Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,733,753 C→T 100% E253K (GAG→AAG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,733,7530CT100.0% 762.7 / NA 211E253K (GAG→AAG) aroF3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (122/89);  total (122/89)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 225 total aligned reads displayed.

TCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  CP009273/2733606‑2733898
                                                                                                                                                   |                                                                                                                                                 
tcatcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCtttt                                                                                                                                                 >  2:803966/1‑150 (MQ=255)
tcatcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCtttt                                                                                                                                                 >  2:2303475/1‑150 (MQ=255)
 catcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttt                                                                                                                                                <  1:1000474/150‑1 (MQ=255)
 catcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttt                                                                                                                                                <  1:1550130/150‑1 (MQ=255)
  atcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCtttttt                                                                                                                                               >  1:978560/1‑150 (MQ=255)
  atcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCtttttt                                                                                                                                               >  1:3665886/1‑150 (MQ=255)
   tcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttttt                                                                                                                                              <  1:859219/150‑1 (MQ=255)
   tcaGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTtttttt                                                                                                                                              <  1:2419364/150‑1 (MQ=255)
    caGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTc                                                                                                                                             <  2:3905476/150‑1 (MQ=255)
    caGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTc                                                                                                                                             <  2:3637759/150‑1 (MQ=255)
    caGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTc                                                                                                                                             >  2:1054944/1‑150 (MQ=255)
     aGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTca                                                                                                                                            >  2:923423/1‑150 (MQ=255)
     aGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTca                                                                                                                                            >  2:192195/1‑150 (MQ=255)
         cAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGTGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACAtt                                                                                                                                        <  1:2385684/150‑1 (MQ=255)
            taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcg                                                                                                                                     >  1:208638/1‑150 (MQ=255)
            taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcg                                                                                                                                     >  1:2581180/1‑150 (MQ=255)
            taatTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTgcg                                                                                                                                     >  2:489178/1‑150 (MQ=255)
                  aGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACAt                                                                                                                               >  2:167644/1‑150 (MQ=255)
                  aGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACAt                                                                                                                               >  2:3949674/1‑150 (MQ=255)
                  aGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACAt                                                                                                                               <  2:2676630/150‑1 (MQ=255)
                       ttGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCa                                                                                                                          >  1:525195/1‑150 (MQ=255)
                        tGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAg                                                                                                                         >  1:4012594/1‑150 (MQ=255)
                        tGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAg                                                                                                                         <  1:1993062/150‑1 (MQ=255)
                        tGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAg                                                                                                                         <  2:4014921/150‑1 (MQ=255)
                         gCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAgg                                                                                                                        >  1:2436824/1‑150 (MQ=255)
                           cATCTTTGATTTGAGCAACCACGGATTCTGCCACTGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGc                                                                                                                      >  2:1707449/1‑150 (MQ=255)
                            aTCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCACTCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCt                                                                                                                     >  2:3097474/1‑150 (MQ=255)
                            aTCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCt                                                                                                                     <  1:889766/150‑1 (MQ=255)
                               tttgttttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTTTTGGAATTTCCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCtata                                                                                                                  <  2:1225067/150‑1 (MQ=255)
                               tttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCtata                                                                                                                  >  2:695691/1‑150 (MQ=255)
                                ttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAg                                                                                                                 <  2:2143539/150‑1 (MQ=255)
                                ttgatttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAg                                                                                                                 <  1:2669055/150‑1 (MQ=255)
                                   atttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTc                                                                                                              >  1:1336496/1‑150 (MQ=255)
                                   atttgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTc                                                                                                              <  1:2290088/150‑1 (MQ=255)
                                      tgaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgc                                                                                                           <  1:1118625/150‑1 (MQ=255)
                                       gaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgcg                                                                                                          >  1:1466806/1‑150 (MQ=255)
                                       gaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgcg                                                                                                          >  2:1195711/1‑150 (MQ=255)
                                       gaGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGgcg                                                                                                          >  2:74901/1‑150 (MQ=255)
                                         gCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCt                                                                                                        >  2:3357193/1‑150 (MQ=255)
                                            aCCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTa                                                                                                     >  1:4127897/1‑150 (MQ=255)
                                             ccACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTac                                                                                                    >  2:3563420/1‑150 (MQ=255)
                                                cGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTaccac                                                                                                 >  2:2097780/1‑150 (MQ=255)
                                                 ggATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGTAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTaccacc                                                                                                >  2:2358547/1‑150 (MQ=255)
                                                   aTTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGGTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACcgc                                                                                              >  1:2983945/1‑150 (MQ=255)
                                                       tGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAg                                                                                          >  1:3434243/1‑150 (MQ=255)
                                                             cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcac                                                                                    <  2:3227983/150‑1 (MQ=255)
                                                             cGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATcac                                                                                    <  1:3143334/150‑1 (MQ=255)
                                                                 ggCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATgg                                                                                <  2:2728983/150‑1 (MQ=255)
                                                                   cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGcc                                                                              <  2:3051525/150‑1 (MQ=255)
                                                                   cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGcc                                                                              >  1:2060453/1‑150 (MQ=255)
                                                                   cTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGcc                                                                              <  1:1614349/150‑1 (MQ=255)
                                                                    tGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCg                                                                             <  1:1856569/150‑1 (MQ=255)
                                                                       cGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTcc                                                                          <  1:3270777/150‑1 (MQ=255)
                                                                          cGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGa                                                                       >  2:212972/1‑150 (MQ=255)
                                                                             tAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTc                                                                    <  1:328947/150‑1 (MQ=255)
                                                                             tAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTc                                                                    >  2:1798991/1‑150 (MQ=255)
                                                                              aaTCTATAATGGAATTACCGTTTCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATATTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTcc                                                                   >  2:592674/1‑150 (MQ=255)
                                                                               aTCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTccc                                                                  <  1:624979/150‑1 (MQ=255)
                                                                                  tttATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTg                                                               <  1:3748332/150‑1 (MQ=255)
                                                                                   ttATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTCCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGa                                                              <  2:508549/150‑1 (MQ=255)
                                                                                    tATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAg                                                             <  2:2567287/150‑1 (MQ=255)
                                                                                     aTTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGt                                                            >  2:2232879/1‑150 (MQ=255)
                                                                                      ttGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGtt                                                           <  2:3912686/150‑1 (MQ=255)
                                                                                       tGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATAACATGGCCGTCCGGATTCCCCTGAGttt                                                          >  2:1513219/1‑150 (MQ=255)
                                                                                          aaTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTa                                                       >  2:3638396/1‑150 (MQ=255)
                                                                                          aaTTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTa                                                       >  1:1756194/1‑150 (MQ=255)
                                                                                              aCCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaa                                                   >  2:1227050/1‑150 (MQ=255)
                                                                                               ccGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAgcaac                                                  <  1:290590/150‑1 (MQ=255)
                                                                                                     cTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcc                                            <  2:3461480/150‑1 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                           >  2:2812945/1‑150 (MQ=255)
                                                                                                      tGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcct                                           >  2:3696732/1‑150 (MQ=255)
                                                                                                       gCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctg                                          <  2:160695/150‑1 (MQ=255)
                                                                                                        cAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgc                                         >  2:3434083/1‑150 (MQ=255)
                                                                                                         aaTCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTATTCACATTGCGCAACATCCGCAGGGCTATAGTTCTGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTAGTAGCAACGCAAactgcc                                        >  1:3090520/1‑150 (MQ=255)
                                                                                                            cTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAAcctgccctg                                     <  1:3713441/150‑1 (MQ=255)
                                                                                                               ccATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCcctgcct                                  <  2:2446387/150‑1 (MQ=255)
                                                                                                               ccATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCcctgcct                                  >  1:2550642/1‑150 (MQ=255)
                                                                                                                    agagACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                             >  1:1190970/1‑150 (MQ=255)
                                                                                                                    agagACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTa                             >  1:2850315/1‑150 (MQ=255)
                                                                                                                       gaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                          >  1:2013825/1‑150 (MQ=255)
                                                                                                                       gaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                          <  2:1990909/150‑1 (MQ=255)
                                                                                                                       gaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                          >  2:3037017/1‑150 (MQ=255)
                                                                                                                       gaCGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATg                          >  1:1405858/1‑150 (MQ=255)
                                                                                                                         cGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGcc                        >  1:4160495/1‑150 (MQ=255)
                                                                                                                          gggCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCca                       >  1:4123877/1‑150 (MQ=255)
                                                                                                                           ggCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCcaa                      >  2:46681/1‑150 (MQ=255)
                                                                                                                               cAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaa                  >  1:3870102/1‑150 (MQ=255)
                                                                                                                                aGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa                 >  1:3540088/1‑150 (MQ=255)
                                                                                                                                aGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa                 >  1:2804838/1‑150 (MQ=255)
                                                                                                                                aGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACaaaa                 >  1:1102620/1‑150 (MQ=255)
                                                                                                                                  tCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACg               <  2:1962419/150‑1 (MQ=255)
                                                                                                                                       ccTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCg          <  2:1900865/150‑1 (MQ=255)
                                                                                                                                        cTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCgg         <  2:3165151/150‑1 (MQ=255)
                                                                                                                                          gTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCt       >  1:3935547/1‑150 (MQ=255)
                                                                                                                                          gTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCt       >  1:3469176/1‑150 (MQ=255)
                                                                                                                                           ttCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTg      <  1:2732549/150‑1 (MQ=255)
                                                                                                                                            tCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTgg     >  1:642958/1‑150 (MQ=255)
                                                                                                                                             ccATCTTTTTTTCACATTGCGCACCATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTggg    <  1:430242/150‑1 (MQ=255)
                                                                                                                                              cATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGggc   >  1:1063753/1‑150 (MQ=255)
                                                                                                                                               aTCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAAAGGTGCGGCTGggcg  >  2:2247404/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  CP009273/2733606‑2733898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  CP009273/2733604‑2733898
                                                                                                                                                     |                                                                                                                                                 
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTT                                                                                                                                                   <  SRR19973074.3583452/150‑1 (MQ=60)
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTT                                                                                                                                                   >  SRR19973074.2822404/1‑150 (MQ=60)
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTT                                                                                                                                                   >  SRR19973074.1966169/1‑150 (MQ=60)
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTT                                                                                                                                                   >  SRR19973074.1966165/1‑150 (MQ=60)
 ATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTT                                                                                                                                                  >  SRR19973074.2159035/1‑150 (MQ=60)
   CATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTT                                                                                                                                                <  SRR19973074.1002333/150‑1 (MQ=60)
   CATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTT                                                                                                                                                <  SRR19973074.1553077/150‑1 (MQ=60)
   CATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTT                                                                                                                                                <  SRR19973074.1553060/150‑1 (MQ=60)
    ATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTT                                                                                                                                               >  SRR19973074.980395/1‑150 (MQ=60)
    ATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTT                                                                                                                                               >  SRR19973074.980356/1‑150 (MQ=60)
    ATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTT                                                                                                                                               >  SRR19973074.3672668/1‑150 (MQ=60)
    ATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTT                                                                                                                                               <  SRR19973074.3468629/150‑1 (MQ=60)
     TCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTT                                                                                                                                              >  SRR19973074.787880/1‑150 (MQ=60)
     TCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTT                                                                                                                                              <  SRR19973074.2423840/150‑1 (MQ=60)
     TCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTT                                                                                                                                              <  SRR19973074.860875/150‑1 (MQ=60)
     TCAGACCAATAATTGAGCGATTGCCATATTTGATTTGAGAAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTTGAATTACCGTGGCTGCAATCTACAATCAGAGACGGTCGCAGTCCCGCCTGTTCCATCTTTTTTT                                                                                                                                              <  SRR19973074.2436053/150‑1 (MQ=60)
           CAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGTGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATT                                                                                                                                        <  SRR19973074.2390111/150‑1 (MQ=60)
           CAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATT                                                                                                                                        <  SRR19973074.1923889/150‑1 (MQ=60)
              TAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCG                                                                                                                                     >  SRR19973074.209073/1‑150 (MQ=60)
              TAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCG                                                                                                                                     >  SRR19973074.2586000/1‑150 (MQ=60)
              TAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCG                                                                                                                                     >  SRR19973074.2585626/1‑150 (MQ=60)
                        ATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGC                                                                                                                           >  SRR19973074.3281282/1‑150 (MQ=60)
                         TTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCA                                                                                                                          >  SRR19973074.526242/1‑150 (MQ=60)
                          TGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAG                                                                                                                         >  SRR19973074.1763303/1‑150 (MQ=60)
                          TGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAG                                                                                                                         <  SRR19973074.1996846/150‑1 (MQ=60)
                          TGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAG                                                                                                                         >  SRR19973074.4020016/1‑150 (MQ=60)
                           GCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGG                                                                                                                        >  SRR19973074.2441334/1‑150 (MQ=60)
                           GCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGG                                                                                                                        >  SRR19973074.3736829/1‑150 (MQ=60)
                              ATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCT                                                                                                                     <  SRR19973074.891468/150‑1 (MQ=60)
                               TCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTA                                                                                                                    >  SRR19973074.2473120/1‑150 (MQ=60)
                                  TTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAG                                                                                                                 <  SRR19973074.2673721/150‑1 (MQ=60)
                                  TTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAG                                                                                                                 <  SRR19973074.2674028/150‑1 (MQ=60)
                                     ATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTC                                                                                                              >  SRR19973074.1339207/1‑150 (MQ=60)
                                     ATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTC                                                                                                              <  SRR19973074.2294376/150‑1 (MQ=60)
                                     ATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTC                                                                                                              >  SRR19973074.1338994/1‑150 (MQ=60)
                                        TGAGCAACCACTGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGC                                                                                                           <  SRR19973074.1975080/150‑1 (MQ=60)
                                        TGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTTCCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGC                                                                                                           <  SRR19973074.1120739/150‑1 (MQ=60)
                                        TGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGC                                                                                                           >  SRR19973074.2345246/1‑150 (MQ=60)
                                        TGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGC                                                                                                           <  SRR19973074.1120736/150‑1 (MQ=60)
                                         GAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCG                                                                                                          >  SRR19973074.1469560/1‑150 (MQ=60)
                                            CAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTT                                                                                                       >  SRR19973074.3077875/1‑150 (MQ=60)
                                             AACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTT                                                                                                      <  SRR19973074.1083998/150‑1 (MQ=60)
                                              ACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTA                                                                                                     >  SRR19973074.4135507/1‑150 (MQ=60)
                                               CCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTAC                                                                                                    <  SRR19973074.627000/150‑1 (MQ=60)
                                                 ACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCA                                                                                                  >  SRR19973074.3990137/1‑150 (MQ=60)
                                                     ATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGC                                                                                              >  SRR19973074.2989606/1‑150 (MQ=60)
                                                     ATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGGTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGC                                                                                              >  SRR19973074.2989492/1‑150 (MQ=60)
                                                        CTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCA                                                                                           <  SRR19973074.160353/150‑1 (MQ=60)
                                                         TGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAG                                                                                          >  SRR19973074.3440572/1‑150 (MQ=60)
                                                               CGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCAC                                                                                    <  SRR19973074.3149107/150‑1 (MQ=60)
                                                               CGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCAC                                                                                    <  SRR19973074.3149096/150‑1 (MQ=60)
                                                                     CTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCC                                                                              >  SRR19973074.2064327/1‑150 (MQ=60)
                                                                     CTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCC                                                                              <  SRR19973074.1617417/150‑1 (MQ=60)
                                                                     CTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCC                                                                              <  SRR19973074.327328/150‑1 (MQ=60)
                                                                     CTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCC                                                                              >  SRR19973074.2064323/1‑150 (MQ=60)
                                                                      TGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCG                                                                             <  SRR19973074.1860114/150‑1 (MQ=60)
                                                                      TGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCG                                                                             >  SRR19973074.2129210/1‑150 (MQ=60)
                                                                         CGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCC                                                                          <  SRR19973074.3276843/150‑1 (MQ=60)
                                                                               TAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTC                                                                    <  SRR19973074.1439770/150‑1 (MQ=60)
                                                                               TAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTC                                                                    <  SRR19973074.329544/150‑1 (MQ=60)
                                                                                 ATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCC                                                                  <  SRR19973074.626125/150‑1 (MQ=60)
                                                                                 ATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCC                                                                  >  SRR19973074.2409285/1‑150 (MQ=60)
                                                                                 ATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCC                                                                  <  SRR19973074.626154/150‑1 (MQ=60)
                                                                                    TTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTG                                                               <  SRR19973074.3755226/150‑1 (MQ=60)
                                                                                     TTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGA                                                              >  SRR19973074.2929625/1‑150 (MQ=60)
                                                                                       ATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGT                                                            >  SRR19973074.2550656/1‑150 (MQ=60)
                                                                                           GAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGT                                                        <  SRR19973074.412853/150‑1 (MQ=60)
                                                                                            AATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGTGTTTGTA                                                       <  SRR19973074.3905856/150‑1 (MQ=60)
                                                                                            AATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTA                                                       >  SRR19973074.1759452/1‑150 (MQ=60)
                                                                                            AATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTA                                                       >  SRR19973074.1759464/1‑150 (MQ=60)
                                                                                                 CCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAAC                                                  <  SRR19973074.291132/150‑1 (MQ=60)
                                                                                                     GGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAA                                              <  SRR19973074.2275730/150‑1 (MQ=60)
                                                                                                           AATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTATTCACATTGCGCAACATCCGCAGGGCTATAGTTCTGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTAGTAGCAACGCAAACTGCC                                        >  SRR19973074.3096197/1‑150 (MQ=60)
                                                                                                             TCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCT                                      <  SRR19973074.1477907/150‑1 (MQ=60)
                                                                                                              CTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTG                                     <  SRR19973074.3720301/150‑1 (MQ=60)
                                                                                                              CTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTG                                     <  SRR19973074.3720293/150‑1 (MQ=60)
                                                                                                              CTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTG                                     <  SRR19973074.3606169/150‑1 (MQ=60)
                                                                                                               TACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGC                                    >  SRR19973074.4174626/1‑150 (MQ=60)
                                                                                                               TACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGC                                    >  SRR19973074.4175030/1‑150 (MQ=60)
                                                                                                                ACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCC                                   <  SRR19973074.885413/150‑1 (MQ=60)
                                                                                                                 CCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCT                                  <  SRR19973074.1044525/150‑1 (MQ=60)
                                                                                                                 CCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCT                                  >  SRR19973074.2555403/1‑150 (MQ=60)
                                                                                                                   ATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGG                                >  SRR19973074.3352277/1‑150 (MQ=60)
                                                                                                                      AGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTA                             >  SRR19973074.1193243/1‑150 (MQ=60)
                                                                                                                      AGAGACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTA                             >  SRR19973074.2855597/1‑150 (MQ=60)
                                                                                                                         GACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATG                          >  SRR19973074.1408444/1‑150 (MQ=60)
                                                                                                                         GACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATG                          >  SRR19973074.2017628/1‑150 (MQ=60)
                                                                                                                         GACGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATG                          >  SRR19973074.2017639/1‑150 (MQ=60)
                                                                                                                           CGGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCC                        >  SRR19973074.4168150/1‑150 (MQ=60)
                                                                                                                            GGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCA                       <  SRR19973074.2878365/150‑1 (MQ=60)
                                                                                                                            GGGCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCA                       >  SRR19973074.4131485/1‑150 (MQ=60)
                                                                                                                              GCGCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAAC                     <  SRR19973074.499284/150‑1 (MQ=60)
                                                                                                                                GCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAA                   <  SRR19973074.2730869/150‑1 (MQ=60)
                                                                                                                                GCAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAA                   >  SRR19973074.3981987/1‑150 (MQ=60)
                                                                                                                                 CAGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAA                  >  SRR19973074.3877180/1‑150 (MQ=60)
                                                                                                                                  AGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAA                 >  SRR19973074.3546612/1‑150 (MQ=60)
                                                                                                                                  AGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAA                 >  SRR19973074.1104768/1‑150 (MQ=60)
                                                                                                                                  AGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAA                 >  SRR19973074.1104687/1‑150 (MQ=60)
                                                                                                                                  AGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAA                 >  SRR19973074.2810015/1‑150 (MQ=60)
                                                                                                                                  AGTCCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAA                 >  SRR19973074.2810028/1‑150 (MQ=60)
                                                                                                                                     CCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGG              <  SRR19973074.4140515/150‑1 (MQ=60)
                                                                                                                                     CCCGCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGG              <  SRR19973074.3820207/150‑1 (MQ=60)
                                                                                                                                        GCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGC           <  SRR19973074.4132550/150‑1 (MQ=60)
                                                                                                                                        GCCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGC           >  SRR19973074.2774548/1‑150 (MQ=60)
                                                                                                                                         CCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCG          <  SRR19973074.2025256/150‑1 (MQ=60)
                                                                                                                                         CCTGTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCG          <  SRR19973074.3711971/150‑1 (MQ=60)
                                                                                                                                            GTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCT       >  SRR19973074.3942796/1‑150 (MQ=60)
                                                                                                                                            GTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCT       >  SRR19973074.3475549/1‑150 (MQ=60)
                                                                                                                                            GTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCT       >  SRR19973074.759244/1‑150 (MQ=60)
                                                                                                                                            GTTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCT       <  SRR19973074.1151232/150‑1 (MQ=60)
                                                                                                                                             TTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTG      <  SRR19973074.2737617/150‑1 (MQ=60)
                                                                                                                                             TTCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTG      <  SRR19973074.2737592/150‑1 (MQ=60)
                                                                                                                                              TCCATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGG     >  SRR19973074.644167/1‑150 (MQ=60)
                                                                                                                                               CCATCTTTTTTTCACATTGCGCACCATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGG    <  SRR19973074.431072/150‑1 (MQ=60)
                                                                                                                                                CATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGC   >  SRR19973074.1065727/1‑150 (MQ=60)
                                                                                                                                                 ATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  SRR19973074.3391081/1‑150 (MQ=60)
                                                                                                                                                 ATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  SRR19973074.3511347/1‑150 (MQ=60)
                                                                                                                                                 ATCTTTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  SRR19973074.1197629/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
GATCATCAGACCAATAATTGAGCGATTGCCATCTTTGATTTGAGCAACCACGGATTCTGCCACCGCAGGCTGACGGCGATAATCTTTATTGGAATTACCGTGGCTGCAATCTACCATCAGAGACGGGCGCAGTCCCGCCTGTTCCATCTCTTTTTCACATTGCGCAACATCCGCAGGGCTATAGTTCGGCGCTTTACCACCGCGCAGGATCACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCG  >  CP009273/2733604‑2733898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑