Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,138,648 T→A 25.0% N325Y (AAC→TAC)  hybO ← hydrogenase 2, small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,138,6480TA25.0% 538.1 / 201.8 284N325Y (AAC→TAC) hybOhydrogenase 2, small subunit
Reads supporting (aligned to +/- strand):  ref base T (106/107);  new base A (37/34);  total (143/141)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 291 total aligned reads displayed.

GGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCA  >  CP009273/3138500‑3138797
                                                                                                                                                    |                                                                                                                                                     
ggTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTa                                                                                                                                                      >  1:1349685/1‑150 (MQ=255)
ggTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTa                                                                                                                                                      >  1:3962244/1‑150 (MQ=255)
ggTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTa                                                                                                                                                      >  2:4749125/1‑150 (MQ=255)
ggTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTa                                                                                                                                                      >  2:127511/1‑150 (MQ=255)
    aTTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACAt                                                                                                                                                  <  1:2159406/150‑1 (MQ=255)
    aTTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACAt                                                                                                                                                  <  1:3165078/150‑1 (MQ=255)
      tctcCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATcc                                                                                                                                                <  1:510517/150‑1 (MQ=255)
      tctcCCCGAGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATcc                                                                                                                                                <  2:1039675/150‑1 (MQ=255)
           ccGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGttt                                                                                                                                           <  2:1727319/150‑1 (MQ=255)
           ccGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGttt                                                                                                                                           <  2:3711342/150‑1 (MQ=255)
            cGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTc                                                                                                                                          >  2:3692074/1‑150 (MQ=255)
            cGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTc                                                                                                                                          <  2:4838505/150‑1 (MQ=255)
              tGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCtg                                                                                                                                        >  2:2343351/1‑150 (MQ=255)
                 gTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGa                                                                                                                                     >  1:5262167/1‑150 (MQ=255)
                   cAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAAc                                                                                                                                   >  1:3829748/1‑150 (MQ=255)
                   cAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGATGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGacc                                                                                                                                   >  2:3681883/1‑148 (MQ=255)
                    aGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGACcg                                                                                                                                  >  2:3504544/1‑150 (MQ=255)
                    aGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAAcg                                                                                                                                  >  1:1172099/1‑150 (MQ=255)
                     gCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAAcgc                                                                                                                                 <  2:5227745/150‑1 (MQ=255)
                          aTCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGt                                                                                                                            >  2:663885/1‑150 (MQ=255)
                                ctttTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGAtt                                                                                                                      <  1:4658266/150‑1 (MQ=255)
                                ctttTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGAtt                                                                                                                      >  1:4324161/1‑150 (MQ=255)
                                  tttGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGAtttt                                                                                                                    <  2:2125661/150‑1 (MQ=255)
                                     gCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGa                                                                                                                 >  1:1018085/1‑150 (MQ=255)
                                      cTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGAc                                                                                                                >  1:1873007/1‑150 (MQ=255)
                                         acgacCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGtt                                                                                                             >  2:4177902/1‑150 (MQ=255)
                                         acgacCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGtt                                                                                                             <  2:2660713/150‑1 (MQ=255)
                                          cgacCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTg                                                                                                            >  1:3267278/1‑150 (MQ=255)
                                             cccAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCa                                                                                                         >  1:3559370/1‑150 (MQ=255)
                                              ccAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCaa                                                                                                        >  2:327447/1‑150 (MQ=255)
                                               cAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAg                                                                                                       >  2:5142628/1‑150 (MQ=255)
                                                aGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCACGc                                                                                                      >  2:288622/1‑150 (MQ=255)
                                                aGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTGTCTGTGAACGCGGAGTTTGAGTTTCGACGTTGGCAAGc                                                                                                      >  2:3912064/1‑150 (MQ=255)
                                                 gTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCt                                                                                                     <  2:3360629/150‑1 (MQ=255)
                                                   tCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTga                                                                                                   >  2:155877/1‑150 (MQ=255)
                                                    cACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgat                                                                                                  <  2:3164150/150‑1 (MQ=255)
                                                    cACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgat                                                                                                  <  2:2703009/150‑1 (MQ=255)
                                                    cACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgat                                                                                                  >  1:2861069/1‑150 (MQ=255)
                                                     aCGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatg                                                                                                 <  2:461379/150‑1 (MQ=255)
                                                     aCGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatg                                                                                                 >  1:2436268/1‑150 (MQ=255)
                                                       gCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatgga                                                                                               >  1:1923201/1‑150 (MQ=255)
                                                       gCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGGTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatgga                                                                                               >  2:3118433/1‑150 (MQ=255)
                                                        cACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatggat                                                                                              <  2:1125060/150‑1 (MQ=255)
                                                        cACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTgatggat                                                                                              <  2:2215820/150‑1 (MQ=255)
                                                            gCCATCACGCTGACACCGGCAACCAACCCACCCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCt                                                                                          <  1:2614173/150‑1 (MQ=255)
                                                            gCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCt                                                                                          >  1:924206/1‑150 (MQ=255)
                                                            gCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCt                                                                                          <  2:4025646/150‑1 (MQ=255)
                                                            gCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCt                                                                                          <  2:304279/150‑1 (MQ=255)
                                                            gCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCt                                                                                          >  1:339060/1‑150 (MQ=255)
                                                             ccATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCtt                                                                                         <  2:539999/150‑1 (MQ=255)
                                                             ccATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCtt                                                                                         <  1:2343351/150‑1 (MQ=255)
                                                              cATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCttt                                                                                        >  2:1190196/1‑150 (MQ=255)
                                                              cATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCttg                                                                                        >  2:1586355/1‑149 (MQ=255)
                                                                tCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTAt                                                                                      <  1:4923228/150‑1 (MQ=255)
                                                                tCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTAt                                                                                      <  1:971993/150‑1 (MQ=255)
                                                                     cTGACACCGGCAACCAACCCACCCCCACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAg                                                                                 <  2:1333556/150‑1 (MQ=255)
                                                                           ccGGCAACCAACCCACCCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATa                                                                           <  1:2496462/150‑1 (MQ=255)
                                                                           ccGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATa                                                                           <  2:2416876/150‑1 (MQ=255)
                                                                            cGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATAc                                                                          <  2:2201092/150‑1 (MQ=255)
                                                                             ggCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATAcc                                                                         >  2:4368156/1‑150 (MQ=255)
                                                                              gCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACct                                                                        <  1:360366/150‑1 (MQ=255)
                                                                                   caacccaaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCttcttc                                                                   <  1:1602719/150‑1 (MQ=255)
                                                                                    aacccacccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCg                                                                  <  1:2032243/150‑1 (MQ=255)
                                                                                       ccaaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTa                                                               >  2:1962649/1‑150 (MQ=255)
                                                                                        caaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTAc                                                              <  1:104833/150‑1 (MQ=255)
                                                                                        caaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTAc                                                              <  2:2738941/150‑1 (MQ=255)
                                                                                         aaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACa                                                             <  1:2226509/150‑1 (MQ=255)
                                                                                         aaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACa                                                             <  2:2513044/150‑1 (MQ=255)
                                                                                         aaccACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGGTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACa                                                             <  1:5180764/150‑1 (MQ=255)
                                                                                            cacaCCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGcc                                                          >  2:4787786/1‑150 (MQ=255)
                                                                                                       gCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGt                                               >  1:4210258/1‑150 (MQ=255)
                                                                                                        cAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTg                                              >  1:3454350/1‑150 (MQ=255)
                                                                                                         aaaCCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGa                                             >  2:748919/1‑150 (MQ=255)
                                                                                                         aaaCCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGa                                             >  2:4702671/1‑150 (MQ=255)
                                                                                                          aaCCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGAc                                            >  2:4245198/1‑150 (MQ=255)
                                                                                                                tAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCg                                      <  2:946214/150‑1 (MQ=255)
                                                                                                                tAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCg                                      <  1:4943923/150‑1 (MQ=255)
                                                                                                                     ccTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCAcc                                 <  2:930802/150‑1 (MQ=255)
                                                                                                                          agagACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCca                            >  2:2640217/1‑150 (MQ=255)
                                                                                                                          agagACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCca                            >  1:3046748/1‑150 (MQ=255)
                                                                                                                                gTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACAcc                      <  2:2590552/150‑1 (MQ=255)
                                                                                                                                 ttGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCg                     >  2:913923/1‑150 (MQ=255)
                                                                                                                                 ttGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCg                     <  2:3416618/150‑1 (MQ=255)
                                                                                                                                   gccgccCTCTTTCGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACAGcc                   <  2:2994717/150‑1 (MQ=255)
                                                                                                                                    ccgccCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCa                  >  2:3451385/1‑150 (MQ=255)
                                                                                                                                    ccgccCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCa                  >  2:5021219/1‑150 (MQ=255)
                                                                                                                                      gccCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAAc                >  2:4691198/1‑150 (MQ=255)
                                                                                                                                       cccTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACa               >  1:574048/1‑150 (MQ=255)
                                                                                                                                        ccTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACAt              <  1:3528895/150‑1 (MQ=255)
                                                                                                                                         ctctTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATc             >  1:620509/1‑150 (MQ=255)
                                                                                                                                         ctctTTAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACAGCCAACATc             >  1:453181/1‑150 (MQ=255)
                                                                                                                                          tctTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCg            <  1:2968851/150‑1 (MQ=255)
                                                                                                                                            tttAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCa          >  2:139554/1‑150 (MQ=255)
                                                                                                                                             ttAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAg         >  1:696805/1‑150 (MQ=255)
                                                                                                                                              tAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGa        <  1:2703785/150‑1 (MQ=255)
                                                                                                                                              tAGCGTAAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACAGCCAACATCGCAGa        >  2:4533582/1‑150 (MQ=255)
                                                                                                                                                 cGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAAtt     >  2:122144/1‑150 (MQ=255)
                                                                                                                                                  gTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTg    >  2:1821560/1‑150 (MQ=255)
                                                                                                                                                   ttAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGc   <  1:4177902/150‑1 (MQ=255)
                                                                                                                                                    tAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCa  <  2:1908819/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCGAGCAAACCAATAGCGCCTGCAGAGACGTTGCCGCCCTCTTTAGCGTTAACATCCGGTTTCTGTGAACGCGGAGTTTGATTTTCGACGTTGGCAAGCTGATGGATGCCTTTATGGAAGCCGATACCTTCTTCGTTACAGCCATAGCAAGGGTGACCAATCGCCACCGGCCACACACCGCCAACATCGCAGAATTGCA  >  CP009273/3138500‑3138797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A