=255)
                                     tGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGt                                                                                                           >  1:902740/1‑148 (MQ=255)
                                       aaaCACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaa                                                                                                         >  2:2476201/1‑148 (MQ=255)
                                        aaCACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaaa                                                                                                        <  1:1328586/148‑1 (MQ=255)
                                         acacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTTTTGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAg                                                                                                       <  1:1092269/148‑1 (MQ=255)
                                         acacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAg                                                                                                       <  2:1588125/148‑1 (MQ=255)
                                         acacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAg                                                                                                       <  2:2031654/148‑1 (MQ=255)
                                         acacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAg                                                                                                       <  1:3231474/148‑1 (MQ=255)
                                          cacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGa                                                                                                      <  1:420013/148‑1 (MQ=255)
                                           acTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGAc                                                                                                     >  2:2007895/1‑148 (MQ=255)
                                                aTGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCaa                                                                                                >  1:3401920/1‑148 (MQ=255)
                                                     gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGt                                                                                           <  2:1286944/148‑1 (MQ=255)
                                                     gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGt                                                                                           >  1:1347874/1‑148 (MQ=255)
                                                     gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGt                                                                                           >  2:1252053/1‑148 (MQ=255)
                                                     gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGt                                                                                           >  1:825611/1‑148 (MQ=255)
                                                      aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGtt                                                                                          <  1:2476201/148‑1 (MQ=255)
                                                      aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGtt                                                                                          >  2:1060498/1‑148 (MQ=255)
                                                      aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGtt                                                                                          >  1:858057/1‑148 (MQ=255)
                                                      aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGtt                                                                                          >  2:3162674/1‑148 (MQ=255)
                                                        cgcgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCa                                                                                        <  2:197409/148‑1 (MQ=255)
                                                          cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCaaa                                                                                      <  1:335801/148‑1 (MQ=255)
                                                             gCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAAcc                                                                                   >  2:1808245/1‑148 (MQ=255)
                                                             gCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAAcc                                                                                   >  2:1808271/1‑148 (MQ=255)
                                                                  aTTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCcgcgc                                                                              >  2:133151/1‑148 (MQ=255)
                                                                  aTTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCcgcgc                                                                              >  1:1021428/1‑148 (MQ=255)
                                                                    tACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCgg                                                                            <  2:2739949/148‑1 (MQ=255)
                                                                    tACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCgg                                                                            <  2:2540329/148‑1 (MQ=255)
                                                                     aCATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCggg                                                                           <  2:2449523/148‑1 (MQ=255)
                                                                     aCATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCggg                                                                           <  2:687624/148‑1 (MQ=255)
                                                                     aCATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCggg                                                                           <  1:1758329/148‑1 (MQ=255)
                                                                        tCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGggtgg                                                                        >  1:3494888/1‑148 (MQ=255)
                                                                         cGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTGCAAAACCCGCGCGggtggt                                                                       >  1:1599890/1‑148 (MQ=255)
                                                                           aCTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTAc                                                                     <  2:2157733/148‑1 (MQ=255)
                                                                             tCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAg                                                                   >  1:1611477/1‑148 (MQ=255)
                                                                              cccGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGt                                                                  >  1:1005076/1‑148 (MQ=255)
                                                                              cccGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGt                                                                  <  2:699792/148‑1 (MQ=255)
                                                                                   cAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAAc                                                             >  1:1579429/1‑148 (MQ=255)
                                                                                    aGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACt                                                            <  1:960186/148‑1 (MQ=255)
                                                                                     gaaTCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTg                                                           >  2:2840539/3‑148 (MQ=255)
                                                                                     gCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTg                                                           >  2:2694919/1‑148 (MQ=255)
                                                                                       aTCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGc                                                         <  1:941512/148‑1 (MQ=255)
                                                                                            cacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATc                                                    >  1:1382421/1‑148 (MQ=255)
                                                                                            cacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATc                                                    <  2:2895449/148‑1 (MQ=255)
                                                                                              ccTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctg                                                  >  1:353441/1‑148 (MQ=255)
                                                                                                tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctgct                                                >  2:3043694/1‑148 (MQ=255)
                                                                                                tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctgct                                                <  2:990942/148‑1 (MQ=255)
                                                                                                tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctgct                                                >  1:956763/1‑148 (MQ=255)
                                                                                                     cAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATg                                           >  1:269084/1‑148 (MQ=255)
                                                                                                                gATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGaa                                <  1:1184384/148‑1 (MQ=255)
                                                                                                                 aTCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAGGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAc                               >  1:1975147/1‑148 (MQ=255)
                                                                                                                 aTCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAc                               >  1:894077/1‑148 (MQ=255)
                                                                                                                  tCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACg                              >  1:682927/1‑148 (MQ=255)
                                                                                                                  tCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACg                              >  1:1485574/1‑148 (MQ=255)
                                                                                                                  tCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACg                              >  1:2542281/1‑148 (MQ=255)
                                                                                                                  tCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACg                              >  1:1486559/1‑148 (MQ=255)
                                                                                                                        gTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACg                        >  2:814686/1‑148 (MQ=255)
                                                                                                                            aGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGt                    <  1:120567/148‑1 (MQ=255)
                                                                                                                            aGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGt                    >  2:3515691/1‑148 (MQ=255)
                                                                                                                             gCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                   >  2:2214025/1‑148 (MQ=255)
                                                                                                                             gCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                   >  2:3503545/1‑148 (MQ=255)
                                                                                                                              cAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCt                  <  2:2510746/148‑1 (MQ=255)
                                                                                                                               aGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtg                 >  1:1677579/1‑148 (MQ=255)
                                                                                                                                ggCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtgg                <  1:1321908/148‑1 (MQ=255)
                                                                                                                                ggCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtgg                >  2:1822046/1‑148 (MQ=255)
                                                                                                                                 gCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtggt               >  1:1950266/1‑148 (MQ=255)
                                                                                                                                      gTACCTGAATCAGTACCGCTGGAACACCACATGTTCTGCATGATTCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAg          <  2:3037791/148‑1 (MQ=255)
                                                                                                                                          cGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCACACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGaaaa      >  2:3440828/1‑148 (MQ=255)
                                                                                                                                           ggAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAg     <  2:386112/148‑1 (MQ=255)
                                                                                                                                            gAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGa    >  1:568076/1‑148 (MQ=255)
                                                                                                                                             aaTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGaa   >  2:411834/1‑148 (MQ=255)
                                                                                                                                             aaTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGaa   <  1:2528286/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAC  >  CP009273/1955256‑1955545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG  >  CP009273/1955254‑1955548
                                                                                                                                                   |                                                                                                                                                   
CTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGT                                                                                                                                                   <  SRR19973065.2267143/150‑1 (MQ=60)
 TACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTA                                                                                                                                                  <  SRR19973065.1868530/150‑1 (MQ=60)
 TACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTA                                                                                                                                                  <  SRR19973065.2053572/150‑1 (MQ=60)
   CACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACC                                                                                                                                                <  SRR19973065.2253420/150‑1 (MQ=60)
       ACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTG                                                                                                                                            >  SRR19973065.1123890/1‑150 (MQ=60)
       ACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGAACGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACATGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTG                                                                                                                                            >  SRR19973065.3056392/1‑150 (MQ=60)
       ACCACTGATATCGCATGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTG                                                                                                                                            >  SRR19973065.2629010/1‑150 (MQ=60)
           CTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAAC                                                                                                                                        <  SRR19973065.2194753/150‑1 (MQ=60)
            TGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACA                                                                                                                                       <  SRR19973065.881546/150‑1 (MQ=60)
              ATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACC                                                                                                                                     >  SRR19973065.1698926/1‑150 (MQ=60)
               TATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCA                                                                                                                                    >  SRR19973065.2559580/1‑150 (MQ=60)
                  CGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACAT                                                                                                                                 >  SRR19973065.62476/1‑150 (MQ=60)
                     CTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTT                                                                                                                              <  SRR19973065.476162/150‑1 (MQ=60)
                     CTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTT                                                                                                                              <  SRR19973065.476161/150‑1 (MQ=60)
                     CTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTT                                                                                                                              <  SRR19973065.403118/150‑1 (MQ=60)
                     CTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTT                                                                                                                              <  SRR19973065.2252395/150‑1 (MQ=60)
                       GTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCG                                                                                                                            >  SRR19973065.2661152/1‑150 (MQ=60)
                       GTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCG                                                                                                                            <  SRR19973065.2582608/150‑1 (MQ=60)
                            AAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATG                                                                                                                       >  SRR19973065.2898237/1‑150 (MQ=60)
                            AAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATG                                                                                                                       >  SRR19973065.2898382/1‑150 (MQ=60)
                            AAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATG                                                                                                                       >  SRR19973065.2898346/1‑150 (MQ=60)
                             AATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGA                                                                                                                      <  SRR19973065.3402831/150‑1 (MQ=60)
                             AATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGA                                                                                                                      >  SRR19973065.471329/1‑150 (MQ=60)
                                 TCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCT                                                                                                                  <  SRR19973065.2749374/150‑1 (MQ=60)
                                 TCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCT                                                                                                                  <  SRR19973065.280036/150‑1 (MQ=60)
                                  CGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTG                                                                                                                 <  SRR19973065.1920203/150‑1 (MQ=60)
                                     TATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGT                                                                                                              >  SRR19973065.908544/1‑150 (MQ=60)
                                          AACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGA                                                                                                         <  SRR19973065.1337372/150‑1 (MQ=60)
                                           ACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTTTTGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGAC                                                                                                        <  SRR19973065.1099462/150‑1 (MQ=60)
                                           ACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGAC                                                                                                        <  SRR19973065.2971746/150‑1 (MQ=60)
                                           ACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGAC                                                                                                        <  SRR19973065.3250894/150‑1 (MQ=60)
                                           ACACTGCATGCCGATCGCGTGCTGTATTAAATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGAC                                                                                                        <  SRR19973065.1804864/150‑1 (MQ=60)
                                            CACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACG                                                                                                       <  SRR19973065.422943/150‑1 (MQ=60)
                                                GCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAA                                                                                                   >  SRR19973065.3422713/1‑150 (MQ=60)
                                                     CCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGT                                                                                              >  SRR19973065.1356790/1‑150 (MQ=60)
                                                     CCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGT                                                                                              >  SRR19973065.830015/1‑150 (MQ=60)
                                                     CCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGT                                                                                              >  SRR19973065.831023/1‑150 (MQ=60)
                                                      CGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTT                                                                                             >  SRR19973065.863593/1‑150 (MQ=60)
                                                      CGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTT                                                                                             >  SRR19973065.863911/1‑150 (MQ=60)
                                                        ATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCA                                                                                           <  SRR19973065.2491791/150‑1 (MQ=60)
                                                        ATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCA                                                                                           <  SRR19973065.2488305/150‑1 (MQ=60)
                                                          CGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAA                                                                                         <  SRR19973065.260913/150‑1 (MQ=60)
                                                            CGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAAC                                                                                       <  SRR19973065.338150/150‑1 (MQ=60)
                                                              TGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCC                                                                                     >  SRR19973065.3071640/1‑150 (MQ=60)
                                                                CTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGC                                                                                   >  SRR19973065.1563720/1‑150 (MQ=60)
                                                                  GTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGC                                                                                 >  SRR19973065.1028200/1‑150 (MQ=60)
                                                                  GTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGC                                                                                 >  SRR19973065.1028247/1‑150 (MQ=60)
                                                                       ACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTG                                                                            <  SRR19973065.1769882/150‑1 (MQ=60)
                                                                        CATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGG                                                                           >  SRR19973065.1981366/1‑150 (MQ=60)
                                                                        CATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGG                                                                           >  SRR19973065.3516131/1‑150 (MQ=60)
                                                                         ATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTGCAAAACCCGCGCGGGTGGT                                                                          >  SRR19973065.1610582/1‑150 (MQ=60)
                                                                         ATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGT                                                                          >  SRR19973065.1297722/1‑150 (MQ=60)
                                                                            GACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACA                                                                       >  SRR19973065.409748/1‑150 (MQ=60)
                                                                             ACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAG                                                                      >  SRR19973065.1622246/1‑150 (MQ=60)
                                                                             ACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAG                                                                      >  SRR19973065.1622291/1‑150 (MQ=60)
                                                                              CTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGT                                                                     >  SRR19973065.1011750/1‑150 (MQ=60)
                                                                              CTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGT                                                                     >  SRR19973065.2774365/1‑150 (MQ=60)
                                                                                   GTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAAC                                                                >  SRR19973065.1589953/1‑150 (MQ=60)
                                                                                      AGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGG                                                             <  SRR19973065.966552/150‑1 (MQ=60)
                                                                                       GCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGC                                                            <  SRR19973065.1109506/150‑1 (MQ=60)
                                                                                        CATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCC                                                           <  SRR19973065.1609460/150‑1 (MQ=60)
                                                                                         ATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCG                                                          <  SRR19973065.947733/150‑1 (MQ=60)
                                                                                         ATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCG                                                          >  SRR19973065.2526523/1‑150 (MQ=60)
                                                                                            AACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATC                                                       <  SRR19973065.3452128/150‑1 (MQ=60)
                                                                                            AACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATC                                                       >  SRR19973065.1391521/1‑150 (MQ=60)
                                                                                             ACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCT                                                      >  SRR19973065.890531/1‑150 (MQ=60)
                                                                                              CACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTG                                                     <  SRR19973065.961426/150‑1 (MQ=60)
                                                                                              CACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTG                                                     >  SRR19973065.355919/1‑150 (MQ=60)
                                                                                              CACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTG                                                     >  SRR19973065.355894/1‑150 (MQ=60)
                                                                                              CACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTG                                                     >  SRR19973065.1442036/1‑150 (MQ=60)
                                                                                               ACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGC                                                    >  SRR19973065.2732626/1‑150 (MQ=60)
                                                                                                CCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCT                                                   >  SRR19973065.963097/1‑150 (MQ=60)
                                                                                                    ATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGAT                                               <  SRR19973065.134295/150‑1 (MQ=60)
                                                                                                     TGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATG                                              >  SRR19973065.270908/1‑150 (MQ=60)
                                                                                                       CAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAA                                            >  SRR19973065.85848/1‑150 (MQ=60)
                                                                                                         GGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGC                                          <  SRR19973065.378850/150‑1 (MQ=60)
                                                                                                                 CGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAGGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAC                                  >  SRR19973065.1988005/1‑150 (MQ=60)
                                                                                                                 CGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAC                                  >  SRR19973065.899938/1‑150 (MQ=60)
                                                                                                                 CGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAAC                                  >  SRR19973065.899756/1‑150 (MQ=60)
                                                                                                                  GATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACG                                 >  SRR19973065.687468/1‑150 (MQ=60)
                                                                                                                  GATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACG                                 <  SRR19973065.1191743/150‑1 (MQ=60)
                                                                                                                  GATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACG                                 <  SRR19973065.1191883/150‑1 (MQ=60)
                                                                                                                  GATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACG                                 >  SRR19973065.2558180/1‑150 (MQ=60)
                                                                                                                  GATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACG                                 >  SRR19973065.1496055/1‑150 (MQ=60)
                                                                                                                  GATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACG                                 >  SRR19973065.1495053/1‑150 (MQ=60)
                                                                                                                  GATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACG                                 >  SRR19973065.2558203/1‑150 (MQ=60)
                                                                                                                     CGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGC                              >  SRR19973065.936179/1‑150 (MQ=60)
                                                                                                                          GTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCC                         >  SRR19973065.1358632/1‑150 (MQ=60)
                                                                                                                              AGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCT                     <  SRR19973065.121625/150‑1 (MQ=60)
                                                                                                                              AGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCT                     <  SRR19973065.121623/150‑1 (MQ=60)
                                                                                                                               GCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTG                    >  SRR19973065.1687816/1‑150 (MQ=60)
                                                                                                                               GCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTG                    >  SRR19973065.1688797/1‑150 (MQ=60)
                                                                                                                                 AGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGT                  >  SRR19973065.1962783/1‑150 (MQ=60)
                                                                                                                                 AGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGT                  >  SRR19973065.1963013/1‑150 (MQ=60)
                                                                                                                                  GGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTG                 <  SRR19973065.1330651/150‑1 (MQ=60)
                                                                                                                                  GGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTG                 <  SRR19973065.1330656/150‑1 (MQ=60)
                                                                                                                                            CGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGA       >  SRR19973065.571675/1‑150 (MQ=60)
                                                                                                                                            CGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGA       >  SRR19973065.571652/1‑150 (MQ=60)
                                                                                                                                             GGACTCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGGTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAccgaa      >  SRR19973065.1312757/1‑145 (MQ=60)
                                                                                                                                              GAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAC     <  SRR19973065.3319851/150‑1 (MQ=60)
                                                                                                                                               AATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACC    >  SRR19973065.1072414/1‑150 (MQ=60)
                                                                                                                                               AATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACC    <  SRR19973065.2544155/150‑1 (MQ=60)
                                                                                                                                                ATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCC   >  SRR19973065.3188156/1‑150 (MQ=60)
                                                                                                                                                ATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCC   >  SRR19973065.3188154/1‑150 (MQ=60)
                                                                                                                                                ATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCC   >  SRR19973065.1432467/1‑150 (MQ=60)
                                                                                                                                                 TCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG  >  SRR19973065.950746/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
CTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCCG  >  CP009273/1955254‑1955548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑