Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,733,753 C→T 100% E253K (GAG→AAG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,733,7530CT99.2% 881.1 / ‑2.8 262E253K (GAG→AAG) aroF3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (155/105);  total (157/105)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 265 total aligned reads displayed.

TCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  CP009273/2733606‑2733898
                                                                                                                                                   |                                                                                                                                                 
tcatcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCtttt                                                                                                                                                 >  2:3374215/1‑150 (MQ=255)
 catcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttt                                                                                                                                                >  2:2398270/1‑150 (MQ=255)
 catcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttt                                                                                                                                                <  1:2669582/150‑1 (MQ=255)
     aGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTca                                                                                                                                            >  1:3450006/1‑150 (MQ=255)
     aGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTca                                                                                                                                            >  1:2893174/1‑150 (MQ=255)
        ccAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAt                                                                                                                                         >  2:3606127/1‑150 (MQ=255)
           ataatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgc                                                                                                                                      >  2:2568220/1‑150 (MQ=255)
            taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcg                                                                                                                                     <  2:4753379/150‑1 (MQ=255)
             aatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcgc                                                                                                                                    >  1:16853/1‑150 (MQ=255)
                  aGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACAt                                                                                                                               <  1:1663590/150‑1 (MQ=255)
                        tGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAg                                                                                                                         >  2:5048910/1‑150 (MQ=255)
                           cATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGc                                                                                                                      >  1:1040472/1‑150 (MQ=255)
                           cATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGc                                                                                                                      >  1:4274838/1‑150 (MQ=255)
                             tCTTTGATGTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCta                                                                                                                    <  1:3592673/150‑1 (MQ=255)
                                 tgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGt                                                                                                                >  2:3101729/1‑150 (MQ=255)
                                 tgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGt                                                                                                                <  2:2480032/150‑1 (MQ=255)
                                  gatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGtt                                                                                                               >  1:4701597/1‑150 (MQ=255)
                                  gatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGtt                                                                                                               >  1:1370512/1‑150 (MQ=255)
                                   atttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTc                                                                                                              >  2:373703/1‑150 (MQ=255)
                                      tgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgc                                                                                                           >  1:4980114/1‑150 (MQ=255)
                                      tgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgc                                                                                                           <  2:1461181/150‑1 (MQ=255)
                                          cAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTCTAGTTCGGCGCtt                                                                                                       >  2:3388884/1‑150 (MQ=255)
                                            aCCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTa                                                                                                     >  2:66398/1‑150 (MQ=255)
                                            aCCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGATCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTa                                                                                                     >  2:66386/1‑150 (MQ=255)
                                             ccACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTac                                                                                                    <  2:462795/150‑1 (MQ=255)
                                              cACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTacc                                                                                                   >  2:4028815/1‑150 (MQ=255)
                                               aCGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTacca                                                                                                  <  1:1637969/150‑1 (MQ=255)
                                               aCGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTacca                                                                                                  >  1:3244373/1‑150 (MQ=255)
                                                cGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTaccac                                                                                                 >  2:248603/1‑150 (MQ=255)
                                                    ttCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTCTAGTTCGGCGCTTTACCACcgcg                                                                                             >  2:2876546/1‑150 (MQ=255)
                                                    ttCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACcgcg                                                                                             >  2:2033108/1‑150 (MQ=255)
                                                      cTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCa                                                                                           <  2:3347296/150‑1 (MQ=255)
                                                          cACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGAt                                                                                       >  2:763543/1‑150 (MQ=255)
                                                          cACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGAt                                                                                       <  2:1659046/150‑1 (MQ=255)
                                                            ccGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATca                                                                                     <  1:3026902/150‑1 (MQ=255)
                                                             cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcac                                                                                    <  1:3259559/150‑1 (MQ=255)
                                                             cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcac                                                                                    <  2:2333969/150‑1 (MQ=255)
                                                             cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcac                                                                                    >  2:1733483/1‑150 (MQ=255)
                                                                aGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATg                                                                                 <  2:3992122/150‑1 (MQ=255)
                                                                 ggCTGACGGCGATTATCTTTATTGGAATGACCGTGGCTGCAATCTAGCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATgg                                                                                <  2:940568/150‑1 (MQ=255)
                                                                 ggCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATgg                                                                                >  1:457751/1‑150 (MQ=255)
                                                                          cGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGa                                                                       >  2:377635/1‑150 (MQ=255)
                                                                          cGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGa                                                                       >  2:2970197/1‑150 (MQ=255)
                                                                           gATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAt                                                                      <  2:1087214/150‑1 (MQ=255)
                                                                            aTAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAtt                                                                     >  1:2536394/1‑150 (MQ=255)
                                                                            aTAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAtt                                                                     >  2:2564227/1‑150 (MQ=255)
                                                                              aaTCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACCTTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACAAGGCCGTCCGGATTcc                                                                   >  1:2722287/1‑150 (MQ=255)
                                                                              aaTCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTcc                                                                   >  2:154577/1‑150 (MQ=255)
                                                                                        ggAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTg                                                         >  1:5094957/1‑150 (MQ=255)
                                                                                         gAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGt                                                        >  2:416098/1‑150 (MQ=255)
                                                                                          aaTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTa                                                       >  1:986323/1‑150 (MQ=255)
                                                                                           aTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAg                                                      >  1:614957/1‑150 (MQ=255)
                                                                                           aTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAg                                                      >  1:3201437/1‑150 (MQ=255)
                                                                                             tACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgca                                                    >  2:2739550/1‑150 (MQ=255)
                                                                                              aCCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaa                                                   >  1:1133881/1‑150 (MQ=255)
                                                                                                  tGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaacgca                                               <  2:324244/150‑1 (MQ=255)
                                                                                                    tcTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaacgcaac                                             <  2:878218/149‑1 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                           >  2:185436/1‑150 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                           <  2:1794930/150‑1 (MQ=255)
                                                                                                       gCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctg                                          >  1:4917530/1‑150 (MQ=255)
                                                                                                         aaTCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgcc                                        >  2:3623316/1‑150 (MQ=255)
                                                                                                           tCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccct                                      >  2:2106589/1‑150 (MQ=255)
                                                                                                             tACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctgc                                    >  1:1160055/1‑150 (MQ=255)
                                                                                                              aCCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACctgccctgcc                                   <  1:2048917/150‑1 (MQ=255)
                                                                                                               ccATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCcctgcct                                  >  2:1402218/1‑150 (MQ=255)
                                                                                                               acATCAGAGACGGGCGCAGTCCCGACTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGATATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCcctgcct                                  <  1:5072731/149‑1 (MQ=255)
                                                                                                                cATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCcctgcctg                                 >  2:4562473/1‑150 (MQ=255)
                                                                                                                 aTCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTgg                                >  2:4051421/1‑150 (MQ=255)
                                                                                                                 aTCAGAGACGGGCGCAGTCCCGCCTGGTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTATGTAGCAACGCAACCTGCCCTGCCTgg                                >  1:2602933/1‑150 (MQ=255)
                                                                                                                    agagACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                             <  1:2065225/150‑1 (MQ=255)
                                                                                                                    agagACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                             >  2:3199213/1‑150 (MQ=255)
                                                                                                                      agaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAAt                           >  2:3922705/1‑150 (MQ=255)
                                                                                                                       gaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                          >  2:3000205/1‑150 (MQ=255)
                                                                                                                       gaCGGGCGCAGTCCCGCCTGGTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                          <  1:2947381/150‑1 (MQ=255)
                                                                                                                         cGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGcc                        <  1:4047698/150‑1 (MQ=255)
                                                                                                                         cGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGcc                        >  1:3357943/1‑150 (MQ=255)
                                                                                                                           ggCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaa                      <  1:1894148/150‑1 (MQ=255)
                                                                                                                            gcgcAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaac                     <  1:4464985/150‑1 (MQ=255)
                                                                                                                             cgcAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaaca                    >  1:240560/1‑150 (MQ=255)
                                                                                                                              gcAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaacaa                   >  2:4213532/1‑150 (MQ=255)
                                                                                                                                aGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa                 <  2:238117/150‑1 (MQ=255)
                                                                                                                                aGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATACCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa                 >  2:2549346/1‑150 (MQ=255)
                                                                                                                                  tCCCGCCTGTTCCATCTTATTTTCACATTGCGTAAGATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACg               <  1:2246873/150‑1 (MQ=255)
                                                                                                                                   cccGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACgg              >  1:171338/1‑150 (MQ=255)
                                                                                                                                    ccGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGt             >  2:96630/1‑150 (MQ=255)
                                                                                                                                    ccGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGt             <  2:1001751/150‑1 (MQ=255)
                                                                                                                                     cGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTg            >  1:2359079/1‑150 (MQ=255)
                                                                                                                                      gCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGc           <  1:4865488/150‑1 (MQ=255)
                                                                                                                                       ccTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCg          >  2:4390271/1‑150 (MQ=255)
                                                                                                                                       ccTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCg          <  2:1094004/150‑1 (MQ=255)
                                                                                                                                       ccTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCg          <  2:2286025/150‑1 (MQ=255)
                                                                                                                                        cTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCgg         >  1:3854108/1‑150 (MQ=255)
                                                                                                                                          gTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCt       <  2:4794642/150‑1 (MQ=255)
                                                                                                                                          gTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCt       <  2:600255/150‑1 (MQ=255)
                                                                                                                                           ttCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTg      <  1:3028177/150‑1 (MQ=255)
                                                                                                                                           ttCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTg      >  1:127663/1‑150 (MQ=255)
                                                                                                                                            tCCATCTTTTTTTCACCTTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGGTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTgg     <  2:333895/150‑1 (MQ=255)
                                                                                                                                            tCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTgg     <  1:1008129/150‑1 (MQ=255)
                                                                                                                                              cATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggc   <  2:2757995/150‑1 (MQ=255)
                                                                                                                                              cATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggc   <  1:4027744/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  CP009273/2733606‑2733898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGG  >  CP009273/2733604‑2733902
                                                                                                                                                      |                                                                                                                                                     
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTT                                                                                                                                                       >  SRR19973063.70214/1‑150 (MQ=60)
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTT                                                                                                                                                       <  SRR19973063.1340458/150‑1 (MQ=60)
   CATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTT                                                                                                                                                    <  SRR19973063.2678157/150‑1 (MQ=60)
       AGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCA                                                                                                                                                >  SRR19973063.2902406/1‑150 (MQ=60)
       AGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCA                                                                                                                                                >  SRR19973063.3460973/1‑150 (MQ=60)
        GACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCAC                                                                                                                                               >  SRR19973063.2010176/1‑150 (MQ=60)
        GACCAATAATTGAGCGATTGCCATCTTTGATTTGAGAAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCAC                                                                                                                                               <  SRR19973063.2451091/150‑1 (MQ=60)
          CCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAT                                                                                                                                             <  SRR19973063.1013472/150‑1 (MQ=60)
            AATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTG                                                                                                                                           <  SRR19973063.4206215/150‑1 (MQ=60)
            AATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTG                                                                                                                                           <  SRR19973063.2501019/150‑1 (MQ=60)
             ATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGC                                                                                                                                          >  SRR19973063.4314930/1‑150 (MQ=60)
               AATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGC                                                                                                                                        >  SRR19973063.16918/1‑150 (MQ=60)
                  TGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAAC                                                                                                                                     >  SRR19973063.1097334/1‑150 (MQ=60)
                  TGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAAC                                                                                                                                     >  SRR19973063.1678379/1‑150 (MQ=60)
                  TGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAAC                                                                                                                                     >  SRR19973063.1678432/1‑150 (MQ=60)
                    AGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACAT                                                                                                                                   <  SRR19973063.1668663/150‑1 (MQ=60)
                      CGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGACTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCC                                                                                                                                 >  SRR19973063.334858/1‑150 (MQ=60)
                      CGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCC                                                                                                                                 <  SRR19973063.3707463/150‑1 (MQ=60)
                          TGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAG                                                                                                                             >  SRR19973063.3889469/1‑150 (MQ=60)
                           GCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGAGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTTCGCAACATCCGCAGG                                                                                                                            >  SRR19973063.2170855/1‑150 (MQ=60)
                            CCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGG                                                                                                                           <  SRR19973063.4934670/150‑1 (MQ=60)
                             CATCTTTGATTTGAGCAACCACGGATTTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGG                                                                                                                           >  SRR19973063.1043812/1‑150 (MQ=60)
                             CATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGC                                                                                                                          >  SRR19973063.4288429/1‑150 (MQ=60)
                             CATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGC                                                                                                                          >  SRR19973063.1043624/1‑150 (MQ=60)
                               TCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTA                                                                                                                        <  SRR19973063.3155297/150‑1 (MQ=60)
                               TCTTTGATGTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTA                                                                                                                        <  SRR19973063.3604193/150‑1 (MQ=60)
                                CTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTAT                                                                                                                       >  SRR19973063.3518120/1‑150 (MQ=60)
                                    GATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTT                                                                                                                   >  SRR19973063.4716580/1‑150 (MQ=60)
                                    GATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTT                                                                                                                   >  SRR19973063.2139479/1‑150 (MQ=60)
                                    GATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTT                                                                                                                   >  SRR19973063.1374645/1‑150 (MQ=60)
                                     ATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTC                                                                                                                  <  SRR19973063.1192004/150‑1 (MQ=60)
                                        TGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGC                                                                                                               >  SRR19973063.4996212/1‑150 (MQ=60)
                                         GAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCG                                                                                                              <  SRR19973063.459872/150‑1 (MQ=60)
                                             AACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTT                                                                                                          >  SRR19973063.4781564/1‑150 (MQ=60)
                                                 ACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCA                                                                                                      <  SRR19973063.1642979/150‑1 (MQ=60)
                                                 ACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCA                                                                                                      >  SRR19973063.3254791/1‑150 (MQ=60)
                                                 ACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCACCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCA                                                                                                      >  SRR19973063.3254793/1‑150 (MQ=60)
                                                  CGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCAC                                                                                                     <  SRR19973063.272955/150‑1 (MQ=60)
                                                       TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCG                                                                                                 <  SRR19973063.5013303/150‑1 (MQ=60)
                                                         CTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCA                                                                                               >  SRR19973063.4561433/1‑150 (MQ=60)
                                                          TGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAG                                                                                              <  SRR19973063.3636034/150‑1 (MQ=60)
                                                               CCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCA                                                                                         <  SRR19973063.3036551/150‑1 (MQ=60)
                                                                CGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCAC                                                                                        <  SRR19973063.3270022/150‑1 (MQ=60)
                                                                CGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCAC                                                                                        >  SRR19973063.2989865/1‑150 (MQ=60)
                                                                 GCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACA                                                                                       <  SRR19973063.1809355/150‑1 (MQ=60)
                                                                   AGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATG                                                                                     >  SRR19973063.2391525/1‑150 (MQ=60)
                                                                    GGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGG                                                                                    >  SRR19973063.459040/1‑150 (MQ=60)
                                                                         ACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTC                                                                               <  SRR19973063.1292172/150‑1 (MQ=60)
                                                                          CGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCC                                                                              <  SRR19973063.3358673/150‑1 (MQ=60)
                                                                              GATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGAT                                                                          <  SRR19973063.3013594/150‑1 (MQ=60)
                                                                               ATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATT                                                                         >  SRR19973063.2544480/1‑150 (MQ=60)
                                                                                 AATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACCTTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACAAGGCCGTCCGGATTCC                                                                       >  SRR19973063.2731077/1‑150 (MQ=60)
                                                                                       TATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAG                                                                 >  SRR19973063.3042563/1‑150 (MQ=60)
                                                                                          TGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTT                                                              >  SRR19973063.150637/1‑150 (MQ=60)
                                                                                           GGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTG                                                             >  SRR19973063.5111350/1‑150 (MQ=60)
                                                                                             AATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTA                                                           >  SRR19973063.989297/1‑150 (MQ=60)
                                                                                              ATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAG                                                          >  SRR19973063.3211731/1‑150 (MQ=60)
                                                                                              ATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAG                                                          >  SRR19973063.616763/1‑150 (MQ=60)
                                                                                               TTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTGGGTAGC                                                         >  SRR19973063.2953691/1‑150 (MQ=60)
                                                                                                TACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCA                                                        >  SRR19973063.996533/1‑150 (MQ=60)
                                                                                                 ACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAA                                                       >  SRR19973063.1137318/1‑150 (MQ=60)
                                                                                                    GTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGC                                                    >  SRR19973063.2790876/1‑150 (MQ=60)
                                                                                                        CTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACC                                                >  SRR19973063.4615950/1‑150 (MQ=60)
                                                                                                        CTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACC                                                <  SRR19973063.4302172/150‑1 (MQ=60)
                                                                                                        CTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACC                                                <  SRR19973063.2332628/150‑1 (MQ=60)
                                                                                                         TGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCT                                               <  SRR19973063.26357/150‑1 (MQ=60)
                                                                                                          GCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTG                                              >  SRR19973063.4933408/1‑150 (MQ=60)
                                                                                                            AATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCC                                            >  SRR19973063.2719843/1‑150 (MQ=60)
                                                                                                             ATCTACCATCAGAGACGGGCGCAGTCCCGCCTG‑TCCATCTTTTTTTCACAGTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCT                                          <  SRR19973063.4946797/150‑1 (MQ=60)
                                                                                                                TACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGC                                        >  SRR19973063.1664277/1‑150 (MQ=60)
                                                                                                                TACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGC                                        >  SRR19973063.1163554/1‑150 (MQ=60)
                                                                                                                 ACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCC                                       <  SRR19973063.2055317/150‑1 (MQ=60)
                                                                                                                 ACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCC                                       <  SRR19973063.2055328/150‑1 (MQ=60)
                                                                                                                  CCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCT                                      <  SRR19973063.5089110/150‑1 (MQ=60)
                                                                                                                  ACATCAGAGACGGGCGCAGTCCCGACTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGATATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCT                                      <  SRR19973063.5089048/150‑1 (MQ=60)
                                                                                                                    ATCAGAGACGGGCGCAGTCCCGCCTGGTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTATGTAGCAACGCAACCTGCCCTGCCTGG                                    >  SRR19973063.2611207/1‑150 (MQ=60)
                                                                                                                      CAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTT                                  <  SRR19973063.671719/150‑1 (MQ=60)
                                                                                                                       AGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTA                                 <  SRR19973063.2071677/150‑1 (MQ=60)
                                                                                                                       AGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTA                                 >  SRR19973063.5040370/1‑150 (MQ=60)
                                                                                                                       AGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTA                                 >  SRR19973063.1137782/1‑150 (MQ=60)
                                                                                                                       AGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTA                                 <  SRR19973063.2572396/150‑1 (MQ=60)
                                                                                                                       AGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTA                                 <  SRR19973063.2572746/150‑1 (MQ=60)
                                                                                                                          GACGGGCGCAGTCCCGCCTGGTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATG                              <  SRR19973063.2956803/150‑1 (MQ=60)
                                                                                                                            CGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCC                            <  SRR19973063.4060666/150‑1 (MQ=60)
                                                                                                                            CGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCC                            >  SRR19973063.3368648/1‑150 (MQ=60)
                                                                                                                            CGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCC                            >  SRR19973063.3368632/1‑150 (MQ=60)
                                                                                                                              GGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAA                          <  SRR19973063.1664309/150‑1 (MQ=60)
                                                                                                                              GGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAA                          <  SRR19973063.1900072/150‑1 (MQ=60)
                                                                                                                               GCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAAC                         >  SRR19973063.1705432/1‑150 (MQ=60)
                                                                                                                               GCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAAC                         >  SRR19973063.3887540/1‑150 (MQ=60)
                                                                                                                               GCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAAC                         <  SRR19973063.4479312/150‑1 (MQ=60)
                                                                                                                               GCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACACAACCTGCCCTGCCTGGTTAATGCCAAC                         <  SRR19973063.199238/150‑1 (MQ=60)
                                                                                                                                CGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACA                        >  SRR19973063.241288/1‑150 (MQ=60)
                                                                                                                                  CAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAA                      >  SRR19973063.4917985/1‑150 (MQ=60)
                                                                                                                                     TCCCGCCTGTTCCATCTTATTTTCACATTGCGTAAGATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACG                   <  SRR19973063.2254019/150‑1 (MQ=60)
                                                                                                                                      CCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGG                  >  SRR19973063.4841649/1‑150 (MQ=60)
                                                                                                                                      CCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGG                  >  SRR19973063.171868/1‑150 (MQ=60)
                                                                                                                                       CCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGT                 >  SRR19973063.993776/1‑150 (MQ=60)
                                                                                                                                        CGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTG                >  SRR19973063.2366630/1‑150 (MQ=60)
                                                                                                                                         GCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGC               >  SRR19973063.1841304/1‑150 (MQ=60)
                                                                                                                                         GCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGC               <  SRR19973063.4881168/150‑1 (MQ=60)
                                                                                                                                           CTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGG             >  SRR19973063.3866360/1‑150 (MQ=60)
                                                                                                                                             GTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCT           >  SRR19973063.3369709/1‑150 (MQ=60)
                                                                                                                                             GTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCT           <  SRR19973063.1342637/150‑1 (MQ=60)
                                                                                                                                              TTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTG          >  SRR19973063.128076/1‑150 (MQ=60)
                                                                                                                                              TTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTG          >  SRR19973063.128060/1‑150 (MQ=60)
                                                                                                                                              TTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTG          <  SRR19973063.3037834/150‑1 (MQ=60)
                                                                                                                                              TTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTG          <  SRR19973063.4042270/150‑1 (MQ=60)
                                                                                                                                              TTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTG          <  SRR19973063.3037775/150‑1 (MQ=60)
                                                                                                                                               TCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGG         >  SRR19973063.3217464/1‑150 (MQ=60)
                                                                                                                                               TCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGG         >  SRR19973063.3217338/1‑150 (MQ=60)
                                                                                                                                               TCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGG         <  SRR19973063.1011167/150‑1 (MQ=60)
                                                                                                                                                 CATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGC       <  SRR19973063.4040658/150‑1 (MQ=60)
                                                                                                                                                 CATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGC       >  SRR19973063.228759/1‑150 (MQ=60)
                                                                                                                                                 CATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGC       >  SRR19973063.2821183/1‑150 (MQ=60)
                                                                                                                                                  ATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG      <  SRR19973063.4149419/150‑1 (MQ=60)
                                                                                                                                                  ATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG      <  SRR19973063.611614/150‑1 (MQ=60)
                                                                                                                                                     TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCG   <  SRR19973063.544439/150‑1 (MQ=60)
                                                                                                                                                     TTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCG   <  SRR19973063.3111710/150‑1 (MQ=60)
                                                                                                                                                      TTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACAGGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGG  <  SRR19973063.4389076/150‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                     
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGA‑TTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGG  >  CP009273/2733604‑2733902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑