Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,138,648 T→A 6.2% N325Y (AAC→TAC)  hybO ← hydrogenase 2, small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,138,6480TA 6.2% 1057.0 / 39.9 334N325Y (AAC→TAC) hybOhydrogenase 2, small subunit
Reads supporting (aligned to +/- strand):  ref base T (153/160);  new base A (9/12);  total (162/172)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

CGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGC  >  CP009273/3138499‑3138796
                                                                                                                                                     |                                                                                                                                                    
 ggTTATTCTCCCCGTGAGTCGGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTa                                                                                                                                                     >  2:4463480/1‑150 (MQ=255)
 ggTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTa                                                                                                                                                     <  2:4762228/150‑1 (MQ=255)
 ggTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTa                                                                                                                                                     <  2:3521937/150‑1 (MQ=255)
  gTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTaa                                                                                                                                                    <  1:2947221/150‑1 (MQ=255)
  gTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTaa                                                                                                                                                    >  1:3907113/1‑150 (MQ=255)
    tATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACa                                                                                                                                                  >  1:2168341/1‑150 (MQ=255)
      ttCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATc                                                                                                                                                >  1:4449880/1‑150 (MQ=255)
         tcCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCgg                                                                                                                                             <  2:1160979/150‑1 (MQ=255)
            ccGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGttt                                                                                                                                          <  2:4785973/150‑1 (MQ=255)
            ccGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGttt                                                                                                                                          <  1:1152782/150‑1 (MQ=255)
              gTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTGTAGCGTTAACATCCGGTTTCt                                                                                                                                        >  2:2393926/1‑150 (MQ=255)
               tGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCtg                                                                                                                                       <  1:2940135/150‑1 (MQ=255)
                gAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCtgt                                                                                                                                      >  2:237501/1‑150 (MQ=255)
                       cGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAAcgcg                                                                                                                               <  1:5126510/150‑1 (MQ=255)
                        gTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCgg                                                                                                                              >  2:4931883/1‑150 (MQ=255)
                             ctttctttTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGttt                                                                                                                         >  1:2006291/1‑150 (MQ=255)
                             ctttctttTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGttt                                                                                                                         >  1:5163644/1‑150 (MQ=255)
                               ttctttTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGa                                                                                                                       >  1:187719/1‑150 (MQ=255)
                                 gttttGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGAtt                                                                                                                     <  1:3562601/149‑1 (MQ=255)
                                 ctttTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGAtt                                                                                                                     <  2:3188244/150‑1 (MQ=255)
                                    ttGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTc                                                                                                                  >  1:3816935/1‑150 (MQ=255)
                                       cTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGAc                                                                                                               <  1:1000989/150‑1 (MQ=255)
                                          acgacCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGtt                                                                                                            <  1:2820883/150‑1 (MQ=255)
                                           cgacCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTg                                                                                                           <  1:689673/150‑1 (MQ=255)
                                           cgacCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTg                                                                                                           <  2:821589/150‑1 (MQ=255)
                                             acCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGc                                                                                                         <  2:5075593/150‑1 (MQ=255)
                                             acCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGc                                                                                                         <  2:1109917/150‑1 (MQ=255)
                                               ccAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCG‑CCGCATTAGCCTTAAAAACCCGGTTAATTGAACGCCGTGTTTGTTTTTCGACGTTTGCAAg                                                                                                      >  2:1265624/1‑150 (MQ=255)
                                                 aGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGc                                                                                                     >  2:2232714/1‑150 (MQ=255)
                                                 aGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTGTCGACGTTGGCACGc                                                                                                     >  1:3512983/1‑150 (MQ=255)
                                                  gTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCt                                                                                                    >  2:423835/1‑150 (MQ=255)
                                                   ttCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACCCCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTg                                                                                                   <  1:4797667/150‑1 (MQ=255)
                                                      aCGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatg                                                                                                <  2:2086331/150‑1 (MQ=255)
                                                      aCGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatg                                                                                                >  1:4701585/1‑150 (MQ=255)
                                                        gCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGACTGCAGAGACGTTGCCGCCCTATTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatgga                                                                                              <  2:2547398/150‑1 (MQ=255)
                                                         cACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatggat                                                                                             <  2:3756910/150‑1 (MQ=255)
                                                         cACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatggat                                                                                             <  2:2984685/150‑1 (MQ=255)
                                                          aCCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatggatg                                                                                            >  2:3995377/1‑150 (MQ=255)
                                                            cGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGcc                                                                                          >  1:3349354/1‑150 (MQ=255)
                                                             gCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCt                                                                                         >  2:69835/1‑150 (MQ=255)
                                                             gCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCt                                                                                         <  2:2516886/150‑1 (MQ=255)
                                                              ccATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCtt                                                                                        >  2:2815571/1‑150 (MQ=255)
                                                               cATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCttt                                                                                       <  1:55404/150‑1 (MQ=255)
                                                               cATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCttt                                                                                       <  1:1384302/150‑1 (MQ=255)
                                                                aTCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTa                                                                                      <  2:4497126/150‑1 (MQ=255)
                                                                aTCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTa                                                                                      <  2:1779892/150‑1 (MQ=255)
                                                                  cACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATg                                                                                    >  2:325902/1‑150 (MQ=255)
                                                                        gACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGcc                                                                              <  1:1187682/150‑1 (MQ=255)
                                                                        gACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGcc                                                                              <  2:938084/150‑1 (MQ=255)
                                                                         acacCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCNACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCg                                                                             <  1:2679694/150‑1 (MQ=255)
                                                                            ccGGCAACCAACCCAACCACACCGCCGAGCAAACCAATCGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATa                                                                          <  1:208025/150‑1 (MQ=255)
                                                                             cGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGTCAAGCTGATGGATGCCTTTATGGAAGCCGATAc                                                                         <  1:3042178/150‑1 (MQ=255)
                                                                              ggCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATAcc                                                                        >  1:3451198/1‑150 (MQ=255)
                                                                                 aaccaaccCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACcttc                                                                     <  1:533246/150‑1 (MQ=255)
                                                                                  accaaccCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACcttct                                                                    >  1:3844526/1‑150 (MQ=255)
                                                                                   ccaacccaacCACACCGCCGAGCAAACCACTAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACcttctt                                                                   <  2:3396963/150‑1 (MQ=255)
                                                                                    caacccaaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCttcttc                                                                  <  1:178628/150‑1 (MQ=255)
                                                                                      acccaaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGt                                                                >  1:1492377/1‑150 (MQ=255)
                                                                                       cccaaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGtt                                                               >  1:5039986/1‑150 (MQ=255)
                                                                                        ccaaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTa                                                              <  1:237501/150‑1 (MQ=255)
                                                                                          aaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACa                                                            <  1:3173087/150‑1 (MQ=255)
                                                                                            ccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGc                                                          >  2:3929507/1‑150 (MQ=255)
                                                                                            ccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGc                                                          <  1:2651309/150‑1 (MQ=255)
                                                                                                  cgccgAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGc                                                    >  1:1739518/1‑150 (MQ=255)
                                                                                                  cgccgAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTAGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGc                                                    >  1:2252106/1‑150 (MQ=255)
                                                                                                   gccgAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCa                                                   >  1:4068613/1‑150 (MQ=255)
                                                                                                    ccgAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCaa                                                  >  2:2153466/1‑150 (MQ=255)
                                                                                                     cgAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACCTCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAg                                                 <  2:2780597/150‑1 (MQ=255)
                                                                                                      gAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAgg                                                >  2:1706294/1‑150 (MQ=255)
                                                                                                       aGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAggg                                               <  1:2851754/150‑1 (MQ=255)
                                                                                                       aGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAggg                                               <  1:1747805/150‑1 (MQ=255)
                                                                                                        gCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGt                                              <  2:2908277/150‑1 (MQ=255)
                                                                                                          aaaCCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGa                                            <  2:4659291/150‑1 (MQ=255)
                                                                                                          aaaCCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGa                                            >  1:3387586/1‑150 (MQ=255)
                                                                                                           aaCCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGAc                                           <  2:1146285/150‑1 (MQ=255)
                                                                                                            aCCAATAGCGCCTGCAGAGACGTTGTCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGAcc                                          <  1:1246592/150‑1 (MQ=255)
                                                                                                              cAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCaa                                        <  2:76432/150‑1 (MQ=255)
                                                                                                               aaTAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAAt                                       <  2:4079500/150‑1 (MQ=255)
                                                                                                                 tAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCg                                     >  1:4164486/1‑150 (MQ=255)
                                                                                                                  aGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGc                                    >  2:1885069/1‑150 (MQ=255)
                                                                                                                   gcgcCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCTGTTTCTGTGAACGCGGAGTTTGTTTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGTGTGACCATTCGcc                                   <  2:1406193/150‑1 (MQ=255)
                                                                                                                      ccTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCAcc                                <  1:716597/150‑1 (MQ=255)
                                                                                                                                cGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATTGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCacacac                      >  2:908361/1‑150 (MQ=255)
                                                                                                                                cGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCacacac                      >  2:602333/1‑150 (MQ=255)
                                                                                                                                cGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCacacac                      <  2:271092/150‑1 (MQ=255)
                                                                                                                                cGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCacacac                      >  2:456254/1‑150 (MQ=255)
                                                                                                                                 gTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACAcc                     >  2:362407/1‑150 (MQ=255)
                                                                                                                                   tGCCGCCCTCTTTAGCGTTAACATCCTGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGATGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGTGTGACCAATCGCCACCGGCCACACACCGc                   >  1:1910569/1‑150 (MQ=255)
                                                                                                                                   tGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGc                   >  1:4645499/1‑150 (MQ=255)
                                                                                                                                      cgccCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCaa                >  1:3709421/1‑150 (MQ=255)
                                                                                                                                         ccTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACAt             <  1:745652/150‑1 (MQ=255)
                                                                                                                                         ccTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACAt             <  2:3592886/150‑1 (MQ=255)
                                                                                                                                         ccTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACAt             >  1:4553306/1‑150 (MQ=255)
                                                                                                                                           tctTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCg           <  1:922347/150‑1 (MQ=255)
                                                                                                                                           tctTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCg           >  1:4089049/1‑150 (MQ=255)
                                                                                                                                             tttAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCa         <  2:4428218/150‑1 (MQ=255)
                                                                                                                                             tttAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCa         >  1:4269852/1‑150 (MQ=255)
                                                                                                                                              ttAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAg        <  1:3337504/150‑1 (MQ=255)
                                                                                                                                                  cGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAAtt    <  1:2343835/150‑1 (MQ=255)
                                                                                                                                                   gTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGACCACACACCGCCAACATCGCAGAATTg   >  1:617925/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGC  >  CP009273/3138499‑3138796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A